FB2020_02, released Apr 14, 2020

Gene: Dmel\Arp3

Open Close
General Information
Symbol
Dmel\Arp3
Species
D. melanogaster
Name
Actin-related protein 3
Annotation Symbol
CG7558
Feature Type
protein_coding_gene
FlyBase ID
FBgn0262716
Gene Model Status
Current
Stock Availability
11 publicly available
Gene Snapshot
In progress.Contributions welcome.
Other Summaries
Also Known As

Arp66B, schwachling, Actr66B, Actin-related protein 66B

Key Links
Genomic Location
Cytogenetic map
66B6-66B6
Sequence location
3L:8,113,407..8,115,902 [-]
Recombination map

3-22

RefSeq locus
NT_037436 REGION:8113407..8115902
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the actin family. ARP3 subfamily. (P32392)
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
contributes_to actin filament binding; ATP binding; structural constituent of cytoskeleton
Summaries
Gene Group (FlyBase)
ACTIN-RELATED PROTEIN 2/3 COMPLEX -
The Actin-related protein 2/3 complex is involved in the organization of the actin cytoskeleton, in particular, the nucleation of branched actin filaments. (Adapted from FBrf0144947).
ACTIN-RELATED PROTEINS -
Actin-related proteins (Arp) share significant sequence identity with actins. Arps broadly fall into two groups: nuclear Arps, involved in chromatin remodeling; and cytoplasmic Arps, associated with actin. (Adapted from PMID:21859859).
Protein Function (UniProtKB)
Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (By similarity). Seems to contact the pointed end of the daughter actin filament (By similarity). Required during embryogenesis for the developmental migration of tail hemocytes anteriorly, along the ventral midline (PubMed:25739458).
(UniProt, P32392)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Arp3 or the JBrowse view of Dmel\Arp3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
gene
(Griemert et al., 2007)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Arp3-RA
FBtr0076737
NM_079244
1983
418
Arp3-RB
FBtr0334087
NM_001274630
1585
418
Additional Transcript Data and Comments
Reported size (kB)

1.257 (longest cDNA)

(Fyrberg et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Arp3-PA
FBpp0076460
47.0
418
5.84
NP_523968
AAF50488
Arp3-PB
FBpp0306212
47.0
418
5.84
NP_001261559
AGB94254
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

418 aa isoforms: Arp3-PA, Arp3-PB
Additional Polypeptide Data and Comments
Reported size (kDa)

418 (aa)

(Fyrberg et al., 1994)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the Arp2/3 complex.

(UniProt, P32392)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Arp3 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (19 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
contributes_to actin filament binding
inferred from biological aspect of ancestor with PANTHER:PTN000233796
(assigned by GO_Central )
(Gaudet et al., 2011)
ATP binding
inferred from electronic annotation with InterPro:IPR015623
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
structural constituent of cytoskeleton
inferred from sequence or structural similarity with HGNC:170
(Hudson and Cooley, 2002)
Biological Process (14 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
actin cytoskeleton organization
inferred from mutant phenotype
(Richardson et al., 2007)
actin filament reorganization involved in cell cycle
inferred from physical interaction with FLYBASE:Arpc1; FB:FBgn0001961
(Stevenson et al., 2002)
axonal fasciculation
inferred from mutant phenotype
(Zallen et al., 2002)
cell morphogenesis
inferred from mutant phenotype
(D'Ambrosio and Vale, 2010)
chaeta development
inferred from mutant phenotype
(Rajan et al., 2009)
intracellular protein transport
inferred from mutant phenotype
(Rajan et al., 2009)
myoblast fusion
inferred from mutant phenotype
(Berger et al., 2008, Richardson et al., 2007)
inferred from genetic interaction with FLYBASE:WASp; FB:FBgn0024273
(Berger et al., 2008)
plasma membrane tubulation
inferred from mutant phenotype
(Yan et al., 2013)
positive regulation of filopodium assembly
inferred from mutant phenotype
(Gonçalves-Pimentel et al., 2011)
positive regulation of lamellipodium assembly
inferred from mutant phenotype
(Georgiou and Baum, 2010)
positive regulation of synaptic growth at neuromuscular junction
inferred from mutant phenotype
(Koch et al., 2014)
pseudocleavage involved in syncytial blastoderm formation
inferred from physical interaction with FLYBASE:Arpc1; FB:FBgn0001961
(Stevenson et al., 2002)
terminal button organization
inferred from mutant phenotype
(Koch et al., 2014)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Arp2/3 complex-mediated actin nucleation
inferred from sequence or structural similarity with HGNC:170
(Hudson and Cooley, 2002)
inferred from biological aspect of ancestor with PANTHER:PTN000233796
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
lamellipodium
inferred from direct assay
(Iwasa and Mullins, 2007)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
Arp2/3 protein complex
inferred from biological aspect of ancestor with PANTHER:PTN000233796
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with HGNC:170
(Hudson and Cooley, 2002)
non-traceable author statement
(Vartiainen and Machesky, 2004)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
distribution deduced from reporter or direct label
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis stage S9
nurse cell ring canal
(Hudson and Cooley, 2002)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

