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Gene Dmel\mbf1

General Information
Symbol	Dmel\mbf1	Species	D. melanogaster
Name	multiprotein bridging factor 1	Annotation symbol	CG4143
Feature type	protein_coding_gene	FlyBase ID	FBgn0262732
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	MRE4
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	73A9-73A9	Sequence location	3L:16,567,536..16,569,971 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene multiprotein bridging factor 1 is referred to in FlyBase by the symbol Dmel\mbf1 (CG4143, FBgn0262732). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding, bridging; transcription coactivator activity. There is experimental evidence that it is involved in the biological process: central nervous system development; positive regulation of transcription from RNA polymerase II promoter; dendrite morphogenesis; open tracheal system development. 12 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; presumptive embryonic/larval tracheal system; mesothoracic tergum; tormogen cell; presumptive embryonic/larval central nervous system. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Helix-turn-helix type 3; Lambda repressor-like, DNA-binding domain; Multiprotein bridging factor 1, N-terminal. Gene sequence location is 3L:16567536..16569971.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 73A9-73A9   Limits computationally determined from genome sequence between P{lacW}l(3)j10E8j10E8&P{PZ}l(3)1053210532 and P{PZ}Baldspot02281
Experimentally Determined Cytological Location
Cytogenetic map Notes References 73A-73A     (Jindra, 1999.8.18) 73A-73A     (Hiromi, 1999.3.11)
Experimentally Determined Recombination Data
Location
Left of (cM)	tra (Jindra, 1999.3.12)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mbf1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Gene model reviewed during 5.45 Stop-codon suppression (UGA) postulated; FBrf0216884. gene_with_stop_codon_read_through ; SO:0000697 Low-frequency RNA-seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mbf1-RA FBtr0075393  NM_079386   1085   145 mbf1-RB FBtr0075394  NM_168673   1648   145 mbf1-RC FBtr0305569  NM_001259867   1225   145 mbf1-RD FBtr0305570  NM_001259866   1548   145 mbf1-RE FBtr0330137  NM_001275023   1085   188
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mbf1-PA   FBpp0075151   16.1   145   10.77     NP_524110   AAF49449 mbf1-PB   FBpp0075152   16.1   145   10.77     NP_730178   AAN11755 mbf1-PC   FBpp0294020   16.1   145   10.77     NP_001246796   AFH04467 mbf1-PD   FBpp0294021   16.1   145   10.77     NP_001246795   AFH04466 mbf1-PE   FBpp0303170   20.4   188   10.96     NP_001261952   AGB94645
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Helix-turn-helix (IPR001387) Lambda repressor-like, DNA-binding domain (IPR010982) Multiprotein bridging factor 1, N-terminal (IPR013729)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA694681   AA820267   AE014296 AAF49449   AE014296 AAN11755   AB031273 BAA83523   AF132156 AAD34744   AE014296 AFH04466   AE014296 AFH04467   AE014296 AGB94645   AI106720   AI113713   AI455375   AQ073116   AW940346   AW941127   AY118461 AAM49830   AY232177   BE975089   BF486861   BF501704   BG641212   BI163750   BI213801   BI240904   BI366597   BI570361   BI589812   BI590328   BI590363   BI590619   BI590631   BI606810   BI614965   BI616847   BI624024   BI633554   BI637316   BI639641   BI641744   BP557299   BQ103058   BQ103085   BX047612   CK570280   CO181203   CO262248   CO262253   CO264502   CO270497   CO275684   CO281292   CO283264   CO287117   CO290295   CO291275   CO312833   CO316934   CO332447   CO333014   CO333749   CO341523   CZ488585   CZ488586   CZ490351   EC059447   EC066167   EC067887   EC070515   EC073470   EC081434   EC086660   EC195176   EC196430   EC202526   EC205429   EC209556   EC219355   EC225475   EC229198   EC230634   EC238539   EC242425   EC242861   EC244299   EC245344   EC252199   ES688769   EV593080   GH780966   GH869208
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9XZ03 Q8MT00
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference larval stage   central nervous system   (Jindra et al., 2004) imaginal disc   (Jindra et al., 2004) testis   (Jindra et al., 2004) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Jindra et al., 2004)
Additional Descriptive Data
