• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\mld

General Information
Symbol	Dmel\mld	Species	D. melanogaster
Name	molting defective	Annotation symbol	CG34100
Feature type	protein_coding_gene	FlyBase ID	FBgn0263490
Gene Model Status	Current	Stock availability	30 publicly available
Also Known As	DTS-3, CG33343, l(3)3DTS, CG13620, CG9469
Genomic Location
Chromosome (arm)	3R	Recombination map	3-84.5
Cytogenetic map	96A9-96A12	Sequence location	3R:20,411,375..20,449,621 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene molting defective is referred to in FlyBase by the symbol Dmel\mld (CG34100, FBgn0263490). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: zinc ion binding; nucleic acid binding. There is experimental evidence that it is involved in the biological process: determination of adult lifespan; positive regulation of circadian sleep/wake cycle, sleep; long-term memory; ecdysone biosynthetic process. 38 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum; ring gland. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Zinc finger, AD-type; Zinc finger, C2H2; Zinc finger, C2H2-like; Zinc finger, C2H2-type/integrase, DNA-binding. Gene sequence location is 3R:20411375..20449621.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	l(3)DTS3 Heterozygous larvae raised at 29 arrested at the third lar- val instar for up to two weeks; exhibit grossly hypertrophied ring glands due to the growth of the prothoracic gland cells. The nuclei possess polytene chromosomes which are as large as those of salivary gland nuclei; their banding pattern is simi- lar but not identical to that of salivary gland polytene chro- mosomes, but the puffing patterns are quite different. Salivary gland chromosomes also hypertrophy and lack the nor- mal ecdysone-induced puffs. Chromosomes in both tissues respond to adminstration of ecdysone by producing the charac- teristic set of puffs (Holden and Ashburner, 1978, Chromosoma 68: 205-27). At 29 imaginal discs unable to evert in situ; however evert normally if cultured in the presence of ecdys- terone or in wild-type hosts. Implantation of a single wild- type ring gland rescues lethality (Holden, Walker, Maroy, Wat- son, White, and Gausz, 1986, Dev. Genet. 6: 153-62).
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features HFA13681 HFA14622 BKN30031 BKN30615
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96A9-96A12
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	3-84.5 (Holden and Suzuki, 1973)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mld for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mld-RA FBtr0111012  NM_001043289   6957   1918 mld-RC FBtr0304912     6954   1918 mld-RD FBtr0304913     7098   1965 mld-RE FBtr0304914     7029   1943 mld-RF FBtr0304915     6891   1896
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mld-PA   FBpp0110311   212.9   1918   6.05     NP_001036754   AAN14004 mld-PC   FBpp0293451   212.9   1918   6.05       mld-PD   FBpp0293452   218.7   1965   6.20       mld-PE   FBpp0293453   216.3   1943   6.25       mld-PF   FBpp0293454   210.4   1896   6.11
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2 (IPR007087) Zinc finger, AD-type (IPR012934) Zinc finger, C2H2-like (IPR015880) Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA697105   AA697106   AE014297 AAN14004   AI133971   AI532407   AI543544   AI546295   AW944332   AY118644 AAM50013   BF494642   BG633168   BI352485   BI369831   BI567100   BI585103   BI585122   BI586709   BI620575   BI625001   BI625218   BT031318   BT120145   CK134266   CO157110   CO178036   CO179240   CO190816   CO264854   CO273208   CO275052   CO280280   CO285142   CO295748   CO298626   CO302913   CO303347   CO309303   CO313105   CO314282   CO322038   CO323556   CO326128   CO333009   CW848196   CZ466349   DN154380   EC058100   EC058389   EC060442   EC061887   EC062935   EC072746   EC079323   EC084873   EC085816   EC085916   EC087282   EC088016   EC195778   EC201560   EC203137   EC208874   EC210717   EC213945   EC246335   EC246573   EC254265   EC261081   EC266751   EL887345   EV581941   EV587205   EV592664
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IMW5 Q8MSR6 Q0KI16
