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Gene Dmel\AP-50

General Information
Symbol	Dmel\AP-50	Species	D. melanogaster
Name	AP-50	Annotation symbol	CG7057
Feature type	protein_coding_gene	FlyBase ID	FBgn0263351
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	AP50, μ2
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	94A15-94A16	Sequence location	3R:18,288,153..18,290,325 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene AP-50 is referred to in FlyBase by the symbol Dmel\AP-50 (CG7057, FBgn0263351). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: dsRNA transport; endocytosis. 7 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: AP complex, mu/sigma subunit; Clathrin adaptor, mu subunit; Clathrin adaptor, mu subunit, C-terminal; Clathrin adaptor, mu subunit, conserved site; Clathrin coat associated protein AP-50; Longin-like. Gene sequence location is 3R:18288153..18290325.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References Yang et al., 2011, Neuron 72(2): 285--299 Sequence features BKN20496 HFA14090
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 94A15-94A16   Limits computationally determined from genome sequence between P{PZ}l(3)rQ178rQ178 and P{lacW}Dph5L4910
Experimentally Determined Cytological Location
Cytogenetic map Notes References 94B-94B   Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000) 94B-94B     (Zhang, 1998.5.6) 94B1-94B2     (Mullins, 1998.12.1) 94B1-94B5   (determined by in situ hybridisation)   (Zhang and Broadie, 1999)
Experimentally Determined Recombination Data
Location	3- (Menut et al., 2007)
Left of (cM)
Right of (cM)
Notes	Maps to 3R. (Menut et al., 2007)
Gene Model & Products
Please see the GBrowse view of Dmel\AP-50 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) AP-50-RA FBtr0084252  NM_170015   1980   437 AP-50-RB FBtr0084253  NM_142792   2049   437 AP-50-RC FBtr0301866  NM_001170215   2000   437
Additional Transcript Data & Comments
Reported size (kB)	1.866 (sequence analysis) (Mullins et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein AP-50-PA   FBpp0083645   49.9   437   10.03     NP_732744   AAF56001 AP-50-PB   FBpp0083646   49.9   437   10.03     NP_651049   AAF56002 AP-50-PC   FBpp0291080   49.9   437   10.03     NP_001163686   ACZ94982
Additional Polypeptide Data & Comments
Reported size (kDa)	437 (aa); 50 (kD predicted) (Zhang and Broadie, 1999) 437 (aa) (Mullins et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Clathrin adaptor, mu subunit (IPR001392) Clathrin adaptor, mu subunit, C-terminal (IPR008968) Longin-like (IPR011012) Clathrin adaptor, mu subunit, conserved site (IPR018240) Clathrin coat associated protein AP-50 (IPR015629) AP complex, mu/sigma subunit (IPR022775)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141728   AA141729   AA567297   AA696461   AA696780   AA816863   AE014297 AAF56001   AE014297 AAF56002   AE014297 ACZ94982   AF083300   AF110232 AAF14248   AI516565   AI533385   AI533540   AI534246   AJ005962 CAA06785   AW944413   AX260142   AY070712 AAL48183   BF488492   BI164901   BI239602   BI240001   BI485461   BI563621   BI566837   BI574977   BI576416   BI576581   BI587895   BI588755   BI588790   BI605000   BI637329   BI638992   BI639670   BI641050   CO153552   CO267214   CO271196   CO273686   CO274872   CO285479   CO286476   CO311317   CO321187   EC062654   EC072716   EC197473   EC206724   EC210331   EC232520   EC236525   EC250574   EL876864   EV575613   GH748872   GH750326   GH761396   GH771490   GH771758   GH772008   GH772231   GH772810   GH776013   GH776197   GH776643   GH777390   GH783222   GH787990   GH789213   GH791178   GH800147   GH801031   GH803719   GH805337   GH809023   GH809507   GH809511   GH811698   GH812867   GH814051   GH815907   GH817849   GH818435   GH818949   GH819862   GH821357   GH823793   GH825887   GH832535   GH833932   GH836380   GH837996   GH838002   GH839693   GH842226   GH845435   GH848468   GH848733   GH848841   GH849107   GH850522   GH854612   GH855077   GH860283   GH862422   GH866229   GH868045   GH868451   GH871501   GH872649   GH884113   GH894284   GH895667   GH898568   GH903134   GH903334   GH905180   GH905714   GH910487   GH912584   GH913273   GH917857   GH931407   GH932997   GH934673   GH935481   GH937540   GH939438   GH940914   GH945755   GH973807
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O62530
