FlyBase Gene Report: Dmel\Myo10A

General Information

Symbol

Dmel\Myo10A

Species

D. melanogaster

Name

Myosin 10A

Annotation Symbol

CG43657

Feature Type

protein_coding_gene

FlyBase ID

FBgn0263705

Gene Model Status

Current

Stock Availability

19 publicly available

Enzyme Name (EC)

myosin ATPase (5.6.1.8)

Gene Summary

Myosin 10A (Myo10A) encodes an unconventional myosin of the MyTH-FERM superclass that traffics sensory, cytoskeletal, and adhesion cargos. It is required during dorsal closure for proper epithelial alignment and for zippering and fusion of the two epithelial sheets. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Myosin XV

Key Links

Genomic Location

Cytogenetic map

9F12-9F13

Sequence location

X:10,930,840..10,964,046 [+]

Recombination map

1-33

RefSeq locus

NC_004354 REGION:10930840..10964046

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:Hsp26; FB:FBgn0001225

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:crp; FB:FBgn0001994

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:shg; FB:FBgn0003391

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:αTub84D; FB:FBgn0003885

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:RpL23; FB:FBgn0010078

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:smg; FB:FBgn0016070

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:Cpsf160; FB:FBgn0024698

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:inc; FB:FBgn0025394

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:Bap60; FB:FBgn0025463

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:Eb1; FB:FBgn0027066

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:Kap-α3; FB:FBgn0027338

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:CG2258; FB:FBgn0029997

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:ENGase; FB:FBgn0030839

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:Socs16D; FB:FBgn0030869

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:CG2765; FB:FBgn0035087

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:CG18135; FB:FBgn0036837

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:Fip1; FB:FBgn0037255

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:CG6834; FB:FBgn0037935

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:RpL34a; FB:FBgn0039406

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:Kat60; FB:FBgn0040208

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:Nedd4; FB:FBgn0259174

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:aPKC; FB:FBgn0261854

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:milt; FB:FBgn0262872

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:tgo; FB:FBgn0264075

(Liu et al., 2008)

inferred from physical interaction with FLYBASE:elgi; FB:FBgn0283649

(Liu et al., 2008)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables actin filament binding

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

enables ATP binding

inferred from electronic annotation with InterPro:IPR001609

(InterPro Project Members, 2004-)

enables cytoskeletal motor activity

inferred from electronic annotation with InterPro:IPR001609

(InterPro Project Members, 2004-)

enables microfilament motor activity

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in dorsal closure

inferred from mutant phenotype

(Liu et al., 2008)

involved_in filopodium assembly

inferred from mutant phenotype

(Liu et al., 2008)

involved_in intracellular protein transport

inferred from mutant phenotype

(Liu et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in actin filament organization

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

Cellular Component (8 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in filopodium

inferred from direct assay

(Liu et al., 2008)

located_in neuronal cell body

inferred from direct assay

(Liu et al., 2008)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

is_active_in actin cytoskeleton

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

is_active_in actin-based cell projection

inferred from biological aspect of ancestor with PANTHER:PTN002662420

(GO Reference Genome Project, 2011-)

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

located_in cytoskeleton

inferred from electronic annotation with InterPro:IPR000299, InterPro:IPR000857

(InterPro Project Members, 2004-)

is_active_in membrane

inferred from biological aspect of ancestor with PANTHER:PTN000320364

(GO Reference Genome Project, 2011-)

part_of myosin complex

inferred from electronic annotation with InterPro:IPR001609

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

MYOSIN MOTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

microfilament motor activity

ATP + H2O + myosin bound to actin filament at position(n) = ADP + phosphate + myosin bound to actin filament at position(n+1) (5.6.1.8)

Summaries

Gene Snapshot

Gene Group (FlyBase)

MYOSIN MOTORS -: Myosin motors are motor proteins that use the hydrolysis of ATP to drive movement along actin filaments. They can be divided into two groups: conventional myosins (myosin II) are involved in generating the mechanical force for muscle contraction; unconventional myosins (all other classes) are involved in processes such as cell motility, cytokinesis and trafficking. (Adapted from FBrf0134714.)

