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Gene Dmel\wah

General Information
Symbol	Dmel\wah	Species	D. melanogaster
Name	waharan	Annotation symbol	CG4699
Feature type	protein_coding_gene	FlyBase ID	FBgn0262527
Gene Model Status	Current	Stock availability	17 publicly available
Also Known As	NSL1
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	89A4-89A5	Sequence location	3R:11,619,504..11,627,824 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene waharan is referred to in FlyBase by the symbol Dmel\wah (CG4699, FBgn0262527). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: germ-line stem cell maintenance; larval somatic muscle development; regulation of endocytosis; mitotic G2 DNA damage checkpoint; oogenesis; neuromuscular junction development; germarium-derived female germ-line cyst formation. 19 alleles are reported. The phenotypes of these alleles are annotated with: eye; neuromuscular junction; wing; wing vein. It has 12 annotated transcripts and 12 annotated polypeptides. Protein features are: KAT8 regulatory NSL complex subunit 1. Gene sequence location is 3R:11619504..11627824.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89A4-89A5   Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 89A4-89A5   (determined by in situ hybridisation)   (Spradling et al., 1999) 89A4-89A5     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	3- (Lone et al., 2006)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\wah for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.41 Gene model reviewed during 5.47 DGC clone appears problematic (LP09056): incomplete CDS.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) wah-RD FBtr0301726  NM_001170150   5102   1550 wah-RE FBtr0301727  NM_001170151   6051   1549 wah-RF FBtr0301728  NM_142235   5877   1548 wah-RH FBtr0301730  NM_001170152   5907   1550 wah-RI FBtr0301731  NM_169673   5701   1550 wah-RJ FBtr0301732  NM_001170153   6120   936 wah-RK FBtr0309859  NM_001260194   5172   982 wah-RL FBtr0309860  NM_001260195   5874   981 wah-RM FBtr0309861  NM_169672   5262   1570 wah-RN FBtr0309862  NM_001260196   5159   1569 wah-RO FBtr0309863  NM_001260197   5906   1551 wah-RP FBtr0334592  NM_001275684   5981   1549
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein wah-PD   FBpp0290940   167.9   1550   6.80     NP_001163621   ACZ94917 wah-PE   FBpp0290941   167.8   1549   6.80     NP_001163622   ACZ94918 wah-PF   FBpp0290942   167.7   1548   6.80     NP_650492   AAF55226 wah-PH   FBpp0290944   167.9   1550   6.80     NP_001163623   ACZ94919 wah-PI   FBpp0290945   167.9   1550   6.80     NP_732070   AAN13677 wah-PJ   FBpp0290946   99.1   936   7.88     NP_001163624   ACZ94920 wah-PK   FBpp0301593   104.0   982   7.88     NP_001247123   AFH06441 wah-PL   FBpp0301594   104.0   981   7.88     NP_001247124   AFH06442 wah-PM   FBpp0301595   169.8   1570   6.80     NP_732069   AAF55225 wah-PN   FBpp0301596   169.7   1569   6.80     NP_001247125   AFH06443 wah-PO   FBpp0301597   168.0   1551   6.80     NP_001247126   AFH06444 wah-PP   FBpp0306659   167.8   1549   6.80     NP_001262613   AGB95993
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • KAT8 regulatory NSL complex subunit 1 (IPR026180)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264908   AA440964   AA538779   AA736036   AA803644   AA949822   AE014297 AAF55225   AE014297 AAF55226   AE014297 AAN13677   AE014297 ACZ94917   AE014297 ACZ94918   AE014297 ACZ94919   AE014297 ACZ94920   AE014297 AFH06441   AE014297 AFH06442   AE014297 AFH06443   AE014297 AFH06444   AE014297 AGB95993   AI133839   AI259382   AI293450   AI293767   AI295430   AI296615   AI387693   AI388627   AI455775   AI514492   AI515981   AI541689   AI541740   AI542822   AI546156   AQ034070   AW944109   AY231719   BF500790   BG633376   BG638572   BG639017   BI234406   BI238990   BI353300   BI366397   BI632472   BI636627   BI638946   BP557387   BP557388   BT011104   BT044451   CK605451   CK605452   CK659423   CO268927   CO272393   CO276314   CO282546   CO285159   CO292871   CO294229   CO322452   CO323489   CO326077   CO329219   CO331881   CO340006   CO342255   CZ469588   EC057741   EC059613   EC078650   EC082932   EC086106   EC208717   EC223496   EC235633   EC247516   EC251054   EC259225   EV598115   EV599984   FM867600   FM867601   FM867602   FM867603   GH751071   GH771599   GH799314   GH799999   GH810389   GH830671   GH845348   GH846726   GH848865   GH851657   GH877324   GH879552   GH893647   GH933372   GH937504   GH938182   GH939313
