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Gene Dmel\Vha68-2

General Information
Symbol	Dmel\Vha68-2	Species	D. melanogaster
Name	Vacuolar H+ ATPase 68 kDa subunit 2	Annotation symbol	CG3762
Feature type	protein_coding_gene	FlyBase ID	FBgn0263598
Gene Model Status	Current	Stock availability	13 publicly available
Also Known As	Vha68
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	34A3-34A3	Sequence location	2L:12,974,322..12,978,607 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Vacuolar H+ ATPase 68 kDa subunit 2 is referred to in FlyBase by the symbol Dmel\Vha68-2 (CG3762, FBgn0263598). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: ATP binding; proton-transporting ATPase activity, rotational mechanism. There is experimental evidence that it is involved in the biological process: endosomal lumen acidification; imaginal disc growth. 22 alleles are reported. The phenotypes of these alleles are annotated with: Malpighian tubule; mesothoracic tergum; embryonic/larval Malpighian tubule. It has 6 annotated transcripts and 6 annotated polypeptides. Protein features are: ATPase, F1 complex beta subunit/V1 complex, C-terminal; ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal; ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain; ATPase, V1 complex, subunit A; ATPase, alpha/beta subunit, N-terminal; ATPase, alpha/beta subunit, nucleotide-binding domain, active site; V-type ATP synthase catalytic subunit A/alpha. Gene sequence location is 2L:12974322..12978607.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03	Controlled Vocabulary Terms endosomal transport
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 34A3-34A3   Limits computationally determined from genome sequence between P{lacW}l(2)k07015k07015&P{PZ}l(2)rK639rK639 and P{lacW}l(2)k11328k11328&P{lacW}l(2)k10105k10105
Experimentally Determined Cytological Location
Cytogenetic map Notes References 34A1-34A2   (determined by in situ hybridisation)   (Spradling et al., 1999) 34B-34B     (Dow, 1999) 34B-34B     (Dow et al., 1997) 34B1-34B12     (Dow et al., 1997) 34A1-34A2   34A3--4   (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Vha68-2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Vha68-2-RA FBtr0080418  NM_143747   2486   614 Vha68-2-RB FBtr0080419  NM_165021   2464   614 Vha68-2-RC FBtr0080420  NM_165022   2531   614 Vha68-2-RD FBtr0305551  NM_001259086   2444   614 Vha68-2-RE FBtr0336613  NM_001273495   2474   614 Vha68-2-RF FBtr0336614  NM_001273496   2528   614
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Vha68-2-PA   FBpp0079999   68.3   614   4.98     NP_652004   AAF53231 Vha68-2-PB   FBpp0080000   68.3   614   4.98     NP_723775   AAF53233 Vha68-2-PC   FBpp0080001   68.3   614   4.98     NP_723776   AAF53232 Vha68-2-PD   FBpp0294002   68.3   614   4.98     NP_001246015   AFH03689 Vha68-2-PE   FBpp0307596   68.3   614   4.98     NP_001260424   AGB92959 Vha68-2-PF   FBpp0307597   68.3   614   4.98     NP_001260425   AGB92960
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • ATPase, F1 complex beta subunit/V1 complex, C-terminal (IPR024034) ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain (IPR000194) ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal (IPR000793) ATPase, alpha/beta subunit, N-terminal (IPR004100) ATPase, V1 complex, subunit A (IPR005725) ATPase, alpha/beta subunit, nucleotide-binding domain, active site (IPR020003) V-type ATP synthase catalytic subunit A/alpha (IPR022878) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263818   AA264223   AA264580   AA438800   AA439497   AA440138   AA440470   AA441065   AA540681   AA695393   AA697115   AA802664   AA802916   AA978968   AE014134 AAF53231   AE014134 AAF53232   AE014134 AAF53233   AE014134 AFH03689   AE014134 AGB92959   AE014134 AGB92960   AI061824   AI063081   AI064331   AI106580   AI109056   AI109831   AI113682   AI114011   AI133830   AI134941   AI260924   AI512615   AI515826   AI530772   AI533512   AI541784   AQ073265   AQ254852   AY084150 AAL89888   AY089512   BF495161   BF499396   BF500086   BG640195   BG640330   BI162336   BI162337   BI163450   BI172925   BI212246   BI228543   BI229858   BI234795   BI235842   BI236139   BI239339   BI362896   BI369805   BI481707   BI481848   BI486058   BI486153   BI564111   BI566584   BI566706   BI566901   BI567190   BI570940   BI572443   BI574936   BI576611   BI577977   BI580430   BI580555   BI582240   BI584172   BI585785   BI589074   BI589076   BI591490   BI605896   BI610171   BI610870   BI615313   BI618382   BI626154   BP547753   BP556172   BP556173   BQ103250   BT124897   CA806353   CA806916   CA807774   CK132176   CK658586   CK660493   CK661693   CO189320   CO195739   CO269961   CO270361   CO285480   CO294979   CO300026   CO302886   CO303759   CO306994   CO309688   CO311397   CO311665   CO318391   CO330494   CO332020   CO332154   CO333719   CO338569   CZ475403   CZ489581   EC062455   EC064540   EC064921   EC066789   EC067771   EC074077   EC076284   EC076650   EC079232   EC208656   EC215591   EC216307   EC217700   EC217724   EC223824   EC225344   EC234235   EC243196   EC249351   EC263510   ES695672   ES695682   ES697008   EV578134   EV579246   EV579536   EV582003   EV586674   EV588790   EV590308   EV592003   EV592861   EV594879   EV595521   EV598205   GH748070   GH749183   GH751996   GH752330   GH752672   GH752987   GH753097   GH755691   GH758585   GH771423   GH772581   GH772977   GH776553   GH776665   GH776674   GH777139   GH778342   GH780115   GH783636   GH784323   GH788717   GH797119   GH799540   GH804473   GH807385   GH809207   GH809844   GH810690   GH811098   GH813831   GH814153   GH817903   GH818503   GH823516   GH825293   GH825388   GH825858   GH827795   GH830371   GH835131   GH835397   GH836931   GH840425   GH847488   GH856348   GH857081   GH857446   GH857704   GH858213   GH858457   GH858924   GH859700   GH859730   GH860924   GH861348   GH862607   GH863206   GH867054   GH868693   GH872303   GH874519   GH876045   GH876262   GH878991   GH880761   GH883959   GH884052   GH884156   GH886892   GH887527   GH888798   GH889648   GH892146   GH894160   GH894298   GH896411   GH900645   GH900701   GH902042   GH902213   GH902779   GH904465   GH904762   GH905296   GH909203   GH911002   GH911246   GH912167   GH912300   GH913536   GH914000   GH915635   GH916421   GH916829   GH920096   GH920644   GH921658   GH923408   GH925407   GH927988   GH928800   GH929606   GH930572   GH932942   GH938904   U59146 AAB02270   U59147 AAB02271
UniProtKB/Swiss-Prot	Q27331