	Expression of mbf1 protein is high in the central nervous system, imaginal discs, and gonads, but absent in fat bodies. (Jindra et al., 2004)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Jindra et al., 2004) nucleus inferred from direct assay (Jindra et al., 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    132 central nervous system, larvae L3    86 central nervous system, pupae P8    109 head, virgin 1-day female    36 head, virgin 4-day female    32 head, virgin 20-day female    21 head, mated 1-day female    30 head, mated 4-day female    31 head, mated 20-day female    23 head, mated 1-day male    39 head, mated 4-day male    37 head, mated 20-day male    40 salivary gland, larvae L3 wandering    26 salivary gland, white prepupae    35 digestive system, larvae L3 wandering    38 digestive system, 1-day adult    43 digestive system, 4-day adult    52 digestive system, 20-day adult    39 fat body, larvae L3 wandering    24 fat body, white prepupae    46 fat body, pupae P8    78 carcass, larvae L3 wandering    52 carcass, 1-day adult    25 carcass, 4-day adult    36 carcass, 20-day adult    31 ovary, virgin 4-day female    116 ovary, mated 4-day female    112 testis, mated 4-day male    182 accessory gland, mated 4-day male    49 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (132) central nervous system, larvae L3  (86) central nervous system, pupae P8  (109) head, virgin 1-day female  (36) head, virgin 4-day female  (32) head, virgin 20-day female    21 head, mated 1-day female  (30) head, mated 4-day female  (31) head, mated 20-day female    23 head, mated 1-day male  (39) head, mated 4-day male  (37) head, mated 20-day male  (40) salivary gland, larvae L3 wandering  26 salivary gland, white prepupae  (35) digestive system, larvae L3 wandering  (38) digestive system, 1-day adult  (43) digestive system, 4-day adult  (52) digestive system, 20-day adult  (39) fat body, larvae L3 wandering    24 fat body, white prepupae  (46) fat body, pupae P8  (78) carcass, larvae L3 wandering  (52) carcass, 1-day adult    25 carcass, 4-day adult  (36) carcass, 20-day adult  (31) ovary, virgin 4-day female  (116) ovary, mated 4-day female  (112) testis, mated 4-day male  (182) accessory gland, mated 4-day male  (49) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (132) central nervous system, larvae L3    86 central nervous system, pupae P8  (109) head, virgin 1-day female    36 head, virgin 4-day female    32 head, virgin 20-day female    21 head, mated 1-day female    30 head, mated 4-day female    31 head, mated 20-day female    23 head, mated 1-day male    39 head, mated 4-day male    37 head, mated 20-day male    40 salivary gland, larvae L3 wandering    26 salivary gland, white prepupae    35 digestive system, larvae L3 wandering    38 digestive system, 1-day adult    43 digestive system, 4-day adult    52 digestive system, 20-day adult    39 fat body, larvae L3 wandering    24 fat body, white prepupae    46 fat body, pupae P8    78 carcass, larvae L3 wandering    52 carcass, 1-day adult    25 carcass, 4-day adult    36 carcass, 20-day adult    31 ovary, virgin 4-day female  (116) ovary, mated 4-day female  (112) testis, mated 4-day male  (182) accessory gland, mated 4-day male    49 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    132 central nervous system, larvae L3    86 central nervous system, pupae P8    109 head, virgin 1-day female    36 head, virgin 4-day female    32 head, virgin 20-day female    21 head, mated 1-day female    30 head, mated 4-day female    31 head, mated 20-day female    23 head, mated 1-day male    39 head, mated 4-day male    37 head, mated 20-day male    40 salivary gland, larvae L3 wandering    26 salivary gland, white prepupae    35 digestive system, larvae L3 wandering    38 digestive system, 1-day adult    43 digestive system, 4-day adult    52 digestive system, 20-day adult    39 fat body, larvae L3 wandering    24 fat body, white prepupae    46 fat body, pupae P8    78 carcass, larvae L3 wandering    52 carcass, 1-day adult    25 carcass, 4-day adult    36 carcass, 20-day adult    31 ovary, virgin 4-day female    116 ovary, mated 4-day female    112 testis, mated 4-day male    182 accessory gland, mated 4-day male    49 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    132 central nervous system, larvae L3    86 central nervous system, pupae P8    109 head, virgin 1-day female    36 head, virgin 4-day female    32 head, virgin 20-day female    21 head, mated 1-day female    30 head, mated 4-day female    31 head, mated 20-day female    23 head, mated 1-day male    39 head, mated 4-day male    37 head, mated 20-day male    40 salivary gland, larvae L3 wandering    26 salivary gland, white prepupae    35 digestive system, larvae L3 wandering    38 digestive system, 1-day adult    43 digestive system, 4-day adult    52 digestive system, 20-day adult    39 fat body, larvae L3 wandering    24 fat body, white prepupae    46 fat body, pupae P8    78 carcass, larvae L3 wandering    52 carcass, 1-day adult    25 carcass, 4-day adult    36 carcass, 20-day adult    31 ovary, virgin 4-day female    116 ovary, mated 4-day female    112 testis, mated 4-day male    182 accessory gland, mated 4-day male    49 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (132) central nervous system, larvae L3  (86) central nervous system, pupae P8  (109) head, virgin 1-day female  (36) head, virgin 4-day female  32 head, virgin 20-day female    21 head, mated 1-day female  30 head, mated 4-day female  31 head, mated 20-day female    23 head, mated 1-day male  (39) head, mated 4-day male  (37) head, mated 20-day male  (40) salivary gland, larvae L3 wandering  26 salivary gland, white prepupae  (35) digestive system, larvae L3 wandering  (38) digestive system, 1-day adult  (43) digestive system, 4-day adult  (52) digestive system, 20-day adult  (39) fat body, larvae L3 wandering    24 fat body, white prepupae  (46) fat body, pupae P8  (78) carcass, larvae L3 wandering  (52) carcass, 1-day adult    25 carcass, 4-day adult  (36) carcass, 20-day adult  31 ovary, virgin 4-day female  (116) ovary, mated 4-day female  (112) testis, mated 4-day male  (182) accessory gland, mated 4-day male  (49) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  132 central nervous system, larvae L3    86 central nervous system, pupae P8  109 head, virgin 1-day female    36 head, virgin 4-day female    32 head, virgin 20-day female    21 head, mated 1-day female    30 head, mated 4-day female    31 head, mated 20-day female    23 head, mated 1-day male    39 head, mated 4-day male    37 head, mated 20-day male    40 salivary gland, larvae L3 wandering    26 salivary gland, white prepupae    35 digestive system, larvae L3 wandering    38 digestive system, 1-day adult    43 digestive system, 4-day adult    52 digestive system, 20-day adult    39 fat body, larvae L3 wandering    24 fat body, white prepupae    46 fat body, pupae P8    78 carcass, larvae L3 wandering    52 carcass, 1-day adult    25 carcass, 4-day adult    36 carcass, 20-day adult    31 ovary, virgin 4-day female  116 ovary, mated 4-day female  112 testis, mated 4-day male  (182) accessory gland, mated 4-day male    49 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    132 central nervous system, larvae L3    86 central nervous system, pupae P8    109 head, virgin 1-day female    36 head, virgin 4-day female    32 head, virgin 20-day female    21 head, mated 1-day female    30 head, mated 4-day female    31 head, mated 20-day female    23 head, mated 1-day male    39 head, mated 4-day male    37 head, mated 20-day male    40 salivary gland, larvae L3 wandering    26 salivary gland, white prepupae    35 digestive system, larvae L3 wandering    38 digestive system, 1-day adult    43 digestive system, 4-day adult    52 digestive system, 20-day adult    39 fat body, larvae L3 wandering    24 fat body, white prepupae    46 fat body, pupae P8    78 carcass, larvae L3 wandering    52 carcass, 1-day adult    25 carcass, 4-day adult    36 carcass, 20-day adult    31 ovary, virgin 4-day female    116 ovary, mated 4-day female    112 testis, mated 4-day male    182 accessory gland, mated 4-day male    49 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    16 embryo 04-06hr    35 embryo 06-08hr    37 embryo 08-10hr    40 embryo 10-12hr    50 embryo 12-14hr    46 embryo 14-16hr    38 embryo 16-18hr    42 embryo 18-20hr    34 embryo 20-22hr    34 embryo 22-24hr    37 larva L1    46 larva L2    52 larva L3 12hr old    44 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    48 white prepupae new    45 white prepupae 12hr    55 white prepupae 24hr    70 pupae 2d postWPP    71 pupae 3d postWPP    55 pupae 4d postWPP    28 adult male 01day    40 adult male 05day    51 adult male 30day    45 adult female 01day    39 adult female 05day    71 adult female 30day    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (67) embryo 02-04hr    16 embryo 04-06hr  (35) embryo 06-08hr  (37) embryo 08-10hr  (40) embryo 10-12hr  (50) embryo 12-14hr  (46) embryo 14-16hr  (38) embryo 16-18hr  (42) embryo 18-20hr  (34) embryo 20-22hr  (34) embryo 22-24hr  (37) larva L1  (46) larva L2  (52) larva L3 12hr old  (44) larva L3 puffstage 1-2  (31) larva L3 puffstage 3-6  (39) larva L3 puffstage 7-9  (48) white prepupae new  (45) white prepupae 12hr  (55) white prepupae 24hr  (70) pupae 2d postWPP  (71) pupae 3d postWPP  (55) pupae 4d postWPP  (28) adult male 01day  (40) adult male 05day  (51) adult male 30day  (45) adult female 01day  (39) adult female 05day  (71) adult female 30day  (68) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    16 embryo 04-06hr    35 embryo 06-08hr    37 embryo 08-10hr    40 embryo 10-12hr    50 embryo 12-14hr    46 embryo 14-16hr    38 embryo 16-18hr    42 embryo 18-20hr    34 embryo 20-22hr    34 embryo 22-24hr    37 larva L1    46 larva L2    52 larva L3 12hr old    44 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    48 white prepupae new    45 white prepupae 12hr    55 white prepupae 24hr    70 pupae 2d postWPP    71 pupae 3d postWPP    55 pupae 4d postWPP    28 adult male 01day    40 adult male 05day    51 adult male 30day    45 adult female 01day    39 adult female 05day    71 adult female 30day    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    16 embryo 04-06hr    35 embryo 06-08hr    37 embryo 08-10hr    40 embryo 10-12hr    50 embryo 12-14hr    46 embryo 14-16hr    38 embryo 16-18hr    42 embryo 18-20hr    34 embryo 20-22hr    34 embryo 22-24hr    37 larva L1    46 larva L2    52 larva L3 12hr old    44 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    48 white prepupae new    45 white prepupae 12hr    55 white prepupae 24hr    70 pupae 2d postWPP    71 pupae 3d