FlyBase Gene Report: Dmel\nclb

General Information

Symbol

Dmel\nclb

Species

D. melanogaster

Name

no child left behind

Annotation Symbol

CG6751

Feature Type

protein_coding_gene

FlyBase ID

FBgn0263510

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

Chromatin-associated factor that regulates transcription (PubMed:21752937). Regulates Pol I-mediated rRNA biogenesis and, probably, Pol III-mediated transcription (PubMed:29065309). Regulates the localization to the nucleolus of Cdk7, a regulator of the Pol I-elongation factor TFIIH (PubMed:29065309). Acts as a regulator of cell proliferation and tissue growth as part of the TORC1 and Myc signaling pathway in response to nutrients (PubMed:29065309). Required in males for both germline stem cell (GSC) maintenance and early stages of germ cell differentiation of germ cell cysts (PubMed:21752937). Not required for female germline stem cell (GSC) maintenance, but necessary to regulate germ cell differentiation and egg chamber development (PubMed:21752937). In female somatic cells, required for follicle stem cell survival and maintenance (PubMed:21752937). (UniProt, A1Z8D0)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

dPWP1, PWP1

Key Links

Genomic Location

Cytogenetic map

47C5-47C5

Sequence location

2R:10,875,748..10,877,296 [+]

Recombination map

2-63

RefSeq locus

NT_033778 REGION:10875748..10877296

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables chromatin DNA binding

inferred from mutant phenotype

(Casper et al., 2011)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (12 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

involved_in cellular response to insulin stimulus

inferred from direct assay

(Liu et al., 2017)

involved_in follicle cell of egg chamber development

inferred from mutant phenotype

(Casper et al., 2011)

involved_in germ cell development

inferred from mutant phenotype

(Casper et al., 2011)

involved_in male germ-line stem cell population maintenance

inferred from mutant phenotype

(Casper et al., 2011)

involved_in male gonad development

inferred from mutant phenotype

(Casper et al., 2011)

involved_in ovarian fusome organization

inferred from mutant phenotype

(Casper et al., 2011)

involved_in positive regulation of ribosome biogenesis

inferred from direct assay

(Liu et al., 2017)

involved_in positive regulation of transcription by RNA polymerase III

inferred from direct assay

(Liu et al., 2017)

involved_in positive regulation of transcription of nucleolar large rRNA by RNA polymerase I

inferred from direct assay

(Liu et al., 2017)

involved_in regulation of gene expression

inferred from mutant phenotype

(Casper et al., 2011)

NOT involved_in female germ-line stem cell population maintenance

inferred from mutant phenotype

(Casper et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in rRNA processing

inferred from electronic annotation with InterPro:IPR044285

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in nucleolus

inferred from direct assay

(Liu et al., 2017)

located_in nucleoplasm

inferred from direct assay

(Liu et al., 2017)

located_in nucleus

inferred from direct assay

(Casper et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000363374

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the WD repeat PWP1 family. (A1Z8D0)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

(UniProt, A1Z8D0)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\nclb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z8D0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

nclb-RA

FBtr0088228

NM_136779

1549

459

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

nclb-PA

FBpp0087323

51.4

459

4.00

A1Z8D0

NP_610623

AAF58738

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with Mybbp1A.

(UniProt, A1Z8D0)

Post Translational Modification

Phosphorylated in response to nutrient-activated TORC1 signaling.

(UniProt, A1Z8D0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nclb using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

nclb_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.02

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

foregut anlage

(Fisher et al., 2012)

hindgut anlage

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

anterior endoderm

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

fat body primordium

Comment: reported as fat body specific anlage

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

longitudinal trunk visceral muscle primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

somatic muscle primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

trunk visceral muscle primordium

(Fisher et al., 2012)

embryonic stage 17

germline cell

(Casper and Van Doren, 2009)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

germline cell

(Casper et al., 2011)

adult stage | female

somatic cell of ovariole

(Casper et al., 2011)

female germline cell

(Casper et al., 2011)

adult stage | male

testis

(Casper et al., 2011)

male germline cell

(Casper et al., 2011)

Additional Descriptive Data

nclb protein is detected in a broad range of embryonic cell types including the germ cells. It is detected in germline cells in the testis and ovary and also in somatic cells in the ovary.

