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Gene Dmel\AGO1

General Information
Symbol	Dmel\AGO1	Species	D. melanogaster
Name	Argonaute-1	Annotation symbol	CG6671
Feature type	protein_coding_gene	FlyBase ID	FBgn0262739
Gene Model Status	Current	Stock availability	17 publicly available
Also Known As	dAGO1, Ago-1, l(2)04845, MRE20, Ago, l(2)k08121, l(2)k00208
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	50C9-50C17	Sequence location	2R:9,830,892..9,845,594 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Argonaute-1 is referred to in FlyBase by the symbol Dmel\AGO1 (CG6671, FBgn0262739). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: enzyme binding; protein binding; miRNA binding. There is experimental evidence for 12 unique biological process terms, many of which group under: biological regulation; single-organism developmental process; gene silencing by miRNA; gene silencing; gene expression; negative regulation of cellular biosynthetic process; multicellular organism reproduction; cellular component organization or biogenesis; heterocycle catabolic process; dsRNA fragmentation. 70 alleles are reported. The phenotypes of these alleles are annotated with: eye; macrochaeta; anterior crossvein; female germline stem cell; imaginal disc. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Argonaute/Dicer protein, PAZ; Domain of unknown function DUF1785; Ribonuclease H-like domain; Stem cell self-renewal protein Piwi. Gene sequence location is 2R:9830892..9845594.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02	Controlled Vocabulary Terms negative regulation of translation
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 50C9-50C17   Limits computationally determined from genome sequence between P{lacW}shotk04204 and P{lacW}AGO1k00208&P{lacW}Cp1k15606
Experimentally Determined Cytological Location
Cytogenetic map Notes References 50C14-50C15   (determined by in situ hybridisation) 50C17--19 (determined by in situ hybridisation) 50C12--21 (determined by in situ hybridisation)   (Spradling et al., 1999) 50C-50C     (Uemura, 1999.3.12) 50C-50C   (determined by in situ hybridisation)   (Roch et al., 1998) 50C12-50C21   (determined by in situ hybridisation)   (Preston et al., 1996) 50C14-50C15   50C17--19 50C12--21   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (Kataoka, 1999.12.2)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\AGO1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Multiple (sequential) stage-specific extensions of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated. Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.46 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) AGO1-RA FBtr0087613  NM_166020   3481   984 AGO1-RB FBtr0087614  NM_079010   5738   950 AGO1-RC FBtr0087612  NM_166021   7528   984 AGO1-RD FBtr0305592  NM_001259385   7888   984
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein AGO1-PA   FBpp0086739   109.8   984   9.36     NP_725341   AAF58314 AGO1-PB   FBpp0086740   106.2   950   9.46     NP_523734   AAF58315 AGO1-PC   FBpp0086738   109.8   984   9.36     NP_725342   AAF58313 AGO1-PD   FBpp0294043   109.8   984   9.36     NP_001246314   AFH08068
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Argonaute/Dicer protein, PAZ (IPR003100) Stem cell self-renewal protein Piwi (IPR003165) Ribonuclease H-like domain (IPR012337) Domain of unknown function DUF1785 (IPR014811)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264303   AA264831   AA390688   AA439213   AA539044   AA803333   AA941196   AA942214   AA948845   AE013599 AAF58313   AE013599 AAF58314   AE013599 AAF58315   AB035447 BAA88078   AE013599 AFH08068   AI293618   AI455659   AI456633   AI533598   AI534160   AI542292   AQ025630   AQ025680   AQ025857   AW942837   AW943353   AX482661   AY051873 AAK93297   AY069539 AAL39684   AY089537   BF492805   BF494573   BG631978   BG637394   BG637893   BG638440   BG638755   BI161835   BI161836   BI227936   BI239043   BI239659   BP541104   BP544019   BP545486   BP548628   BP550734   BP554744   BP555032   BP556576   BQ103589   BT023945   BT100007   BT125895   CA806538   CO153139   CO156206   CO178256   CO178373   CO178450   CO179497   CO186079   CO188869   CO189470   CO190377   CO192317   CO262574   CO264067   CO264552   CO264933   CO265748   CO266838   CO267186   CO268876   CO269027   CO269658   CO269991   CO270168   CO270918   CO271630   CO272564   CO273427   CO273507   CO273726   CO274453   CO274567   CO274630   CO274772   CO276043   CO277665   CO278248   CO279149   CO279420   CO279838   CO279962   CO282619   CO285019   CO285058   CO285523   CO285552   CO286015   CO290790   CO291877   CO293803   CO295063   CO295078   CO295393   CO295836   CO297264   CO297403   CO300091   CO301303   CO303185   CO303229   CO303253   CO303790   CO304122   CO304499   CO306031   CO306099   CO306843   CO307561   CO307601   CO308198   CO309482   CO309491   CO310576   CO312869   CO313945   CO315102   CO315854   CO316060   CO316135   CO318502   CO318513   CO321045   CO322907   CO324730   CO325074   CO327619   CO327793   CO328027   CO328301   CO328542   CO330687   CO330893   CO332274   CO338246   CO339388   CO340869   CO341063   CO342526   CO342640   CO343130   CZ469697   CZ472210   CZ489849   CZ489850   DN154181   DQ398917 ABD61617   DQ398918 ABD61618   DQ398919 ABD61619   DQ398920 ABD61620   DQ398921 ABD61621   DQ398922 ABD61622   DQ398923 ABD61623   DQ398924 ABD61624   EC059858   EC060110   EC060808   EC061361   EC061477   EC062113   EC063420   EC063540   EC064086   EC065638   EC066225   EC067040   EC069782   EC069913   EC071964   EC072326   EC074358   EC075872   EC077282   EC077387   EC077542   EC080195   EC082630   EC082779   EC084096   EC084330   EC085556   EC088520   EC089304   EC089735   EC089738   EC090368   EC197199   EC199851   EC200874   EC202101   EC202283   EC202799   EC203441   EC203940   EC207874   EC207919   EC209236   EC209358   EC209388   EC209991   EC210106   EC210473   EC210576   EC210629   EC211035   EC212288   EC214102   EC214630   EC217614   EC217619   EC218143   EC218381   EC220972   EC221027   EC222723   EC223193   EC224719   EC225499   EC225802   EC225940   EC226447   EC226803   EC227928   EC229932   EC230225   EC230937   EC234980   EC236390   EC236767   EC241222   EC241649   EC243268   EC245998   EC246719   EC248855   EC248904   EC249236   EC249744   EC252000   EC252379   EC255387   EC256077   EC256620   EC259190   EC259336   EC261640   EC263969   EC264511   ES695713   EV583746   EV587663   EV587865   EV587930   EV599498