postWPP    55 pupae 4d postWPP    28 adult male 01day    40 adult male 05day    51 adult male 30day    45 adult female 01day    39 adult female 05day    71 adult female 30day    68 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    16 embryo 04-06hr    35 embryo 06-08hr    37 embryo 08-10hr    40 embryo 10-12hr    50 embryo 12-14hr    46 embryo 14-16hr    38 embryo 16-18hr    42 embryo 18-20hr    34 embryo 20-22hr    34 embryo 22-24hr    37 larva L1    46 larva L2    52 larva L3 12hr old    44 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    48 white prepupae new    45 white prepupae 12hr    55 white prepupae 24hr    70 pupae 2d postWPP    71 pupae 3d postWPP    55 pupae 4d postWPP    28 adult male 01day    40 adult male 05day    51 adult male 30day    45 adult female 01day    39 adult female 05day    71 adult female 30day    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (67) embryo 02-04hr    16 embryo 04-06hr  (35) embryo 06-08hr  (37) embryo 08-10hr  (40) embryo 10-12hr  (50) embryo 12-14hr  (46) embryo 14-16hr  (38) embryo 16-18hr  (42) embryo 18-20hr  (34) embryo 20-22hr  (34) embryo 22-24hr  (37) larva L1  (46) larva L2  (52) larva L3 12hr old  (44) larva L3 puffstage 1-2  31 larva L3 puffstage 3-6  (39) larva L3 puffstage 7-9  (48) white prepupae new  (45) white prepupae 12hr  (55) white prepupae 24hr  (70) pupae 2d postWPP  (71) pupae 3d postWPP  (55) pupae 4d postWPP  28 adult male 01day  (40) adult male 05day  (51) adult male 30day  (45) adult female 01day  (39) adult female 05day  (71) adult female 30day  (68) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    16 embryo 04-06hr    35 embryo 06-08hr    37 embryo 08-10hr    40 embryo 10-12hr    50 embryo 12-14hr    46 embryo 14-16hr    38 embryo 16-18hr    42 embryo 18-20hr    34 embryo 20-22hr    34 embryo 22-24hr    37 larva L1    46 larva L2    52 larva L3 12hr old    44 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    48 white prepupae new    45 white prepupae 12hr    55 white prepupae 24hr    70 pupae 2d postWPP    71 pupae 3d postWPP    55 pupae 4d postWPP    28 adult male 01day    40 adult male 05day    51 adult male 30day    45 adult female 01day    39 adult female 05day    71 adult female 30day    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    67 embryo 02-04hr    16 embryo 04-06hr    35 embryo 06-08hr    37 embryo 08-10hr    40 embryo 10-12hr    50 embryo 12-14hr    46 embryo 14-16hr    38 embryo 16-18hr    42 embryo 18-20hr    34 embryo 20-22hr    34 embryo 22-24hr    37 larva L1    46 larva L2    52 larva L3 12hr old    44 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    39 larva L3 puffstage 7-9    48 white prepupae new    45 white prepupae 12hr    55 white prepupae 24hr    70 pupae 2d postWPP    71 pupae 3d postWPP    55 pupae 4d postWPP    28 adult male 01day    40 adult male 05day    51 adult male 30day    45 adult female 01day    39 adult female 05day    71 adult female 30day    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    271 Schneider line 2 Sg4    371 embryonic 1182-4H    151 embryonic GM2    141 embryonic Kc167    437 embryonic S1    181 embryonic S3    174 leg disc CME L1    515 wing disc CME-W2    360 wing disc ML-DmD8    115 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    151 wing disc ML-DmD21    208 wing disc ML-DmD32    190 haltere disc ML-DmD17-c3    122 eye-antennal disc ML-DmD11    211 antennal disc ML-DmD20-c5    209 mixed discs ML-DmD4-c1    139 CNS ML-DmBG1-c1    197 CNS ML-DmBG2-c2    115 tumorous blood cells mbn2    142 ovary fGS/OSS    199 ovary OSC    150 ovary OSS    87 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (271) Schneider line 2 Sg4  (371) embryonic 1182-4H  (151) embryonic GM2  (141) embryonic Kc167  (437) embryonic S1  (181) embryonic S3  (174) leg disc CME L1  (515) wing disc CME-W2  (360) wing disc ML-DmD8  (115) wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (151) wing disc ML-DmD21  (208) wing disc ML-DmD32  (190) haltere disc ML-DmD17-c3  (122) eye-antennal disc ML-DmD11  (211) antennal disc ML-DmD20-c5  (209) mixed discs ML-DmD4-c1  (139) CNS ML-DmBG1-c1  (197) CNS ML-DmBG2-c2  (115) tumorous blood cells mbn2  (142) ovary fGS/OSS  (199) ovary OSC  (150) ovary OSS  (87) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (271) Schneider line 2 Sg4  (371) embryonic 1182-4H  (151) embryonic GM2  (141) embryonic Kc167  (437) embryonic S1  (181) embryonic S3  (174) leg disc CME L1  (515) wing disc CME-W2  (360) wing disc ML-DmD8  (115) wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (151) wing disc ML-DmD21  (208) wing disc ML-DmD32  (190) haltere disc ML-DmD17-c3  (122) eye-antennal disc ML-DmD11  (211) antennal disc ML-DmD20-c5  (209) mixed discs ML-DmD4-c1  (139) CNS ML-DmBG1-c1  (197) CNS ML-DmBG2-c2  (115) tumorous blood cells mbn2  (142) ovary fGS/OSS  (199) ovary OSC  (150) ovary OSS    87 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    271 Schneider line 2 Sg4    371 embryonic 1182-4H    151 embryonic GM2    141 embryonic Kc167    437 embryonic