FlyBase Gene Report: Dmel\5-HT1B

General Information

Symbol

Dmel\5-HT1B

Species

D. melanogaster

Name

5-hydroxytryptamine (serotonin) receptor 1B

Annotation Symbol

CG15113

Feature Type

protein_coding_gene

FlyBase ID

FBgn0263116

Gene Model Status

Current

Stock Availability

43 publicly available

Gene Summary

5-hydroxytryptamine (serotonin) receptor 1B (5-HT1B) encodes a 5-hydroxytryptamine (serotonin) GPCR that belongs to the Class A GPCR family. They bind and transmit the signal from the neurotransmitter 5-HT (serotonin). The family can be subdivided into several subclasses based on pharmacology, signal transduction and structure. There are 5 genes encoding serotonin receptors in the Drosophila melanogaster genome: 5-HT1A, 5-HT1B, 5-HT2A, 5-HT2B, and 5-HT7. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

5HT1B, 5HT-dro2B, d5-HT1B, 5-HT_1B, 5-HT1BDro

Key Links

Genomic Location

Cytogenetic map

56B1-56B1

Sequence location

2R:19,022,325..19,032,642 [-]

Recombination map

2-88

RefSeq locus

NT_033778 REGION:19022325..19032642

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables G protein-coupled serotonin receptor activity

inferred from direct assay

(Gasque et al., 2013)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables G protein-coupled amine receptor activity

inferred from sequence or structural similarity

(Radford et al., 2002)

enables G protein-coupled receptor activity

inferred from electronic annotation with InterPro:IPR000276

(InterPro Project Members, 2004-)

enables G protein-coupled serotonin receptor activity

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

traceable author statement

(Tierney, 2001)

inferred from biological aspect of ancestor with PANTHER:PTN000664111

(GO Reference Genome Project, 2011-)

enables Gi/o-coupled serotonin receptor activity

non-traceable author statement

(Tierney, 2001)

enables neurotransmitter receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000664111

(GO Reference Genome Project, 2011-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in adenylate cyclase-inhibiting serotonin receptor signaling pathway

inferred from direct assay

(Saudou et al., 1992)

involved_in entrainment of circadian clock

inferred from mutant phenotype

(Yuan et al., 2005)

involved_in phospholipase C-activating serotonin receptor signaling pathway

inferred from direct assay

(Saudou et al., 1992)

involved_in serotonin receptor signaling pathway

inferred from direct assay

(Gasque et al., 2013)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in adenylate cyclase-inhibiting serotonin receptor signaling pathway

traceable author statement

(Tierney, 2001)

inferred from biological aspect of ancestor with PANTHER:PTN002797960

(GO Reference Genome Project, 2011-)

involved_in chemical synaptic transmission

inferred from biological aspect of ancestor with PANTHER:PTN000664111

(GO Reference Genome Project, 2011-)

involved_in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger

inferred from biological aspect of ancestor with PANTHER:PTN000664111

(GO Reference Genome Project, 2011-)

involved_in G protein-coupled receptor signaling pathway

inferred from electronic annotation with InterPro:IPR000276

(InterPro Project Members, 2004-)

involved_in phospholipase C-activating serotonin receptor signaling pathway

traceable author statement

(Tierney, 2001)

involved_in serotonin receptor signaling pathway

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in dendrite

inferred from biological aspect of ancestor with PANTHER:PTN000664111

(GO Reference Genome Project, 2011-)

located_in membrane

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

inferred from electronic annotation with InterPro:IPR000276, InterPro:IPR017452

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000664111

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

5-HYDROXYTRYPTAMINE (SEROTONIN) GPCRs

Protein Family (UniProt)

Belongs to the G-protein coupled receptor 1 family. (P28286)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

5-HYDROXYTRYPTAMINE (SEROTONIN) GPCRs -: 5-hydroxytryptamine (serotonin) GPCRs belong to the Class A GPCR family. They bind and transmit the signal from the neurotransmitter 5-HT (serotonin). The family can be subdivided into seven subclasses: 5-HT1-7 based on pharmacology, signal transduction and structure. (Adapted from PMID:18571247).

Protein Function (UniProtKB)

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins which inhibit adenylate cyclase.

