FlyBase Gene Report: Dmel\Shab

General Information

Symbol

Dmel\Shab

Species

D. melanogaster

Name

Shaker cognate b

Annotation Symbol

CG43128

Feature Type

protein_coding_gene

FlyBase ID

FBgn0262593

Gene Model Status

Stock Availability

16 publicly available

Gene Snapshot

Shaker cognate b (Shab) encodes a member of the Sh family and the structural alpha subunit of a delayed rectifier K[+] channel (Kv2). The product of Shab channel functions to regulate excitability in neurons and muscles, and transmitter release. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

dShab, Kv2

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:2,895,017..2,959,579 [+]

Recombination map

3-5

RefSeq locus

NT_037436 REGION:2895017..2959579

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables delayed rectifier potassium channel activity

inferred from direct assay

(Vahasoyrinki et al., 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables delayed rectifier potassium channel activity

inferred from biological aspect of ancestor with PANTHER:PTN001693752

(assigned by GO_Central )

(Gaudet et al., 2011)

enables voltage-gated potassium channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000164970

(assigned by GO_Central )

(Gaudet et al., 2011)

traceable author statement

(Littleton and Ganetzky, 2000)

Biological Process (9 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in action potential

inferred from mutant phenotype

(Ueda and Wu, 2006)

involved_in chemical synaptic transmission

inferred from mutant phenotype

(Yao and Wu, 2001)

involved_in larval locomotory behavior

inferred from mutant phenotype

(Ueda and Wu, 2006)

involved_in positive regulation of circadian sleep/wake cycle, sleep

inferred from mutant phenotype

(Pimentel et al., 2016)

involved_in regulation of heart contraction

inferred from mutant phenotype

(Frolov et al., 2008)

involved_in regulation of synaptic activity

inferred from mutant phenotype

(Ueda and Wu, 2006)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in potassium ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000164970

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in potassium ion transport

traceable author statement

(Littleton and Ganetzky, 2000)

involved_in protein homooligomerization

inferred from electronic annotation with InterPro:IPR003131

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in integral component of membrane

inferred from biological aspect of ancestor with PANTHER:PTN000164970

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in plasma membrane

inferred from sequence or structural similarity

(Butler et al., 1990)

inferred from sequence or structural similarity with FLYBASE:Sh; FB:FBgn0003380

(Butler et al., 1989)

part_of voltage-gated potassium channel complex

inferred from biological aspect of ancestor with PANTHER:PTN001355853

(assigned by GO_Central )

(Gaudet et al., 2011)

traceable author statement

(Littleton and Ganetzky, 2000)

Gene Group (FlyBase)

VOLTAGE-GATED POTASSIUM CHANNEL - ALPHA SUBUNITS

Protein Family (UniProt)

Belongs to the potassium channel family. B (Shab) (TC 1.A.1.2) subfamily. Shab sub-subfamily. (P17970)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

VOLTAGE-GATED POTASSIUM CHANNEL - ALPHA SUBUNITS -: Voltage-gated potassium α subunits form homo- or heterotetrameric transmembrane channels specific for potassium which are activated by changes in membrane potential. Kv channel α subunits possess six or seven transmembrane domains. (Adapted from FBrf0224790).

Protein Function (UniProtKB)

Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

(UniProt, P17970)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Shab: Shaker cognate b (J.C. Hall)

Expression in Xenopus oocytes reveals a delayed-rectifier type of potassium current expressed by Shab; the rate of current activation in Shab is somewhat faster than in Shaw.

Summary (Interactive Fly)

slow delayed rectifier K channel - upregulated by light stimulation - functions in light adaptation - regulates excitability in neurons and muscles, and transmitter release

Interactive Fly

Gene Model and Products

Number of Transcripts

10

Number of Unique Polypeptides

9

Please see the JBrowse view of Dmel\Shab for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 6.02

Contains low complexity sequences resulting in poly-glycine runs in the translation products.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0305201

4598

985

FBtr0305202

4586

1015

FBtr0305203

4756

1011

FBtr0305205

7439

1537

FBtr0305206

7461

1602

FBtr0305208

4439

972

FBtr0305207

5231

799

FBtr0305209

4553

1010

FBtr0333898

7596

1607

FBtr0333899

7491

1602

Additional Transcript Data and Comments

Reported size (kB)