S1    181 embryonic S3    174 leg disc CME L1    515 wing disc CME-W2    360 wing disc ML-DmD8    115 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    151 wing disc ML-DmD21    208 wing disc ML-DmD32    190 haltere disc ML-DmD17-c3    122 eye-antennal disc ML-DmD11    211 antennal disc ML-DmD20-c5    209 mixed discs ML-DmD4-c1    139 CNS ML-DmBG1-c1    197 CNS ML-DmBG2-c2    115 tumorous blood cells mbn2    142 ovary fGS/OSS    199 ovary OSC    150 ovary OSS    87 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    271 Schneider line 2 Sg4    371 embryonic 1182-4H    151 embryonic GM2    141 embryonic Kc167    437 embryonic S1    181 embryonic S3    174 leg disc CME L1    515 wing disc CME-W2    360 wing disc ML-DmD8    115 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    151 wing disc ML-DmD21    208 wing disc ML-DmD32    190 haltere disc ML-DmD17-c3    122 eye-antennal disc ML-DmD11    211 antennal disc ML-DmD20-c5    209 mixed discs ML-DmD4-c1    139 CNS ML-DmBG1-c1    197 CNS ML-DmBG2-c2    115 tumorous blood cells mbn2    142 ovary fGS/OSS    199 ovary OSC    150 ovary OSS    87 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (271) Schneider line 2 Sg4  (371) embryonic 1182-4H  (151) embryonic GM2  (141) embryonic Kc167  (437) embryonic S1  (181) embryonic S3  (174) leg disc CME L1  (515) wing disc CME-W2  (360) wing disc ML-DmD8  (115) wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (151) wing disc ML-DmD21  (208) wing disc ML-DmD32  (190) haltere disc ML-DmD17-c3  (122) eye-antennal disc ML-DmD11  (211) antennal disc ML-DmD20-c5  (209) mixed discs ML-DmD4-c1  (139) CNS ML-DmBG1-c1  (197) CNS ML-DmBG2-c2  (115) tumorous blood cells mbn2  (142) ovary fGS/OSS  (199) ovary OSC  (150) ovary OSS  (87) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (271) Schneider line 2 Sg4  (371) embryonic 1182-4H  (151) embryonic GM2  141 embryonic Kc167  (437) embryonic S1  (181) embryonic S3  (174) leg disc CME L1  (515) wing disc CME-W2  (360) wing disc ML-DmD8  115 wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (151) wing disc ML-DmD21  (208) wing disc ML-DmD32  (190) haltere disc ML-DmD17-c3  122 eye-antennal disc ML-DmD11  (211) antennal disc ML-DmD20-c5  (209) mixed discs ML-DmD4-c1  139 CNS ML-DmBG1-c1  (197) CNS ML-DmBG2-c2  115 tumorous blood cells mbn2  142 ovary fGS/OSS  (199) ovary OSC  (150) ovary OSS    87 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    271 Schneider line 2 Sg4    371 embryonic 1182-4H    151 embryonic GM2    141 embryonic Kc167    437 embryonic S1    181 embryonic S3    174 leg disc CME L1    515 wing disc CME-W2    360 wing disc ML-DmD8    115 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    151 wing disc ML-DmD21    208 wing disc ML-DmD32    190 haltere disc ML-DmD17-c3    122 eye-antennal disc ML-DmD11    211 antennal disc ML-DmD20-c5    209 mixed discs ML-DmD4-c1    139 CNS ML-DmBG1-c1    197 CNS ML-DmBG2-c2    115 tumorous blood cells mbn2    142 ovary fGS/OSS    199 ovary OSC    150 ovary OSS    87 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    58 cold shock, 4-day adult    41 heat shock, 4-day adult    100 Cadmium 50 mM 6 hrs, larvae L3    54 Cadmium 50 mM 12 hrs, larvae L3    57 Cadmium 50 mM 48 hrs, 4-day adult    77 Cadmium 100 mM 48 hrs, 4-day adult    127 Copper 0.5 mM 12 hrs, larvae L3    50 Copper 15 mM 48 hrs, 4-day adult    116 Zinc 5 mM 12 hrs, larvae L3    58 Zinc 4.5 mM 48 hrs, 4-day adult    62 Ethanol 2.5% 3 hrs, larvae L3    110 Ethanol 5% 3 hrs, larvae L3    60 Ethanol 10% 3 hrs, larvae L3    63 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    102 Caffeine 25 mg/ml 48 hrs, 4-day adult    51 Paraquat 5 mM 48 hrs, 4-day adult    85 Paraquat 10 mM 48 hrs, 4-day adult    148 Rotenone 2 μg 12 hrs, larvae L3    63 Rotenone 8 μg 12 hrs, larvae L3    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (58) cold shock, 4-day adult  (41) heat shock, 4-day adult  (100) Cadmium 50 mM 6 hrs, larvae L3  (54) Cadmium 50 mM 12 hrs, larvae L3  (57) Cadmium 50 mM 48 hrs, 4-day adult  (77) Cadmium 100 mM 48 hrs, 4-day adult  (127) Copper 0.5 mM 12 hrs, larvae L3  (50) Copper 15 mM 48 hrs, 4-day adult  (116) Zinc 5 mM 12 hrs, larvae L3  (58) Zinc 4.5 mM 48 hrs, 4-day adult  (62) Ethanol 2.5% 3 hrs, larvae L3  (110) Ethanol 5% 3 hrs, larvae L3  (60) Ethanol 10% 3 hrs, larvae L3  (63) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (42) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (102) Caffeine 25 mg/ml 48 hrs, 4-day adult  (51) Paraquat 5 mM 48 hrs, 4-day adult  (85) Paraquat 10 mM 48 hrs, 4-day adult  (148) Rotenone 2 μg 12 hrs, larvae L3  (63) Rotenone 8 μg 12 hrs, larvae L3  (68) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    58 cold shock, 4-day adult    41 heat shock, 4-day adult    100 Cadmium 50 mM 6 hrs, larvae L3    54 Cadmium 50 mM 12 hrs, larvae L3    57 Cadmium 50 mM 48 hrs, 4-day adult    77 Cadmium 100 mM 48 hrs, 4-day adult  (127) Copper 0.5 mM 12 hrs, larvae L3    50 Copper 15 mM 48 hrs, 4-day adult  (116) Zinc 5 mM 12 