Gene Dmel\Sap130

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General Information

Symbol

Dmel\Sap130

Species

D. melanogaster

Name

Sin3A-associated protein 130

Annotation symbol

CG11006

Feature type

protein_coding_gene

FlyBase ID

FBgn0262714

Gene Model Status

Current

Stock availability

5 publicly available

Also Known As

BcDNA:LD21213, SAP130

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

69E3-69E4

Sequence location

3L:12,798,993..12,806,136 [+]

Genomic Maps

Select View:

modENCODE GBrowse

Summary Information

Automatically generated summary

See sections below for more information

The gene Sin3A-associated protein 130 is referred to in FlyBase by the symbol Dmel\Sap130 (CG11006, FBgn0262714). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: mitotic spindle organization. 6 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Histone deacetylase complex subunit SAP130. Gene sequence location is 3L:12798993..12806136.

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2011_10

References

Knowles-Barley, 2011.8.24, BrainTrap expression curation.

FB2012_01

Sequence features

BKN21860

HFA09680

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

69E3-69E4

Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

69E3-69E4

(determined by in situ hybridisation)

(Spradling et al., 1999)

69E3-69E4

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Sap130 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Sap130-RA

FBtr0075927

NM_140345

4054

1219

Sap130-RB

FBtr0075928

NM_168518

4168

1257

Sap130-RC

FBtr0075929

NM_168517

4279

1294

Sap130-RE

FBtr0114506

NM_168519

4060

1257

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Sap130-PA

FBpp0075659

123.6

1219

11.28

NP_648602

AAF49877

Sap130-PB

FBpp0075660

127.5

1257

11.29

NP_729835

AAN11859

Sap130-PC

FBpp0075661

131.4

1294

11.31

NP_729834

AAN11858

Sap130-PE

FBpp0112998

127.5

1257

11.29

NP_729836

AAN11860

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Histone deacetylase complex subunit SAP130 (IPR024137)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Notes

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Notes

High-Throughput Expression Data

Untitled Document detailed view

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

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modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_17

External Data & Images

Linkouts

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

Sap130⁰⁶⁹²⁴

semi-lethal | pupal stage, with Scer\GAL4^pnr-MD237

Sap130^GD7168

viable

Sap130^c03966

Sterility

Allele

fertile

Sap130^c03966

Phenotype manifest in

Allele

Classical Alleles ( 5 )

For All Classical Alleles Show

Allele of Sap130	Class	Mutagen	Stocks	Known lesion
Sap130⁰⁶⁹²⁴		P-element activity	1	--
Sap130^c03966		piggyBac transposase	1	--
Sap130^EY12079	loss of function allele	P-element activity	1	--
Sap130^CPTI001478		piggyBac transposase	0	Yes
Sap130^EP3348			0	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Sap130	Class	Mutagen	Stocks	Known lesion
Sap130^GD7168		in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}Atg1^EP3348

P{EPgy2}Sap130^EY12079

insertion of enhancer trap

P{PZ}Sap130⁰⁶⁹²⁴

miscellaneous insertions

PBac{681.P.FSVS-1}Sap130^CPTI001478

PBac{PB}Sap130^c03966

Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )

Terms Based on Experimental Evidence ( 2 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

mitotic spindle organization

inferred from mutant phenotype

(Goshima et al., 2007)

Cellular Component

CV term

Evidence

References

Sin3-type complex

inferred from direct assay

(Spain et al., 2010)

Terms Based on Predictions or Assertions ( 0 terms )

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Protein-protein

Interacting group

Assay

References

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Sap130 allele

Gene

References

l(3)06924⁰⁶⁹²⁴

(Carrera et al., 1998)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0262714

Orthologs

Genome-wide drosophilid orthologs

Dana\GF23573

Dere\GG15599

Dgri\anon-1

Dmoj\GI11663

Dper\GL22680

Dpse\GA10703

Dsec\GM25373

Dsim\GD14406

Dvir\GJ11341

Dwil\GK16826

Dyak\GE21925

Curated drosophilid orthologs

Dlit\BcDNA:LD21213

Dpse\GA10703

Linkouts

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

Bloomington

11705

P{PZ}Sap130⁰⁶⁹²⁴ ry⁵⁰⁶/CxD, ry^BW

20342

y¹ w^67c23; P{EPgy2}Sap130^EY12079/TM3, Sb¹ Ser¹

Harvard

PBac{PB}Sap130^c03966

VDRC

v31394

w¹¹¹⁸; P{GD7168}v31394

v31395

w¹¹¹⁸; P{GD7168}v31395

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 57 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

DPiM_LD21213

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

39455

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: Sap130 CG11006

(Cao et al., 2008)

Source for database merge of

Source for merge of: Sap130 l(3)06924

(Cao et al., 2008)

Additional comments

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly long spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

39455

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Histone deacetylase complex subunit SAP130 (IPR024137)

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0262714

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

39455

Synonyms & Secondary IDs ( 8 )

Reported As

Symbol Synonym

BcDNA:LD21213

(Rawlings, 2000.11.9, Giot et al., 2003, )

CG11006

(Goshima et al., 2007, Edwards and Mackay, 2009, Spain et al., 2010, Mourikis et al., 2010)

l(3)06924

Sap130

SAP130

(Spain et al., 2010, Cao et al., 2008)

Name Synonym

lethal (3) 06924

Sin3A-Associated Protein

(Cao et al., 2008)

Sin3A-associated protein 130

Secondary FlyBase IDs

FBgn0010907
FBgn0027534

References ( 22 )

Generate a list of

All references relating to this gene ( 22 )

List References by type

Recent research papers ( 2 )

Mourikis et al., 2010, BMC Dev. Biol. 10: 107: Modifiers of notch transcriptional activity identified by genome-wide RNAi. [FBrf0212287]
Spain et al., 2010, J. Biol. Chem. 285(35): 27457--27467: Drosophila SIN3 isoforms interact with distinct proteins and have unique biological functions. [FBrf0211588]

Show all research papers ...