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Gene Dmel\S6kII

General Information
Symbol	Dmel\S6kII	Species	D. melanogaster
Name	Ribosomal protein S6 kinase II	Annotation symbol	CG17596
Feature type	protein_coding_gene	FlyBase ID	FBgn0262866
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	RSK, ign, rsk2, dRSK
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	20C1-20C1	Sequence location	X:21,931,800..21,938,207 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ribosomal protein S6 kinase II is referred to in FlyBase by the symbol Dmel\S6kII (CG17596, FBgn0262866). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; ribosomal protein S6 kinase activity. There is experimental evidence for 11 unique biological process terms, many of which group under: biological regulation; regulation of developmental process; single-organism developmental process; learning or memory; response to stimulus; system process; cognition; cellular component organization or biogenesis; associative learning; determination of adult lifespan; post-embryonic organ morphogenesis; locomotory behavior. 29 alleles are reported. The phenotypes of these alleles are annotated with: rhabdomere; retina; interommatidial bristle; cone cell; mesothoracic tergum; eye photoreceptor cell; ommatidial precursor cluster; ommatidium; wing vein; pigment cell. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: AGC-kinase, C-terminal; Protein kinase, ATP binding site; Protein kinase, C-terminal; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Serine/threonine-protein kinase, active site. Gene sequence location is X:21931800..21938207.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 20C1-20C1   Limits computationally determined from genome sequence between P{EP}CG1702EP1525 and P{EP}EP1465&P{EP}CG1486EP1192
Experimentally Determined Cytological Location
Cytogenetic map Notes References 20-20     (Wassarman, 1994.10.14) 20-20   (determined by in situ hybridisation)   (Wassarman et al., 1994)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\S6kII for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) S6kII-RA FBtr0077360  NM_078713   3777   911 S6kII-RB FBtr0333715  NM_001272850   6055   911
Additional Transcript Data & Comments
Reported size (kB)	3.137 (longest cDNA) (Wassarman et al., 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein S6kII-PA   FBpp0077052   100.4   911   6.95     NP_523437   AAF50945 S6kII-PB   FBpp0305864   100.4   911   6.95     NP_001259779   AGB95617
Additional Polypeptide Data & Comments
Reported size (kDa)	910 (aa); 100 (kD predicted) (Wassarman et al., 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) AGC-kinase, C-terminal (IPR000961) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Protein kinase, C-terminal (IPR017892)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014298 AAF50945   AE014298 AGB95617   AI109152   AI402571   AI533371   AW944407   AY231889   BF502631   BI166291   BI239396   BT004472 AAO42636   CO154731   CO266850   CO269023   CO269892   CO271716   CO274117   CO285870   CO286586   CO289981   CO318405   CO343270   EC053320   EC054882   EC055345   EC056288   EC063374   EC069525   EC083022   EC197266   EC198100   EC208587   EC210474   EC218325   EC219150   EC246204   EC250108   EC257814   EC261944   L28945 AAA50509
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VR61 Q86NP0 Q24496
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
neuromuscular junction inferred from direct assay (Fischer et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    5 central nervous system, pupae P8    6 head, virgin 1-day female    4 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    9 ovary, mated 4-day female    6 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    5 central nervous system, pupae P8    6 head, virgin 1-day female    4 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    9 ovary, mated 4-day female    6 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    5 central nervous system, pupae P8    6 head, virgin 1-day female    4 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    9 ovary, mated 4-day female    6 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    5 central nervous system, pupae P8    6 head, virgin 1-day female    4 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    9 ovary, mated 4-day female    6 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    5 central nervous system, pupae P8    6 head, virgin 1-day female    4 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    9 ovary, mated 4-day female    6 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    5 central nervous system, pupae P8    6 head, virgin 1-day female    4 