6.8, 4.3 (northern blot)

(Butler et al., 1989)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0293731

106.6

985

6.28

FBpp0293732

109.8

1015

6.57

FBpp0293733

110.0

1011

6.41

FBpp0293735

164.6

1537

7.04

FBpp0293736

171.7

1602

6.57

FBpp0293738

105.2

972

6.95

FBpp0293737

87.3

799

6.79

FBpp0293739

109.6

1010

6.69

FBpp0306030

171.8

1607

6.49

FBpp0306031

171.7

1602

6.57

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1602 aa isoforms: Shab-PI, Shab-PN

Additional Polypeptide Data and Comments

Reported size (kDa)

955 (aa)

(Hegde et al., 1999)

924 (aa)

(Butler et al., 1990, Butler et al., 1989)

100 (kD predicted)

(Wei et al., 1990)

Comments

The sequence of Shab in Canton-S contains an inserted base relative to Oregon-R leading to a frameshift and a C-terminal extension of the protein.

(Hegde et al., 1999)

Co-injection experiments in Xenopus oocytes were used to test for formation of functional heterodimers between Sh subfamily members. In fact, no functional heterodimers (as shown by novel current kinetics) were detected when all pairwise combinations of the four different channel subfamilies (Sh, Shab, Shal, and Shaw) were tested. The independence of each channel system was retained even when all four were co-expressed.

(Covarrubias et al., 1991)

Shab protein was expressed in Xenopus oocytes and characterized with respect to kinetics (rate of macroscopic current activation and inactivation) and voltage sensitivity of steady-state inactivation. Those properties were compared among the Sh subfamily members and found to vary widely. Shab encodes the transient (A current)subtype of potassium channel and appears to function as a homomultimer.

(Wei et al., 1990)

External Data

Subunit Structure (UniProtKB)

Heterotetramer of potassium channel proteins.

(UniProt, P17970)

Domain

The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.

(UniProt, P17970)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Shab using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

(Butler et al., 1989)

embryonic stage 12 -- 17

(Butler et al., 1989)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Shab in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Shab

View exonic expression by tissue for Dmel\Shab

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0262593

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1066

Images

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Shab

Transgenic constructs containing regulatory region of Shab

Deletions and Duplications ( 10 )

Disrupted in

(Ryder, 2004.4.26)

(Chopra et al., 2000)

(Chopra et al., 2000)

Not disrupted in

(Chopra et al., 2000)

(Chopra et al., 2000)

(Chopra et al., 2000)

(Chopra et al., 2000)

(Chopra et al., 2000)

(Chopra et al., 2000)

Partially disrupted in

Df(3L)spz5³⁷

(Ulian-Benitez et al., 2017)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

Shab^JF01823

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Shab^GD5116

viable, with Scer\GAL4^Mef2.PR

Shab^GD5116

viable, with Scer\GAL4^pnr-MD237

Other Phenotypes

Allele

heat stress response defective | adult stage, with Scer\GAL4^elav-C155

Shab^JF01823

locomotor behavior defective | larval stage

Shab^unspecified

neurophysiology defective

neurophysiology defective, with Df(3L)HR370

neurophysiology defective (with Shab¹)

Shab²

neurophysiology defective (with Shab²)

Shab¹

neurophysiology defective | chemical conditional, with Scer\GAL4^eve.RRa

Shab³

neurophysiology defective | larval stage

Shab^unspecified

neurophysiology defective | recessive

Shab¹

neurophysiology defective | semidominant

Shab¹

paralytic | heat sensitive, with Df(3L)HR370

Shab¹

paralytic | heat sensitive (with Df(3L)M21)

Shab¹

paralytic | heat sensitive (with Shab¹)

Shab²

paralytic | heat sensitive (with Shab²)

Shab¹

paralytic | recessive | heat sensitive

paralytic | semidominant | heat sensitive

Shab¹

sleep defective, with Scer\GAL4^GMR23E10

Shab^KK111134

visible, with Scer\GAL4^pnr-MD237

Shab^GD5116

Phenotype manifest in

Allele

Shab¹

trichogen cell, with Scer\GAL4^pnr-MD237

Shab^GD5116

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (33)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