Arp3 protein distribution was followed with an Arp3-GFP fusion protein. The GFP fusion protein is abundant in the cytoplasm of germ cells and is enriched in ring canals. Some enrichment at cortical membranes was also observed.

(Hudson and Cooley, 2002)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
lamellipodium
inferred from direct assay
(Iwasa and Mullins, 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Arp3 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 6 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Arp3
Transgenic constructs containing regulatory region of Arp3
Deletions and Duplications ( 8 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal (with Arp383F)
lethal (with Arp3515FC)
lethal (with Arp31066PC)
lethal (with Arp3EP3640)
lethal - all die before end of P-stage | recessive, with Scer\GAL4- :
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
cell migration defective, with Scer\GAL4Pxn.PS
locomotor behavior defective | third instar larval stage, with Scer\GAL4Ubi.PU
neuroanatomy defective, with Scer\GAL4C57
neuroanatomy defective | larval stage, with Scer\GAL4Ubi.PU
neuroanatomy defective | larval stage, with Scer\GAL4VGlut-OK371
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
some die during pharate adult stage | recessive, with Scer\GAL4- :
some die during pupal stage, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
anterior dorsocentral bristle | pharate adult stage, with Scer\GAL4- :
bouton, with Scer\GAL4C57
cell projection, with Scer\GAL4Pxn.PS
dorsal pharyngeal muscle
embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4Ubi.PU
embryonic/larval somatic muscle
embryonic/larval somatic muscle (with Arp3EP3640)
embryonic/larval somatic muscle (with Arp3schwachling)
embryonic hemocyte, with Scer\GAL4Pxn.PS
embryonic myoblast
external sensory organ precursor cell IIa | somatic clone
external sensory organ precursor cell IIb | somatic clone
female germline cyst | somatic clone
filopodium
fusion competent cell
interfollicle cell | somatic clone
macrochaeta | pharate adult stage, with Scer\GAL4- :
macrochaeta | somatic clone
mechanosensory chaeta | somatic clone
microchaeta | somatic clone
myoblast | embryonic stage
myoblast | embryonic stage (with Arp3EP3640)
myoblast | embryonic stage (with Arp3schwachling)
myoblast | embryonic stage (with Df(3L)ZP1)
neuromuscular junction, with Scer\GAL4C57
nurse cell & actin filament | germ-line clone, with Scer\GAL4- :
nurse cell | germline clone, with Scer\GAL4- :
nurse cell ring canal | germline clone, with Scer\GAL4- :
oocyte | germline clone, with Scer\GAL4- :
plasma membrane | embryonic stage
synapse, with Scer\GAL4C57
trichogen cell, with Scer\GAL4pnr-MD237
type Ib terminal bouton | larval stage, with Scer\GAL4Ubi.PU
type Ib terminal bouton | larval stage, with Scer\GAL4VGlut-OK371
vacuole | supernumerary, with Scer\GAL4Pxn.PS
ventral nerve cord commissure (with Df(3L)pbl-X1), with Scer\GAL4- :
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (41)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\ACTR3
14 of 15
Yes
Yes
 
