FlyBase Gene Report: Dmel\Cdk7

General Information

Symbol

Dmel\Cdk7

Species

D. melanogaster

Name

Cyclin-dependent kinase 7

Annotation Symbol

CG3319

Feature Type

protein_coding_gene

FlyBase ID

FBgn0263237

Gene Model Status

Current

Stock Availability

18 publicly available

Enzyme Name (EC)

cyclin-dependent kinase (2.7.11.22)

[RNA-polymerase]-subunit kinase (2.7.11.23)

Gene Summary

Cyclin-dependent kinase 7 (Cdk7) encodes a serine/threonine protein kinase whose activity depends on the regulatory subunits encoded by CycH and Mat1. This trimeric Cdk complex activates other Cdks by phosphorylation in their T-loop and is called Cdk activating kinase (CAK). CAK can also be part of TFIIH, where the product of Cdk7 phosphorylates different transcription substrates (e.g. CTD of Pol II). [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

Mo15, DmMO15, DmCdk7

Key Links

Genomic Location

Cytogenetic map

4F4-4F4

Sequence location

X:5,307,465..5,308,983 [-]

Recombination map

1-11

RefSeq locus

NC_004354 REGION:5307465..5308983

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables cyclin-dependent protein serine/threonine kinase activity

inferred from direct assay

(Larochelle et al., 2001)

enables protein serine/threonine kinase activity

inferred from direct assay

(Cho et al., 2020, Larochelle et al., 1998)

enables RNA polymerase II CTD heptapeptide repeat kinase activity

inferred from direct assay

(Schwartz et al., 2003, Larochelle et al., 2001)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(InterPro Project Members, 2004-)

enables cyclin-dependent protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000624096

(GO Reference Genome Project, 2011-)

enables RNA polymerase II CTD heptapeptide repeat kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000624096

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR037770

(InterPro Project Members, 2004-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in mitotic cell cycle

inferred from mutant phenotype

(Larochelle et al., 1998)

involved_in negative regulation of hippo signaling

inferred from genetic interaction with FLYBASE:yki; FB:FBgn0034970

(Cho et al., 2020)

involved_in programmed cell death

inferred from mutant phenotype

(Morishita et al., 2013)

involved_in RNA polymerase II promoter clearance

inferred from direct assay

(Schwartz et al., 2003)

involved_in transcription by RNA polymerase II

inferred from direct assay

(Schwartz et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in positive regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000624096

(GO Reference Genome Project, 2011-)

involved_in regulation of cell cycle

inferred from biological aspect of ancestor with PANTHER:PTN000624096

(GO Reference Genome Project, 2011-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

part_of carboxy-terminal domain protein kinase complex

inferred from direct assay

(Larochelle et al., 2001)

located_in cytoplasm

inferred from direct assay

(Chen et al., 2003)

located_in nucleolus

inferred from direct assay

(Liu et al., 2017)

located_in nucleoplasm

inferred from direct assay

(Liu et al., 2017)

located_in nucleus

inferred from direct assay

(Chen et al., 2003)

part_of transcription factor TFIIH holo complex

inferred from direct assay

(Aguilar-Fuentes et al., 2008, Fregoso et al., 2007)

inferred from physical interaction with FLYBASE:hay; FB:FBgn0001179,FLYBASE:CycH; FB:FBgn0022936,FLYBASE:Mat1; FB:FBgn0024956,FLYBASE:mrn; FB:FBgn0261109,FLYBASE:Xpd; FB:FBgn0261850

(Stettler et al., 2015)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000624096

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000623091

(GO Reference Genome Project, 2011-)

part_of transcription factor TFIIH holo complex

inferred from electronic annotation with InterPro:IPR037770

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with HGNC:1778

(Aoyagi and Wassarman, 2000)

inferred from sequence or structural similarity with SGD:S000002266

(Aoyagi and Wassarman, 2000)

part_of transcription factor TFIIK complex

inferred from electronic annotation with InterPro:IPR037770

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000624096

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CYCLIN DEPENDENT KINASES

CDK-ACTIVATING KINASE COMPLEX

CDK-ACTIVATING KINASE-XERODERMA PIGMENTOSUM D COMPLEX

Pathway (FlyBase)

Negative Regulators of Hippo Signaling Pathway

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

cyclin-dependent protein serine/threonine kinase activity

(1) L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+) (2.7.11.22)

