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Gene Dmel\Rcd5

General Information
Symbol	Dmel\Rcd5	Species	D. melanogaster
Name	Reduction in Cnn dots 5	Annotation symbol	CG1135
Feature type	protein_coding_gene	FlyBase ID	FBgn0263832
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	MCRS2, MCRS1, dMCRS2
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	64A4-64A5	Sequence location	3L:4,040,529..4,042,597 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Reduction in Cnn dots 5 is referred to in FlyBase by the symbol Dmel\Rcd5 (CG1135, FBgn0263832). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: mitotic G2 DNA damage checkpoint; centriole replication. 10 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Forkhead-associated (FHA) domain; Microspherule protein, N-terminal domain; SMAD/FHA domain. Gene sequence location is 3L:4040529..4042597.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 64A4-64A5   Limits computationally determined from genome sequence between P{PZ}Sc205634 and P{PZ}l(3)rG166rG166; Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 64A4-64A5   (determined by in situ hybridisation)   (Spradling et al., 1999) 64A4-64A5     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rcd5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.46 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rcd5-RA FBtr0073202  NM_139595   1947   578
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rcd5-PA   FBpp0073058   63.5   578   9.94     NP_647852   AAF47828
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Forkhead-associated (FHA) domain (IPR000253) SMAD/FHA domain (IPR008984) Microspherule protein, N-terminal domain (IPR025999)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201487   AA391381   AA438558   AA803611   AA942101   AA949056   AA950326   AA978486   AA978744   AA978964   AE014296 AAF47828   AI062563   AI293742   AI293871   AI386914   AI388040   AI513124   AI515595   AI517859   AI518273   AI518289   AI519396   AI519581   AI531691   AQ026355   AY060599 AAL28147   BI169088   BI212131   BI212609   BI229737   BI229877   BI234272   BI236146   BI371740   BI637660   BP550466   CO155096   CO177080   CO272285   CO277057   CO278445   CO290048   CO310143   CO311047   CO315105   CO317448   CO325515   CZ466205   CZ471190   EC061142   EC089539   EC195728   EC198298   EC214328   EC214333   EC227194   EC227527   EC232330   EC236680   EC242194   EC261979   EV594602   GH765190   GH779465
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VZJ3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Wagner et al., 2007, Dobbelaere et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    27 central nervous system, pupae P8    13 head, virgin 1-day female    9 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    12 head, mated 1-day male    13 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    10 fat body, larvae L3 wandering    9 fat body, white prepupae    13 fat body, pupae P8    5 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    13 ovary, virgin 4-day female    47 ovary, mated 4-day female    53 testis, mated 4-day male    28 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (46) central nervous system, larvae L3  (27) central nervous system, pupae P8    13 head, virgin 1-day female    9 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    12 head, mated 1-day male    13 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    10 fat body, larvae L3 wandering    9 fat body, white prepupae    13 fat body, pupae P8    5 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    13 ovary, virgin 4-day female  (47) ovary, mated 4-day female  (53) testis, mated 4-day male  (28) accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    27 central nervous system, pupae P8    13 head, virgin 1-day female    9 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    12 head, mated 1-day male    13 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    10 fat body, larvae L3 wandering    9 fat body, white prepupae    13 fat body, pupae P8    5 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    13 ovary, virgin 4-day female    47 ovary, mated 4-day female    53 testis, mated 4-day male    28 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    27 central nervous system, pupae P8    13 head, virgin 1-day female    9 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    12 head, mated 1-day male    13 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    10 fat body, larvae L3 wandering    9 fat body, white prepupae    13 fat body, pupae P8    5 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    13 ovary, virgin 4-day female    47 ovary, mated 4-day female    53 testis, mated 4-day male    28 accessory gland, mated 4-day male    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    27 central nervous system, pupae P8    13 head, virgin 1-day female    9 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    12 head, mated 1-day male    13 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    10 fat body, larvae L3 wandering    9 fat body, white prepupae    13 fat body, pupae P8    5 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    13 ovary, virgin 4-day female    47 ovary, mated 4-day female    53 testis, mated 4-day male    28 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (46) central nervous system, larvae L3  27 central nervous system, pupae P8    13 head, virgin 1-day female    9 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    12 head, mated 1-day male    13 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    