UniProtKB/TrEMBL	A4V0N4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) Comment:rapidly degraded organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) embryonic stage 9 -- 10   head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   amnioserosa   (Fisher et al., 2012) anterior midgut primordium   (Fisher et al., 2012) dorsal pharyngeal muscle primordium   (Fisher et al., 2012) garland cell primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) plasmatocyte primordium   (Fisher et al., 2012) Comment:reported as plasmatocytes anlage posterior midgut primordium   (Fisher et al., 2012) somatic muscle primordium   (Fisher et al., 2012) yolk nucleus   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic anal pad   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic Malpighian tubule   (Fisher et al., 2012) embryonic/larval garland cell   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) gastric caecum   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) plasmatocyte   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Dorus et al., 2006) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
plasma membrane inferred from direct assay (Khanna et al., 2010) plasma membrane proton-transporting V-type ATPase complex inferred from mutant phenotype (Allan et al., 2005)
Expression Deduced from Reporters
Reporter: P{lacW}Vha68-2k02508 Stage Tissue/Position (including subcellular localization) Reference adult stage   Malpighian tubule Type I cell   (Sozen et al., 1997) main segment of Malpighian tubule   (Sozen et al., 1997) Reporter: P{PZ}Vha68-201510 Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval peripheral nervous system   (Jackle and Janning, 1998.8.10) presumptive embryonic/larval central nervous system   (Jackle and Janning, 1998.8.10) embryonic/larval hindgut   (Jackle and Janning, 1998.8.10) anterior embryonic/larval midgut   (Jackle and Janning, 1998.8.10) posterior embryonic/larval midgut   (Jackle and Janning, 1998.8.10) wandering third instar larval stage   embryonic/larval foregut   (Meister and Braun, 1995.10) embryonic/larval midgut   (Meister and Braun, 1995.10) Malpighian tubule   (Meister and Braun, 1995.10) embryonic/larval hindgut   (Meister and Braun, 1995.10) adult stage   optic lobe   (Vosshall, 1998.8.10)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    164 central nervous system, larvae L3    67 central nervous system, pupae P8    30 head, virgin 1-day female    68 head, virgin 4-day female    56 head, virgin 20-day female    51 head, mated 1-day female    65 head, mated 4-day female    44 head, mated 20-day female    46 head, mated 1-day male    88 head, mated 4-day male    66 head, mated 20-day male    93 salivary gland, larvae L3 wandering    82 salivary gland, white prepupae    121 digestive system, larvae L3 wandering    271 digestive system, 1-day adult    795 digestive system, 4-day adult    751 digestive system, 20-day adult    724 fat body, larvae L3 wandering    58 fat body, white prepupae    75 fat body, pupae P8    90 carcass, larvae L3 wandering    195 carcass, 1-day adult    108 carcass, 4-day adult    118 carcass, 20-day adult    122 ovary, virgin 4-day female    273 ovary, mated 4-day female    278 testis, mated 4-day male    53 accessory gland, mated 4-day male    92 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (164) central nervous system, larvae L3  (67) central nervous system, pupae P8  (30) head, virgin 1-day female  (68) head, virgin 4-day female  (56) head, virgin 20-day female  (51) head, mated 1-day female  (65) head, mated 4-day female  (44) head, mated 20-day female  (46) head, mated 1-day male  (88) head, mated 4-day male  (66) head, mated 20-day male  (93) salivary gland, larvae L3 wandering  (82) salivary gland, white prepupae  (121) digestive system, larvae L3 wandering  (271) digestive system, 1-day adult  (795) digestive system, 4-day adult  (751) digestive system, 20-day adult  (724) fat body, larvae L3 wandering  (58) fat body, white prepupae  (75) fat body, pupae P8  (90) carcass, larvae L3 wandering  (195) carcass, 1-day adult  (108) carcass, 4-day adult  (118) carcass, 20-day adult  (122) ovary, virgin 4-day female  (273) ovary, mated 4-day female  (278) testis, mated 4-day male  (53) accessory gland, mated 4-day male  (92) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (164) central nervous system, larvae L3    67 central nervous system, pupae P8    30 head, virgin 1-day female    68 head, virgin 4-day female    56 head, virgin 20-day female    51 head, mated 1-day female    65 head, mated 4-day female    44 head, mated 20-day female    46 head, mated 1-day male    88 head, mated 4-day male    66 head, mated 20-day male    93 salivary gland, larvae L3 wandering    82 salivary gland, white prepupae  (121) digestive system, larvae L3 wandering  (271) digestive system, 1-day adult  (795) digestive system, 4-day adult  (751) digestive system, 20-day adult  (724) fat body, larvae L3 wandering    58 fat body, white prepupae    75 fat body, pupae P8    90 carcass, larvae L3 wandering  (195) carcass, 1-day adult  (108) carcass, 4-day adult  (118) carcass, 20-day adult  (122) ovary, virgin 4-day female  (273) ovary, mated 4-day female  (278) testis, mated 4-day male    53 accessory gland, mated 4-day male    92 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    164 central nervous system, larvae L3    67 central nervous system, pupae P8    30 head, virgin 1-day female    68 head, virgin 4-day female    56 head, virgin 20-day female    51 head, mated 1-day female    65 head, mated 4-day female    44 head, mated 20-day female    46 head, mated 1-day male    88 head, mated 4-day male    66 head, mated 20-day male    93 salivary gland, larvae L3 wandering    82 salivary gland, white prepupae    121 digestive system, larvae L3 wandering    271 digestive system, 1-day adult    795 digestive system, 4-day adult    751 digestive system, 20-day adult    724 fat body, larvae L3 wandering    58 fat body, white prepupae    75 fat body, pupae P8    90 carcass, larvae L3 wandering    195 carcass, 1-day adult    108 carcass, 4-day adult    118 carcass, 20-day adult    122 ovary, virgin 4-day female    273 ovary, mated 4-day female    278 testis, mated 4-day male    53 accessory gland, mated 4-day male    92 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    164 central nervous system, larvae L3    67 central nervous system, pupae P8    30 head, virgin 1-day female    68 head, virgin 4-day female    56 head, virgin 20-day female    51 head, mated 1-day female    65 head, mated 4-day female    44 head, mated 20-day female    46 head, mated 1-day male    88 head, mated 4-day male    66 head, mated 20-day male    93 salivary gland, larvae L3 wandering    82 salivary gland, white prepupae    121 digestive system, larvae L3 wandering    271 digestive system, 1-day adult    795 digestive system, 4-day adult    751 digestive system, 20-day adult    724 fat body, larvae L3 wandering    58 fat body, white prepupae    75 fat body, pupae P8    90 carcass, larvae L3 wandering    195 carcass, 1-day adult    108 carcass, 4-day adult    118 carcass, 20-day adult    122 ovary, virgin 4-day female    273 ovary, mated 