(Casper et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleolus

inferred from direct assay

(Liu et al., 2017)

located_in nucleoplasm

inferred from direct assay

(Liu et al., 2017)

located_in nucleus

inferred from direct assay

(Casper et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\nclb in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\nclb

View exonic expression by tissue for Dmel\nclb

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263510

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36150/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263510

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033562

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263510

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6751

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of nclb

Transgenic constructs containing regulatory region of nclb

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)Exel6059

(Ryder, 2004.4.26)

Df(2R)nclb

(Casper et al., 2011)

Inferred from location ( 10 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage

nclb²

lethal - all die during larval stage | recessive

nclb²

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

nclb^GD4073

partially lethal - majority die, with Scer\GAL4^pnr-MD237

nclb^GD4073

partially lethal - majority die (with nclb¹)

nclb²

partially lethal - majority die (with nclb²)

nclb¹

viable

viable, with Scer\GAL4^tin.CΔ4

nclb^GD4073

viable | adult stage

nclb^EY10712

Sterility

Allele

female semi-fertile

nclb^EY10712

fertile

nclb^EY10712

male sterile

nclb^EY10712

sterile

nclb¹

Other Phenotypes

Allele

abnormal developmental rate | larval stage

nclb²

abnormal developmental rate | larval stage (with nclb¹)

nclb²

abnormal developmental rate | larval stage (with nclb²)

nclb¹

abnormal developmental rate | pupal stage, with Scer\GAL4^Cg.PA

nclb^NIG.6751R

abnormal endomitotic cell cycle

abnormal endomitotic cell cycle (with nclb¹)

nclb²

abnormal endomitotic cell cycle (with nclb²)

nclb¹

abnormal size | adult stage, with Scer\GAL4^en-e16E

nclb^NIG.6751R

abnormal size | adult stage (with nclb¹)

nclb²

abnormal size | adult stage (with nclb²)

nclb¹

decreased body size | larval stage

nclb²

decreased body size | larval stage (with nclb¹)

nclb²

decreased body size | larval stage (with nclb²)

nclb¹

decreased body size | larval stage | recessive

decreased body size | pupal stage, with Scer\GAL4^Cg.PA

nclb^NIG.6751R

decreased body size | pupal stage (with nclb¹)

nclb²

decreased body size | pupal stage (with nclb²)

nclb¹

some die during pupal stage, with Scer\GAL4^pnr-MD237

nclb^GD4073

some die during third instar larval stage

nclb²

visible, with Scer\GAL4^pnr-MD237

nclb^GD4073

Phenotype manifest in

Allele

egg chamber | oogenesis stage S5

egg chamber | oogenesis stage S5 (with nclb¹)

nclb²

egg chamber | oogenesis stage S5 (with nclb²)

nclb¹

egg chamber | oogenesis stage S5 | somatic clone

nclb²

female germline cell

female germline cell (with nclb¹)

nclb²

female germline cell (with nclb²)

nclb¹

female germline cell | somatic clone

nclb²

follicle cell | somatic clone

nclb²

individualization stage spermatid

individualization stage spermatid (with nclb¹)

nclb²

individualization stage spermatid (with nclb²)

nclb¹

macrochaeta | adult stage (with nclb¹)

nclb²

macrochaeta | adult stage (with nclb²)

nclb¹

male germline cell

male germline cell (with nclb¹)

nclb²

male germline cell (with nclb²)

nclb¹

mesonotum, with Scer\GAL4^pnr-MD237

nclb^GD4073

nurse cell | oogenesis stage S5

nurse cell | oogenesis stage S5 (with nclb¹)

nclb²

nurse cell | oogenesis stage S5 (with nclb²)

nclb¹

nurse cell | oogenesis stage S5 | somatic clone

nclb²

ovary

ovary (with nclb¹)

nclb²

ovary (with nclb²)

nclb¹

testis

testis (with nclb¹)

nclb²

testis (with nclb²)

nclb¹

trichogen cell, with Scer\GAL4^pnr-MD237

nclb^GD4073

wing posterior compartment, with Scer\GAL4^en-e16E

nclb^NIG.6751R

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (45)

Hsap\PWP1

14 of 14

Yes

Hsap\CHAF1B

1 of 14

Hsap\CIAO1

1 of 14

Hsap\COP1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\WDR77

1 of 14

Hsap\WDR83

1 of 14

Hsap\WDR89

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (31)

Rnor\Pwp1

14 of 14

Yes

Rnor\Cop1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Wdr77

1 of 14

Rnor\Wdr89

1 of 14

Mus musculus (laboratory mouse) (29)

