Open Close
General Information
Symbol
Dmel\SERCA
Species
D. melanogaster
Name
Sarco/endoplasmic reticulum Ca(2+)-ATPase
Annotation Symbol
CG3725
Feature Type
FlyBase ID
FBgn0263006
Gene Model Status
Stock Availability
Enzyme Name (EC)
P-type H(+)-exporting transporter (7.1.2.1)
P-type Ca(2+) transporter (7.2.2.10)
Gene Snapshot
Sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA) encodes an endoplasmic reticulum (ER) calcium pump with roles in ER calcium homeostasis and lipid storage. [Date last reviewed: 2019-09-26]
Also Known As
Ca-P60A, dSERCA, Calcium ATPase at 60A, Kum, CaP60A
Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:23,925,077..23,935,884 [-]
Recombination map
2-105
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. (P22700)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + H(2)O + H(+)(In) = ADP + phosphate + H(+)(Out) (7.1.2.1)
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2) (7.2.2.10)
Summaries
Gene Group (FlyBase)
HALOACID DEHALOGENASES -
The Haloacid Dehalogenase (HAD) family members are hydrolases characterized by a conserved α/β-domain Rossmann fold-like domain and three additional domains: squiggle, flap and cap domains, that contribute to substrate specificity. HADs are aspartate-nucleophile hydrolases, using a aspartate residue for nucleophilic attack. This family includes phosphoesterases, ATPases, phosphonatases, dehalogenases and sugar phosphomutases. (Adapted from FBrf0228712).
SARCO(ENDO)PLASMIC RETICULUM CALCIUM TRANSPORTING P-ATPASES -
Sarco(endo)plasmic reticulum Ca[2+] transporting ATPases (SERCA) are type IIA P-ATPases that utilize the hydrolysis of ATP to drive the transmembrane transport of Ca[2+] from the cytoplasm to the lumen of organelle. (Adapted from FBrf0224947).
Protein Function (UniProtKB)
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium.
(UniProt, P22700)
Gene Model and Products
Number of Transcripts
9
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\SERCA or the JBrowse view of Dmel\SERCA for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.52
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0072215
3890
1002
FBtr0072213
3385
1020
FBtr0072217
3395
1020
FBtr0072214
3259
1020
FBtr0072212
3310
1020
FBtr0072218
3839
1020
FBtr0072211
3269
1020
FBtr0072216
5028
1020
FBtr0343281
6306
999
Additional Transcript Data and Comments
Reported size (kB)
4.5 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0072124
109.6
1002
4.99
FBpp0072122
111.7
1020
5.06
FBpp0072126
111.7
1020
5.06
FBpp0072123
111.7
1020
5.06
FBpp0072121
111.7
1020
5.06
FBpp0072127
111.7
1020
5.06
FBpp0072120
111.7
1020
5.06
FBpp0072125
111.7
1020
5.06
FBpp0309946
109.3
999
5.10
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)
1002 (aa); 110 (kD)
Comments
External Data
Subunit Structure (UniProtKB)
Interacts with SclA and SclB.
(UniProt, P22700)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\SERCA using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (26 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from physical interaction with UniProtKB:Q9VWB9
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR030332
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000643382
(assigned by GO_Central )
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN001943383
(assigned by GO_Central )
Biological Process (16 terms)
Terms Based on Experimental Evidence (15 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000643382
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000643382
(assigned by GO_Central )
Cellular Component (6 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with UniProtKB:Q93084
(assigned by UniProt )
inferred from electronic annotation with InterPro:IPR001757, InterPro:IPR005782
(assigned by InterPro )
inferred from sequence or structural similarity with UniProtKB:Q93084
inferred from biological aspect of ancestor with PANTHER:PTN000643383
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
In situ hybridization to sections of adult flies shows that Ca-P60A transcripts are detected in each tissue tested, generally at a low level. High transcript levels are observed in the central nervous system and in muscles. Strong expression is apparent in the visual system, in ganglion cells around the ocelli, and in the antennal center. It is also apparent in the muscles of the proboscis, the cibarial pump, the lateral pharyngeal muscles, and the salivary muscles. High expression is also seen in some muscles of the mesothorax and in the jump muscles. Finally, elevated levels are observed in oocytes.