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KY08 Q32KD4 Q960T1 Q8T059 Q27IR0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Grimaud et al., 2006, Lee et al., 2009, Reich et al., 2009, Gehrke et al., 2010, Azzam et al., 2012) nucleus inferred from direct assay (Grimaud et al., 2006) perinuclear region of cytoplasm inferred from direct assay (Lee et al., 2009) ribonucleoprotein complex inferred from direct assay (Liu et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3    141 central nervous system, pupae P8    137 head, virgin 1-day female    64 head, virgin 4-day female    60 head, virgin 20-day female    31 head, mated 1-day female    61 head, mated 4-day female    50 head, mated 20-day female    34 head, mated 1-day male    82 head, mated 4-day male    88 head, mated 20-day male    66 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    25 digestive system, 1-day adult    17 digestive system, 4-day adult    31 digestive system, 20-day adult    22 fat body, larvae L3 wandering    16 fat body, white prepupae    31 fat body, pupae P8    34 carcass, larvae L3 wandering    63 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    24 ovary, virgin 4-day female    75 ovary, mated 4-day female    79 testis, mated 4-day male    4 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (58) central nervous system, larvae L3  (141) central nervous system, pupae P8  (137) head, virgin 1-day female  (64) head, virgin 4-day female  (60) head, virgin 20-day female  (31) head, mated 1-day female  (61) head, mated 4-day female  (50) head, mated 20-day female  (34) head, mated 1-day male  (82) head, mated 4-day male  (88) head, mated 20-day male  (66) salivary gland, larvae L3 wandering    13 salivary gland, white prepupae  (57) digestive system, larvae L3 wandering    25 digestive system, 1-day adult    17 digestive system, 4-day adult  (31) digestive system, 20-day adult    22 fat body, larvae L3 wandering    16 fat body, white prepupae  (31) fat body, pupae P8  (34) carcass, larvae L3 wandering  (63) carcass, 1-day adult  (29) carcass, 4-day adult  (31) carcass, 20-day adult    24 ovary, virgin 4-day female  (75) ovary, mated 4-day female  (79) testis, mated 4-day male    4 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3  (141) central nervous system, pupae P8  (137) head, virgin 1-day female    64 head, virgin 4-day female    60 head, virgin 20-day female    31 head, mated 1-day female    61 head, mated 4-day female    50 head, mated 20-day female    34 head, mated 1-day male    82 head, mated 4-day male    88 head, mated 20-day male    66 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    25 digestive system, 1-day adult    17 digestive system, 4-day adult    31 digestive system, 20-day adult    22 fat body, larvae L3 wandering    16 fat body, white prepupae    31 fat body, pupae P8    34 carcass, larvae L3 wandering    63 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    24 ovary, virgin 4-day female    75 ovary, mated 4-day female    79 testis, mated 4-day male    4 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3    141 central nervous system, pupae P8    137 head, virgin 1-day female    64 head, virgin 4-day female    60 head, virgin 20-day female    31 head, mated 1-day female    61 head, mated 4-day female    50 head, mated 20-day female    34 head, mated 1-day male    82 head, mated 4-day male    88 head, mated 20-day male    66 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    25 digestive system, 1-day adult    17 digestive system, 4-day adult    31 digestive system, 20-day adult    22 fat body, larvae L3 wandering    16 fat body, white prepupae    31 fat body, pupae P8    34 carcass, larvae L3 wandering    63 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    24 ovary, virgin 4-day female    75 ovary, mated 4-day female    79 testis, mated 4-day male    4 accessory gland, mated 4-day male    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3    141 central nervous system, pupae P8    137 head, virgin 1-day female    64 head, virgin 4-day female    60 head, virgin 20-day female    31 head, mated 1-day female    61 head, mated 4-day female    50 head, mated 20-day female    34 head, mated 1-day male    82 head, mated 4-day male    88 head, mated 20-day male    66 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    25 digestive system, 1-day adult    17 digestive system, 4-day adult    31 digestive system, 20-day adult    22 fat body, larvae L3 wandering    16 fat body, white prepupae    31 fat body, pupae P8    34 carcass, larvae L3 wandering    63 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    24 ovary, virgin 4-day female    75 ovary, mated 4-day female    79 testis, mated 4-day male    4 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (58) central nervous system, larvae L3  (141) central nervous system, pupae P8  (137) head, virgin 1-day female  (64) head, virgin 4-day female  (60) head, virgin 20-day female  31 head, mated 1-day female  (61) head, mated 4-day female  (50) head, mated 20-day female  (34) head, mated 1-day male  (82) head, mated 4-day male  (88) head, mated 20-day male  (66) salivary gland, larvae L3 wandering    13 salivary gland, white prepupae  (57) digestive system, larvae L3 wandering    25 digestive system, 1-day adult    17 digestive system, 4-day adult  31 digestive system, 20-day adult    22 fat body, larvae L3 wandering    16 fat body, white prepupae  31 fat body, pupae P8  (34) carcass, larvae L3 wandering  (63) carcass, 1-day adult  29 carcass, 4-day adult  31 carcass, 20-day adult    24 ovary, virgin 4-day female  (75) ovary, mated 4-day female  (79) testis, mated 4-day male    4 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3  141 central nervous system, pupae P8  137 head, virgin 1-day female    64 head, virgin 4-day female    60 head, virgin 20-day female    31 head, mated 1-day female    61 head, mated 4-day female    50 head, mated 20-day female    34 head, mated 1-day male    82 head, mated 4-day male    88 head, mated 20-day male    66 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    25 digestive system, 1-day adult    17 digestive system, 4-day adult    31 digestive system, 20-day adult    22 fat body, larvae L3 wandering    16 fat body, white prepupae    31 fat body, pupae P8    34 carcass, larvae L3 wandering    63 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    24 ovary, virgin 4-day female    75 ovary, mated 4-day female    79 testis, mated 4-day male    4 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    58 central nervous system, larvae L3    141 central nervous system, pupae P8    137 head, virgin 1-day female    64 head, virgin 4-day female    60 head, virgin 20-day female    31 head, mated 1-day female    61 head, mated 4-day female    50 head, mated 20-day female    34 head, mated 1-day male    82 head, mated 4-day male    88 head, mated 20-day male    66 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    57 digestive system, larvae L3 wandering    25 digestive system, 1-day adult    17 digestive system, 4-day adult    31 digestive system, 20-day adult    22 fat body, larvae L3 wandering    16 fat body, white prepupae    31 fat body, pupae P8    34 carcass, larvae L3 wandering    63 carcass, 1-day adult    29 carcass, 4-day adult    31 carcass, 20-day adult    24 ovary, virgin 4-day female    75 ovary, mated 4-day female    79 testis, mated 4-day male    4 accessory gland, mated 4-day male    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    66 embryo 04-06hr    57 embryo 06-08hr    58 embryo 08-10hr    57 embryo 10-12hr    40 embryo 12-14hr    55 embryo 14-16hr    50 embryo 16-18hr    47 embryo 18-20hr    33 embryo 20-22hr    38 embryo 22-24hr    44 larva L1    25 larva L2    16 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    35 white prepupae new    40 white prepupae 12hr    33 white prepupae 24hr    39 pupae 2d postWPP    39 pupae 3d postWPP    24 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    30 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (46) embryo 02-04hr  (66) embryo 04-06hr  (57) embryo 06-08hr  (58) embryo 08-10hr  (57) embryo 