Mmus\Pwp1

13 of 14

Yes

Mmus\Cop1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Wdr77

1 of 14

Mmus\Wdr89

1 of 14

Xenopus tropicalis (Western clawed frog) (26)

Xtro\pwp1

11 of 13

Yes

Xtro\slc39a2

9 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (31)

Drer\pwp1

14 of 14

Yes

Drer\cop1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\wdr89

1 of 14

Drer\wsb1

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (15)

Cele\JC8.2

12 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (23)

Agam\AgaP_AGAP004768

11 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (53)

Atha\AT4G18900

13 of 13

Yes

Atha\AT4G18905

13 of 13

Yes

Atha\AT4G35370

13 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\DRS1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\SPA3

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\TTG1

1 of 13

Atha\VIP3

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (14)

Scer\PWP1

13 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (10)

Spom\SPBC1711.16

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:nclb. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (25)

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

ebi

1 of 13

fzr

1 of 13

kat80

1 of 13

l(2)09851

1 of 13

Nle

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

vls

1 of 13

wap

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PWP1; PWP1 homolog, endonuclein

14 of 14

620055

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

nclb - Mybbp1A

anti tag coimmunoprecipitation, anti tag western blot

(Liu et al., 2017)

nclb - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Erk7

suppressible

nclb

(Hasygar et al., 2021)

suppressible

(Liu et al., 2017)

suppressible

(Liu et al., 2017)

suppressible

(Liu et al., 2017)

External Data

Subunit Structure (UniProtKB)

Interacts with Mybbp1A.

(UniProt, A1Z8D0 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0263510

MIST (protein-protein) - An integrated Molecular Interaction Database

36150

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A1Z8D0

Class of Gene

gene

(FlyBase, 1992-)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-63

Cytogenetic map

47C5-47C5

Sequence location

2R:10,875,748..10,877,296 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

47C5-47C5

Limits computationally determined from genome sequence between P{lacW}l(2)k15826^k15826&P{EP}EP471 and P{EP}shn^EP2359&P{EP}shn^EP644

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-63

(FlyBase, 2016)

2-62.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

41826

y¹ v¹; P{TRiP.GL01254}attP2

20206

y¹ w^67c23; P{EPgy2}nclb^EY10712

21138

y¹ w^67c23; P{EPgy2}nclb^EY15483/CyO

83607

y¹ sc^* v¹ sev²¹; P{TOE.GS05268}attP40

6751R-1

6751R-3

v107563

v12577

w¹¹¹⁸; P{GD4073}v12577

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (38)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE21021

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0263510

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Germ cells can enter differentiation but spermatogenic cysts cannot develop normally in nclb mutant testes.

(Casper et al., 2011)

nclb is not required for the viability of male germline stem cells, but is required for proper germline stem cell maintenance and for germ cell cysts to complete spermatogenesis once they have initiated differentiation.

(Casper et al., 2011)

nclb is required in the female germline for germ cell differentiation and egg chamber development, but not for germline stem cell maintenance.

(Casper et al., 2011)

nclb is not required in differentiated follicle cells, but is required in follicle stem cells for their survival or maintenance.

(Casper et al., 2011)

The total amount of active transcription is reduced in nclb mutants.

(Casper et al., 2011)

Relationship to Other Genes

Source for database merge of

Source for merge of: nclb CG6751

(Casper et al., 2011)

Additional comments

"CG6751" may correspond to "Scim24". Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places "Scim24" near/in "CG6751" or "Taf80".

(Dobie and Karpen, 2001.4.18)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Owing to the defects in fertility and germline development associated with this gene, the authors have named it 'no child left behind'.

(Casper et al., 2011)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Name Synonyms

No child left behind

(Mehrotra and Deshpande, 2018)

no child left behind

(Dorn and Dorn, 2015, Lim et al., 2012, Casper et al., 2011, Casper and Doren, 2008)

Secondary FlyBase IDs

FBgn0259115
FBgn0033562

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 31 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36150

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z8D0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A1Z8D0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z8D0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263510

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36150/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263510

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263510

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6751

MARRVEL_MODEL - MARRVEL (model organism gene)

36150

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0263510

DroID - A comprehensive database of gene and protein interactions.

FBgn0263510

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

nclb_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0033562

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0263510

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6751

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007893

MIST (protein-protein) - An integrated Molecular Interaction Database

36150

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A1Z8D0

References (61)

The following external sites may use different assemblies or annotations than FlyBase.

0.02

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