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Gene Dmel\Acsl

General Information
Symbol	Dmel\Acsl	Species	D. melanogaster
Name	Acyl-CoA synthetase long-chain	Annotation symbol	CG8732
Feature type	protein_coding_gene	FlyBase ID	FBgn0263120
Gene Model Status	Current	Stock availability	30 publicly available
Also Known As	l(2)44DEa, dAcsl, l(2)k05304, l(2)05847
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	44D3-44D6	Sequence location	2R:4,553,318..4,570,527 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Acyl-CoA synthetase long-chain is referred to in FlyBase by the symbol Dmel\Acsl (CG8732, FBgn0263120). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: long-chain fatty acid-CoA ligase activity. There is experimental evidence that it is involved in the biological process: synaptic transmission; axon guidance; positive regulation of sequestering of triglyceride; segmentation; nervous system development; neurogenesis. 48 alleles are reported. The phenotypes of these alleles are annotated with: multicellular structure; organ system subdivision; organ system; anatomical structure; abdominal segment; larval abdominal segment; larval head segment; peripheral nervous system; adult segment; embryonic abdominal segment 1; synapse. It has 10 annotated transcripts and 10 annotated polypeptides. Protein features are: AMP-binding, conserved site; AMP-dependent synthetase/ligase. Gene sequence location is 2R:4553318..4570527.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 44D3-44D6   Left limit from in situ hybridisation (FBrf0157097) Right limit from in situ hybridisation (FBrf0157097)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 44D3-44D6     (Oh et al., 2003) 44F1-44F2   (determined by in situ hybridisation) 44E1--2 (determined by in situ hybridisation)   (Spradling et al., 1999) 44E-44E   (determined by in situ hybridisation)   (Amorim et al., 1995) 24D5-24D6   44D6--8 44D8--E1   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Acsl for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all supported alternative splices within 5' UTR. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Gene model reviewed during 5.46 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Acsl-RA FBtr0088699  NM_170615   3704   715 Acsl-RB FBtr0088700  NM_143777   3677   715 Acsl-RC FBtr0088701  NM_170616   3117   707 Acsl-RD FBtr0100426  NM_001014512   3452   717 Acsl-RE FBtr0100427  NM_001014511   3852   715 Acsl-RF FBtr0100428  NM_001014510   3409   707 Acsl-RG FBtr0100429  NM_001014509   3534   707 Acsl-RH FBtr0100430  NM_001014508   3887   715 Acsl-RI FBtr0100431  NM_001014507   3353   707 Acsl-RJ FBtr0333001  NM_001273881   3061   728
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Acsl-PA   FBpp0087779   80.4   715   8.35     NP_724695   AAG22300 Acsl-PB   FBpp0087780   80.4   715   8.35     NP_652034   AAF59061 Acsl-PC   FBpp0087781   79.4   707   8.59     NP_724696   AAM68830 Acsl-PD   FBpp0099845   80.6   717   8.61     NP_001014512   AAX52717 Acsl-PE   FBpp0099846   80.4   715   8.35     NP_001014511   AAX52718 Acsl-PF   FBpp0099847   79.4   707   8.59     NP_001014510   AAX52720 Acsl-PG   FBpp0099848   79.4   707   8.59     NP_001014509   AAX52721 Acsl-PH   FBpp0099849   80.4   715   8.35     NP_001014508   AAX52719 Acsl-PI   FBpp0099850   79.4   707   8.59     NP_001014507   AAX52722 Acsl-PJ   FBpp0305216   81.8   728   8.43     NP_001260810   AGB93343
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • AMP-dependent synthetase/ligase (IPR000873) AMP-binding, conserved site (IPR020845)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141432   AA141563   AA141564   AA141939   AA438703   AA697755   AA941158   AE013599 AAF59061   AE013599 AAG22300   AE013599 AAM68830   AE013599 AAX52717   AE013599 AAX52718   AE013599 AAX52719   AE013599 AAX52720   AE013599 AAX52721   AE013599 AAX52722   AB082069   AF006647   AE013599 AGB93343   AI107289   AI109552   AI134179   AI238038   AI256893   AI294048   AI295028   AI403459   AI530881   AI531368   AI531641   AI946131   AW944076   AW944234   AX260211   AY052051 AAK93475   AY094958 AAM11311   AZ301000   BG638031   BG639501   BI165293   BI165836   BI167822   BI169027   BI169229   BI172497   BI173138   BI213486   BI213807   BI213861   BI216194   BI230289   BI231883   BI236785   BI240515   BI241224   BI362083   BI365313   BI367651   BI370613   BI370710   BI371068   BI486490   BI564104   BI570320   BI570383   BI576744   BI589443   BI590523   BI609285   BI611549   BI614273   BI623449   BI624935   BI627707   BI630230   BI634293   BI637348   BP556161   BT003752   BT132993   CA806979   CK658641   CK658926   CO177311   CO178336   CO187668   CO188026   CO261989   CO270193   CO270585   CO273418   CO278309   CO279207   CO279893   CO281038   CO282579   CO285719   CO291114   CO298541   CO298836   CO305836   CO312248   CO312762   CO313369   CO313767   CO313962   CO314568   CO318357   CO324610   CO325169   CZ473939   CZ478430   CZ480451   EC069661   EC072437   EC072700   EC085485   EC088959   EC090411   EC195156   EC197095   EC198378   EC199134   EC203574   EC205686   EC206142   EC206189   EC208705   EC209987   EC214073   EC214804   EC219435   EC225621   EC225747   EC231531   EC232494   EC234038   EC235353   EC238039   EC241657   EC241738   EC245002   EC248106   EC248389   EC248724   EC251871   EV586550   G00494   GH748700   GH755847   GH772970   GH777625   GH782878   GH785285   GH791165   GH810644   GH813956   GH823207   GH825550   GH845040   GH860715   GH861056   GH866029   GH868690   GH873810   GH894385   GH906120   GH907627   GH908406   GH914527   GH922982   GH937437   GH942924   GH944924   GH947417   GH949206   GH949483   GH949604   GH949697   GH949702   GH950779   GH952008   GH954709   GH956219   GH957109   GH958197   GH959776   GH964456   GH966992   GH967041   GH967307   GH968366   GH969641   GH970448   GH970505   GH971008   GH971103   GH971828   GH975793   GH976941   GH978797   GH981546   GH982746   GH983199