10 fat body, larvae L3 wandering    9 fat body, white prepupae    13 fat body, pupae P8    5 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    13 ovary, virgin 4-day female  (47) ovary, mated 4-day female  (53) testis, mated 4-day male  28 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    27 central nervous system, pupae P8    13 head, virgin 1-day female    9 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    12 head, mated 1-day male    13 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    10 fat body, larvae L3 wandering    9 fat body, white prepupae    13 fat body, pupae P8    5 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    13 ovary, virgin 4-day female    47 ovary, mated 4-day female    53 testis, mated 4-day male    28 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    46 central nervous system, larvae L3    27 central nervous system, pupae P8    13 head, virgin 1-day female    9 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    10 head, mated 4-day female    9 head, mated 20-day female    12 head, mated 1-day male    13 head, mated 4-day male    13 head, mated 20-day male    15 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    15 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    10 fat body, larvae L3 wandering    9 fat body, white prepupae    13 fat body, pupae P8    5 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    13 ovary, virgin 4-day female    47 ovary, mated 4-day female    53 testis, mated 4-day male    28 accessory gland, mated 4-day male    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    90 embryo 02-04hr    111 embryo 04-06hr    72 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    54 embryo 12-14hr    45 embryo 14-16hr    48 embryo 16-18hr    41 embryo 18-20hr    38 embryo 20-22hr    43 embryo 22-24hr    45 larva L1    21 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    28 white prepupae new    27 white prepupae 12hr    31 white prepupae 24hr    42 pupae 2d postWPP    32 pupae 3d postWPP    19 pupae 4d postWPP    20 adult male 01day    23 adult male 05day    29 adult male 30day    28 adult female 01day    40 adult female 05day    50 adult female 30day    51 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (90) embryo 02-04hr  (111) embryo 04-06hr  (72) embryo 06-08hr  (76) embryo 08-10hr  (76) embryo 10-12hr  (54) embryo 12-14hr  (45) embryo 14-16hr  (48) embryo 16-18hr  (41) embryo 18-20hr  (38) embryo 20-22hr  (43) embryo 22-24hr  (45) larva L1    21 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6  (27) larva L3 puffstage 7-9  (28) white prepupae new  (27) white prepupae 12hr  (31) white prepupae 24hr  (42) pupae 2d postWPP  (32) pupae 3d postWPP    19 pupae 4d postWPP    20 adult male 01day    23 adult male 05day  (29) adult male 30day  (28) adult female 01day  (40) adult female 05day  (50) adult female 30day  (51) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    90 embryo 02-04hr  (111) embryo 04-06hr    72 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    54 embryo 12-14hr    45 embryo 14-16hr    48 embryo 16-18hr    41 embryo 18-20hr    38 embryo 20-22hr    43 embryo 22-24hr    45 larva L1    21 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    28 white prepupae new    27 white prepupae 12hr    31 white prepupae 24hr    42 pupae 2d postWPP    32 pupae 3d postWPP    19 pupae 4d postWPP    20 adult male 01day    23 adult male 05day    29 adult male 30day    28 adult female 01day    40 adult female 05day    50 adult female 30day    51 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    90 embryo 02-04hr    111 embryo 04-06hr    72 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    54 embryo 12-14hr    45 embryo 14-16hr    48 embryo 16-18hr    41 embryo 18-20hr    38 embryo 20-22hr    43 embryo 22-24hr    45 larva L1    21 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    28 white prepupae new    27 white prepupae 12hr    31 white prepupae 24hr    42 pupae 2d postWPP    32 pupae 3d postWPP    19 pupae 4d postWPP    20 adult male 01day    23 adult male 05day    29 adult male 30day    28 adult female 01day    40 adult female 05day    50 adult female 30day    51 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    90 embryo 02-04hr    111 embryo 04-06hr    72 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    54 embryo 12-14hr    45 embryo 14-16hr    48 embryo 16-18hr    41 embryo 18-20hr    38 embryo 20-22hr    43 embryo 22-24hr    45 larva L1    21 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    28 white prepupae new    27 white prepupae 12hr    31 white prepupae 24hr    42 pupae 2d postWPP    32 pupae 3d postWPP    19 pupae 4d postWPP    20 adult male 01day    23 adult male 05day    29 adult male 30day    28 adult female 01day    40 adult female 05day    50 adult female 30day    51 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (90) embryo 02-04hr  (111) embryo 04-06hr  (72) embryo 06-08hr  (76) embryo 08-10hr  (76) embryo 10-12hr  (54) embryo 12-14hr  (45) embryo 14-16hr  (48) embryo 16-18hr  (41) embryo 18-20hr  (38) embryo 20-22hr  (43) embryo 22-24hr  (45) larva L1    21 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6  27 larva L3 puffstage 7-9  28 white prepupae new  27 white prepupae 12hr  31 white prepupae 24hr  (42) pupae 2d postWPP  32 pupae 3d postWPP    19 pupae 4d postWPP    20 adult male 01day    23 adult male 05day  29 adult male 30day  28 adult female 01day  (40) adult female 05day  (50) adult female 30day  (51) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    90 embryo 