8 of 15

Yes

Yes

8 of 15

Yes

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3

3 of 15

No

Yes

1

3 of 15

No

Yes

3 of 15

No

Yes

2 of 15

No

No

2 of 15

No

No

1

2 of 15

No

No

2 of 15

No

No

4

2 of 15

No

No

2 of 15

No

Yes

2 of 15

No

Yes

1 of 15

No

No

1

1 of 15

No

No

2

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1

1 of 15

No

No

1

1 of 15

No

No

1

1 of 15

No

No

1

1 of 15

No

No

1

1 of 15

No

No

1

1 of 15

No

No

1 of 15

No

No

2

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (33)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

8 of 15

Yes

Yes

8 of 15

Yes

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

Yes

2 of 15

No

Yes

2 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Rattus norvegicus (Norway rat) (29)

5 of 13

Yes

Yes

5 of 13

Yes

Yes

3 of 13

No

Yes

3 of 13

No

Yes

3 of 13

No

Yes

3 of 13

No

Yes

3 of 13

No

Yes

3 of 13

No

Yes

3 of 13

No

Yes

2 of 13

No

No

2 of 13

No

No

2 of 13

No

Yes

2 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

Rnor\LOC100360781

1 of 13

No

No

Rnor\LOC100360841

1 of 13

No

No

Rnor\RGD1561310

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (30)

4 of 12

Yes

Yes

4 of 12

Yes

Yes

3 of 12

No

Yes

3 of 12

No

Yes

3 of 12

No

Yes

3 of 12

No

Yes

2 of 12

No

No

2 of 12

No

No

2 of 12

No

No

2 of 12

No

Yes

2 of 12

No

Yes

2 of 12

No

Yes

2 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

Yes

Xtro\LOC100494007

1 of 12

No

Yes

Xtro\LOC100497714

1 of 12

No

No

Xtro\LOC105945197

1 of 12

No

Yes

1 of 12

No

No

Danio rerio (Zebrafish) (39)

7 of 15

Yes

Yes

4 of 15

No

Yes

Drer\si:dkey-192j17.1

4 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

Drer\si:dkey-43k4.5

3 of 15

No

Yes

Drer\si:dkey-201i6.2

3 of 15

No

Yes

Drer\si:rp71-39b20.4

3 of 15

No

Yes

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

Yes

2 of 15

No

Yes

2 of 15

No

Yes

2 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Drer\LOC100004160

1 of 15

No

No

Drer\LOC100330283

1 of 15

No

No

Drer\si:ch73-334d15.4

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (14)

6 of 15

Yes

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

3 of 15

No

Yes

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (2)

1 of 15

Yes

No

1 of 15

Yes

No

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919017A )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00004358

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915054K )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY007816

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_12211

Mayetiola destructor

Hessian fly

Mdes\Mdes000073

Mayetiola destructor

Hessian fly

Mdes\Mdes000071

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009619

Anopheles darlingi

American malaria mosquito

Adar\ADAC002463

Anopheles gambiae

Malaria mosquito

Agam\AGAP005925

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ009252

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0A3H )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA011807

Danaus plexippus

Monarch butterfly

Dple\DPOGS213525-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL013678

Apis florea

Little honeybee

Aflo\2481022692

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0002bf

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002d42

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG003501

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG003502

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM520880

Rhodnius prolixus

Kissing bug

Rpro\RPRC003607

Cimex lectularius

Bed bug

Clec\CLEC012600

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008781

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_12285

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X09ZD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005391

Ixodes scapularis

Black-legged tick

Isca\ISCW000826

Stegodyphus mimosarum

African social velvet spider

Smim\KFM56525.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur28g00210

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_438

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0FP3 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\LOC100181616

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000014509

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000026161

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

KCNB1; potassium voltage-gated channel subfamily B member 1

model of early infantile epileptic encephalopathy 26

IEA

(FlyBase, 2019-)

KCNV2; potassium voltage-gated channel modifier subfamily V member 2

model of cone dystrophy

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KCNB1; potassium voltage-gated channel subfamily B member 1

