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Gene Dmel\l(3)72Ab

General Information
Symbol	Dmel\l(3)72Ab	Species	D. melanogaster
Name	lethal (3) 72Ab	Annotation symbol	CG5931
Feature type	protein_coding_gene	FlyBase ID	FBgn0263599
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	Brr2
Genomic Location
Chromosome (arm)	3L	Recombination map	3-[43]
Cytogenetic map	72C1-72C1	Sequence location	3L:15,953,908..15,960,956 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene lethal (3) 72Ab is referred to in FlyBase by the symbol Dmel\l(3)72Ab (CG5931, FBgn0263599). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATP-dependent RNA helicase activity; RNA helicase activity. There is experimental evidence that it is involved in the biological process: mitosis; regulation of alternative nuclear mRNA splicing, via spliceosome. 16 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: DEAD-like helicase; DNA/RNA helicase, DEAD/DEAH box type, N-terminal; Helicase, C-terminal; Sec63 domain. Gene sequence location is 3L:15953908..15960956.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Sequence features HFA10559 BKN20752
FB2011_10	Controlled Vocabulary Terms day 42 of adulthood day 41 of adulthood day 30 of adulthood day 49 of adulthood day 17 of adulthood day 45 of adulthood day 6 of adulthood day 5 of adulthood day 8 of adulthood day 26 of adulthood day 21 of adulthood day 13 of adulthood day 25 of adulthood day 22 of adulthood day 20 of adulthood day 39 of adulthood day 7 of adulthood day 36 of adulthood day 11 of adulthood day 37 of adulthood day 27 of adulthood day 18 of adulthood day 19 of adulthood day 28 of adulthood day 9 of adulthood day 29 of adulthood adult heart day 40 of adulthood day 16 of adulthood day 24 of adulthood day 15 of adulthood day 33 of adulthood day 43 of adulthood day 47 of adulthood day 38 of adulthood day 32 of adulthood day 48 of adulthood day 35 of adulthood day 23 of adulthood day 12 of adulthood day 10 of adulthood day 31 of adulthood spermatozoon day 34 of adulthood day 44 of adulthood day 14 of adulthood male day 46 of adulthood
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 72C1-72C1   Limits computationally determined from genome sequence between P{EP}Tfb2EP572 and P{lacW}thj5C8&P{PZ}Mbs03802
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	3-[43]
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\l(3)72Ab for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) l(3)72Ab-RA FBtr0075527  NM_140561   6719   2142
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein l(3)72Ab-PA   FBpp0075282   244.5   2142   5.91     NP_648818   AAF49564
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Helicase, C-terminal (IPR001650) Sec63 domain (IPR004179) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) DEAD-like helicase (IPR014001)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201447   AA816680   AA820691   AA821079   AE014296 AAF49564   AI064318   AI124259   AI238158   AI455495   AI544244   AW941342   AY069270 AAL39415   BG632171   BI632413   BT016121 AAV37006   BT029277   CK658792   CO189719   CO193860   CO263539   CO264406   CO269381   CO274134   CO275222   CO282463   CO291702   CO305382   CO310627   CO331134   CO339422   CO341790   CZ469801   CZ480935   CZ480936   CZ482803   CZ485111   EC052088   EC053594   EC054069   EC055123   EC055612   EC056187   EC056602   EC062110   EC065933   EC071168   EC076087   EC195444   EC221265   EC231333   EC235447   EC242762   EC251481   EC252995   GH974279   GH978854
UniProtKB/Swiss-Prot	Q9VUV9
