FlyBase Gene Report: Dmel\Vha16-1

General Information

Symbol

Dmel\Vha16-1

Species

D. melanogaster

Name

Vacuolar H⁺ ATPase 16kD subunit 1

Annotation Symbol

CG3161

Feature Type

protein_coding_gene

FlyBase ID

FBgn0262736

Gene Model Status

Current

Stock Availability

21 publicly available

Gene Snapshot

Vacuolar H[+] ATPase 16kD subunit 1 (Vha16-1) encodes the proteolipid component of V-ATPase, which is a multi-functional channel protein. It contributes to vesicle-membrane fusion, hydrophilic molecule transport and intercellular communication. [Date last reviewed: 2019-09-26]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Vha16, MRE15, ductin, Vacuolar H⁺ ATPase 16kD subunit

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:6,625,242..6,633,090 [-]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:6625242..6633090

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

contributes_to proton-transporting ATPase activity, rotational mechanism

inferred by curator from GO:0033181

(Allan et al., 2005)

enables proton-transporting ATPase activity, rotational mechanism

inferred from biological aspect of ancestor with PANTHER:PTN000813763

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in dsRNA transport

inferred from mutant phenotype

(Saleh et al., 2006)

involved_in vacuolar acidification

inferred from mutant phenotype

(assigned by UniProt )

(Redhai et al., 2020)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proton transmembrane transport

inferred by curator from GO:0033181

(Allan et al., 2005)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in fusome

inferred from direct assay

(Lighthouse et al., 2008)

part_of plasma membrane proton-transporting V-type ATPase complex

inferred from mutant phenotype

(Allan et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in integral component of membrane

inferred from biological aspect of ancestor with PANTHER:PTN000813763

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of vacuolar proton-transporting V-type ATPase, V0 domain

inferred from sequence or structural similarity with SGD:S000000753

(Dow, 1999)

Gene Group (FlyBase)

VACUOLAR ATPASE V0 DOMAIN SUBUNITS

Protein Family (UniProt)

Belongs to the V-ATPase proteolipid subunit family. (P23380)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

VACUOLAR ATPASE V0 DOMAIN SUBUNITS -: The V0 domain of V-ATPases is the integral membrane domain responsible for the translocation of protons across the membrane. It is assembled from four to five different subunits (a, c, c'', d and e in D.mel) that are expressed as multiple isoforms in a tissue-specific manner. (Adapted from PMID:20450191 and FBrf0188241).

Protein Function (UniProtKB)

Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

(UniProt, P23380)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Vha16-1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Vha16-1-RA

FBtr0086150

NM_057453

2833

159

Vha16-1-RB

FBtr0086151

NM_165474

2798

159

Vha16-1-RC

FBtr0086152

NM_165475

2981

159

Vha16-1-RD

FBtr0086153

NM_165476

2816

159

Additional Transcript Data and Comments

Reported size (kB)

3.1 (northern blot)

(Finbow et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Vha16-1-PA

FBpp0085483

16.3

159

8.78

NP_476801

AAF57359

Vha16-1-PB

FBpp0085484

16.3

159

8.78

NP_724474

AAF57361

Vha16-1-PC

FBpp0085485

16.3

159

8.78

NP_724475

AAF57360

Vha16-1-PD

FBpp0085486

16.3

159

8.78

NP_724476

AAM68381

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

159 aa isoforms: Vha16-1-PA, Vha16-1-PB, Vha16-1-PC, Vha16-1-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

159 (aa)

(Finbow et al., 1994, Meagher et al., 1990)

Comments

External Data

Subunit Structure (UniProtKB)

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein; which is present as a hexamer that forms the proton-conducting pore).

(UniProt, P23380)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P23380

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Vha16-1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Vha16-1_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult midgut

• brush border

(Bohrmann and Bonafede, 2000)

adult gnathal ganglion | punctate

(Bohrmann and Bonafede, 2000)

indirect flight muscle

• cytoplasm & apical plasma membrane

(Bohrmann and Bonafede, 2000)

embryonic stage -- larval stage

embryonic/larval salivary gland

• apical plasma membrane

(Bohrmann and Bonafede, 2000)

embryonic stage

ventral nerve cord | punctate

(Bohrmann and Bonafede, 2000)

embryonic/larval midgut primordium

• cytoplasm | apical

(Bohrmann and Bonafede, 2000)

larval stage

neuropil of embryonic/larval brain | punctate

(Bohrmann and Bonafede, 2000)

