General Information
Symbol
Dmel\Fs(2)Ket
Species
D. melanogaster
Name
Female sterile (2) Ketel
Annotation Symbol
CG2637
Feature Type
FlyBase ID
FBgn0262743
Gene Model Status
Stock Availability
Gene Snapshot
Female sterile (2) Ketel (Fs(2)Ket) functions in nuclear protein import, formation of the spindle apparatus and nuclear envelope assembly. Fs(2)Ket is also involved in biogenesis of the mitochondria. [Date last reviewed: 2016-09-01]
Also Known As
Ketel, MRE26, 38E.11, imp-β, fs(2)lto2
Genomic Location
Cytogenetic map
Sequence location
2L:20,734,609..20,750,941 [+]
Recombination map
2-54
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the importin beta family. Importin beta-1 subfamily. (O18388)
Molecular Function (see GO section for details)
Experimental Evidence
Predictions / Assertions
Summaries
Gene Group Membership
BETA IMPORTINS -
Importin-β family proteins are nucleocytoplasmic transport receptors that import and export proteins and RNAs through the nuclear pores. In some import/export pathways, importin-β directly interacts with the cargo. For cargo containing a classical nuclear localization (cNLS) signal, importin-α acts as an adaptor linking the cargo to importin-β. (Adapted from FBrf0235243, FBrf0131350 and PMID:21029754).
UniProt Contributed Function Data
Required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins (PubMed:11102382). In Drosophila, may not function as a snRNP import receptor as it does not interact with components of the snRNP complex such as snRNP U1, U2, U4/U6 and Snup (PubMed:23885126).
(UniProt, O18388)
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Fs(2)Ket or the JBrowse view of Dmel\Fs(2)Ket for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.47
Large number of splice variants within 3' UTR (most not annotated); correlates with presence of transposable element within 3' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.48
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0081392
3601
884
FBtr0305584
3783
884
FBtr0305585
5571
884
FBtr0305586
6818
884
FBtr0305587
4699
884
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0080922
98.7
884
4.66
FBpp0294035
98.7
884
4.66
FBpp0294036
98.7
884
4.66
FBpp0294037
98.7
884
4.66
FBpp0294038
98.7
884
4.66
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Forms a complex with an importin alpha subunit (By similarity). Interacts with the sesquiterpenoid juvenile hormone receptor Met (PubMed:27979731).
(UniProt, O18388)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Fs(2)Ket using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (15 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from physical interaction with UniProtKB:Q9VYW2
(assigned by UniProt )
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000055427
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR001494
(assigned by InterPro )
Biological Process (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from high throughput mutant phenotype
inferred from high throughput mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from genetic interaction with FLYBASE:Pen; FB:FBgn0267727
inferred from mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000055427
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000055427
(assigned by GO_Central )
Cellular Component (7 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from physical interaction with FLYBASE:tud; FB:FBgn0003891
inferred from physical interaction with FLYBASE:vas; FB:FBgn0283442
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000055427
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000055427
(assigned by GO_Central )
inferred from sequence or structural similarity with EMBL:L38951
inferred from sequence or structural similarity with protein_id:AAC41763
Expression Data
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Western blot analysis reveals that Fs(2)Ket protein is expressed most strongly in embryos, and in the ovaries of adult females, and is expressed at all developmental stages. In third instar larvae, Western analysis detects Fs(2)Ket protein in the imaginal discs, the central nervous system, and faintly in the fat-body. Very little or no expression is observed in the salivary glands, gut, Malpighian tubules, epidermis or muscle. Fs(2)Ket protein is cytoplasmic in all cell types in which it is expressed, and is expressed predominantly in mitotically active cells.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from high throughput direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from physical interaction with FLYBASE:tud; FB:FBgn0003891
inferred from physical interaction with FLYBASE:vas; FB:FBgn0283442
Expression Deduced from Reporters
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Fs(2)Ket in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 45 )
For All Classical and Insertion Alleles Show
 
Allele of Fs(2)Ket
Class
Mutagen
Associated Insertion
Stocks
Known lesion
Other relevant insertions
insertion of mobile activating element
Name
Expression Data
miscellaneous insertions
Name
Expression Data
Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Fs(2)Ket
Allele of Fs(2)Ket
Mutagen
Associated Transgenic Construct
Stocks
Transgenic constructs containing regulatory region of Fs(2)Ket
Deletions and Duplications ( 40 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
12 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
9 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
12 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (3)
9 of 9
Yes
Yes
5 of 9
No
Yes
5 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
15 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
12 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091901ZY )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501D1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W015J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X013W )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01XQ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 2 )
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Forms a complex with an importin alpha subunit (By similarity). Interacts with the sesquiterpenoid juvenile hormone receptor Met (PubMed:27979731).
    (UniProt, O18388 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-54
    Cytogenetic map
    Sequence location
    2L:20,734,609..20,750,941 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    38E5-38E6
    Limits computationally determined from genome sequence between P{PZ}Hr3802306&P{PZ}dia1 and P{EP}CG31673EP432
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    38E1-38E3
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Notes
    Stocks and Reagents
    Stocks (18)
    Genomic Clones (18)
    cDNA Clones (66)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: Fs(2)Ket CG2637
      Source for database merge of
      Source for merge of: Fs(2)Ket fs(2)lto2
      Source for merge of: Fs(2)Ket MRE26
      Additional comments
      MRE26 is part of the 3' UTR of Fs(2)Ket.
      MRE26 corresponds to a portion of the 3' UTR of Fs(2)Ket.
      Other Comments
      Expression is enriched in embryonic gonads.
      RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
      RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 cells: change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions. Alterations of cell shape are also evident in S2R+ cells.
      Fs(2)Ket gene product is maternally provided and is required during both embryogenesis and larval life. The gene product is involved in nuclear protein import.
      Fs(2)Ket mutant phenotype includes ventralised eggs and 'topless' embryos.
      Fs(2)Ket mutant phenotypes suggest the involvement of the normal Fs(2)Ket gene product in cell communication.
      Mutations at the fs(2)lto2 locus cause defects in midoogenesis.
      Origin and Etymology
      Discoverer
      Etymology
      Named for a Hungarian family that vanished by the beginning of the 14th century.
      Identification
      External Crossreferences and Linkouts ( 52 )
      Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Linkouts
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
      DroID - A comprehensive database of gene and protein interactions.
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      KEGG Genes - Molecular building blocks of life in the genomic space.
      Synonyms and Secondary IDs (38)
      Reported As
      Symbol Synonym
      Fs(2)Hon
      Fs(2)Sz6
      Fs(2)Sz7
      fs(2)lo-B
      fs(2)ltoHD43
      Secondary FlyBase IDs
      • FBgn0000986
      • FBgn0000984
      • FBgn0000989
      • FBgn0004812
      • FBgn0005392
      • FBgn0086557
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (136)