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. [an error occurred while processing this directive] modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | recessive l(3)72AbI24 lethal | recessive l(3)72Ab1 l(3)72Ab2 l(3)72Ab3 l(3)72Ab4 l(3)72Ab5 l(3)72Ab6 viable, with Scer\GAL4pnr-MD237 l(3)72AbGD9881 Phenotype manifest in Allele eye | maternal effect (with l(3)72Ab3) l(3)72Ab4 eye | maternal effect (with l(3)72Ab4) l(3)72Ab3 wing | maternal effect (with l(3)72Ab3) l(3)72Ab4 wing | maternal effect (with l(3)72Ab4) l(3)72Ab3
Classical Alleles ( 11 )
For All Classical Alleles Show
Allele of l(3)72Ab Class Mutagen Stocks Known lesion l(3)72Abe03171 piggyBac transposase 2 -- l(3)72Abf00335 piggyBac transposase 1 -- l(3)72Abf02582 piggyBac transposase 1 -- l(3)72AbI24 1 -- l(3)72Ab1 ethyl methanesulfonate 0 -- l(3)72Ab2 ethyl methanesulfonate 0 -- l(3)72Ab3 ethyl methanesulfonate 0 -- l(3)72Ab4 ethyl methanesulfonate 0 -- l(3)72Ab5 ethyl methanesulfonate 0 -- l(3)72Ab6 ethyl methanesulfonate 0 -- l(3)72Abc05233 piggyBac transposase 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of l(3)72Ab Class Mutagen Stocks Known lesion l(3)72AbGD9881 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes l(3)72AbHMS00994 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes l(3)72AbJF01149 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes l(3)72AbdsRNA.cRa in vitro construct - RNAi 0 Yes l(3)72AbKK100656 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(3L)A5 (Melendez et al., 1995) Df(3L)BK10 (Melendez et al., 1995) Df(3L)Brd6 (Melendez et al., 1995) Df(3L)Brd20 (Melendez et al., 1995) Df(3L)st-f13 (Brizuela et al., 1994, Melendez et al., 1995) Df(3L)th117 (Melendez et al., 1995)
Disrupted in	Df(3L)brm11 (Brizuela et al., 1994, Melendez et al., 1995, Cooper and Kennison, 2011) Df(3L)BSC443 (Cooper and Kennison, 2011) Df(3L)BSC575 (Cooper and Kennison, 2011) Df(3L)BSC774 (Cooper and Kennison, 2011) Df(3L)Q26 (Melendez et al., 1995) Df(3L)th102 (Brizuela et al., 1994, Cooper and Kennison, 2011) Df(3L)X-21.2 (Cooper and Kennison, 2011) Df(3L)XG5 (Cooper and Kennison, 2011)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}l(3)72Abc05233 NA PBac{RB}l(3)72Abe03171 NA PBac{WH}l(3)72Abf00335 NA PBac{WH}l(3)72Abf02582 NA
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mitosis inferred from mutant phenotype (Ducat et al., 2008) regulation of alternative nuclear mRNA splicing, via spliceosome inferred from mutant phenotype (Park et al., 2004, Katzenberger et al., 2009)
Cellular Component
CV term Evidence References
catalytic step 2 spliceosome inferred from direct assay (Herold et al., 2009) precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Terms Based on Predictions or Assertions ( 10 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-) ATP-dependent RNA helicase activity inferred from sequence or structural similarity with Saccharomyces BRR2 (Lasko, 2000) inferred from sequence or structural similarity with UniProtKB:O75643 (assigned by UniProtKB) (Swiss-Prot Project Members, 2003.9.15) nucleic acid binding inferred from electronic annotation with InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-) RNA helicase activity inferred from sequence or structural similarity (Liang and Biggin, 1998)
Biological Process
CV term Evidence References
nuclear mRNA splicing, via spliceosome inferred by curator from GO:0071011|GO:0071013 (Herold et al., 2009) RNA splicing inferred from sequence or structural similarity (Liang and Biggin, 1998) inferred from sequence or structural similarity with UniProtKB:O75643 (assigned by UniProtKB) (Swiss-Prot Project Members, 2003.9.15)
Cellular Component
CV term Evidence References
