Gene Dmel\Acn

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

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Nucleotide-binding, alpha-beta plait (IPR012677)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

(The modENCODE Consortium, 2010)

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Notes

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Notes

High-Throughput Expression Data

Untitled Document detailed view

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

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modENCODE Temporal Expression Data (Graveley et al., 2011)
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

mE2_34_mRNA_expression_cluster_30

External Data & Images

eye color defective | somatic clone

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

Acn²

lethal, with Scer\GAL4^tub.PU

Acn^{Scer\UAS.T:Hsap\MYC}

lethal (with Acn¹)

Acn²⁷

lethal (with Acn²⁷)

Acn¹

lethal (with Df(2L)TW130)

lethal | pharate adult stage

Acn⁴

viable

Acn^fs1

viable, with Scer\GAL4^pnr-MD237

Acn^GD6985

Sterility

Allele

female sterile

female sterile | recessive

male sterile

male sterile | recessive

Other Phenotypes

Allele

Acn²⁷

melanotic mass phenotype

Acn⁴

wild-type

Phenotype manifest in

Allele

adult cuticle & abdomen

cuticle & abdomen & pharate adult

embryonic/larval fat body, with Scer\GAL4^Lsp2.PH

Acn^{Scer\UAS.T:Hsap\MYC}

embryonic/larval fat body (with Acn¹)

Acn²⁷

embryonic/larval fat body (with Acn²⁷)

Acn¹

eye | somatic clone

Acn²⁷

eye photoreceptor cell | somatic clone

Acn²⁷

melanotic mass

Acn⁴

ommatidium | somatic clone

Acn²⁷

pigment cell | somatic clone

Acn²⁷

Classical Alleles ( 16 )

For All Classical Alleles Show

Allele of Acn	Class	Mutagen	Stocks	Known lesion
Acn¹	loss of function allele	ethyl methanesulfonate	1	Yes
Acn^EY22500		P-element activity	1	--
Acn^fs1		ethyl methanesulfonate	1	--
Acn¹⁰		ethyl methanesulfonate	0	--
Acn¹¹		ethyl methanesulfonate gamma ray	0	--
Acn¹²		ethyl methanesulfonate gamma ray	0	--
Acn²⁷	loss of function allele	ethyl methanesulfonate	0	Yes
Acn²		ethyl methanesulfonate formaldehyde	0	--
Acn³		ethyl methanesulfonate gamma ray	0	--
Acn⁴		ethyl methanesulfonate	0	--
Acn⁵			0	--
Acn⁶		ethyl methanesulfonate	0	--
Acn⁷		ethyl methanesulfonate	0	--
Acn⁸		ethyl methanesulfonate gamma ray	0	--
Acn⁹		ethyl methanesulfonate	0	--
Acn^GS5198			0	Yes

Alleles Carried on Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Acn	Mutagen	Stocks	Known lesion
Acn^GD6985	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Acn^KK111555	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Acn^+t4.2	in vitro construct - genomic fragment	0	Yes
Acn^+t6.2	in vitro construct - genomic fragment	0	Yes
Acn^+t8.7	in vitro construct - genomic fragment	0	Yes
Acn^{4.2.T:Hsap\MYC}	in vitro construct - genomic fragment	0	Yes
Acn^B1	in vitro construct - genomic fragment	0	Yes
Acn^B2	in vitro construct - genomic fragment	0	Yes
Acn^B3	in vitro construct - genomic fragment	0	Yes
Acn^B7	in vitro construct - genomic fragment	0	Yes
Acn^{Scer\UAS.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)E71

(Wright et al., 1981)

Df(2L)Exel6042

(Ryder, 2004.4.26)

Df(2L)hk18

(Wright, 1996, Pentz et al., 1986)

Df(2L)hk-UC1

(Gilbert et al., 1984, Pentz et al., 1986, Stathakis et al., 1995, Wright, 1996)

Df(2L)hk-UC2

(Stathakis et al., 1995, Wright, 1996)

Df(2L)OD15

(Wright, 1996, Pentz et al., 1986, Stathakis et al., 1995)

Df(2L)TW130

(Wright et al., 1981, Wright, 1996, Stathakis et al., 1995)

Df(2L)VA18

Df(2L)VA21

Df(2L)VA23

Not disrupted in

Df(2L)Catsup⁴⁴

(Stathakis et al., 1999)

Df(2L)Catsup⁴⁵

(Stathakis et al., 1999)

Df(2L)Catsup⁴⁶

(Stathakis et al., 1999)

Df(2L)E55

Df(2L)NST

(Wright, 1996, Stathakis et al., 1995, Pentz et al., 1986)

Df(2L)Sd37

Df(2L)Sd57

(Stathakis et al., 1995, Wright, 1996)

Df(2L)Sd77

Df(2L)TE37C-1

(Gilbert et al., 1984, Wright et al., 1981, Wright, 1996)

Df(2L)TE37C-7

Df(2L)TW137

(Wright, 1996, Stathakis et al., 1995, Gilbert et al., 1984, Pentz et al., 1986, Wright et al., 1981)