somatic muscle | punctate

(Bohrmann and Bonafede, 2000)

oogenesis & adult stage | female

follicle cell | apical | lateral

(Lautemann and Bohrmann, 2016)

polar follicle cell

(Lautemann and Bohrmann, 2016)

centripetal follicle cell

(Lautemann and Bohrmann, 2016)

nurse cell

(Lautemann and Bohrmann, 2016)

stretch follicle cell

(Lautemann and Bohrmann, 2016)

oocyte

(Lautemann and Bohrmann, 2016)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S1 -- S7

egg chamber

Comment: 29 kD isoform

(Bohrmann and Bonafede, 2000)

oogenesis stage S10

oocyte

Comment: 29 kD isoform

(Bohrmann and Bonafede, 2000)

nurse cell

Comment: 29 kD isoform

(Bohrmann and Bonafede, 2000)

embryonic stage -- first instar larval stage

organism

Comment: predominantly 29 kD isoform

(Bohrmann and Bonafede, 2000)

third instar larval stage

embryonic/larval hypodermal muscle

Comment: predominantly 21 kD isoform

(Bohrmann and Bonafede, 2000)

embryonic/larval brain

Comment: predominantly 16 kD isoform

(Bohrmann and Bonafede, 2000)

embryonic/larval salivary gland

Comment: predominantly 16, 21 kD isoform

(Bohrmann and Bonafede, 2000)

adult stage

adult head

Comment: predominantly 21 kD isoform

(Bohrmann and Bonafede, 2000)

adult thorax

Comment: predominantly 16 kD isoform

(Bohrmann and Bonafede, 2000)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in fusome

inferred from direct assay

(Lighthouse et al., 2008)

part_of plasma membrane proton-transporting V-type ATPase complex

inferred from mutant phenotype

(Allan et al., 2005)

Expression Deduced from Reporters

Reporter: P{PTT-GA}Vha16-1^CA06708

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

germarium

• fusome

(Lighthouse et al., 2008)

Reporter: PBac{544.SVS-1}Vha16-1^CPTI100041

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

Comment: adult brain cortex high expression in these neurons (too far from the fan-shaped body to be noduli)

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Vha16-1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Vha16-1

View exonic expression by tissue for Dmel\Vha16-1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3161

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 50 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Vha16-1

Transgenic constructs containing regulatory region of Vha16-1

Deletions and Duplications ( 1 )

Partially disrupted in

Df(2R)hfl-37

(Rendina et al., 2010, Rendina et al., 2010)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Vha16-1^CA06708

lethal, with Scer\GAL4^pnr-MD237

Vha16-1^GD17431

lethal | recessive

Vha16-1^EP2372

lethal - all die before end of embryonic stage

Vha16-1^P16

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Vha16-1^GD17431

viable

viable, with Scer\GAL4^tin.CΔ4

Vha16-1^GD17431

Sterility

Allele

fertile

Other Phenotypes

Allele

decreased cell death | oogenesis stage S13, with Scer\GAL4^GR1

Vha16-1^HMS02171

neuroanatomy defective | larval stage, with Scer\GAL4^elav-C155

Vha16-1^EP2372

short lived

Vha16-1^m2

short lived | dominant

Vha16-1^EP2372

some die during embryonic stage

Vha16-1^P16

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Vha16-1^GD17431

starvation stress response defective | adult stage

Vha16-1^m2

starvation stress response defective | adult stage | dominant

Vha16-1^EP2372

Phenotype manifest in

Allele

adult midgut

anterior fascicle & synapse, with Scer\GAL4^elav-C155

Vha16-1^EP2372

dorsal appendage | oogenesis stage S14, with Scer\GAL4^GR1

Vha16-1^HMS02171

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

Vha16-1^EY00180

macrochaeta

Malpighian tubule

Vha16-1^P16

nurse cell | oogenesis stage S13, with Scer\GAL4^GR1

Vha16-1^HMS02171

nurse cell | oogenesis stage S14 | supernumerary, with Scer\GAL4^GR1

Vha16-1^HMS02171

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ATP6V0C

13 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Atp6v0c

13 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (4)

Rnor\Atp6v0c

13 of 13

Yes

Rnor\Atp5mc1

1 of 13

Rnor\Atp5mc2

1 of 13

Rnor\Atp5mc3

1 of 13

Xenopus tropicalis (Western clawed frog) (4)

