FB2021_06, released December 14, 2021

Gene: Dmel\Klp54D

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General Information
Symbol
Dmel\Klp54D
Species
D. melanogaster
Name
Kinesin-like protein at 54D
Annotation Symbol
CG43323
Feature Type
protein_coding_gene
FlyBase ID
FBgn0263076
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

DmKlp54D

Key Links
Genomic Location
Cytogenetic map
54C5-54C8
Sequence location
Recombination map
2-83
RefSeq locus
NT_033778 REGION:17557031..17563406
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables ATP hydrolysis activity
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
enables microtubule binding
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
enables microtubule motor activity
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in microtubule-based movement
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of kinesin complex
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
is_active_in microtubule
inferred from biological aspect of ancestor with PANTHER:PTN000648413
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
KINESINS -
Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
5

Please see the JBrowse view of Dmel\Klp54D for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 6.04

Unconventional translation start (GUG) postulated; FlyBase analysis.

Sequence Ontology: Class of Gene
gene_with_non_canonical_start_codon
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Klp54D-RA
FBtr0306905
NM_001259469
2278
628
Klp54D-RB
FBtr0306906
NM_080144
2359
724
Klp54D-RC
FBtr0340221
NM_001299610
2238
636
Klp54D-RD
FBtr0392907
NM_001316425
2421
727
Klp54D-RE
FBtr0392908
NM_001316426
2421
760
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Klp54D-PA
FBpp0297759
68.8
628
9.46
NP_001246398
AFH08151
Klp54D-PB
FBpp0297760
79.6
724
9.44
NP_524883
AAF57836
Klp54D-PC
FBpp0309196
69.8
636
9.34
NP_001286539
AHN56335
Klp54D-PD
FBpp0352249
80.0
727
9.44
NP_001303354
ALI30183
Klp54D-PE
FBpp0352250
83.5
760
9.43
NP_001303355
ALI30184
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Klp54D using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Klp54D in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Klp54D
Transgenic constructs containing regulatory region of Klp54D
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable | recessive
Sterility
Allele
fertile | recessive
Phenotype manifest in
Allele
meiotic spindle | oogenesis
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (10)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\KIF12
14 of 15
Yes
Yes
Hsap\KIF3A
1 of 15
No
No
1  
Hsap\KIF3B
1 of 15
No
No
Hsap\KIF3C
1 of 15
No
No
Hsap\KIF6
1 of 15
No
Yes
Hsap\KIF9
1 of 15
No
Yes
Hsap\KIF15
1 of 15
No
Yes
Hsap\KIF20A
1 of 15
No
No
Hsap\KIF26A
1 of 15
No
No
Hsap\KIF26B
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Kif12
13 of 15
Yes
Yes
Mmus\Kif3a
1 of 15
No
No
1  
Mmus\Kif3b
1 of 15
No
No
Mmus\Kif3c
1 of 15
No
No
Mmus\Kif6
1 of 15
No
Yes
Mmus\Kif9
1 of 15
No
Yes
Mmus\Kif15
1 of 15
No
Yes
Mmus\Kif26a
1 of 15
No
No
Mmus\Kif26b
1 of 15
No
No
Rattus norvegicus (Norway rat) (8)
Rnor\Kif12
12 of 13
Yes
Yes
Rnor\Kif3a
1 of 13
No
No
Rnor\Kif3b
1 of 13
No
No
Rnor\Kif3c
1 of 13
No
No
Rnor\Kif6
1 of 13
No
Yes
Rnor\Kif9
1 of 13
No
Yes
Rnor\Kif26a
1 of 13
No
No
Rnor\Kif26b
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (6)
Xtro\kif3a
1 of 12
Yes
No
Xtro\kif3b
1 of 12
Yes
No
Xtro\kif3c
1 of 12
Yes
No
Xtro\kif9
1 of 12
Yes
Yes
Xtro\kif15
1 of 12
Yes
Yes
Xtro\kif26a
1 of 12
Yes
No
Danio rerio (Zebrafish) (11)
Drer\kif3a
1 of 15
Yes
No
Drer\kif3cb
1 of 15
Yes
No
Drer\kif3c
1 of 15
Yes
No
Drer\kif9
1 of 15
Yes
Yes
Drer\kif15
1 of 15
Yes
Yes
Drer\kif17
1 of 15
Yes
No
Drer\kif26aa
1 of 15
Yes
No
Drer\kif26ab
1 of 15
Yes
No
Drer\kif26ba
1 of 15
Yes
No
Drer\kif26bb
1 of 15
Yes
No
Drer\si:ch211-63b16.4
1 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\klp-10
1 of 15
Yes
Yes
Cele\klp-11
1 of 15
Yes
No
Cele\klp-18
1 of 15
Yes
No
Cele\klp-20
1 of 15
Yes
No
Cele\osm-3
1 of 15
Yes
No
Cele\vab-8
1 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (2)
Atha\AT4G26660
1 of 9
Yes
Yes
Atha\AT5G55520
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919040U )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00003584
Drosophila simulans
Dsim\GD11290
Drosophila sechellia
Dsec\GM21800
Drosophila erecta
Dere\GG21798
Drosophila yakuba
Dyak\GE11873
Drosophila ananassae
Dana\GF11642
Drosophila pseudoobscura pseudoobscura
Dpse\GA24738
Drosophila persimilis
Dper\GL11466
Drosophila willistoni