4-day female    278 testis, mated 4-day male    53 accessory gland, mated 4-day male    92 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (164) central nervous system, larvae L3  (67) central nervous system, pupae P8  30 head, virgin 1-day female  (68) head, virgin 4-day female  (56) head, virgin 20-day female  (51) head, mated 1-day female  (65) head, mated 4-day female  (44) head, mated 20-day female  (46) head, mated 1-day male  (88) head, mated 4-day male  (66) head, mated 20-day male  (93) salivary gland, larvae L3 wandering  (82) salivary gland, white prepupae  (121) digestive system, larvae L3 wandering  (271) digestive system, 1-day adult  (795) digestive system, 4-day adult  (751) digestive system, 20-day adult  (724) fat body, larvae L3 wandering  (58) fat body, white prepupae  (75) fat body, pupae P8  (90) carcass, larvae L3 wandering  (195) carcass, 1-day adult  (108) carcass, 4-day adult  (118) carcass, 20-day adult  (122) ovary, virgin 4-day female  (273) ovary, mated 4-day female  (278) testis, mated 4-day male  (53) accessory gland, mated 4-day male  (92) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (164) central nervous system, larvae L3    67 central nervous system, pupae P8    30 head, virgin 1-day female    68 head, virgin 4-day female    56 head, virgin 20-day female    51 head, mated 1-day female    65 head, mated 4-day female    44 head, mated 20-day female    46 head, mated 1-day male    88 head, mated 4-day male    66 head, mated 20-day male    93 salivary gland, larvae L3 wandering    82 salivary gland, white prepupae  121 digestive system, larvae L3 wandering  (271) digestive system, 1-day adult  (795) digestive system, 4-day adult  (751) digestive system, 20-day adult  (724) fat body, larvae L3 wandering    58 fat body, white prepupae    75 fat body, pupae P8    90 carcass, larvae L3 wandering  (195) carcass, 1-day adult  108 carcass, 4-day adult  118 carcass, 20-day adult  122 ovary, virgin 4-day female  (273) ovary, mated 4-day female  (278) testis, mated 4-day male    53 accessory gland, mated 4-day male    92 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    164 central nervous system, larvae L3    67 central nervous system, pupae P8    30 head, virgin 1-day female    68 head, virgin 4-day female    56 head, virgin 20-day female    51 head, mated 1-day female    65 head, mated 4-day female    44 head, mated 20-day female    46 head, mated 1-day male    88 head, mated 4-day male    66 head, mated 20-day male    93 salivary gland, larvae L3 wandering    82 salivary gland, white prepupae    121 digestive system, larvae L3 wandering    271 digestive system, 1-day adult    795 digestive system, 4-day adult    751 digestive system, 20-day adult    724 fat body, larvae L3 wandering    58 fat body, white prepupae    75 fat body, pupae P8    90 carcass, larvae L3 wandering    195 carcass, 1-day adult    108 carcass, 4-day adult    118 carcass, 20-day adult    122 ovary, virgin 4-day female    273 ovary, mated 4-day female    278 testis, mated 4-day male    53 accessory gland, mated 4-day male    92 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    252 embryo 02-04hr    51 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    41 embryo 10-12hr    87 embryo 12-14hr    102 embryo 14-16hr    254 embryo 16-18hr    348 embryo 18-20hr    470 embryo 20-22hr    751 embryo 22-24hr    742 larva L1    1021 larva L2    722 larva L3 12hr old    569 larva L3 puffstage 1-2    286 larva L3 puffstage 3-6    203 larva L3 puffstage 7-9    147 white prepupae new    100 white prepupae 12hr    123 white prepupae 24hr    58 pupae 2d postWPP    75 pupae 3d postWPP    85 pupae 4d postWPP    261 adult male 01day    259 adult male 05day    221 adult male 30day    248 adult female 01day    427 adult female 05day    295 adult female 30day    294 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (252) embryo 02-04hr  (51) embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr  (41) embryo 10-12hr  (87) embryo 12-14hr  (102) embryo 14-16hr  (254) embryo 16-18hr  (348) embryo 18-20hr  (470) embryo 20-22hr  (751) embryo 22-24hr  (742) larva L1  (1021) larva L2  (722) larva L3 12hr old  (569) larva L3 puffstage 1-2  (286) larva L3 puffstage 3-6  (203) larva L3 puffstage 7-9  (147) white prepupae new  (100) white prepupae 12hr  (123) white prepupae 24hr  (58) pupae 2d postWPP  (75) pupae 3d postWPP  (85) pupae 4d postWPP  (261) adult male 01day  (259) adult male 05day  (221) adult male 30day  (248) adult female 01day  (427) adult female 05day  (295) adult female 30day  (294) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (252) embryo 02-04hr    51 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    41 embryo 10-12hr    87 embryo 12-14hr  102 embryo 14-16hr  (254) embryo 16-18hr  (348) embryo 18-20hr  (470) embryo 20-22hr  (751) embryo 22-24hr  (742) larva L1  (1021) larva L2  (722) larva L3 12hr old  (569) larva L3 puffstage 1-2  (286) larva L3 puffstage 3-6  (203) larva L3 puffstage 7-9  (147) white prepupae new    100 white prepupae 12hr  (123) white prepupae 24hr    58 pupae 2d postWPP    75 pupae 3d postWPP    85 pupae 4d postWPP  (261) adult male 01day  (259) adult male 05day  (221) adult male 30day  (248) adult female 01day  (427) adult female 05day  (295) adult female 30day  (294) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    252 embryo 02-04hr    51 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    41 embryo 10-12hr    87 embryo 12-14hr    102 embryo 14-16hr    254 embryo 16-18hr    348 embryo 18-20hr    470 embryo 20-22hr    751 embryo 22-24hr    742 larva L1    1021 larva L2    722 larva L3 12hr old    569 larva L3 puffstage 1-2    286 larva L3 puffstage 3-6    203 larva L3 puffstage 7-9    147 white prepupae new    100 white prepupae 12hr    123 white prepupae 24hr    58 pupae 2d postWPP    75 pupae 3d postWPP    85 pupae 4d postWPP    261 adult male 01day    259 adult male 05day    221 adult male 30day    248 adult female 01day    427 adult female 05day    295 adult female 30day    294 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    252 embryo 02-04hr    51 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    41 embryo 10-12hr    87 embryo 12-14hr    102 embryo 14-16hr    254 embryo 16-18hr    348 embryo 18-20hr    470 embryo 20-22hr    751 embryo 22-24hr    742 larva L1    1021 larva L2    722 larva L3 12hr old    569 larva L3 puffstage 1-2    286 larva L3 puffstage 3-6    203 larva L3 puffstage 7-9    147 white prepupae new    100 white prepupae 12hr    123 white prepupae 24hr    58 pupae 2d postWPP    75 pupae 3d postWPP    85 pupae 4d postWPP    261 adult male 01day    259 adult male 05day    221 adult male 30day    248 adult female 01day    427 adult female 05day    295 adult female 30day    294 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (252) embryo 