FB2021_06, released December 14, 2021

Gene: Dmel\pyd

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General Information
Symbol
Dmel\pyd
Species
D. melanogaster
Name
polychaetoid
Annotation Symbol
CG43140
Feature Type
protein_coding_gene
FlyBase ID
FBgn0262614
Gene Model Status
Current
Stock Availability
35 publicly available
Gene Summary
polychaetoid (pyd) encodes a broadly acting protein that is associated with multiple proteins at the surface and within the cytoskeleton, connecting events between the two. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
All Summaries
Also Known As

ZO-1, tamou, tam, Pyd/ZO-1

Key Links
Genomic Location
Cytogenetic map
85B2-85B3
Sequence location
Recombination map
3-48
RefSeq locus
NT_033777 REGION:8827068..8931896
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (23 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables cell adhesion molecule binding
inferred from physical interaction with FLYBASE:kirre; FB:FBgn0028369
(Weavers et al., 2009)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables cell adhesion molecule binding
inferred from biological aspect of ancestor with PANTHER:PTN001015414
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (15 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
involved_in branch fusion, open tracheal system
inferred from mutant phenotype
(Jung et al., 2006)
inferred from high throughput mutant phenotype
(Ghabrial et al., 2011)
involved_in cell-cell adhesion
inferred from mutant phenotype
(Jung et al., 2006)
involved_in chaeta development
inferred from mutant phenotype
(Djiane et al., 2011)
involved_in compound eye morphogenesis
inferred from mutant phenotype
(Seppa et al., 2008)
involved_in dorsal closure
inferred from mutant phenotype
(Choi et al., 2011)
involved_in fusion cell fate specification
inferred from mutant phenotype
(Jung et al., 2006)
involved_in head involution
inferred from mutant phenotype
(Choi et al., 2011)
involved_in negative regulation of endocytosis
inferred from mutant phenotype
(Shimizu et al., 2017)
involved_in nephrocyte diaphragm assembly
inferred from mutant phenotype
(Carrasco-Rando et al., 2019, Zhuang et al., 2009)
involved_in open tracheal system development
inferred from mutant phenotype
(Choi et al., 2011)
involved_in regulation of cell adhesion
inferred from mutant phenotype
(Seppa et al., 2008)
inferred from genetic interaction with FLYBASE:rst; FB:FBgn0003285
(Seppa et al., 2008)
inferred from genetic interaction with FLYBASE:shg; FB:FBgn0003391
(Seppa et al., 2008)
involved_in regulation of embryonic cell shape
inferred from mutant phenotype
(Choi et al., 2011)
involved_in wing disc development
inferred from mutant phenotype
(Djiane et al., 2011)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in cell-cell adhesion
inferred from biological aspect of ancestor with PANTHER:PTN001015414
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in cell-cell junction organization
inferred from biological aspect of ancestor with PANTHER:PTN001015414
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in protein localization to cell-cell junction
inferred from biological aspect of ancestor with PANTHER:PTN001015414
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (7 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
located_in adherens junction
inferred from direct assay
(Djiane et al., 2011, Seppa et al., 2008, Jung et al., 2006)
located_in apical part of cell
inferred from direct assay
(Seppa et al., 2008)
located_in axon
inferred from direct assay
(Seppa et al., 2008)
located_in basolateral plasma membrane
inferred from direct assay
(Seppa et al., 2008)
located_in nephrocyte diaphragm
inferred from direct assay
(Carrasco-Rando et al., 2019, Weavers et al., 2009)
located_in plasma membrane
inferred from direct assay
(Weavers et al., 2009)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in bicellular tight junction
inferred from biological aspect of ancestor with PANTHER:PTN001015414
(assigned by GO_Central )
(Gaudet et al., 2011)
is_active_in plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN001015414
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
polychaetoid (pyd) encodes a broadly acting protein that is associated with multiple proteins at the surface and within the cytoskeleton, connecting events between the two. [Date last reviewed: 2019-03-14]
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
pyd: polychaetoid
Extra bristles present in homozygote at or near almost all normal bristle locations but most frequently in dorsocentral and scutellar regions. Heterozygote in some stocks occasionally shows extra bristles, especially vibrissae. Character expressed better at low temperatures and in large flies. Combinations with h and Hw generally superadditive for bristle number. RK3.
Summary (Interactive Fly)

scaffolding protein, PDZ domain protein, MAGUK - involved in sensory organ development - mutations have a neurogenic effect - suppresses Deltex-regulated Notch activity to modulate germline stem cell niche formation