Hsap\ACTR3B
12 of 15
No
Yes
 
Hsap\ACTR3C
4 of 15
No
Yes
Hsap\ACTA2
2 of 15
No
No
Hsap\ACTB
2 of 15
No
No
 
Hsap\ACTBL2
2 of 15
No
No
Hsap\ACTC1
2 of 15
No
No
 
Hsap\ACTL6A
2 of 15
No
No
 
Hsap\ACTL6B
2 of 15
No
No
 
Hsap\ACTL8
2 of 15
No
No
Hsap\ACTL9
2 of 15
No
No
Hsap\ACTL10
2 of 15
No
No
Hsap\ACTR1A
2 of 15
No
No
 
Hsap\ACTR1B
2 of 15
No
No
 
Hsap\ACTR2
2 of 15
No
No
Hsap\ACTRT3
2 of 15
No
No
Hsap\POTEE
2 of 15
No
No
Hsap\POTEF
2 of 15
No
No
Hsap\POTEI
2 of 15
No
No
Hsap\POTEJ
2 of 15
No
No
Hsap\ACTA1
1 of 15
No
No
Hsap\ACTG2
1 of 15
No
No
Hsap\ACTL7A
1 of 15
No
No
Hsap\ACTL7B
1 of 15
No
No
Hsap\ACTR6
1 of 15
No
No
Hsap\ACTR8
1 of 15
No
No
Hsap\ACTR10
1 of 15
No
No
Hsap\ACTRT1
1 of 15
No
No
Hsap\ACTRT2
1 of 15
No
No
Hsap\APOC3
1 of 15
No
Yes
Hsap\ATP10A
1 of 15
No
No
 
