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Gene Dmel\Uba3

General Information
Symbol	Dmel\Uba3	Species	D. melanogaster
Name	Ubiquitin activating enzyme 3	Annotation symbol	CG13343
Feature type	protein_coding_gene	FlyBase ID	FBgn0263697
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	50C6-50C6	Sequence location	2R:9,746,462..9,747,937 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ubiquitin activating enzyme 3 is referred to in FlyBase by the symbol Dmel\Uba3 (CG13343, FBgn0263697). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: NEDD8 activating enzyme activity. There is experimental evidence that it is involved in the biological process: protein neddylation; negative regulation of smoothened signaling pathway. 4 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: E2 binding; Molybdenum cofactor biosynthesis, MoeB; NAD(P)-binding domain; UBA/THIF-type NAD/FAD binding fold; Ubiquitin activating enzyme, alpha domain; Ubiquitin-activating enzyme; Ubiquitin-activating enzyme repeat; Ubiquitin-activating enzyme, E1, active site. Gene sequence location is 2R:9746462..9747937.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	New/Merged/Split Record This record was either newly added to FlyBase for this release or is the result of merging or splitting existing records in this release
FB2012_01	Sequence features BKN22123 HFA06377
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 50C6-50C6   Limits computationally determined from genome sequence between P{lacW}l(2)03105k16702&P{lacW}fl(2)dk16105 and P{lacW}shotk03010
Experimentally Determined Cytological Location
Cytogenetic map Notes References 50C6-50C6     (Oh et al., 2003)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Uba3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Uba3-RA FBtr0087585  NM_137069   1476   450
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Uba3-PA   FBpp0086711   50.0   450   5.08     NP_610913   AAF58323
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ubiquitin activating enzyme, alpha domain (IPR023318) Ubiquitin-activating enzyme repeat (IPR000127) UBA/THIF-type NAD/FAD binding fold (IPR000594) Molybdenum cofactor biosynthesis, MoeB (IPR009036) Ubiquitin-activating enzyme, E1, active site (IPR018074) E2 binding (IPR014929) NAD(P)-binding domain (IPR016040) Ubiquitin-activating enzyme (IPR019572)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA694888   AA941449   AE013599 AAF58323   AB082729   AI515576   AW943904   AY052016 AAK93440   BF486203   BG636209   BG640784   BI354502   BI357812   BI565335   BI565968   BI566524   BI575606   BI575645   BI585406   BI604643   BI606142   BI607957   BI634463   BP559077   BT023713   CK604343   CK604344   EV586745   GH739010   GH739959   GH740488   GH740947   GH742375   GH742874   GH744281   GH746138   GH746344   GH747338   GH762314   GH762741   GH762991   GH765317   GH768152   GH769722
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9V6U8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. [an error occurred while processing this directive] modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage | heat sensitive, with Scer\GAL4Bx-MS1096 Uba3GD7988 Uba3KK102057 lethal | recessive Uba3SH2028 semi-lethal | heat sensitive, with Scer\GAL4Bx-MS1096 Uba3GD7988 Uba3KK102057 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 Uba3GD7988 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 Uba3GD7988 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 Uba3GD7988 wing blade | heat sensitive, with Scer\GAL4Bx-MS1096 Uba3GD7988 Uba3KK102057
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of Uba3 Class Mutagen Stocks Known lesion Uba3SH2028 amorphic allele - molecular evidence P-element activity 1 Yes Uba3G8197 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Uba3 Class Mutagen Stocks Known lesion Uba3GD7988 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Uba3KK102057 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel7128 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Uba3SH2028 No insertion of mobile activating element P{EP}Uba3G8197 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