10-12hr  (40) embryo 12-14hr  (55) embryo 14-16hr  (50) embryo 16-18hr  (47) embryo 18-20hr  (33) embryo 20-22hr  (38) embryo 22-24hr  (44) larva L1    25 larva L2    16 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9  (35) white prepupae new  (40) white prepupae 12hr  (33) white prepupae 24hr  (39) pupae 2d postWPP  (39) pupae 3d postWPP    24 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day  (30) adult female 30day  (29) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    66 embryo 04-06hr    57 embryo 06-08hr    58 embryo 08-10hr    57 embryo 10-12hr    40 embryo 12-14hr    55 embryo 14-16hr    50 embryo 16-18hr    47 embryo 18-20hr    33 embryo 20-22hr    38 embryo 22-24hr    44 larva L1    25 larva L2    16 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    35 white prepupae new    40 white prepupae 12hr    33 white prepupae 24hr    39 pupae 2d postWPP    39 pupae 3d postWPP    24 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    30 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    66 embryo 04-06hr    57 embryo 06-08hr    58 embryo 08-10hr    57 embryo 10-12hr    40 embryo 12-14hr    55 embryo 14-16hr    50 embryo 16-18hr    47 embryo 18-20hr    33 embryo 20-22hr    38 embryo 22-24hr    44 larva L1    25 larva L2    16 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    35 white prepupae new    40 white prepupae 12hr    33 white prepupae 24hr    39 pupae 2d postWPP    39 pupae 3d postWPP    24 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    30 adult female 30day    29 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    66 embryo 04-06hr    57 embryo 06-08hr    58 embryo 08-10hr    57 embryo 10-12hr    40 embryo 12-14hr    55 embryo 14-16hr    50 embryo 16-18hr    47 embryo 18-20hr    33 embryo 20-22hr    38 embryo 22-24hr    44 larva L1    25 larva L2    16 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    35 white prepupae new    40 white prepupae 12hr    33 white prepupae 24hr    39 pupae 2d postWPP    39 pupae 3d postWPP    24 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    30 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (46) embryo 02-04hr  (66) embryo 04-06hr  (57) embryo 06-08hr  (58) embryo 08-10hr  (57) embryo 10-12hr  (40) embryo 12-14hr  (55) embryo 14-16hr  (50) embryo 16-18hr  (47) embryo 18-20hr  (33) embryo 20-22hr  (38) embryo 22-24hr  (44) larva L1    25 larva L2    16 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9  (35) white prepupae new  (40) white prepupae 12hr  (33) white prepupae 24hr  (39) pupae 2d postWPP  (39) pupae 3d postWPP    24 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day  30 adult female 30day  29 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    66 embryo 04-06hr    57 embryo 06-08hr    58 embryo 08-10hr    57 embryo 10-12hr    40 embryo 12-14hr    55 embryo 14-16hr    50 embryo 16-18hr    47 embryo 18-20hr    33 embryo 20-22hr    38 embryo 22-24hr    44 larva L1    25 larva L2    16 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    35 white prepupae new    40 white prepupae 12hr    33 white prepupae 24hr    39 pupae 2d postWPP    39 pupae 3d postWPP    24 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    30 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    66 embryo 04-06hr    57 embryo 06-08hr    58 embryo 08-10hr    57 embryo 10-12hr    40 embryo 12-14hr    55 embryo 14-16hr    50 embryo 16-18hr    47 embryo 18-20hr    33 embryo 20-22hr    38 embryo 22-24hr    44 larva L1    25 larva L2    16 larva L3 12hr old    11 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    25 larva L3 puffstage 7-9    35 white prepupae new    40 white prepupae 12hr    33 white prepupae 24hr    39 pupae 2d postWPP    39 pupae 3d postWPP    24 pupae 4d postWPP    14 adult male 01day    18 adult male 05day    16 adult male 30day    15 adult female 01day    25 adult female 05day    30 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    44 embryonic 1182-4H    47 embryonic GM2    82 embryonic Kc167    58 embryonic S1    36 embryonic S3    71 leg disc CME L1    43 wing disc CME-W2    49 wing disc ML-DmD8    51 wing disc ML-DmD9    32 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    21 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    67 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    54 tumorous blood cells mbn2    64 ovary fGS/OSS    98 ovary OSC    81 ovary OSS    86 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (47) Schneider line 2 Sg4  (44) embryonic 1182-4H  (47) embryonic GM2  (82) embryonic Kc167  (58) embryonic S1  (36) embryonic S3  (71) leg disc CME L1  (43) wing disc CME-W2  (49) wing disc ML-DmD8  (51) wing disc ML-DmD9  (32) wing disc ML-DmD16-c3  (52) wing disc ML-DmD21    21 wing disc ML-DmD32  (48) haltere disc ML-DmD17-c3  (67) eye-antennal disc ML-DmD11  (28) antennal disc ML-DmD20-c5  26 mixed discs ML-DmD4-c1  (38) CNS ML-DmBG1-c1  (39) CNS ML-DmBG2-c2  (54) tumorous blood cells mbn2  (64) ovary fGS/OSS  (98) ovary OSC  (81) ovary OSS  (86) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    44 embryonic 1182-4H    47 embryonic GM2    82 embryonic Kc167    58 embryonic S1    36 embryonic S3    71 leg disc CME L1    43 wing disc CME-W2    49 wing disc ML-DmD8    51 wing disc ML-DmD9    32 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    21 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    67 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    54 tumorous blood cells mbn2    64 ovary fGS/OSS    98 ovary OSC    81 ovary OSS    86 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    44 embryonic 1182-4H    47 embryonic GM2    82 embryonic Kc167    58 embryonic S1    36 embryonic S3    71 leg disc CME L1    43 wing disc CME-W2    49 wing disc ML-DmD8    51 wing disc ML-DmD9    32 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    21 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    67 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    54 tumorous blood cells mbn2    64 ovary fGS/OSS    98 ovary OSC    81 ovary OSS    86 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    44 embryonic 1182-4H    47 embryonic GM2    82 embryonic Kc167    58 embryonic S1    36 embryonic S3    71 leg disc CME L1    43 wing disc CME-W2    49 wing disc ML-DmD8    51 wing disc ML-DmD9    32 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    21 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    67 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    54 tumorous blood cells mbn2    64 ovary fGS/OSS    98 ovary OSC    81 ovary OSS    86 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (47) Schneider line 2 Sg4  (44) embryonic 1182-4H  (47) embryonic GM2  (82) embryonic Kc167  (58) embryonic S1  (36) embryonic S3  (71) leg disc CME L1  (43) wing disc CME-W2  (49) wing disc ML-DmD8  (51) wing disc ML-DmD9  32 wing disc ML-DmD16-c3  (52) wing disc ML-DmD21    21 wing disc ML-DmD32  (48) haltere disc ML-DmD17-c3  (67) eye-antennal disc ML-DmD11  28 antennal disc ML-DmD20-c5  26 mixed discs ML-DmD4-c1  (38) CNS ML-DmBG1-c1  (39) CNS ML-DmBG2-c2  (54) tumorous blood cells mbn2  (64) ovary fGS/OSS  (98) ovary OSC  (81) ovary OSS  (86) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    44 embryonic 1182-4H    47 embryonic GM2    82 embryonic Kc167    58 embryonic S1    36 embryonic S3    71 