Hsap\BACE1
1 of 15
No
No
 
Hsap\LOC100288966
1 of 15
No
Yes
Hsap\LOC102723502
1 of 15
No
Yes
Hsap\POTEB2
1 of 15
No
No
Hsap\POTEB3
1 of 15
No
Yes
Hsap\POTEB
1 of 15
No
No
Hsap\POTEC
1 of 15
No
No
Hsap\POTED
1 of 15
No
No
Hsap\POTEG
1 of 15
No
No
Hsap\POTEH
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (28)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Actr3
13 of 15
Yes
Yes
Mmus\Actr3b
12 of 15
No
Yes
Mmus\Acta1
2 of 15
No
No
Mmus\Acta2
2 of 15
No
No
Mmus\Actbl2
2 of 15
No
No
Mmus\Actb
2 of 15
No
No
Mmus\Actc1
2 of 15
No
No
Mmus\Acte1
2 of 15
No
No
Mmus\Actg1
2 of 15
No
No
Mmus\Actg2
2 of 15
No
No
Mmus\Actl6a
2 of 15
No
No
Mmus\Actl6b
2 of 15
No
No
Mmus\Actl9
2 of 15
No
No
Mmus\Actl10
2 of 15
No
No
Mmus\Actr1b
2 of 15
No
No
Mmus\Actr2
2 of 15
No
No
Mmus\Actrt3
2 of 15
No
No
Mmus\Actl7a
1 of 15
No
No
Mmus\Actl7b
1 of 15
No
No
Mmus\Actl11
1 of 15
No
No
Mmus\Actr1a
1 of 15
No
No
Mmus\Actr6
1 of 15
No
No
Mmus\Actr8
1 of 15
No
No
Mmus\Actr10
1 of 15
No
No
Mmus\Actrt1
1 of 15
No
No
Mmus\Actrt2
1 of 15
No
No
Mmus\Ankrd36
1 of 15
No
Yes
Mmus\Atp1b1
1 of 15
No
No
Rattus norvegicus (Norway rat) (30)
Rnor\Actr3
10 of 13
Yes
Yes
Rnor\Actr3b
6 of 13
No
Yes
Rnor\Acta1
2 of 13
No
No
Rnor\Actbl2
2 of 13
No
No
Rnor\Actb
2 of 13
No
No
Rnor\Actc1
2 of 13
No
No
Rnor\Actg2
2 of 13
No
No
Rnor\Actl6a
2 of 13
No
No
Rnor\Actl6b
2 of 13
No
No
Rnor\Actl9b
2 of 13
No
No
Rnor\Actl9
2 of 13
No
No
Rnor\Actl10
2 of 13
No
No
Rnor\Actr1a
2 of 13
No
No
Rnor\Actr1b
2 of 13
No
No
Rnor\Actr2
2 of 13
No
No
Rnor\Actrt3
2 of 13
No
No
Rnor\Acta2
1 of 13
No
No
Rnor\Actg1
1 of 13
No
No
Rnor\Actl7a
1 of 13
No
No
Rnor\Actl7b
1 of 13
No
No
Rnor\Actl11
1 of 13
No
No
Rnor\Actr6
1 of 13
No
No
Rnor\Actr8
1 of 13
No
No
Rnor\Actr10
1 of 13
No
No
Rnor\Actrt1
1 of 13
No
No
Rnor\Actrt2
1 of 13
No
No
Rnor\LOC302827
1 of 13
No
Yes
Rnor\LOC100361457
1 of 13
No
No
Rnor\LOC102552318
1 of 13
No
No
Rnor\Potef
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (12)
Xtro\actr3
9 of 12
Yes
Yes
Xtro\act3
2 of 12
No
No
Xtro\acta1
2 of 12
No
No
Xtro\acta2
2 of 12
No
No
Xtro\actb
2 of 12
No
No
Xtro\actc1
2 of 12
No
No
Xtro\actg1
2 of 12
No
No
Xtro\actl6a
2 of 12
No
No
Xtro\actr1a
2 of 12
No
No
Xtro\actr2
2 of 12
No
No
Xtro\actr6
1 of 12
No
No
Xtro\actr10
1 of 12
No
No
Danio rerio (Zebrafish) (22)
Drer\actr3
9 of 15
Yes
Yes
Drer\actr3b
8 of 15
No
Yes
Drer\acta1a
2 of 15
No
No
Drer\acta1b
2 of 15
No
No
Drer\acta2
2 of 15
No
No
Drer\actb1
2 of 15
No
No
Drer\actb2
2 of 15
No
No
Drer\actc1b
2 of 15
No
No
Drer\actc1c
2 of 15
No
No
Drer\actl6a
2 of 15
No
No
Drer\actl6b
2 of 15
No
No
Drer\actr1
2 of 15
No
No
Drer\actr2a
2 of 15
No
No
Drer\actr2b
2 of 15
No
No
Drer\si:ch73-187m15.4
2 of 15
No
No
Drer\si:ch211-241j12.3
2 of 15
No
No
Drer\zgc:86709
2 of 15
No
No
Drer\zgc:101810
2 of 15
No
No
Drer\actc1a
1 of 15
No
No
Drer\actr6
1 of 15
No
No
Drer\actr8
1 of 15
No
No
Drer\actr10
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (11)
Cele\arx-1
14 of 15
Yes
Yes
Cele\act-4
2 of 15
No
No
Cele\arp-1
2 of 15
No
No
Cele\arx-2
2 of 15
No
No
Cele\act-1
1 of 15
No
No
Cele\act-2
1 of 15
No
No
Cele\act-5
1 of 15
No
No
Cele\arp-6
1 of 15
No
No
Cele\arp-11
1 of 15
No
No
Cele\F42C5.9
1 of 15
No
Yes
Cele\swsn-6
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (15)
Atha\DIS1
8 of 9
Yes
Yes
Atha\ACT2
1 of 9
No
No
Atha\ACT3
1 of 9
No
No
Atha\ACT4
1 of 9
No
No
Atha\ACT7
1 of 9
No
No
Atha\ACT8
1 of 9
No
No
Atha\ACT9
1 of 9
No
No
Atha\ACT11
1 of 9
No
No
Atha\ACT12
1 of 9
No
No
Atha\ARP2
1 of 9
No
No
Atha\ARP4
1 of 9
No
No
Atha\ARP6
1 of 9
No
No
Atha\ARP7
1 of 9
No
Yes
Atha\AT2G42100
1 of 9
No
No
Atha\AT2G42170
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (8)
Scer\ARP3
12 of 15
Yes
Yes
Scer\ACT1
1 of 15
No
No
 