02-04hr  (51) embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr  (41) embryo 10-12hr  (87) embryo 12-14hr  (102) embryo 14-16hr  (254) embryo 16-18hr  (348) embryo 18-20hr  (470) embryo 20-22hr  (751) embryo 22-24hr  (742) larva L1  (1021) larva L2  (722) larva L3 12hr old  (569) larva L3 puffstage 1-2  (286) larva L3 puffstage 3-6  (203) larva L3 puffstage 7-9  (147) white prepupae new  (100) white prepupae 12hr  (123) white prepupae 24hr  (58) pupae 2d postWPP  (75) pupae 3d postWPP  (85) pupae 4d postWPP  (261) adult male 01day  (259) adult male 05day  (221) adult male 30day  (248) adult female 01day  (427) adult female 05day  (295) adult female 30day  (294) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (252) embryo 02-04hr    51 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    41 embryo 10-12hr    87 embryo 12-14hr  102 embryo 14-16hr  (254) embryo 16-18hr  (348) embryo 18-20hr  (470) embryo 20-22hr  (751) embryo 22-24hr  (742) larva L1  (1021) larva L2  (722) larva L3 12hr old  (569) larva L3 puffstage 1-2  (286) larva L3 puffstage 3-6  (203) larva L3 puffstage 7-9  (147) white prepupae new    100 white prepupae 12hr  123 white prepupae 24hr    58 pupae 2d postWPP    75 pupae 3d postWPP    85 pupae 4d postWPP  (261) adult male 01day  (259) adult male 05day  (221) adult male 30day  (248) adult female 01day  (427) adult female 05day  (295) adult female 30day  (294) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    252 embryo 02-04hr    51 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    41 embryo 10-12hr    87 embryo 12-14hr    102 embryo 14-16hr    254 embryo 16-18hr    348 embryo 18-20hr    470 embryo 20-22hr    751 embryo 22-24hr    742 larva L1    1021 larva L2    722 larva L3 12hr old    569 larva L3 puffstage 1-2    286 larva L3 puffstage 3-6    203 larva L3 puffstage 7-9    147 white prepupae new    100 white prepupae 12hr    123 white prepupae 24hr    58 pupae 2d postWPP    75 pupae 3d postWPP    85 pupae 4d postWPP    261 adult male 01day    259 adult male 05day    221 adult male 30day    248 adult female 01day    427 adult female 05day    295 adult female 30day    294 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    150 Schneider line 2 Sg4    114 embryonic 1182-4H    226 embryonic GM2    176 embryonic Kc167    75 embryonic S1    180 embryonic S3    172 leg disc CME L1    107 wing disc CME-W2    164 wing disc ML-DmD8    114 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    251 wing disc ML-DmD21    200 wing disc ML-DmD32    126 haltere disc ML-DmD17-c3    173 eye-antennal disc ML-DmD11    235 antennal disc ML-DmD20-c5    268 mixed discs ML-DmD4-c1    120 CNS ML-DmBG1-c1    216 CNS ML-DmBG2-c2    262 tumorous blood cells mbn2    159 ovary fGS/OSS    168 ovary OSC    84 ovary OSS    149 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (150) Schneider line 2 Sg4  (114) embryonic 1182-4H  (226) embryonic GM2  (176) embryonic Kc167  (75) embryonic S1  (180) embryonic S3  (172) leg disc CME L1  (107) wing disc CME-W2  (164) wing disc ML-DmD8  (114) wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (251) wing disc ML-DmD21  (200) wing disc ML-DmD32  (126) haltere disc ML-DmD17-c3  (173) eye-antennal disc ML-DmD11  (235) antennal disc ML-DmD20-c5  (268) mixed discs ML-DmD4-c1  (120) CNS ML-DmBG1-c1  (216) CNS ML-DmBG2-c2  (262) tumorous blood cells mbn2  (159) ovary fGS/OSS  (168) ovary OSC  (84) ovary OSS  (149) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (150) Schneider line 2 Sg4  (114) embryonic 1182-4H  (226) embryonic GM2  (176) embryonic Kc167    75 embryonic S1  (180) embryonic S3  (172) leg disc CME L1  107 wing disc CME-W2  (164) wing disc ML-DmD8  (114) wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (251) wing disc ML-DmD21  (200) wing disc ML-DmD32  (126) haltere disc ML-DmD17-c3  (173) eye-antennal disc ML-DmD11  (235) antennal disc ML-DmD20-c5  (268) mixed discs ML-DmD4-c1  (120) CNS ML-DmBG1-c1  (216) CNS ML-DmBG2-c2  (262) tumorous blood cells mbn2  (159) ovary fGS/OSS  (168) ovary OSC    84 ovary OSS  (149) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    150 Schneider line 2 Sg4    114 embryonic 1182-4H    226 embryonic GM2    176 embryonic Kc167    75 embryonic S1    180 embryonic S3    172 leg disc CME L1    107 wing disc CME-W2    164 wing disc ML-DmD8    114 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    251 wing disc ML-DmD21    200 wing disc ML-DmD32    126 haltere disc ML-DmD17-c3    173 eye-antennal disc ML-DmD11    235 antennal disc ML-DmD20-c5    268 mixed discs ML-DmD4-c1    120 CNS ML-DmBG1-c1    216 CNS ML-DmBG2-c2    262 tumorous blood cells mbn2    159 ovary fGS/OSS    168 ovary OSC    84 ovary OSS    149 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    150 Schneider line 2 Sg4    114 embryonic 1182-4H    226 embryonic GM2    176 embryonic Kc167    75 embryonic S1    180 embryonic S3    172 leg disc CME L1    107 wing disc CME-W2    164 wing disc ML-DmD8    114 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    251 wing disc ML-DmD21    200 wing disc ML-DmD32    126 haltere disc ML-DmD17-c3    173 eye-antennal disc ML-DmD11    235 antennal disc ML-DmD20-c5    268 mixed discs ML-DmD4-c1    120 CNS ML-DmBG1-c1    216 CNS ML-DmBG2-c2    262 tumorous blood cells mbn2    159 ovary fGS/OSS    168 ovary OSC    84 ovary OSS    149 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (150) Schneider line 2 Sg4  (114) embryonic 1182-4H  (226) embryonic GM2  (176) embryonic Kc167  (75) embryonic S1  (180) embryonic S3  (172) leg disc CME L1  (107) wing disc CME-W2  (164) wing disc ML-DmD8  (114) wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (251) wing disc ML-DmD21  (200) wing disc ML-DmD32  (126) haltere disc ML-DmD17-c3  (173) eye-antennal disc ML-DmD11  (235) antennal disc ML-DmD20-c5  (268) mixed discs ML-DmD4-c1  (120) CNS ML-DmBG1-c1  (216) CNS ML-DmBG2-c2  (262) tumorous blood cells mbn2  (159) ovary fGS/OSS  (168) ovary OSC  (84) ovary OSS  (149) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (150) Schneider line 2 Sg4  114 embryonic 1182-4H  (226) embryonic GM2  (176) embryonic Kc167    75 embryonic S1  (180) embryonic S3  (172) leg disc CME L1  107 wing disc CME-W2  (164) wing disc ML-DmD8  114 wing disc ML-DmD9  (179) wing disc ML-DmD16-c3  (251) wing disc ML-DmD21  (200) wing disc ML-DmD32  126 haltere disc ML-DmD17-c3  (173) eye-antennal disc ML-DmD11  (235) antennal disc ML-DmD20-c5  (268) mixed discs ML-DmD4-c1  120 CNS ML-DmBG1-c1  (216) CNS ML-DmBG2-c2  (262) tumorous blood cells mbn2  (159) ovary fGS/OSS  (168) ovary OSC    84 ovary OSS  (149) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    150 Schneider line 2 Sg4    114 embryonic 1182-4H    226 embryonic GM2    176 embryonic Kc167    75 embryonic S1    180 embryonic S3    172 leg disc CME L1    107 wing disc CME-W2    164 wing