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q0KI68 A4V2Z1 E1JIM0 E1JIM1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasmic part inferred from direct assay (Lone et al., 2010) nucleus inferred from direct assay (Lone et al., 2010, Yu et al., 2010)
Expression Deduced from Reporters
Reporter: P{PZ}wah06536 Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage   imaginal disc   (Meister and Braun, 1995.10) embryonic/larval brain   (Meister and Braun, 1995.10) embryonic/larval fat body   (Meister and Braun, 1995.10) adult stage   adult brain | restricted   (Vosshall, 1998.8.10) adult fat body   (Vosshall, 1998.8.10) adult central complex   (Vosshall, 1998.8.10)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    51 central nervous system, pupae P8    31 head, virgin 1-day female    18 head, virgin 4-day female    24 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    17 head, mated 1-day male    28 head, mated 4-day male    29 head, mated 20-day male    30 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    10 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    7 fat body, white prepupae    12 fat body, pupae P8    16 carcass, larvae L3 wandering    25 carcass, 1-day adult    13 carcass, 4-day adult    17 carcass, 20-day adult    15 ovary, virgin 4-day female    75 ovary, mated 4-day female    83 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (29) central nervous system, larvae L3  (51) central nervous system, pupae P8  (31) head, virgin 1-day female    18 head, virgin 4-day female    24 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    17 head, mated 1-day male  (28) head, mated 4-day male  (29) head, mated 20-day male  (30) salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    10 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    7 fat body, white prepupae    12 fat body, pupae P8    16 carcass, larvae L3 wandering    25 carcass, 1-day adult    13 carcass, 4-day adult    17 carcass, 20-day adult    15 ovary, virgin 4-day female  (75) ovary, mated 4-day female  (83) testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    51 central nervous system, pupae P8    31 head, virgin 1-day female    18 head, virgin 4-day female    24 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    17 head, mated 1-day male    28 head, mated 4-day male    29 head, mated 20-day male    30 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    10 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    7 fat body, white prepupae    12 fat body, pupae P8    16 carcass, larvae L3 wandering    25 carcass, 1-day adult    13 carcass, 4-day adult    17 carcass, 20-day adult    15 ovary, virgin 4-day female    75 ovary, mated 4-day female    83 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    51 central nervous system, pupae P8    31 head, virgin 1-day female    18 head, virgin 4-day female    24 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    17 head, mated 1-day male    28 head, mated 4-day male    29 head, mated 20-day male    30 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    10 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    7 fat body, white prepupae    12 fat body, pupae P8    16 carcass, larvae L3 wandering    25 carcass, 1-day adult    13 carcass, 4-day adult    17 carcass, 20-day adult    15 ovary, virgin 4-day female    75 ovary, mated 4-day female    83 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    51 central nervous system, pupae P8    31 head, virgin 1-day female    18 head, virgin 4-day female    