Gene Model and Products
Number of Transcripts
12
Number of Unique Polypeptides
11

Please see the JBrowse view of Dmel\pyd for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.42

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.47

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 6.31

Sequence Ontology: Class of Gene
gene_with_edited_transcript
(Rodriguez et al., 2012, Graveley et al., 2011)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
pyd-RA
FBtr0305297
NM_169245
7195
2090
pyd-RB
FBtr0305295
NM_057349
5688
1371
pyd-RC
FBtr0305296
NM_169246
5024
1293
pyd-RF
FBtr0305294
NM_176426
4196
1149
pyd-RG
FBtr0305299
NM_001104241
5048
1301
pyd-RI
FBtr0305298
NM_001144556
5505
1386
pyd-RJ
FBtr0305301
NM_170646
6665
1735
pyd-RK
FBtr0305302
NM_001104242
5744
1446
pyd-RL
FBtr0305303
NM_001260071
5123
1285
pyd-RM
FBtr0305304
NM_001260072
6058
1301
pyd-RO
FBtr0310205
NM_001260069
8533
2395
pyd-RP
FBtr0479647
NM_001382097
5408
1390
Additional Transcript Data and Comments
Reported size (kB)

10.5, 9.5, 8.1, 6.7, 5.6 (northern blot)

(Takahisa et al., 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
pyd-PA
FBpp0293840
230.2
2090
8.24
NP_731291
AAF54301
pyd-PB
FBpp0293838
148.8
1371
9.47
NP_476697
AAF54300
pyd-PC
FBpp0293839
140.5
1293
9.78
NP_731292
AAN13403
pyd-PF
FBpp0293837
125.6
1149
9.10
NP_788603
AAN13402
pyd-PG
FBpp0293842
142.1
1301
9.76
NP_001097711
ABW08622
pyd-PI
FBpp0293841
150.4
1386
9.43
NP_001138028
ACL83487
pyd-PJ
FBpp0293844
187.9
1735
8.91
NP_731293
AAF54303
pyd-PK
FBpp0293845
159.5
1446
9.15
NP_001097712
ABW08623
pyd-PL
FBpp0293846
141.0
1285
9.39
NP_001247000
AFH06318
pyd-PM
FBpp0293847
142.1
1301
9.76
NP_001247001
AFH06319
pyd-PO
FBpp0301889
263.8
2395
8.81
NP_001246998
AFH06316
pyd-PP
FBpp0424324
153.0
1390
9.82
AFH06317
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1301 aa isoforms: pyd-PG, pyd-PM
Additional Polypeptide Data and Comments
Reported size (kDa)

180 (kD observed)

(Wei and Ellis, 2001)

1367 (aa); 170, 160 (kD observed); 148 (kD predicted)

(Takahisa et al., 1996)
Comments

Antisera specific to the the larger pyd protein was prepared.

(Wei and Ellis, 2001)

Cortactin protein SH3 domain binds to a PXXP motif in the pyd protein proline-rich domain. This binding is abolished when the central 2 P residues in a motif consisting of 3 overlapping PXXP motifs are mutated to A. Cortactin and pyd proteins coimmunoprecipitate from Drosophila embryo lysates.

(Katsube et al., 1998)

tam protein contains three discs-large homologous regions, an SH3 domain, a guanylate kinase domain and a proline rich terminal region. It is a structural homolog of mammalian ZO-1.