Summary (Interactive Fly)

cytoskeletal motor protein required during dorsal closure for the correct alignment of cells on opposing sides of the fusing epithelial sheets and for adhesion of the cells during the final zippering/fusion phase

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Myo10A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q0KHU0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.52

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.42

merge of CG16922 and Myo10A

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Myo10A-RC

FBtr0310333

NM_132441

10362

3054

Myo10A-RD

FBtr0310334

NM_001042804

10635

3145

Myo10A-RE

FBtr0310332

NM_001272506

5244

1069

Myo10A-RF

FBtr0346278

NM_001298174

10459

3054

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Myo10A-PC

FBpp0302016

339.2

3054

8.19

FBpp0302017

349.4

3145

8.47

FBpp0302015

119.7

1069

6.41

FBpp0312035

339.2

3054

8.19

Q9VZ45

NP_001285103

AHN59573

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

3054 aa isoforms: Myo10A-PC, Myo10A-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Myo10A using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.79

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in filopodium

inferred from direct assay

(Liu et al., 2008)

located_in neuronal cell body

inferred from direct assay

(Liu et al., 2008)

Expression Deduced from Reporters

Reporter: PBac{802.P.SVS-2}Myo10A^CPTI003907

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

Reporter: PBac{803.P.SVS-0}Myo10A^CPTI003738

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain cell body rind

Comment: punctate expression in adult brain cortex

(Knowles-Barley, 2011.8.24)

lamina

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Myo10A in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Myo10A

View exonic expression by tissue for Dmel\Myo10A

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263705

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32028/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263705

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263705

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2174

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Myo10A

Transgenic constructs containing regulatory region of Myo10A

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 10 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Myo10A^f03968

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Sterility

Allele

fertile

Myo10A^f03968

Other Phenotypes

Allele

abnormal flight, with Scer\GAL4^Mef2.PR

Myo10A^GD9734

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (46)

Hsap\MYO15A

12 of 14

Yes

Hsap\MYO15B

5 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\MYO9B

2 of 14

Yes

Hsap\MYO19

2 of 14

Hsap\PLEKHH3

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (38)

Rnor\Myo15a

12 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Rnor\Myo15b

3 of 14

Yes

Rnor\Myh3

2 of 14

Rnor\Myo9a

2 of 14

Yes

Rnor\Myo9b

2 of 14

Yes

Rnor\Myo16

2 of 14

Rnor\Plekhh3

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (42)

Mmus\Myo15

12 of 14

Yes

Mmus\Myo15b

7 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Mmus\Myo3a

2 of 14

Mmus\Myo3b

2 of 14

Mmus\Myo9a

2 of 14

Yes

Mmus\Myo9b

2 of 14

Yes

Mmus\Myo19

2 of 14

Mmus\Plekhh3

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (31)

Xtro\myo15b

4 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\otud6b

1 of 13

Xtro\rpl11

1 of 13

Xtro\st6galnac4

1 of 13

Danio rerio (Zebrafish) (61)

Drer\myo15ab

12 of 14

Yes

Drer\myo15aa

10 of 14

Yes

Drer\myo15b

5 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Drer\myo10l1

3 of 14

Yes

Drer\si:ch211-18i17.2

3 of 14

Yes

Drer\LOC556989

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\myo9ab

2 of 14

Yes

Drer\myo9b

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (22)

Cele\hum-4

5 of 14

Yes

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (16)

Agam\AgaP_AGAP005213

11 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (28)

Atha\ATM1

3 of 13

Yes

Atha\ATM2

3 of 13

Yes

Atha\MYA1

3 of 13

Yes

Atha\MYA2

3 of 13

Yes

Atha\VIIIA

3 of 13

Yes

Atha\VIIIB

3 of 13

Yes

Atha\XIA

3 of 13

Yes

Atha\XIB

3 of 13

Yes

Atha\XIC

3 of 13

Yes

Atha\XID

3 of 13

Yes

Atha\XIE

3 of 13

Yes

Atha\XIF

3 of 13

Yes

Atha\XIG

3 of 13

Yes

Atha\XIH

3 of 13

Yes

Atha\XI-I

3 of 13

Yes

Atha\XIJ

3 of 13

Yes

Atha\XIK

3 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\MYO2

3 of 13

Yes

Scer\MYO4

3 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (8)

