Gene Dmel\Src64B

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Tyrosine-protein kinase, non-receptor Src64B (IPR020703)

Protein kinase, catalytic domain (IPR000719)

SH2 motif (IPR000980)

Src homology-3 domain (IPR001452)

Tyrosine-protein kinase, active site (IPR008266)

Protein kinase-like domain (IPR011009)

Protein kinase, ATP binding site (IPR017441)

Tyrosine-protein kinase, catalytic domain (IPR020635)

Serine-threonine/tyrosine-protein kinase (IPR001245)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P00528

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

dot blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

prepupal stage -- pupal stage

adult stage | female

oogenesis & adult stage | female

ovary

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage & oogenesis

oocyte

(Kimchie et al., 1989)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Notes

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 5

furrow canal | restricted

adult stage & oogenesis

nurse cell | restricted

(Roulier et al., 1998)

ring canal

(Roulier et al., 1998)

Additional Descriptive Data

Src64B protein is localized to the cellularization front during cellularization of the embryo.

(The modENCODE Consortium, 2010)

Src64B protein is detected in ring canals and nurse cell membranes.

(Roulier et al., 1998)

Marker for

Subcellular Localization

CV Term

Notes

High-Throughput Expression Data

Untitled Document detailed view

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

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modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

mE2_34_mRNA_expression_cluster_17

External Data & Images

female semi-sterile | recessive

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^GMR.PF

Src64B^YF.Scer\UAS

lethal | embryonic stage | maternal effect | recessive

Src64B^Δ17

lethal | heat sensitive

lethal | partially | heat sensitive

Src64B^KR.hs.2sev

viable

viable, with Scer\GAL4^pnr-MD237

Src64B^GD12263

Sterility

Allele

female sterile | partially | recessive

fertile

fertile | poor

Src64B^Δ17

male fertile

Other Phenotypes

Allele

cell shape defective

Src64B^Δ17

increased cell death

Src64B^Δ17

increased cell death, with Scer\GAL4^GMR.PF

Src64B^Scer\UAS.cPa

memory defective

Src64B^chingis-khan

mitotic cell cycle defective, with Scer\GAL4^GMR.PF

neuroanatomy defective

Src64B^PI

neuroanatomy defective, with Scer\GAL4^7B

neuroanatomy defective, with Scer\GAL4^ey-OK107

visible

Phenotype manifest in

Allele

actin filament

Src64B^Δ17

actin filament & nurse cell ring canal

Src64B^Δ17

actin filament | embryonic stage 5

Src64B^Δ17

alpha-lobe & axon

Src64B^PI

anterior commissure | heat sensitive

Src64B^KR.hs.2sev

beta-lobe & axon

Src64B^PI

border follicle cell, with Scer\GAL4^slbo.2.6

Src64B^Scer\UAS.cCa

centripetally migrating follicle cell, with Scer\GAL4^slbo.2.6

Src64B^Scer\UAS.cCa

denticle belt | heat sensitive

Src64B^{Δ540.hs.2sev}

egg

Src64B^PI

egg | maternal effect

Src64B^Δ17

egg chamber

egg chamber, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

Src64B^YF.Scer\UAS

egg chamber (with Src64B^ko)

Src64B^Δ17

egg chamber (with Src64B^Δ17)