(Takahisa et al., 1996)
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pyd using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal ectoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
hindgut anlage
(Fisher et al., 2012)
mesectoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)
embryonic stage 9 -- 10
dorsal ectoderm
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 10 -- 11
embryonic/larval trachea
(Chung et al., 2011)
embryonic stage 11 -- 12
clypeo-labral primordium
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
tracheal primordium
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
atrium
(Fisher et al., 2012)
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic epipharynx
(Fisher et al., 2012)
embryonic esophagus
(Fisher et al., 2012)
embryonic foregut
(Fisher et al., 2012)
embryonic head epidermis
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic hypopharynx
(Fisher et al., 2012)
embryonic ventral apodeme
(Fisher et al., 2012)
embryonic ventral epidermis
(Fisher et al., 2012)
embryonic/larval garland cell
(Fisher et al., 2012)
ventral midline of embryo
(Fisher et al., 2012)
third instar larval stage
wing disc
(Takahisa et al., 1996)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
(Takahisa et al., 1996)
larval stage & pupal stage
(Takahisa et al., 1996)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
(Wei and Ellis, 2001)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Wei and Ellis, 2001)
embryonic stage 12 -- 17
nephrocyte
(Carrasco-Rando et al., 2019)
larval stage
garland cell
(Tutor et al., 2014)
nephrocyte
(Carrasco-Rando et al., 2019)
larval stage & pupal stage
wing disc
(Wei and Ellis, 2001)
third instar larval stage
wing disc
(Takahisa et al., 1996)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data

tam protein is localized at cell-cell junctions in a position slightly basal to shg.

(Carrasco-Rando et al., 2019, Takahisa et al., 1996)

The large form of pyd protein was assayed in embryos and wing discs and is present in both.

(Wei and Ellis, 2001, Katsube et al., 1998)

The larger form of pyd protein is localized at the adherens junction.

