FlyBase Gene Report: Dmel\Acsl

(Zhang et al., 2011)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

(Fisher et al., 2012)

embryonic stage 9 -- 10

(Fisher et al., 2012)

embryonic stage 11 -- 12

(Fisher et al., 2012)

embryonic stage 13 -- 16

(Fisher et al., 2012)

Additional Descriptive Data

Acsl transcripts are deposited maternally in early embryos.

(Zhang et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

day 7 of adulthood -- day 49 of adulthood

(Zhang et al., 2011)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Acsl protein is ubiquitously distributed in early embryos.

(Zhang et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in axon

inferred from direct assay

(O'Sullivan et al., 2012)

located_in cytoplasm

inferred from direct assay

(Zhang et al., 2011, Zhang et al., 2009)

located_in endoplasmic reticulum

inferred from direct assay

(Huang et al., 2016, Zhang et al., 2009)

located_in peroxisome

inferred from direct assay

(Huang et al., 2016)

Expression Deduced from Reporters

Reporter: P{PZ}Acsl⁰⁵⁸⁴⁷

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval fat body

(Meister and Braun, 1995.10)

adult stage

third segment of antenna

(Vosshall, 1998.8.10)

adult fat body

(Vosshall, 1998.8.10)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Acsl in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Acsl

View exonic expression by tissue for Dmel\Acsl

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263120

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

46068/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263120

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0010609

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263120

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8732

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 37 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Acsl

Transgenic constructs containing regulatory region of Acsl

Aberrations (Deficiencies and Duplications) ( 12 )

Inferred from experimentation ( 12 )

Gene disrupted in

Df(2R)44CE

(Mohr and Boswell, 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)Exel7098

(Ryder, 2004.4.26)

Df(2R)H3E1

(Liu et al., 2011, Zhang et al., 2009, Mohr and Boswell, 2002, Dockendorff et al., 2000)

Df(2R)Np3

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Acsl^GD1638

lethal (with Acsl¹)

Acsl⁸

lethal (with Acsl⁸)

lethal (with Acsl⁰⁵⁸⁴⁷)

Acsl⁸

lethal | recessive

lethal - all die before end of first instar larval stage | recessive

Acsl^KO

lethal - all die before end of larval stage | recessive

Acsl⁰⁵⁸⁴⁷

lethal - all die before end of P-stage | recessive

Acsl⁰⁵⁸⁴⁷

lethal - all die during embryonic stage

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Acsl^GD1638

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Acsl^GD1638

viable, with Scer\GAL4^pnr-MD237

Acsl^GD1638

viable, with Scer\GAL4^tin.CΔ4

Acsl^GD1638

Other Phenotypes

Allele

abnormal cell adhesion | oogenesis, with Scer\GAL4^slbo.2.6

Acsl^HMS02307

abnormal mitotic cell cycle | recessive

Acsl⁰⁵⁸⁴⁷

abnormal neuroanatomy | larval stage

Acsl^GD1638

abnormal neuroanatomy | larval stage (with Acsl⁸)

Acsl⁰⁵⁸⁴⁷

abnormal neuroanatomy | larval stage (with Acsl⁰⁵⁸⁴⁷)

abnormal neuroanatomy | larval stage (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

abnormal neuroanatomy | third instar larval stage (with Acsl⁸)

Acsl^k10313

abnormal neuroanatomy | third instar larval stage (with Acsl^k10313)

Acsl⁸

abnormal neurophysiology (with Acsl⁰⁵⁸⁴⁷)

Acsl^KO

abnormal neurophysiology (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Acsl^GD1638

embryonic/larval segmentation phenotype (with Df(2R)H3E1)

embryonic/larval segmentation phenotype | maternal effect | recessive

embryonic/larval segmentation phenotype | recessive

Acsl¹

some die during embryonic stage

some die during first instar larval stage | recessive

Acsl^KO

some die during larval stage | recessive

Acsl⁰⁵⁸⁴⁷

some die during pharate adult stage | recessive

Acsl⁰⁵⁸⁴⁷

visible, with Scer\GAL4^pnr-MD237

Acsl^GD1638

Phenotype manifest in

Allele

border follicle cell, with Scer\GAL4^slbo.2.6

Acsl^HMS02307

bouton (with Acsl⁰⁵⁸⁴⁷)

Acsl^KO

bouton (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

embryonic/larval brain | third instar larval stage (with Acsl⁸)

Acsl^k10313

embryonic/larval brain | third instar larval stage (with Acsl^k10313)

Acsl⁸

embryonic/larval neuromuscular junction (with Acsl⁰⁵⁸⁴⁷)