leg disc CME L1    43 wing disc CME-W2    49 wing disc ML-DmD8    51 wing disc ML-DmD9    32 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    21 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    67 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    54 tumorous blood cells mbn2    64 ovary fGS/OSS    98 ovary OSC    81 ovary OSS    86 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    47 Schneider line 2 Sg4    44 embryonic 1182-4H    47 embryonic GM2    82 embryonic Kc167    58 embryonic S1    36 embryonic S3    71 leg disc CME L1    43 wing disc CME-W2    49 wing disc ML-DmD8    51 wing disc ML-DmD9    32 wing disc ML-DmD16-c3    52 wing disc ML-DmD21    21 wing disc ML-DmD32    48 haltere disc ML-DmD17-c3    67 eye-antennal disc ML-DmD11    28 antennal disc ML-DmD20-c5    26 mixed discs ML-DmD4-c1    38 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    54 tumorous blood cells mbn2    64 ovary fGS/OSS    98 ovary OSC    81 ovary OSS    86 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    19 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    72 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    140 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    69 Ethanol 2.5% 3 hrs, larvae L3    35 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    19 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult  (32) Cadmium 100 mM 48 hrs, 4-day adult  (72) Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult  (140) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult  (69) Ethanol 2.5% 3 hrs, larvae L3  (35) Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (68) Caffeine 25 mg/ml 48 hrs, 4-day adult  (34) Paraquat 5 mM 48 hrs, 4-day adult  (48) Paraquat 10 mM 48 hrs, 4-day adult  (31) Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    19 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    72 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult  (140) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    69 Ethanol 2.5% 3 hrs, larvae L3    35 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    19 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    72 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    140 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    69 Ethanol 2.5% 3 hrs, larvae L3    35 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    19 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    72 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    140 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    69 Ethanol 2.5% 3 hrs, larvae L3    35 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    19 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult  32 Cadmium 100 mM 48 hrs, 4-day adult  (72) Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult  (140) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult  (69) Ethanol 2.5% 3 hrs, larvae L3  (35) Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (68) Caffeine 25 mg/ml 48 hrs, 4-day adult  (34) Paraquat 5 mM 48 hrs, 4-day adult  (48) Paraquat 10 mM 48 hrs, 4-day adult  31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    19 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    72 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult  140 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    69 Ethanol 2.5% 3 hrs, larvae L3    35 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    19 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    72 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    140 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    69 Ethanol 2.5% 3 hrs, larvae L3    35 Ethanol 5% 3 hrs, larvae L3    16 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    19 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    68 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_05 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage (with AGO1D743N) AGO1Q127X lethal | embryonic stage (with AGO1E808K) AGO1Q127X lethal | embryonic stage (with AGO1Q127X) AGO1D743N AGO1E808K AGO1R839X AGO1R937C lethal | embryonic stage (with AGO1R839X) AGO1Q127X lethal | embryonic stage (with AGO1R937C) AGO1Q127X lethal | embryonic stage | recessive AGO1k08121 lethal | larval stage (with AGO1D743N) AGO1Q127X lethal | larval stage (with AGO1E808K) AGO1Q127X lethal | larval stage (with AGO1Q127X) AGO1D743N AGO1E808K AGO1R937C lethal | larval stage (with AGO1R937C) AGO1Q127X lethal | larval stage | recessive AGO1k00208 lethal | pupal stage (with AGO1D743N) AGO1Q127X lethal | pupal stage (with AGO1Q127X) AGO1D743N lethal | recessive AGO104845 AGO1D743N AGO1E808K AGO1J04 AGO1k00208 AGO1k08121 AGO1k16601 AGO1Q127X AGO1R839X AGO1R937C AGO1T908M AGO1W894X partially lethal - majority die AGO104845 viable AGO1CA06914 Other Phenotypes Allele increased cell death | germline clone AGO1k08121 visible, with Scer\GAL4en-e16E AGO1Scer\UAS.cKa visible, with Scer\GAL4GMR.PF AGO1Scer\UAS.cZa visible, with Scer\GAL4ptc-559.1 AGO1Scer\UAS.cKa AGO1ΔC.Scer\UAS AGO1ΔN.Scer\UAS visible, with Scer\GAL4sca-537.4 AGO1Scer\UAS.cKa visible, with Scer\GAL4vg.PM AGO1Scer\UAS.cKa visible | somatic clone AGO1D743N AGO1E808K AGO1Q127X AGO1R839X AGO1R937C AGO1T908M wild-type AGO1hs.PK Phenotype manifest in Allele anterior crossvein, with Scer\GAL4ptc-559.1 AGO1ΔN.Scer\UAS AGO1Scer\UAS.cKa AGO1ΔC.Scer\UAS chordotonal organ & neuron | germ-line clone AGO1k08121 commissure | germline clone AGO1k08121 denticle belt | germline clone (with Df(2R)50C-45) AGO1k08121 denticle row 1 | germline clone (with Df(2R)50C-45) AGO1k08121 embryonic/larval glial cell | germline clone AGO1k08121 eye, with Scer\GAL4GMR.PF AGO1Scer\UAS.cZa eye | somatic clone AGO1Q127X AGO1D743N AGO1E808K AGO1R839X AGO1T908M AGO1R937C female germline stem cell AGO1k08121 female germline stem cell (with AGO114) AGO1k08121 female germline stem cell (with AGO1EMS) AGO1k08121 female germline stem cell (with AGO1k08121) AGO1EMS AGO114 female germline stem cell | supernumerary | heat sensitive AGO1hs.PK imaginal disc AGO1k00208 longitudinal connective | germline clone AGO1k08121 macrochaeta, with Scer\GAL4en-e16E AGO1Scer\UAS.cKa oocyte | germline clone AGO1EMS AGO114 AGO1k08121 presumptive embryonic/larval peripheral nervous system | germline clone AGO1k08121 wing | posterior compartment, with Scer\GAL4sca-537.4 AGO1Scer\UAS.cKa
Classical Alleles ( 46 )
For All Classical Alleles Show
Allele of AGO1 Class Mutagen Stocks Known lesion AGO1k00208 P-element activity 2 Yes AGO1k08121 loss of function allele P-element activity 2 Yes AGO104845 P-element activity 1 Yes AGO1c05033 piggyBac transposase 1 -- AGO1d01402 P-element activity 1 -- AGO1e01877 1 -- AGO1EY21521 P-element activity 1 -- AGO1f07079 piggyBac transposase 1 -- AGO1G2846 P-element activity 1 -- AGO1k16601 P-element activity 1 -- AGO1NP2077 1 -- AGO1NP2416 1 -- AGO1NP3498 1 -- AGO1Q127X amorphic allele - molecular evidence ethyl methanesulfonate 0 Yes AGO1R839X amorphic allele - molecular evidence ethyl methanesulfonate 0 Yes AGO1W894X amorphic allele - molecular evidence ethyl methanesulfonate 0 Yes AGO110 P-element activity 0 Yes AGO111 P-element activity 0 Yes AGO112 P-element activity 0 Yes AGO113 P-element activity 0 Yes AGO114 P-element activity 0 Yes AGO115 P-element activity 0 Yes AGO11 P-element activity 0 Yes AGO12 P-element activity 0 Yes AGO13 P-element activity 0 Yes AGO145 loss of function allele Delta2-3 0 Yes AGO14 P-element activity 0 Yes AGO15-HA-2971 0 -- AGO150C-102 0 -- AGO150C-68 0 -- AGO150C-81 0 -- AGO150C-85 0 -- AGO15 P-element activity 0 Yes AGO16 P-element activity 0 Yes AGO172 loss of function allele Delta2-3 0 Yes AGO17 P-element activity 0 Yes AGO18 P-element activity 0 Yes AGO19 P-element activity 0 Yes AGO1CA06914 P-element activity 0 Yes AGO1D743N ethyl methanesulfonate 0 Yes AGO1E808K ethyl methanesulfonate 0 Yes AGO1EMS ethyl methanesulfonate 0 Yes AGO1J04 ethyl methanesulfonate 0 -- AGO1R937C ethyl methanesulfonate 0 Yes AGO1SH1307 0 -- AGO1T908M ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 24 )
