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General Information
Symbol
Dmel\Src64B
Species
D. melanogaster
Name
Src oncogene at 64B
Annotation Symbol
CG7524
Feature Type
FlyBase ID
FBgn0262733
Gene Model Status
Stock Availability
Enzyme Name (EC)
Non-specific protein-tyrosine kinase (2.7.10.2)
Gene Snapshot
Src oncogene at 64B (Src64B) encodes a Src family nonreceptor tyrosine kinase. It has many biological roles, including ring canal morphogenesis in oogenesis and the male germline, microfilament ring constriction during cellularization, and modulation of growth and apoptosis. Some of its roles overlap with those of the product of Src42A. [Date last reviewed: 2019-03-14]
Also Known As

Src64, Src, Dsrc64B, Src1, MRE5

Key Links
Genomic Location
Cytogenetic map
Sequence location
3L:4,592,447..4,626,196 [-]
Recombination map

3-12

RefSeq locus
NT_037436 REGION:4592447..4626196
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. (P00528)
Catalytic Activity (EC)
Experimental Evidence
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (2.7.10.2)
Predictions / Assertions
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (2.7.10.2)
Summaries
Gene Group (FlyBase)
NON-RECEPTOR TYROSINE KINASES -
Non-Receptor Tyrosine kinases (nRTK) are cytoplasmic protein kinases that specifically phosphorylate tyrosine residues. nRTKs are involved in signalling cascades and possess targeting domains such as SH2 domains. (Adapted from FBrf0132098).
Pathway (FlyBase)
Positive Regulators of Torso Signaling Pathway -
Positive regulators of Torso signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl).
Positive Regulators of Sevenless Signaling Pathway -
Positive regulators of Sevenless signaling up-regulate the pathway, promoting cell fate to that of an R7 photoreceptor. (Adapted from FBrf0127283 and FBrf0221727).
Positive Regulators of Epidermal Growth Factor Receptor Signaling Pathway -
Positive regulators of Epidermal Growth Factor Receptor signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl) or acting on downstream effectors.
Positive Regulators of Fibroblast Growth Factor Receptor Signaling Pathway -
Positive regulators of Fibroblast Growth Factor Receptor signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl) or acting on other effectors.
Protein Function (UniProtKB)
May play a role in the development of neural tissue and smooth muscle.
(UniProt, P00528)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Src1: Src proto oncogene sequence
Considered to be the Drosophila sequence homologous to mammalian c-src, based both on its origin and amino acid sequence as inferred from its nucleotide sequence. The polypeptide product as yet uncharacterized, but presumed to be a protein kinase; Drosophila extracts do exhibit tyrosine kinase activity (Simon et al., 1983). In the region of the polypeptide responsible for kinase and transforming activity, Drosophila amino acid sequence 54% homologous with that from v-src, 100 base pairs beginning with the tryosine-416 codon 62% homologous with Drosophila Abl (Hoffman, Fresco, Hoffman-Falk, and Shilo, 1983, Cell 35: 393-401). Src transcripts abundant in early embryos and in adult ovaries. Level declines in later embryos and is low in males and ovarectomized females; inferred to be a maternally acting gene whose product is required for early embryogenesis (Wadsworth, Madhaven, and Bilodeau-Wentworth, 1985, Nucleic Acids Res. 13: 2153-70).
Summary (Interactive Fly)

Src homolog: a protein tyrosine kinase with SH3 and SH2 domains - plays a role in ring canal morphogenesis during oogenesis and the male germline, microfilament ring constriction during cellularization, and modulation of growth and apoptosis. Some of its roles overlap with those of Src42A.

