General Information
Symbol
Dmel\Cdk7
Species
D. melanogaster
Name
Cyclin-dependent kinase 7
Annotation Symbol
CG3319
Feature Type
FlyBase ID
FBgn0263237
Gene Model Status
Stock Availability
Enzyme Name (EC)
Cyclin-dependent kinase (2.7.11.22)
[RNA-polymerase]-subunit kinase (2.7.11.23)
Gene Snapshot
Cyclin-dependent kinase 7 (Cdk) is a serine/threonine protein kinase whose activity depends on the regulatory CycH and Mat1 subunits. This trimeric Cdk complex activates other Cdks by phosphorylation in their T-loop and is called Cdk activating kinase (CAK). CAK can also be part of TFIIH, where Cdk7 phosphorylates different transcription substrates (e.g. CTD of Pol II). [Date last reviewed: 2016-08-25]
Also Known As
DmCdk7, DmMO15
Genomic Location
Cytogenetic map
Sequence location
X:5,307,465..5,308,983 [-]
Recombination map
1-11
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Protein Family (UniProt, Sequence Similarities)
-
Catalytic Activity (EC)
Experimental Evidence
ATP + a protein = ADP + a phosphoprotein (2.7.11.22)
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate (2.7.11.23)
Predictions / Assertions
ATP + a protein = ADP + a phosphoprotein (2.7.11.22)
Summaries
Gene Group Membership
CYCLIN DEPENDENT KINASES -
Cyclin-dependent kinases (CDKs) are serine/threonine kinases whose activity depends on a regulatory cyclin subunit. (Adapted from PMID:25180339).
CDK-ACTIVATING KINASE COMPLEX -
The cyclin-dependent kinase (CDK)-activating kinase (CAK) complex is a three-subunit kinase module present as a subcomplex of TFIIH, as free trimeric CAK or in complex with the ATP-dependent DNA helicase XPD (Xpd in D.mel). The TFIIH-associated CAK complex phosphorylates the C-terminal domain of RNA pol II. The free form of CAK activates the cell cycle CDKs. The CAK-XPD complex regulates the cell cycle progression in mitosis. (Adapted from FBrf0227236 and PMID:21592869).
CDK-ACTIVATING KINASE-XERODERMA PIGMENTOSUM D COMPLEX -
The cyclin-dependent kinase (CDK)-activating kinase-Xeroderma pigmentosum D (CAK-XPD) complex is a four-subunit kinase module. CAK-XPD has a role in the regulation of the cell cycle progression in mitosis, where XPD negatively regulates the cell cycle function of the CAK activity. (Adapted from FBrf0227236, FBrf0211947, FBrf0159708 and PMID:21592869).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Cdk7 or the JBrowse view of Dmel\Cdk7 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.50
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0070756
1448
353
Additional Transcript Data and Comments
Reported size (kB)
1.6 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0070723
39.7
353
9.98
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
353 (aa); 40 (kD observed); 39 (kD predicted)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cdk7 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (18 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000623091
(assigned by GO_Central )
Biological Process (7 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000624096
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000623091
(assigned by GO_Central )
Cellular Component (7 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000624096
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000623091
(assigned by GO_Central )
inferred from sequence or structural similarity with HGNC:1778
inferred from sequence or structural similarity with SGD:S000002266
inferred from biological aspect of ancestor with PANTHER:PTN000624096
(assigned by GO_Central )
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

dorsal ectoderm anlage

Comment: anlage in statu nascendi

ventral ectoderm anlage

Comment: anlage in statu nascendi

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

antennal anlage

Comment: reported as procephalic ectoderm anlage

central brain anlage

Comment: reported as procephalic ectoderm anlage

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

visual anlage

Comment: reported as procephalic ectoderm anlage

antennal primordium

Comment: reported as procephalic ectoderm primordium

central brain primordium

Comment: reported as procephalic ectoderm primordium

visual primordium

Comment: reported as procephalic ectoderm primordium

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

dorsal epidermis primordium

Comment: reported as dorsal epidermis anlage

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Cdk7 transcripts are present throughout development with the highest levels in early embryos and ovaries.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Cdk7 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Allele of Cdk7
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    0
    --
    Other relevant insertions
    Transgenic Constructs ( 19 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Cdk7
    Allele of Cdk7
    Mutagen
    Associated Transgenic Construct
    Stocks
    Transgenic constructs containing regulatory region of Cdk7
    Deletions and Duplications ( 0 )
    Summary of Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Sterility
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (7)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    14 of 15
    Yes
    Yes
    3 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
     