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    9 ovary, mated 4-day female    6 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    5 central nervous system, pupae P8    6 head, virgin 1-day female    4 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    9 ovary, mated 4-day female    6 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    3 central nervous system, larvae L3    5 central nervous system, pupae P8    6 head, virgin 1-day female    4 head, virgin 4-day female    2 head, virgin 20-day female    3 head, mated 1-day female    3 head, mated 4-day female    4 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    6 head, mated 20-day male    6 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    9 ovary, mated 4-day female    6 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    9 embryo 04-06hr    12 embryo 06-08hr    13 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    4 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    9 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    5 adult female 05day    8 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    9 embryo 04-06hr    12 embryo 06-08hr    13 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    4 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    9 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    5 adult female 05day    8 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    9 embryo 04-06hr    12 embryo 06-08hr    13 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    4 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    9 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    5 adult female 05day    8 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    9 embryo 04-06hr    12 embryo 06-08hr    13 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    4 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    9 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    5 adult female 05day    8 adult female 30day    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    9 embryo 04-06hr    12 embryo 06-08hr    13 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    4 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    9 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    5 adult female 05day    8 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    9 embryo 04-06hr    12 embryo 06-08hr    13 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    4 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    9 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    5 adult female 05day    8 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    9 embryo 04-06hr    12 embryo 06-08hr    13 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    4 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    9 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    5 adult female 05day    8 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    17 embryo 02-04hr    9 embryo 04-06hr    12 embryo 06-08hr    13 embryo 08-10hr    12 embryo 10-12hr    10 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    4 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    9 white prepupae new    6 white prepupae 12hr    5 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    3 adult female 01day    5 adult female 05day    8 adult female 30day    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    6 embryonic 1182-4H    5 embryonic GM2    9 embryonic Kc167    15 embryonic S1    4 embryonic S3    4 leg disc CME L1    5 wing disc CME-W2    5 wing disc ML-DmD8    4 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    4 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    3 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    3 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    6 embryonic 1182-4H    5 embryonic GM2    9 embryonic Kc167    15 embryonic S1    4 embryonic S3    4 leg disc CME L1    5 wing disc CME-W2    5 wing disc ML-DmD8    4 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    4 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    3 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    3 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    6 embryonic 1182-4H    5 embryonic GM2    9 embryonic Kc167    15 embryonic S1    4 embryonic S3    4 leg disc CME L1    5 wing disc CME-W2    5 wing disc ML-DmD8    4 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    4 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    3 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    3 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    6 embryonic 1182-4H    5 embryonic GM2    9 embryonic Kc167    15 embryonic S1    4 embryonic S3    4 