FlyBase Gene Report: Dmel\Arp3

General Information

Symbol

Dmel\Arp3

Species

D. melanogaster

Name

Actin-related protein 3

Annotation Symbol

CG7558

Feature Type

protein_coding_gene

FlyBase ID

FBgn0262716

Gene Model Status

Stock Availability

11 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Arp66B, schwachling, Actin-related protein 66B, Actr66B

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:8,113,407..8,115,902 [-]

Recombination map

3-22

RefSeq locus

NT_037436 REGION:8113407..8115902

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

contributes_to actin filament binding

inferred from biological aspect of ancestor with PANTHER:PTN000233796

(assigned by GO_Central )

(Gaudet et al., 2011)

enables ATP binding

inferred from electronic annotation with InterPro:IPR015623

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables structural constituent of cytoskeleton

inferred from sequence or structural similarity with HGNC:170

(Hudson and Cooley, 2002)

Biological Process (14 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

involved_in actin cytoskeleton organization

inferred from mutant phenotype

(Richardson et al., 2007)

involved_in actin filament reorganization involved in cell cycle

inferred from physical interaction with FLYBASE:Arpc1; FB:FBgn0001961

(Stevenson et al., 2002)

involved_in axonal fasciculation

inferred from mutant phenotype

(Zallen et al., 2002)

involved_in cell morphogenesis

inferred from mutant phenotype

(D'Ambrosio and Vale, 2010)

involved_in chaeta development

inferred from mutant phenotype

(Rajan et al., 2009)

involved_in intracellular protein transport

inferred from mutant phenotype

(Rajan et al., 2009)

involved_in myoblast fusion

inferred from mutant phenotype

(Berger et al., 2008, Richardson et al., 2007)

inferred from genetic interaction with FLYBASE:WASp; FB:FBgn0024273

(Berger et al., 2008)

involved_in plasma membrane tubulation

inferred from mutant phenotype

(Yan et al., 2013)

involved_in positive regulation of filopodium assembly

inferred from mutant phenotype

(Gonçalves-Pimentel et al., 2011)

involved_in positive regulation of lamellipodium assembly

inferred from mutant phenotype

(Georgiou and Baum, 2010)

involved_in positive regulation of synaptic growth at neuromuscular junction

inferred from mutant phenotype

(Koch et al., 2014)

involved_in pseudocleavage involved in syncytial blastoderm formation

inferred from physical interaction with FLYBASE:Arpc1; FB:FBgn0001961

(Stevenson et al., 2002)

involved_in terminal button organization

inferred from mutant phenotype

(Koch et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in Arp2/3 complex-mediated actin nucleation

inferred from sequence or structural similarity with HGNC:170

(Hudson and Cooley, 2002)

inferred from biological aspect of ancestor with PANTHER:PTN000233796

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in lamellipodium

inferred from direct assay

(Iwasa and Mullins, 2007)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of Arp2/3 protein complex

inferred from biological aspect of ancestor with PANTHER:PTN000233796

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with HGNC:170

(Hudson and Cooley, 2002)

non-traceable author statement

(Vartiainen and Machesky, 2004)

Gene Group (FlyBase)

ACTIN-RELATED PROTEIN 2/3 COMPLEX

ACTIN-RELATED PROTEINS

Protein Family (UniProt)

Belongs to the actin family. ARP3 subfamily. (P32392)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

ACTIN-RELATED PROTEIN 2/3 COMPLEX -: The Actin-related protein 2/3 complex is involved in the organization of the actin cytoskeleton, in particular, the nucleation of branched actin filaments. (Adapted from FBrf0144947).
ACTIN-RELATED PROTEINS -: Actin-related proteins (Arp) share significant sequence identity with actins. Arps broadly fall into two groups: nuclear Arps, involved in chromatin remodeling; and cytoplasmic Arps, associated with actin. (Adapted from PMID:21859859).

Protein Function (UniProtKB)

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks (By similarity). Seems to contact the pointed end of the daughter actin filament (By similarity). Required during embryogenesis for the developmental migration of tail hemocytes anteriorly, along the ventral midline (PubMed:25739458).