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8T3L1 A1Z7H3 A1Z7H2 Q960I0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Zhang et al., 2009, Zhang et al., 2011) endoplasmic reticulum inferred from direct assay (Zhang et al., 2009) lipid particle inferred from direct assay (Cermelli et al., 2006)
Expression Deduced from Reporters
Reporter: P{PZ}Acsl05847 Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage   embryonic/larval fat body   (Meister and Braun, 1995.10) adult stage   third segment of antenna   (Vosshall, 1998.8.10) adult fat body   (Vosshall, 1998.8.10)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    19 central nervous system, pupae P8    22 head, virgin 1-day female    66 head, virgin 4-day female    71 head, virgin 20-day female    89 head, mated 1-day female    50 head, mated 4-day female    91 head, mated 20-day female    75 head, mated 1-day male    77 head, mated 4-day male    81 head, mated 20-day male    110 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    65 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    76 digestive system, 4-day adult    71 digestive system, 20-day adult    68 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    68 carcass, 4-day adult    83 carcass, 20-day adult    79 ovary, virgin 4-day female    60 ovary, mated 4-day female    49 testis, mated 4-day male    19 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    19 central nervous system, pupae P8    22 head, virgin 1-day female  (66) head, virgin 4-day female  (71) head, virgin 20-day female  (89) head, mated 1-day female  (50) head, mated 4-day female  (91) head, mated 20-day female  (75) head, mated 1-day male  (77) head, mated 4-day male  (81) head, mated 20-day male  (110) salivary gland, larvae L3 wandering    3 salivary gland, white prepupae  (65) digestive system, larvae L3 wandering    13 digestive system, 1-day adult  (76) digestive system, 4-day adult  (71) digestive system, 20-day adult  (68) fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult  (68) carcass, 4-day adult  (83) carcass, 20-day adult  (79) ovary, virgin 4-day female  (60) ovary, mated 4-day female  (49) testis, mated 4-day male    19 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    19 central nervous system, pupae P8    22 head, virgin 1-day female    66 head, virgin 4-day female    71 head, virgin 20-day female    89 head, mated 1-day female    50 head, mated 4-day female    91 head, mated 20-day female    75 head, mated 1-day male    77 head, mated 4-day male    81 head, mated 20-day male  (110) salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    65 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    76 digestive system, 4-day adult    71 digestive system, 20-day adult    68 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    68 carcass, 4-day adult    83 carcass, 20-day adult    79 ovary, virgin 4-day female    60 ovary, mated 4-day female    49 testis, mated 4-day male    19 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    19 central nervous system, pupae P8    22 head, virgin 1-day female    66 head, virgin 4-day female    71 head, virgin 20-day female    89 head, mated 1-day female    50 head, mated 4-day female    91 head, mated 20-day female    75 head, mated 1-day male    77 head, mated 4-day male    81 head, mated 20-day male    110 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    65 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    76 digestive system, 4-day adult    71 digestive system, 20-day adult    68 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    68 carcass, 4-day adult    83 carcass, 20-day adult    79 ovary, virgin 4-day female    60 ovary, mated 4-day female    49 testis, mated 4-day male    19 accessory gland, mated 4-day male    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    19 central nervous system, pupae P8    22 head, virgin 1-day female    66 head, virgin 4-day female    71 head, virgin 20-day female    89 head, mated 1-day female    50 head, mated 4-day female    91 head, mated 20-day female    75 head, mated 1-day male    77 head, mated 4-day male    81 head, mated 20-day male    110 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    65 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    76 digestive system, 4-day adult    71 digestive system, 20-day adult    68 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    68 carcass, 4-day adult    83 carcass, 20-day adult    79 ovary, virgin 4-day female    60 ovary, mated 4-day female    49 testis, mated 4-day male    19 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    19 central nervous system, pupae P8    22 head, virgin 1-day female  (66) head, virgin 4-day female  (71) head, virgin 20-day female  (89) head, mated 1-day female  (50) head, mated 4-day female  (91) head, mated 20-day female  (75) head, mated 1-day male  (77) head, mated 4-day male  (81) head, mated 20-day male  (110) salivary gland, larvae L3 wandering    3 salivary gland, white prepupae  (65) digestive system, larvae L3 wandering    13 digestive system, 1-day adult  (76) digestive system, 4-day adult  (71) digestive system, 20-day adult  (68) fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult  (68) carcass, 4-day adult  (83) carcass, 20-day adult  (79) ovary, virgin 4-day female  (60) ovary, mated 4-day female  (49) testis, mated 4-day male    19 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    19 central nervous system, pupae P8    22 head, virgin 1-day female    66 head, virgin 4-day female    71 head, virgin 20-day female    89 head, mated 1-day female    50 head, mated 4-day female    91 head, mated 20-day female    75 head, mated 1-day male    77 head, mated 4-day male    81 head, mated 20-day male  110 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    65 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    