Gene Model and Products
Number of Transcripts
9
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Src64B or the JBrowse view of Dmel\Src64B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.53

Gene model reviewed during 6.01

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0073321
2782
552
FBtr0100504
2567
552
FBtr0100505
2815
552
FBtr0100507
2717
552
FBtr0100508
2956
552
FBtr0302594
2510
552
FBtr0304989
4338
552
FBtr0304990
4786
552
FBtr0343995
4406
553
Additional Transcript Data and Comments
Reported size (kB)

4.8, 4.4, 3.0 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0073177
63.0
552
7.15
FBpp0099944
63.0
552
7.15
FBpp0099946
63.0
552
7.15
FBpp0099947
63.0
552
7.15
FBpp0099948
63.0
552
7.15
FBpp0291750
63.0
552
7.15
FBpp0293526
63.0
552
7.15
FBpp0293527
63.0
552
7.15
FBpp0310444
63.1
553
6.98
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Src64B using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (40 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN002521528
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002521528
(assigned by GO_Central )
Biological Process (33 terms)
Terms Based on Experimental Evidence (29 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002521528
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002521528
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002521528
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002521528
(assigned by GO_Central )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN002521528
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
dot blot
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Src64B protein is localized to the cellularization front during cellularization of the embryo.

Src64B protein is detected in ring canals and nurse cell membranes.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Src64B in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 47 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Src64B
Transgenic constructs containing regulatory region of Src64B
Deletions and Duplications ( 10 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
actin filament & nurse cell ring canal
alpha-lobe & axon
beta-lobe & axon
mushroom body & axon, with Scer\GAL47B
nurse cell & nucleus
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (34)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
 
9 of 15
Yes
Yes
8 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
6 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
No
5 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
 
2 of 15
No
No
 
2 of 15
No
No
2 of 15
No
No
 
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
 
 
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (33)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
8 of 15
No
Yes
8 of 15
No
Yes
8 of 15
No
Yes
6 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
No
5 of 15
No
Yes
5 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (33)
8 of 13
Yes
Yes
8 of 13
Yes
Yes
8 of 13
Yes
Yes
7 of 13
No
Yes
6 of 13
No
Yes
6 of 13
No
Yes
6 of 13
No
Yes
5 of 13
No
Yes
4 of 13
No
No
3 of 13
No
No
3 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
2 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (29)
6 of 12
Yes
Yes
6 of 12
Yes
Yes
5 of 12
No
Yes
5 of 12
No
Yes
4 of 12
No
Yes
3 of 12
No
Yes
2 of 12
No
No
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (43)
9 of 15
Yes
Yes
8 of 15
No
Yes
8 of 15
No
Yes
8 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
Yes
5 of 15
No
Yes
5 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (9)
10 of 15
Yes
Yes
5 of 15
No
No
4 of 15
No
Yes
2 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (17)
3 of 9
Yes
Yes
2 of 9
No
Yes
2 of 9
No
Yes
1 of 9
No
No
1 of 9
No
Yes
1 of 9
No
No
1 of 9
No
Yes
1 of 9
No
No
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
No
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
No
1 of 9
No
Yes
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (2)
1 of 15
Yes
No
1 of 15
Yes
No
Schizosaccharomyces pombe (Fission yeast) (1)
1 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919052W )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915052W )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00MF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04UG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0D46 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (13)
7 of 10
3 of 10
3 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 2 )
Modifiers Based on Experimental Evidence ( 2 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Dmel gene
Ortholog showing functional complementation
Supporting References
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
suppressible
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
suppressible
External Data
Linkouts
DroID - A comprehensive database of gene and protein interactions.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Positive Regulators of Torso Signaling Pathway -
Positive regulators of Torso signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl).
Positive Regulators of Sevenless Signaling Pathway -
Positive regulators of Sevenless signaling up-regulate the pathway, promoting cell fate to that of an R7 photoreceptor. (Adapted from FBrf0127283 and FBrf0221727).
Positive Regulators of Epidermal Growth Factor Receptor Signaling Pathway -
Positive regulators of Epidermal Growth Factor Receptor signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl) or acting on downstream effectors.
Positive Regulators of Fibroblast Growth Factor Receptor Signaling Pathway -
Positive regulators of Fibroblast Growth Factor Receptor signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl) or acting on other effectors.
Metabolic Pathways
External Data
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3L
Recombination map

3-12

Cytogenetic map
Sequence location
3L:4,592,447..4,626,196 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
64B11-64B12
Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
64B-64B
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (33)
Genomic Clones (44)
cDNA Clones (221)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of
Source for database merge of

Source for merge of: Src64B CG7524

Source for merge of: Src64B MRE5

Additional comments

MRE5 is part of the 3' UTR of Src64B.