(UniProt, P32392)

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\Arp3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

(Griemert et al., 2007)

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0076737

1983

418

FBtr0334087

1585

418

Additional Transcript Data and Comments

Reported size (kB)

1.257 (longest cDNA)

(Fyrberg et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0076460

47.0

418

5.84

FBpp0306212

47.0

418

5.84

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

418 aa isoforms: Arp3-PA, Arp3-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

418 (aa)

(Fyrberg et al., 1994)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Arp2/3 complex.

(UniProt, P32392)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

P32392

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Arp3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Arp3_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

distribution deduced from reporter or direct label

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S9

nurse cell ring canal

• cytoplasm

(Hudson and Cooley, 2002)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Arp3 protein distribution was followed with an Arp3-GFP fusion protein. The GFP fusion protein is abundant in the cytoplasm of germ cells and is enriched in ring canals. Some enrichment at cortical membranes was also observed.

(Hudson and Cooley, 2002)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in lamellipodium

inferred from direct assay

(Iwasa and Mullins, 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Arp3 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Arp3

View exonic expression by tissue for Dmel\Arp3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7558

Images

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Arp3

Transgenic constructs containing regulatory region of Arp3

Deletions and Duplications ( 8 )

Disrupted in

(Rajan et al., 2009)

(Ryder, 2004.4.26)

(Rajan et al., 2009)

(Rajan et al., 2009)

(Rajan et al., 2009, Berger et al., 2008, Fyrberg et al., 1994)

(Rajan et al., 2009, Berger et al., 2008)

Not disrupted in

(Rajan et al., 2009)

(Rajan et al., 2009)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Arp3^83F)

Arp3^EP3640

lethal (with Arp3^515FC)

Arp3^EP3640

lethal (with Arp3^1066PC)

Arp3^EP3640

lethal (with Arp3^EP3640)

lethal - all die before end of P-stage | recessive, with Scer\GAL4^- :

Arp3^EP3640

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Arp3^GD12273

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Arp3^GD12273

viable, with Scer\GAL4^tin.CΔ4

Arp3^GD12273

Other Phenotypes

Allele

cell migration defective, with Scer\GAL4^Pxn.PS

Arp3^GD12273

locomotor behavior defective | third instar larval stage, with Scer\GAL4^Ubi.PU

Arp3^GD12273

neuroanatomy defective, with Scer\GAL4^C57

Arp3^GD12273

neuroanatomy defective | larval stage, with Scer\GAL4^Ubi.PU

Arp3^GD12273

neuroanatomy defective | larval stage, with Scer\GAL4^VGlut-OK371

Arp3^GD12273

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

Arp3^GD12273

some die during pharate adult stage | recessive, with Scer\GAL4^- :

Arp3^EP3640

some die during pupal stage, with Scer\GAL4^pnr-MD237

Arp3^GD12273

visible, with Scer\GAL4^pnr-MD237

Arp3^GD12273

Phenotype manifest in

Allele

anterior dorsocentral bristle | pharate adult stage, with Scer\GAL4^- :

Arp3^EP3640

bouton, with Scer\GAL4^C57

Arp3^GD12273

cell projection, with Scer\GAL4^Pxn.PS

Arp3^GD12273

dorsal pharyngeal muscle

Arp3^schwachling

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^Ubi.PU

Arp3^GD12273

embryonic/larval somatic muscle

Arp3^schwachling

embryonic/larval somatic muscle (with Arp3^EP3640)

Arp3^schwachling

embryonic/larval somatic muscle (with Arp3^schwachling)

Arp3^EP3640

embryonic hemocyte, with Scer\GAL4^Pxn.PS

Arp3^GD12273

embryonic myoblast

Arp3^EP3640

external sensory organ precursor cell IIa | somatic clone

external sensory organ precursor cell IIb | somatic clone

female germline cyst | somatic clone

Arp3^515FC

fusion competent cell

Arp3^schwachling

macrochaeta | pharate adult stage, with Scer\GAL4^- :