disc ML-DmD8    114 wing disc ML-DmD9    179 wing disc ML-DmD16-c3    251 wing disc ML-DmD21    200 wing disc ML-DmD32    126 haltere disc ML-DmD17-c3    173 eye-antennal disc ML-DmD11    235 antennal disc ML-DmD20-c5    268 mixed discs ML-DmD4-c1    120 CNS ML-DmBG1-c1    216 CNS ML-DmBG2-c2    262 tumorous blood cells mbn2    159 ovary fGS/OSS    168 ovary OSC    84 ovary OSS    149 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    155 cold shock, 4-day adult    124 heat shock, 4-day adult    295 Cadmium 50 mM 6 hrs, larvae L3    304 Cadmium 50 mM 12 hrs, larvae L3    206 Cadmium 50 mM 48 hrs, 4-day adult    349 Cadmium 100 mM 48 hrs, 4-day adult    219 Copper 0.5 mM 12 hrs, larvae L3    248 Copper 15 mM 48 hrs, 4-day adult    232 Zinc 5 mM 12 hrs, larvae L3    258 Zinc 4.5 mM 48 hrs, 4-day adult    249 Ethanol 2.5% 3 hrs, larvae L3    275 Ethanol 5% 3 hrs, larvae L3    408 Ethanol 10% 3 hrs, larvae L3    401 Caffeine 1.5 mg/ml 4 hrs, larvae L3    329 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    287 Caffeine 25 mg/ml 48 hrs, 4-day adult    158 Paraquat 5 mM 48 hrs, 4-day adult    284 Paraquat 10 mM 48 hrs, 4-day adult    306 Rotenone 2 μg 12 hrs, larvae L3    365 Rotenone 8 μg 12 hrs, larvae L3    423 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (155) cold shock, 4-day adult  (124) heat shock, 4-day adult  (295) Cadmium 50 mM 6 hrs, larvae L3  (304) Cadmium 50 mM 12 hrs, larvae L3  (206) Cadmium 50 mM 48 hrs, 4-day adult  (349) Cadmium 100 mM 48 hrs, 4-day adult  (219) Copper 0.5 mM 12 hrs, larvae L3  (248) Copper 15 mM 48 hrs, 4-day adult  (232) Zinc 5 mM 12 hrs, larvae L3  (258) Zinc 4.5 mM 48 hrs, 4-day adult  (249) Ethanol 2.5% 3 hrs, larvae L3  (275) Ethanol 5% 3 hrs, larvae L3  (408) Ethanol 10% 3 hrs, larvae L3  (401) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (329) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (287) Caffeine 25 mg/ml 48 hrs, 4-day adult  (158) Paraquat 5 mM 48 hrs, 4-day adult  (284) Paraquat 10 mM 48 hrs, 4-day adult  (306) Rotenone 2 μg 12 hrs, larvae L3  (365) Rotenone 8 μg 12 hrs, larvae L3  (423) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (155) cold shock, 4-day adult  (124) heat shock, 4-day adult  (295) Cadmium 50 mM 6 hrs, larvae L3  (304) Cadmium 50 mM 12 hrs, larvae L3  (206) Cadmium 50 mM 48 hrs, 4-day adult  (349) Cadmium 100 mM 48 hrs, 4-day adult  (219) Copper 0.5 mM 12 hrs, larvae L3  (248) Copper 15 mM 48 hrs, 4-day adult  (232) Zinc 5 mM 12 hrs, larvae L3  (258) Zinc 4.5 mM 48 hrs, 4-day adult  (249) Ethanol 2.5% 3 hrs, larvae L3  (275) Ethanol 5% 3 hrs, larvae L3  (408) Ethanol 10% 3 hrs, larvae L3  (401) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (329) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (287) Caffeine 25 mg/ml 48 hrs, 4-day adult  (158) Paraquat 5 mM 48 hrs, 4-day adult  (284) Paraquat 10 mM 48 hrs, 4-day adult  (306) Rotenone 2 μg 12 hrs, larvae L3  (365) Rotenone 8 μg 12 hrs, larvae L3  (423) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    155 cold shock, 4-day adult    124 heat shock, 4-day adult    295 Cadmium 50 mM 6 hrs, larvae L3    304 Cadmium 50 mM 12 hrs, larvae L3    206 Cadmium 50 mM 48 hrs, 4-day adult    349 Cadmium 100 mM 48 hrs, 4-day adult    219 Copper 0.5 mM 12 hrs, larvae L3    248 Copper 15 mM 48 hrs, 4-day adult    232 Zinc 5 mM 12 hrs, larvae L3    258 Zinc 4.5 mM 48 hrs, 4-day adult    249 Ethanol 2.5% 3 hrs, larvae L3    275 Ethanol 5% 3 hrs, larvae L3    408 Ethanol 10% 3 hrs, larvae L3    401 Caffeine 1.5 mg/ml 4 hrs, larvae L3    329 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    287 Caffeine 25 mg/ml 48 hrs, 4-day adult    158 Paraquat 5 mM 48 hrs, 4-day adult    284 Paraquat 10 mM 48 hrs, 4-day adult    306 Rotenone 2 μg 12 hrs, larvae L3    365 Rotenone 8 μg 12 hrs, larvae L3    423 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    155 cold shock, 4-day adult    124 heat shock, 4-day adult    295 Cadmium 50 mM 6 hrs, larvae L3    304 Cadmium 50 mM 12 hrs, larvae L3    206 Cadmium 50 mM 48 hrs, 4-day adult    349 Cadmium 100 mM 48 hrs, 4-day adult    219 Copper 0.5 mM 12 hrs, larvae L3    248 Copper 15 mM 48 hrs, 4-day adult    232 Zinc 5 mM 12 hrs, larvae L3    258 Zinc 4.5 mM 48 hrs, 4-day adult    249 Ethanol 2.5% 3 hrs, larvae L3    275 Ethanol 5% 3 hrs, larvae L3    408 Ethanol 10% 3 hrs, larvae L3    401 Caffeine 1.5 mg/ml 4 hrs, larvae L3    329 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    287 Caffeine 25 mg/ml 48 hrs, 4-day adult    158 Paraquat 5 mM 48 hrs, 4-day adult    284 Paraquat 10 mM 48 hrs, 4-day adult    306 Rotenone 2 μg 12 hrs, larvae L3    365 Rotenone 8 μg 12 hrs, larvae L3    423 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (155) cold shock, 4-day adult  (124) heat shock, 4-day adult  (295) Cadmium 50 mM 6 hrs, larvae L3  (304) Cadmium 50 mM 12 hrs, larvae L3  (206) Cadmium 50 mM 48 hrs, 4-day adult  (349) Cadmium 100 mM 48 hrs, 4-day adult  (219) Copper 0.5 mM 12 hrs, larvae L3  (248) Copper 15 mM 48 hrs, 4-day adult  (232) Zinc 5 mM 12 hrs, larvae L3  (258) Zinc 4.5 mM 48 hrs, 4-day adult  (249) Ethanol 2.5% 3 hrs, larvae L3  (275) Ethanol 5% 3 hrs, larvae L3  (408) Ethanol 10% 3 hrs, larvae L3  (401) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (329) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (287) Caffeine 25 mg/ml 48 hrs, 4-day adult  (158) Paraquat 5 mM 48 hrs, 4-day adult  (284) Paraquat 10 mM 48 hrs, 4-day adult  (306) Rotenone 2 μg 12 hrs, larvae L3  (365) Rotenone 8 μg 12 hrs, larvae L3  (423) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (155) cold shock, 4-day adult  124 heat shock, 4-day adult  (295) Cadmium 50 mM 6 hrs, larvae L3  (304) Cadmium 50 mM 12 hrs, larvae L3  (206) Cadmium 50 mM 48 hrs, 4-day adult  (349) Cadmium 100 mM 48 hrs, 4-day adult  (219) Copper 0.5 mM 12 hrs, larvae L3  (248) Copper 15 mM 48 hrs, 4-day adult  (232) Zinc 5 mM 12 hrs, larvae L3  (258) Zinc 4.5 mM 48 hrs, 4-day adult  (249) Ethanol 2.5% 3 hrs, larvae L3  (275) Ethanol 5% 3 hrs, larvae L3  (408) Ethanol 10% 3 hrs, larvae L3  (401) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (329) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (287) Caffeine 25 mg/ml 48 hrs, 4-day adult  (158) Paraquat 5 mM 48 hrs, 4-day adult  (284) Paraquat 10 mM 48 hrs, 4-day adult  (306) Rotenone 2 μg 12 hrs, larvae L3  (365) Rotenone 8 μg 12 hrs, larvae L3  (423) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    155 cold shock, 4-day adult    124 heat shock, 4-day adult    295 Cadmium 50 mM 6 hrs, larvae L3    304 Cadmium 50 mM 12 hrs, larvae L3    206 Cadmium 50 mM 48 hrs, 4-day adult    349 Cadmium 100 mM 48 hrs, 4-day adult    219 Copper 0.5 mM 12 hrs, larvae L3    248 Copper 15 mM 48 hrs, 4-day adult    232 Zinc 5 mM 12 hrs, larvae L3    258 Zinc 4.5 mM 48 hrs, 4-day adult    249 Ethanol 2.5% 3 hrs, larvae L3    275 Ethanol 5% 3 hrs, larvae L3    408 Ethanol 10% 3 hrs, larvae L3    401 Caffeine 1.5 mg/ml 4 hrs, larvae L3    329 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    287 Caffeine 25 mg/ml 48 hrs, 4-day adult    158 Paraquat 5 mM 48 hrs, 4-day adult    284 Paraquat 10 mM 48 hrs, 4-day adult    306 Rotenone 2 μg 12 hrs, larvae L3    365 Rotenone 8 μg 12 hrs, larvae L3    423 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	BDGP expression data • RE30552