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 9 -- 17   anterior embryonic/larval midgut   (Zhang and Broadie, 1999) posterior embryonic/larval midgut   (Zhang and Broadie, 1999) embryonic neuroblast   (Zhang and Broadie, 1999) embryonic stage 13   gastric caecum   (Zhang and Broadie, 1999) embryonic stage 13 -- 17   embryonic/larval midgut   (Zhang and Broadie, 1999) presumptive embryonic/larval central nervous system   (Zhang and Broadie, 1999) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Mullins et al., 1999)
Additional Descriptive Data
	AP-50 transcripts are detected at all stages of development tested. (Mullins et al., 1999) AP-50 transcripts are first detected in stage 9 embryos in the midgut and in the CNS. By stage 13, staining in both the midgut and CNS is strong and persists into later embryonic stages. (Zhang and Broadie, 1999)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. [an error occurred while processing this directive] modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_30
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4pnr-MD237 AP-50GD12117 Phenotype manifest in Allele eye disc | somatic clone AP-50NN20
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of AP-50 Class Mutagen Stocks Known lesion AP-50G4842 P-element activity 1 -- AP-50G7085 P-element activity 1 -- AP-50NN20 loss of function allele ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of AP-50 Class Mutagen Stocks Known lesion AP-50GD12117 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes AP-50JF02875 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AP-50KK100400 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AP-50Act5C.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6191 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12117} NA P{KK100400} NA P{TRiP.JF02875} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}AP-50G4842 NA P{EP}AP-50G7085 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
dsRNA transport inferred from mutant phenotype (Saleh et al., 2006) endocytosis inferred from mutant phenotype (Windler and Bilder, 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
intracellular protein transport inferred from electronic annotation with InterPro:IPR001392, InterPro:IPR008968, InterPro:IPR015629, InterPro:IPR018240 (FlyBase Curators et al., 2004-) neurotransmitter secretion non-traceable author statement (Stimson and Ramaswami, 1999) synaptic vesicle coating traceable author statement (Stimson and Ramaswami, 1999) vesicle coating traceable author statement (Stimson and Ramaswami, 1999)
Cellular Component
CV term Evidence References
clathrin adaptor complex inferred from electronic annotation with InterPro:IPR001392, InterPro:IPR008968, InterPro:IPR015629, InterPro:IPR018240 (FlyBase Curators et al., 2004-) clathrin coat of coated pit inferred from electronic annotation with InterPro:IPR015629 (FlyBase Curators et al., 2004-) coated vesicle traceable author statement (Stimson and Ramaswami, 1999) synaptic vesicle traceable author statement (Stimson and Ramaswami, 1999)
Sequence Ontology: Class of Gene
	gene (Menut et al., 2007) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions AP-50 allele Gene References
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23377 Dere\AP-50 Dgri\GH22222 Dmoj\GI21976 Dper\GL24218 Dpse\GA20066 Dsec\GM26435 Dsim\AP-50 Dvir\GJ14336 Dwil\GK11695 Dyak\AP-50
Curated drosophilid orthologs	Dere\AP-50 (Akashi et al., 2005.8.16) Dore\AP-50 (Akashi et al., 2005.8.16) Dore\boss (Akashi et al., 2005.8.16) Dpse\GA20066   Dsan\AP-50 (Carbone et al., 2005, Llopart et al., 2005) Dsim\AP-50 (Begun, 2000.4.5) Dtei\AP-50 (Akashi et al., 2005.8.16) Dyak\AP-50 (Begun, 2000.4.7)
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	30102 w1118; P{EP}AP-50G4842/TM6C, Sb1 32610 w1118; P{EP}AP-50G7085/TM6C, Sb1 28040 y1 v1; P{TRiP.JF02875}attP2/TM3, Sb1
VDRC	v103390 P{KK100400}VIE-260B v27820 w1118; P{GD12117}v27820 v27821 w1118; P{GD12117}v27821/CyO
Genomic Clones ( 2 )
	BACR30J14 BACR48C17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 123 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD05403
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT23781 GM02287 GM07724 GM08382 LD20043 LD42466 RE04668 RE36145 RE36641 RE68785 RH27533 RH28803 RH30170 RH30219 RH32971 RH33171 RH34289 RH36589 RH70512 SD05175 SD07011 SD19311 SD21413 SD22316 SD24141