Arp3^EP3640

macrochaeta | somatic clone

Arp3^83F

mechanosensory chaeta | somatic clone

microchaeta | somatic clone

Arp3^83F

myoblast | embryonic stage

Arp3^schwachling

myoblast | embryonic stage (with Arp3^EP3640)

Arp3^schwachling

myoblast | embryonic stage (with Arp3^schwachling)

Arp3^EP3640

myoblast | embryonic stage (with Df(3L)ZP1)

Arp3^schwachling

neuromuscular junction, with Scer\GAL4^C57

Arp3^GD12273

nurse cell & actin filament | germ-line clone, with Scer\GAL4^- :

Arp3^EP3640

nurse cell | germline clone, with Scer\GAL4^- :

Arp3^EP3640

nurse cell ring canal | germline clone, with Scer\GAL4^- :

Arp3^EP3640

oocyte | germline clone, with Scer\GAL4^- :

Arp3^EP3640

plasma membrane | embryonic stage

Arp3^schwachling

stalk follicle cell | somatic clone

Arp3^515FC

synapse, with Scer\GAL4^C57

Arp3^GD12273

trichogen cell, with Scer\GAL4^pnr-MD237

Arp3^GD12273

type Ib terminal bouton | larval stage, with Scer\GAL4^Ubi.PU

Arp3^GD12273

type Ib terminal bouton | larval stage, with Scer\GAL4^VGlut-OK371

Arp3^GD12273

vacuole | supernumerary, with Scer\GAL4^Pxn.PS

Arp3^GD12273

ventral nerve cord commissure (with Df(3L)pbl-X1), with Scer\GAL4^- :

Arp3^EP3640

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (41)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

14 of 15

Yes

Yes

1

12 of 15

No

Yes

1

4 of 15

No

Yes

2 of 15

No

No

2 of 15

No

No

4

2 of 15

No

No

2 of 15

No

No

1

2 of 15

No

No

2

2 of 15

No

No

1

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

1

2 of 15

No

No

1

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1

1 of 15

No

No

6

Hsap\LOC100288966

1 of 15

No

Yes

Hsap\LOC102723502

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (28)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

12 of 15

No

Yes

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

Rattus norvegicus (Norway rat) (30)

10 of 13

Yes

Yes

6 of 13

No

Yes

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

Rnor\LOC100361457

1 of 13

No

No

Rnor\LOC102552318

1 of 13

No

No

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (12)

9 of 12

Yes

Yes

2 of 12

No

No

2 of 12

No

No

2 of 12

No

No

2 of 12

No

No

2 of 12

No

No

2 of 12

No

No

2 of 12

No

No

2 of 12

No

No

2 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Danio rerio (Zebrafish) (22)

9 of 15

Yes

Yes

8 of 15

No

Yes

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

Drer\si:ch73-187m15.4

2 of 15

No

No

Drer\si:ch211-241j12.3

2 of 15

No

No

2 of 15

No

No

Drer\zgc:101810

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (11)

14 of 15

Yes

Yes

2 of 15

No

No

2 of 15

No

No

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (15)

8 of 9

Yes

Yes

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

No

1 of 9

No

Yes

1 of 9

No

No

1 of 9

No

No

Saccharomyces cerevisiae (Brewer's yeast) (8)

12 of 15

Yes

Yes

1 of 15

No

No

0

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

11 of 12

Yes

Yes

1 of 12

No

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091908LF )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00003688

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504ZU )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY005381

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_05597

Mayetiola destructor

Hessian fly

Mdes\Mdes000491

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL012310

Anopheles gambiae

Malaria mosquito

Agam\Actincytosk

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ015392

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ016054

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04U2 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001921

Danaus plexippus

Monarch butterfly

Dple\DPOGS207501-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL017308

Apis florea

Little honeybee

Aflo\2446443188

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001252

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001088

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM623380

Pediculus humanus

Human body louse

Phum\PHUM581040

Pediculus humanus

Human body louse

Phum\PHUM623180

Rhodnius prolixus

Kissing bug

Rpro\RPRC007827

Rhodnius prolixus

Kissing bug

Rpro\RPRC007826

Cimex lectularius

Bed bug

Clec\CLEC000322

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001871

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_06167

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04QI )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR002407