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Vha68-201510 Vha68-2R6 lethal | first instar larval stage Vha68-2P67 Vha68-2S6 lethal | pupal stage, with Scer\GAL4pnr-MD237 Vha68-2GD10785 lethal | recessive Vha68-201510 Vha68-207007 Vha68-2k02508 Vha68-2k07404 Vha68-2s4214 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Vha68-2GD10785 Other Phenotypes Allele decreased cell number | larval stage | somatic clone Vha68-2R6 size defective | larval stage | somatic clone Vha68-2R6 visible, with Scer\GAL4pnr-MD237 Vha68-2GD10785 Phenotype manifest in Allele embryonic/larval Malpighian tubule Vha68-2unspecified endosome | larval stage | somatic clone Vha68-2R6 eye disc | somatic clone Vha68-2R6 follicle cell | somatic clone Vha68-2R6 Malpighian tubule Vha68-2P67 Vha68-2S6 mesothoracic tergum, with Scer\GAL4pnr-MD237 Vha68-2GD10785 multivesicular body | larval stage | somatic clone Vha68-2R6
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of Vha68-2 Class Mutagen Stocks Known lesion Vha68-2EP2364 P-element activity 2 -- Vha68-2s4214 P-element activity 2 -- Vha68-201510 P-element activity 1 -- Vha68-25-SZ-3324 1 -- Vha68-2d04859 1 Yes Vha68-2f06851 piggyBac transposase 1 -- Vha68-2MB05049 1 -- Vha68-2R6 ethyl methanesulfonate 1 Yes Vha68-207007 P-element activity 0 -- Vha68-257 0 -- Vha68-2CB-0356-3 0 -- Vha68-2d06866 P-element activity 0 -- Vha68-2GS-A1548 Delta2-3 0 -- Vha68-2k02508 P-element activity 0 -- Vha68-2k07404 P-element activity 0 -- Vha68-2P67 0 -- Vha68-2S6 0 -- Vha68-2unspecified 0 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Vha68-2 Class Mutagen Stocks Known lesion Vha68-2GD10785 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Vha68-2HMS01056 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Vha68-2GD17817 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Vha68-2KK108767 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)BSC245 (Christensen and Cook, 2006.12.5) Df(2L)BSC277 (Christensen and Cook, 2007.5.8) Df(2L)BSC290 (Christensen and Cook, 2007.5.8) Df(2L)BSC344 (Christensen et al., 2007.10.29) Df(2L)BSC855 (Christensen et al., 2009.7.22) Df(2L)Exel7055 (Ryder, 2004.4.26) Df(2L)prd1.7 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2L)Prl (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10785} NA P{GD17817} NA P{KK108767} NA P{TRiP.HMS01056} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Vha68-2EP2364 NA P{GSV1}Vha68-2GS-A1548 NA P{XP}Vha68-2d04859 NA P{XP}Vha68-2d06866 NA insertion of enhancer trap P{lacW}Vha68-2k02508 No P{lacW}Vha68-2k07404 No P{lacW}Vha68-2s4214 No P{lacZ}Vha68-2P67 No P{PZ}Vha68-201510 No P{PZ}Vha68-207007 No miscellaneous insertions P{PT1}Vha68-257 NA P{RS3}Vha68-2CB-0356-3 NA P{RS5}Vha68-25-SZ-3324 NA PBac{WH}Vha68-2f06851 NA insertion of enhancer trap binary system Mi{ET1}Vha68-2MB05049 No
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
endosomal lumen acidification inferred from mutant phenotype (Vaccari et al., 2010) endosomal transport inferred from mutant phenotype (Hermle et al., 2013) imaginal disc growth inferred from mutant phenotype (Vaccari et al., 2010)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Khanna et al., 2010) plasma membrane proton-transporting V-type ATPase complex inferred from mutant phenotype (Allan et al., 2005)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000194, InterPro:IPR020003 (FlyBase Curators et al., 2004-) proton-transporting ATPase activity, rotational mechanism inferred from electronic annotation with InterPro:IPR004100, InterPro:IPR005725, InterPro:IPR018118 (FlyBase Curators et al., 2004-) contributes_to proton-transporting ATPase activity, rotational mechanism inferred by curator from GO:0033181 (Allan et al., 2005)
Biological Process
CV term Evidence References
ATP hydrolysis coupled proton transport inferred by curator from GO:0033181 (Allan et al., 2005) inferred from electronic annotation with InterPro:IPR000793, InterPro:IPR005725 (FlyBase Curators et al., 2004-) ATP metabolic process inferred from electronic annotation with InterPro:IPR004100, InterPro:IPR018118, InterPro:IPR022878 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
vacuolar proton-transporting V-type ATPase, V1 domain inferred from sequence or structural similarity with Saccharomyces TFP1 (Dow, 1999) non-traceable author statement (Dow et al., 1997, Swiss-Prot Project Members, 2000.10.1)
Sequence Ontology: Class of Gene
	gene (Menut et al., 2007) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Vha68-2 - 5-HT7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Aats-his anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Ada anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Atpα experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Bsg anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG10209 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG10581 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG1105 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG12171 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG12262 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - CG12344 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG1246 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG13532 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG14024 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG1444 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - CG15643 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG2789 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG2921 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG2931 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG31522 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG32698 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG33145 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG33303 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - CG33523 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG3509 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG3523 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - CG3530 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG3570 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG3793 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - CG4091 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG4658 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG5027 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG5646 