leg disc CME L1    5 wing disc CME-W2    5 wing disc ML-DmD8    4 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    4 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    3 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    3 ovary OSC    4 ovary OSS    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    6 embryonic 1182-4H    5 embryonic GM2    9 embryonic Kc167    15 embryonic S1    4 embryonic S3    4 leg disc CME L1    5 wing disc CME-W2    5 wing disc ML-DmD8    4 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    4 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    3 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    3 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    6 embryonic 1182-4H    5 embryonic GM2    9 embryonic Kc167    15 embryonic S1    4 embryonic S3    4 leg disc CME L1    5 wing disc CME-W2    5 wing disc ML-DmD8    4 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    4 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    3 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    3 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    6 embryonic 1182-4H    5 embryonic GM2    9 embryonic Kc167    15 embryonic S1    4 embryonic S3    4 leg disc CME L1    5 wing disc CME-W2    5 wing disc ML-DmD8    4 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    4 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    3 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    3 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    6 embryonic 1182-4H    5 embryonic GM2    9 embryonic Kc167    15 embryonic S1    4 embryonic S3    4 leg disc CME L1    5 wing disc CME-W2    5 wing disc ML-DmD8    4 wing disc ML-DmD9    4 wing disc ML-DmD16-c3    5 wing disc ML-DmD21    4 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    5 eye-antennal disc ML-DmD11    5 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    3 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    3 ovary OSC    4 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    6 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    6 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    6 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    6 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    6 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    6 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    6 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    6 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_01 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable S6kIID1 viable, with Scer\GAL4pnr-MD237 S6kIIGD1254 Sterility Allele fertile | reduced S6kIID1 Other Phenotypes Allele learning defective S6kIIign-Δ30-2 S6kIIt6.5 learning defective (with Df(1)R29) S6kIIign-Δ24-3 learning defective (with S6kIIign-Δ24-3) S6kIIign-Δ58-1 learning defective (with S6kIIign-Δ58-1) S6kIIign-Δ24-3 learning defective | dominant S6kIIign-Δ24-3 learning defective | male limited S6kIIign-P1 locomotor rhythm defective S6kIIign-Δ58-1 memory defective S6kIIign-Δ58-1 memory defective | male limited S6kIIign-P1 memory defective | recessive S6kIIign-Δ24-3 neuroanatomy defective | larval stage S6kIIScer\UAS.cRa neuroanatomy defective | third instar larval stage S6kIIign-Δ24-3 S6kIIign-Δ58-1 short lived S6kIID1 visible, with Scer\GAL4GMR.PF S6kIIScer\UAS.cRa S6kIIScer\UAS.T:Hsap\MYC visible, with Scer\GAL4pnr-MD237 S6kIIGD1254 visible (with Df(1)R8A) S6kIID1 visible | recessive S6kIID1 Phenotype manifest in Allele chaeta | supernumerary, with Scer\GAL4pnr-MD237 S6kIIGD1254 cone cell S6kIID1 cone cell (with Df(1)R8A) S6kIID1 eye photoreceptor cell S6kIID1 eye photoreceptor cell, with Scer\GAL4hs.2sev S6kIIK231R.Scer\UAS.T:Hsap\MYC eye photoreceptor cell (with Df(1)R8A) S6kIID1 interommatidial bristle, with Scer\GAL4GMR.PF S6kIIScer\UAS.T:Hsap\MYC mesothoracic tergum, with Scer\GAL4pnr-MD237 S6kIIGD1254 NMJ bouton | larval stage S6kIIScer\UAS.cRa NMJ bouton | third instar larval stage S6kIIign-Δ58-1 S6kIIign-Δ24-3 ommatidial precursor cluster S6kIID1 ommatidial precursor cluster (with Df(1)R8A) S6kIID1 ommatidium, with Scer\GAL4GMR.PF S6kIIScer\UAS.T:Hsap\MYC S6kIIScer\UAS.cRa pigment cell S6kIID1 pigment cell (with Df(1)R8A) S6kIID1 retina, with Scer\GAL4GMR.PF S6kIIK231M.K597M.Scer\UAS.T:Hsap\MYC S6kIIK231R.Scer\UAS.T:Hsap\MYC S6kIIK231R.K597R.Scer\UAS.T:Hsap\MYC rhabdomere, with Scer\GAL4GMR.PF S6kIIScer\UAS.T:Hsap\MYC wing vein, with Scer\GAL4Bx-MS1096 S6kIIScer\UAS.T:Hsap\MYC wing vein, with Scer\GAL4en-e16E S6kIIK231M.K597M.Scer\UAS.T:Hsap\MYC S6kIIK231R.Scer\UAS.T:Hsap\MYC S6kIIScer\UAS.T:Hsap\MYC S6kIIK231R.K597R.Scer\UAS.T:Hsap\MYC wing vein | ectopic S6kIID1 wing vein | ectopic (with Df(1)R8A) S6kIID1
