General Information
Symbol
Dmel\psq
Species
D. melanogaster
Name
pipsqueak
Annotation Symbol
CG2368
Feature Type
FlyBase ID
FBgn0263102
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
eyeful, psk, zep, BTB-V, pip
Genomic Location
Cytogenetic map
Sequence location
2R:10,557,888..10,617,280 [+]
Recombination map
2-62
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Protein Family (UniProt, Sequence Similarities)
-
Molecular Function (see GO section for details)
Summaries
Gene Group Membership
POLYCOMB GROUP RECRUITERS/DNA-BINDING PROTEINS -
The Polycomb group (PcG) proteins are epigenetic regulators, best characterized for the repression of Hox gene expression. In Drosophila, PcG proteins repress their target genes by binding to specific DNA elements called Polycomb Response Elements (PREs). PcG DNA binding proteins contribute to the recruitment of Polycomb complexes to PREs. (Adapted from FBrf0228921).
PIPSQUEAK TRANSCRIPTION FACTORS -
Pipsqueak transcription factors are sequence-specific DNA binding proteins that regulate transcription. These family members are characterized by a heix-turn-helix DNA-binding domain called Psq domain, which consists of four tandem repeats of a conserved 50-amino acid sequence, the Psq motif. (Adapted from FBrf0148954 and FBrf0105267).
Gene Model and Products
Number of Transcripts
12
Number of Unique Polypeptides
6

Please see the GBrowse view of Dmel\psq or the JBrowse view of Dmel\psq for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.44
Stop-codon suppression (UGA) postulated; FBrf0216884
gene_with_stop_codon_read_through ; SO:0000697
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
Gene model reviewed during 6.02
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0088276
5313
1046
FBtr0088275
5367
1064
FBtr0088282
5210
1064
FBtr0088278
4725
645
FBtr0088277
4464
645
FBtr0088279
4780
645
FBtr0088280
4513
645
FBtr0088281
4550
645
FBtr0100606
4889
639
FBtr0100607
4640
639
FBtr0330607
6336
1123
FBtr0346958
6336
1043
Additional Transcript Data and Comments
Reported size (kB)
6.0, 5.1, 4.4 (northern blot)
5.7, 5.0, >7.0 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0087369
113.0
1046
6.75
FBpp0087368
115.0
1064
6.73
FBpp0089330
115.0
1064
6.73
FBpp0087371
70.3
645
6.79
FBpp0087370
70.3
645
6.79
FBpp0087372
70.3
645
6.79
FBpp0087373
70.3
645
6.79
FBpp0087374
70.3
645
6.79
FBpp0100066
69.7
639
6.79
FBpp0100067
69.7
639
6.79
FBpp0303457
119.8
1123
6.87
FBpp0312422
112.6
1043
6.75
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

645 aa isoforms: psq-PD, psq-PE, psq-PF, psq-PG, psq-PH
1064 aa isoforms: psq-PB, psq-PC
639 aa isoforms: psq-PJ, psq-PK
Additional Polypeptide Data and Comments
Reported size (kDa)
1065 (aa); 150 (kD observed)
1085, 666, 660, 535 (aa)
Comments
Downstream from the BTB (or POZ) domain, the psq+P1065 protein contains 34 alternating histidine residues, (HX)n,which may act as an additional protein-protein interaction domain. The psq protein also contains a 52-55 amino acid motif tandemly repated four times at the C terminus, which the authors call "the psq motif". The function of the evolutionarily conserved psq motif is unknown. The putative product of the psq+R4.4 transcript lacks both the BTB and (HX)n motifs, but still posesses the psq motifs.
The BTB-domain is predicted for the products of the class A transcripts (psq+R7.0A1 and psq+R7.0A2) only, psq+P1085 and psq+P535.
one of several products generated by alternative splicing
External Data
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\psq using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (14 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
traceable author statement
Biological Process (8 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
traceable author statement
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN001135010
(assigned by GO_Central )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001135010
(assigned by GO_Central )
traceable author statement
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states 0-2 hr AEL

Additional Descriptive Data
psq transcripts are first distributed uniformly in the embryo. At stage 5 transcript accumulation is observed dorsally between 20 and 70% egg length. In addition to a low level uniform expression, high levels of psq transcripts are found through stage 7/8 in the dorso-anterior domain and in the amnioserosa (detected with an intronic probe). In later stages, expression is detected in the mandibular, maxillary, and labial lobes.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
psq protein localizes to the nucleus.
The psq protein localizes to the nuclei of somatic and germ cells during oogenesis.
psq protein is detected in larvae in ommatidial precursor cells with the highest levels in R3 and R4. In wing discs, a cross-like pattern of psq protein is observed in the wing blade primordium above uniform low-level expression.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\psq in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 86 )
For All Classical and Insertion Alleles Show
 