(Wei and Ellis, 2001)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in adherens junction
inferred from direct assay
(Djiane et al., 2011, Seppa et al., 2008, Jung et al., 2006)
located_in apical part of cell
inferred from direct assay
(Seppa et al., 2008)
located_in axon
inferred from direct assay
(Seppa et al., 2008)
located_in basolateral plasma membrane
inferred from direct assay
(Seppa et al., 2008)
located_in nephrocyte diaphragm
inferred from direct assay
(Carrasco-Rando et al., 2019, Weavers et al., 2009)
located_in plasma membrane
inferred from direct assay
(Weavers et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\pyd in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 67 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 34 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of pyd
Transgenic constructs containing regulatory region of pyd
Aberrations (Deficiencies and Duplications) ( 7 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | recessive
partially lethal - majority die
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable | maternal effect
viable | maternal effect (with Df(3R)p-XT103)
Sterility
Allele
female fertile
fertile
male fertile
Other Phenotypes
Allele
abnormal behavior | recessive
abnormal cell migration | pupal stage, with Scer\GAL4GMR.PF
abnormal cell shape
abnormal learning | adult stage
increased cell number (with Df(3R)p-XT103)
increased cell number | adult stage, with Scer\GAL4GMR.PU
increased cell number | pupal stage, with Scer\GAL4GMR.PF
majority die during embryonic stage (with pydB12)
majority die during embryonic stage (with pydex147)
some die during embryonic stage
some die during embryonic stage (with pydB12)
some die during embryonic stage (with pydex147)
some die during embryonic stage (with pydex180)
visible
visible, with Scer\GAL4pnr-MD237
visible (with Df(3R)p-XT103)
visible (with pydC5)
visible (with pydex147)
visible (with pydtam)
visible | adult stage, with Scer\GAL4ap.PU
visible | adult stage, with Scer\GAL4GMR.PF
visible | recessive
wild-type
Phenotype manifest in
Allele
adult abdominal sensillum
adult thorax
adult thorax, with Scer\GAL4GMR.PF
basement membrane | embryonic stage 16
cephalopharyngeal skeleton
chaeta
chaeta, with Scer\GAL4GMR.PF
chaeta, with Scer\GAL4pnr-MD237
cone cell
cone cell | increased number, with Scer\GAL4GMR.PU
cone cell | pupal stage, with Scer\GAL4GMR.PF
denticle belt
dorsal branch primordium
dorsal branch primordium | somatic clone
dorsocentral bristle
dorsocentral bristle | increased number (with Df(3R)p-XT103)
dorsocentral bristle | increased number (with pydC5)
dorsocentral bristle | increased number (with pydtam)
embryo | dorsal closure stage
embryo | dorsal closure stage (with pydB12)
embryo | dorsal closure stage (with pydex147)
embryo | dorsal closure stage (with pydex180)
embryonic/first instar larval cuticle
embryonic/larval cuticle
embryonic/larval dorsal branch
embryonic/larval dorsal trunk
embryonic/larval garland cell, with Scer\GAL4sns.GCN
embryonic/larval lateral trunk
embryonic/larval tracheal system
embryonic dorsal branch & cell-cell adherens junction
embryonic dorsal branch & cell-cell adherens junction | somatic clone
embryonic ganglionic branch & cell-cell adherens junction
embryonic garland cell
embryonic garland cell | embryonic stage 16
embryonic head
embryonic head (with pydB12)
embryonic head (with pydex147)
embryonic head (with pydex180)
embryonic leading edge cell
embryonic leading edge cell (with pydB12)
embryonic leading edge cell (with pydex147)
embryonic leading edge cell (with pydex180)
eye
eye, with Scer\GAL4GMR.PF
eye | adult stage, with Scer\GAL4GMR.PF
eye | somatic clone
female germline stem cell | increased number (with Df(3R)p-XT103)
ganglionic branch primordium
germarium cap cell | increased number
germarium cap cell | increased number (with Df(3R)p-XT103)
interocellar bristle
interommatidial cell | increased number, with Scer\GAL4GMR.PU
interommatidial precursor cell | pupal stage
interommatidial precursor cell | pupal stage | increased number
interommatidial precursor cell | pupal stage | increased number, with Scer\GAL4GMR.PF
lateral trunk primordium
macrochaeta
macrochaeta, with Scer\GAL4ap.PU
macrochaeta & adult head | dorsal
macrochaeta & adult thorax | dorsal
macrochaeta | increased number
macrochaeta | increased number, with Scer\GAL4ap.PU
mesothoracic metatarsus
mesothoracic tarsal bristle
microchaeta, with Scer\GAL4ap.PU
ommatidium
ommatidium, with Scer\GAL4GMR.PU
orbital bristle
presumptive embryonic/larval tracheal system
presumptive embryonic/larval tracheal system (with Df(3R)p-XT103)
primary pigment cell
primary pigment cell | pupal stage, with Scer\GAL4GMR.PF
scutellar bristle
secondary pigment cell
sensory mother cell
septate junction | ectopic | embryonic stage 16