MRE5 corresponds to a portion of the 3' UTR of Src64B.

Identified by PCR fragment; relationship to other protein tyrosine kinase genes not known.

Other Comments

Src64B expression induces JNK-mediated apoptosis or tissue overgrowth when apoptosis is blocked with p35.

Duplicate transcripts identified and eliminated during the migration of annotations from the release 5 genome assembly to the release 6 assembly.

Based on the frequency of observed ovarian defects, the following Src64B alleles can be ranked from strongest to weakest as follows: Src64BΔ17 > Src64BPI > Src64BΔ19 > Src64BKG00213.

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G2/M phase cells, a whole range of mitotic abnormalities, spindle abnormalities and chromosome abnormalities are seen.

Src64B plays a role in the cytoskeletal dynamics that occur during cellularization of the blastoderm.

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

Clonal analysis with Src64BPI shows that the requirement for Src64B in mushroom body beta-lobe development is cell autonomous.

Src64B is essential for the proper organization of actin in ovarian ring canals.

Src64B is required for the proper growth and stability of ovarian ring canals.

Phylogenetic analysis of the PTK family.

Abnormal eye development caused by ectopic expression of an activated form of Src64B is alleviated by dominant-negative mutations of csw or drk.

The closest relative of vertebrate c-src so far found is not Src64B, but Src42A.

Phosphorylation of the C-terminal tail of the Src64B protein can repress the activity of the adjacent catalytic domain of the protein.

Considered to be the Drosophila sequence homologous to mammalian c-src, based both on its origin and amino acid sequence as inferred from its nucleotide sequence.

Src64B inferred to be a maternally acting gene whose product is required for early embryogenesis.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 117 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
SignaLink - A signaling pathway resource with multi-layered regulatory networks.
Linkouts
DroID - A comprehensive database of gene and protein interactions.
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (35)
Reported As
Symbol Synonym
C-src1
HD-358
Src64B
(Meltzer et al., 2019, Moreno et al., 2019, Nil et al., 2019, Tsarouhas et al., 2019, Arbeille and Bashaw, 2018, Enomoto et al., 2018, Olesnicky and Wright, 2018, Poon et al., 2018, Richardson and Portela, 2018, Das and Cagan, 2017, Houtz et al., 2017, Hu et al., 2017.6.13, Neuert et al., 2017, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Hoi et al., 2016, Öztürk-Çolak et al., 2016, Spring et al., 2016, Enomoto et al., 2015, Hsu et al., 2015, Kavi et al., 2015, Luo et al., 2015, Morgante et al., 2015, Turkel et al., 2015, Ashwal-Fluss et al., 2014, Bischof and FlyORF project members, 2014.6.20, Bülow et al., 2014, Fernández et al., 2014, FlyBase Genome Annotators, 2014, Macagno et al., 2014, Sopko et al., 2014, Tsarouhas et al., 2014, Tutor et al., 2014, Carter, 2013, Ma et al., 2013, Muha and Müller, 2013, O'Donnell and Bashaw, 2013, Pechkovsky et al., 2013, Ríos-Barrera and Riesgo-Escovar, 2013, Sopko and Perrimon, 2013, Sotillos et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Nelson et al., 2012, FlyBase Genome Annotators, 2011, Aerts et al., 2010, Csiszar et al., 2010, Singh et al., 2010, Hazelett et al., 2009, Langton et al., 2009, Tan et al., 2009, Williams, 2009, Berger et al., 2008, Ranade et al., 2008, Shindo et al., 2008, Song et al., 2008, Tsai et al., 2008, Wouda et al., 2008, Xia et al., 2008, Bianco et al., 2007, Gregory et al., 2007, Junion et al., 2007, Langton et al., 2007, Maines et al., 2007, Quinones-Coello, 2007, Jordan et al., 2005, MacDougall et al., 2004)
src1
Name Synonyms
Src oncogene at 64B
Src proto oncogene sequence
mRNA-like ncRNA in embryogenesis 5
Secondary FlyBase IDs
  • FBgn0003501
  • FBgn0022792
  • FBgn0035561
  • FBgn0086537
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (257)