Src64B^ko

egg chamber | germline clone

embryonic/first instar larval cuticle | heat sensitive

Src64B^{Δ540.hs.2sev}

extended germ band embryo | heat sensitive

eye, with Scer\GAL4^GMR.PF

eye | heat sensitive

eye photoreceptor cell | heat sensitive

filzkorper | heat sensitive

Src64B^{Δ540.hs.2sev}

furrow canal

Src64B^Δ17

germline cyst

germline cyst, with Scer\GAL4^{nos.UTR.T:Hsim\VP16}

Src64B^YF.Scer\UAS

inner nurse cell ring canal rim

Src64B^PI

interfollicle cell

Src64B^Δ17

interommatidial bristle | ectopic, with Scer\GAL4^GMR.PF

Src64B^Scer\UAS.cPa

karyosome

Src64B^Δ17

Kenyon cell, with Scer\GAL4^7B

Kenyon cell, with Scer\GAL4^ey-OK107

longitudinal connective | heat sensitive

mushroom body

Src64B^PI

mushroom body & axon, with Scer\GAL4^7B

mushroom body & axon, with Scer\GAL4^ey-OK107

mushroom body alpha-lobe, with Scer\GAL4^7B

mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

mushroom body beta-lobe, with Scer\GAL4^7B

mushroom body beta-lobe, with Scer\GAL4^ey-OK107

nurse cell

nurse cell & nucleus

Src64B^Δ17

nurse cell fusome

Src64B^Δ17

nurse cell ring canal

nurse cell ring canal | maternal effect

Src64B^Δ17

ommatidium | heat sensitive

oocyte

outer nurse cell ring canal rim

Src64B^PI

photoreceptor cell R7

Src64B^Δ540.hs.sev

plasma membrane | embryonic stage 5

Src64B^Δ17

posterior commissure | heat sensitive

Src64B^KR.hs.2sev

presumptive embryonic/larval nervous system | heat sensitive

presumptive embryonic salivary gland

Src64B^PI

rhabdomere | heat sensitive

Src64B^KR.hs.2sev

Classical Alleles ( 33 )

For All Classical Alleles Show

Allele of Src64B	Class	Mutagen	Stocks	Known lesion
Src64B^c04709		piggyBac transposase	2	--
Src64B^5-SZ-3243			1	--
Src64B^c01424		piggyBac transposase	1	--
Src64B^CB-6665-3			1	--
Src64B^d05355		P-element activity	1	--
Src64B^DG14509		P-element activity	1	--
Src64B^e00290		piggyBac transposase	1	--
Src64B^EY16432		P-element activity	1	--
Src64B^f05012		piggyBac transposase	1	--
Src64B^f07626		piggyBac transposase	1	--
Src64B^G7151		P-element activity	1	--
Src64B^GS9618			1	Yes
Src64B^KG00213		P-element activity	1	--
Src64B^MB03494			1	--
Src64B^MI00693			1	--
Src64B^PI.D		ethyl methanesulfonate	1	Yes
Src64B^PI	hypomorphic allele - genetic evidence	Delta2-3	1	Yes
Src64B^UY1332		P-element activity	1	Yes
Src64B^ko	amorphic allele - genetic evidence	recombination SCEI endonuclease FLPase	0	Yes
Src64B^BGT-T063		P-element activity	0	Yes
Src64B^c01746		piggyBac transposase	0	--
Src64B^c06319		piggyBac transposase	0	--
Src64B^chingis-khan		P-element activity	0	--
Src64B^CPTI000401		piggyBac transposase	0	Yes
Src64B^CPTI000567		piggyBac transposase	0	Yes
Src64B^d05393		P-element activity	0	--
Src64B^e01280			0	--
Src64B^EP3672		P-element activity	0	--
Src64B^EY12445		P-element activity	0	--
Src64B^f05059		piggyBac transposase	0	--
Src64B^f06927		piggyBac transposase	0	--
Src64B^Δ17	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
Src64B^Δ19		Delta2-3	0	Yes