slit diaphragm | embryonic stage 16
sternopleural bristle
terminal tracheal cell
tertiary pigment cell | pupal stage | increased number
tertiary pigment cell | pupal stage | increased number, with Scer\GAL4GMR.PF
thorax
thorax, with Scer\GAL4ap.PU
tracheal fusion cell
tracheal fusion cell | somatic clone
trichogen cell, with Scer\GAL4pnr-MD237
ventral head epidermis
ventral head epidermis (with pydB12)
ventral head epidermis (with pydex147)
ventral head epidermis (with pydex180)
vertical bristle
wing (with Df(3R)p-XT103)
wing (with pydex147)
wing (with pydtam)
wing sensillum
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\TJP2
13 of 15
Yes
Yes
Hsap\TJP1
11 of 15
No
Yes
Hsap\TJP3
11 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Tjp2
13 of 15
Yes
Yes
Mmus\Tjp3
11 of 15
No
Yes
Mmus\Tjp1
10 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
Rnor\Tjp1
10 of 13
Yes
Yes
Rnor\Tjp2
9 of 13
No
Yes
Rnor\Tjp3
9 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (4)
Xtro\tjp2
7 of 12
Yes
Yes
Xtro\tjp3
7 of 12
Yes
Yes
Xtro\tjp1
6 of 12
No
Yes
Xtro\MGC76064
1 of 12
No
Yes
Danio rerio (Zebrafish) (6)
Drer\tjp2a
12 of 15
Yes
Yes
Drer\tjp1a
7 of 15
No
Yes
Drer\tjp1b
6 of 15
No
Yes
Drer\tjp3
5 of 15
No
Yes
Drer\tjp2b
4 of 15
No
Yes
Drer\LOC103911473
2 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\zoo-1
12 of 15
Yes
Yes
Cele\magu-4
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919006K )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00009498
Drosophila simulans
Dsim\GD20836
Drosophila sechellia
Dsec\GM26299
Drosophila erecta
Dere\GG17413
Drosophila yakuba
Dyak\GE24813
Drosophila ananassae
Dana\GF17278
Drosophila pseudoobscura pseudoobscura
Dpse\GA30196
Drosophila persimilis
Dper\GL22280
Drosophila persimilis
Dper\GL22283
Drosophila willistoni
Dwil\GK11118
Drosophila virilis
Dvir\GJ23434
Drosophila virilis
Dvir\GJ26529
Drosophila mojavensis
Dmoj\GI22708
Drosophila mojavensis
Dmoj\GI22704
Drosophila grimshawi
Dgri\GH22315
Drosophila grimshawi
Dgri\GH22524
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915003E )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17011612
Musca domestica
House fly
Mdoa\17026067
Glossina morsitans
Tsetse fly
Gmor\GMOY008067
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_12728
Mayetiola destructor
Hessian fly
Mdes\Mdes001990
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL018313
Anopheles darlingi
American malaria mosquito
Adar\ADAC004024
Anopheles gambiae
Malaria mosquito
Agam\AGAP003546
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ007474
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00ME )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA010279
Danaus plexippus
Monarch butterfly
Dple\DPOGS207354-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS207353-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS207352-PA
Apis florea
Little honeybee
Aflo\2856373092
Apis mellifera
Western honey bee
Amel\GB42401
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP11949
Bombus terrestris
Buff-tailed bumblebee
Bter\15216871
Linepithema humile
Argentine ant
Lhum\LH11977
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001c7b
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000464
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_12771
Tribolium castaneum
Red flour beetle
Tcas\TC006063
Pediculus humanus
Human body louse
Phum\PHUM359090
Rhodnius prolixus
Kissing bug
Rpro\RPRC005715
Rhodnius prolixus
Kissing bug
Rpro\RPRC005706
Cimex lectularius
Bed bug
Clec\CLEC000909
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI002448
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_01360
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06VG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR009888
Ixodes scapularis
Black-legged tick
Isca\ISCW019818
Stegodyphus mimosarum
African social velvet spider
Smim\KFM74781.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM71564.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur33g01420
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_308707
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0AJZ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Tjp1
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000008103
Ciona intestinalis
Vase tunicate
Cint\LOC100185856
Gallus gallus
Domestic chicken
Ggal\TJP1
Gallus gallus
Domestic chicken
Ggal\TJP2
Gallus gallus
Domestic chicken
Ggal\TJP3
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Dlg5
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Interaction
    References
    pydHMS00263
    exacerbates  atypical teratoid rhabdoid tumor
    modeled by Snr1KK101602
    (Tegeder et al., 2019)
    pydEY01326
    exacerbates  tauopathy
    modeled by Hsap\MAPTUAS.cWa