nucleus inferred by curator from GO:0000381 (Park et al., 2004) small nuclear ribonucleoprotein complex inferred from sequence or structural similarity with EMBL:Z70200 (FlyBase, 1992-) spliceosomal complex inferred from sequence or structural similarity with UniProtKB:O75643 (assigned by UniProtKB) (Swiss-Prot Project Members, 2003.9.15) U5 snRNP inferred from sequence or structural similarity with EMBL:Z70200 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions l(3)72Ab allele Gene References
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs	Dana\GF10367 Dere\GG13510 Dgri\GH16760 Dmoj\GI13421 Dpse\GA19239 Dvir\GJ11781 Dwil\GK23638 Dyak\GE19803
Curated drosophilid orthologs	Dpse\GA19239
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	31576 y1 v1; P{TRiP.JF01149}attP2 34024 y1 sc* v1; P{TRiP.HMS00994}attP2 18127 w1118; PBac{RB}l(3)72Abe03171/TM6B, Tb1 4109 w1118; l(3)72AbI24 P{white-un4}71F/TM3, Sb1
Harvard	- PBac{RB}l(3)72Abe03171 - PBac{WH}l(3)72Abf00335 - PBac{WH}l(3)72Abf02582
VDRC	v43962 w1118; P{GD9881}v43962
Genomic Clones ( 1 )
	BACR18K24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 45 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM09620 IP09935 LD03265
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH04577 GH04690 GH14159 LD04475 LP16036 SD10732 SD26177
Other clones	30117 45160 70283 99527 145890 214345.52 214563.52 AI124259 CK01.55B.F6 CK01.106B.F2 CK01.123D.E6 EC36913 EC052088 EC053594 EC054069 EC055123 EC055612 EC056187 EC056602 EK044955 EK088753 EK094251 EK113302 EK116808 EK138960 EK160535 EK198017 EK222154 EK248416 EK298461 EP01945 EP13146 LD24541 RP004256198 RP004366802
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG5931 anon-EST:Liang-1.21 (Ashburner, 2004.6.30) Source for merge of: l(3)72Ab CG5931 (Cooper and Kennison, 2011)
Additional comments
Other Comments
	The l(3)72Ab complementation group comprises 17 EMS-induced mutant alleles. (Cooper and Kennison, 2011) Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells. (Nybakken et al., 2005)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140561 RefSeq (Proteins) • NP_648818 DDBJ / EMBL / GenBank • AA816680 AA821079 AAF49564 AAL39415 AAV37006 AI124259 AW941342 AY069270 BT016121 CZ469801 CZ480935 CZ480936 CZ482803 CZ485111 Entrez Gene - A searchable database of RefSeq genes. • 39737 UniProtKB/Swiss-Prot • Q9VUV9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Helicase, C-terminal (IPR001650) Sec63 domain (IPR004179) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) DEAD-like helicase (IPR014001)
Linkouts
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	anon-EST:Liang-1.21   Brr2 (Park et al., 2004, Katzenberger et al., 2009) CG5931 (Cooper and Kennison, 2011, Cammarato et al., 2011, Keleman et al., 2009.8.5, Wasbrough et al., 2010, Ni et al., 2010.12.1, Nybakken et al., 2005, Farny et al., 2008, Ducat et al., 2008, Katzenberger et al., 2009, Andersen and Tapon, 2008, Herold et al., 2009, Ni et al., 2008) cg5931 (Lasko, 2000) clone 1.21 (Liang and Biggin, 1998) l(3)72Ab (Cooper and Kennison, 2011, )
Name Synonym	lethal (3) 72Ab
Secondary FlyBase IDs
	FBgn0004713 FBgn0026003 FBgn0036548
References ( 38 )
Generate a list of	All references relating to this gene ( 38 )
List References by type	Research paper  ( 16 ) Supplementary material  ( 4 ) Personal communication to FlyBase  ( 10 ) FlyBase analysis  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 3 ) Book  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 2 )
	Cooper and Kennison, 2011, PLoS ONE 6(8): e23509 Molecular Genetic Analyses of Polytene Chromosome Region 72A-D in Drosophila melanogaster Reveal a Gene Desert in 72D. [FBrf0214748] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Show all research papers ...