(2) L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+) (2.7.11.22)

RNA polymerase II CTD heptapeptide repeat kinase activity

[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+) (2.7.11.23)

RHEA 10216:

Summaries

Gene Snapshot

Gene Group (FlyBase)

CYCLIN DEPENDENT KINASES -: Cyclin-dependent kinases (CDKs) are serine/threonine kinases whose activity depends on a regulatory cyclin subunit. (Adapted from PMID:25180339).
CDK-ACTIVATING KINASE COMPLEX -: The cyclin-dependent kinase (CDK)-activating kinase (CAK) complex is a three-subunit kinase module present as a subcomplex of TFIIH, as free trimeric CAK or in complex with the ATP-dependent DNA helicase XPD (Xpd in D.mel). The TFIIH-associated CAK complex phosphorylates the C-terminal domain of RNA pol II. The free form of CAK activates the cell cycle CDKs. The CAK-XPD complex regulates the cell cycle progression in mitosis. (Adapted from FBrf0227236 and PMID:21592869).
CDK-ACTIVATING KINASE-XERODERMA PIGMENTOSUM D COMPLEX -: The cyclin-dependent kinase (CDK)-activating kinase-Xeroderma pigmentosum D (CAK-XPD) complex is a four-subunit kinase module. CAK-XPD has a role in the regulation of the cell cycle progression in mitosis, where XPD negatively regulates the cell cycle function of the CAK activity. (Adapted from FBrf0227236, FBrf0211947, FBrf0159708 and PMID:21592869).

Pathway (FlyBase)

Negative Regulators of Hippo Signaling Pathway -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Summary (Interactive Fly)

a Cdk-activating kinase and general transcription factor - activity depends on the regulatory CycH and Mat1 subunits - this trimeric Cdk complex activates other Cdks by phosphorylation in their T-loop and is called Cdk activating kinase (CAK) - CAK can be part of TFIIH, where Cdk7 phosphorylates different transcription substrates (e.g. CTD of Pol II)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Cdk7 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q24216)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Cdk7-RA

FBtr0070756

NM_078489

1448

353

Additional Transcript Data and Comments

Reported size (kB)

1.6 (northern blot)

(Larochelle et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Cdk7-PA

FBpp0070723

39.7

353

9.98

Q24216

NP_511044

AAF46016

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

353 (aa); 40 (kD observed); 39 (kD predicted)

(Larochelle et al., 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q24216

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cdk7 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cdk7_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.20

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 2

organism | faint | ubiquitous | uniform

Comment: maternally deposited

(Leclerc et al., 2000)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic cycle 9 -- embryonic cycle 11

organism | ubiquitous | uniform

(Leclerc et al., 2000)

embryonic stage 4 -- 6

central brain anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal epidermis primordium

Comment: reported as dorsal epidermis anlage

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11

organism | faint

(Leclerc et al., 2000)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

ventral epidermis primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Larochelle et al., 1998)

adult stage | female

ovary

(Larochelle et al., 1998)

Additional Descriptive Data

Cdk7 transcripts are present throughout development with the highest levels in early embryos and ovaries.

(Larochelle et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 11

organism

(Leclerc et al., 2000)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of carboxy-terminal domain protein kinase complex

inferred from direct assay

(Larochelle et al., 2001)

located_in cytoplasm

inferred from direct assay

(Chen et al., 2003)

located_in nucleolus

inferred from direct assay

(Liu et al., 2017)

located_in nucleoplasm

inferred from direct assay

(Liu et al., 2017)

located_in nucleus

inferred from direct assay

(Chen et al., 2003)

part_of transcription factor TFIIH holo complex

inferred from direct assay

(Aguilar-Fuentes et al., 2008, Fregoso et al., 2007)

inferred from physical interaction with FLYBASE:hay; FB:FBgn0001179,FLYBASE:CycH; FB:FBgn0022936,FLYBASE:Mat1; FB:FBgn0024956,FLYBASE:mrn; FB:FBgn0261109,FLYBASE:Xpd; FB:FBgn0261850

(Stettler et al., 2015)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Cdk7 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cdk7

View exonic expression by tissue for Dmel\Cdk7

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263237

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31441/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263237

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015617

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263237

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 25 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cdk7

Transgenic constructs containing regulatory region of Cdk7

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 12 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Cdk7^GD4167