(UniProt, P28286)

Summary (Interactive Fly)

seven-pass transmembrane protein, 5-HT1A promotes baseline sleep, aggression, courtship and memory formation

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\5-HT1B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P28286)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Stop-codon suppression (UGA) postulated; FBrf0216884

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

5-HT1B-RA

FBtr0086599

NM_079065

2980

617

5-HT1B-RD

FBtr0330055

NM_001169730

3861

562

5-HT1B-RE

FBtr0330056

NM_001144236

4026

629

Additional Transcript Data and Comments

Reported size (kB)

4.9 (northern blot)

(Saudou et al., 1992)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

5-HT1B-PA

FBpp0085783

67.5

617

8.04

FBpp0303088

61.9

562

8.76

FBpp0303089

69.0

629

7.75

NP_001137708

ACL83162

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

645 (aa)

(Saudou et al., 1992)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\5-HT1B using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

5-HT1B_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.87

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

bipolar dendrite neuron

(Saudou et al., 1992)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

ventral midline of embryo

(Saudou et al., 1992)

Additional Descriptive Data

5-HT1B transcripts are detected throughout development with a peak in 16-24hr embryos. They are found in adult heads but not bodies. In embryos, expression starts in stage 16 and is restricted to the CNS. 5-HT1B transcripts are expressed in lateral rows of cells on each side of the midline and at a low level in midline cells.

(Saudou et al., 1992)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

hemocyte

(Qi et al., 2016)

adult head

(Qi et al., 2016)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{5-HT1B-GAL4.A}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

dopaminergic PPL1 neuron | subset

(Aso et al., 2012)

mushroom body vertical lobe arborizing neuron 1 | subset

(Aso et al., 2012)

mushroom body medial-vertical lobe arborizing neuron 1 | subset

(Aso et al., 2012)

Reporter: P{5-HT1B-GAL4.Y}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

neuron | subset

(Zhou et al., 2012)

third instar larval stage & adult stage

brain | restricted

(Luo et al., 2012)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\5-HT1B in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\5-HT1B

View exonic expression by tissue for Dmel\5-HT1B

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37191/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263116

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263116

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 18 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of 5-HT1B

Transgenic constructs containing regulatory region of 5-HT1B

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel6068

(Ryder, 2004.4.26)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

5-HT1B^GD13681

lethal | pupal stage, with Scer\GAL4^pnr-MD237

5-HT1B^GD13681

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

5-HT1B^GD617

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

5-HT1B^GD617

Other Phenotypes

Allele

abnormal behavior | adult stage

abnormal circadian rhythm, with Scer\GAL4^5-HT1B.PY

abnormal circadian rhythm, with Scer\GAL4^elav-C155

abnormal circadian rhythm, with Scer\GAL4^tim.PE

abnormal immune response | larval stage

5-HT1B^ΔIII-V

abnormal immune response | larval stage, with Scer\GAL4^GMR64A03

5-HT1B^RNAi.UAS.cUa

abnormal immune response | larval stage, with Scer\GAL4^GMR76E11

abnormal immune response | larval stage, with Scer\GAL4^Hand.Δ

abnormal locomotor behavior | adult stage

5-HT1B^MB05181

abnormal mating behavior, with Scer\GAL4^elav-C155

5-HT1B^RNAi.UAS

abnormal phototaxis | adult stage, with Scer\GAL4^nSyb.PS

Phenotype manifest in

Allele

embryonic/larval lymph gland | larval stage, with Scer\GAL4^Hand.Δ

extracellular matrix | larval stage | conditional, with Scer\GAL4^Hand.Δ

5-HT1B^RNAi.UAS.cUa

lamellocyte | decreased number, with Scer\GAL4^Hand.Δ

5-HT1B^RNAi.UAS.cUa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (140)