Other clones	11397 19269 34307 56726 97783 180994 CK01.50A.B2 CK01.142C.F3 CK02342 Dmel_dig_003120_0617_1947 EK062928 EK102307 EK111916 EK115822 EK149124 EK169055 EK224132 EK290451 EN02333 IP06769 RP003012859 RP003040319 RP003044189 RP003053318 RP003057095 RP003061260 RP003064943 RP003066944 RP003068608 RP003068640 RP003071209 RP003073776 RP003074107 RP003079543 RP003082970 RP003087163 RP003096348 RP003109740 RP003112787 RP003134778 RP003137823 RP003143624 RP003166029 RP003169884 RP003173726 RP003174535 RP003182923 RP003192822 RP003195584 RP003200415 RP003205779 RP003206829 RP003209082 RP003211547 RP003214615 RP003223094 RP003233102 RP003239199 RP003242270 RP003270497 RP003273277 RP003282550 RP003290747 RP003305313 RP003338889 RP003386728 RP003398240 RP003425424 RP003449144 RP003462618 RP003465025 RP003465397 RP003479082 RP003490170 RP003494016 RP003494146 RP003497695 RP003512811 RP003530176 RP003533410 RP003563374 RP003567550 RP003604521 RP003604746 RP003629750 RP003634303 RP003642400 RP003650166 RP003654717 RP003655850 RP003657366 RP003670485 RP003670876 RP003677047 RP003682132 RP004227086 RP004375393
RNAi & Array Information
Linkouts	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42642
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: AP-50 anon-EST:Posey50 (Bayraktaroglu, 2000.11.2) Source for merge of: AP-50 anon-WO0172774.104 (FlyBase, 1996-) Source for merge of: AP-50 MENE(3R)-D (Windler and Bilder, 2010)
Additional comments	Source for merge of AP-50 anon-WO0172774.104 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142792 NM_170015 NM_001170215 RefSeq (Proteins) • NP_651049 NP_732744 NP_001163686 DDBJ / EMBL / GenBank • AA141728 AA141729 AA567297 AAF14248 AAF56001 AAF56002 AAL48183 ACZ94982 AF083300 AF110232 AI533540 AJ005962 AW944413 AX260142 AY070712 CAA06785 Entrez Gene - A searchable database of RefSeq genes. • 42642 UniProtKB/TrEMBL • O62530
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Clathrin adaptor, mu subunit (IPR001392) Clathrin adaptor, mu subunit, C-terminal (IPR008968) Longin-like (IPR011012) Clathrin adaptor, mu subunit, conserved site (IPR018240) Clathrin coat associated protein AP-50 (IPR015629) AP complex, mu/sigma subunit (IPR022775)
Linkouts
	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42642
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	anon-EST:Posey50   anon-WO0172774.104   AP-2μ (Windler and Bilder, 2010) AP-50 (Benhra et al., 2011, Asensio et al., 2010, Akashi et al., 2006, Stuart et al., 2007, Geng and MacDonald, 2007, Saleh et al., 2006, Yang et al., 2011) AP50 (Bauer-DuMont et al., 2004, Begun, 2001, Begun and Whitley, 2000, Zhang, 1998.5.6, Zhang and Broadie, 1999, Kametaka et al., 2010) cg7057 (Boehm and Bonifacino, 2002) CG7057 (Boehm, 2001, Verstreken and Bellen, 2000.9.4, Saleh et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009, Cammarato et al., 2011) Dμ2 (Jullien-Flores et al., 2000) MENE(3R)-D (Windler and Bilder, 2010) MENE (3R)-D (Menut et al., 2007) μ2 (Boehm and Bonifacino, 2001, Mullins et al., 1999)
Name Synonym	AP-50 (Cammarato et al., 2011)
Secondary FlyBase IDs
	FBgn0024832 FBgn0025529 FBgn0046487 FBgn0260567
References ( 54 )
Generate a list of	All references relating to this gene ( 54 )
List References by type	Research paper  ( 22 ) Supplementary material  ( 7 ) Review  ( 2 ) Personal communication to FlyBase  ( 9 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 9 ) Computer file  ( 1 ) Patent  ( 1 ) Automatic genome annotation  ( 1 ) Letter  ( 1 )
Recent research papers ( 6 )
	Benhra et al., 2011, Curr. Biol. 21(1): 87--95 AP-1 Controls the Trafficking of Notch and Sanpodo toward E-Cadherin Junctions in Sensory Organ Precursors. [FBrf0212697] Yang et al., 2011, Neuron 72(2): 285--299 Nak regulates localization of clathrin sites in higher-order dendrites to promote local dendrite growth. [FBrf0216477] Asensio et al., 2010, J. Cell Biol. 191(6): 1173--1187 RNAi screen identifies a role for adaptor protein AP-3 in sorting to the regulated secretory pathway. [FBrf0212515] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Kametaka et al., 2010, J. Cell Sci. 123(3): 460--471 Functional characterization of protein-sorting machineries at the trans-Golgi network in Drosophila melanogaster. [FBrf0209912] Windler and Bilder, 2010, Curr. Biol. 20(6): 538--543 Endocytic internalization routes required for delta/notch signaling. [FBrf0210409] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...