Scer\ARP1
1 of 15
No
No
Scer\ARP2
1 of 15
No
No
Scer\ARP4
1 of 15
No
No
Scer\ARP7
1 of 15
No
No
Scer\ARP8
1 of 15
No
No
Scer\ARP9
1 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\arp3
11 of 12
Yes
Yes
Spom\arp9
1 of 12
No
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091908LF )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00003688
Drosophila simulans
Dsim\GD17591
Drosophila sechellia
Dsec\GM24973
Drosophila erecta
Dere\GG14922
Drosophila yakuba
Dyak\GE20375
Drosophila ananassae
Dana\GF10484
Drosophila pseudoobscura pseudoobscura
Dpse\GA20439
Drosophila persimilis
Dper\GL22484
Drosophila willistoni
Dwil\GK19880
Drosophila virilis
Dvir\GJ13271
Drosophila mojavensis
Dmoj\GI12000
Drosophila grimshawi
Dgri\GH15291
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504ZU )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17007060
Glossina morsitans
Tsetse fly
Gmor\GMOY005381
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_05597
Mayetiola destructor
Hessian fly
Mdes\Mdes000491
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL012310
Anopheles gambiae
Malaria mosquito
Agam\Actincytosk
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ015392
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ016054
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04U2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA001921
Danaus plexippus
Monarch butterfly
Dple\DPOGS207501-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL017308
Apis florea
Little honeybee
Aflo\2446443188
Apis mellifera
Western honey bee
Amel\LOC410115
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22445
Bombus terrestris
Buff-tailed bumblebee
Bter\15214079
Linepithema humile
Argentine ant
Lhum\LH21384
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001252
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG001088
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08614
Tribolium castaneum
Red flour beetle
Tcas\TC002963
Pediculus humanus
Human body louse
Phum\PHUM623380
Pediculus humanus
Human body louse
Phum\PHUM581040
Pediculus humanus
Human body louse
Phum\PHUM623180
Rhodnius prolixus
Kissing bug
Rpro\RPRC007827
Rhodnius prolixus
Kissing bug
Rpro\RPRC007826
Cimex lectularius
Bed bug
Clec\CLEC000322
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI001871
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_06167
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04QI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR002407
Stegodyphus mimosarum
African social velvet spider
Smim\KFM62346.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur11g00670
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_304740
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0BPD )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Arp3ph
Gallus gallus
Domestic chicken
Ggal\ACTR3
Gallus gallus
Domestic chicken
Ggal\ACTR3B
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (14)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Act5C
4 of 10
Act42A
4 of 10
Act57B
4 of 10
Act79B
4 of 10
Act87E
4 of 10
Act88F
4 of 10
Arp1
4 of 10
Arp2
4 of 10
Arp53D
4 of 10
Bap55
4 of 10
Arp6
3 of 10
Arp8
3 of 10
Arp10
3 of 10
Arp5
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Arp3HMS00711
    ameliorates  lung adenocarcinoma
    modeled by Hsap\KIF5B::Hsap\RETUAS.cDa
    (Das and Cagan, 2017)
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ACTR3; actin related protein 3
    14 of 15
    604222
         