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG7053 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG7277 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vha68-2 - CG7289 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG7655 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG7881 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG8026 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG8888 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vha68-2 - CG9231 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - CG9890 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Ca-P60A experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Cct2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vha68-2 - Chc experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Chi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Ctr1B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Cyp308a1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Cyp6a22 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Fatp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Gfat2 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Gie anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Gmd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Gp93 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Gαo anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Hel25E experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Hip1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Karyβ3 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Lapsyn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - MFS3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Mec2 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Ost48 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Past1 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Pis anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Prosβ2R2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Rab11 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Rab5 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vha68-2 - Rheb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Rpp30 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Sucb experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Tcp-1ζ experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Tcp-1η experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Vap-33-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - Vha14-1 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Vha26 experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - Vha36-1 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vha68-2 - Vha44 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vha68-2 - Vha55 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vha68-2 - VhaSFD experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vha68-2 - aur anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - igl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - nemy anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - nwk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - olf186-F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - p24-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - porin experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - tmod experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - tyn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vha68-2 - wal experimental knowledge based (Guruharsha et al., 2011) Vha68-2 - β4GalNAcTB anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Vha68-2 allele Gene References unspecified ct (Krupp et al., 2005)
External Data
Linkouts
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6DBV05 Dipteran inclusive ortholog search EOG6MGSGK Insect inclusive ortholog search EOG6K3JRB Arthropod inclusive ortholog search EOG6CRJF3 Metazoa inclusive ortholog search EOG6FFRTT
Orthologs in Drosophila Species (EOG6DBV05)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Vha68-2     Drosophila sechellia   Dsec\GM10085     Drosophila erecta   Dere\GG23804     Drosophila yakuba   Dyak\GE18610     Drosophila ananassae   Dana\GF14214     Drosophila pseudoobscura pseudoobscura   Dpse\GA28953     Drosophila willistoni   Dwil\GK15307 Y   Drosophila virilis   Dvir\GJ16804 Y   Drosophila mojavensis   Dmoj\GI18088     Drosophila grimshawi   Dgri\GH11385
Orthologs in non-Drosophila Dipterans (EOG6MGSGK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\VhaA   Anopheles gambiae Malaria mosquito  Agam\VATA_ANOGA   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ007772
Orthologs in non-Dipteran Insects (EOG6K3JRB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC551093 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG001459 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\atpA Y Atta cephalotes Leafcutter ant  Acep\atpA Y Camponotus floridanus Florida carpenter ant  Cflo\atpA Y Harpegnathos saltator Jerdons jumping ant  Hsal\atpA Y Linepithema humile Argentine ant  Lhum\LH22479 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB20547 Y Solenopsis invicta Red fire ant  Sinv\SINV10715 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI002584 Y Bombyx mori Silkmoth  Bmor\BGIBMGA008295 Y Pediculus humanus Human body louse  Phum\PHUM288180 Y Tribolium castaneum Red flour beetle  Tcas\NtpA Y
Orthologs in non-Insect Arthropods (EOG6CRJF3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Eif3s6ip Y Ixodes scapularis Deer tick  Isca\ISCW002559 Y
Orthologs in non-Arthropod Metazoa (EOG6FFRTT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\vha-13 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016882gn Y Danio rerio Zebrafish  Drer\ENSDARG00000076318 Y Danio rerio Zebrafish  Drer\atp6v1al Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000000901 Y Xenopus tropicalis Western clawed frog  Xtro\atp6v1a Y Gallus gallus Domestic chicken  Ggal\ATP6V1A Y Mus musculus House mouse  Mmus\Atp6v1a Y Rattus norvegicus Norway rat  Rnor\Atp6v1a Y Homo sapiens Human  Hsap\ATP6V1A Y
Human Orthologs (1)
Gene OMIM HGNC Hsap\ATP6V1A
AAA Orthologs (2) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila willistoni Dwil\GK15307 Drosophila virilis Dvir\GJ16804
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Bloomington	24318 w1118; Mi{ET1}Vha68-2MB05049 17243 w1118; P{EP}Vha68-2EP2364/CyO 10463 w1118; P{lacW}Vha68-2s4214/CyO 11065 P{PZ}Vha68-201510 cn1/CyO; ry506 34582 y1 sc* v1; P{TRiP.HMS01056}attP2 39621 w*; Vha68-2R6 P{neoFRT}40A/CyO, P{GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2
Harvard	- P{XP}Vha68-2d04859 - PBac{WH}Vha68-2f06851
Kyoto	125993 w1118; P{RS5}Vha68-25-SZ-3324 114463 yd2 w1118 P{ey-FLP.N}2; P{EP}Vha68-2EP2364 P{neoFRT}40A/CyO y+
VDRC	v34389 w1118; P{GD10785}v34389 v34390 w1118; P{GD10785}v34390
	More stocks available...