02-04hr  111 embryo 04-06hr    72 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    54 embryo 12-14hr    45 embryo 14-16hr    48 embryo 16-18hr    41 embryo 18-20hr    38 embryo 20-22hr    43 embryo 22-24hr    45 larva L1    21 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    28 white prepupae new    27 white prepupae 12hr    31 white prepupae 24hr    42 pupae 2d postWPP    32 pupae 3d postWPP    19 pupae 4d postWPP    20 adult male 01day    23 adult male 05day    29 adult male 30day    28 adult female 01day    40 adult female 05day    50 adult female 30day    51 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    90 embryo 02-04hr    111 embryo 04-06hr    72 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    54 embryo 12-14hr    45 embryo 14-16hr    48 embryo 16-18hr    41 embryo 18-20hr    38 embryo 20-22hr    43 embryo 22-24hr    45 larva L1    21 larva L2    15 larva L3 12hr old    15 larva L3 puffstage 1-2    20 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    28 white prepupae new    27 white prepupae 12hr    31 white prepupae 24hr    42 pupae 2d postWPP    32 pupae 3d postWPP    19 pupae 4d postWPP    20 adult male 01day    23 adult male 05day    29 adult male 30day    28 adult female 01day    40 adult female 05day    50 adult female 30day    51 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    21 embryonic 1182-4H    40 embryonic GM2    28 embryonic Kc167    55 embryonic S1    55 embryonic S3    13 leg disc CME L1    34 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    18 wing disc ML-DmD32    42 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    22 ovary fGS/OSS    53 ovary OSC    38 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (34) Schneider line 2 Sg4    21 embryonic 1182-4H  (40) embryonic GM2  (28) embryonic Kc167  (55) embryonic S1  (55) embryonic S3    13 leg disc CME L1  (34) wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9  (58) wing disc ML-DmD16-c3  (30) wing disc ML-DmD21    18 wing disc ML-DmD32  (42) haltere disc ML-DmD17-c3  (28) eye-antennal disc ML-DmD11  (27) antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1  (39) CNS ML-DmBG2-c2  (33) tumorous blood cells mbn2    22 ovary fGS/OSS  (53) ovary OSC  (38) ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    21 embryonic 1182-4H    40 embryonic GM2    28 embryonic Kc167    55 embryonic S1    55 embryonic S3    13 leg disc CME L1    34 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    18 wing disc ML-DmD32    42 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    22 ovary fGS/OSS    53 ovary OSC    38 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    21 embryonic 1182-4H    40 embryonic GM2    28 embryonic Kc167    55 embryonic S1    55 embryonic S3    13 leg disc CME L1    34 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    18 wing disc ML-DmD32    42 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    22 ovary fGS/OSS    53 ovary OSC    38 ovary OSS    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    21 embryonic 1182-4H    40 embryonic GM2    28 embryonic Kc167    55 embryonic S1    55 embryonic S3    13 leg disc CME L1    34 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    18 wing disc ML-DmD32    42 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    22 ovary fGS/OSS    53 ovary OSC    38 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (34) Schneider line 2 Sg4    21 embryonic 1182-4H  (40) embryonic GM2  28 embryonic Kc167  (55) embryonic S1  (55) embryonic S3    13 leg disc CME L1  (34) wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9  (58) wing disc ML-DmD16-c3  30 wing disc ML-DmD21    18 wing disc ML-DmD32  (42) haltere disc ML-DmD17-c3  28 eye-antennal disc ML-DmD11  27 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1  (39) CNS ML-DmBG2-c2  (33) tumorous blood cells mbn2    22 ovary fGS/OSS  (53) ovary OSC  (38) ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    21 embryonic 1182-4H    40 embryonic GM2    28 embryonic Kc167    55 embryonic S1    55 embryonic S3    13 leg disc CME L1    34 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    18 wing disc ML-DmD32    42 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    22 ovary fGS/OSS    53 ovary OSC    38 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    21 embryonic 1182-4H    40 embryonic GM2    28 embryonic Kc167    55 embryonic S1    55 embryonic S3    13 leg disc CME L1    34 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    58 wing disc ML-DmD16-c3    30 wing disc ML-DmD21    18 wing disc ML-DmD32    42 haltere disc ML-DmD17-c3    28 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    39 CNS ML-DmBG2-c2    33 tumorous blood cells mbn2    22 ovary fGS/OSS    53 ovary OSC    38 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    11 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    46 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    17 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    42 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    11 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult  (46) Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    17 Ethanol 2.5% 3 hrs, larvae L3  (45) Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (38) Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult  (42) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    11 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    46 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    17 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    42 