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of S6kII Class Mutagen Stocks Known lesion S6kIIG1845 P-element activity 1 -- S6kIID1 loss of function allele Delta2-3 0 Yes S6kIIEP7363 P-element activity 0 Yes S6kIIign-P1 P-element activity 0 Yes S6kIIign-Δ1P1 P-element activity 0 Yes S6kIIign-Δ1P2 P-element activity 0 Yes S6kIIign-Δ24-3 P-element activity 0 Yes S6kIIign-Δ30-2 P-element activity 0 Yes S6kIIign-Δ58-1 loss of function allele P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 20 )
For All Alleles Carried on Transgenic Constructs Show
Allele of S6kII Class Mutagen Stocks Known lesion S6kIIGD1254 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes S6kIIGL00207 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes S6kIIJF02814 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes S6kIIKK109199 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes S6kIIScer\UAS.cBa in vitro construct - regulatory fusion 1 Yes S6kIIK213R.R902A.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis in vitro construct - deletion 0 Yes S6kIIK231M.K597M.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis in vitro construct - deletion 0 Yes S6kIIK231R.K597R.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis in vitro construct - deletion 0 Yes S6kIIK231R.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis in vitro construct - deletion 0 Yes S6kIIK597M.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes S6kIINIG.17596R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes S6kIIR902A.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis in vitro construct - deletion 0 Yes S6kIIS515A.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes S6kIIS515D.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes S6kIIScer\UAS.cRa in vitro construct - regulatory fusion 0 Yes S6kIIScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes S6kIIt6.5 in vitro construct - genomic fragment 0 Yes S6kIIT732A.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes S6kIIT732E.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes S6kIIT:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)S6kII-ignΔ67-1 (Putz et al., 2004)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1254} NA P{KK109199} NA P{NIG.17596R} NA P{TRiP.GL00207} NA P{TRiP.JF02814} NA P{UAS-RSK.K213R.R902A} NA P{UAS-RSK.K231M.K597M} NA P{UAS-RSK.K231R.K597R} NA P{UAS-RSK.K231R} NA P{UAS-RSK.R902A} NA P{UAS-RSK.WT.MYC} NA P{UAS-S6kII.B} NA P{UAS-S6kII.K597M} NA P{UAS-S6kII.R} NA P{UAS-S6kII.S515A} NA P{UAS-S6kII.S515D} NA P{UAS-S6kII.T732A} NA P{UAS-S6kII.T732E} NA characterization construct P{gen-GFP-RSK} NA P{S6kIIt6.5} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}S6kIIEP7363 NA P{EP}S6kIIG1845 NA insertion of enhancer trap P{lacW}S6kIIign-P1 No miscellaneous insertions P{?lacW}S6kIIign-Δ24-3 NA P{?lacW}S6kIIign-Δ30-2 NA P{?lacW}S6kIIign-Δ58-1 NA
Gene Ontology: Function, Process & Cellular Component ( 18 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with rl (Kim et al., 2006) ribosomal protein S6 kinase activity inferred from direct assay (Kim et al., 2006)
Biological Process
CV term Evidence References
associative learning inferred from mutant phenotype (Putz et al., 2004) determination of adult lifespan inferred from mutant phenotype (Kim et al., 2006) imaginal disc-derived wing vein specification inferred from mutant phenotype (Kim et al., 2006) locomotor rhythm inferred from mutant phenotype (Akten et al., 2009) negative regulation of compound eye cone cell fate specification inferred from mutant phenotype (Kim et al., 2006) negative regulation of photoreceptor cell differentiation inferred from mutant phenotype (Kim et al., 2006) negative regulation of pigment cell differentiation inferred from mutant phenotype (Kim et al., 2006) negative regulation of protein import into nucleus inferred from direct assay (Kim et al., 2006) negative regulation of Ras protein signal transduction inferred from genetic interaction with phl (Kim et al., 2006) inferred from genetic interaction with Ras85D (Kim et al., 2006) neuromuscular junction development inferred from mutant phenotype (Fischer et al., 2009) operant conditioning inferred from mutant phenotype (Putz et al., 2004)
Cellular Component
CV term Evidence References
neuromuscular junction inferred from direct assay (Fischer et al., 2009)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR002290, InterPro:IPR017441, InterPro:IPR017892 (FlyBase Curators et al., 2004-) protein serine/threonine kinase activity non-traceable author statement (Morrison et al., 2000) ribosomal protein S6 kinase activity inferred from sequence or structural similarity (Wassarman et al., 1994) non-traceable author statement (Wassarman, 1994.10.14)