hrs, larvae L3    58 Zinc 4.5 mM 48 hrs, 4-day adult    62 Ethanol 2.5% 3 hrs, larvae L3  (110) Ethanol 5% 3 hrs, larvae L3    60 Ethanol 10% 3 hrs, larvae L3    63 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  102 Caffeine 25 mg/ml 48 hrs, 4-day adult    51 Paraquat 5 mM 48 hrs, 4-day adult    85 Paraquat 10 mM 48 hrs, 4-day adult  (148) Rotenone 2 μg 12 hrs, larvae L3    63 Rotenone 8 μg 12 hrs, larvae L3    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    58 cold shock, 4-day adult    41 heat shock, 4-day adult    100 Cadmium 50 mM 6 hrs, larvae L3    54 Cadmium 50 mM 12 hrs, larvae L3    57 Cadmium 50 mM 48 hrs, 4-day adult    77 Cadmium 100 mM 48 hrs, 4-day adult    127 Copper 0.5 mM 12 hrs, larvae L3    50 Copper 15 mM 48 hrs, 4-day adult    116 Zinc 5 mM 12 hrs, larvae L3    58 Zinc 4.5 mM 48 hrs, 4-day adult    62 Ethanol 2.5% 3 hrs, larvae L3    110 Ethanol 5% 3 hrs, larvae L3    60 Ethanol 10% 3 hrs, larvae L3    63 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    102 Caffeine 25 mg/ml 48 hrs, 4-day adult    51 Paraquat 5 mM 48 hrs, 4-day adult    85 Paraquat 10 mM 48 hrs, 4-day adult    148 Rotenone 2 μg 12 hrs, larvae L3    63 Rotenone 8 μg 12 hrs, larvae L3    68 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    58 cold shock, 4-day adult    41 heat shock, 4-day adult    100 Cadmium 50 mM 6 hrs, larvae L3    54 Cadmium 50 mM 12 hrs, larvae L3    57 Cadmium 50 mM 48 hrs, 4-day adult    77 Cadmium 100 mM 48 hrs, 4-day adult    127 Copper 0.5 mM 12 hrs, larvae L3    50 Copper 15 mM 48 hrs, 4-day adult    116 Zinc 5 mM 12 hrs, larvae L3    58 Zinc 4.5 mM 48 hrs, 4-day adult    62 Ethanol 2.5% 3 hrs, larvae L3    110 Ethanol 5% 3 hrs, larvae L3    60 Ethanol 10% 3 hrs, larvae L3    63 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    102 Caffeine 25 mg/ml 48 hrs, 4-day adult    51 Paraquat 5 mM 48 hrs, 4-day adult    85 Paraquat 10 mM 48 hrs, 4-day adult    148 Rotenone 2 μg 12 hrs, larvae L3    63 Rotenone 8 μg 12 hrs, larvae L3    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (58) cold shock, 4-day adult  (41) heat shock, 4-day adult  (100) Cadmium 50 mM 6 hrs, larvae L3  (54) Cadmium 50 mM 12 hrs, larvae L3  (57) Cadmium 50 mM 48 hrs, 4-day adult  (77) Cadmium 100 mM 48 hrs, 4-day adult  (127) Copper 0.5 mM 12 hrs, larvae L3  (50) Copper 15 mM 48 hrs, 4-day adult  (116) Zinc 5 mM 12 hrs, larvae L3  (58) Zinc 4.5 mM 48 hrs, 4-day adult  (62) Ethanol 2.5% 3 hrs, larvae L3  (110) Ethanol 5% 3 hrs, larvae L3  (60) Ethanol 10% 3 hrs, larvae L3  (63) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (42) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (102) Caffeine 25 mg/ml 48 hrs, 4-day adult  (51) Paraquat 5 mM 48 hrs, 4-day adult  (85) Paraquat 10 mM 48 hrs, 4-day adult  (148) Rotenone 2 μg 12 hrs, larvae L3  (63) Rotenone 8 μg 12 hrs, larvae L3  (68) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    58 cold shock, 4-day adult    41 heat shock, 4-day adult    100 Cadmium 50 mM 6 hrs, larvae L3    54 Cadmium 50 mM 12 hrs, larvae L3    57 Cadmium 50 mM 48 hrs, 4-day adult    77 Cadmium 100 mM 48 hrs, 4-day adult  127 Copper 0.5 mM 12 hrs, larvae L3    50 Copper 15 mM 48 hrs, 4-day adult  116 Zinc 5 mM 12 hrs, larvae L3    58 Zinc 4.5 mM 48 hrs, 4-day adult    62 Ethanol 2.5% 3 hrs, larvae L3  110 Ethanol 5% 3 hrs, larvae L3    60 Ethanol 10% 3 hrs, larvae L3    63 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  102 Caffeine 25 mg/ml 48 hrs, 4-day adult    51 Paraquat 5 mM 48 hrs, 4-day adult    85 Paraquat 10 mM 48 hrs, 4-day adult  (148) Rotenone 2 μg 12 hrs, larvae L3    63 Rotenone 8 μg 12 hrs, larvae L3    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    58 cold shock, 4-day adult    41 heat shock, 4-day adult    100 Cadmium 50 mM 6 hrs, larvae L3    54 Cadmium 50 mM 12 hrs, larvae L3    57 Cadmium 50 mM 48 hrs, 4-day adult    77 Cadmium 100 mM 48 hrs, 4-day adult    127 Copper 0.5 mM 12 hrs, larvae L3    50 Copper 15 mM 48 hrs, 4-day adult    116 Zinc 5 mM 12 hrs, larvae L3    58 Zinc 4.5 mM 48 hrs, 4-day adult    62 Ethanol 2.5% 3 hrs, larvae L3    110 Ethanol 5% 3 hrs, larvae L3    60 Ethanol 10% 3 hrs, larvae L3    63 Caffeine 1.5 mg/ml 4 hrs, larvae L3    42 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    102 Caffeine 25 mg/ml 48 hrs, 4-day adult    51 Paraquat 5 mM 48 hrs, 4-day adult    85 Paraquat 10 mM 48 hrs, 4-day adult    148 Rotenone 2 μg 12 hrs, larvae L3    63 Rotenone 8 μg 12 hrs, larvae L3    68 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_26 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • GH10148
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die | embryonic stage | maternal effect mbf12 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 mbf1GD4736 viable mbf11 mbf12 mbf13 mbf14 Sterility Allele fertile mbf11 mbf12 mbf13 mbf14 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 mbf1GD4736 chemical resistant mbf1+t4574 stress response defective mbf12 stress response defective (with Df(3L)st7P) mbf12 visible, with Scer\GAL4pnr-MD237 mbf1GD4736 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 mbf1GD4736 presumptive embryonic/larval central nervous system mbf12 presumptive embryonic/larval tracheal system mbf12 tormogen cell, with Scer\GAL4pnr-MD237 mbf1GD4736 trichogen cell, with Scer\GAL4pnr-MD237 mbf1GD4736