Df(2L)TW203

(Stathakis et al., 1995, Wright, 1996, Pentz et al., 1986)

Df(2L)VA12

Df(2L)VA13

(Wright, 1996, Wright et al., 1981, Gilbert et al., 1984)

Df(2L)VA17

(Pentz et al., 1986, Wright, 1996, Wright et al., 1981, Stathakis et al., 1995, Gilbert et al., 1984)

Df(2L)VA19

(Stathakis et al., 1995, Wright, 1996)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UAS-Acn.2xMyc}

characterization construct

P{Acn.4.2.Myc}

P{Acn.+t4.2}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}l(2)SH0843^SH0843

insertion of mobile activating element

P{EPgy2}Acn^EY22500

P{GSV1}EP-323^EP-323

P{GSV2}CG10470^GS5198

Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )

Terms Based on Experimental Evidence ( 6 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

protein targeting to lysosome

References

inferred from mutant phenotype

(Haberman and Krämer, 2007)

regulation of autophagy

inferred from mutant phenotype

Cellular Component

CV term

catalytic step 2 spliceosome

References

inferred from direct assay

(Herold et al., 2009)

cytoplasm

inferred from direct assay

nucleus

inferred from direct assay

precatalytic spliceosome

inferred from direct assay

(Herold et al., 2009)

Terms Based on Predictions or Assertions ( 4 terms )

Molecular Function

CV term

(FlyBase Curators et al., 2004-)

References

nucleotide binding

inferred from electronic annotation with InterPro:IPR012677

Biological Process

CV term

nuclear mRNA splicing, via spliceosome

References

apoptotic process

inferred from sequence or structural similarity

(Vernooy et al., 2000)

inferred by curator from GO:0071011|GO:0071013

(Herold et al., 2009)

Cellular Component

CV term

References

nucleus

inferred from sequence or structural similarity

(Vernooy et al., 2000)

Sequence Ontology: Class of Gene

gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Protein-protein

Interacting group

Assay

References

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Acn allele

Gene

References

External Data

DroID - A comprehensive database of gene and protein interactions.

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FBgn0263198

Orthologs

Genome-wide drosophilid orthologs

Curated drosophilid orthologs

Dpse\GA10336

Dyak\GE13225

y¹ w^67c23; P{EPgy2}Acn^EY22500

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

Bloomington

6102

Acn^fs1/CyO

3388

Acn¹ pr¹/CyO

22565

VDRC

v102407

P{KK111555}VIE-260B

v16052

w¹¹¹⁸; P{GD6985}v16052/TM3

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 150 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

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Antibody Information

polyclonal

Other Information

Discoverer

Etymology

The gene is named based on its homology to mammalian "Acinus".

(Haberman and Krämer, 2007)

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: hkl CG10473

(Haberman, 2007.2.26)

Source for database merge of

Source for merge of: l(2)37Ba CG10473

(FlyBase, 1996-)

Additional comments

Other Comments

Mutants show a mild reduction in eye colour and a reduction in trafficking of proteins to lysosomes.

Located between coordinates -72.3 and -68.1.

(Thor and Thomas, 1997, Wright, 1996, McCrady and Tolin, 1994)

Variably late lethal; death occurs at larval ^{l(2)37Ba<up>2}</up>, or pupal <up>l(2)37Ba², l(2)37Ba⁴, l(2)37Ba⁸, l(2)37Ba⁹</up> stage with pharate adults; l(2)37Ba² produces some adults, which are male sterile or female sterile; l(2)37Ba⁴ produces many adults, which are male or female sterile; l(2)37Ba^fs1 is a normally viable, female-sterile allele. Melanotic tumors noted in l(2)37Ba⁴ and l(2)37Ba⁸; pharate adults display incompletely developed abdominal cuticle in l(2)37Ba², l(2)37Ba⁴ and l(2)37Ba⁸ (Wright, 1987).

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

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RefSeq (Proteins)

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DDBJ / EMBL / GenBank

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Entrez Gene - A searchable database of RefSeq genes.

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35173

UniProtKB/TrEMBL

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Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Nucleotide-binding, alpha-beta plait (IPR012677)

Linkouts

DroID - A comprehensive database of gene and protein interactions.

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FBgn0263198

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

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Synonyms & Secondary IDs ( 14 )

Reported As

Symbol Synonym

Acn

CG10437

(Vernooy et al., 2000)

CG10473

(Haberman, 2007.2.26, Laviolette et al., 2005, Molnar et al., 2006, Cakouros et al., 2008, Keleman et al., 2009.8.5, Herold et al., 2009)

dAcn

dacn

(Haberman, 2007.2.26, Haberman and Krämer, 2007, Haberman et al., 2008, Herold et al., 2009)

hkl

l(2)37Ba

(McCrady and Tolin, 1994, FlyBase, 1996-, Wright, 1987)

Name Synonym

Acinus

acinus

CG10473

Drosophlia homolog of acinus