Acsl^KO

embryonic/larval neuromuscular junction (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

embryonic abdominal segment 1

Acsl¹

embryonic abdominal segment 1 | maternal effect

embryonic abdominal segment 2

Acsl¹

embryonic abdominal segment 2 (with Df(2R)H3E1)

Acsl¹

embryonic abdominal segment 2 | maternal effect

embryonic abdominal segment 3

Acsl¹

embryonic abdominal segment 3 (with Df(2R)H3E1)

Acsl¹

embryonic abdominal segment 3 | maternal effect

embryonic abdominal segment 4

Acsl¹

embryonic abdominal segment 4 (with Df(2R)H3E1)

Acsl¹

embryonic abdominal segment 4 | maternal effect

embryonic abdominal segment 5

Acsl¹

embryonic abdominal segment 5 | maternal effect

embryonic abdominal segment 6

Acsl¹

embryonic abdominal segment 6 (with Df(2R)H3E1)

Acsl¹

embryonic abdominal segment 6 | maternal effect

embryonic abdominal segment 7

Acsl¹

embryonic abdominal segment 7 (with Df(2R)H3E1)

Acsl¹

embryonic abdominal segment 7 | maternal effect

embryonic abdominal segment (with Df(2R)H3E1)

Acsl⁸

ganglion mother cell | larval stage

Acsl^GD1638

lamina anlage glial cell (with Acsl⁸)

Acsl^k10313

lamina anlage glial cell (with Acsl^k10313)

Acsl⁸

lamina plexus (with Acsl⁸)

Acsl^k10313

lamina plexus (with Acsl^k10313)

Acsl⁸

larval abdominal 2 neuron (with Acsl⁸)

Acsl⁰⁵⁸⁴⁷

larval abdominal 2 neuron (with Acsl⁰⁵⁸⁴⁷)

larval abdominal 2 neuron (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

larval abdominal 3 neuron (with Acsl⁸)

Acsl⁰⁵⁸⁴⁷

larval abdominal 3 neuron (with Acsl⁰⁵⁸⁴⁷)

larval abdominal 3 neuron (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

larval abdominal 4 neuron (with Acsl⁸)

Acsl⁰⁵⁸⁴⁷

larval abdominal 4 neuron (with Acsl⁰⁵⁸⁴⁷)

larval abdominal 4 neuron (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

larval abdominal 5 neuron (with Acsl⁸)

Acsl⁰⁵⁸⁴⁷

larval abdominal 5 neuron (with Acsl⁰⁵⁸⁴⁷)

larval abdominal 5 neuron (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

larval abdominal 6 neuron (with Acsl⁸)

Acsl⁰⁵⁸⁴⁷

larval abdominal 6 neuron (with Acsl⁰⁵⁸⁴⁷)

larval abdominal 6 neuron (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

larval abdominal 7 neuron (with Acsl⁸)

Acsl⁰⁵⁸⁴⁷

larval abdominal 7 neuron (with Acsl⁰⁵⁸⁴⁷)

larval abdominal 7 neuron (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

larval abdominal segment 6 (with Acsl⁰⁵⁸⁴⁷)

Acsl^KO

larval abdominal segment 6 (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

larval abdominal segment 7 (with Acsl⁰⁵⁸⁴⁷)

Acsl^KO

larval abdominal segment 7 (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

larval neuroblast | larval stage

Acsl^GD1638

larval segmental nerve (with Acsl⁰⁵⁸⁴⁷)

Acsl^KO

larval segmental nerve (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

medulla anlage (with Acsl⁸)

Acsl^k10313

medulla anlage (with Acsl^k10313)

Acsl⁸

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Acsl^GD1638

neuromuscular junction (with Acsl⁸)

Acsl⁰⁵⁸⁴⁷

neuromuscular junction (with Acsl⁰⁵⁸⁴⁷)

neuromuscular junction (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

NMJ bouton (with Acsl⁸)

Acsl⁰⁵⁸⁴⁷

NMJ bouton (with Acsl⁰⁵⁸⁴⁷)

NMJ bouton (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

rhabdomere | conditional, with Scer\GAL4^GMR.PF

rhabdomere | somatic clone | conditional

Acsl^KO

synapse (with Acsl⁰⁵⁸⁴⁷)

Acsl^KO

synapse (with Acsl^KO)

Acsl⁰⁵⁸⁴⁷

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (9)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ACSL3

14 of 15

Yes

Hsap\ACSL4

13 of 15

Yes

3 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Acsl3

13 of 15

Yes

Mmus\Acsl4

12 of 15

Yes

3 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (10)