Biological Process
CV term Evidence References
associative learning traceable author statement (Brembs, 2003) operant conditioning traceable author statement (Brembs, 2003) protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR002290, InterPro:IPR008271, InterPro:IPR017892 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Wassarman et al., 1994) non-traceable author statement (Morrison et al., 2000)
Cellular Component
CV term Evidence References
ribosome inferred from sequence or structural similarity (Wassarman et al., 1994) non-traceable author statement (Wassarman, 1994.10.14)
Sequence Ontology: Class of Gene
	gene (Olson et al., 2007) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions S6kII allele Gene References S6kIIScer\UAS.cRa Pdk1 (Rintelen et al., 2001) unspecified Dsor1 (Kim et al., 2006) phl (Kim et al., 2006) Ras85D (Kim et al., 2006) rl (Kim et al., 2006) sev (Kim et al., 2006)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262866 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q24496
Orthologs
OrthoDB Orthologs (56) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6JQ4GW Dipteran inclusive ortholog search EOG6PZJFN Insect inclusive ortholog search EOG6D51SW Arthropod inclusive ortholog search EOG6QZ624 Metazoa inclusive ortholog search EOG61CKPD
Orthologs in Drosophila Species (EOG6JQ4GW)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\S6kII     Drosophila simulans   Dsim\GD17550 Y   Drosophila sechellia   Dsec\GM11736 Y   Drosophila erecta   Dere\GG19750 Y   Drosophila yakuba   Dyak\S6kII Y   Drosophila ananassae   Dana\GF21004 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA14570 Y   Drosophila persimilis   Dper\GL13301 Y   Drosophila willistoni   Dwil\GK25774 Y   Drosophila virilis   Dvir\GJ18812 Y   Drosophila mojavensis   Dmoj\GI14475 Y   Drosophila grimshawi   Dgri\GH24077 Y
Orthologs in non-Drosophila Dipterans (EOG6PZJFN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004474   Anopheles gambiae Malaria mosquito  Agam\AGAP003040   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ014359
Orthologs in non-Dipteran Insects (EOG6D51SW)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\S6kII   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG013142   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13790   Atta cephalotes Leafcutter ant  Acep\ACEP19240   Camponotus floridanus Florida carpenter ant  Cflo\CFLO13262   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16934   Linepithema humile Argentine ant  Lhum\LH19909   Pogonomyrmex barbatus Red harvester ant  Pbar\PB12168   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004772   Bombyx mori Silkmoth  Bmor\BGIBMGA011088   Pediculus humanus Human body louse  Phum\PHUM599900   Tribolium castaneum Red flour beetle  Tcas\TC001074
Orthologs in non-Insect Arthropods (EOG6QZ624)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Ammecr1l
Orthologs in non-Arthropod Metazoa (EOG61CKPD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\rskn-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011714gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020782gn   Danio rerio Zebrafish  Drer\BX255965.1   Danio rerio Zebrafish  Drer\ENSDARG00000037574   Danio rerio Zebrafish  Drer\OTTDARG00000028759   Danio rerio Zebrafish  Drer\rps6ka1   Danio rerio Zebrafish  Drer\rps6ka3a   Xenopus tropicalis Western clawed frog  Xtro\rps6ka3   Xenopus tropicalis Western clawed frog  Xtro\rps6ka2   Xenopus tropicalis Western clawed frog  Xtro\rps6ka1   Xenopus tropicalis Western clawed frog  Xtro\rps6ka6   Gallus gallus Domestic chicken  Ggal\RPS6KA2   Gallus gallus Domestic chicken  Ggal\RPS6KA6   Gallus gallus Domestic chicken  Ggal\RPS6KA   Gallus gallus Domestic chicken  Ggal\RPS6KA3   Mus musculus House mouse  Mmus\Rps6ka6   Mus musculus House mouse  Mmus\Rps6ka3   Mus musculus House mouse  Mmus\Rps6ka2   Mus musculus House mouse  Mmus\Rps6ka1   Rattus norvegicus Norway rat  Rnor\Rps6ka3   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000002592   Rattus norvegicus Norway rat  Rnor\Rps6ka1   Rattus norvegicus Norway rat  Rnor\Rps6ka2   Homo sapiens Human  Hsap\RPS6KA3   Homo sapiens Human  Hsap\RPS6KA1   Homo sapiens Human  Hsap\RPS6KA6   Homo sapiens Human  Hsap\RPS6KA2
Human Orthologs (4)
Gene OMIM HGNC Hsap\RPS6KA3     Hsap\RPS6KA1     Hsap\RPS6KA6     Hsap\RPS6KA2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD17550 Drosophila sechellia Dsec\GM11736 Drosophila erecta Dere\GG19750 Drosophila yakuba Dyak\S6kII Drosophila ananassae Dana\GF21004 Drosophila pseudoobscura pseudoobscura Dpse\GA14570 Drosophila persimilis Dper\GL13301 Drosophila willistoni Dwil\GK25774 Drosophila virilis Dvir\GJ18812 Drosophila mojavensis Dmoj\GI14475 Drosophila grimshawi Dgri\GH24077