Stegodyphus mimosarum

African social velvet spider

Smim\KFM62346.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur11g00670

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_304740

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0BPD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (14)

Gene Symbol

Score

Source

Alignment

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

3 of 10

3 of 10

3 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

ameliorates lung adenocarcinoma

modeled by Hsap\KIF5B::Hsap\RET^UAS.cDa

(Das and Cagan, 2017)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ACTR3; actin related protein 3

14 of 15

ACTR3B; actin related protein 3B

12 of 15

ACTR3C; actin related protein 3C

4 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, anti tag western blot

(Caridi et al., 2018)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, anti tag western blot

(Caridi et al., 2018)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Nahm et al., 2010)

enhanceable

(Berger et al., 2008)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Sarpal et al., 2012)

suppressible

(Johnson et al., 2008)

enhanceable

(Nahm et al., 2010)

suppressible

(Flores-Benitez and Knust, 2015)

enhanceable

(Berger et al., 2008)

suppressible

(Sarpal et al., 2012)

External Data

Subunit Structure (UniProtKB)

Component of the Arp2/3 complex.

(UniProt, P32392 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262716

MIST (genetic) - An integrated Molecular Interaction Database

Arp3

MIST (protein-protein) - An integrated Molecular Interaction Database

Arp3

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3L

Recombination map

3-22

Cytogenetic map

Sequence location

3L:8,113,407..8,115,902 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66B6-66B6

Limits computationally determined from genome sequence between P{PZ}l(3)07217⁰⁸²²³&P{EP}Rac2^EP3118 and P{lacW}Nmt^j1C7

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

66B-66B

(Fyrberg, 1993.5.4)

66B-66B

(determined by in situ hybridisation)

(Fyrberg et al., 1994, Fyrberg and Fyrberg, 1993)

Experimentally Determined Recombination Data

Location

3-22

(FlyBase, 2016)

3-19.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

39721

P{UASp-Arp3.GFP}1, w^*

39723

w^*; P{UASp-Arp3.GFP}2

53972

y¹ v¹; P{TRiP.HMJ21357}attP40

17149

w¹¹¹⁸; P{EP}Arp3^EP3640/TM6B, Tb¹

39722

w^*; P{UASp-Arp3.GFP}3/TM6C, Sb¹ Tb¹

32921

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00711}attP2

39726

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}80B Arp3^83F/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹

39727

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}80B Arp3^515FC/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹

v108951

P{KK102278}VIE-260B

v35258

w¹¹¹⁸; P{GD12273}v35258

v35260

w¹¹¹⁸; P{GD12273}v35260

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (164)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD35711

Other clones

DPiM_LD35711

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0262716

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38898

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Arp3 Arp66B

(FlyBase, 2009-)

Source for database merge of

Source for merge of: Arp66B schwachling

(Berger et al., 2008)

Additional comments

FlyBase Curator comment: 'Arp66B' renamed to 'Arp3' to rationalize the nomenclature of Actin-related protein (Arp) genes (by orthology with the mammalian and yeast genes) and better reflect community usage.

(FlyBase, 2009-)

Other Comments

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

The Arp66B protein part of the Arp2/3 complex.

(Stevenson et al., 2002)

The Arp2/3 complex (including Arp66B) is required for actin cap expansion and pseudocleavage furrow formation during syncytial blastoderm divisions.

(Stevenson et al., 2002)

Isolation and characterisation of actin-related proteins Arp14D, Arp53D, Arp66B and Arp87C.

(Fyrberg et al., 1994)

Isolation and partial characterisation of Arp66B.