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. [an error occurred while processing this directive] modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | dominant | heat sensitive mldDTS3-1 lethal | dominant | larval stage | heat sensitive mldDTS3-1 lethal | dominant | pupal stage | heat sensitive mldDTS3-1 lethal | first instar larval stage (with mld47) mld4425 lethal | first instar larval stage (with mld4425) mld47 lethal | larval stage (with Df(3R)crb87-5) mld22 mld47 mld77 mld83 mld92 lethal | pupal stage, with Scer\GAL4pnr-MD237 mldGD5189 viable mldc00300 mldEY12290 viable, with Scer\GAL4pnr-MD237 mldGD240 mldGD8077 Sterility Allele fertile mldc00300 mldEY12290 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 mldGD5189 courtship behavior defective | conditional | dominant mldDTS3-1 developmental rate defective (with Df(3R)crb87-5) mld47 long lived | dominant | female limited | temperature conditional mldDTS3-1 memory defective | dominant mldDTS3-1 sleep defective | dominant | female mldDTS3-1 visible, with Scer\GAL4pnr-MD237 mldGD5189 Phenotype manifest in Allele larval salivary gland & embryo & nuclear chromosome | conditional ts mldDTS3-1 mesothoracic tergum, with Scer\GAL4pnr-MD237 mldGD5189 prothoracic gland | heat sensitive mldDTS3-1 ring gland (with Df(3R)crb87-5) mld47 ring gland | heat sensitive mldDTS3-1 trichogen cell, with Scer\GAL4pnr-MD237 mldGD5189
Classical Alleles ( 30 )
For All Classical Alleles Show
Allele of mld Class Mutagen Stocks Known lesion mldDTS3-1 ethyl methanesulfonate 3 -- mldc00300 piggyBac transposase 2 -- mldc01261 1 -- mldc01346 1 -- mldd03912 1 -- mldDG25210 P-element activity 1 -- mlde01171 1 -- mlde01774 1 -- mlde01953 1 -- mlde02511 1 -- mldEY12290 P-element activity 1 -- mldEY15999 1 -- mldf03508 1 -- mldf04553 1 -- mldMB02957 1 -- mldMB08505 1 -- mldMI01185 1 -- mld22 ethyl methanesulfonate 0 Yes mld30221 P-element activity 0 Yes mld4425 0 -- mld47 ethyl methanesulfonate 0 Yes mld77 ethyl methanesulfonate 0 Yes mld83 ethyl methanesulfonate 0 Yes mld92 ethyl methanesulfonate 0 Yes mldA069 0 -- mldc01322 0 -- mlde01999 0 -- mldEP3020 P-element activity 0 -- mldS133713 P-element activity 0 -- mldunspecified 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mld Class Mutagen Stocks Known lesion mldGD240 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes mldGD5189 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes mldGD8077 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes mldJF02244 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mldKK110031 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mldKK110392 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mldKK110615 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mldScer\UAS.cNa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)crb87-5 (Neubueser et al., 2005) Df(3R)Exel7357 (Ryder, 2004.4.26) In(3R)Ubx7LLatsR (Neubueser et al., 2005)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD240} NA P{GD5189} NA P{GD8077} NA P{KK110031} NA P{KK110392} NA P{KK110615} NA P{TRiP.JF02244} NA P{UAS-mld.N} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}mld30221 NA P{EP}mldEP3020 NA P{EPgy2}mldEY12290 NA P{EPgy2}mldEY15999 NA P{GSV1}GSd456GSd456 NA P{XP}mldd03912 NA miscellaneous insertions Mi{MIC}mldMI01185 NA P{wHy}mldDG25210 NA PBac{5HPw+}mldA069 NA PBac{PB}mldc00300 NA PBac{PB}mldc01261 NA PBac{PB}mldc01322 NA PBac{PB}mldc01346 NA PBac{RB}mlde01171 NA PBac{RB}mlde01774 NA PBac{RB}mlde01953 NA PBac{RB}mlde01999 NA PBac{RB}mlde02511 NA PBac{WH}mldf03508 NA PBac{WH}mldf04553 NA insertion of enhancer trap P{lacW}mldS133713 No insertion of enhancer trap binary system Mi{ET1}mldMB02957 No Mi{ET1}mldMB08505 No
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
determination of adult lifespan inferred from mutant phenotype (Simon et al., 2003) ecdysone biosynthetic process inferred from mutant phenotype (Neubueser et al., 2005) long-term memory inferred from mutant phenotype (Ishimoto et al., 2009) positive regulation of circadian sleep/wake cycle, sleep inferred from mutant phenotype (Ishimoto and Kitamoto, 2010)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Neubueser et al., 2005)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
nucleic acid binding inferred from electronic annotation with InterPro:IPR013087 (FlyBase Curators et al., 2004-) zinc ion binding inferred from electronic annotation with InterPro:IPR007087, InterPro:IPR012934, InterPro:IPR015880 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mld allele Gene References
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dpse\GA12413   Dpse\GA16171
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 30 )
Bloomington	3014 mldDTS3-1/TM2
Harvard	- P{XP}mldd03912 - PBac{PB}mldc00300 - PBac{PB}mldc01261 - PBac{PB}mldc01346 - PBac{RB}mlde01171 - PBac{RB}mlde01774 - PBac{RB}mlde01953
Kyoto	106783 mldDTS3-1/TM2
VDRC	v101867 P{KK110031}VIE-260B v102023 P{KK110392}VIE-260B v108478 P{KK110615}VIE-260B
	More stocks available...