76 digestive system, 4-day adult    71 digestive system, 20-day adult    68 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    68 carcass, 4-day adult    83 carcass, 20-day adult    79 ovary, virgin 4-day female    60 ovary, mated 4-day female    49 testis, mated 4-day male    19 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    16 central nervous system, larvae L3    19 central nervous system, pupae P8    22 head, virgin 1-day female    66 head, virgin 4-day female    71 head, virgin 20-day female    89 head, mated 1-day female    50 head, mated 4-day female    91 head, mated 20-day female    75 head, mated 1-day male    77 head, mated 4-day male    81 head, mated 20-day male    110 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    65 digestive system, larvae L3 wandering    13 digestive system, 1-day adult    76 digestive system, 4-day adult    71 digestive system, 20-day adult    68 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    14 carcass, larvae L3 wandering    15 carcass, 1-day adult    68 carcass, 4-day adult    83 carcass, 20-day adult    79 ovary, virgin 4-day female    60 ovary, mated 4-day female    49 testis, mated 4-day male    19 accessory gland, mated 4-day male    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    49 embryo 02-04hr    6 embryo 04-06hr    7 embryo 06-08hr    18 embryo 08-10hr    29 embryo 10-12hr    42 embryo 12-14hr    52 embryo 14-16hr    57 embryo 16-18hr    73 embryo 18-20hr    69 embryo 20-22hr    68 embryo 22-24hr    83 larva L1    73 larva L2    103 larva L3 12hr old    73 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    21 white prepupae new    37 white prepupae 12hr    23 white prepupae 24hr    24 pupae 2d postWPP    45 pupae 3d postWPP    51 pupae 4d postWPP    83 adult male 01day    112 adult male 05day    86 adult male 30day    76 adult female 01day    87 adult female 05day    95 adult female 30day    88 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (49) embryo 02-04hr    6 embryo 04-06hr    7 embryo 06-08hr    18 embryo 08-10hr  (29) embryo 10-12hr  (42) embryo 12-14hr  (52) embryo 14-16hr  (57) embryo 16-18hr  (73) embryo 18-20hr  (69) embryo 20-22hr  (68) embryo 22-24hr  (83) larva L1  (73) larva L2  (103) larva L3 12hr old  (73) larva L3 puffstage 1-2  (29) larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    21 white prepupae new  (37) white prepupae 12hr    23 white prepupae 24hr    24 pupae 2d postWPP  (45) pupae 3d postWPP  (51) pupae 4d postWPP  (83) adult male 01day  (112) adult male 05day  (86) adult male 30day  (76) adult female 01day  (87) adult female 05day  (95) adult female 30day  (88) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    49 embryo 02-04hr    6 embryo 04-06hr    7 embryo 06-08hr    18 embryo 08-10hr    29 embryo 10-12hr    42 embryo 12-14hr    52 embryo 14-16hr    57 embryo 16-18hr    73 embryo 18-20hr    69 embryo 20-22hr    68 embryo 22-24hr    83 larva L1    73 larva L2  103 larva L3 12hr old    73 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    21 white prepupae new    37 white prepupae 12hr    23 white prepupae 24hr    24 pupae 2d postWPP    45 pupae 3d postWPP    51 pupae 4d postWPP    83 adult male 01day  (112) adult male 05day    86 adult male 30day    76 adult female 01day    87 adult female 05day    95 adult female 30day    88 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    49 embryo 02-04hr    6 embryo 04-06hr    7 embryo 06-08hr    18 embryo 08-10hr    29 embryo 10-12hr    42 embryo 12-14hr    52 embryo 14-16hr    57 embryo 16-18hr    73 embryo 18-20hr    69 embryo 20-22hr    68 embryo 22-24hr    83 larva L1    73 larva L2    103 larva L3 12hr old    73 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    21 white prepupae new    37 white prepupae 12hr    23 white prepupae 24hr    24 pupae 2d postWPP    45 pupae 3d postWPP    51 pupae 4d postWPP    83 adult male 01day    112 adult male 05day    86 adult male 30day    76 adult female 01day    87 adult female 05day    95 adult female 30day    88 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    49 embryo 02-04hr    6 embryo 04-06hr    7 embryo 06-08hr    18 embryo 08-10hr    29 embryo 10-12hr    42 embryo 12-14hr    52 embryo 14-16hr    57 embryo 16-18hr    73 embryo 18-20hr    69 embryo 20-22hr    68 embryo 22-24hr    83 larva L1    73 larva L2    103 larva L3 12hr old    73 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    21 white prepupae new    37 white prepupae 12hr    23 white prepupae 24hr    24 pupae 2d postWPP    45 pupae 3d postWPP    51 pupae 4d postWPP    83 adult male 01day    112 adult male 05day    86 adult male 30day    76 adult female 01day    87 adult female 05day    95 adult female 30day    88 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (49) embryo 02-04hr    6 embryo 04-06hr    7 embryo 06-08hr    18 embryo 08-10hr  29 embryo 10-12hr  (42) embryo 12-14hr  (52) embryo 14-16hr  (57) embryo 16-18hr  (73) embryo 18-20hr  (69) embryo 20-22hr  (68) embryo 22-24hr  (83) larva L1  (73) larva L2  (103) larva L3 12hr old  (73) larva L3 puffstage 1-2  29 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    21 white prepupae new  (37) white prepupae 12hr    23 white prepupae 24hr    24 pupae 2d postWPP  (45) pupae 3d postWPP  (51) pupae 4d postWPP  (83) adult male 01day  (112) adult male 05day  (86) adult male 30day  (76) adult female 01day  (87) adult female 05day  (95) adult female 30day  (88) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    49 embryo 02-04hr    6 embryo 04-06hr    7 embryo 06-08hr    18 embryo 08-10hr    29 embryo 10-12hr    42 embryo 12-14hr    52 embryo 14-16hr    57 embryo 16-18hr    73 embryo 18-20hr    69 embryo 20-22hr    68 embryo 22-24hr    83 larva L1    73 larva L2  103 larva L3 12hr old    73 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    21 white prepupae new    37 white prepupae 12hr    23 white prepupae 24hr    24 pupae 