8 of 15

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 26; DEE26

early infantile epileptic encephalopathy 26

KCNB2; potassium voltage-gated channel subfamily B member 2

8 of 15

KCNF1; potassium voltage-gated channel modifier subfamily F member 1

3 of 15

KCNG2; potassium voltage-gated channel modifier subfamily G member 2

3 of 15

KCNG3; potassium voltage-gated channel modifier subfamily G member 3

3 of 15

KCNS1; potassium voltage-gated channel modifier subfamily S member 1

3 of 15

KCNS2; potassium voltage-gated channel modifier subfamily S member 2

3 of 15

KCNS3; potassium voltage-gated channel modifier subfamily S member 3

3 of 15

KCNV1; potassium voltage-gated channel modifier subfamily V member 1

3 of 15

KCNV2; potassium voltage-gated channel modifier subfamily V member 2

3 of 15

RETINAL CONE DYSTROPHY 3B; RCD3B

cone dystrophy

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Sapiro et al., 2020)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Vahasoyrinki et al., 2006)

suppressible

(Vahasoyrinki et al., 2006)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Vahasoyrinki et al., 2006)

enhanceable

(Vahasoyrinki et al., 2006)

External Data

Subunit Structure (UniProtKB)

Heterotetramer of potassium channel proteins.

(UniProt, P17970 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262593

MIST (genetic) - An integrated Molecular Interaction Database

Shab

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3L

Recombination map

3-5

Cytogenetic map

Sequence location

3L:2,895,017..2,959,579 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

63A2-63A2

Limits computationally determined from genome sequence between P{PZ}Spn⁰⁶⁹¹¹ and P{PZ}l(3)06803⁰⁶⁸⁰³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

63A1-63B6

(Hegde, 1998.8.14)

63A-63A

(determined by in situ hybridisation)

(Butler et al., 1989)

Experimentally Determined Recombination Data

Location

3-5

(FlyBase, 2016)

3-6.0

(Comeron et al., 2012)

3-14

(Chopra et al., 2000)

Left of (cM)

D

(Chopra et al., 2000)

Right of (cM)

Rap1

(Chopra et al., 2000)

Notes

Stocks and Reagents

Stocks (16)

23585

w¹¹¹⁸; Mi{ET1}Shab^MB02726

85709

w¹¹¹⁸; PBac{WH}Shab^f05893

25805

y¹ v¹; P{TRiP.JF01823}attP2

34115

y¹ w^*; Mi{MIC}Shab^MI00848

42115

y¹ w^*; Mi{MIC}Shab^MI05782

43035

y¹ w^*; Mi{MIC}Shab^MI05943

43122

y¹ w^*; Mi{MIC}Shab^MI07314

43129

y¹ w^*; Mi{MIC}Shab^MI07343

v102218

P{KK111134}VIE-260B

v108861

P{KK101084}VIE-260B

v13043

w¹¹¹⁸ P{GD5116}v13043

v47632

w¹¹¹⁸; P{GD5116}v47632

More stocks available...

Genomic Clones (38)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (37)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH14308

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0262593

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0262593

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38352

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Shab CG1066

(Evangelista, 1999.11)

Source for database merge of

Source for merge of: Shab CG9965

(FlyBase Genome Annotators, 2010)

Additional comments

Annotations CG1066 and CG9965 merged as CG43128 in release 5.32 of the genome annotation.

(FlyBase Genome Annotators, 2010)

Other Comments

The role of Shab channels in Drosophila photoreceptors is to dynamically attenuate the light-induced depolarisation and prevent response saturation in bright light.

(Vahasoyrinki et al., 2006)

A null mutation in Shab reduces the voltage-activated delayed sustained current (I_K) but does not eliminate it. Observations suggest that I_K consists of two components, which are genetically, pharmacalogically and physiologically distinct. These components are I_KS (encoded by Shab) and I_KF (which defines a new voltage-activated K⁺ current).

(Singh and Singh, 1999)

Shab, Shal and Shaw account for virtually all single channel currents.

(Tsunoda and Salkoff, 1995)

Shab may encode an 11 pS slowly inactivating K⁺ channel present in embryonic neurons.