Genomic Clones ( 2 )
	BACR35N03 BACR47D16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 67 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD03914
Other clones	IP14518 MIP16052
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT03345 GH11291 GH21560 GM09091 GM09092 GM19472 LD47835 RH24572 RH24596 RH26794 RH36946 RH51747 RH64789 RH65510 SD10337
Other clones	10199 20021 40524 40588 42482 60175 60627 60962 61405 63951 78244 82088 106077 106304 121223 214479.4 280609 524708 533975 CK01.62A.F9 CK01.68D.G2 CK01.75A.A4 CK01.143B.C3 Dmel_dig_013741_0700_2004 Dmel_neb_011236_0117_0598 Dmel_neb_014575_0672_1229 EK049823 EK055043 EK058834 EK110051 EK116310 EK140507 EK153611 EK168334 EK173361 EK185141 EK187061 EK190820 EK211517 EK217313 EK231360 EK231724 EK235624 EK241135 EK305420 EN09946 EP03263 IP18544 RE56144
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: mld CG33343 CG31312 CG13620 (FlyBase Genome Annotators, 2006) Source for merge of: mld l(3)DTS3 (Ishimoto and Kitamoto, 2010)
Additional comments	Annotations CG33343, CG31312 and CG13620 merged as CG34100 (which corresponds to mld) in release 5.1 of the genome annotation. (FlyBase Genome Annotators, 2006) FlyBase curator comment: The symbol "CG9469" has been used erroneously in FlyBase to name 2 separate annotations (one on chromosome arm 2R, the other on arm 3R) in different releases of the genome annotation. The two annotations have been renamed in release 3.2 to avoid confusion; they are CG33352 (on 2R) and CG33343 (on 3R). CG33352 was labelled "CG9469" in releases 1 and 2 and was not annotated in releases 3.0 and 3.1 of the genome annotation. CG33343 was a new annotation in release 3.0 of the genome annotation and was labelled "CG9469" in releases 3.0 and 3.1. (FlyBase Genome Annotators, 2004)
Other Comments
	mld corresponds to the first two exons of CG33343 and exons two to five of CG13620. (Neubueser et al., 2005) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) New annotation (CG31312) in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_001043289 RefSeq (Proteins) • NP_001036754 DDBJ / EMBL / GenBank • AAM50013 AAN14004 ABI31205 AI532407 AW944332 AY118644 BI369831 CW848196 CZ466349 Entrez Gene - A searchable database of RefSeq genes. • 2768685 UniProtKB/TrEMBL • Q0KI16 Q8IMW5 Q8MSR6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, C2H2 (IPR007087) Zinc finger, AD-type (IPR012934) Zinc finger, C2H2-like (IPR015880) Zinc finger, C2H2-type/integrase, DNA-binding (IPR013087)
Linkouts
Synonyms & Secondary IDs ( 17 )
Reported As
Symbol Synonym	CG9469 (Ringrose et al., 2003, Boutros et al., 2004, ) CG13620 (Neubueser et al., 2005, Giot et al., 2003, ) CG31312   CG33343 (Neubueser et al., 2005, Myrick et al., 2005.5.16, Quinones-Coello, 2007) CG34100 (FlyBase Genome Annotators, 2006, Deloger et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009) DTS-3 (Simon et al., 2003, Komonyi et al., 2001, Awad and Truman, 1997, Truman et al., 1993, Segal, 1993, Holden and Suzuki, 1973, Ishimoto et al., 2009) l(3)3DTS (Kozlova and Thummel, 2000, Riddiford, 1993) L(3)3DTS (Walker et al., 1987) l(3)DTS3   mld (Neubueser et al., 2005, Ono et al., 2006, FlyBase Curators, 2008, Minidorff et al., 2007, Ishimoto and Kitamoto, 2010, Xiang et al., 2010) Mld (Deloger et al., 2009, Talamillo et al., 2008)
Name Synonym	defective (Ishimoto and Kitamoto, 2010) Dominant temperature-sensitive 3 (Ishimoto et al., 2009) lethal (3) DTS3   molting (Ishimoto and Kitamoto, 2010) molting defective (Neubueser et al., 2005, ) Molting defective (Talamillo et al., 2007, Talamillo et al., 2008)
Secondary FlyBase IDs
	FBan0013620 FBgn0002416 FBgn0033118 FBgn0039211 FBgn0051312 FBgn0053343 FBgn0083077
References ( 50 )
Generate a list of	All references relating to this gene ( 50 )
List References by type	Research paper  ( 15 ) Supplementary material  ( 5 ) Review  ( 4 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 3 ) FlyBase analysis  ( 6 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 2 )
	Ishimoto and Kitamoto, 2010, Genetics 185(1): 269--281 The Steroid Molting Hormone Ecdysone Regulates Sleep in Adult Drosophila melanogaster. [FBrf0210911] Xiang et al., 2010, Dev. Biol. 344(2): 800--808 br regulates the expression of the ecdysone biosynthesis gene npc1. [FBrf0211381] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...