Hsap\HTR1A

9 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\AVPR1B

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\CCR2

1 of 14

Yes

Hsap\CCR8

1 of 14

Yes

Hsap\CCRL2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\CNR2

1 of 14

Yes

Hsap\CXCR1

1 of 14

Yes

Hsap\CXCR2

1 of 14

Yes

Hsap\CXCR4

1 of 14

Yes

Hsap\CXCR5

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\EDNRB

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\GPR3

1 of 14

Yes

Hsap\GPR4

1 of 14

Yes

Hsap\GPR6

1 of 14

Yes

Hsap\GPR12

1 of 14

Yes

Hsap\GPR17

1 of 14

Hsap\GPR19

1 of 14

Yes

Hsap\GPR21

1 of 14

Hsap\GPR45

1 of 14

Yes

Hsap\GPR50

1 of 14

Hsap\GPR52

1 of 14

Hsap\GPR63

1 of 14

Yes

Hsap\GPR101

1 of 14

Hsap\GPR161

1 of 14

Yes

Hsap\GPR176

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\LPAR2

1 of 14

Yes

Hsap\LPAR3

1 of 14

Yes

Hsap\LPAR6

1 of 14

Hsap\MC1R

1 of 14

Yes

Hsap\MC2R

1 of 14

Yes

Hsap\MC3R

1 of 14

Yes

Hsap\MC4R

1 of 14

Yes

Hsap\MC5R

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\OR4K5

1 of 14

Yes

Hsap\OR4L1

1 of 14

Yes

Hsap\P2RY1

1 of 14

Yes

Hsap\P2RY8

1 of 14

Yes

Hsap\PROKR1

1 of 14

Yes

Hsap\PROKR2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\S1PR2

1 of 14

Yes

Hsap\S1PR3

1 of 14

Yes

Hsap\S1PR4

1 of 14

Yes

Hsap\S1PR5

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (31)

Rnor\Htr1a

10 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Rnor\Htr5b

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (35)

Mmus\Htr1a

8 of 14

Yes

Mmus\Htr1b

3 of 14

Mmus\Htr1f

3 of 14

Mmus\Htr5a

3 of 14

Yes

Mmus\Htr1d

2 of 14

Mmus\Htr5b

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Drd5

1 of 14

Mmus\Gpr63

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (103)

Xtro\htr1a

3 of 13

Yes

Xtro\htr5a

3 of 13

Yes

Xtro\htr1b

2 of 13

Xtro\htr1e

2 of 13

Xtro\ackr4

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\avpr2

1 of 13

Yes

Xtro\bdkrb2

1 of 13

Xtro\brs3

1 of 13

Xtro\calhm4

1 of 13

Yes

Xtro\ccr3

1 of 13

Yes

Xtro\ccr9

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\f2rl3

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\gpr12

1 of 13

Yes

Xtro\gpr27

1 of 13

Yes

Xtro\gpr65

1 of 13

Yes

Xtro\gpr78

1 of 13

Yes

Xtro\gpr83

1 of 13

Xtro\gpr173

1 of 13

Yes

Xtro\gpr174

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100496229

1 of 13

Yes

Xtro\LOC100498398

1 of 13

Yes

Xtro\LOC101733812

1 of 13

Yes

Xtro\LOC101734771

1 of 13

Xtro\LOC105946838

1 of 13

Xtro\LOC116411706

1 of 13

Yes

Xtro\lpar1

1 of 13

Yes

Xtro\lpar3

1 of 13

Yes

Xtro\mc4r

1 of 13

Yes

Xtro\mc5r

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\opn5

1 of 13

Xtro\opn8

1 of 13

Xtro\opnpl [provisonal]

1 of 13

Xtro\oprk1

1 of 13

Xtro\p2ry4

1 of 13

Yes

Xtro\qrfprl2.2

1 of 13

Yes

Xtro\qrfpr

1 of 13

Yes

Xtro\rho

1 of 13

Yes

Xtro\rrh

1 of 13

Xtro\s1pr1

1 of 13

Yes

Xtro\s1pr5

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tmtops

1 of 13

Yes

Xtro\trhr2

1 of 13

Xtro\triqk

1 of 13

Yes

Xtro\XB982628

1 of 13

Xtro\XB996286

1 of 13

Xtro\XB5959819

1 of 13

Yes

Danio rerio (Zebrafish) (83)