	There is no information supporting a cellular component term for this gene. (Based on a review of available data on: 2006-10-26)
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
NEDD8 activating enzyme activity inferred from direct assay (Du et al., 2011)
Biological Process
CV term Evidence References
negative regulation of smoothened signaling pathway inferred from mutant phenotype (Du et al., 2011) protein neddylation inferred from direct assay (Du et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000127, InterPro:IPR014929 (FlyBase Curators et al., 2004-) NEDD8 ligase activity inferred from sequence or structural similarity with UniProtKB:O88598 (FlyBase, 1992-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Uba3 allele Gene References
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs	Dana\GF13717 Dere\GG20409 Dgri\GH21039 Dmoj\GI18642 Dper\GL17480 Dpse\GA12220 Dsec\GM21498 Dsim\GD10991 Dvir\GJ21656 Dwil\GK21978 Dyak\GE12570
Curated drosophilid orthologs	Dpse\GA12220
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	27977 y1 w*; P{EP}Uba3G8197/CyO
Kyoto	122114 w*; P{lacW}Uba3SH2028
VDRC	v105141 P{KK102057}VIE-260B v17137 w1118 P{GD7988}v17137 v17139 w1118; P{GD7988}v17139
Genomic Clones ( 2 )
	BACR06M19 BACR16G12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 38 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH24506 LD47462
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT20428 GM01733 GM27050 LD25592 RE44393 RH12065 RH24967 RH31342 RH31404 RH35021 RH36092 RH38626 RH63087 RH70075 RH72345 SD11931 SD13820
Other clones	Dmel_neb_003912_0136_3598 FGM318G06 RP001001049 RP001003824 RP001004154 RP001007321 RP001007477 RP001018078 RP001019848 RP001020658 RP001030321 RP001032814 RP001041702 RP001043773 RP001046930 RP001048027 RP001052649 RP001054412 UT01112
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Uba3 CG13343 (Du et al., 2011)
Source for database merge of	Source for merge of: CG13343 l(2)SH2028 (Du et al., 2011)
Additional comments
Other Comments
	1 allele of l(2)SH2028 recovered in a P-insertion screen. (Oh et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_137069 RefSeq (Proteins) • NP_610913 DDBJ / EMBL / GenBank • AAF58323 AAK93440 AB082729 AI515576 AW943904 AY052016 Entrez Gene - A searchable database of RefSeq genes. • 36539 UniProtKB/TrEMBL • Q9V6U8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ubiquitin activating enzyme, alpha domain (IPR023318) Ubiquitin-activating enzyme repeat (IPR000127) UBA/THIF-type NAD/FAD binding fold (IPR000594) Molybdenum cofactor biosynthesis, MoeB (IPR009036) Ubiquitin-activating enzyme, E1, active site (IPR018074) E2 binding (IPR014929) NAD(P)-binding domain (IPR016040) Ubiquitin-activating enzyme (IPR019572)
Linkouts
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG13343 (Du et al., 2011, Keleman et al., 2009.8.5) dUba3 (Du et al., 2011) l(2)SH2 2028 (Oh et al., 2002.3.26) l(2)SH2028   Uba3
Name Synonym	lethal (2) SH2028   Ubiquitin activating enzyme 3
Secondary FlyBase IDs
	FBgn0033882 FBgn0065282
References ( 15 )
Research paper	Du et al., 2011, PLoS ONE 6(9): e24168 In Vivo RNAi Screen Reveals Neddylation Genes as Novel Regulators of Hedgehog Signaling. [FBrf0215574]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452] Oh et al., 2003, Genetics 163(1): Table S1. [FBrf0157097]
Personal communication to FlyBase	Bellen, 2009.6.29, BDGP_2009_0629.GenExel.xml BDGP_2009_0629.GenExel.xml [FBrf0208121] Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335]
FlyBase analysis	FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
DNA/RNA sequence record	Oh et al., 2002.3.26, GenBank/EMBL/DDBJ: AB082729 Drosophila melanogaster DNA, clone:l(2)SH2 2028, genomic survey sequence. [FBrf0196548]
Computer file	BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]
Automatic genome annotation	FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126705]
Curated genome annotation	Wang, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126695]