DNApol-delta, DNApol-δ, DNA polymerase delta, DNA-polymerase-δ, Polδ
Please see the JBrowse view of Dmel\PolD1 for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.44
Gene model reviewed during 5.46
4 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
1092 (aa); 120 (kD observed); 125 (kD predicted)
Catalytic component of the DNA polymerase delta complex consisting of three subunits: the catalytic subunit PolD1 and two accessory subunits PolD2/Pol31 and PolD3/Pol32 (PubMed:31100062). Within the delta complex, interacts with both PolD2 and PolD3, and is able to interact with PolD2 in the absence of PolD3 (PubMed:31100062). Interacts with PCNA AND PCNA2 (PubMed:17087725).
The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\PolD1 using the Feature Mapper tool.
Comment: maternally deposited
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as ventral nerve cord anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
GBrowse - Visual display of RNA-Seq signals
View Dmel\PolD1 in GBrowse 2Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Source for identity of: PolD1 DNApol-δ
Source for merge of: DNApol-δ l(3)72Ac
The 'l(3)72Ac' (DNApol-δ) complementation group comprises 13 EMS-induced mutant alleles.
Cloning and characterisation of DNApol-δ reveals the amino acid sequence is very highly conserved.
Subunits of DNA polymerase α and δ are purified from embryos and characterised.
DNA polymerase δ has been isolated to near homogeneity from early embryos of D.melanogaster. The enzyme is composed of two polypeptides, which are 138kD and 47kD in size. The enzyme responds to mouse PCNA and has proofreading nuclease activity.
DNApol-δ has been purified from 0-2hr embryos, it consists of a 120kD polypeptide and, unlike mammalian Polδ, is unresponsive to proliferating cell nuclear antigen (PCNA).
Mono Q anion exchange chromatography used to resolve DNA polymerase activities in 0-2.5hr embryos. DNApol-δ and DNApol-α180 distinguished by copurification with DNAprimase (DNApol-α180) or 3'-5'exonuclease activity (DNApol-δ), immunoblot analysis with DNApol-α specific antibodies, sensitivity to aphidicolin (both DNApol-α180 and DNApol-δ sensitive) and BuPdGTP (DNApol-α180 more sensitive), and processivity measurements with and without PCNA (DNApol-δ has PCNA-inducible high processivity).