Drer\htr1aa

10 of 14

Yes

Drer\htr1ab

9 of 14

Yes

Drer\htr5aa

3 of 14

Yes

Drer\htr5ab

3 of 14

Yes

Drer\htr1b

2 of 14

Drer\htr1d

2 of 14

Drer\htr1fb

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\htr1e

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC100334962

1 of 14

Drer\LOC101885642

1 of 14

Yes

Drer\or90a1

1 of 14

Yes

Drer\or90b1

1 of 14

Yes

Drer\or90b2

1 of 14

Yes

Drer\or90c1

1 of 14

Yes

Drer\or90d1

1 of 14

Yes

Drer\or90d2

1 of 14

Yes

Drer\or90d3

1 of 14

Yes

Drer\or90e2

1 of 14

Yes

Drer\or90h1

1 of 14

Yes

Drer\or90h3

1 of 14

Yes

Drer\or90h4

1 of 14

Yes

Drer\or90j1

1 of 14

Yes

Drer\or92a1

1 of 14

Yes

Drer\or92a2

1 of 14

Yes

Drer\or92a7

1 of 14

Yes

Drer\or93a1

1 of 14

Yes

Drer\or93a7

1 of 14

Yes

Drer\or137-5

1 of 14

Yes

Drer\or137-8

1 of 14

Yes

Drer\or137-9

1 of 14

Yes

Drer\parietopsin

1 of 14

Yes

Drer\taar12a

1 of 14

Drer\taar12f

1 of 14

Yes

Drer\taar13a

1 of 14

Drer\taar13d

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (43)

Cele\ser-4

10 of 14

Yes

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (55)

Agam\GPR5HT1A

7 of 12

Yes

Agam\GPR5HT1B

5 of 12

Yes

3 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:5-HT1B. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (52)

9 of 13

5 of 13

5 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Lkr

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Rh2

1 of 13

Rh3

1 of 13

Rh4

1 of 13

Rh5

1 of 13

Rh6

1 of 13

Rh7

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

anxiety modulators, fly wall-following model

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

5-HT1B^KK112342

model of generalized anxiety disorder

CEA

(Mohammad et al., 2016)

5-HT1B^KK115609

model of generalized anxiety disorder

CEA

(Mohammad et al., 2016)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

HTR1A; 5-hydroxytryptamine receptor 1A

model of vesicoureteral reflux

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HTR1A; 5-hydroxytryptamine receptor 1A

9 of 14

109760

PERIODIC FEVER, MENSTRUAL CYCLE-DEPENDENT

vesicoureteral reflux

HTR1B; 5-hydroxytryptamine receptor 1B

3 of 14

5-HYDROXYTRYPTAMINE RECEPTOR 1B; HTR1B

HTR1E; 5-hydroxytryptamine receptor 1E

3 of 14

182132

HTR1F; 5-hydroxytryptamine receptor 1F

3 of 14

182134

HTR5A; 5-hydroxytryptamine receptor 5A

3 of 14

601305

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

5-HT1B

suppressible

cry

(Yuan et al., 2005)

suppressible

(Liu et al., 2025)

suppressible

(Liu et al., 2025)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cry

suppressible

5-HT1B

(Yuan et al., 2005)

sgg

suppressible

5-HT1B

(Yuan et al., 2005)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0263116

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Neuroactive ligand-receptor interaction

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-88

Cytogenetic map

56B1-56B1

Sequence location

2R:19,022,325..19,032,642 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

56B1-56B1

Limits computationally determined from genome sequence between P{lacW}prod^k08810 and P{PZ}ena⁰²⁰²⁹&P{lacW}cora^k08713

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

56A-56B

(determined by in situ hybridisation)

(Saudou et al., 1992)

Experimentally Determined Recombination Data

Location

2-88

(FlyBase, 2016)

2-93.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (43)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

27632

w^*; P{UAS-5-HT1B.Y}2

27633

w^*; P{UAS-5-HT1B.Y}3

27636

w^*; P{5-HT1B-GAL4.Y}2

27637

w^*; P{5-HT1B-GAL4.Y}3

86276

TI{GAL4}5-HT1B^Gal4/CyO

Korea

12106

P{VT019028-GAL4}attP2

15719

P{VT019028-lexA}attP2

16101

P{GMR52F09-lexA}attP40

16803

P{VT019028-lexA::GADfl}attP40

v109929

v110128

v9558

More stocks available...

Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (3)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

IP20237

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0263116

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CG30126) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

5-HT1B receptor inhibits adenylate cyclase and activates phospholipase C. Expression starts in late embryos and is restricted to distinct populations in the CNS. 5-HT1A and 5-HT1B have a common chromosomal location and high sequence homology suggesting they are the result of a recent duplication event.

(Saudou et al., 1992)

One of several known Drosophila serotonin receptor encoding genes. Presumed to be a recent duplication of 5-HT1A gene.

Relationship to Other Genes

Source for database merge of

Source for merge of: 5-HT1B CG30126

(FlyBase Genome Annotators, 2011)

Additional comments

Annotation CG30126 merged with annotation CG15113 in release 5.36 of the genome annotation; CG30126 corresponds to 5' UTR of CG15113.