Dwil\GK21901
Drosophila virilis
Dvir\GJ21429
Drosophila mojavensis
Dmoj\GI18360
Drosophila grimshawi
Dgri\GH20922
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501UO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17023844
Glossina morsitans
Tsetse fly
Gmor\GMOY001368
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_07120
Mayetiola destructor
Hessian fly
Mdes\Mdes011623
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL000773
Anopheles darlingi
American malaria mosquito
Adar\ADAC008379
Anopheles gambiae
Malaria mosquito
Agam\AGAP011874
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001422
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ016560
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0HP1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA009884
Danaus plexippus
Monarch butterfly
Dple\DPOGS200831-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL012629
Apis florea
Little honeybee
Aflo\2178234468
Apis mellifera
Western honey bee
Amel\kinesin-12
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP13937
Bombus terrestris
Buff-tailed bumblebee
Bter\15215341
Linepithema humile
Argentine ant
Lhum\LH12263
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000bdc
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08372
Tribolium castaneum
Red flour beetle
Tcas\TC031474
Pediculus humanus
Human body louse
Phum\PHUM354900
Rhodnius prolixus
Kissing bug
Rpro\RPRC002395
Cimex lectularius
Bed bug
Clec\CLEC001808
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI066810
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_17937
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0HRO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR005873
Ixodes scapularis
Black-legged tick
Isca\ISCW020188
Stegodyphus mimosarum
African social velvet spider
Smim\KFM61511.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur07g06350
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_314281
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0SNP )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Kif12L
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Kif12
Ciona intestinalis
Vase tunicate
Cint\LOC100179715
Gallus gallus
Domestic chicken
Ggal\KIF12
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (27)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Kif3C
3 of 10
Klp64D
3 of 10
Klp68D
3 of 10
cana
2 of 10
CG14535
2 of 10
CG32318
2 of 10
cmet
2 of 10
cos
2 of 10
Khc-73
2 of 10
Khc
2 of 10
Kif19A
2 of 10
Klp3A
2 of 10
Klp10A
2 of 10
Klp31E
2 of 10
Klp59C
2 of 10
Klp59D
2 of 10
Klp61F
2 of 10
Klp67A
2 of 10
Klp98A
2 of 10
ncd
2 of 10
neb
2 of 10
nod
2 of 10
pav
2 of 10
sub
2 of 10
unc-104
2 of 10
CG5004
1 of 10
Gγ1
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    KIF12; kinesin family member 12
    14 of 15
    611278
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Interactions Browser
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Klp61F
        suppressible
        Klp54D
        (Radford et al., 2017)
        External Data
        Linkouts
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2R
        Recombination map
        2-83
        Cytogenetic map
        54C5-54C8
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        54C5-54C8
        Limits computationally determined from genome sequence between P{EP}MESR4EP386&P{EP}POSHEP1206 and P{PZ}l(2)1050510505
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        2-83

        (FlyBase, 2016)

        2-86.2

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (7)
        Genomic Clones (26)
        cDNA Clones (2)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
          Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of
            Source for database merge of

            Source for merge of: Klp54D CG15844

            (Misra, 2000.4.8)
            Additional comments

            Annotation CG15844 split into CG43323 (which corresponds to Klp54D) and CG43324 in release 5.36 of the genome annotation.

            (FlyBase Genome Annotators, 2011)
            Other Comments

            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

            (Kiger et al., 2003)

            Klp54D has been cloned, partially sequenced and its expression pattern analysed.

            (Stewart et al., 1991)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 40 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Synonyms and Secondary IDs (12)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (42)