2d postWPP    45 pupae 3d postWPP    51 pupae 4d postWPP    83 adult male 01day  112 adult male 05day    86 adult male 30day    76 adult female 01day    87 adult female 05day    95 adult female 30day    88 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    49 embryo 02-04hr    6 embryo 04-06hr    7 embryo 06-08hr    18 embryo 08-10hr    29 embryo 10-12hr    42 embryo 12-14hr    52 embryo 14-16hr    57 embryo 16-18hr    73 embryo 18-20hr    69 embryo 20-22hr    68 embryo 22-24hr    83 larva L1    73 larva L2    103 larva L3 12hr old    73 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    19 larva L3 puffstage 7-9    21 white prepupae new    37 white prepupae 12hr    23 white prepupae 24hr    24 pupae 2d postWPP    45 pupae 3d postWPP    51 pupae 4d postWPP    83 adult male 01day    112 adult male 05day    86 adult male 30day    76 adult female 01day    87 adult female 05day    95 adult female 30day    88 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    47 embryonic 1182-4H    70 embryonic GM2    79 embryonic Kc167    44 embryonic S1    23 embryonic S3    72 leg disc CME L1    45 wing disc CME-W2    35 wing disc ML-DmD8    54 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    7 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    92 tumorous blood cells mbn2    74 ovary fGS/OSS    20 ovary OSC    15 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (44) Schneider line 2 Sg4  (47) embryonic 1182-4H  (70) embryonic GM2  (79) embryonic Kc167  (44) embryonic S1    23 embryonic S3  (72) leg disc CME L1  (45) wing disc CME-W2  (35) wing disc ML-DmD8  (54) wing disc ML-DmD9  (27) wing disc ML-DmD16-c3  (30) wing disc ML-DmD21    7 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3  (27) eye-antennal disc ML-DmD11  26 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1  (61) CNS ML-DmBG1-c1  (32) CNS ML-DmBG2-c2  (92) tumorous blood cells mbn2  (74) ovary fGS/OSS    20 ovary OSC    15 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    47 embryonic 1182-4H    70 embryonic GM2    79 embryonic Kc167    44 embryonic S1    23 embryonic S3    72 leg disc CME L1    45 wing disc CME-W2    35 wing disc ML-DmD8    54 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    7 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    92 tumorous blood cells mbn2    74 ovary fGS/OSS    20 ovary OSC    15 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    47 embryonic 1182-4H    70 embryonic GM2    79 embryonic Kc167    44 embryonic S1    23 embryonic S3    72 leg disc CME L1    45 wing disc CME-W2    35 wing disc ML-DmD8    54 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    7 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    92 tumorous blood cells mbn2    74 ovary fGS/OSS    20 ovary OSC    15 ovary OSS    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    47 embryonic 1182-4H    70 embryonic GM2    79 embryonic Kc167    44 embryonic S1    23 embryonic S3    72 leg disc CME L1    45 wing disc CME-W2    35 wing disc ML-DmD8    54 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    7 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    92 tumorous blood cells mbn2    74 ovary fGS/OSS    20 ovary OSC    15 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (44) Schneider line 2 Sg4  (47) embryonic 1182-4H  (70) embryonic GM2  (79) embryonic Kc167  (44) embryonic S1    23 embryonic S3  (72) leg disc CME L1  (45) wing disc CME-W2  (35) wing disc ML-DmD8  (54) wing disc ML-DmD9  27 wing disc ML-DmD16-c3  30 wing disc ML-DmD21    7 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3  27 eye-antennal disc ML-DmD11  26 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1  (61) CNS ML-DmBG1-c1  32 CNS ML-DmBG2-c2  (92) tumorous blood cells mbn2  (74) ovary fGS/OSS    20 ovary OSC    15 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    47 embryonic 1182-4H    70 embryonic GM2    79 embryonic Kc167    44 embryonic S1    23 embryonic S3    72 leg disc CME L1    45 wing disc CME-W2    35 wing disc ML-DmD8    54 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    7 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    92 tumorous blood cells mbn2    74 ovary fGS/OSS    20 ovary OSC    15 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    44 Schneider line 2 Sg4    47 embryonic 1182-4H    70 embryonic GM2    79 embryonic Kc167    44 embryonic S1    23 embryonic S3    72 leg disc CME L1    45 wing disc CME-W2    35 wing disc ML-DmD8    54 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    7 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    13 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    92 tumorous blood cells mbn2    74 ovary fGS/OSS    20 ovary OSC    15 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    18 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    47 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    35 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    29 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    51 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    60 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    32 Rotenone 8 μg 12 hrs, larvae L3    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    18 heat shock, 4-day adult  (43) Cadmium 50 mM 6 hrs, larvae L3  26 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult  (32) Cadmium 100 mM 48 hrs, 4-day adult  (44) Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult  (47) Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult  (39) Ethanol 2.5% 3 hrs, larvae L3  (60) Ethanol 5% 3 hrs, larvae L3  (35) Ethanol 10% 3 hrs, larvae L3  (28) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (29) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (51) Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult  (60) Paraquat 10 mM 48 hrs, 4-day adult  (54) Rotenone 2 μg 12 hrs, larvae L3  (32) Rotenone 8 μg 12 hrs, larvae