For All Alleles Carried on Transgenic Constructs Show
Allele of AGO1 Class Mutagen Stocks Known lesion AGO1HM04006 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AGO1HMS00610 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AGO1Act5C.T:λ\N in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes AGO1dsRNA.cCa in vitro construct - RNAi 0 Yes AGO1dsRNA.cDa in vitro construct - RNAi 0 Yes AGO1dsRNA.cFa in vitro construct - RNAi 0 Yes AGO1dsRNA.cJa in vitro construct - RNAi 0 Yes AGO1dsRNA.cJb in vitro construct - RNAi 0 -- AGO1dsRNA.cOa in vitro construct - RNAi 0 Yes AGO1dsRNA.cOb in vitro construct - RNAi 0 Yes AGO1GD11997 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes AGO1hs.PK in vitro construct - regulatory fusion 0 Yes AGO1hs.PW in vitro construct - regulatory fusion 0 -- AGO1Hsp83.T:Zzzz\FLAG,T:Zzzz\StrepII,T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes AGO1K657A.K694A.Hsp83.T:Zzzz\FLAG,T:Zzzz\StrepII,T:Zzzz\His6 in vitro construct - amino acid replacement 0 Yes AGO1MtnA.T:Uuuu\T7 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes AGO1MtnA.T:Zzzz\TAP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes AGO1Scer\UAS.cDa in vitro construct - regulatory fusion 0 Yes AGO1Scer\UAS.cKa in vitro construct - regulatory fusion 0 Yes AGO1Scer\UAS.cZa in vitro construct - regulatory fusion 0 Yes AGO1ΔC.hs in vitro construct - deletion 0 Yes AGO1ΔC.Scer\UAS in vitro construct - deletion 0 Yes AGO1ΔN.hs in vitro construct - deletion 0 Yes AGO1ΔN.Scer\UAS in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(2R)50C-16 (Preston et al., 1996) Df(2R)50C-21 (Preston et al., 1996) Df(2R)50C-36 (Preston et al., 1996) Df(2R)50C-38 (Preston et al., 1996) Df(2R)50C-40 (Preston et al., 1996) Df(2R)50C-43 (Preston et al., 1996) Df(2R)50C-49 (Preston et al., 1996) Df(2R)50C-68 (Preston et al., 1996) Df(2R)50C-69 (Preston et al., 1996) Df(2R)50C-71 (Preston et al., 1996) Df(2R)50C-81 (Preston et al., 1996) Df(2R)50C-85 (Preston et al., 1996) Df(2R)50C-89 (Preston et al., 1996) Df(2R)50C-101 (Preston et al., 1996) Df(2R)50C-111 (Preston et al., 1996) Df(2R)50C-116 (Preston et al., 1996) Df(2R)50C-128 (Preston et al., 1996) Df(2R)50C-129 (Preston et al., 1996) Df(2R)50C-131 (Preston et al., 1996) Df(2R)50C-132 (Preston et al., 1996)
Disrupted in	Df(2R)50C-38 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)50C-45 (BDGP Project Members, 1994-1999, Preston et al., 1996, Kataoka et al., 2001) Df(2R)50C-101 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)50C-102 (BDGP Project Members, 1994-1999, Preston et al., 1996) Df(2R)50C-107 (Preston et al., 1996) Df(2R)50C-133 (Preston et al., 1996) Df(2R)CX1 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2R)Exel7129 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11997} NA P{TRiP.HM04006} NA P{TRiP.HMS00610} NA P{UAS-AGO1.D} NA P{UAS-AGO1.K} NA P{UAS-AGO1.Z} NA P{UAS-AGO1.ΔC} NA P{UAS-AGO1.ΔN} NA heat-shock construct P{hs-AGO1.K} NA P{hs-AGO1.W} NA P{hs-AGO1.ΔC} NA P{hs-AGO1.ΔN} NA P{Hsp83-AGO1.FSH} NA P{Hsp83-AGO1.K657A.K694A.FSH} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}AGO172 No P{lacW}AGO1k00208 No P{lacW}AGO1k08121 No P{lacW}AGO1k16601 No P{lacW}AGO1SH1307 No P{lacW}l(2)SH1067SH1067 No P{PZ}AGO104845 No miscellaneous insertions P{?'lacW}AGO145 NA P{PTT-GA}AGO1CA06914 NA P{RS5}AGO15-HA-2971 NA PBac{PB}AGO1c05033 NA PBac{RB}AGO1e01877 NA PBac{WH}AGO1f07079 NA insertion of enhancer trap binary system P{GawB}AGO1NP2077 No P{GawB}AGO1NP2416 No P{GawB}AGO1NP3498 No insertion of mobile activating element P{EP}AGO1G2846 NA P{EPgy2}AGO1EY21521 NA P{GSV1}C478C478 NA P{GSV1}GSd453GSd453 NA P{Mae-UAS.6.11}B1517B1517 NA P{XP}AGO1d01402 NA
Gene Ontology: Function, Process & Cellular Component ( 24 unique terms )
Terms Based on Experimental Evidence ( 19 terms )
Molecular Function
CV term Evidence References
enzyme binding inferred from physical interaction with Dcr-1 (Okamura et al., 2004) miRNA binding inferred from direct assay (Vagin et al., 2006) protein binding inferred from physical interaction with AGO2 (Meyer et al., 2006) inferred from physical interaction with UniProtKB:Q8SY33 (assigned by UniProtKB) (Eulalio et al., 2008, Chekulaeva et al., 2010, Eulalio et al., 2009)
Biological Process
CV term Evidence References
chromatin silencing inferred from mutant phenotype (Grimaud et al., 2006) female germ-line stem cell maintenance inferred from mutant phenotype (Yang et al., 2007) gene silencing by miRNA inferred from mutant phenotype (Rehwinkel et al., 2005) germarium-derived female germ-line cyst formation inferred from mutant phenotype (Azzam et al., 2012) germarium-derived oocyte fate determination inferred from mutant phenotype (Azzam et al., 2012) mRNA catabolic process inferred from genetic interaction with gw (Behm-Ansmant et al., 2006) inferred from mutant phenotype (Behm-Ansmant et al., 2006) mRNA cleavage involved in gene silencing by miRNA inferred from direct assay (Miyoshi et al., 2005) inferred from mutant phenotype (Okamura et al., 2004) negative regulation of translation inferred from mutant phenotype (Pinder and Smibert, 2013) production of miRNAs involved in gene silencing by miRNA inferred from mutant phenotype (Okamura et al., 2004) production of siRNA involved in RNA interference inferred from mutant phenotype (Williams and Rubin, 2002) segment polarity determination inferred from genetic interaction with AGO2 (Meyer et al., 2006) inferred from genetic interaction with Dcr-1 (Meyer et al., 2006) targeting of mRNA for destruction involved in RNA interference inferred from mutant phenotype (Williams and Rubin, 2002)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Grimaud et al., 2006, Lee et al., 2009, Reich et al., 2009, Gehrke et al., 2010, Azzam et al., 2012) nucleus inferred from direct assay (Grimaud et al., 2006) perinuclear region of cytoplasm inferred from direct assay (Lee et al., 2009) ribonucleoprotein complex inferred from direct assay (Liu et al., 2011)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
translation initiation factor activity inferred from sequence or structural similarity with UniProtKB:Q9UL18 (FlyBase, 1992-)
Biological Process
CV term Evidence References
gene silencing by miRNA non-traceable author statement (Skipper, 2004) RNA interference traceable author statement (Cerutti, 2003) synapse assembly traceable author statement (Murchison and Hannon, 2004)
Cellular Component
CV term Evidence References
eukaryotic translation initiation factor 2 complex inferred from sequence or structural similarity with UniProtKB:Q9UL18 (FlyBase, 1992-) RNA-induced silencing complex inferred from sequence or structural similarity with UniProtKB:O48771 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
