FlyBase Gene Report: Dmel\Klp54D

General Information

Symbol

Dmel\Klp54D

Species

D. melanogaster

Name

Kinesin-like protein at 54D

Annotation Symbol

CG43323

Feature Type

protein_coding_gene

FlyBase ID

FBgn0263076

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

DmKlp54D

Key Links

Genomic Location

Cytogenetic map

54C5-54C8

Sequence location

2R:17,557,031..17,563,406 [+]

Recombination map

2-83

RefSeq locus

NT_033778 REGION:17557031..17563406

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

enables microtubule binding

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001752

(InterPro Project Members, 2004-)

enables microtubule motor activity

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821, InterPro:IPR027640

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in microtubule-based movement

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821, InterPro:IPR027640

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

part_of kinesin complex

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

is_active_in microtubule

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

KINESINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Group (FlyBase)

KINESINS -: Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Klp54D for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A126GUN8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 6.04

Unconventional translation start (GUG) postulated; FlyBase analysis.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Klp54D-RA

FBtr0306905

NM_001259469

2278

628

Klp54D-RB

FBtr0306906

NM_080144

2359

724

Klp54D-RC

FBtr0340221

NM_001299610

2238

636

Klp54D-RD

FBtr0392907

NM_001316425

2421

727

Klp54D-RE

FBtr0392908

NM_001316426

2421

760

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Klp54D-PA

FBpp0297759

68.8

628

9.46

FBpp0297760

79.6

724

9.44

FBpp0309196

69.8

636

9.34

FBpp0352249

80.0

727

9.44

FBpp0352250

83.5

760

9.43

A0A126GUN8

NP_001303355

ALI30184

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Klp54D using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Klp54D_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.39

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: PBac{768.FSVS-0}Klp54D^CPTI003686

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

adult gnathal ganglion

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Klp54D in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Klp54D

View exonic expression by tissue for Dmel\Klp54D

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

47216/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263076

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263076

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Klp54D

Transgenic constructs containing regulatory region of Klp54D

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable | recessive

Klp54D³

Sterility

Allele

fertile | recessive

Klp54D³

Phenotype manifest in

Allele

meiotic spindle | oogenesis

Klp54D³

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (35)

Hsap\KIF12

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\KIF15

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (15)

Rnor\Kif12

13 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (18)

Mmus\Kif12

12 of 14

Yes

Mmus\Kif3a

2 of 14

Mmus\Kif3b

2 of 14

Mmus\Kif6

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (15)

Xtro\bbs2

1 of 13

Yes

Xtro\kif5c

1 of 13

Yes

Xtro\kif9

1 of 13

Yes

Xtro\kif11

1 of 13

Yes

Xtro\kif13a

1 of 13

Yes

Xtro\kif13b

1 of 13

Yes

Xtro\kif14

1 of 13

Yes

Xtro\kif18a

1 of 13

Yes

Xtro\kif18b

1 of 13

Yes

Xtro\kif20a

1 of 13

Yes

Xtro\kif20b

1 of 13

Yes

Xtro\kif23

1 of 13

Yes

Xtro\kif27

1 of 13

Yes

Xtro\kifc2

1 of 13

Yes

Xtro\LOC100490157

1 of 13

Yes

Danio rerio (Zebrafish) (21)

Drer\kif3a

2 of 14

Yes

Drer\kif3cb

2 of 14

Yes

Drer\kif3c

2 of 14

Yes

Drer\kif15

2 of 14

Yes

Drer\kif17

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (13)

Cele\klp-20

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (18)

Agam\AgaP_AGAP011874

12 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (44)

Atha\AT3G20150

2 of 13

Yes

Atha\AT3G44050

2 of 13

Yes

Atha\KINESIN-12B

2 of 13

Yes

Atha\PAKRP1

2 of 13

Yes

Atha\POK2

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\KIP1

1 of 13

Yes

Scer\KIP3

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\cut7

1 of 12

Yes

Spom\klp3

1 of 12

Yes

Spom\klp5

1 of 12

Yes

Spom\klp6

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Klp54D. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (27)

4 of 13

4 of 13

4 of 13

3 of 13

Khc

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

pav

3 of 13

sub

3 of 13

CG14535

2 of 13

cmet

2 of 13

cos

2 of 13

Kif19A

2 of 13

Klp3A

2 of 13

Klp61F

2 of 13

ncd

2 of 13

neb

2 of 13

nod

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KIF12; kinesin family member 12

13 of 14

611278

CHOLESTASIS, PROGRESSIVE FAMILIAL INTRAHEPATIC, 8; PFIC8

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Klp61F

suppressible

Klp54D

(Radford et al., 2017)

External Data

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_non_canonical_start_codon

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-83

Cytogenetic map

54C5-54C8

Sequence location

2R:17,557,031..17,563,406 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

54C5-54C8

Limits computationally determined from genome sequence between P{EP}MESR4^EP386&P{EP}POSH^EP1206 and P{PZ}l(2)10505¹⁰⁵⁰⁵

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-83

(FlyBase, 2016)

2-86.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Genomic Clones (26)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (2)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP14187

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0263076

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

DMG4-chr2R.24.005.pred.a

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Klp54D has been cloned, partially sequenced and its expression pattern analysed.

(Stewart et al., 1991)

Relationship to Other Genes

Source for database merge of

Source for merge of: Klp54D CG15844

(Misra, 2000.4.8)

Additional comments

Annotation CG15844 split into CG43323 (which corresponds to Klp54D) and CG43324 in release 5.36 of the genome annotation.

(FlyBase Genome Annotators, 2011)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

CG15844

(Keleman et al., 2009.8.5, Miki et al., 2001, Goldstein and Gunawardena, 2000, Greene, 2000.4.11)

CG43323

(FlyBase Genome Annotators, 2011, Ni et al., 2011.7.19, Perkins et al., 2009)

DmKlp54D

(Hirokawa, 2012, Dagenbach and Endow, 2004)

KIF 12

(Miki et al., 2001)

KIF12

(Hirokawa, 2012.11.20)

KLP1

(Stewart et al., 1991)

KLP54D

(Miki et al., 2005)

Klp1

Klp54D

(Deng et al., 2021, Feng et al., 2021, Witte et al., 2020, Hughes et al., 2018, Radford et al., 2017, Transgenic RNAi Project members, 2017-, Hudry et al., 2016, Matthews et al., 2015, Liang et al., 2014, Hirokawa, 2012.11.20, Long et al., 2008, Stark et al., 2007)

Name Synonyms

Kinesin-like protein 54D

(Long et al., 2008)

Kinesin-like protein at 54D

(Hirokawa, 2012.11.20)

Kinesin4A

(Kollmar, 2015.9.15)

Secondary FlyBase IDs

FBgn0004377
FBgn0034241
FBan0015844
FBgn0263028

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

47216

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A126GUN8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A126GUN8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

47216/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263076

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263076

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG43323

MARRVEL_MODEL - MARRVEL (model organism gene)

47216

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0263076

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Klp54D_1

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0263076

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031474

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (43)

The following external sites may use different assemblies or annotations than FlyBase.

-0.39

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