Xtro\atp6v0c

13 of 12

Yes

Xtro\atp5mc1

1 of 12

Xtro\atp5mc3

1 of 12

Xtro\atp6v0b

1 of 12

Danio rerio (Zebrafish) (6)

Drer\atp6v0ca

14 of 15

Yes

Drer\atp6v0cb

14 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\vha-3

13 of 15

Yes

Cele\vha-2

7 of 15

Cele\vha-1

6 of 15

Arabidopsis thaliana (thale-cress) (5)

Atha\AVA-P2

8 of 9

Yes

Atha\AVA-P4

8 of 9

Yes

Atha\VHA-C3

7 of 9

Yes

Atha\AT2G16510

2 of 9

Yes

Atha\AVA-P1

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\VMA3

13 of 15

Yes

Scer\VMA11

7 of 15

Scer\OLI1

1 of 15

Scer\VMA16

1 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\vma3

11 of 12

Yes

Spom\vma11

5 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190HKS )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA16335

Drosophila persimilis

Dper\GL20293

Drosophila willistoni

Dwil\GK19648

Drosophila virilis

Dvir\GJ20880

Drosophila mojavensis

Dmoj\GI21278

Drosophila grimshawi

Dgri\GH22892

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150EB4 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17020365

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_00749

Mayetiola destructor

Hessian fly

Mdes\Mdes013665

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000291

Anopheles darlingi

American malaria mosquito

Adar\ADAC001959

Anopheles gambiae

Malaria mosquito

Agam\AGAP028154

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ011018

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0IFP )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA001302

Danaus plexippus

Monarch butterfly

Dple\DPOGS202679-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL014521

Apis florea

Little honeybee

Aflo\2790359300

Apis mellifera

Western honey bee

Amel\vha16

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP24751

Bombus terrestris

Buff-tailed bumblebee

Bter\15214491

Linepithema humile

Argentine ant

Lhum\LH19259

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00135c

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG010691

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_06453

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_00354

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_06820

Tribolium castaneum

Red flour beetle

Tcas\TC011025

Pediculus humanus

Human body louse

Phum\PHUM061100

Rhodnius prolixus

Kissing bug

Rpro\RPRC015179

Cimex lectularius

Bed bug

Clec\CLEC025304

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003545

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_12382

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0IJ9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR011463

Ixodes scapularis

Black-legged tick

Isca\ISCW012283

Ixodes scapularis

Black-legged tick

Isca\ISCW018346

Stegodyphus mimosarum

African social velvet spider

Smim\KFM60257.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur01g09130

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_231646

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0U1Y )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Atp16

Ciona intestinalis

Vase tunicate

Cint\LOC100175987

Gallus gallus

Domestic chicken

Ggal\ATP6V0C

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

8 of 10

7 of 10

6 of 10

6 of 10

4 of 10

3 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Vha16-1^GD4523

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Vha16-1^GD17431

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ATP6V0C; ATPase H+ transporting V0 subunit c

13 of 15

108745

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

Vha16-1 - pum

pull down, anti tag western blot

(Gerber et al., 2006)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, P23380 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262736

MIST (protein-protein) - An integrated Molecular Interaction Database

Vha16-1

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42B2-42B2

Sequence location

2R:6,625,242..6,633,090 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42B2-42B2

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

41-42

(Finbow, 1994.1.17)

42B1-42B2

(Dow, 1999)

42B1-42B2

(determined by in situ hybridisation)

(Finbow et al., 1994, Finbow et al., 1993)

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (21)

Bloomington

14814

y¹ w^67c23; P{EPgy2}EY00180

17244

w¹¹¹⁸; P{EP}Vha16-1^EP2372/CyO

65729

w¹¹¹⁸; PBac{IT.GAL4}Vha16-1^2177-G4

Kyoto

113272

w^*; P{GawB}Vha16-1^NP3427

124011

w¹¹¹⁸; P{RS3}Vha16-1^CB-6271-3

103733

w^*; P{GawB}Vha16-1^NP0734 / CyO

112967

w^*; P{GawB}Vha16-1^NP2560 / CyO

115084

w¹¹¹⁸; PBac{544.SVS-1}Vha16-1^CPTI100041

115541

w¹¹¹⁸; PBac{681.P.FSVS-1}Vha16-1^CPTI002280

VDRC

v49291

P{GD17431}v49291

v104490

P{KK109332}VIE-260B

v49290

w¹¹¹⁸; P{GD17431}v49290

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (563)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0262736

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44307

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Vha16 CG3161

(Bayraktaroglu, 2000.4.13)

Source for merge of: Vha16 BcDNA:SD02875

(FlyBase, 1996-)

Source for merge of: Vha16-1 MRE15

(FlyBase Genome Annotators, 2011)

Additional comments

MRE15 is part of the 3' UTR of Vha16-1.