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of mbf1 Class Mutagen Stocks Known lesion mbf1f05959 piggyBac transposase 1 -- mbf1f07605 piggyBac transposase 1 -- mbf11 P-element activity 0 Yes mbf12 P-element activity 0 Yes mbf13 P-element activity 0 Yes mbf14 P-element activity 0 Yes mbf1EP3684 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mbf1 Class Mutagen Stocks Known lesion mbf1GD4736 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes mbf1HM05036 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbf1KK109070 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbf1+t4574 in vitro construct - genomic fragment 0 Yes mbf1Hsp83.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)st7P (Jindra, 1999.3.12)
Not disrupted in	Df(3L)st-e4 (Jindra, 1999.3.12)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hs-mbf1::FLAG} NA UAS construct P{GD4736} NA P{KK109070} NA P{TRiP.HM05036} NA characterization construct P{mbf1+t4574} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}mbf1EP3684 NA miscellaneous insertions P{}mbf1P1 NA PBac{WH}mbf1f05959 NA PBac{WH}mbf1f07605 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
protein binding, bridging inferred from physical interaction with apt, Tbp (Liu et al., 2003) transcription coactivator activity inferred from direct assay (Liu et al., 2003)
Biological Process
CV term Evidence References
central nervous system development inferred from genetic interaction with apt AND inferred from mutant phenotype (Liu et al., 2003) dendrite morphogenesis inferred from mutant phenotype (Parrish et al., 2006) open tracheal system development inferred from genetic interaction with apt AND inferred from mutant phenotype (Liu et al., 2003) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Liu et al., 2003)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Jindra et al., 2004) nucleus inferred from direct assay (Jindra et al., 2004)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
methyl-CpG binding inferred from sequence or structural similarity with UniProtKB:Q9Z2E1 (FlyBase, 1992-) transcription coactivator activity inferred from sequence or structural similarity with Saccharomyces MBF1 (FlyBase, 1992-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_stop_codon_read_through (Jungreis et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
mbf1 - CrebA anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mbf1 allele Gene References mbf12 apt (Liu et al., 2003) Jra (Jindra et al., 2004) kay (Jindra et al., 2004)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262732
Orthologs
OrthoDB Orthologs (37) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG64MZCN Dipteran inclusive ortholog search EOG637RQ8 Insect inclusive ortholog search EOG6C2G72 Arthropod inclusive ortholog search EOG6WPZK3 Metazoa inclusive ortholog search EOG686N7N
Orthologs in Drosophila Species (EOG64MZCN)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\mbf1     Drosophila simulans   Dsim\GD14645 Y   Drosophila sechellia   Dsec\GM25642 Y   Drosophila erecta   Dere\GG13561 Y   Drosophila yakuba   Dyak\mbf1 Y   Drosophila ananassae   Dana\GF23918 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17985 Y   Drosophila persimilis   Dper\GL17867 Y   Drosophila willistoni   Dwil\GK19781 Y   Drosophila virilis   Dvir\GJ12924 Y   Drosophila mojavensis   Dmoj\GI16673 Y   Drosophila grimshawi   Dgri\GH15018 Y
Orthologs in non-Drosophila Dipterans (EOG637RQ8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008768   Anopheles gambiae Malaria mosquito  Agam\AGAP004990   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003986
Orthologs in non-Dipteran Insects (EOG6C2G72)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB48171   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007203   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13394   Atta cephalotes Leafcutter ant  Acep\ACEP24798   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22240   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15833   Linepithema humile Argentine ant  Lhum\LH21699   Pogonomyrmex barbatus Red harvester ant  Pbar\PB13205   Solenopsis invicta Red fire ant  Sinv\SINV24909   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005677   Bombyx mori Silkmoth  Bmor\BGIBMGA007702   Pediculus humanus Human body louse  Phum\PHUM132170   Tribolium castaneum Red flour beetle  Tcas\TC000104
Orthologs in non-Insect Arthropods (EOG6WPZK3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_209727   Ixodes scapularis Deer tick  Isca\ISCW022655
Orthologs in non-Arthropod Metazoa (EOG686N7N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\mbf-1   Danio rerio Zebrafish  Drer\edf1   Xenopus tropicalis Western clawed frog  Xtro\edf1   Gallus gallus Domestic chicken  Ggal\EDF1   Mus musculus House mouse  Mmus\Edf1   Rattus norvegicus Norway rat  Rnor\Edf1   Homo sapiens Human  Hsap\ENSG00000107223