    1 of 15
    No
    No
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (6)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    14 of 15
    Yes
    Yes
    2 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    Rattus norvegicus (Norway rat) (6)
    12 of 13
    Yes
    Yes
    3 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    1 of 13
    No
    No
    Xenopus tropicalis (Western clawed frog) (5)
    12 of 12
    Yes
    Yes
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    Danio rerio (Zebrafish) (6)
    14 of 15
    Yes
    Yes
    3 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    Caenorhabditis elegans (Nematode, roundworm) (5)
    12 of 15
    Yes
    Yes
    3 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    Arabidopsis thaliana (thale-cress) (3)
    9 of 9
    Yes
    Yes
    8 of 9
    No
    Yes
    8 of 9
    No
    Yes
    Saccharomyces cerevisiae (Brewer's yeast) (5)
    14 of 15
    Yes
    Yes
    1 of 15
    No
    Yes
    1 of 15
    No
    No
    1 of 15
    No
    No
    1 of 15
    No
    No
    Schizosaccharomyces pombe (Fission yeast) (4)
    12 of 12
    Yes
    Yes
    1 of 12
    No
    No
    1 of 12
    No
    No
    1 of 12
    No
    No
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190B2S )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506GB )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Glossina morsitans
    Tsetse fly
    Lucilia cuprina
    Australian sheep blowfly
    Mayetiola destructor
    Hessian fly
    Aedes aegypti
    Yellow fever mosquito
    Anopheles darlingi
    American malaria mosquito
    Anopheles gambiae
    Malaria mosquito
    Culex quinquefasciatus
    Southern house mosquito
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06IU )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Bombyx mori
    Silkmoth
    Danaus plexippus
    Monarch butterfly
    Heliconius melpomene
    Postman butterfly
    Apis florea
    Little honeybee
    Apis mellifera
    Western honey bee
    Bombus impatiens
    Common eastern bumble bee
    Bombus terrestris
    Buff-tailed bumblebee
    Linepithema humile
    Argentine ant
    Megachile rotundata
    Alfalfa leafcutting bee
    Nasonia vitripennis
    Parasitic wasp
    Dendroctonus ponderosae
    Mountain pine beetle
    Tribolium castaneum
    Red flour beetle
    Pediculus humanus
    Human body louse
    Rhodnius prolixus
    Kissing bug
    Cimex lectularius
    Bed bug
    Acyrthosiphon pisum
    Pea aphid
    Zootermopsis nevadensis
    Nevada dampwood termite
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06EM )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strigamia maritima
    European centipede
    Ixodes scapularis
    Black-legged tick
    Tetranychus urticae
    Two-spotted spider mite
    Daphnia pulex
    Water flea
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0CI9 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strongylocentrotus purpuratus
    Purple sea urchin
    Strongylocentrotus purpuratus
    Purple sea urchin
    Ciona intestinalis
    Vase tunicate
    Ciona intestinalis
    Vase tunicate
    Gallus gallus
    Domestic chicken
    Human Disease Model Data
    FlyBase Human Disease Model Reports
      Alleles Reported to Model Human Disease (Disease Ontology)
      Download
      Models ( 0 )
      Allele
      Disease
      Evidence
      References
      Interactions ( 0 )
      Allele
      Disease
      Interaction
      References
      Comments ( 0 )
       
      Human Orthologs (via DIOPT v7.1)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      Pathways
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      X
      Recombination map
      1-11
      Cytogenetic map
      Sequence location
      X:5,307,465..5,308,983 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      4F4-4F4
      Limits computationally determined from genome sequence between P{EP}EP425 and P{EP}CG3249EP1400
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Experimentally Determined Recombination Data
      Location
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (11)
      Genomic Clones (11)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (11)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        RNAi and Array Information
        Linkouts
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
        Commercially Available Antibodies
         
        Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for identity of: Cdk7 CG3319
        Source for database merge of
        Additional comments
        Other Comments
        The Cdk7 complex regulates the mitochondrial localization of W and its ability to induce apoptosis.
        DNA-protein interactions: genome-wide binding profile assayed for Cdk7 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).
        dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
        Cdk7 probably has a role in the control of the transcriptional machinery.
        Cdk7 is required for mitosis and for the activation of cdc2 protein in vivo.
        Cdk7 is involved in transcriptional control in the embryo and in the larvae.
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 45 )
        Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        Linkouts
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        KEGG Genes - Molecular building blocks of life in the genomic space.
        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
        Synonyms and Secondary IDs (16)
        Reported As
        Name Synonyms
        Cyclin-dependent kinase 7
        Mo15
        cyclin-dependent kinase 7
        Secondary FlyBase IDs
        • FBgn0015617
        • FBgn0024324
        Datasets (1)
        Study focus (1)
        Experimental Role
        Project
        Project Type
        Title
        • transgene_used
        Protein profiling reveals five principal chromatin types in Drosophila cells.
        References (91)