(FlyBase Genome Annotators, 2011)

MRE15 corresponds to a portion of the 3' UTR of Vha16-1.

(FlyBase Genome Annotators, 2011)

Source for merge of Vha16 BcDNA:SD02875 was a shared cDNA ( date:020730 ).

(FlyBase, 1996-)

Other Comments

dsRNA made from templates generated with primers directed against Vha16 that is transfected into S2 treated with Listeria monocytogenes reveals Vha16 to be involved in Listeria monocytogenes entry and vacuolar escape.

(Cheng et al., 2005)

Vha16 has been cloned and sequenced.

(Finbow et al., 1994)

Vha16 plays a role in gap junctional communication in the follicles. Evidence suggests that the Vha16 gene product may double as a component of an ATP driven proton pump and a component of a passive, non-selective, channel.

(Finbow et al., 1993)

A Vha16 cDNA has been cloned and sequenced.

(Meagher et al., 1990)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44307

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P23380

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0262736

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Vha16-1_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3161

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44307

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG3161

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262736

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (protein-protein) - An integrated Molecular Interaction Database

Vha16-1

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

ATP6V0C

(Adamson et al., 2011)

BcDNA:SD02875

CG3161

(Neuert et al., 2017, Butzlaff et al., 2015, Meadows, 2014.12.12, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Quinones-Coello, 2007, Saleh et al., 2006, Allan et al., 2005, Laviolette et al., 2005, Wang et al., 2004, Norga et al., 2003, Labourier et al., 2002, Fulton et al., 2001)

DucC

EP2372

MRE15

(FlyBase Genome Annotators, 2011, Kageyama, 2007.2.28, Inagaki et al., 2005)

V-ATPase

(Allan et al., 2002)

Vha1

(Laviolette et al., 2005)

Vha16

(Neuert et al., 2017, Butzlaff et al., 2015, Ashwal-Fluss et al., 2014, DeSalvo et al., 2014, Carreira et al., 2011, Reis et al., 2010, Lighthouse et al., 2008, Stofanko et al., 2008, Bai et al., 2007, Buszczak et al., 2007, Langille and Clark, 2007, Quinones-Coello, 2007, Saleh et al., 2006)

Vha16-1

(Hung et al., 2020, Redhai et al., 2020, Vásquez-Procopio et al., 2020, Yalonetskaya et al., 2020, Mondragon et al., 2019, Şentürk et al., 2019, Tamirisa et al., 2018, Hsu and Drummond-Barbosa, 2017, Clandinin and Owens, 2016-, Chartier et al., 2015, Sieber and Spradling, 2015, Zhang et al., 2015, Chintapalli et al., 2013, Costa et al., 2013, Petzoldt et al., 2013, FlyBase Genome Annotators, 2011, Rendina et al., 2010, Rendina et al., 2010)

Vhac

v-ATPase

(Texada et al., 2020)

vha16

(Zoncu et al., 2011, Larsen et al., 2006, Cheng et al., 2005, Wang et al., 2004, Dow et al., 1997)

vha16-1

(Schotthöfer and Bohrmann, 2020, Lin et al., 2015, Wang et al., 2014, Chintapalli et al., 2013, Adamson et al., 2011, Allan et al., 2005, Stark et al., 2005, Dow, 1999)

Name Synonyms

Ductin

(Graham et al., 1997)

Ductin subunit C

Vacuolar H+ ATPase 16kD subunit

(Cammarato et al., 2011)

Vacuolar H+ ATPase 16kD subunit 1

Vacuolar H+ ATPase subunit 16-1

Vacuolar H+-ATPase c subunit

c subunit

(Dow et al., 1997)

ductin

(Bohrmann and Bonafede, 2000, Bohrmann and Braun, 1999, Bohrmann and Lammel, 1998, Guo et al., 1996)

ductin, vacuolar H(+)-ATPase subunit C proteolipid

(Finbow, 1994.1.17)

mRNA-like ncRNA in embryogenesis 15

Secondary FlyBase IDs

FBgn0004145
FBgn0011275
FBgn0015839
FBgn0033078
FBgn0044467
FBgn0046764
FBgn0086547

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (121)

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