Human Orthologs (1)
Gene OMIM HGNC Hsap\ENSG00000107223
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD14645 Drosophila sechellia Dsec\GM25642 Drosophila erecta Dere\GG13561 Drosophila yakuba Dyak\mbf1 Drosophila ananassae Dana\GF23918 Drosophila pseudoobscura pseudoobscura Dpse\GA17985 Drosophila persimilis Dper\GL17867 Drosophila willistoni Dwil\GK19781 Drosophila virilis Dvir\GJ12924 Drosophila mojavensis Dmoj\GI16673 Drosophila grimshawi Dgri\GH15018
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	28550 y1 v1; P{TRiP.HM05036}attP2
Harvard	- PBac{WH}mbf1f05959 - PBac{WH}mbf1f07605
VDRC	v107193 P{KK109070}VIE-260B v12751 w1118; P{GD4736}v12751 v12752 w1118; P{GD4736}v12752
Genomic Clones ( 1 )
	BACR08H06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 72 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH10148 GM01267
Other clones	DPiM_GH10148 yak-ad_mbf1
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT17183 AT21410 GH07649 LP14364 RE03242 RE19407 RE37861 RE52170 RH02670 RH03305 RH03933 RH03971 RH04263 RH04276 RH44470 RH46945 RH56404 RH73643 SD12494 SD19290 SD22276 SD25335 SD27671
Other clones	15092 30549 43334 43398 73094 104738 105183 149855 162002 214215.43 280553 282552 bs38e02 CK01.15A.D3 CK01.39C.B10 CK01.82B.C4 CK01.87C.C11 CK01.94C.F7 CK01.100B.A6 CK01.100B.C4 CK01.109D.D5 Dmel_neb_012268_0691_3630 EC13774 EK064942 EK073624 EK077235 EK084827 EK084832 EK099115 EK118259 EK139427 EK156401 EK162730 EK230212 EK266323 EK303508 EK305439 EK307857 EP12612 FGM308E06 LD23791 RP003250712 RP003296740 UUGC0454 UUGC0482
RNAi & Array Information
Linkouts	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39842
Antibody Information
	polyclonal (Jindra et al., 2004)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: mbf1 BcDNA:GH10148 (FlyBase, 1996-) Source for merge of: mbf1 BcDNA:GM01267 (Rana, 2004.7.2) Source for merge of: mbf1 MRE4 (FlyBase Genome Annotators, 2011)
Additional comments	MRE4 is part of the 3' UTR of mbf1. (FlyBase Genome Annotators, 2011) MRE4 corresponds to a portion of the 3' UTR of mbf1. (FlyBase Genome Annotators, 2011) Source for merge of mbf1 BcDNA:GH10148 was sequence comparison (date:000129). (FlyBase, 1996-)
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. RNAi of mbf1 results in dorsal overextension of primary dendrites, defects in dendrite morphogenesis and a reduction in lateral branching. (Parrish et al., 2006) mbf1 protein binds the basic region of Jra protein and protects the critical cysteine residue (which controls in part the activity of the AP-1 (Jra,kay) heterodimer) from oxidation. mbf1 allows cells to maintain adequate AP-1 activity under oxidative stress. (Jindra et al., 2004)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079386 NM_168673 NM_001259866 NM_001259867 NM_001275023 RefSeq (Proteins) • NP_524110 NP_730178 NP_001246795 NP_001246796 NP_001261952 DDBJ / EMBL / GenBank • AA694681 AAD34744 AAF49449 AAM49830 AAN11755 AB031273 AF132156 AFH04466 AFH04467 AGB94645 AI113713 AQ073116 AW940346 AW941127 AY118461 BAA83523 CZ488585 CZ488586 CZ490351 Entrez Gene - A searchable database of RefSeq genes. • 39842 UniProtKB/TrEMBL • Q8MT00 Q9XZ03
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Helix-turn-helix (IPR001387) Lambda repressor-like, DNA-binding domain (IPR010982) Multiprotein bridging factor 1, N-terminal (IPR013729) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH10148
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262732 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39842
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	BcDNA:GH10148   BcDNA:GM01267   CG4143 (Qin et al., 2005, Keleman et al., 2009.8.5, Perkins et al., 2009, Awofala et al., 2012) mbf1 (Arancio et al., 2010, Jungreis et al., 2011, FlyBase Genome Annotators, 2011, Qin et al., 2005, Birch-Machin et al., 2005, Burgio et al., 2008, Kong et al., 2010, Awofala et al., 2012) MBF-1 (Roignant et al., 2003) MBF1 (Faucheux et al., 2003, Jindra et al., 2004, Liu et al., 2000, Jindra et al., 2005, Rynes and Jindra, 2007, Rynes and Jindra, 2007) Mbf1 (Parrish et al., 2006) MRE4 (FlyBase Genome Annotators, 2011, Kageyama, 2007.2.28, Inagaki et al., 2005)
Name Synonym	mRNA-like ncRNA in embryogenesis 4   multiprotein bridging factor 1 (Jindra et al., 1999, ) Multiprotein bridging factor 1 (Rynes and Jindra, 2007, Qin et al., 2005, Awofala et al., 2012)
Secondary FlyBase IDs
	FBgn0026208 FBgn0027555 FBgn0063210 FBgn0086536
References ( 40 )
Generate a list of	All references relating to this gene ( 40 )
List References by type	Research paper  ( 12 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 7 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 )
Recent research papers ( 1 )
	Awofala et al., 2012, Funct. Integr. Genomics 12(2): 305--315 Functional roles for redox genes in ethanol sensitivity in Drosophila. [FBrf0218408] Show all research papers ...