columbus, clb, l(3)01152, HMGR, HMG-CoA reductase
catalyzes the biosynthesis of a mevalonate precursor for isoprenoids - functions in the hedgehog signaling pathway, implicated in the production of a signal by the somatic gonadal precursor cells that attracts migrating germ cells
Please see the JBrowse view of Dmel\Hmgcr for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.47
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 6.02
4.0, 3.2 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
916 (aa); 98 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hmgcr using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Hmgcr transcripts are expressed in a dynamic pattern in the embryo. They are expressed in the target tissue of the primordial germ cells (PGCs) which is the gonadal mesoderm. Expression in the mesoderm is initially broad and then is restricted to a segmental pattern. This occurs at the stage when the PGCs migrate from the endoderm to the mesoderm. Mesodermal expression of Hmgcr is limited to a cluster of cells in each of parasegments 10, 11, and 12 at the location of the gonadal mesoderm. The three clusters of Hmgcr-expressing cells join to form a continuous band, which is tightly associated with the PGCs. Expression is also observed in the dorsal vessel, in regions of the foregut, midgut, and hindgut, in clusters of cells along the ventral midline, and in the salivary gland.
The 4.0kb Hmgcr transcript is detected at all developmental stages with the highest levels in late embryos.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Hmgcr in JBrowsePlease Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Ortholog of B. mori juvenile-hormone-related gene (involved in JH biosynthesis, metabolism or signaling).
Isolated in an EMS mutagenesis screen to identify zygotic mutations affecting germ cell migration at discrete points during embryogenesis.
Mutants exhibit specific germ cell migration defects.
The Hmgcr gene product has a critical developmental function in providing spatial information to guide migrating primordial germ cells.
The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.
Encodes 3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-controlling enzyme for cholesterol synthesis in mammals. In D.melanogaster, the enzyme is not regulated by sterols (Brown et al., 1983; Silberkang et al., 1983); it synthesizes mevalonate for the production of nonsterol isoprenoids which are needed for growth and differentiation. The enzyme is found in cultured Kc and Schneider cells.
Source for merge of: Hmgcr l(3)01152
Source for merge of: Hmgcr BcDNA:LD15354
Source for merge of: Hmgcr l(3)04684
Source for merge of Hmgcr BcDNA:LD15354 was a shared cDNA ( date:030728 ).