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	8714 w1118; P{UAS-S6kII.B}85/TM3, Sb1 32601 w* P{EP}S6kIIG1845 CG17600G1845 35583 y1 sc* v1; P{TRiP.GL00207}attP2 27731 y1 v1; P{TRiP.JF02814}attP2/TM3, Sb1
VDRC	v101451 P{KK109199}VIE-260B v5702 w1118; P{GD1254}v5702
Genomic Clones ( 2 )
	BACR05K22 BACR06L03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 34 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD05277
Other clones	yak-em_S6kII
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT18425 GH08264 GH21818 RE06367 RE35874
Other clones	12041 36894 59287 66677 166784 214309.64 524062 CK01.60A.H10 CK01.93A.E8 CK01.122B.C4 CK01.125C.C10 CK01.170B.G5 EC053320 EC054882 EC055345 EC056288 EK061036 EK063263 EK072752 EK093342 EK096243 EK104427 EK113222 EK147746 EK271957 EN04664 EP16435
RNAi & Array Information
Linkouts	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33139
Antibody Information
	polyclonal (Fischer et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: S6kII rsk2 (Restifo, 2011.1.20)
Additional comments
Other Comments
	S6kII negatively regulates bouton number at the larval neuromuscular junction. (Fischer et al., 2009) Homologue of human RSK2 corresponding to Coffin-Lowry syndrome gene. (Olson et al., 2007) The rl-binding activity of S6kII, but not the phosphotransferase activity, is essential for the in vivo function of S6kII in eye and wing development. S6kII regulates rl activity by acting as a cytoplasmic anchor. (Kim et al., 2006) When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G2/M phase cells is seen. (Bettencourt-Dias et al., 2004) One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders. (Inlow and Restifo, 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) A S6kII cDNA has been cloned and sequenced. (Wassarman et al., 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078713 NM_001272850 RefSeq (Proteins) • NP_523437 NP_001259779 DDBJ / EMBL / GenBank • AAA50509 AAF50945 AAF50945.1 AAO42636 AGB95617 AI533371 AW944407 BT004472 L28945 Entrez Gene - A searchable database of RefSeq genes. • 33139 UniProtKB/TrEMBL • Q9VR61 Q86NP0 Q24496
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) AGC-kinase, C-terminal (IPR000961) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Protein kinase, C-terminal (IPR017892)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262866 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33139 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q24496
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	CG17596 (Sun et al., 2010, Morrison et al., 2000, Putz et al., 2004, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2011.7.19) dRSK (Pospisilik et al., 2010, Rintelen et al., 2001) ign (Brembs, 2003, Putz et al., 2004, Neuser et al., 2008) RSK (Bjorklund et al., 2006, Brembs, 2003, Morrison et al., 2000, Johansen et al., 1999, Wassarman, 1994.10.14, Kim et al., 2006, Akten et al., 2009) Rsk   rsk (Fischer et al., 2009) rsk2 (Restifo, 2011.1.20, Olson et al., 2007) S6KII (Slack et al., 2010, Wassarman et al., 1994, Putz et al., 2004, Akten et al., 2009, Neuser et al., 2008, Tangredi et al., 2012) S6kII (Sun et al., 2010, Restifo, 2011.1.20, Akten et al., 2009, Korcsmáros et al., 2011, Japanese National Institute of Genetics, 2012.5.21)
Name Synonym	ignorant (Brembs, 2003, Putz et al., 2004, Neuser et al., 2008) p90 ribosomal S6 kinase (Brembs, 2003, Kim et al., 2006, Akten et al., 2009) Ribosomal protein S6 kinase II   ribosomal-S6-kinase (Pospisilik et al., 2010) Ribosomal-S6-kinase   RPS6-protein kinase-II   S6 kinase II (Neuser et al., 2008)
Secondary FlyBase IDs
	FBgn0011285 FBgn0086241
References ( 45 )
Generate a list of	All references relating to this gene ( 45 )
List References by type	Research paper  ( 19 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) Letter  ( 1 )
Recent research papers ( 4 )
	Kuntz et al., 2012, Learn. Mem. 19(8): 337--340 The visual orientation memory of Drosophila requires Foraging (PKG) upstream of Ignorant (RSK2) in ring neurons of the central complex. [FBrf0218963] Tangredi et al., 2012, J. Biol. Chem. 287(20): 16748--16758 The C-terminal Kinase and ERK-binding Domains of Drosophila S6KII (RSK) Are Required for Phosphorylation of the Protein and Modulation of Circadian Behavior. [FBrf0218308] Booker et al., 2011, BMC Genomics 12: 50 False negative rates in Drosophila cell-based RNAi screens: a case study. [FBrf0212969] Korcsmáros et al., 2011, PLoS ONE 6(5): e19240 Signalogs: orthology-based identification of novel signaling pathway components in three metazoans. [FBrf0213696] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...