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General Information
Symbol
Dmel\pyd
Species
D. melanogaster
Name
polychaetoid
Annotation Symbol
CG43140
Feature Type
FlyBase ID
FBgn0262614
Gene Model Status
Stock Availability
Gene Snapshot
polychaetoid (pyd) encodes a broadly acting protein that is associated with multiple proteins at the surface and within the cytoskeleton, connecting events between the two. [Date last reviewed: 2019-03-14]
Also Known As
ZO-1, tamou, tam, Pyd/ZO-1
Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:8,827,068..8,931,896 [-]
Recombination map
3-48
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
Predictions / Assertions
-
Summaries
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
pyd: polychaetoid
Extra bristles present in homozygote at or near almost all normal bristle locations but most frequently in dorsocentral and scutellar regions. Heterozygote in some stocks occasionally shows extra bristles, especially vibrissae. Character expressed better at low temperatures and in large flies. Combinations with h and Hw generally superadditive for bristle number. RK3.
Summary (Interactive Fly)
scaffolding protein, PDZ domain protein, MAGUK - involved in sensory organ development - mutations have a neurogenic effect - suppresses Deltex-regulated Notch activity to modulate germline stem cell niche formation
Gene Model and Products
Number of Transcripts
11
Number of Unique Polypeptides
10

Please see the GBrowse view of Dmel\pyd or the JBrowse view of Dmel\pyd for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.42
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.47
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0305297
7195
2090
FBtr0305295
5688
1371
FBtr0305296
5024
1293
FBtr0305294
4196
1149
FBtr0305299
5048
1301
FBtr0305298
5505
1386
FBtr0305301
6665
1735
FBtr0305302
5744
1446
FBtr0305303
5123
1285
FBtr0305304
6058
1301
FBtr0310205
8533
2395
Additional Transcript Data and Comments
Reported size (kB)
10.5, 9.5, 8.1, 6.7, 5.6 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0293840
230.2
2090
8.24
FBpp0293838
148.8
1371
9.47
FBpp0293839
140.5
1293
9.78
FBpp0293837
125.6
1149
9.10
FBpp0293842
142.1
1301
9.76
FBpp0293841
150.4
1386
9.43
FBpp0293844
187.9
1735
8.91
FBpp0293845
159.5
1446
9.15
FBpp0293846
141.0
1285
9.39
FBpp0293847
142.1
1301
9.76
FBpp0301889
263.8
2395
8.81
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1301 aa isoforms: pyd-PG, pyd-PM
Additional Polypeptide Data and Comments
Reported size (kDa)
180 (kD observed)
1367 (aa); 170, 160 (kD observed); 148 (kD predicted)
Comments
Antisera specific to the the larger pyd protein was prepared.
Cortactin protein SH3 domain binds to a PXXP motif in the pyd protein proline-rich domain. This binding is abolished when the central 2 P residues in a motif consisting of 3 overlapping PXXP motifs are mutated to A. Cortactin and pyd proteins coimmunoprecipitate from Drosophila embryo lysates.
tam protein contains three discs-large homologous regions, an SH3 domain, a guanylate kinase domain and a proline rich terminal region. It is a structural homolog of mammalian ZO-1.
External Data
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pyd using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (23 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (0 terms)
Biological Process (15 terms)
Terms Based on Experimental Evidence (15 terms)
CV Term
Evidence
References
inferred from high throughput mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:dx; FB:FBgn0000524
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:rst; FB:FBgn0003285
inferred from genetic interaction with FLYBASE:shg; FB:FBgn0003391
inferred from mutant phenotype
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (7 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
The large form of pyd protein was assayed in embryos and wing discs and is present in both.
The larger form of pyd protein is localized at the adherens junction.
Cortactin protein colocalizes with pyd protein to intercellular junctions of imaginal disc epithelial cells.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\pyd in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 67 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 32 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of pyd
Transgenic constructs containing regulatory region of pyd
Deletions and Duplications ( 7 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
embryonic dorsal branch & cell-cell adherens junction
embryonic dorsal branch & cell-cell adherens junction | somatic clone
embryonic ganglionic branch & cell-cell adherens junction
macrochaeta & adult head | dorsal
macrochaeta & adult thorax | dorsal
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
10 of 15
Yes
Yes
7 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
9 of 15
Yes
Yes
8 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (7)
9 of 13
Yes
Yes
6 of 13
No
Yes
5 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (6)
4 of 12
Yes
Yes
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (11)
9 of 15
Yes
Yes
6 of 15
No
Yes
4 of 15
No
Yes
3 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
9 of 15
Yes
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (1)
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (1)
1 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919006K )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila virilis
Drosophila mojavensis
Drosophila mojavensis
Drosophila grimshawi
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915003E )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00ME )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06VG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0AJZ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (8)
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    enhanceable
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    enhanceable
    suppressible
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-48
    Cytogenetic map
    Sequence location
    3R:8,827,068..8,931,896 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    85B3-85B7
    Limits computationally determined from genome sequence between P{lacW}l(3)L4740L4740 and P{EP}D1EP473
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Located in 85B1--85B6 by deficiency mapping (details unspecified).
    85B1-85B6
    85B (determined by in situ hybridisation)
    85B9-85B9
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (43)
    Genomic Clones (45)
    cDNA Clones (129)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of
    Source for merge of: pyd tam
    Source for merge of: pyd CG11782 CG11962 CG12409 CG9729
    Source for merge of: pyd CG9731
    Additional comments
    Annotations CG31349 and CG9731 merged as CG43140 in release 5.33 of the genome annotation.
    Annotations CG9763, CG11782, CG11962, CG12409 and CG9729 merged as CG31349 in release 3 of the genome annotation.
    Other Comments
    Most embryos lacking pyd die with striking defects in morphogenesis of embryonic epithelia including the epidermis, segmental grooves, and tracheal system.
    Loss of maternal and zygotic pyd substantially compromises development.
    ?? need to add more comments and GO terms
    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
    Candidate gene for quantitative trait (QTL) locus determining bristle number.
    cno protein binds directly to pyd protein.
    The junction associated pyd protein is involved in the structural organisation or regulation of the putative components important for a signaling pathway to activate the emc expression.
    "3-48.1" was stated as revision.
    In mutants of pyd inappropriate cell fate choices are made resulting in the presence of ectopic sensory organs. A decrease in pyd function results in defects in the process of cell to cell communication that normally restricts the sensory organ precursor to a single cell in each proneural cluster.
    Recessive P-element insert alleles have extra macrochaetae on head and thorax. Acts as a dominant enhancer of emc.
    Origin and Etymology
    Discoverer
    Spencer, 31st Aug. 1939.
    Etymology
    'tam' means 'hairy' in Japanese.
    Identification
    External Crossreferences and Linkouts ( 112 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    ApoDroso - Functional genomic database for photoreceptor development, survival and function
    DroID - A comprehensive database of gene and protein interactions.
    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (26)
    Reported As
    Symbol Synonym
    CG11782
    CG11962
    CG12409
    CG9729
    Pch
    polychaetoid
    xvt
    Secondary FlyBase IDs
    • FBgn0003177
    • FBgn0010330
    • FBgn0037639
    • FBgn0037641
    • FBgn0037642
    • FBgn0040533
    • FBgn0051349
    • FBgn0037640
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (199)