Genomic Clones ( 2 )
	BACR11M13 BACR48D10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 242 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH21132 RE30552
Other clones	MIP16230
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT04012 AT13946 AT14888 GH02521 GH02935 GH04708 GH06628 GH07563 GH09208 GH10107 GH10545 GH10636 GH12532 GM02748 GM06074 GM06562 GM09109 LD07081 LD07858 LD08202 LD13156 LD14081 LD14733 LD15326 LD15843 LD20450 LD33190 LD41505 LD44536 LP04948 LP13493 LP15609 LP19112 LP21347 RE02846 RE15628 RE17302 RE26109 RE27729 RE31785 RE32150 RE35808 RE47577 RE56110 RE64306 RE64455 RE69430 RE69534 RE71759 RE74623 RE74764 RH05271 RH05740 RH07957 RH15121 RH16839 RH20205 RH23152 RH25445 RH27485 RH30750 RH30752 RH33208 RH35817 RH36269 RH36429 RH36691 RH37052 RH44919 RH49107 RH66767 RH71907 SD01367 SD05366 SD06952 SD11160 SD11320
Other clones	50935 53917 56395 66749 84389 102656 103012 107444 147667 166255 166861 192261 274735 274876 572802 CK01.23D.G1 CK01.24D.G9 CK01.126C.D3 CK01.127D.H11 Dmel_dig_009494_0621_1848 Dmel_dig_010953_0694_1923 Dmel_dig_019475_0642_1174 Dmel_dig_019862_0666_3495 Dmel_neb_011754_0934_3178 Dmel_neb_012188_0107_0759 Dmel_neb_012332_0625_2066 Dmel_neb_012924_0573_2077 Dmel_neb_014226_0327_1806 Dmel_neb_014253_0626_3333 Dmel_neb_014584_0081_2019 Dmel_neb_015277_0705_0410 EC40863 EK043943 EK097014 EK098624 EK169056 EK177241 EK185105 EK208109 EK218915 EK224351 EK225737 EK242710 EK252961 EK271860 EK295412 EK302327 EK302637 EK307801 EN17113 ESG013a.E21.1_A05 ESG015c.E21.1_D07 ESG019c.E21_C01 FGH120D01 FGM301G10 RE01683 RP003022613 RP003026848 RP003033951 RP003047960 RP003048571 RP003049484 RP003058654 RP003059780 RP003066860 RP003068682 RP003073453 RP003076352 RP003082490 RP003085279 RP003086693 RP003087235 RP003091972 RP003095433 RP003097428 RP003099519 RP003100069 RP003106522 RP003108799 RP003118036 RP003118568 RP003131989 RP003137292 RP003142065 RP003143405 RP003146036 RP003146445 RP003147061 RP003150367 RP003152511 RP003158702 RP003167334 RP003178767 RP003179086 RP003180816 RP003185250 RP003188126 RP003188689 RP003192846 RP003194033 RP003199696 RP003200916 RP003203671 RP003209133 RP003215524 RP003224235 RP003229486 RP003234355 RP003238488 RP003242243 RP003247676 RP003248941 RP003261111 RP003272709 RP003283785 RP003289060 RP003292292 RP003295748 RP003300883 RP003302105 RP003303794 RP003311685 RP003321267 RP003327167 RP003339289 RP003348749 RP003349776 RP003350490 RP003358647 RP003371409 RP003382963 RP003391432 RP003414850 RP003420226 RP003424147 RP003424835 RP003439407 RP003445195 RP003451898 RP003452953 RP003462262 RP003471976 RP003478029 RP003479097 RP003481217 RP003496341 RP003515195 RP003530358 RP003535301 RP003548030 RP003555717 RP003585204 RP003588673 RP003592592 RP003618891 RP003628192 RP003629465 RP003634426 RP003657826 RP003673033 RP003675288 UUGC0653
RNAi & Array Information
Linkouts	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45012
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Vha68-2 CG3762 (Misra, 2000.8.9) Source for merge of: Vha68-2 MENE-R6 (Vaccari et al., 2010)
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143747 NM_165021 NM_165022 NM_001259086 NM_001273495 NM_001273496 RefSeq (Proteins) • NP_652004 NP_723775 NP_723776 NP_001246015 NP_001260424 NP_001260425 DDBJ / EMBL / GenBank • AAB02270 AAB02271 AAF53231 AAF53232 AAF53233 AAL89888 AFH03689 AGB92959 AGB92960 AQ073265 AQ254852 AY084150 BI234795 CZ475403 CZ489581 U59146 U59147 Entrez Gene - A searchable database of RefSeq genes. • 45012 UniProtKB/Swiss-Prot • Q27331 UniProtKB/TrEMBL • A4V0N4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • ATPase, F1 complex beta subunit/V1 complex, C-terminal (IPR024034) ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain (IPR000194) ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal (IPR000793) ATPase, alpha/beta subunit, N-terminal (IPR004100) ATPase, V1 complex, subunit A (IPR005725) ATPase, alpha/beta subunit, nucleotide-binding domain, active site (IPR020003) V-type ATP synthase catalytic subunit A/alpha (IPR022878) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE30552
Linkouts
	GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45012
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	A subunit (Dow et al., 1997) ATP6V1A (Adamson et al., 2011) CG3762 (Cammarato et al., 2011, Keleman et al., 2009.8.5, Ni et al., 2010.12.1, Wang et al., 2004, Terhzaz et al., 2006, Allan et al., 2005, Chintapalli et al., 2007, Lai et al., 2007, Khanna et al., 2010, Vaccari et al., 2010) l(2)01510   l(2)s4214   MENE-R6   vha67-2 (Dow, 1999) Vha68 (Larsen et al., 2006, Guo et al., 1996) vha68-2 (Guo, 1996.5.23, Guo, 1996.5.23, Sozen et al., 1997, Dow et al., 1997, Dow et al., 1997, Guo et al., 1996, Stark et al., 2005, Wang et al., 2004, Terhzaz et al., 2006, Day et al., 2008, Allan et al., 2005, Adamson et al., 2011) Vha68-2 (Mack et al., 2006, Christensen and Cook, 2007.5.8, Christensen and Cook, 2006.12.5, Christensen and Cook, 2007.5.8, Christensen et al., 2007.10.29, Dorus et al., 2006, Christensen et al., 2009.7.22, Shalaby et al., 2009, Vaccari et al., 2010, Rees et al., 2011, Hermle et al., 2013, Abruzzi et al., 2011) Vha68.2 (Koles et al., 2012)
Name Synonym	vacuolar ATPase (Vaccari et al., 2010) Vacuolar H+ ATPase 68 kDa subunit 2   Vacuolar H+ ATPase subunit 68-2   V-ATPase 69 kDa subunit 2 (Khanna et al., 2010, Vaccari et al., 2010) Vha68-2 (Cammarato et al., 2011, )
Secondary FlyBase IDs
	FBgn0010494 FBgn0010694 FBgn0020367 FBgn0032463 FBgn0260570
References ( 68 )
Generate a list of	All references relating to this gene ( 68 )
List References by type	Research paper  ( 24 ) Supplementary material  ( 10 ) Personal communication to FlyBase  ( 21 ) Abstract  ( 2 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 3 )
Recent research papers ( 5 )
	Hermle et al., 2013, EMBO J. 32(2): 245--259 Drosophila ATP6AP2/VhaPRR functions both as a novel planar cell polarity core protein and a regulator of endosomal trafficking. [FBrf0220613] Koles et al., 2012, J. Biol. Chem. 287(20): 16820--16834 Mechanism of evenness interrupted (evi)-exosome release at synaptic boutons. [FBrf0218323] Abruzzi et al., 2011, Genes Dev. 25(22): 2374--2386 Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. [FBrf0216798] Adamson et al., 2011, Genetics 189(2): 495--506 A Drosophila model for genetic analysis of influenza viral/host interactions. [FBrf0216397] Rees et al., 2011, Mol. Cell. Proteomics 10(6): M110.002386 In Vivo Analysis of Proteomes and Interactomes Using Parallel Affinity Capture (iPAC) Coupled to Mass Spectrometry. [FBrf0213845] Show all research papers ...