        ACTR3B; actin related protein 3B
        12 of 15
             
            ACTR3C; actin related protein 3C
            4 of 15
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Interactions
                Summary of Physical Interactions
                Summary of Genetic Interactions
                esyN Network Diagram
                esyN Network Key:
                Suppression
                Enhancement
                View in Interactions Browser
                Please look at the allele data for full details of the genetic interactions
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Arp3
                enhanceable
                Cip4
                (Nahm et al., 2010)
                Arp3
                enhanceable
                WASp
                (Berger et al., 2008)
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                arm
                suppressible
                Arp3
                (Sarpal et al., 2012)
                cindr
                suppressible
                Arp3
                (Johnson et al., 2008)
                Cip4
                enhanceable
                Arp3
                (Nahm et al., 2010)
                crb
                suppressible
                Arp3
                (Flores-Benitez and Knust, 2015)
                WASp
                enhanceable
                Arp3
                (Berger et al., 2008)
                α-Cat
                suppressible
                Arp3
                (Sarpal et al., 2012)
                External Data
                Subunit Structure (UniProtKB)
                Component of the Arp2/3 complex.
                (UniProt, P32392 )
                Linkouts
                DroID - A comprehensive database of gene and protein interactions.
                MIST (genetic) - An integrated Molecular Interaction Database
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                External Data
                Linkouts
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                3L
                Recombination map

                3-22

                Cytogenetic map
                66B6-66B6
                Sequence location
                3L:8,113,407..8,115,902 [-]
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                66B6-66B6
                Limits computationally determined from genome sequence between P{PZ}l(3)0721708223&P{EP}Rac2EP3118 and P{lacW}Nmtj1C7
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                66B-66B
                (Fyrberg, 1993.5.4)
                66B-66B
                (determined by in situ hybridisation)
                (Fyrberg et al., 1994, Fyrberg and Fyrberg, 1993)
                Experimentally Determined Recombination Data
                Location

                3-22

                (FlyBase, 2016)

                3-19.0

                (Comeron et al., 2012)
                Left of (cM)
                Right of (cM)
                Notes
                Stocks and Reagents
                Stocks (11)
                Genomic Clones (17)
                 

                Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                cDNA Clones (164)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequences
                BDGP DGC clones
                Other clones
                Drosophila Genomics Resource Center cDNA clones

                For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                Other clones
                RNAi and Array Information
                Linkouts
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                Antibody Information
                Laboratory Generated Antibodies
                 
                Commercially Available Antibodies
                 
                Other Information
                Relationship to Other Genes
                Source for database identify of

                Source for identity of: Arp3 Arp66B

                (FlyBase, 2009-)
                Source for database merge of

                Source for merge of: Arp66B schwachling

                (Berger et al., 2008)
                Additional comments

                FlyBase Curator comment: 'Arp66B' renamed to 'Arp3' to rationalize the nomenclature of Actin-related protein (Arp) genes (by orthology with the mammalian and yeast genes) and better reflect community usage.

                (FlyBase, 2009-)
                Other Comments

                S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

                (Stroschein-Stevenson et al., 2006)

                dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                (Kiger et al., 2003)

                The Arp66B protein part of the Arp2/3 complex.

                (Stevenson et al., 2002)

                The Arp2/3 complex (including Arp66B) is required for actin cap expansion and pseudocleavage furrow formation during syncytial blastoderm divisions.

                (Stevenson et al., 2002)

                Isolation and characterisation of actin-related proteins Arp14D, Arp53D, Arp66B and Arp87C.

                (Fyrberg et al., 1994)

                Isolation and partial characterisation of Arp66B.