L3    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    18 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    47 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    35 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    29 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    51 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    60 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    32 Rotenone 8 μg 12 hrs, larvae L3    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    18 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    47 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    35 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    29 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    51 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    60 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    32 Rotenone 8 μg 12 hrs, larvae L3    21 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    18 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    47 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    35 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    29 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    51 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    60 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    32 Rotenone 8 μg 12 hrs, larvae L3    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    18 heat shock, 4-day adult  (43) Cadmium 50 mM 6 hrs, larvae L3  26 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult  32 Cadmium 100 mM 48 hrs, 4-day adult  (44) Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult  (47) Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult  (39) Ethanol 2.5% 3 hrs, larvae L3  (60) Ethanol 5% 3 hrs, larvae L3  (35) Ethanol 10% 3 hrs, larvae L3  28 Caffeine 1.5 mg/ml 4 hrs, larvae L3  29 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (51) Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult  (60) Paraquat 10 mM 48 hrs, 4-day adult  (54) Rotenone 2 μg 12 hrs, larvae L3  32 Rotenone 8 μg 12 hrs, larvae L3    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    18 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    47 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    35 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    29 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    51 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    60 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    32 Rotenone 8 μg 12 hrs, larvae L3    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    18 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    32 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    21 Copper 15 mM 48 hrs, 4-day adult    47 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    39 Ethanol 2.5% 3 hrs, larvae L3    60 Ethanol 5% 3 hrs, larvae L3    35 Ethanol 10% 3 hrs, larvae L3    28 Caffeine 1.5 mg/ml 4 hrs, larvae L3    29 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    51 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    60 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    32 Rotenone 8 μg 12 hrs, larvae L3    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_20 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • SD02373
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal AcslGD1638 lethal (with Acsl1) Acsl8 lethal (with Acsl8) Acsl1 Acsl05847 lethal (with Acsl05847) Acsl8 lethal | embryonic stage Acsl1 Acsl2 Acsl3 Acsl4 lethal | first instar larval stage | recessive AcslKO lethal | larval stage | recessive Acsl05847 lethal | pharate adult stage | recessive Acsl05847 lethal | recessive Acsl1 Acsl5 Acsl6 Acsl7 Acsl8 Acsl05847 Acsl07447 Acslk05304 Acslk05512 Acslk10313 Acslk16116 AcslSH0465 viable, with Scer\GAL4pnr-MD237 AcslGD1638 Other Phenotypes Allele embryonic/larval segmentation phenotype (with Df(2R)H3E1) Acsl1 Acsl8 embryonic/larval segmentation phenotype | maternal effect | recessive Acsl1 Acsl8 embryonic/larval segmentation phenotype | recessive Acsl1 mitotic cell cycle defective | recessive Acsl05847 neuroanatomy defective | larval stage AcslGD1638 neuroanatomy defective | larval stage (with Acsl05847) AcslKO neuroanatomy defective | larval stage (with AcslKO) Acsl05847 neuroanatomy defective | third instar larval stage (with Acsl8) Acslk10313 neuroanatomy defective | third instar larval stage (with Acslk10313) Acsl8 neurophysiology defective (with Acsl05847) AcslKO neurophysiology defective (with AcslKO) Acsl05847 visible, with Scer\GAL4pnr-MD237 AcslGD1638 Phenotype manifest in Allele bouton (with Acsl05847) AcslKO bouton (with AcslKO) Acsl05847 embryonic/larval brain | third instar larval stage (with Acsl8) Acslk10313 embryonic/larval brain | third instar larval stage (with Acslk10313) Acsl8 embryonic/larval neuroblast | larval stage AcslGD1638 embryonic/larval neuromuscular junction (with Acsl05847) AcslKO embryonic/larval neuromuscular junction (with AcslKO) Acsl05847 embryonic abdominal segment 1 Acsl1 embryonic abdominal segment 1 | maternal effect Acsl8 Acsl1 embryonic abdominal segment 2 Acsl1 embryonic abdominal segment 2 (with Df(2R)H3E1) Acsl1 embryonic abdominal segment 2 | maternal effect Acsl8 Acsl1 embryonic abdominal segment 3 Acsl1 embryonic abdominal segment 3 (with Df(2R)H3E1) Acsl1 embryonic abdominal segment 3 | maternal effect Acsl8 Acsl1 embryonic abdominal segment 4 Acsl1 embryonic abdominal segment 4 (with Df(2R)H3E1) Acsl1 embryonic abdominal segment 4 | maternal effect Acsl8 Acsl1 embryonic abdominal segment 5 Acsl1 embryonic abdominal segment 5 | maternal effect Acsl8 Acsl1 embryonic abdominal segment 6 Acsl1 embryonic abdominal segment 6 (with Df(2R)H3E1) Acsl1 embryonic abdominal segment 6 | maternal effect Acsl8 Acsl1 embryonic abdominal segment 7 Acsl1 embryonic abdominal segment 7 (with Df(2R)H3E1) Acsl1 embryonic abdominal segment 7 | maternal effect Acsl8 Acsl1 embryonic abdominal segment (with Df(2R)H3E1) Acsl8 ganglion mother cell | larval stage AcslGD1638 lamina anlage glial cell (with Acsl8) Acslk10313 lamina anlage glial cell (with Acslk10313) Acsl8 lamina plexus (with Acsl8) Acslk10313 lamina plexus (with Acslk10313) Acsl8 larval abdominal segment 6 (with Acsl05847) AcslKO larval abdominal segment 6 (with AcslKO) Acsl05847 larval abdominal segment 7 (with Acsl05847) AcslKO larval abdominal segment 7 (with AcslKO) Acsl05847 medulla anlage (with Acsl8) Acslk10313 medulla anlage (with Acslk10313) Acsl8 mesothoracic tergum, with Scer\GAL4pnr-MD237 AcslGD1638 segmental nerve (with Acsl05847) AcslKO segmental nerve (with AcslKO) Acsl05847 synapse (with Acsl05847) AcslKO synapse (with AcslKO) Acsl05847