Ca-P60A transcripts are present in adult-males and females. None are detected in embryos, larvae, or pupae.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\SERCA in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 42 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 23 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of SERCA
Transgenic constructs containing regulatory region of SERCA
Deletions and Duplications ( 12 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
12 of 15
No
Yes
10 of 15
No
Yes
3 of 15
No
No
2 of 15
No
No
Rattus norvegicus (Norway rat) (6)
12 of 13
Yes
Yes
7 of 13
No
Yes
6 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (5)
10 of 12
Yes
Yes
6 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (7)
14 of 15
Yes
Yes
14 of 15
Yes
Yes
11 of 15
No
Yes
11 of 15
No
Yes
8 of 15
No
Yes
7 of 15
No
Yes
3 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
15 of 15
Yes
Yes
3 of 15
No
No
Arabidopsis thaliana (thale-cress) (5)
9 of 9
Yes
Yes
3 of 9
No
Yes
3 of 9
No
Yes
3 of 9
No
Yes
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (4)
4 of 15
Yes
No
3 of 15
No
No
3 of 15
No
Yes
3 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
2 of 12
Yes
Yes
2 of 12
Yes
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091901LU )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500X3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00WR )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00V6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01LE )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (5)
5 of 10
2 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 2 )
Allele
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Interacts with SclA and SclB.
(UniProt, P22700 )
Linkouts
DroID - A comprehensive database of gene and protein interactions.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Gene Group - Pathway Membership (FlyBase)
External Data
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-105
Cytogenetic map
Sequence location
2R:23,925,077..23,935,884 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
60A11-60A12
Left limit from in situ hybridisation (FBrf0157097) Right limit from in situ hybridisation (FBrf0157097)
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
60A11-60A12
60A8-60A11
(determined by in situ hybridisation)
60A-60A
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (27)
Genomic Clones (20)
cDNA Clones (521)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: SERCA Ca-P60A
    Source for database merge of
    Source for merge of: Ca-P60A CG3725
    Source for merge of: Ca-P60A Kum
    Source for merge of: Ca-P60A l(2)SH0211
    Additional comments
    Changed symbol from 'Ca-P60A' to 'SERCA' to reflect preferred usage in the literature.
    Other Comments
    Treatment of S2 cells with dsRNA made from templates generated with primers directed against this gene significantly increases the resting Ca2+ concentration, reduces the store release transient upon addition of thapsigargin, and strongly suppresses Ca2+ influx upon readdition of external Ca2+.
    One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.
    1 allele of l(2)SH0211 recovered in a P-insertion screen.
    Area matching Drosophila organellar-type Ca-ATPase gene, Acc. No. X84681.
    Flies carrying Ca-P60A mutations paralyse within 3 minutes at 40oC and remain immobile up to two days on shifting back to the permissive temperature.
    Ca-P60A is essential for cell viability and tissue morphogenesis during development. Ca-P60A function is required for intracellular trafficking of the N receptor.
    Ca-P60A show neither a maternal nor a zygotic neurogenic phenotype.
    Analysis of the conserved core sequence in 159 P-type ATPases from the 3 domains of life (Archaea, Bacteria and Eukarya) establishes 5 major groups of P-type ATPases.
    There is a single Ca-P60A gene in the Drosophila genome.
    Origin and Etymology
    Discoverer
    Etymology
    The gene is named "Kumbhakarna" based on the mutant phenotype; when restored to the permissive temperature, "Kumbhakarna" mutants show prolonged paralysis of 6-48 hours, the duration of paralysis depending on the duration of prior exposure to the restrictive temperature. "Kumbhakarna" is a mythological hero who slept for 6 months of the year.
    Identification
    External Crossreferences and Linkouts ( 83 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Linkouts
    ApoDroso - Functional genomic database for photoreceptor development, survival and function
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (35)
    Reported As
    Symbol Synonym
    A4UZU0_DROME
    Ca-p
    l(2)SH0211
    Name Synonyms
    Sarco/endoplasmic reticulum Ca(2+)-ATPase
    calcium ATPase
    calcium ATPase at 60A
    endoplasmic reticulum-specific calcium-transporting ATPase
    lethal (2) SH0211
    organellar-type Ca-ATPase
    sarco endoplasmic reticulum calcium-ATPase
    sarco/endoplasmic reticulum-type Ca-2+-ATPase
    sarcoplasmic endoplasmic reticulum calcium ATPase type 2
    Secondary FlyBase IDs
    • FBgn0004551
    • FBgn0004119
    • FBgn0004241
    • FBgn0022016
    • FBgn0022044
    • FBgn0026413
    • FBgn0034932
    • FBgn0065896
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (189)