Spom\myo51

3 of 12

Yes

Spom\myo52

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Myo10A. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (16)

6 of 13

didum

6 of 13

Myo28B1

6 of 13

jar

4 of 13

dachs

3 of 13

Mhc

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

zip

3 of 13

Mhcl

2 of 13

ninaC

2 of 13

Prm

2 of 13

CG31638

1 of 13

CG43867

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

MYO15A; myosin XVA

model of autosomal recessive nonsyndromic deafness 3

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MYO15A; myosin XVA

12 of 14

602666

DEAFNESS, AUTOSOMAL RECESSIVE 3; DFNB3

autosomal recessive nonsyndromic deafness 3

5 of 14

3 of 14

GRISCELLI SYNDROME, TYPE 1; GS1

Griscelli syndrome type 1

MYO5B; myosin VB

3 of 14

606540

microvillus inclusion disease

3 of 14

3 of 14

3 of 14

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Myo10A - Bap60

two hybrid

(Liu et al., 2008)

Myo10A - CG18135

two hybrid, pull down, autoradiography

(Liu et al., 2008)

Myo10A - CG2258

pull down, autoradiography, two hybrid

(Liu et al., 2008)

Myo10A - CG2765

two hybrid

(Liu et al., 2008)

Myo10A - CG6834

pull down, autoradiography, two hybrid

(Liu et al., 2008)

Myo10A - Cpsf160

two hybrid, pull down, autoradiography

(Liu et al., 2008)

Myo10A - ENGase

two hybrid

(Liu et al., 2008)

Myo10A - Eb1

two hybrid, pull down, autoradiography

(Liu et al., 2008)

Myo10A - Fip1

two hybrid, pull down, autoradiography

(Liu et al., 2008)

Myo10A - Hsp26

pull down, autoradiography, two hybrid

(Liu et al., 2008)

Myo10A - Kap-α3

two hybrid, pull down, autoradiography

(Liu et al., 2008)

Myo10A - Kat60

pull down, autoradiography, two hybrid

(Liu et al., 2008)

Myo10A - Nedd4

pull down, autoradiography, two hybrid

(Liu et al., 2008)

(Liu et al., 2008)

(Liu et al., 2008)

pull down, autoradiography, two hybrid

(Liu et al., 2008)

Myo10A - aPKC

two hybrid, pull down, autoradiography

(Liu et al., 2008)

Myo10A - crp

two hybrid, pull down, autoradiography

(Liu et al., 2008)

Myo10A - elgi

pull down, autoradiography, two hybrid

(Liu et al., 2008)

Myo10A - inc

two hybrid

(Liu et al., 2008)

Myo10A - milt

two hybrid, pull down, autoradiography

(Liu et al., 2008)

Myo10A - shg

anti bait coimmunoprecipitation, western blot, two hybrid, pull down, autoradiography

(Liu et al., 2008)

Myo10A - smg

pull down, autoradiography, two hybrid

(Liu et al., 2008)

Myo10A - tgo

two hybrid

(Liu et al., 2008)

Myo10A - αTub84D

pull down, autoradiography, two hybrid

(Liu et al., 2008)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0263705

MIST (protein-protein) - An integrated Molecular Interaction Database

32028

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-33

Cytogenetic map

9F12-9F13

Sequence location

X:10,930,840..10,964,046 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

9F12-9F13

Limits computationally determined from genome sequence between P{EP}EP1321^EP1321&P{EP}CG2061^EP1537 and P{EP}CG11756^EP1610

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Cytogenetic location of 10A1--10A2 based on known data for C901, v and sev, previous data described in FBrf0073632, and comparison with the physical map (Release 5.8).