Alleles Carried on Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Src64B	Mutagen	Stocks	Known lesion
Src64B^GD12263	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Src64B^GL00297	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Src64B^JF03234	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Src64B^Scer\UAS.cCa	in vitro construct - regulatory fusion	1	Yes
Src64B^Act5C.PG	in vitro construct - deletion in vitro construct - regulatory fusion in vitro construct	0	Yes
Src64B^Act5C.PW	in vitro construct - regulatory fusion	0	Yes
Src64B^dsRNA.cWa	in vitro construct - RNAi	0	Yes
Src64B^hs.2sev	in vitro construct - regulatory fusion	0	Yes
Src64B^hs.PD	in vitro construct - regulatory fusion	0	Yes
Src64B^K312R	in vitro construct - amino acid replacement	0	Yes
Src64B^{ko.Scer\SceI.RS}	in vitro construct	0	Yes
Src64B^KR.hs.2sev	in vitro construct - regulatory fusion	0	Yes
Src64B^osk.PO	in vitro construct - regulatory fusion	0	Yes
Src64B^Scer\UAS.cPa	in vitro construct - regulatory fusion	0	Yes
Src64B^Y434F	in vitro construct - amino acid replacement	0	Yes
Src64B^Y547F	in vitro construct - amino acid replacement	0	Yes
Src64B^YF.Scer\UAS	in vitro construct - regulatory fusion	0	Yes
Src64B^αTub67C.PD	in vitro construct - regulatory fusion	0	Yes
Src64B^{Δ540.hs.2sev}	in vitro construct - regulatory fusion	0	Yes
Src64B^Δ540.hs.sev	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Not disrupted in

Df(3L)GN19

Df(3L)GN34

Df(3L)HR277

Df(3L)HR298

Df(3L)TE1

Df(3L)TE3

Df(3L)X37

T(2;3)TE2

Disrupted in

Df(3L)ems13

P{FRT(w^+mW.hs.Src64B.Scer\SceI.RS</up>)}

Df(3L)Exel7208

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

heat-shock construct

UAS construct

characterization construct

P{Ras^Myr}

P{SE-csw^C583Ssrc90}

P{sevhs-csw^src90}

P{sevhs-csw^src90G2A}

P{sev-Src90-Ras1^V12.ΔCAAX}

P{UAS-csw.src90}

P{UAS-unc-5.Myr}

P{αTub67C-Src64B.D}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}Rpd3^EP3672

P{EP}Src64B^G7151

P{EPgy2}Src64B^EY12445

P{EPgy2}Src64B^EY16432

P{GSV6}Src64B^GS9618

P{Mae-UAS.6.11}Src64B^UY1332

P{XP}Src64B^d05355

P{XP}Src64B^d05393

miscellaneous insertions

Mi{MIC}Src64B^MI00693

P{lwB.D}Src64B^PI.D

P{lwB.D}Src64B^PI

P{RS3}Src64B^CB-6665-3

P{RS5}Src64B^5-SZ-3243

P{SUPor-P}Src64B^KG00213

P{wHy}Src64B^DG14509

PBac{602.P.SVS-1}Src64B^CPTI000401

PBac{602.P.SVS-1}Src64B^CPTI000567

PBac{PB}Src64B^c01424

PBac{PB}Src64B^c01746

PBac{PB}Src64B^c04709

PBac{PB}Src64B^c06319

PBac{RB}Src64B^e00290

PBac{RB}Src64B^e01280

PBac{WH}Src64B^f05012

PBac{WH}Src64B^f05059

PBac{WH}Src64B^f06927

PBac{WH}Src64B^f07626

insertion of enhancer trap

P{lacZ}Src64B^chingis-khan

P{lwB}Src64B^BGT-T063

insertion of enhancer trap binary system

Mi{ET1}Src64B^MB03494

Gene Ontology: Function, Process & Cellular Component ( 24 unique terms )