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Cdk7^GD4167

viable, with Scer\GAL4^Mef2.PR

Cdk7^GD4167

viable, with Scer\GAL4^tin.CΔ4

Cdk7^GD4167

Sterility

Allele

female sterile, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

Cdk7^GL00073

Other Phenotypes

Allele

decreased cell death, with Scer\GAL4^GMR.PF

Cdk7^GD4167

decreased cell death | temperature conditional

Cdk7^S164A.T170A

decreased occurrence of cell division | larval stage

Cdk7^P140S

decreased occurrence of cell division | oogenesis

Cdk7^ts

some die during pupal stage, with Scer\GAL4^pnr-MD237

Cdk7^GD4167

visible

Cdk7^P140S

visible, with Scer\GAL4^Bx-MS1096

visible, with Scer\GAL4^pnr-MD237

Cdk7^GD4167

wild-type

wild-type, with Scer\GAL4^Bx-MS1096

Cdk7^UAS.Tag:HA

Phenotype manifest in

Allele

chaeta

Cdk7^P140S

cuticle

Cdk7^P140S

embryo

Cdk7^{nanos-D137R.bcd3'UTR}

embryo, with Scer\GAL4^Kr.PM

female germline cell | temperature conditional

Cdk7^ts

mitotic cell cycle | anterior | embryonic stage

Cdk7^{nanos-D137R.bcd3'UTR}

mitotic cell cycle | embryonic cycle 14

Cdk7^{nanos-D137R.bcd3'UTR}

mitotic cell cycle | embryonic stage 1, with Scer\GAL4^Kr.PM

somatic cell of ovariole | temperature conditional

Cdk7^ts

trichogen cell, with Scer\GAL4^pnr-MD237

Cdk7^GD4167

wing

Cdk7^P140S

wing, with Scer\GAL4^Bx-MS1096

wing vein, with Scer\GAL4^Bx-MS1096

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (44)

Hsap\CDK7

13 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (23)

Rnor\Cdk7

12 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (26)

Mmus\Cdk7

12 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (23)

Xtro\cdk7

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (24)

Drer\cdk7

13 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (28)

Cele\cdk-7

14 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (18)

Agam\AgaP_AGAP002646

12 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (63)

Atha\CAK4

12 of 13

Yes

Atha\CDKD1;1

11 of 13

Yes

Atha\CDKD1;3

11 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (16)

Scer\KIN28

12 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (13)

Spom\mcs6

11 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Cdk7. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (21)

5 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

nmo

1 of 13

p38a

1 of 13

p38b

1 of 13

sgg

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CDK7; cyclin dependent kinase 7

13 of 14

601955

CDK20; cyclin dependent kinase 20

3 of 14

610076

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Cdk7 - CycH

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Rhee et al., 2014, Guruharsha et al., 2011)

Cdk7 - Mat1

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, experimental knowledge based

(Chlamydas et al., 2016, Guruharsha et al., 2011, Aguilar-Fuentes et al., 2006)

Cdk7 - Ssl1

anti bait coimmunoprecipitation, western blot

(Aguilar-Fuentes et al., 2008)

Cdk7 - Xpd

anti bait coimmunoprecipitation, western blot

(Nag et al., 2018, Aguilar-Fuentes et al., 2006)

Cdk7 - hay

anti bait coimmunoprecipitation, western blot

(Aguilar-Fuentes et al., 2006)

Cdk7 - msl-1

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation

(Chlamydas et al., 2016)

Cdk7 - sim

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Cdk7 - yki

enzymatic study, western blot

(Cho et al., 2020)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Cdk7

enhanceable

hay

(Merino et al., 2002)

Cdk7

enhanceable

twe

(Leclerc et al., 2000)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

hid

suppressible

Cdk7

(Morishita et al., 2013)

suppressible

(Nag et al., 2018)

suppressible

(Morishita et al., 2013)

rpr

suppressible

Cdk7

(Morishita et al., 2013)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0263237

MIST (genetic) - An integrated Molecular Interaction Database

31441

MIST (protein-protein) - An integrated Molecular Interaction Database

31441

Pathways

Signaling Pathways (FlyBase)

Negative Regulators of Hippo Signaling Pathway

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24216

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-11

Cytogenetic map

4F4-4F4

Sequence location

X:5,307,465..5,308,983 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

4F4-4F4

Limits computationally determined from genome sequence between P{EP}EP425 and P{EP}CG3249^EP1400