Allele of psq
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    --
    Other relevant insertions
    insertion of mobile activating element
    miscellaneous insertions
    Transgenic Constructs ( 10 )
    Deletions and Duplications ( 13 )
    Summary of Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Sterility
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    adult head & macrochaeta
    adult thorax & macrochaeta
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (1)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    1 of 15
    Yes
    No
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (3)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    1 of 15
    Yes
    Yes
    1 of 15
    Yes
    No
    1 of 15
    Yes
    No
    Rattus norvegicus (Norway rat) (2)
    1 of 13
    Yes
    No
    1 of 13
    Yes
    No
    Xenopus tropicalis (Western clawed frog) (0)
    No orthologs reported.
    Danio rerio (Zebrafish) (1)
    1 of 15
    Yes
    No
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No orthologs reported.
    Arabidopsis thaliana (thale-cress) (0)
    No orthologs reported.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No orthologs reported.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No orthologs reported.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919059L )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150291 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Glossina morsitans
    Tsetse fly
    Lucilia cuprina
    Australian sheep blowfly
    Mayetiola destructor
    Hessian fly
    Aedes aegypti
    Yellow fever mosquito
    Anopheles darlingi
    American malaria mosquito
    Anopheles gambiae
    Malaria mosquito
    Culex quinquefasciatus
    Southern house mosquito
    Culex quinquefasciatus
    Southern house mosquito
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0DEQ )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Danaus plexippus
    Monarch butterfly
    Heliconius melpomene
    Postman butterfly
    Apis florea
    Little honeybee
    Apis mellifera
    Western honey bee
    Apis mellifera
    Western honey bee
    Bombus impatiens
    Common eastern bumble bee
    Bombus terrestris
    Buff-tailed bumblebee
    Linepithema humile
    Argentine ant
    Linepithema humile
    Argentine ant
    Megachile rotundata
    Alfalfa leafcutting bee
    Nasonia vitripennis
    Parasitic wasp
    Dendroctonus ponderosae
    Mountain pine beetle
    Tribolium castaneum
    Red flour beetle
    Pediculus humanus
    Human body louse
    Rhodnius prolixus
    Kissing bug
    Cimex lectularius
    Bed bug
    Cimex lectularius
    Bed bug
    Acyrthosiphon pisum
    Pea aphid
    Zootermopsis nevadensis
    Nevada dampwood termite
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0DCI )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Daphnia pulex
    Water flea
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0LAU )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Ciona intestinalis
    Vase tunicate
    Human Disease Model Data
    FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 2 )
    Allele
    Disease
    Evidence
    References
    model of  cancer
    in combination with EgfrUAS.cBa
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-62
    Cytogenetic map
    Sequence location
    2R:10,557,888..10,617,280 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    47A13-47B1
    Limits computationally determined from genome sequence between P{PZ}lola00349&P{EP}lolaEP952 and P{lacW}l(2)k00909k00909&P{EP}EP2619EP2619
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    47B1-47B4
    47A10-47A12
    47B-47B
    (determined by in situ hybridisation)
    47A10-47A12
    (determined by in situ hybridisation)
    47B1-47B4
    (determined by in situ hybridisation)
    47A-47A
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (43)
    Genomic Clones (26)
    cDNA Clones (150)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: psq CG2368
    Source for database merge of
    Source for merge of: psq zep
    Source for merge of: psq BtbV
    Source for merge of: psq rum
    Additional comments
    Source for merge of psq BtbV was sequence comparison ( date:000202 ).
    Other Comments
    RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
    Genetic interaction data suggest that psq may have a role in control of homeotic gene activity.
    Identification: as a suppressor of the small eye phenotype caused by overexpression of fng in the eye.
    'eyeful' mutant interacts genetically with N and fng though it is not yet clear whether 'eyeful' corresponds to lola or psq.
    psq has been cloned and molecularly characterised. psq is a complex gene encoding multiple differentially spliced transcripts and several protein isoforms.
    P{PZ} insertion within an intron causes an aberrant splicing and transcription termination event due to l(3)87Df sequences flanking the HindIII ry fragment within the P{PZ} element. The aberrant splicing event is entirely responsible for the mutant phenotype.
    High level expression of psq is svp-dependent and thus psq might be acting downstream of svp for the development of photoreceptor cells 3 and 4. psq is also required for the development of other adult structures.
    The psq gene is a member of the posterior group of genes and is required for early stages of oogenesis, acting at least partly through vas.
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 128 )
    Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Linkouts
    ApoDroso - Functional genomic database for photoreceptor development, survival and function
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
    DroID - A comprehensive database of gene and protein interactions.
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    KEGG Genes - Molecular building blocks of life in the genomic space.
    Synonyms and Secondary IDs (22)
    Reported As
    Symbol Synonym
    BtbV
    Secondary FlyBase IDs
    • FBgn0069087
    • FBgn0004399
    • FBgn0012047
    • FBgn0045853
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (191)