Classical Alleles ( 35 )
For All Classical Alleles Show
Allele of Acsl Class Mutagen Stocks Known lesion Acsl05847 P-element activity 2 Yes Acsl8 ethyl methanesulfonate 2 Yes Acslk10313 P-element activity 2 Yes Acsl1 ethyl methanesulfonate 1 Yes Acslc02001 1 -- AcslCB-5220-3 1 -- AcslCB-6209-3 1 -- Acsld04457 P-element activity 1 -- Acsld06514 1 -- Acsle02676 piggyBac transposase 1 -- AcslEY07112 1 -- Acslk05304 P-element activity 1 -- Acslk05512 P-element activity 1 -- Acslk06526 P-element activity 1 -- Acslk10306 P-element activity 1 -- Acslk16116 P-element activity 1 -- AcslKO loss of function allele FLPase 1 Yes AcslMI02966 1 -- AcslNP0553 1 -- AcslNP1202 1 -- AcslNP2479 1 -- AcslNP5914 1 -- Acsl07447 P-element activity 0 -- Acsl2 ethyl methanesulfonate 0 -- Acsl3 ethyl methanesulfonate 0 -- Acsl4 ethyl methanesulfonate 0 -- Acsl5 ethyl methanesulfonate 0 -- Acsl6 ethyl methanesulfonate 0 -- Acsl7 ethyl methanesulfonate 0 -- AcslDG05805 P-element activity 0 -- Acsle00553 piggyBac transposase 0 -- AcslE305-3 ethyl methanesulfonate 0 -- AcslE481-3 ethyl methanesulfonate 0 -- AcslEP2365 P-element activity 0 Yes AcslSH0465 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Acsl Class Mutagen Stocks Known lesion AcslGD1638 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AcslGLC01457 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AcslHMS02307 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AcslJF02811 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AcslKK108893 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AcslScer\UAS.715.C.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes AcsldsRNA.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes AcslNIG.8732R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes AcslScer\UAS.707.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes AcslScer\UAS.715.N.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes AcslScer\UAS.717.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes AcslΔC.Scer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes AcslΔN.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(2L)ed1 (BDGP Project Members, 1994-1999) Df(2L)ed-dp (BDGP Project Members, 1994-1999) Df(2R)cn9 (BDGP Project Members, 1994-1999) Df(2R)G75 (Mohr and Boswell, 2002, BDGP Project Members, 1994-1999) Df(2R)H3C1 (BDGP Project Members, 1994-1999, Spradling et al., 1999) Df(2R)Np3 (Mohr and Boswell, 2002) Df(2R)Np5 (Mohr and Boswell, 2002) Df(2R)w45-30n (Mohr and Boswell, 2002) Df(2R)w73-2 (Mohr and Boswell, 2002)
Disrupted in	Df(2R)44CE (Spradling et al., 1999, Mohr and Boswell, 2002, BDGP Project Members, 1994-1999) Df(2R)Exel7098 (Ryder, 2004.4.26) Df(2R)H3E1 (Mohr and Boswell, 2002, Dockendorff et al., 2000, Zhang et al., 2009, Liu et al., 2011) Df(2R)Np3 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.8732R} NA P{TRiP.GLC01457} NA P{TRiP.HMS02307} NA P{UAS-Acsl.707.Myc} NA P{UAS-Acsl.715.MycC} NA P{UAS-Acsl.715.MycN} NA P{UAS-Acsl.717.Myc} NA P{UAS-Acsl.RNAi} NA P{UAS-Acsl.ΔC} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Acslk05304 No P{lacW}Acslk05512 No P{lacW}Acslk06526 No P{lacW}Acslk10306 No P{lacW}Acslk10313 No P{lacW}Acslk16116 No P{lacW}AcslSH0465 No P{PZ}Acsl05847 No P{PZ}Acsl07447 No insertion of mobile activating element P{EP}AcslEP2365 NA P{EPgy2}AcslEY07112 NA P{GSV1}GSd423GSd423 NA P{XP}Acsld04457 NA P{XP}Acsld06514 NA miscellaneous insertions Mi{MIC}AcslMI02966 NA P{RS3}AcslCB-5220-3 NA P{RS3}AcslCB-6209-3 NA P{wHy}AcslDG05805 NA PBac{PB}Acslc02001 NA PBac{RB}Acsle00553 NA PBac{RB}Acsle02676 NA insertion of enhancer trap binary system P{GawB}AcslNP0553 No P{GawB}AcslNP1202 No P{GawB}AcslNP2479 No P{GawB}AcslNP5914 No
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Zhang et al., 2009) nervous system development inferred from mutant phenotype (Zhang et al., 2009) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) positive regulation of sequestering of triglyceride inferred from mutant phenotype (Zhang et al., 2009) segmentation inferred from mutant phenotype (Zhang et al., 2011) synaptic transmission inferred from mutant phenotype (Liu et al., 2011)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Zhang et al., 2009, Zhang et al., 2011) endoplasmic reticulum inferred from direct assay (Zhang et al., 2009) lipid particle inferred from direct assay (Cermelli et al., 2006)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
long-chain fatty acid-CoA ligase activity inferred from sequence or structural similarity with UniProtKB:O35547 (FlyBase, 1992-)
Biological Process
CV term Evidence References