(Tsunoda and Salkoff, 1995)

Shab encodes the major delayed rectifier component of the K⁺ current in both embryonic muscles and neurons.

(Tsunoda and Salkoff, 1995)

Yeast two-hybrid system studies demonstrate the association of the hydrophilic N-terminal domains of the genes encoding channel proteins plays an important role in determining the specificity of α subunit association to form heteromultimeric potassium channels.

(Xu et al., 1995)

Expression in Xenopus oocytes reveals a delayed-rectifier type of potassium current expressed by Shab; the rate of current activation in Shab is somewhat faster than in Shaw.

(Lindsley and Zimm, 1992)

Sh, Shal, Shab and Shaw encode independent channel systems that function independently in Xenopus oocytes.

(Salkoff et al., 1992)

Shab potassium channel and a mouse brain homolog potassium channel have similar kinetic, voltage sensitive and pharmacological properties. The greatest functional difference is recovery from inactivation.

(Pak et al., 1991)

Although Sh, Shal, Shab and Shaw proteins share a conserved structral organisation, their potassium channel currents (expressed in Xenopus oocytes) differ greatly in individual kinetic properties and voltage sensitivity.

(Butler et al., 1990)

Sh, Shal, Shab and Shaw encode voltage gated potassium channels with widely varying kinetics (rate of macroscopic current activation and inactivation) and voltage sensitivity of steady state inactivation.

(Wei et al., 1990)

Potassium channel diversity could result from an extended gene family as well as from alternate splicing of the Sh primary transcript.

(Butler et al., 1989)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 74 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38352

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P17970

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0262593

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0262593

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1066

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38352

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG43128

modMine - A data warehouse for the modENCODE project

FBgn0262593

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262593

DRSC - Results frm RNAi screens

FBgn0262593

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

Shab

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG1066

(Neuert et al., 2017, Rieder et al., 2015, Tsubouchi et al., 2012, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Sepp et al., 2008)

CG43128

(Walcott et al., 2018, Hosono et al., 2015, FlyBase Genome Annotators, 2010, Ni et al., 2010.12.1)

CG9965

(Keleman et al., 2009.8.5)

K_v2

(Ping et al., 2015)

Kv2

(Yang et al., 2008, McCormack, 2003)

Shab

(Sapiro et al., 2020, Werner et al., 2020, Hill et al., 2019, Kulik et al., 2019, Smith et al., 2018, Walcott et al., 2018, Wang et al., 2018, Kim et al., 2017, Neuert et al., 2017, Pimentel et al., 2016, Berger and Crook, 2015, Carrillo et al., 2015, Fogle et al., 2015, Gene Disruption Project members, 2015-, Nagarkar-Jaiswal et al., 2015, Ashwal-Fluss et al., 2014, Frolov and Singh, 2014, Wolfram et al., 2014, Chow et al., 2013, Frolov et al., 2012, Rodriguez et al., 2012, Tsubouchi et al., 2012, Napoletano et al., 2011, Ping and Tsunoda, 2011, Diao et al., 2010, Diao et al., 2009, Frolov et al., 2008, Ryan et al., 2008, Joiner et al., 2007, Peng and Wu, 2007, Peng and Wu, 2007, Berke et al., 2006, Lee and Wu, 2006, Ueda and Wu, 2006, Vahasoyrinki et al., 2006, Bhalla et al., 2004, Wang et al., 2004, McCormack, 2003, Yao and Wu, 2001, Chopra et al., 2000)

dShab

(Carrillo et al., 2013, Hoopengardner et al., 2003)

shab

(Palu et al., 2019, Rieder et al., 2015, Bhogal et al., 2011, Jepson et al., 2011, Jepson and Reenan, 2009, Krause et al., 2008, Gasque et al., 2005, Draper et al., 2002, Young et al., 2002, Hansen et al., 1997)

shb

(Savva et al., 2012)

Name Synonyms

Shaker

(Fogle et al., 2015)

Shaker cognate b

(Tsubouchi et al., 2012, Sepp et al., 2008, Wang et al., 2004)

Secondary FlyBase IDs

FBgn0003383
FBgn0035381

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (147)