24 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    17 head, mated 1-day male    28 head, mated 4-day male    29 head, mated 20-day male    30 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    10 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    7 fat body, white prepupae    12 fat body, pupae P8    16 carcass, larvae L3 wandering    25 carcass, 1-day adult    13 carcass, 4-day adult    17 carcass, 20-day adult    15 ovary, virgin 4-day female    75 ovary, mated 4-day female    83 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  29 central nervous system, larvae L3  (51) central nervous system, pupae P8  31 head, virgin 1-day female    18 head, virgin 4-day female    24 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    17 head, mated 1-day male  28 head, mated 4-day male  29 head, mated 20-day male  30 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    10 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    7 fat body, white prepupae    12 fat body, pupae P8    16 carcass, larvae L3 wandering    25 carcass, 1-day adult    13 carcass, 4-day adult    17 carcass, 20-day adult    15 ovary, virgin 4-day female  (75) ovary, mated 4-day female  (83) testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    51 central nervous system, pupae P8    31 head, virgin 1-day female    18 head, virgin 4-day female    24 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    17 head, mated 1-day male    28 head, mated 4-day male    29 head, mated 20-day male    30 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    10 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    7 fat body, white prepupae    12 fat body, pupae P8    16 carcass, larvae L3 wandering    25 carcass, 1-day adult    13 carcass, 4-day adult    17 carcass, 20-day adult    15 ovary, virgin 4-day female    75 ovary, mated 4-day female    83 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    51 central nervous system, pupae P8    31 head, virgin 1-day female    18 head, virgin 4-day female    24 head, virgin 20-day female    17 head, mated 1-day female    22 head, mated 4-day female    18 head, mated 20-day female    17 head, mated 1-day male    28 head, mated 4-day male    29 head, mated 20-day male    30 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    10 digestive system, 4-day adult    14 digestive system, 20-day adult    14 fat body, larvae L3 wandering    7 fat body, white prepupae    12 fat body, pupae P8    16 carcass, larvae L3 wandering    25 carcass, 1-day adult    13 carcass, 4-day adult    17 carcass, 20-day adult    15 ovary, virgin 4-day female    75 ovary, mated 4-day female    83 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    31 embryo 04-06hr    37 embryo 06-08hr    44 embryo 08-10hr    32 embryo 10-12hr    24 embryo 12-14hr    22 embryo 14-16hr    23 embryo 16-18hr    21 embryo 18-20hr    19 embryo 20-22hr    18 embryo 22-24hr    18 larva L1    20 larva L2    12 larva L3 12hr old    9 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    16 white prepupae new    17 white prepupae 12hr    19 white prepupae 24hr    23 pupae 2d postWPP    16 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    36 adult female 30day    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (58) embryo 02-04hr  (31) embryo 04-06hr  (37) embryo 06-08hr  (44) embryo 08-10hr  (32) embryo 10-12hr    24 embryo 12-14hr    22 embryo 14-16hr    23 embryo 16-18hr    21 embryo 18-20hr    19 embryo 20-22hr    18 embryo 22-24hr    18 larva L1    20 larva L2    12 larva L3 12hr old    9 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    16 white prepupae new    17 white prepupae 12hr    19 white prepupae 24hr    23 pupae 2d postWPP    16 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day  (36) adult female 30day  (37) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    31 embryo 04-06hr    37 embryo 06-08hr    44 embryo 08-10hr    32 embryo 10-12hr    24 embryo 12-14hr    22 embryo 14-16hr    23 embryo 16-18hr    21 embryo 18-20hr    