    (Feuillette et al., 2020)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    TJP2; tight junction protein 2
    13 of 15
    607709
    TJP1; tight junction protein 1
    11 of 15
    601009
        TJP3; tight junction protein 3
        11 of 15
        612689
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser
            View in Interactions Browser
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            pyd - Cortactin
            two hybrid, anti bait coimmunoprecipitation, western blot
            (Katsube et al., 1998)
            pyd - N
            anti tag coimmunoprecipitation, anti tag western blot
            (Shimizu et al., 2017)
            pyd - Nedd4
            two hybrid
            (Djiane et al., 2011)
            pyd - Nrg
            filter binding
            (Goossens et al., 2011)
            pyd - Su(dx)
            anti tag coimmunoprecipitation, western blot, pull down, autoradiography, colocalization, fluorescence microscopy, inferred by author, two hybrid, classical fluorescence spectroscopy
            (Djiane et al., 2011)
            pyd - Wee1
            anti tag coimmunoprecipitation, anti tag western blot
            (Sopko et al., 2014)
            pyd - cno
            pull down, autoradiography, two hybrid
            (Takahashi et al., 1998)
            pyd - drk
            anti tag coimmunoprecipitation, peptide massfingerprinting
            (Friedman et al., 2011)
            pyd - dx
            anti tag coimmunoprecipitation, anti tag western blot
            (Shimizu et al., 2017)
            pyd - kel
            proximity-dependent biotin identification, Identification by mass spectrometry
            (Mannix et al., 2019)
            pyd - kirre
            anti tag coimmunoprecipitation, anti tag western blot, two hybrid
            (Weavers et al., 2009)
            pyd - sdk
            pull down, anti tag western blot
            (Letizia et al., 2019)
            RNA-protein
            Physical Interaction
            Assay
            References
            pyd - CG44249
            anti tag coimmunoprecipitation, reverse transcription pcr
            (Li et al., 2020)
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            pyd
            enhanceable
            Dl
            (Chen et al., 1996)
            pyd
            enhanceable
            emc
            (Chen et al., 1996)
            pyd
            enhanceable
            ena
            (Choi et al., 2011)
            pyd
            enhanceable
            ex
            (Djiane et al., 2011)
            pyd
            enhanceable
            h
            (Held, 1990)
            pyd
            suppressible
            N
            (Djiane et al., 2011)
            pyd
            enhanceable
            N
            (Djiane et al., 2011)
            pyd
            enhanceable
            rst
            (Seppa et al., 2008)
            pyd
            enhanceable
            shg
            (Seppa et al., 2008)
            pyd
            suppressible
            Su(dx)
            (Djiane et al., 2011)
            pyd
            enhanceable
            tkv
            (Seppa et al., 2008)
            pyd
            enhanceable
            α-Cat
            (Seppa et al., 2008)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            ena
            enhanceable
            pyd
            (Choi et al., 2011)
            h
            enhanceable
            pyd
            (Held, 1990)
            hep
            enhanceable
            pyd
            (Takahashi et al., 1998)
            N
            suppressible
            pyd
            (Chen et al., 1996)
            Su(dx)
            enhanceable
            pyd
            (Djiane et al., 2011)
            tkv
            enhanceable
            pyd
            (Seppa et al., 2008)
            External Data
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Linkouts
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3R
            Recombination map
            3-48
            Cytogenetic map
            85B2-85B3
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            85B2-85B3
            Limits computationally determined from genome sequence between P{lacW}l(3)L4740L4740 and P{EP}D1EP473
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            85B1-85B6
            (Takahisa, 1996.2.7)
            Located in 85B1--85B6 by deficiency mapping (details unspecified).
            (Takahisa et al., 1996)
            85B1-85B6
            85B (determined by in situ hybridisation)
            (Takahisa et al., 1996)
            85B9-85B9
            (determined by in situ hybridisation)
            (Chen et al., 1996)
            Experimentally Determined Recombination Data
            Location