(Fyrberg and Fyrberg, 1993)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 33 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38898

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P32392

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0262716

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Arp3_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7558

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38898

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002963

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG7558

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P32392

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262716

MIST (genetic) - An integrated Molecular Interaction Database

Arp3

MIST (protein-protein) - An integrated Molecular Interaction Database

Arp3

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

ARP3

(Reed et al., 2012, Goldstein and Gunawardena, 2000)

AcTr66B

Actr66B

(Fyrberg and Fyrberg, 1993)

Arp3

(Chougule et al., 2020, Davidson and Wood, 2020, Deng et al., 2020, Lv et al., 2020, Meltzer and Schuldiner, 2020.6.25, Schroeder et al., 2020, Thestrup et al., 2020, Verboon et al., 2020, Wang et al., 2020, Blake-Hedges and Megraw, 2019, Dialynas et al., 2019, Gao et al., 2019, Hurst et al., 2019, Jiang et al., 2019, Lee and Chen, 2019, Trylinski and Schweisguth, 2019, Tsarouhas et al., 2019, Caridi et al., 2018, Salazar and Yamamoto, 2018, Segal et al., 2018, Flores-Benitez and Knust, 2015, Hsiao et al., 2015, Kollmar, 2015.9.15, Levayer et al., 2015, Rodal et al., 2015, Rosa et al., 2015, Verboon et al., 2015, Haralalka et al., 2014, Koch et al., 2014, Ingerman et al., 2013, Leibfried et al., 2013, Yan et al., 2013, Abmayr and Pavlath, 2012, Giagtzoglou et al., 2012, Reed et al., 2012, Sarpal et al., 2012, Zoller and Schulz, 2012, Isaji et al., 2011, Rotkopf et al., 2011, Eriksson et al., 2010, Serio et al., 2010, Massarwa et al., 2009, Rajan et al., 2009, Berger et al., 2008, Richardson and Baylies, 2008, Schäfer et al., 2008, Iwasa and Mullins, 2007, Jovceva et al., 2007, Rajan et al., 2007, Richardson et al., 2007, Kollmar and Odronitz, 2006, Price et al., 2006, Frank et al., 2005, Noguchi et al., 2005, Beltzner and Pollard, 2004, Biyasheva et al., 2004, Bogdan et al., 2004, Noguchi et al., 2004, Vartiainen and Machesky, 2004, Grevengoed et al., 2003, Lundquist, 2003, Rogers et al., 2003, Hudson and Cooley, 2002, Hudson and Cooley, 2002, Jacinto et al., 2002, Kiehart and Franke, 2002, Miller, 2002, Rogat and Miller, 2002, Rorth, 2002, Stevenson et al., 2002, Tal et al., 2002, Zallen et al., 2002, Hudson and Cooley, 2001)

Arp66B

(Das and Cagan, 2017, Gonzalez et al., 2014, Lee et al., 2014, Antunes et al., 2013, Kwon et al., 2013, Abmayr and Pavlath, 2012, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Gonçalves-Pimentel et al., 2011, Rohn et al., 2011, Rotkopf et al., 2011, D'Ambrosio and Vale, 2010, Berger et al., 2008, Bakal et al., 2007, Stuart et al., 2007, Stroschein-Stevenson et al., 2006)

CG7558

(Kolkhof et al., 2017, Hosono et al., 2015, Abmayr and Pavlath, 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Goldstein and Gunawardena, 2000)

anon-WO0172774.138

arp3

(Rudolf et al., 2014, Herszterg et al., 2013, Hatan et al., 2011, Hornbruch-Freitag et al., 2011, Nahm et al., 2010, Leibfried et al., 2008, Noguchi et al., 2008, Frank et al., 2006, Rogat and Miller, 2002, Frankel and Mooseker, 1996)

arp66B

(Sánchez-Soriano et al., 2010, Georgiou et al., 2008, Johnson et al., 2008, Wang et al., 2008, Fyrberg, 1993.5.4)

arp66b

(Georgiou and Baum, 2010)

schwachling

Name Synonyms

Actin-related protein

(Fyrberg and Fyrberg, 1993)

Actin-related protein 3

Actin-related protein 66B

(Fyrberg et al., 1994)

actin-related protein-3

(Rajan et al., 2009)

schwachling

(Berger et al., 2008, Griemert et al., 2007)

Secondary FlyBase IDs

FBgn0086714
FBgn0011744
FBgn0046458

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (168)