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General Information
Symbol
Dmel\Acsl
Species
D. melanogaster
Name
Acyl-CoA synthetase long-chain
Annotation Symbol
CG8732
Feature Type
FlyBase ID
FBgn0263120
Gene Model Status
Stock Availability
Enzyme Name (EC)
Long-chain-fatty-acid--CoA ligase (6.2.1.3)
Gene Summary
Contribute a Gene Snapshot for this gene.
Also Known As

l(2)44DEa, l(2)k05304, dAcsl, l(2)05847, lethal (2) 44DEa

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
2-59
RefSeq locus
NT_033778 REGION:8665813..8683022
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (20 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with HGNC:3570,HGNC:3571
inferred from biological aspect of ancestor with PANTHER:PTN001944821
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001186327
(assigned by GO_Central )
Biological Process (11 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
involved_in axon guidance
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
involved_in segmentation
inferred from mutant phenotype
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000645126
(assigned by GO_Central )
inferred from sequence or structural similarity with HGNC:3570,HGNC:3571
inferred from biological aspect of ancestor with PANTHER:PTN000645126
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001186315
(assigned by GO_Central )
Cellular Component (7 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in axon
inferred from direct assay
located_in cytoplasm
inferred from direct assay
inferred from direct assay
located_in peroxisome
inferred from direct assay
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000644924
(assigned by GO_Central )
is_active_in lipid droplet
inferred from biological aspect of ancestor with PANTHER:PTN000645126
(assigned by GO_Central )
is_active_in membrane
inferred from biological aspect of ancestor with PANTHER:PTN001944821
(assigned by GO_Central )
is_active_in plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000645126
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
-
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA (6.2.1.3)
Summaries
Gene Group (FlyBase)
ACYL-COA SYNTHETASES -
Acyl-coenzyme A (CoA) synthetases catalyze the "activation" of fatty acids by their thioesterification to CoA. This is the initial reaction in fatty acid metabolism, allowing their participation in many fundamental anabolic and catabolic pathways. (Adapted from FBrf0237395).
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
10
Number of Unique Polypeptides
4

Please see the JBrowse view of Dmel\Acsl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A0A0B4KFE4)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0088699
3704
715
FBtr0088700
3677
715
FBtr0088701
3117
707
FBtr0100426
3452
717
FBtr0100427
3852
715
FBtr0100428
3409
707
FBtr0100429
3534
707
FBtr0100430
3887
715
FBtr0100431
3353
707
FBtr0333001
3061
728
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0087779
80.4
715
8.35
FBpp0087780
80.4
715
8.35
FBpp0087781
79.4
707
8.59
FBpp0099845
80.6
717
8.61
FBpp0099846
80.4
715
8.35
FBpp0099847
79.4
707
8.59
FBpp0099848
79.4
707
8.59
FBpp0099849
80.4
715
8.35
FBpp0099850
79.4
707
8.59
FBpp0305216
81.8
728
8.43
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

715 aa isoforms: Acsl-PA, Acsl-PB, Acsl-PE, Acsl-PH
707 aa isoforms: Acsl-PC, Acsl-PF, Acsl-PG, Acsl-PI
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Acsl using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Acsl transcripts are deposited maternally in early embryos.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Acsl protein is ubiquitously distributed in early embryos.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in axon
inferred from direct assay
located_in cytoplasm
inferred from direct assay
inferred from direct assay
located_in peroxisome
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Acsl in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 37 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Acsl
Transgenic constructs containing regulatory region of Acsl
Aberrations (Deficiencies and Duplications) ( 12 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
 
2  
13 of 15
No
Yes
 
4  
3 of 15
No
No
1  
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (10)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
12 of 15
No
Yes
3 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (10)
12 of 13
Yes
Yes
11 of 13
No
Yes
2 of 13
No
No
2 of 13
No
No
2 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (9)
5 of 12
Yes
Yes
3 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (13)
12 of 15
Yes
Yes
11 of 15
No
Yes
10 of 15
No
Yes
8 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (8)
13 of 15
Yes
Yes
12 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (9)
9 of 9
Yes
Yes
8 of 9
No
Yes
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (4)
13 of 15
Yes
Yes
12 of 15
No
Yes
12 of 15
No
Yes
2 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
10 of 12
Yes
Yes
9 of 12
No
Yes
Other Organism Orthologs (via OrthoDB)
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (19)
4 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 5 )
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 2 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Dmel gene
Ortholog showing functional complementation
Supporting References
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
DroID - A comprehensive database of gene and protein interactions.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map
2-59
Cytogenetic map
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
44E1-44D6
Limits computationally determined from genome sequence between P{lacW}Vps25k08904&P{lacW}ptck02507 and P{PZ}l(2)44DEa05847; Left limit from in situ hybridisation (FBrf0157097) Right limit from in situ hybridisation (FBrf0157097)
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
44D3-44D6
44F1-44F2
(determined by in situ hybridisation) 44E1--2 (determined by in situ hybridisation)
44E-44E
(determined by in situ hybridisation)
24D5-24D6
44D6--8 44D8--E1
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (29)
Genomic Clones (17)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (174)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 

polyclonal

Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: Acsl l(2)44DEa

Source for database merge of

Source for merge of: l(2)05847 l(2)k05304

Source for merge of: l(2)44DEa CG8732

Source for merge of: l(2)44DEa anon-WO0172774.173

Source for merge of: Acsl l(2)SH0465

Additional comments

Source for merge of l(2)44DEa anon-WO0172774.173 was sequence comparison ( date:051113 ).

Other Comments

FlyBase Curator comment: 'Acsl' identified as 'FCS' by BLASTing the 'FCS' primer sequences given in the Supplementary Methods.

11 alleles of l(2)SH0465 recovered in a P-insertion screen.

Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

l(2)44DEa may be involved in keeping sister chromatids together or stabilizing the microtubule contact with centromeres.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 91 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL - MARRVEL (model organism gene)
Linkouts
ApoDroso - Functional genomic database for photoreceptor development, survival and function
DroID - A comprehensive database of gene and protein interactions.
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
MIST (protein-protein) - An integrated Molecular Interaction Database
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (29)
Reported As
Symbol Synonym
anon-WO0172774.173
l(2)SH0465
l(2)SH2 0465
Name Synonyms
Acyl-CoA synthetase long-chain
Acyl-CoAsynthetase long-chain
Fatty acyl-CoA synthetase
Long-chain acyl-CoA synthetase
fatty acyl coenzyme A (CoA) synthetase
fatty acyl-CoA synthetase
lethal (2) SH0465
long-chain acyl-CoA synthetase
Secondary FlyBase IDs
  • FBgn0010609
  • FBgn0022182
  • FBgn0033318
  • FBgn0046428
  • FBgn0065770
Datasets (8)
Study focus (0)
Experimental Role
Project
Project Type
Title
Study result (8)
Result
Result Type
Title
Clustering analysis of hemocytes from non-infested third instar larvae
Clustering analysis of hemocytes from wasp-infested third instar larvae
Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
References (129)