            3-48

            (FlyBase, 2016)

            3-45.5

            (Comeron et al., 2012)

            3-

            (Takahisa, 1996.2.7)

            3-48.1

            (Ellis et al., 1995)
            Left of (cM)
            Right of (cM)
            Notes

            Maps to 3R.

            (Ghabrial et al., 2011)
            Stocks and Reagents
            Stocks (35)
            Genomic Clones (45)
            cDNA Clones (129)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Carrasco-Rando et al., 2019, Djiane et al., 2011, Seppa et al., 2008, Wei and Ellis, 2001, Takahisa et al., 1996)

            monoclonal

            (Choi et al., 2011)
            Commercially Available Antibodies
             
            Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
            Other Information
            Relationship to Other Genes
            Source for database identify of
            Source for database merge of

            Source for merge of: pyd tam

            (Ellis, 1998.6.17)

            Source for merge of: pyd CG11782 CG11962 CG12409 CG9729

            (FlyBase Genome Annotators, 2002-2003)

            Source for merge of: pyd CG9731

            (FlyBase Genome Annotators, 2011)
            Additional comments

            Annotations CG31349 and CG9731 merged as CG43140 in release 5.33 of the genome annotation.

            (FlyBase Genome Annotators, 2011)

            Annotations CG9763, CG11782, CG11962, CG12409 and CG9729 merged as CG31349 in release 3 of the genome annotation.

            (FlyBase Genome Annotators, 2002-2003)
            Other Comments

            Most embryos lacking pyd die with striking defects in morphogenesis of embryonic epithelia including the epidermis, segmental grooves, and tracheal system.

            (Choi et al., 2011)

            Loss of maternal and zygotic pyd substantially compromises development.

            (Choi et al., 2011)

            ?? need to add more comments and GO terms

            (Choi et al., 2011)

            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

            (Kiger et al., 2003)

            Candidate gene for quantitative trait (QTL) locus determining bristle number.

            (Nuzhdin et al., 1999)

            cno protein binds directly to pyd protein.

            (Takahashi et al., 1998)

            The junction associated pyd protein is involved in the structural organisation or regulation of the putative components important for a signaling pathway to activate the emc expression.

            (Takahisa et al., 1996)

            "3-48.1" was stated as revision.

            (Ellis et al., 1995)

            In mutants of pyd inappropriate cell fate choices are made resulting in the presence of ectopic sensory organs. A decrease in pyd function results in defects in the process of cell to cell communication that normally restricts the sensory organ precursor to a single cell in each proneural cluster.

            (Ellis et al., 1995)

            Recessive P-element insert alleles have extra macrochaetae on head and thorax. Acts as a dominant enhancer of emc.

            Origin and Etymology
            Discoverer

            Spencer, 31st Aug. 1939.

            Etymology

            'tam' means 'hairy' in Japanese.