                (Fyrberg and Fyrberg, 1993)
                Origin and Etymology
                Discoverer
                Etymology
                Identification
                External Crossreferences and Linkouts ( 33 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                Other crossreferences
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                Linkouts
                DroID - A comprehensive database of gene and protein interactions.
                MIST (genetic) - An integrated Molecular Interaction Database
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                Synonyms and Secondary IDs (15)
                Reported As
                Symbol Synonym
                ARP3
                (Reed et al., 2012, Goldstein and Gunawardena, 2000)
                AcTr66B
                Actr66B
                (Fyrberg and Fyrberg, 1993)
                Arp3
                (Blake-Hedges and Megraw, 2019, Dialynas et al., 2019, Gao et al., 2019, Hurst et al., 2019, Jiang et al., 2019, Lee and Chen, 2019, Trylinski and Schweisguth, 2019, Tsarouhas et al., 2019, Caridi et al., 2018, Salazar and Yamamoto, 2018, Segal et al., 2018, Flores-Benitez and Knust, 2015, Hsiao et al., 2015, Kollmar, 2015.9.15, Levayer et al., 2015, Rodal et al., 2015, Rosa et al., 2015, Verboon et al., 2015, Haralalka et al., 2014, Koch et al., 2014, Ingerman et al., 2013, Leibfried et al., 2013, Yan et al., 2013, Abmayr and Pavlath, 2012, Giagtzoglou et al., 2012, Reed et al., 2012, Sarpal et al., 2012, Zoller and Schulz, 2012, Isaji et al., 2011, Rotkopf et al., 2011, Eriksson et al., 2010, Serio et al., 2010, Massarwa et al., 2009, Rajan et al., 2009, Berger et al., 2008, Richardson and Baylies, 2008, Schäfer et al., 2008, Iwasa and Mullins, 2007, Jovceva et al., 2007, Rajan et al., 2007, Richardson et al., 2007, Kollmar and Odronitz, 2006, Price et al., 2006, Frank et al., 2005, Noguchi et al., 2005, Beltzner and Pollard, 2004, Biyasheva et al., 2004, Bogdan et al., 2004, Noguchi et al., 2004, Vartiainen and Machesky, 2004, Grevengoed et al., 2003, Lundquist, 2003, Rogers et al., 2003, Hudson and Cooley, 2002, Hudson and Cooley, 2002, Jacinto et al., 2002, Kiehart and Franke, 2002, Miller, 2002, Rogat and Miller, 2002, Rorth, 2002, Stevenson et al., 2002, Tal et al., 2002, Zallen et al., 2002, Hudson and Cooley, 2001)
                Arp66B
                (Das and Cagan, 2017, Gonzalez et al., 2014, Lee et al., 2014, Antunes et al., 2013, Kwon et al., 2013, Abmayr and Pavlath, 2012, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Gonçalves-Pimentel et al., 2011, Rohn et al., 2011, Rotkopf et al., 2011, D'Ambrosio and Vale, 2010, Berger et al., 2008, Bakal et al., 2007, Stuart et al., 2007, Stroschein-Stevenson et al., 2006)
                CG7558
                (Kolkhof et al., 2017, Hosono et al., 2015, Abmayr and Pavlath, 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Goldstein and Gunawardena, 2000)
                anon-WO0172774.138
                arp3
                (Rudolf et al., 2014, Herszterg et al., 2013, Hatan et al., 2011, Hornbruch-Freitag et al., 2011, Nahm et al., 2010, Leibfried et al., 2008, Noguchi et al., 2008, Frank et al., 2006, Rogat and Miller, 2002, Frankel and Mooseker, 1996)
                arp66B
                (Sánchez-Soriano et al., 2010, Georgiou et al., 2008, Johnson et al., 2008, Wang et al., 2008, Fyrberg, 1993.5.4)
                arp66b
                (Georgiou and Baum, 2010)
                schwachling
                Name Synonyms
                Actin-related protein
                (Fyrberg and Fyrberg, 1993)
                Actin-related protein 3
                Actin-related protein 66B
                (Fyrberg et al., 1994)
                actin-related protein-3
                (Rajan et al., 2009)
                schwachling
                (Berger et al., 2008, Griemert et al., 2007)
                Secondary FlyBase IDs
                • FBgn0086714
                • FBgn0011744
                • FBgn0046458
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                References (156)