AGO1 - Atx2 experimental knowledge based (Guruharsha et al., 2011) AGO1 - CG10077 experimental knowledge based (Guruharsha et al., 2011) AGO1 - CG11505 experimental knowledge based (Guruharsha et al., 2011) AGO1 - CG34159 experimental knowledge based (Guruharsha et al., 2011) AGO1 - CG7185 experimental knowledge based (Guruharsha et al., 2011) AGO1 - CG7878 experimental knowledge based (Guruharsha et al., 2011) AGO1 - Lrrk anti bait coimmunoprecipitation, western blot (Gehrke et al., 2010) AGO1 - Nbr anti tag coimmunoprecipitation, anti tag western blot (Liu et al., 2011) AGO1 - Psi experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions AGO1 allele Gene References AGO1Scer\UAS.cKa shg (Kataoka et al., 2001) AGO1ΔC.Scer\UAS shg (Kataoka et al., 2001) AGO1ΔN.Scer\UAS shg (Kataoka et al., 2001) unspecified AGO2 (Meyer et al., 2006) shg (Kataoka et al., 1999)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262739
Orthologs
OrthoDB Orthologs (64) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6QZ858 Dipteran inclusive ortholog search EOG6CNR04 Insect inclusive ortholog search EOG6H716H Arthropod inclusive ortholog search EOG6NVX17 Metazoa inclusive ortholog search EOG6R873P
Orthologs in Drosophila Species (EOG6QZ858)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\AGO1     Drosophila simulans   Dsim\AGO1 Y   Drosophila sechellia   Dsec\GM23254 Y   Drosophila erecta   Dere\GG22457 Y   Drosophila yakuba   Dyak\GE13329 Y   Drosophila ananassae   Dana\GF11153 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19767 Y   Drosophila persimilis   Dper\GL17077 Y   Drosophila willistoni   Dwil\GK17851 Y   Drosophila virilis   Dvir\GJ21530 Y   Drosophila mojavensis   Dmoj\GI18445 Y   Drosophila grimshawi   Dgri\GH22955 Y
Orthologs in non-Drosophila Dipterans (EOG6CNR04)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL015246   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012410   Anopheles gambiae Malaria mosquito  Agam\AGAP011717   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006138
Orthologs in non-Dipteran Insects (EOG6H716H)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB48208   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG026090   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG026082   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH26792   Atta cephalotes Leafcutter ant  Acep\ACEP21829   Atta cephalotes Leafcutter ant  Acep\ACEP23493   Camponotus floridanus Florida carpenter ant  Cflo\CFLO20819   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17607   Linepithema humile Argentine ant  Lhum\LH15418   Pogonomyrmex barbatus Red harvester ant  Pbar\PB23731   Solenopsis invicta Red fire ant  Sinv\SINV25027   Acyrthosiphon pisum Pea aphid  Apim\ACYPI007150   Bombyx mori Silkmoth  Bmor\Ago1   Pediculus humanus Human body louse  Phum\PHUM617260   Tribolium castaneum Red flour beetle  Tcas\Ago1
Orthologs in non-Insect Arthropods (EOG6NVX17)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_305022   Ixodes scapularis Deer tick  Isca\ISCW013378
Orthologs in non-Arthropod Metazoa (EOG6R873P)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\alg-2   Caenorhabditis elegans Nematode  Cele\alg-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023100gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020628gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019390gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019389gn   Danio rerio Zebrafish  Drer\eif2c3   Danio rerio Zebrafish  Drer\ENSDARG00000086514   Danio rerio Zebrafish  Drer\CU929240.1   Danio rerio Zebrafish  Drer\ENSDARG00000092644   Danio rerio Zebrafish  Drer\ENSDARG00000059888   Xenopus tropicalis Western clawed frog  Xtro\eif2c3   Xenopus tropicalis Western clawed frog  Xtro\eif2c2   Xenopus tropicalis Western clawed frog  Xtro\eif2c1   Xenopus tropicalis Western clawed frog  Xtro\eif2c4   Gallus gallus Domestic chicken  Ggal\EIF2C2   Gallus gallus Domestic chicken  Ggal\EIF2C3   Gallus gallus Domestic chicken  Ggal\EIF2C1   Gallus gallus Domestic chicken  Ggal\EIF2C4   Mus musculus House mouse  Mmus\Eif2c4   Mus musculus House mouse  Mmus\Eif2c1   Mus musculus House mouse  Mmus\Eif2c2   Mus musculus House mouse  Mmus\Eif2c3   Rattus norvegicus Norway rat  Rnor\Eif2c3   Rattus norvegicus Norway rat  Rnor\Eif2c1   Rattus norvegicus Norway rat  Rnor\Eif2c2   Rattus norvegicus Norway rat  Rnor\Eif2c4   Homo sapiens Human  Hsap\EIF2C4   Homo sapiens Human  Hsap\EIF2C1   Homo sapiens Human  Hsap\EIF2C3   Homo sapiens Human  Hsap\EIF2C2
Human Orthologs (4)
Gene OMIM HGNC Hsap\EIF2C4     Hsap\EIF2C1     Hsap\EIF2C3     Hsap\EIF2C2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\AGO1 Drosophila sechellia Dsec\GM23254 Drosophila erecta Dere\GG22457 Drosophila yakuba Dyak\GE13329 Drosophila ananassae Dana\GF11153 Drosophila pseudoobscura pseudoobscura Dpse\GA19767 Drosophila persimilis Dper\GL17077 Drosophila willistoni Dwil\GK17851 Drosophila virilis Dvir\GJ21530 Drosophila mojavensis Dmoj\GI18445 Drosophila grimshawi Dgri\GH22955
Stocks & Reagents
Stocks Listed in FlyBase ( 17 )
Bloomington	31700 y1 v1; P{TRiP.HM04006}attP2 11388 cn1 P{PZ}AGO104845/CyO; ry506 22486 y1 w67c23; P{EPgy2}AGO1EY21521 10772 y1 w67c23; P{lacW}AGO1k08121/CyO 33727 y1 sc* v1; P{TRiP.HMS00610}attP2
Harvard	- P{XP}AGO1d01402 - PBac{PB}AGO1c05033 - PBac{RB}AGO1e01877 - PBac{WH}AGO1f07079
Kyoto	113306 w*; P{GawB}AGO1NP3498 104080 w*; P{GawB}AGO1NP2077 / CyO 104194 w*; P{GawB}AGO1NP2416 / CyO
	More stocks available...
Genomic Clones ( 2 )
	BACR06M19 BACR16G12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 234 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD07701 LD26301 LD36719 LP02696 SD07283 SD07515
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT01216 AT03252 AT26777 GM10587 LD09501 LD09704 LD13711 LD17395 LD27510 LP06793 RE25327 RE35432 RE36212 SD05487 SD06679 SD08550 SD15390
Other clones	8735 10833 11000 11529 13329 14726 17258 17833 19113 28712 29185 32544 33194 34371 36870 38354 39077 45227 47281 51262 52688 56571 56713 58255 60140 62154 65855 66701 67260 69535 70691 78035 79811 83044 83427 99544 101937 102245 105103 108304 109078 119380 119831 120436 120569 120698 144325 144497 145723 146190 146286 148905 148991 153617 159704 159751 159807 160208 172446 172496 175640 192973 193003 214247.22 214289.34 214377.24 214379.13 214383.25 214541.23 214571.26 522094 527253 571191 CK01.15C.F5 CK01.15D.B6 CK01.15D.H4 CK01.52A.E6 CK01.54D.D12 CK01.59A.E8 CK01.62C.E10 CK01.70D.B10 CK01.71B.B12 CK01.71C.B6 CK01.79A.F4 CK01.96D.G4 CK01.101D.F3 CK01.122B.D8 CK01.125A.F8 CK01.130C.H3 CK01.132C.B2 CK01.132D.G7 CK01.169D.C5 Dmel_dig_006851_0623_3771 Dmel_neb_010852_0950_1677 Dmel_neb_011549_0086_1561 Dmel_neb_014092_0200_1571 Dmel_neb_014249_0166_2070 EC33524 EK015623 EK042251 EK044348 EK047937 EK056239 EK056540 EK056730 EK059538 EK061454 EK075158 EK085822 EK092940 EK093348 EK095454 EK097058 EK097802 EK101037 EK104111 EK107636 EK110924 EK111237 EK112022 EK114158 EK114505 EK114726 EK115428 EK119522 EK125736 EK129437 EK133636 EK134302 EK136136 EK137318 EK139601 EK140939 EK143540 EK147730 EK149023 EK152060 EK160840 EK167930 EK168115 EK169156 EK169235 EK177357 EK180659 EK189164 EK193531 EK194634 EK195528 EK198560 EK204901 EK208507 EK208606 EK209731 EK211711 EK216517 EK216907 EK217754 EK217838 EK222040 EK230436 EK234343 EK239064 EK240441 EK240629 EK240660 EK242748 EK243762 EK244854 EK250131 EK250249 EK252553 EK254859 EK255049 EK256959 EK262762 EK263556 EK263741 EK272521 EK272557 EK290215 EK290840 EK295921 EK296364 EK303005 EN01346 EN07552 EN16315 EP01908 EP07719 EP08038 EP08440 EP08930 EP11502 EP11814 EP15051 EP15246 EP16147 ESG013d.E21_D12 FGA010F04 FGC023F06 FGC031E10 FGH129G11 FGM208G10 FGM231E04 FGM233B01 FGM304C11 LD07513 LD25191 LD35192 RE01325 UUGC0997