19 embryo 20-22hr    18 embryo 22-24hr    18 larva L1    20 larva L2    12 larva L3 12hr old    9 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    16 white prepupae new    17 white prepupae 12hr    19 white prepupae 24hr    23 pupae 2d postWPP    16 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    36 adult female 30day    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    31 embryo 04-06hr    37 embryo 06-08hr    44 embryo 08-10hr    32 embryo 10-12hr    24 embryo 12-14hr    22 embryo 14-16hr    23 embryo 16-18hr    21 embryo 18-20hr    19 embryo 20-22hr    18 embryo 22-24hr    18 larva L1    20 larva L2    12 larva L3 12hr old    9 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    16 white prepupae new    17 white prepupae 12hr    19 white prepupae 24hr    23 pupae 2d postWPP    16 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    36 adult female 30day    37 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    31 embryo 04-06hr    37 embryo 06-08hr    44 embryo 08-10hr    32 embryo 10-12hr    24 embryo 12-14hr    22 embryo 14-16hr    23 embryo 16-18hr    21 embryo 18-20hr    19 embryo 20-22hr    18 embryo 22-24hr    18 larva L1    20 larva L2    12 larva L3 12hr old    9 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    16 white prepupae new    17 white prepupae 12hr    19 white prepupae 24hr    23 pupae 2d postWPP    16 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    36 adult female 30day    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (58) embryo 02-04hr  31 embryo 04-06hr  (37) embryo 06-08hr  (44) embryo 08-10hr  32 embryo 10-12hr    24 embryo 12-14hr    22 embryo 14-16hr    23 embryo 16-18hr    21 embryo 18-20hr    19 embryo 20-22hr    18 embryo 22-24hr    18 larva L1    20 larva L2    12 larva L3 12hr old    9 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    16 white prepupae new    17 white prepupae 12hr    19 white prepupae 24hr    23 pupae 2d postWPP    16 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day  (36) adult female 30day  (37) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    31 embryo 04-06hr    37 embryo 06-08hr    44 embryo 08-10hr    32 embryo 10-12hr    24 embryo 12-14hr    22 embryo 14-16hr    23 embryo 16-18hr    21 embryo 18-20hr    19 embryo 20-22hr    18 embryo 22-24hr    18 larva L1    20 larva L2    12 larva L3 12hr old    9 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    16 white prepupae new    17 white prepupae 12hr    19 white prepupae 24hr    23 pupae 2d postWPP    16 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    36 adult female 30day    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    58 embryo 02-04hr    31 embryo 04-06hr    37 embryo 06-08hr    44 embryo 08-10hr    32 embryo 10-12hr    24 embryo 12-14hr    22 embryo 14-16hr    23 embryo 16-18hr    21 embryo 18-20hr    19 embryo 20-22hr    18 embryo 22-24hr    18 larva L1    20 larva L2    12 larva L3 12hr old    9 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    16 white prepupae new    17 white prepupae 12hr    19 white prepupae 24hr    23 pupae 2d postWPP    16 pupae 3d postWPP    11 pupae 4d postWPP    10 adult male 01day    13 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    36 adult female 30day    37 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    48 Schneider line 2 Sg4    29 embryonic 1182-4H    39 embryonic GM2    29 embryonic Kc167    39 embryonic S1    47 embryonic S3    40 leg disc CME L1    39 wing disc CME-W2    22 wing disc ML-DmD8    29 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    24 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    41 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    28 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    22 ovary fGS/OSS    70 ovary OSC    46 ovary OSS    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (48) Schneider line 2 Sg4  (29) embryonic 1182-4H  (39) embryonic GM2  (29) embryonic Kc167  (39) embryonic S1  (47) embryonic S3  (40) leg disc CME L1  (39) wing disc CME-W2    22 wing disc ML-DmD8  (29) wing disc ML-DmD9  (34) wing disc ML-DmD16-c3  (39) wing disc ML-DmD21    24 wing disc ML-DmD32  (54) haltere disc ML-DmD17-c3  (41) eye-antennal disc ML-DmD11  (30) antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1  (28) CNS ML-DmBG1-c1  (37) CNS ML-DmBG2-c2  (31) tumorous blood cells mbn2    22 ovary fGS/OSS  (70) ovary OSC  (46) ovary OSS  (27) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    48 Schneider line 2 Sg4    29 embryonic 1182-4H    39 embryonic GM2    29 embryonic Kc167    39 embryonic S1    47 embryonic S3    40 leg disc CME L1    39 wing disc CME-W2    22 wing disc ML-DmD8    29 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    24 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    41 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    28 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    22 ovary fGS/OSS    70 ovary OSC    46 ovary OSS    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    48 Schneider line 2 Sg4    29 embryonic 1182-4H    39 embryonic GM2    29 embryonic Kc167    39 embryonic S1    47 embryonic S3    40 leg disc CME L1    39 wing disc CME-W2    22 wing disc ML-DmD8    29 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    24 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    41 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    28 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    22 ovary fGS/OSS    70 ovary OSC    46 ovary OSS    27 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    48 Schneider line 2 Sg4    29 embryonic 1182-4H    39 embryonic GM2    29 embryonic Kc167    39 embryonic S1    47 embryonic S3    40 leg disc CME L1    39 wing disc CME-W2    22 wing disc ML-DmD8    29 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    24 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    41 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    28 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    22 ovary fGS/OSS    70 ovary OSC    46 ovary OSS    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (48) Schneider line 2 Sg4  29 embryonic 1182-4H  (39) embryonic GM2  29 embryonic Kc167  (39) embryonic S1  (47) embryonic S3  (40) leg disc CME L1  (39) wing disc CME-W2    22 wing disc ML-DmD8  29 wing disc ML-DmD9  (34) wing disc ML-DmD16-c3  (39) wing disc ML-DmD21    24 wing disc ML-DmD32  (54) haltere disc ML-DmD17-c3  (41) eye-antennal disc ML-DmD11  30 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1  28 CNS ML-DmBG1-c1  (37) CNS ML-DmBG2-c2  31 tumorous blood cells mbn2    22 ovary fGS/OSS  (70) ovary OSC  (46) ovary OSS  27 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    48 Schneider line 2 Sg4    29 embryonic 1182-4H    39 embryonic GM2    29 embryonic Kc167    39 embryonic S1    47 embryonic S3    40 leg disc CME L1    39 wing disc CME-W2    22 wing disc ML-DmD8    29 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    24 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    41 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    28 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    22 ovary fGS/OSS    70 ovary OSC    46 ovary OSS    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    48 Schneider line 2 Sg4    29 embryonic 1182-4H    39 embryonic GM2    29 embryonic Kc167    39 embryonic S1    47 embryonic S3    40 leg disc CME L1    39 wing disc CME-W2    22 wing disc ML-DmD8    29 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    39 wing disc ML-DmD21    24 wing disc ML-DmD32    54 haltere disc ML-DmD17-c3    41 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    28 CNS ML-DmBG1-c1    37 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    22 ovary fGS/OSS    70 ovary OSC    46 ovary OSS    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    22 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    61 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (35) cold shock, 4-day adult    22 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult  (30) Cadmium 100 mM 48 hrs, 4-day adult  (67) Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult  (50) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult  26 Ethanol 2.5% 3 hrs, larvae L3  (56) Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (61) Caffeine 25 mg/ml 48 hrs, 4-day adult  (32) Paraquat 5 mM 48 hrs, 4-day adult  (49) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    22 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    61 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    22 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    61 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    22 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    61 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (35) cold shock, 4-day adult    22 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult  30 Cadmium 100 mM 48 hrs, 4-day adult  (67) Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult  (50) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult  26 Ethanol 2.5% 3 hrs, larvae L3  (56) Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (61) Caffeine 25 mg/ml 48 hrs, 4-day adult  32 Paraquat 5 mM 48 hrs, 4-day adult  (49) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    22 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    61 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    35 cold shock, 4-day adult    22 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    61 Caffeine 25 mg/ml 48 hrs, 4-day adult    32 Paraquat 5 mM 48 hrs, 4-day adult    49 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_01 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal wah06536 wahS009413 lethal (with Df(3R)Exel8162) wah1 wah06536 wahc04892 lethal (with wah1) wah06536 wahc04892 lethal (with wah06536) wah1 wahS009413 lethal (with wahc04892) wah1 lethal (with wahS009413) wah06536 lethal | embryonic stage wahS009413 lethal | first instar larval stage | recessive wah1 lethal | germline clone | oogenesis stage wah1 lethal | recessive wah06536 wahj2E5 lethal | recessive | second instar larval stage wahc04892 viable wah02 Other Phenotypes Allele visible, with Scer\GAL4Bx-MS1096 wahdsRNA.Scer\UAS.WIZ visible, with Scer\GAL4ey.PU wahdsRNA.Scer\UAS.WIZ Phenotype manifest in Allele embryonic/larval somatic muscle | larval stage, with Scer\GAL4C57 wahNIG.4699R eye, with Scer\GAL4ey.PU wahdsRNA.Scer\UAS.WIZ eye photoreceptor cell | somatic clone wahj2E5 neuromuscular junction wahS009413 wing, with Scer\GAL4Bx-MS1096 wahdsRNA.Scer\UAS.WIZ wing vein, with Scer\GAL4Bx-MS1096 wahdsRNA.Scer\UAS.WIZ
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of wah Class Mutagen Stocks Known lesion wahS009413 3 Yes wahj2E5 P-element activity 2 -- wah02 1 -- wah5-HA-1754 1 -- wah5-SZ-3416 1 -- wahc04892 piggyBac transposase 1 -- wahEP3447 1 -- wahNP7517 1 -- wah06536 P-element activity 0 Yes wah1 0 Yes
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of wah Class Mutagen Stocks Known lesion wahScer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 3 Yes wahNIG.4699R in vitro construct - regulatory fusion in vitro construct - RNAi 2 Yes wahScer\UAS.cLa in vitro construct - regulatory fusion 1 Yes wah+t9.9 in vitro construct - genomic fragment 0 Yes wahC.T:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes wahdsRNA.Scer\UAS.WIZ in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes wahGD11397 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes wahN.T:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes wahtPa in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6175 (Ryder, 2004.4.26) Df(3R)Exel8162 (Ryder, 2004.4.26, Yu et al., 2010) Df(3R)sbd105 (Spradling et al., 1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{UAS-wah.HA} NA P{UAS-wah.L} NA P{UAS-wah.WIZ} NA characterization construct P{NIG.4699R} NA P{wah9.9} NA P{wah-GFP.C} NA P{wah-GFP.N} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}wahEP3447 NA insertion of enhancer trap P{lacW}wahj2E5 No P{lacW}wahS009413 No P{PZ}wah06536 No miscellaneous insertions P{RS5}wah5-HA-1754 NA P{RS5}wah5-SZ-3416 NA P{UASp-YFP.Rab27.T25N}wah02 NA PBac{PB}wahc04892 NA insertion of enhancer trap binary system P{GawB}wahNP7517 No