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    11 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    46 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    17 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    42 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    11 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    46 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    17 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    42 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    11 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult  (46) Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    17 Ethanol 2.5% 3 hrs, larvae L3  (45) Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (38) Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult  (42) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    11 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    46 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    17 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    42 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    11 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    24 Cadmium 100 mM 48 hrs, 4-day adult    46 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    17 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    10 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    38 Caffeine 25 mg/ml 48 hrs, 4-day adult    20 Paraquat 5 mM 48 hrs, 4-day adult    42 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • GH01794
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4pnr-MD237 Rcd5GD5123 lethal | recessive Rcd5rG166 lethal | third instar larval stage (with Rcd5c00114) Rcd5c07041 Rcd5rG166 lethal | third instar larval stage (with Rcd5c07041) Rcd5c00114 Rcd5rG166 lethal | third instar larval stage (with Rcd5rG166) Rcd5c00114 Rcd5c07041 Phenotype manifest in Allele
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of Rcd5 Class Mutagen Stocks Known lesion Rcd5rG166 P-element activity 1 -- Rcd5c00114 piggyBac transposase 0 -- Rcd5c07041 piggyBac transposase 0 -- Rcd5ΔGr64 FLPase 0 Yes
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rcd5 Class Mutagen Stocks Known lesion Rcd5GD5123 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rcd5HMS01439 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rcd5KK101521 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rcd5MtnA.N.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Rcd5Scer\UAS.cAa in vitro construct - regulatory fusion 0 -- Rcd5t15.3 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Exel6099 (Ryder, 2004.4.26) Df(3L)GN24 (Spradling et al., 1999, Spradling et al., 1995, BDGP Project Members, 1994-1999)
Partially disrupted in	Df(3L)ΔGr64 (Slone et al., 2007)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK101521} NA P{TRiP.HMS01439} NA P{UAS-Rcd5.A} NA characterization construct P{t15.3.R1} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}Rcd5rG166 No miscellaneous insertions PBac{PB}Rcd5c00114 NA PBac{PB}Rcd5c07041 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
centriole replication inferred from mutant phenotype (Dobbelaere et al., 2008) mitotic G2 DNA damage checkpoint inferred from genetic interaction with nbs (Kondo and Perrimon, 2011)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Wagner et al., 2007, Dobbelaere et al., 2008)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
nucleolus inferred from sequence or structural similarity with EMBL:AF015309 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Rcd5 - AP-2μ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG10306 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG1091 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG11505 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG17593 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG1785 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG18178 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG32344 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG40172 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG4611 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG4612 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG5726 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG6937 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG7518 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG7903 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - CG8478 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Chro anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Clbn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Dp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - FLASH anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Gale anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Ip259 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Jra anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - MBD-R2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Rcd5 - Map205 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Mtpα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Ns1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Optix anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Rab5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - RpS27A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Rrp4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Rs1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - SmG anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Srp54 