Terms Based on Experimental Evidence ( 20 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

actin cytoskeleton organization

References

inferred from mutant phenotype

cellularization

inferred from mutant phenotype

female germline ring canal formation

inferred from mutant phenotype

(Dodson et al., 1998, O'Reilly et al., 2006)

female germline ring canal formation, actin assembly

inferred from mutant phenotype

female germline ring canal stabilization

inferred from mutant phenotype

(Dodson et al., 1998)

germarium-derived egg chamber formation

inferred from mutant phenotype

(O'Reilly et al., 2006)

germarium-derived female germ-line cyst encapsulation

inferred from mutant phenotype

(O'Reilly et al., 2006)

karyosome formation

inferred from mutant phenotype

mushroom body development

learning or memory

inferred from mutant phenotype

(Dubnau et al., 2003)

inferred from mutant phenotype

(Nicolai et al., 2003)

olfactory learning

inferred from mutant phenotype

(Dubnau et al., 2003)

oogenesis

inferred from mutant phenotype

ovarian fusome organization

inferred from mutant phenotype

ovarian nurse cell to oocyte transport

inferred from mutant phenotype

parallel actin filament bundle assembly

inferred from mutant phenotype

peptidyl-tyrosine phosphorylation

inferred from genetic interaction with Src42A

(Muda et al., 2002)

protein phosphorylation

inferred from genetic interaction with Src42A

(Muda et al., 2002)

regulation of actin polymerization or depolymerization

inferred from mutant phenotype

salivary gland morphogenesis

inferred from mutant phenotype

(Harris and Beckendorf, 2007)

Cellular Component

CV term

References

germline ring canal

inferred from direct assay

(O'Reilly et al., 2006)

Terms Based on Predictions or Assertions ( 6 terms )

Molecular Function

CV term

(FlyBase Curators et al., 2004-)

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

protein tyrosine kinase activity

inferred from electronic annotation with InterPro:IPR008266, InterPro:IPR020635

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Simon et al., 1985)

non-traceable author statement

(Oates, 1997.11.10, Morrison et al., 2000, Swiss-Prot Project Members, 1986.7.21)

Biological Process

CV term

References

dorsal closure

traceable author statement

(Harden, 2002)

JNK cascade

traceable author statement

(Harden, 2002)

protein phosphorylation

non-traceable author statement

(Morrison et al., 2000)

Cellular Component

CV term

(Roulier et al., 1998, Guarnieri et al., 1998)

References

germline ring canal

traceable author statement

(Deng and Lin, 2001)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Protein-protein

Interacting group

Assay

References

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Src64B allele

Gene

References

Src64B^BGT-T063

RhoGAPp190

(Billuart et al., 2001)

Src64B^PI

Akap200

(Jackson and Berg, 2002)

RhoGAPp190

(Billuart et al., 2001)

Src42A

(Tateno et al., 2000)

Src64B^Scer\UAS.cPa

Csk

(Pedraza et al., 2004)

Src64B^Δ17

Btk29A

Src64B^{Δ540.hs.2sev}

Ras85D

(Kussick et al., 1993)

sina

(Kussick et al., 1993)

Src64B^Δ540.hs.sev

csw

drk

(Djagaeva et al., 2005, Chandrasekaran and Beckendorf, 2005, Djagaeva, 2005)

unspecified

Btk29A

Csk

(O'Reilly et al., 2006, Read et al., 2004)

E(Src64)2B

(Wilson et al., 2000)

pbl

(Gregory et al., 2007)

Ret

(Read et al., 2005)

Src42A

(Takahashi et al., 2005)

External Data

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0262733

Orthologs

Genome-wide drosophilid orthologs

Dana\GF24724

Dere\GG14172

Dgri\GH15800

Dmoj\GI12582

Dper\GL17941

Dpse\GA28338

Dsec\Src64B

Dsim\Src64B

Dvir\GJ15487

Dwil\GK17605

Dyak\GE20600

Curated drosophilid orthologs

Dsec\Src64B

Dsim\Src64B

y¹ v¹; P{TRiP.JF03234}attP2

Stocks & Reagents

Stocks Listed in FlyBase ( 22 )

Bloomington

8477

w^*; P{UAS-Src64B.C}2

24071

w¹¹¹⁸; Mi{ET1}Src64B^MB03494

30517

Harvard

P{XP}Src64B^d05355

PBac{PB}Src64B^c01424

PBac{PB}Src64B^c04709

PBac{RB}Src64B^e00290

PBac{WH}Src64B^f05012

PBac{WH}Src64B^f07626

Kyoto

124169

w¹¹¹⁸; P{RS3}Src64B^CB-6665-3

125958

w¹¹¹⁸; P{RS5}Src64B^5-SZ-3243

VDRC

v35252

w¹¹¹⁸ P{GD12263}v35252

More stocks available...