RNAi & Array Information
Linkouts	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36544
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: AGO1 anon-WO0257455.29 (FlyBase, 1996-) Source for merge of: AGO1 l(2)04845 l(2)k08121 l(2)k00208 (Misra, 2000.8.25) Source for merge of: AGO1 MRE20 (FlyBase Genome Annotators, 2011)
Additional comments	MRE20 is part of the 3' UTR of AGO1. (FlyBase Genome Annotators, 2011) MRE20 corresponds to a portion of the 3' UTR of AGO1. (FlyBase Genome Annotators, 2011) Not allelic to shot. (Gregory and Brown, 1998) Source for merge of AGO1 anon-WO0257455.29 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	smg protein interacts with AGO1 protein, and AGO1 protein interacts with and is required for the translational repression of the smg target, nos mRNA. The AGO1 protein/nos mRNA interaction does not require a miRNA, but does require msg protein. (Pinder and Smibert, 2013) The miRNA strand selection of AGO1 is influenced by the presence of a 5' uridine residue in a strand of the miRNA duplex as well as thermodynamic asymmetry of the miRNA strands. (Okamura et al., 2009) Double-stranded miRNAs and siRNAs participate in a common sorting step that partitions them into AGO1- or AGO2-containing effector complexes. (Förstemann et al., 2007) The Dcr-2-r2d2 heterodimer acts as a gatekeeper for the assembly of AGO2 complexes, promoting the incorporation of siRNAs and disfavoring miRNAs as loading substrates for AGO2. A separate mechanism acts in parallel to favor miRNA/miRNA* duplexes and exclude siRNAs from assembly into AGO1 complexes. (Tomari et al., 2007) dsRNA made from templates generated with primers directed against this gene are used to infer a role for this gene in the miRNA pathway. (Behm-Ansmant et al., 2006) AGO1 is required for maintenance of long-distance chromosomal interactions between endogenous PcG target loci. (Grimaud et al., 2006) AGO1 protein associates with miRNA and cleaves target RNA completely complementary to the miRNA. (Miyoshi et al., 2005) dsRNA has been made from templates generated with primers directed against this gene. (Rehwinkel et al., 2005) AGO1 and AGO2 are not redundant. (Rehwinkel et al., 2005) Local overexpression of AGO1 can alter wing vein patterns or cause transformation of posterior cell identity. (Kataoka et al., 1999) Gene isolated in a screen of the second chromosome identifying mutants affecting disc morphology. (Roch et al., 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079010 NM_166020 NM_166021 NM_001259385 RefSeq (Proteins) • NP_523734 NP_725341 NP_725342 NP_001246314 DDBJ / EMBL / GenBank • AA264831 AA942214 AAF58313 AAF58314 AAF58315 AAK93297 AAL39684 AB035447 ABD61617 ABD61618 ABD61619 ABD61620 ABD61621 ABD61622 ABD61623 ABD61624 AFH08068 AI456633 AI534160 AQ025630 AQ025680 AQ025857 AW942837 AW943353 AX482661 AY051873 AY069539 BAA88078 CZ469697 CZ472210 CZ489849 CZ489850 DQ398917 DQ398918 DQ398919 DQ398920 DQ398921 DQ398922 DQ398923 DQ398924 Entrez Gene - A searchable database of RefSeq genes. • 36544 UniProtKB/TrEMBL • Q7KY08 Q8T059 Q27IR0 Q32KD4 Q960T1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Argonaute/Dicer protein, PAZ (IPR003100) Stem cell self-renewal protein Piwi (IPR003165) Ribonuclease H-like domain (IPR012337) Domain of unknown function DUF1785 (IPR014811)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262739 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36544
Synonyms & Secondary IDs ( 25 )
Reported As
Symbol Synonym	Ago (Du, 2005, Shcherbata et al., 2004) Ago1 (Pek and Kai, 2011, Hong et al., 2009, Liu et al., 2011, Sweeney et al., 2008, Filipowicz, 2005, Hammond, 2005, Tomari et al., 2004, Perrini et al., 2004, Mason et al., 2004, Murchison and Hannon, 2004, Skipper, 2004, Du et al., 2006, Bhadra et al., 2006, Zamore, 2007, Obbard et al., 2006, Zaratiegui, 2007, Preall and Sontheimer, 2005, Vagin et al., 2006, Valencia-Sanchez et al., 2006, Brennecke et al., 2007, Okamura et al., 2007, Tomari et al., 2007, Jacquier et al., 2008, Yang et al., 2007, Iwasaki et al., 2009, Chekulaeva et al., 2009, Zhou et al., 2008, Sabin et al., 2009, Li et al., 2009, Easow et al., 2007, Liu et al., 2007, Yang et al., 2007, Lee et al., 2009, Ameres et al., 2010, Ghildiyal et al., 2010, Shah and Förstemann, 2008, Ameres et al., 2011, Seitz et al., 2011, Iwasaki et al., 2010, Kawamata et al., 2009, Dekanty et al., 2010, Pinder and Smibert, 2013, Popkova et al., 2012, Nayak et al., 2010, Pressman et al., 2012, Hodgetts et al., 2012, Li et al., 2012, Djuranovic et al., 2012, Kawamata et al., 2011, Lindquist et al., 2011) ago1 (Azzam et al., 2012, Kavi and Birchler, 2009, Hilgers et al., 2011, Han et al., 2011, Hilgers et al., 2012, Banerjee and Slack, 2002, Hartig et al., 2009, Meyer et al., 2006, Kavi et al., 2006, Förstemann et al., 2007, Horwich et al., 2007, Flynt et al., 2009, Czech et al., 2009, Gehrke et al., 2010, Li and Lu, 2009, Helfer et al., 2012) AGO1 (Berezikov et al., 2011, Liu et al., 2011, Kolaczkowski et al., 2011, Lindquist et al., 2011, Zekri et al., 2009, Eulalio et al., 2008, Okamura et al., 2008, Miyoshi et al., 2009, Eulalio et al., 2009, Eulalio et al., 2009, FlyBase Genome Annotators, 2011, Jäger and Dorner, 2010, Grewal and Elgin, 2007, Saito et al., 2006, Dorner et al., 2006, Rehwinkel et al., 2005, Friedman and Perrimon, 2006, Gunawardane et al., 2007, Gunawardane et al., 2007, Buszczak et al., 2007, Czech et al., 2009, Kawamura et al., 2008, Molnar et al., 2006, Grieder et al., 2007, Dietzl et al., 2007, Jin et al., 2004, Eulalio et al., 2007, Saito et al., 2007, Brower-Toland et al., 2007, Minidorff et al., 2007, Rehwinkel et al., 2006, Neumuller et al., 2008, Czech et al., 2008, Okamura et al., 2008, Chung et al., 2008, Berdnik et al., 2008, Haley et al., 2008, Reich et al., 2009, Eulalio et al., 2007, Bolduc et al., 2008, Eulalio et al., 2009, Taft et al., 2009, Gesellchen et al., 2005, Kadener et al., 2009, Siomi et al., 2005, Nishida et al., 2007, Behm-Ansmant et al., 2006, Berdnik et al., 2008, Okamura et al., 2009, Flynt et al., 2010, Haas et al., 2010, Okamura et al., 2011, Wasbrough et al., 2010, Ni et al., 2011, Khong and Jan, 2011, Chekulaeva et al., 2010, Aliyari et al., 2008, Shen et al., 2011, Chung et al., 2011, Herranz et al., 2010, Westholm et al., 2012, Okamura et al., 2008) Ago-1 (Caudy et al., 2002, Bhadra et al., 2007, Kavi et al., 2006, Xu et al., 2008, Saleh et al., 2006, Mukherjee and Hanley, 2010) ago1-1 (Bernstein et al., 2001) anon-WO0257455.29   CG6671 (Jäger and Dorner, 2010, Rehwinkel et al., 2005, Molnar et al., 2006, Grieder et al., 2007, Ni et al., 2008, Ni et al., 2010.12.1) dAGO1 (Kavi and Birchler, 2009, Harris and Macdonald, 2001, Kataoka et al., 2001, Kataoka, 1999.12.2, Kataoka et al., 1999) dAgo1 (Jing et al., 2005, Denli and Hannon, 2003, Carmell et al., 2002, Gehrke et al., 2010) Dm Ago1 (Schwarz and Zamore, 2002) l(2)04845 (Spradling et al., 1999, Preston et al., 1996, Johnson-Schlitz and Engels, 2006) l(2)4845 (Gregory and Brown, 1998) l(2)k00208 (Spradling et al., 1999, Roch et al., 1998, ) l(2)k08121 (Spradling et al., 1999, Gregory and Brown, 1998, ) MRE20 (FlyBase Genome Annotators, 2011, Kageyama, 2007.2.28, Inagaki et al., 2005)