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Syp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Upf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - alt anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - btz anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - cathD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - eIF4AIII anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - kay anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mRpL13 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mRpL16 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mRpL2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mRpL21 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mRpL42 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mRpL48 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mRpL51 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mRpS28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mTerf3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - mod anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - nito anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - pUf68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - pav anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - pit anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - qkr54B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - qkr58E-3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - shot anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - snama anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - snf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rcd5 - wds anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rcd5 allele Gene References
External Data
Linkouts	SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VZJ3
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6H9Z5G Dipteran inclusive ortholog search EOG6XWG6D Insect inclusive ortholog search EOG6TMPXK Arthropod inclusive ortholog search EOG6N5TCC Metazoa inclusive ortholog search EOG6RFZM5
Orthologs in Drosophila Species (EOG6H9Z5G)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Rcd5     Drosophila simulans   Dsim\GD13805 Y   Drosophila sechellia   Dsec\GM14616 Y   Drosophila erecta   Dere\GG15185 Y   Drosophila yakuba   Dyak\GE21404 Y   Drosophila ananassae   Dana\GF10092 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10939 Y   Drosophila persimilis   Dper\GL17030 Y   Drosophila willistoni   Dwil\GK17772 Y   Drosophila virilis   Dvir\GJ11739 Y   Drosophila mojavensis   Dmoj\GI13885 Y   Drosophila grimshawi   Dgri\GH14562 Y
Orthologs in non-Drosophila Dipterans (EOG6XWG6D)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL000594   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004182   Anopheles gambiae Malaria mosquito  Agam\AGAP005560   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ004521
Orthologs in non-Dipteran Insects (EOG6TMPXK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB51662   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG006612   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12536   Atta cephalotes Leafcutter ant  Acep\ACEP14538   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19585   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20985   Linepithema humile Argentine ant  Lhum\LH14288   Pogonomyrmex barbatus Red harvester ant  Pbar\PB20293   Solenopsis invicta Red fire ant  Sinv\SINV24496   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006335   Bombyx mori Silkmoth  Bmor\BGIBMGA002680   Pediculus humanus Human body louse  Phum\PHUM043250   Tribolium castaneum Red flour beetle  Tcas\TC003716
Orthologs in non-Insect Arthropods (EOG6N5TCC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\ND2   Ixodes scapularis Deer tick  Isca\ISCW002145
Orthologs in non-Arthropod Metazoa (EOG6RFZM5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\H28O16.2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018004gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021734gn   Danio rerio Zebrafish  Drer\mcrs1   Xenopus tropicalis Western clawed frog  Xtro\mcrs1   Gallus gallus Domestic chicken  Ggal\ENSGALG00000000495   Mus musculus House mouse  Mmus\Mcrs1   Rattus norvegicus Norway rat  Rnor\Mcrs1   Homo sapiens Human  Hsap\ENSG00000187778
Human Orthologs (1)
Gene OMIM HGNC Hsap\ENSG00000187778
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD13805 Drosophila sechellia Dsec\GM14616 Drosophila erecta Dere\GG15185 Drosophila yakuba Dyak\GE21404 Drosophila ananassae Dana\GF10092 Drosophila pseudoobscura pseudoobscura Dpse\GA10939 Drosophila persimilis Dper\GL17030 Drosophila willistoni Dwil\GK17772 Drosophila virilis Dvir\GJ11739 Drosophila mojavensis Dmoj\GI13885 Drosophila grimshawi Dgri\GH14562
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	12065 P{PZ}Rcd5rG166 ry506/TM3, Sb1 35026 y1 sc* v1; P{TRiP.HMS01439}attP2
VDRC	v108017 P{KK101521}VIE-260B v15613 w1118; P{GD5123}v15613
Genomic Clones ( 1 )
	BACR09C02 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 59 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH01794
Other clones	DPiM_GH01794
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH17302 GH18772 GM13639 LD04565 LD10264 LD12359 LD26139 LD27806 LD28849 LD30713 LD32786 LD33184 LD37742 LD37761 LD38983 LD39217 LD42966 LD47483 LP06945 LP07119 RE10065 RE17153 RE17726 RE27578 RE27751 RE29950 RE32158 RE58693 SD02927 SD19719
Other clones	8168 17767 20697 100540 119436 119524 163667 167390.1 176976 524424 CK01.64C.F5 CK01.93C.B8 Dmel_neb_008693_0701_1015 EK052418 EK072940 EK106540 EK122806 EK130063 EK196704 EK220601 EK223225 EK239120 EK268114 EN05353 FGM207C09 RP001024898 RP001038238