Rnor\Acsl3

12 of 13

Yes

Rnor\Acsl4

11 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (9)

Xtro\acsl4

5 of 12

Yes

Xtro\acsl3

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (13)

Drer\acsl3a

12 of 15

Yes

Drer\acsl4b

11 of 15

Yes

Drer\acsl3b

10 of 15

Yes

Drer\acsl4a

8 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\acs-4

13 of 15

Yes

Cele\acs-17

12 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (9)

Atha\LACS8

9 of 9

Yes

Atha\LACS9

8 of 9

Yes

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\FAA4

13 of 15

Yes

Scer\FAA1

12 of 15

Yes

Scer\FAA3

12 of 15

Yes

Scer\FAA2

2 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\lcf1

10 of 12

Yes

Spom\lcf2

9 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (19)

Gene Symbol

Score

Source

Alignment

4 of 10

bgm

2 of 10

hll

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

intellectual disability, X-linked 63

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 5 )

Allele

Disease

Evidence

References

Acsl⁸

model of non-syndromic X-linked intellectual disability

CEA with Acsl⁰⁵⁸⁴⁷

(Jia et al., 2019, Zhang et al., 2009)

CEA

(Jia et al., 2019)

model of non-syndromic X-linked intellectual disability 63

CEA with Acsl⁰⁵⁸⁴⁷

(Huang et al., 2016)

Acsl^KO

model of non-syndromic X-linked intellectual disability

CEA with Acsl⁰⁵⁸⁴⁷

(Jia et al., 2019, Liu et al., 2011)

CEA

(Jia et al., 2019)

model of non-syndromic X-linked intellectual disability 63

CEA with Acsl⁰⁵⁸⁴⁷

(Huang et al., 2016)

Acsl⁰⁵⁸⁴⁷

model of non-syndromic X-linked intellectual disability

CEA with Acsl^KO

(Jia et al., 2019, Liu et al., 2011)

CEA with Acsl⁸

(Jia et al., 2019, Zhang et al., 2009)

CEA with Acsl¹

(Jia et al., 2019)

model of non-syndromic X-linked intellectual disability 63

CEA with Acsl⁸

(Huang et al., 2016)

CEA with Acsl^KO

(Huang et al., 2016)

Acsl^JF02811

model of non-syndromic X-linked intellectual disability

CEA

(Jia et al., 2019)

Acsl¹

model of non-syndromic X-linked intellectual disability

CEA with Acsl⁰⁵⁸⁴⁷

(Jia et al., 2019)

CEA

(Jia et al., 2019)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

ACSL4; acyl-CoA synthetase long chain family member 4

model of non-syndromic X-linked intellectual disability 63

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Acsl^KO

model of non-syndromic X-linked intellectual disability

is ameliorated by Acsl^{UAS.715.C.Tag:MYC}

(Jia et al., 2019)

is ameliorated by Hsap\ACSL4^{P375L.UAS.L.Tag:MYC}

(Jia et al., 2019)

Acsl^{UAS.715.C.Tag:MYC}

ameliorates non-syndromic X-linked intellectual disability

modeled by Acsl⁰⁵⁸⁴⁷, Acsl⁸

(Jia et al., 2019)

modeled by Acsl^KO

(Jia et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ACSL3; acyl-CoA synthetase long chain family member 3

14 of 15

602371

ACSL4; acyl-CoA synthetase long chain family member 4

13 of 15

300157

INTELLECTUAL DEVELOPMENTAL DISORDER, X-LINKED 63; XLID63

non-syndromic X-linked intellectual disability 63

ACSL6; acyl-CoA synthetase long chain family member 6

3 of 15

604443

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

Hsap\ACSL3

(Zhang et al., 2009)

View in Interactions Browser

Hsap\ACSL4

(Liu et al., 2014, Liu et al., 2011, Zhang et al., 2009)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Acsl - Cnx99A

(Guruharsha et al., 2011)

Acsl - Mpcp2

anti tag coimmunoprecipitation, western blot

(Guruharsha et al., 2011)

Acsl - Rtnl1

(O'Sullivan et al., 2012)

Acsl - fax

(Guruharsha et al., 2011)

Acsl - msk

View in Interactions Browser

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Liu et al., 2014)

enhanceable

(Zhang et al., 2011)

enhanceable

(Zhang et al., 2011)

enhanceable

(Liu et al., 2014)

suppressible

(Liu et al., 2014)

suppressible

(Liu et al., 2014)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

l(1)sc

enhanceable

(Rottgen et al., 1998)

enhanceable

(Rottgen et al., 1998)

peb

suppressible