metabolic process inferred from electronic annotation with InterPro:IPR000873 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Acsl - CG1907 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - CG33970 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - CG3565 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - CG3570 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - CG5196 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - CSN4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - Cnx99A experimental knowledge based (Guruharsha et al., 2011) Acsl - Esp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - Gyk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - Men anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - Mpcp experimental knowledge based (Guruharsha et al., 2011) Acsl - Rpp30 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - Teh1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - Trap1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - fax experimental knowledge based (Guruharsha et al., 2011) Acsl - lic anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Acsl - msk experimental knowledge based (Guruharsha et al., 2011) Acsl - nemy anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Acsl allele Gene References l(2)44DEa05847 peb (Wilk et al., 2004) l(2)44DEaE305-3 N (Rottgen et al., 1998) l(2)44DEak10313 peb (Wilk et al., 2004) unspecified l(1)sc (Rottgen et al., 1998)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0263120
Orthologs
OrthoDB Orthologs (52) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6ZGPXR Dipteran inclusive ortholog search EOG6V9TZR Insect inclusive ortholog search EOG6FJ74X Arthropod inclusive ortholog search EOG6C2FRN Metazoa inclusive ortholog search EOG68DCN9
Orthologs in Drosophila Species (EOG6ZGPXR)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Acsl     Drosophila simulans   Dsim\GD10588 Y   Drosophila sechellia   Dsec\GM21055 Y   Drosophila erecta   Dere\GG23376 Y   Drosophila yakuba   Dyak\GE19217 Y   Drosophila ananassae   Dana\GF12373 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21288 Y   Drosophila persimilis   Dper\GL17113 Y   Drosophila willistoni   Dwil\GK21733 Y   Drosophila virilis   Dvir\GJ21833 Y   Drosophila mojavensis   Dmoj\GI18805 Y   Drosophila grimshawi   Dgri\GH20089 Y
Orthologs in non-Drosophila Dipterans (EOG6V9TZR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010991   Anopheles gambiae Malaria mosquito  Agam\AGAP003623   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ019378   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015739
Orthologs in non-Dipteran Insects (EOG6FJ74X)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB42732   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG011024   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH27080   Atta cephalotes Leafcutter ant  Acep\ACEP17894   Camponotus floridanus Florida carpenter ant  Cflo\CFLO12851   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21511   Linepithema humile Argentine ant  Lhum\LH11911   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18527   Solenopsis invicta Red fire ant  Sinv\SINV17265   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001408   Bombyx mori Silkmoth  Bmor\BGIBMGA003788   Pediculus humanus Human body louse  Phum\PHUM509490   Tribolium castaneum Red flour beetle  Tcas\TC014440
Orthologs in non-Insect Arthropods (EOG6C2FRN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\PEPCK   Daphnia pulex Water flea  Dpux\JGI_V11_213488   Daphnia pulex Water flea  Dpux\Prss30   Ixodes scapularis Deer tick  Isca\ISCW003216   Ixodes scapularis Deer tick  Isca\ISCW000319   Ixodes scapularis Deer tick  Isca\ISCW004612
Orthologs in non-Arthropod Metazoa (EOG68DCN9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\acs-4   Caenorhabditis elegans Nematode  Cele\acs-17   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_013912gn   Danio rerio Zebrafish  Drer\acsl4a   Danio rerio Zebrafish  Drer\acsl3a   Danio rerio Zebrafish  Drer\acsl4l   Danio rerio Zebrafish  Drer\acsl3b   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000033126   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000012429   Gallus gallus Domestic chicken  Ggal\ACSL4   Gallus gallus Domestic chicken  Ggal\ACSL3   Mus musculus House mouse  Mmus\Acsl4   Mus musculus House mouse  Mmus\Acsl3   Rattus norvegicus Norway rat  Rnor\Acsl4   Rattus norvegicus Norway rat  Rnor\Acsl3   Homo sapiens Human  Hsap\ACSL3   Homo sapiens Human  Hsap\ACSL4
Human Orthologs (2)
Gene OMIM HGNC Hsap\ACSL3     Hsap\ACSL4
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10588 Drosophila sechellia Dsec\GM21055 Drosophila erecta Dere\GG23376 Drosophila yakuba Dyak\GE19217 Drosophila ananassae Dana\GF12373 Drosophila pseudoobscura pseudoobscura Dpse\GA21288 Drosophila persimilis Dper\GL17113 Drosophila willistoni Dwil\GK21733 Drosophila virilis Dvir\GJ21833 Drosophila mojavensis Dmoj\GI18805 Drosophila grimshawi Dgri\GH20089
Stocks & Reagents
Stocks Listed in FlyBase ( 30 )
Bloomington	5495 Acsl1/CyO 5496 Acsl8/CyO
Harvard	- P{XP}Acsld04457 - P{XP}Acsld06514 - PBac{PB}Acslc02001 - PBac{RB}Acsle02676
Kyoto	123513 w1118; P{RS3}AcslCB-5220-3 123976 w1118; P{RS3}AcslCB-6209-3 103648 w*; P{GawB}AcslNP0553 / CyO 112937 w*; P{GawB}AcslNP2479 / CyO
VDRC	v101504 P{KK108893}VIE-260B v3222 w1118; P{GD1638}v3222
	More stocks available...
Genomic Clones ( 2 )
	BACR21H10 BACR43I04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 177 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP07340 RH17880 SD02373
Other clones	DPiM_SD02373 MIP33466
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH03867 GH08777 GH11560 GH14022 GH22902 HL03107 LD25142 LP03593 LP08540 LP15742 LP16253 RE05152 RE05810 RE08302 RE08967 RE09983 RE10341 RE15009 RE15881 RE18894 RE19414 RE19485 RE23136 RE24840 RE32904 RE37368 RE38249 RE46553 RE50576 RE53536 RE57313 RE57434 RE57882 RE69927 RH02626 RH02695 RH04155 RH14047 RH33371 RH33880 RH35805 RH38028 RH43615 RH55705 RH59231 RH64691 RH69085 SD02831 SD19334