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
	G. Rubin.
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Rcd5 CG1135 (Dobbelaere et al., 2008)
Source for database merge of	Source for merge of: Rcd5 l(3)rG166 (Andersen et al., 2010)
Additional comments
Other Comments
	Rcd5 is involved in centriole duplication and is required for efficient recruitment of pericentriolar material. (Dobbelaere et al., 2008) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in decreased γ-tubulin staining of the centrosome and spindle and the formation of an aberrantly long spindle when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype is not observed without Cdc27 dsRNA. (Goshima et al., 2007) Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells. (Nybakken et al., 2005)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_139595 RefSeq (Proteins) • NP_647852 DDBJ / EMBL / GenBank • AAF47828 AAL28147 AI062563 AI513124 AQ026355 AY060599 CZ466205 CZ471190 Entrez Gene - A searchable database of RefSeq genes. • 38478 UniProtKB/TrEMBL • Q9VZJ3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Forkhead-associated (FHA) domain (IPR000253) SMAD/FHA domain (IPR008984) Microspherule protein, N-terminal domain (IPR025999) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH01794
Linkouts
	SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VZJ3
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	CG1135 (Larschan et al., 2012, Ni et al., 2011.7.19, Slone et al., 2007, Mendjan et al., 2006, Wagner et al., 2007, Nybakken et al., 2005, Goshima et al., 2007, Dobbelaere et al., 2008, Wagner et al., 2007, Keleman et al., 2009.8.5, Li et al., 2010, Raja et al., 2010) dMCRS1 (Wagner et al., 2007) dMCRS2 (Mendjan et al., 2006, Prestel et al., 2010) l(3)r0166 (Spradling et al., 1995) l(3)rG166   MCRS1 (White et al., 2011, Kondo and Perrimon, 2011, Wagner et al., 2007) MCRS2 (Andersen et al., 2010, Prestel et al., 2010, Raja et al., 2010, Lam et al., 2012) p78 (Chen and Thomas, 2002) Rcd5 (Dobbelaere et al., 2008, )
Name Synonym	CG1135   lethal (3) rG166   Reduction in Cnn Dots 5 (Dobbelaere et al., 2008) Reduction in Cnn dots 5
Secondary FlyBase IDs
	FBgn0011495 FBgn0035489
References ( 37 )
Generate a list of	All references relating to this gene ( 37 )
List References by type	Research paper  ( 16 ) Supplementary material  ( 6 ) Review  ( 1 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 4 )
	Lam et al., 2012, PLoS Genet. 8(6): e1002736 The NSL Complex Regulates Housekeeping Genes in Drosophila. [FBrf0218697] Larschan et al., 2012, PLoS Genet. 8(7): e1002830 Identification of Chromatin-Associated Regulators of MSL Complex Targeting in Drosophila Dosage Compensation. [FBrf0219020] Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] White et al., 2011, J. Cell Biol. 193(4): 677--694 Drosophila histone locus bodies form by hierarchical recruitment of components. [FBrf0213662] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...