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
germarium-derived female germ-line cyst formation inferred from genetic interaction with nos (Yu et al., 2010) germ-line stem cell maintenance inferred from genetic interaction with nos (Yu et al., 2010) larval somatic muscle development inferred from mutant phenotype (Lone et al., 2010) mitotic G2 DNA damage checkpoint inferred from genetic interaction with nbs (Kondo and Perrimon, 2011) neuromuscular junction development inferred from mutant phenotype (Lone et al., 2010, Lone et al., 2006) oogenesis inferred from genetic interaction with nos (Yu et al., 2010) regulation of endocytosis inferred from mutant phenotype (Lone et al., 2010)
Cellular Component
CV term Evidence References
cytoplasmic part inferred from direct assay (Lone et al., 2010) nucleus inferred from direct assay (Lone et al., 2010, Yu et al., 2010)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
histone acetyltransferase complex inferred from electronic annotation with InterPro:IPR026180 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
wah - wds anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions wah allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262527
Orthologs
OrthoDB Orthologs (13) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6JQ4GX Dipteran inclusive ortholog search EOG6WH8TV Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search EOG60CW9W
Orthologs in Drosophila Species (EOG6JQ4GX)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\wah     Drosophila simulans   Dsim\GD19048 Y   Drosophila sechellia   Dsec\GM24260 Y   Drosophila sechellia   Dsec\GM18136     Drosophila erecta   Dere\GG16953 Y   Drosophila yakuba   Dyak\GE24339 Y   Drosophila ananassae   Dana\GF18542 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18365 Y   Drosophila persimilis   Dper\GL22236 Y   Drosophila willistoni   Dwil\GK14063 Y   Drosophila virilis   Dvir\GJ23484 Y   Drosophila mojavensis   Dmoj\GI10196 Y
Orthologs in non-Drosophila Dipterans (EOG6WH8TV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ010301
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19048 Drosophila sechellia Dsec\GM24260 Drosophila erecta Dere\GG16953 Drosophila yakuba Dyak\GE24339 Drosophila ananassae Dana\GF18542 Drosophila pseudoobscura pseudoobscura Dpse\GA18365 Drosophila persimilis Dper\GL22236 Drosophila willistoni Dwil\GK14063 Drosophila virilis Dvir\GJ23484 Drosophila mojavensis Dmoj\GI10196 Drosophila grimshawi Dgri\GH18240
Stocks & Reagents
Stocks Listed in FlyBase ( 17 )
Bloomington	32561 w*; P{NIG.4699R}3 32656 w*; P{UAS-wah.L}1 32558 w*; P{UAS-wah.HA}4 33827 w*; P{UAS-wah.HA}7 32556 w*; P{UAS-wah.HA}7; P{GawB}C57 32559 w*; P{UAS-shrb-GFP}2; P{NIG.4699R}3 23267 y1 w*; P{UASp-YFP.Rab27.T25N}wah02 12304 y1 w1118; P{lacW}wahj2E5/TM3, Sb1
Harvard	- PBac{PB}wahc04892
Kyoto	125381 w1118; P{RS5}wah5-HA-1754 126046 w1118; P{RS5}wah5-SZ-3416 109015 w*; P{EP}wahEP3447 / TM6B, Tb1
	More stocks available...
Genomic Clones ( 2 )
	BACR06B08 BACR48G17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 82 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT07776 AT22722 GH19810 LP09056
Other clones	LD39557 LD46639
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT15825 GH10568 GH18285 GH19584 GH25781 GM10830 GM13765 GM23056 LD09594 LD12782 LD18382 LD35458 LD41018 LD46253 LP02779 LP06601 LP06980 LP10577 LP17175 RE30104 RE35368 RE51925 SD06703 SD06860 SD09290 SD18369 SD21353 SD26262
Other clones	62267 64016 72427 79032 178831 214257.46 274293 CK01.44C.A8 CK01.121D.D11 CK01.128A.C5 CK01.146C.B10 Dmel_neb_002297_0865_1597 Dmel_neb_011503_0950_1119 EK093114 EK107007 EK120339 EK160707 EK168401 EK188153 EK190610 EK201125 EK206357 EK292305 EK301914 EP03208 EP09852 EP14307 FGM309A08 LD29853 LD41709 RP003011479 RP003062792 RP003094035 RP003107909 RP003354314 RP003371577 RP003395782 RP003498407 RP003561628 RP003580172 RP003618181 RP003618701 RP003620833 RP003624200 RP003628119 RP003635288 RP003661419 UT01946