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 252 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

DPiM_LD30429

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

(Bayraktaroglu, 2000.4.13)

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

48973

Antibody Information

polyclonal

(Langton et al., 2009)

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Src64B CG7524

Source for merge of: Src64B MRE5

(FlyBase Genome Annotators, 2011)

Additional comments

MRE5 is part of the 3' UTR of Src64B.

(FlyBase Genome Annotators, 2011)

MRE5 corresponds to a portion of the 3' UTR of Src64B.

(FlyBase Genome Annotators, 2011)

Identified by PCR fragment; relationship to other protein tyrosine kinase genes not known.

(Oates, 1997.11.10)

Other Comments

Based on the frequency of observed ovarian defects, the following Src64B alleles can be ranked from strongest to weakest as follows: Src64B^Δ17 > Src64B^PI > Src64B^Δ19 > Src64B^KG00213.

(Bettencourt-Dias et al., 2004)

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G2/M phase cells, a whole range of mitotic abnormalities, spindle abnormalities and chromosome abnormalities are seen.

Src64B plays a role in the cytoskeletal dynamics that occur during cellularization of the blastoderm.

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Clonal analysis with Src64B^PI shows that the requirement for Src64B in mushroom body beta-lobe development is cell autonomous.

(Nicolai et al., 2003)

Src64B is essential for the proper organization of actin in ovarian ring canals.

Src64B is required for the proper growth and stability of ovarian ring canals.

(Dodson et al., 1998)

Phylogenetic analysis of the PTK family.

(Suga et al., 1997)

Abnormal eye development caused by ectopic expression of an activated form of Src64B is alleviated by dominant-negative mutations of csw or drk.

(Kussick and Cooper, 1992)

The closest relative of vertebrate c-src so far found is not Src64B, but Src42A.

(Takahashi et al., 1996)

Phosphorylation of the C-terminal tail of the Src64B protein can repress the activity of the adjacent catalytic domain of the protein.

Considered to be the Drosophila sequence homologous to mammalian c-src, based both on its origin and amino acid sequence as inferred from its nucleotide sequence.

(Lindsley and Zimm, 1992)

Src64B inferred to be a maternally acting gene whose product is required for early embryogenesis.

(Dubnau et al., 2003, Morrison et al., 2000, Quinones-Coello, 2007, Perkins et al., 2009, Aerts et al., 2010, Ni et al., 2011.7.19)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

Entrez Gene - A searchable database of RefSeq genes.

•

48973

UniProtKB/Swiss-Prot

•

P00528

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Tyrosine-protein kinase, non-receptor Src64B (IPR020703)

Protein kinase, catalytic domain (IPR000719)

SH2 motif (IPR000980)

Src homology-3 domain (IPR001452)

Tyrosine-protein kinase, active site (IPR008266)

Protein kinase-like domain (IPR011009)

Protein kinase, ATP binding site (IPR017441)

Tyrosine-protein kinase, catalytic domain (IPR020635)

Serine-threonine/tyrosine-protein kinase (IPR001245)

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0262733

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

48973

Synonyms & Secondary IDs ( 30 )

Reported As

Symbol Synonym

CG7524

c-src

(Drees et al., 1987)

C-src1

DmHD-358

(Oates, 1997.11.10, )

Dm SRC1

(Gibson and Spring, 1998)

dSrc

(Wills et al., 2002, Juang and Hoffmann, 1999, Langton et al., 2007)

dsrc

(Colson, 1998.5.26)

DSrc