(FlyBase Genome Annotators, 2011)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (32)

Reported As

Symbol Synonym

5-HT

(Pan et al., 2020)

5-HT-dro2B

(Tierney, 2001)

5-HT1B

(Tawa and Notterman, 2025, Cheng et al., 2024, Mallick et al., 2024, Qu et al., 2024, Gowda et al., 2023, Li et al., 2023, Rosikon et al., 2023, Wu et al., 2023, Banu et al., 2022, Cao et al., 2022, Hultmark and Andó, 2022, Ma et al., 2022, Marcogliese et al., 2022, Schlichting et al., 2022, Liu et al., 2021, Muria et al., 2021, Scaplen and Petruccelli, 2021, Chvilicek et al., 2020, He et al., 2020, Kondo et al., 2020, Sun et al., 2020, Hegazi et al., 2019, Meltzer et al., 2019, Rao and Deng, 2019.10.23, Park et al., 2018, Crocker et al., 2016, Gene Disruption Project members, 2016-, Rohde et al., 2016, Sarov et al., 2016, Gene Disruption Project members, 2015-, Gnerer et al., 2015, Ashwal-Fluss et al., 2014, Gasque et al., 2013, Xie et al., 2013, FlyBase Genome Annotators, 2011, Jungreis et al., 2011, Riedl et al., 2007)

5-HT1BDro

(Curran and Chalasani, 2012, Johnson et al., 2009, Maroteaux, 1995.5.31)

5-HT1b

(Kaneko et al., 2017)

5-HT_1B

(Qi et al., 2016, Kahsai et al., 2012, Luo et al., 2012, Johnson et al., 2011)

5-HT_1BDRO

(Luo et al., 2012)

5-HT_1BDro

(Singh et al., 2013, Johnson et al., 2011)

5-HT_1BDro

(Tierney, 2001)

5-HT_dro2B

(Obosi et al., 1996)

5-HT^1BDro

(Johnson et al., 2009)

5HT-1B

(Ries et al., 2017)

5HT-R2B

5HT-dro2B

(Tierney, 2001, Boschert et al., 1991, Boschert et al., 1991, Buchner, 1991)

5HT-dro_2B

(Hen, 1993)

5HT1B

(Bossen et al., 2023, Banu et al., 2022, Schlichting et al., 2022, Roy et al., 2020, Angilletta et al., 2019, Lin et al., 2019, Tang et al., 2017, Lee et al., 2011, Rodriguez Moncalvo and Campos, 2009, Dierick and Greenspan, 2007)

5HT1B-R

(Nässel, 2021)

5HT1b

(Qian et al., 2017, Ro et al., 2016)

5HTR1B

(Metaxakis et al., 2023, Palermo et al., 2023)

5htdro2b

(Feng et al., 1996)

CG15113

(Mao et al., 2024, National Institute of Genetics Fly Stocks, 2022-, Kondo et al., 2020, Park et al., 2018, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Hauser et al., 2006, Bryson-Richardson et al., 2004)

CG30126

(Keleman et al., 2009.8.5, Riedl et al., 2007)

DRO2B

(Han et al., 1998)

Dm5-HT1B

(Qi et al., 2017)

Dm5-HT_1B

(Blenau et al., 2017)

Dm5HTdro2B

(Blenau and Baumann, 2001)

HT1B

(Liu et al., 2015)

d5-HT1B

(Mazzotta et al., 2020, Mohammad et al., 2016, Yuan et al., 2006, Yuan et al., 2005, Yuan et al., 2005)

Name Synonyms

5-hydroxytryptamine (serotonin) receptor 1B

Drosophila serotonin receptor 1B

(Yuan et al., 2005)

Serotonin receptor 1B

Serotonin receptor 2B

Secondary FlyBase IDs

FBgn0050126
FBgn0004572

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37191

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

Z11490

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P28286

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P28286

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JGM2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P28286

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37191/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263116

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263116

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG15113

MARRVEL_MODEL - MARRVEL (model organism gene)

37191

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0263116

DroID - A comprehensive database of gene and protein interactions.

FBgn0263116

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

5-HT1B_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0263116

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0263116

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Neuroactive ligand-receptor interaction

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (146)

The following external sites may use different assemblies or annotations than FlyBase.

-0.87

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