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0262527 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41911
Antibody Information
	polyclonal (Yu et al., 2010, Feller et al., 2012)
Other Information
Discoverer
Etymology
	'waharan' is a Kashmiri term for 'to expand'. (Lone et al., 2010)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: wah CG4699 (Lone et al., 2010) Source for merge of: wah l(3)06536 (Lone et al., 2010)
Additional comments
Other Comments
	wah mutants display neuromuscular junction overgrowth. The same phenotype occurs following dsRNA-mediated genetic interference of wah. (Lone et al., 2006) Identification: In a screen of lethal P-element insertions on the third chromosome that affect neuromuscular junctions. (Lone et al., 2006)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142235 NM_169672 NM_169673 NM_001170150 NM_001170151 NM_001170152 NM_001170153 NM_001260194 NM_001260195 NM_001260196 NM_001260197 NM_001275684 RefSeq (Proteins) • NP_650492 NP_732069 NP_732070 NP_001163621 NP_001163622 NP_001163623 NP_001163624 NP_001247123 NP_001247124 NP_001247125 NP_001247126 NP_001262613 DDBJ / EMBL / GenBank • AA538779 AAF55225 AAF55226 AAN13677 ACZ94917 ACZ94918 ACZ94919 ACZ94920 AFH06441 AFH06442 AFH06443 AFH06444 AGB95993 AI295430 AQ034070 AW944109 CZ469588 Entrez Gene - A searchable database of RefSeq genes. • 41911 UniProtKB/TrEMBL • A4V2Z1 E1JIM0 E1JIM1 Q0KI68
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • KAT8 regulatory NSL complex subunit 1 (IPR026180)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262527 DRSC - Results from RNAi screens. • FBgn0262527 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0262527 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41911 modMine - Data generated by the modENCODE project. • FBgn0262527
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG4699 (Larschan et al., 2012, Lone et al., 2010, Raja et al., 2010, Yu et al., 2008, Zhang et al., 2007, Mendjan et al., 2006, DasGupta et al., 2005, Lone et al., 2010, Wasbrough et al., 2010, Yu et al., 2010, Marin, 2003) E(nos) (Yu et al., 2010) l(3)06536 (Gambis et al., 2011) l(3)S009413 (Lone et al., 2010) NSL1 (Larschan et al., 2012, Raja et al., 2010, Prestel et al., 2010, Mendjan et al., 2006, Kondo and Perrimon, 2011, Dekanty et al., 2010, Koolen et al., 2012, Lam et al., 2012) wah (Lone et al., 2010, Lone, 2010.9.10, Lone et al., 2010, Friedman et al., 2011, Koolen et al., 2012)
Name Synonym	lethal (3) 06536   non-specific lethal 1 (Raja et al., 2010, Prestel et al., 2010) waharan (Lone, 2010.9.10, Lone et al., 2006, Lone et al., 2010, )
Secondary FlyBase IDs
	FBgn0010895 FBgn0038364 FBgn0085126 FBgn0262474
References ( 43 )
Generate a list of	All references relating to this gene ( 43 )
List References by type	Research paper  ( 20 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 2 ) FlyBase analysis  ( 2 )
Recent research papers ( 7 )
	Feller et al., 2012, Nucleic Acids Res. 40(4): 1509--1522 The MOF-containing NSL complex associates globally with housekeeping genes, but activates only a defined subset. [FBrf0217555] Koolen et al., 2012, Nat. Genet. 44(6): 639--641 Mutations in the chromatin modifier gene KANSL1 cause the 17q21.31 microdeletion syndrome. [FBrf0218489] Lam et al., 2012, PLoS Genet. 8(6): e1002736 The NSL Complex Regulates Housekeeping Genes in Drosophila. [FBrf0218697] Larschan et al., 2012, PLoS Genet. 8(7): e1002830 Identification of Chromatin-Associated Regulators of MSL Complex Targeting in Drosophila Dosage Compensation. [FBrf0219020] Friedman et al., 2011, Sci. Signal. 4(196): rs10 Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. [FBrf0216513] Gambis et al., 2011, Dev. Biol. 351(1): 128--134 Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila. [FBrf0213000] Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] Show all research papers ...