Name Synonym	Argonaute (Jaglarz et al., 2011, Antoniewski et al., 2007, Jacquier et al., 2008, Bolduc et al., 2008, Easow et al., 2007) Argonaute-1 (Zekri et al., 2009, Bhadra et al., 2006, Bhadra et al., 2007, Kavi et al., 2006, Czech et al., 2008, Berdnik et al., 2008, Eulalio et al., 2009, Herranz et al., 2010, ) Argonaute1 (Miyoshi et al., 2009, Han et al., 2011, Vagin et al., 2006, Grimaud et al., 2006, Iwasaki et al., 2009, Gesellchen et al., 2005, Ameres et al., 2010, Ghildiyal et al., 2010, Chekulaeva et al., 2010, Shen et al., 2011) Argonaute 1 (Sweeney et al., 2008, Pinder et al., 2007, Jin et al., 2004, Okamura et al., 2008, Nayak et al., 2010, Hodgetts et al., 2012) ARGONAUTE-1 (Bhadra et al., 2006) argonaute-1 (Obbard et al., 2006.2.10) argonaute 1 (Gehrke et al., 2010) Argonaute protein 1 (Yang et al., 2007) mRNA-like ncRNA in embryogenesis 20
Secondary FlyBase IDs
	FBgn0010586 FBgn0022056 FBgn0022275 FBgn0026611 FBgn0063793 FBgn0086551
References ( 216 )
Generate a list of	All references relating to this gene ( 216 )
List References by type	Research paper  ( 139 ) Supplementary material  ( 11 ) Review  ( 30 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 12 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 3 ) Patent  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 35 )
	Flores-Jasso et al., 2013, RNA 19(2): 271--279 Rapid and specific purification of Argonaute-small RNA complexes from crude cell lysates. [FBrf0221077] Pinder and Smibert, 2013, EMBO Rep. 14(1): 80--86 microRNA-independent recruitment of Argonaute 1 to nanos mRNA through the Smaug RNA-binding protein. [FBrf0220359] Azzam et al., 2012, Dev. Biol. 365(2): 384--394 Drosophila Argonaute 1 and its miRNA biogenesis partners are required for oocyte formation and germline cell division. [FBrf0218153] Backes et al., 2012, Cell Host Microbe 12(2): 200--210 Degradation of host microRNAs by poxvirus poly(A) polymerase reveals terminal RNA methylation as a protective antiviral mechanism. [FBrf0220695] Djuranovic et al., 2012, Science 336(6078): 237--240 miRNA-mediated gene silencing by translational repression followed by mRNA deadenylation and decay. [FBrf0218066] Fukaya and Tomari, 2012, Mol. Cell 48(6): 825--836 MicroRNAs Mediate Gene Silencing via Multiple Different Pathways in Drosophila. [FBrf0220346] Helfer et al., 2012, PLoS ONE 7(1): e28907 AU-Rich Element-Mediated mRNA Decay Can Occur Independently of the miRNA Machinery in Mouse Embryonic Fibroblasts and Drosophila S2-Cells. [FBrf0217194] Hilgers et al., 2012, Genes Dev. 26(20): 2259--2264 ELAV mediates 3' UTR extension in the Drosophila nervous system. [FBrf0219699] Hodgetts et al., 2012, Genome 55(4): 312--326 An intact RNA interference pathway is required for expression of the mutant wing phenotype of vg(21-3), a P-element-induced allele of the vestigial gene in Drosophila. [FBrf0217920] Li et al., 2012, Development 139(9): 1547--1556 Mei-P26 regulates the maintenance of ovarian germline stem cells by promoting BMP signaling. [FBrf0218009] Menon and Meller, 2012, Genetics 191(3): 1023--1028 A Role for siRNA in X-Chromosome Dosage Compensation in Drosophila melanogaster. [FBrf0218921] Michalik et al., 2012, Nucleic Acids Res. 40(19): 9596--9603 A small RNA response at DNA ends in Drosophila. [FBrf0219708] Moretti et al., 2012, Nat. Struct. Mol. Biol. 19(6): 603--608 PABP and the poly(A) tail augment microRNA repression by facilitated miRISC binding. [FBrf0218529] Popkova et al., 2012, PLoS Genet. 8(12): e1003159 Polycomb controls gliogenesis by regulating the transient expression of the gcm/glide fate determinant. [FBrf0220515] Pressman et al., 2012, G3 (Bethesda) 2(4): 437--448 A Systematic Genetic Screen to Dissect the MicroRNA Pathway in Drosophila. [FBrf0218180] Westholm et al., 2012, RNA 18(2): 177--192 Common and distinct patterns of terminal modifications to mirtrons and canonical microRNAs. [FBrf0217201] Ameres et al., 2011, RNA 17(1): 54--63 Target RNA-directed tailing and trimming purifies the sorting of endo-siRNAs between the two Drosophila Argonaute proteins. [FBrf0212657] Berezikov et al., 2011, Genome Res. 21(2): 203--215 Deep annotation of Drosophila melanogaster microRNAs yields insights into their processing, modification, and emergence. [FBrf0213080] Chung et al., 2011, Genome Res. 21(2): 286--300 Computational and experimental identification of mirtrons in Drosophila melanogaster and Caenorhabditis elegans. [FBrf0213087] Fukaya and Tomari, 2011, EMBO J. 30(24): 4998--5009 PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro. [FBrf0216898] Han et al., 2011, Curr. Biol. 21(22): 1878--1887 The 3'-to-5' Exoribonuclease Nibbler Shapes the 3' Ends of MicroRNAs Bound to Drosophila Argonaute1. [FBrf0216729] Hilgers et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(38): 15864--15869 Neural-specific elongation of 3' UTRs during Drosophila development. [FBrf0215804] Jaglarz et al., 2011, Cell Tissue Res. 344(1): 169--181 Nuage morphogenesis becomes more complex: two translocation pathways and two forms of nuage coexist in Drosophila germline syncytia. [FBrf0213313] Kawamata et al., 2011, EMBO Rep. 12(9): 944--949 Multilayer checkpoints for microRNA authenticity during RISC assembly. [FBrf0218100] Khong and Jan, 2011, J. Virol. 85(4): 1439--1451 Modulation of Stress Granules and P Bodies during Dicistrovirus Infection. [FBrf0212847] Kibanov et al., 2011, Mol. Biol. Cell 22(18): 3410--3419 A novel organelle, the piNG-body, in the nuage of Drosophila male germ cells is associated with piRNA-mediated gene silencing. [FBrf0215572] Kolaczkowski et al., 2011, Mol. Biol. Evol. 28(2): 1033--1042 Recurrent adaptation in RNA interference genes across the Drosophila phylogeny. [FBrf0212798] Lindquist et al., 2011, Genome Res. 21(3): 433--446 Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling. [FBrf0213199] Liu et al., 2011, Development 138(9): 1863--1873 PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition. [FBrf0213491] Liu et al., 2011, Curr. Biol. 21(22): 1888--1893 The Exoribonuclease Nibbler Controls 3' End Processing of MicroRNAs in Drosophila. [FBrf0216782] Ni et al., 2011, Nat. Methods 8(5): 405--407 A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0213581] Okamura et al., 2011, Mol. Cell. Biol. 31(4): 884--896 R2D2 Organizes Small Regulatory RNA Pathways in Drosophila. [FBrf0212879] Pek and Kai, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 12007--12012 DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation. [FBrf0214405] Seitz et al., 2011, Silence 2: 4 A 5'-uridine amplifies miRNA/miRNA* asymmetry in Drosophila by promoting RNA-induced silencing complex formation. [FBrf0214042] Shen et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(38): 15942--15947 Testing hypotheses on the rate of molecular evolution in relation to gene expression using microRNAs. [FBrf0216936] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...