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-11

(FlyBase, 2016)

1-13.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (18)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

98097

y¹ w^*; P{Cdk7.S164A.T170A}3

62304

y¹ v¹; P{TRiP.HMJ23661}attP40/CyO

4557

Df(1)JB254, P{snf⁺,dhd⁺}SL2, w^*/FM7c

35199

y¹ sc^* v¹ sev²¹; P{TRiP.GL00073}attP2

Kyoto

107796

Df(1)JB254, P{snf⁺,dhd⁺}SL2, w^*/FM7c

107810

w^*; P{Cdk7^P140S}SL1 Sb¹/TM3, Ser¹

NIG-Fly

3319R-1

P{NIG.3319R}1

3319R-2

P{NIG.3319R}2

HMJ23661

y¹ v¹; P{TRiP.HMJ23661}attP40/CyO

VDRC

v103413

P{KK100572}VIE-260B

v10442

w¹¹¹⁸; P{GD4167}v10442

v342048

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01435}attP40/CyO-GFP

More stocks available...

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (11)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP01401

Other clones

DPiM_IP01401

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0263237

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Kachaev et al., 2019, Leclerc et al., 2000)

monoclonal

(Larochelle et al., 1998)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

20H5

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The Cdk7 complex regulates the mitochondrial localization of W and its ability to induce apoptosis.

(Morishita et al., 2013)

DNA-protein interactions: genome-wide binding profile assayed for Cdk7 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).

(Filion et al., 2010)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Cdk7 probably has a role in the control of the transcriptional machinery.

(Leclerc et al., 2000)

Cdk7 is required for mitosis and for the activation of cdc2 protein in vivo.

(Larochelle et al., 1998)

Cdk7 is involved in transcriptional control in the embryo and in the larvae.

(Leopold and Leclerc, 1997)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Cdk7 CG3319

(Li, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CD 7

(Santoso and Kadonaga, 2006)

CDK7

(Cho and Jiang, 2021, Morishita et al., 2013, Herrera-Cruz et al., 2012, Filion et al., 2010, Larochelle et al., 2001)

CG3319

(Widmer et al., 2018, Hosono et al., 2015, Stettler et al., 2015, Haelterman et al., 2014, Ni et al., 2011.7.19, Johnson et al., 2010, Ni et al., 2010.12.1, Ardehali et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Aoyagi and Wassarman, 2000, Morrison et al., 2000)

DmCDK7

(Aguilar-Fuentes et al., 2006, Leopold and Leclerc, 1997)

DmCdk7

(Harper and Elledge, 1998, Leclerc et al., 1996)

DmMO15

(Larochelle, 1996.4.24)

DmMo15

(Hager and Cline, 1997)

Dmcdk7

(Larochelle et al., 1998)

Mo15

cdk-7

(Deshpande et al., 2007)

cdk7

(Zurita and Murillo-Maldonado, 2020, Cruz-Becerra et al., 2016, Johnson et al., 2010, Emberly et al., 2008, Fregoso et al., 2007, Matsuno et al., 2007, Fisher, 2005, Ito et al., 2004, Schwartz et al., 2003, Merino et al., 2002, Larochelle et al., 2001, Merino et al., 2001, Leclerc et al., 2000)

dCdk7

(Ito et al., 2004)

p40^MO15

(Kaldis, 1999)

Name Synonyms

Cyclin-dependent kinase 7

(Graves et al., 2020)

Mo15

cyclin-dependent kinase 7

(Fisher, 2005)

Secondary FlyBase IDs

FBgn0015617
FBgn0024324

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

transgene_used

FBGSE22069

genome binding

Protein profiling reveals five principal chromatin types in Drosophila cells.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 57 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31441

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

U56661

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q24216

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q24216

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q24216

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263237

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31441/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263237

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263237

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3319

MARRVEL_MODEL - MARRVEL (model organism gene)

31441

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0263237

DroID - A comprehensive database of gene and protein interactions.

FBgn0263237

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

20H5

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cdk7_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015617

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0263237

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0263237

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC003871

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

31441

MIST (protein-protein) - An integrated Molecular Interaction Database

31441

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24216

References (111)

The following external sites may use different assemblies or annotations than FlyBase.

0.20

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