(Babenko et al., 2009)

10A1-10A2

(Babenko et al., 2009)

10A1-10A1

(Tzolovsky, 2001.11.30)

Experimentally Determined Recombination Data

Location

1-33

(FlyBase, 2016)

1-34.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (19)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

24780

P{UASp-Myo10A.GFP}10, w^*

24781

w^*; P{UASp-Myo10A.GFP}20

24782

w^*; P{UASp-Myo10A.GFP}30

24783

P{UASp-Myo10A.RFP}11, w^*

24784

w^*; P{UASp-Myo10A.RFP}22

24785

w^*; P{UASp-Myo10A.RFP}33

11433

2136R-1

2136R-2

16922R-1

v101729

v33486

w¹¹¹⁸; P{GD9734}v33486

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (17)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP09748

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Embryos injected with dsRNA made from templates generated with primers directed against this gene exhibit misaligned stripes and delayed or incomplete dorsal hole closure. 68% of embryos show abnormal constriction of the leading edge cells in opposing segments; these embryos progress slowly and fail to close. The leading edge cells are missing filopodia in embryos of this phenotype. Embryos injected with Myo10A dsRNA that show proper epithelial matching exhibit an intermediate phenotype and have lamellipodia-like extensions instead of filopodia at their leading edge. Additionally, embryos injected with Myo10A dsRNA show disruptions to both actin and microtubule cytoskeletal organization, with disruptions being particularly severe near the leading edge.

(Liu et al., 2008)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: Myo10A CG2136

(FlyBase Genome Annotators, 2002-2003)

Source for merge of: Myo10A CG16922

(FlyBase Genome Annotators, 2011)

Additional comments

Annotations CG2174 and CG16922 merged as CG43657 in release 5.42 of the genome annotation.

(FlyBase Genome Annotators, 2011)

Merge supported by RNA-seq junction and RNA-seq expression data.

(FlyBase Genome Annotators, 2011)

Annotations CG2174 and CG2136 merged as CG2174 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for identity of Myo10A CG2174 was sequence comparison ( date:020316 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Myo10A CG2174

(FlyBase, 1996-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

10A

(Berg et al., 2001)

AAF47980

(Yamashita et al., 2000)

CG16922

(Japanese National Institute of Genetics, 2012.5.21, Babenko et al., 2009, Keleman et al., 2009.8.5, Hutter, 2007)

CG2136

CG2174

(Hosono et al., 2015, Petzoldt et al., 2012, Keleman et al., 2009.8.5, Liu et al., 2008, Rogers et al., 2003, Goldstein and Gunawardena, 2000)

CG43657

(FlyBase Genome Annotators, 2011, Ni et al., 2010.12.1)

DmXV

(Yamashita et al., 2000)

Myo10A

(Walls et al., 2020, Yoong et al., 2020, Jiang et al., 2019, Yee et al., 2019, Gene Disruption Project members, 2018-, Lebreton et al., 2018, David-Morrison et al., 2016, Vernes, 2014, Zhimulev et al., 2014, Japanese National Institute of Genetics, 2012.5.21, FlyBase Genome Annotators, 2011, Sánchez-Soriano et al., 2010, Wasbrough et al., 2010, Babenko et al., 2009, Parkhurst, 2009.3.19, Kwon et al., 2008, Hutter, 2007)

Myo10a

(Sutton et al., 2024)

Myo20

(Kollmar, 2015.9.15)

Syph

(Liu et al., 2008)

myo10A

(Petzoldt et al., 2012)

Name Synonyms

Myosin 10A

Myosin XV

(Rogers et al., 2003, Yamashita et al., 2000)

Sisyphus

(Liu et al., 2008)

myosin XV

(Rogers et al., 2003)

unconventional myosin class XV

Secondary FlyBase IDs

FBgn0030252
FBgn0030255
FBgn0030254

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32028

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q0KHU0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q0KHU0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0263705

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32028/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0263705

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0263705

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG43657

MARRVEL_MODEL - MARRVEL (model organism gene)

32028

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0263705

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0263705

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2174

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC006465

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

MIST (protein-protein) - An integrated Molecular Interaction Database

32028

References (75)

The following external sites may use different assemblies or annotations than FlyBase.

-0.79

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