Other clones	11100 17233 18935 28564 31006 37183 37247 44897 55421 67625 101347 104983 106671 107984 108248 117843 147037 148431 148569 148639 193848 214375.19 CK01.12D.C11 CK01.14A.D2 CK01.36D.D11 CK01.65B.H5 CK01.71A.B4 CK01.74C.C3 CK01.98C.H6 CK01.110A.G4 CK01.114C.G5 CK01.127C.E12 CK00041 CK01738 CK02034 Dmel_neb_013709_0159_2610 EK031216 EK036231 EK053131 EK056438 EK060506 EK076148 EK083358 EK097839 EK099358 EK110863 EK129717 EK133763 EK136328 EK155814 EK160749 EK173221 EK173946 EK194350 EK195736 EK228251 EK229932 EK232442 EK234025 EK234512 EK237033 EK249528 EK271802 ESG015d.E21.1_C10 FGM301G04 LD13036 RP003007055 RP003031497 RP003069585 RP003097165 RP003113182 RP003124048 RP003136372 RP003140635 RP003142969 RP003148993 RP003211961 RP003224973 RP003229614 RP003229912 RP003247123 RP003277669 RP003298701 RP003315192 RP003327292 RP003329950 RP003497355 RP003557708 RP003591992 RP003628984 RP004040563 RP004065760 RP004083219 RP004110034 RP004150033 RP004164322 RP004181468 RP004181514 RP004183666 RP004184346 RP004187012 RP004209263 RP004212974 RP004217678 RP004230231 RP004234701 RP004254184 RP004255780 RP004306740 RP004334422 RP004406658 RP004417781 RP004418981 RP004426017 RP004427145 RP004438638 RP004455620 RP004469083 RP004472926 RP004473053 RP004503622 RP004552805 SD01640
RNAi & Array Information
Linkouts	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 46068
Antibody Information
	polyclonal (Zhang et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Acsl l(2)44DEa (Zhang et al., 2009)
Source for database merge of	Source for merge of: Acsl l(2)SH0465 (Liu et al., 2011) Source for merge of: l(2)44DEa anon-WO0172774.173 (FlyBase, 1996-) Source for merge of: l(2)44DEa CG8732 (Misra, 2000.11.18) Source for merge of: l(2)05847 l(2)k05304 (Dockendorff et al., 2000)
Additional comments	Source for merge of l(2)44DEa anon-WO0172774.173 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	FlyBase Curator comment: 'Acsl' identified as 'FCS' by BLASTing the 'FCS' primer sequences given in the Supplementary Methods. (Bauer et al., 2009) 11 alleles of l(2)SH0465 recovered in a P-insertion screen. (Oh et al., 2003) Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland. (Harvie et al., 1998) l(2)44DEa may be involved in keeping sister chromatids together or stabilizing the microtubule contact with centromeres. (Amorim et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143777 NM_170615 NM_170616 NM_001014507 NM_001014508 NM_001014509 NM_001014510 NM_001014511 NM_001014512 NM_001273881 RefSeq (Proteins) • NP_652034 NP_724695 NP_724696 NP_001014507 NP_001014508 NP_001014509 NP_001014510 NP_001014511 NP_001014512 NP_001260810 DDBJ / EMBL / GenBank • AAF59061 AAF59061.2 AAG22300 AAG22300.2 AAK93475 AAM11311 AAM68830 AAM68830.1 AAX52717 AAX52718 AAX52719 AAX52720 AAX52721 AAX52722 AB082069 AF006647 AGB93343 AI238038 AI294048 AI531368 AI946131 AW944076 AW944234 AX260211 AY052051 AY094958 AZ301000 BI611549 CZ473939 CZ478430 CZ480451 G00494 Entrez Gene - A searchable database of RefSeq genes. • 46068 UniProtKB/TrEMBL • A1Z7H2 A1Z7H3 Q8T3L1 Q960I0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • AMP-dependent synthetase/ligase (IPR000873) AMP-binding, conserved site (IPR020845) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • SD02373
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0263120 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 46068
Synonyms & Secondary IDs ( 23 )
Reported As
Symbol Synonym	7447 (Harvie et al., 1998) Acsl (Zhang and Wang, 2010.11.5, Neumüller et al., 2011) anon-WO0172774.173   CG8732 (Sala et al., 2011, Seegmiller et al., 2002, Cermelli et al., 2006, Quinones-Coello, 2007, Guo et al., 2008, Montooth et al., 2006, Keleman et al., 2009.8.5, Scherzer et al., 2003, Perkins et al., 2009, Oba et al., 2005, Cammarato et al., 2011, Ni et al., 2010.12.1) dAcsl (Liu et al., 2011, Zhang et al., 2009, Zhang et al., 2011) dFAC (Lee et al., 2010) FACS (Guo et al., 2008) FCS (Bauer et al., 2009) l(2)44DEa (Li et al., 2007, FlyBase, 1992-, Cermelli et al., 2006, Stuart et al., 2007, Quinones-Coello, 2007, Minidorff et al., 2007, Liu and Lehmann, 2008, Japanese National Institute of Genetics, 2012.5.21, Siddiqui et al., 2012, Smibert et al., 2012) l(2)05847 (Rottgen et al., 1998, Amorim et al., 1995, ) l(2)7447 (Harvie, 1997.6.4) l(2)k05304 (Dockendorff et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999, ) l(2)SH2 0465 (Oh et al., 2002.3.22) l(2)SH0465
Name Synonym	Acyl-CoA synthetase long-chain   Fatty acyl CoA synthetase (Rawson, 2003, Seegmiller et al., 2002, Seegmiller et al., 2002) Fatty acyl-CoA synthetase (Dobrosotskaya et al., 2002) fatty acyl-CoA synthetase (Bauer et al., 2009) fatty acyl coenzyme A (CoA) synthetase (Lee et al., 2010) lethal (2) 44DEa (Cammarato et al., 2011) lethal (2) SH0465   Long-chain acyl-CoA synthetase (Zhang et al., 2009) long-chain acyl-CoA synthetase (Zhang et al., 2011)
Secondary FlyBase IDs
	FBgn0010609 FBgn0022182 FBgn0033318 FBgn0046428 FBgn0065770
References ( 73 )
Generate a list of	All references relating to this gene ( 73 )
List References by type	Research paper  ( 27 ) Supplementary material  ( 13 ) Review  ( 1 ) Personal communication to FlyBase  ( 21 ) Abstract  ( 1 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Patent  ( 1 )
Recent research papers ( 6 )
	O'Sullivan et al., 2012, Hum. Mol. Genet. 21(15): 3356--3365 Reticulon-like-1, the Drosophila orthologue of the Hereditary Spastic Paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons. [FBrf0218849] Siddiqui et al., 2012, Genome Biol. 13(2): R11 Genome-wide analysis of the maternal-to-zygotic transition in Drosophila primordial germ cells. [FBrf0218847] Liu et al., 2011, J. Neurosci. 31(6): 2052--2063 Drosophila Acyl-CoA Synthetase Long-Chain Family Member 4 Regulates Axonal Transport of Synaptic Vesicles and Is Required for Synaptic Development and Transmission. [FBrf0212968] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Sala et al., 2011, EMBO J. 30(9): 1766--1777 Genome-wide characterization of chromatin binding and nucleosome spacing activity of the nucleosome remodelling ATPase ISWI. [FBrf0213629] Zhang et al., 2011, Dev. Biol. 353(2): 259--265 Drosophila long-chain acyl-CoA synthetase acts like a gap gene in embryonic segmentation. [FBrf0213522] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...