            (Takahisa et al., 1996)
            Identification
            External Crossreferences and Linkouts ( 120 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
            Linkouts
            ApoDroso - Functional genomic database for photoreceptor development, survival and function
            DroID - A comprehensive database of gene and protein interactions.
            Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Synonyms and Secondary IDs (26)
            Reported As
            Symbol Synonym
            CG11782
            CG11962
            CG12409
            CG31349
            (Rosa-Ferreira et al., 2015, Christis and Munro, 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Deloger et al., 2009, Hazelett et al., 2009, Perkins et al., 2009, Sepp et al., 2008, Maisonhaute et al., 2007)
            CG43140
            (FlyBase Genome Annotators, 2011, Ni et al., 2010.12.1)
            CG9729
            CG9731
            (Keleman et al., 2009.8.5)
            CG9763
            (Norga et al., 2003)
            CT36877
            (Ceriani et al., 2002)
            DZO-1
            (Katsube et al., 1998)
            Pch
            Pyd
            (Wen et al., 2020, Dlugos et al., 2019, Helmstädter and Simons, 2017, Chandra et al., 2015, Flores-Benitez and Knust, 2015, Sopko et al., 2014, Tutor et al., 2014, Guichard et al., 2013, Choi et al., 2011, Höhne et al., 2011, Ivarsson et al., 2011, Waldholm et al., 2011, Weavers et al., 2009, Gabut et al., 2007, Jennings et al., 2007, Wu et al., 2007, Wei and Ellis, 2001)
            Pyd/ZO-1
            (Jung et al., 2006, Müller and Bossinger, 2003, Tepass et al., 2001)
            Tamou
            (Caruana, 2002, Campuzano, 2001)
            ZO-1
            (Mysh and Poulton, 2021, Yu and Guan, 2013, Bulgakova et al., 2012, Bakal et al., 2008, Anderson et al., 2004, Martin and Parkhurst, 2004, Roper and Brown, 2003, Knox and Brown, 2002, Bilder, 2001, Peifer and Tepass, 2000, Noselli and Agnes, 1999, Stronach and Perrimon, 1999, Ueda et al., 1999, Takahashi et al., 1998)
            ZO1
            (Jacinto et al., 2002)
            dzo-1
            (Yeaman et al., 1999)
            polychaetoid
            (Staudt et al., 2005)
            pyd
            (Michki et al., 2021, Parkhitko et al., 2021, Feuillette et al., 2020, Geigges et al., 2020, Li et al., 2020, Overton et al., 2020, Carrasco-Rando et al., 2019, Donde et al., 2019, Harbison et al., 2019, Letizia et al., 2019, Mannix et al., 2019, Meltzer et al., 2019, Ruiz, 2019.12.30, Tegeder et al., 2019, Millet-Boureima et al., 2018, Hermle et al., 2017, Rohde et al., 2017, Shimizu et al., 2017, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Piegholdt et al., 2016, Aradska et al., 2015, Chaston et al., 2015, Gene Disruption Project members, 2015-, Kavi et al., 2015, Matthews et al., 2015, Na et al., 2015, Zaessinger et al., 2015, Ashwal-Fluss et al., 2014, Chaturvedi et al., 2014, Ciglar et al., 2014, Xu et al., 2014, Carter, 2013, Das et al., 2013, Zhang et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Rodriguez et al., 2012, Choi et al., 2011, Chung et al., 2011, Djiane et al., 2011, Friedman et al., 2011, Goossens et al., 2011, Cook et al., 2010.2.12, Kong et al., 2010, Magwire et al., 2010, Flaherty et al., 2009, Weavers et al., 2009, Zhuang et al., 2009, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Font-Burgada et al., 2008, Seppa et al., 2008, Akdemir et al., 2007, Araujo et al., 2007, Kankel et al., 2007, Maisonhaute et al., 2007, Weiler, 2007, Yi et al., 2007, Jung et al., 2006, Held, 1990)
            pyd(Z01)
            (Brumby et al., 2004)
            tam
            (Ponting et al., 1997, Ueda et al., 1997, Takahisa, 1996.2.7, Takahisa et al., 1996)
            xvt
            Name Synonyms
            Polychaetoid
            (Finegan and Bergstralh, 2020, Yamagata, 2020, Guichard et al., 2013, Choi et al., 2011, Rodrigues et al., 2011, Chang et al., 2008, Araujo et al., 2007, Gabut et al., 2007, Jennings et al., 2007)
            extra verticals
            failed fusions
            (Ghabrial et al., 2011)
            polychaetoid
            (Das et al., 2013, Eddison et al., 2012, Cammarato et al., 2011, FlyBase Genome Annotators, 2011, Ivarsson et al., 2011, Flaherty et al., 2009, Hazelett et al., 2009, Seppa et al., 2008, Sepp et al., 2008, Kankel et al., 2007, Jung et al., 2006, Burnette et al., 2005, Staudt et al., 2005)
            tamou
            (Dimitratos et al., 1999, Ponting et al., 1997, Ueda et al., 1997, Takahisa, 1996.2.7, Takahisa et al., 1996)
            Secondary FlyBase IDs
            • FBgn0003177
            • FBgn0010330
            • FBgn0037639
            • FBgn0037641
            • FBgn0037642
            • FBgn0040533
            • FBgn0051349
            • FBgn0037640
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (216)