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Gene Dmel\Rcd5

General Information
Symbol	Dmel\Rcd5	Species	D. melanogaster
Name	Reduction in Cnn dots 5	Annotation symbol	CG1135
Feature type	protein_coding_gene	FlyBase ID	FBgn0263832
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	MCRS2, MCRS1, dMCRS2
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	64A4-64A5	Sequence location	3L:4,040,534..4,042,597 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Reduction in Cnn dots 5 is referred to in FlyBase by the symbol Dmel\Rcd5 (CG1135, FBgn0263832). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: mitotic cell cycle G2/M transition DNA damage checkpoint; centriole replication. 10 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Forkhead-associated (FHA) domain; SMAD/FHA domain. Gene sequence location is 3L:4040534..4042597.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	New/Merged/Split Record This record was either newly added to FlyBase for this release or is the result of merging or splitting existing records in this release
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 64A4-64A5   Limits computationally determined from genome sequence between P{PZ}Sc205634 and P{PZ}l(3)rG166rG166; Left limit from in situ hybridisation (FBrf0067338) Right limit from in situ hybridisation (FBrf0067338)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 64A4-64A5   (determined by in situ hybridisation)   (Spradling et al., 1999) 64A4-64A5     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rcd5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rcd5-RA FBtr0073202  NM_139595   1942   578
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rcd5-PA   FBpp0073058   63.5   578   9.94     NP_647852   AAF47828
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Forkhead-associated (FHA) domain (IPR000253) SMAD/FHA domain (IPR008984)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201487   AA391381   AA438558   AA803611   AA942101   AA949056   AA950326   AA978486   AA978744   AA978964   AE014296 AAF47828   AI062563   AI293742   AI293871   AI386914   AI388040   AI513124   AI515595   AI517859   AI518273   AI518289   AI519396   AI519581   AI531691   AQ026355   AY060599 AAL28147   BI169088   BI212131   BI212609   BI229737   BI229877   BI234272   BI236146   BI371740   BI637660   BP550466   CO155096   CO177080   CO272285   CO277057   CO278445   CO290048   CO310143   CO311047   CO315105   CO317448   CO325515   CZ466205   CZ471190   EC061142   EC089539   EC195728   EC198298   EC214328   EC214333   EC227194   EC227527   EC232330   EC236680   EC242194   EC261979   EV594602   GH765190   GH779465
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VZJ3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. [an error occurred while processing this directive] modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4pnr-MD237 Rcd5GD5123 lethal | recessive Rcd5rG166 lethal | third instar larval stage (with Rcd5c00114) Rcd5c07041 Rcd5rG166 lethal | third instar larval stage (with Rcd5c07041) Rcd5c00114 Rcd5rG166 lethal | third instar larval stage (with Rcd5rG166) Rcd5c00114 Rcd5c07041 Phenotype manifest in Allele
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of Rcd5 Class Mutagen Stocks Known lesion Rcd5rG166 P-element activity 1 -- Rcd5c00114 piggyBac transposase 0 -- Rcd5c07041 piggyBac transposase 0 -- Rcd5ΔGr64 FLPase 0 Yes
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rcd5 Class Mutagen Stocks Known lesion Rcd5GD5123 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rcd5HMS01439 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rcd5KK101521 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rcd5MtnA.N.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Rcd5Scer\UAS.cAa in vitro construct - regulatory fusion 0 -- Rcd5t15.3 in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Exel6099 (Ryder, 2004.4.26) Df(3L)GN24 (Spradling et al., 1999, Spradling et al., 1995, BDGP Project Members, 1994-1999)
Partially disrupted in	Df(3L)ΔGr64 (Slone et al., 2007)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK101521} NA P{TRiP.HMS01439} NA P{UAS-Rcd5.A} NA characterization construct P{t15.3.R1} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}Rcd5rG166 No miscellaneous insertions PBac{PB}Rcd5c00114 NA PBac{PB}Rcd5c07041 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
centriole replication inferred from mutant phenotype (Dobbelaere et al., 2008) mitotic cell cycle G2/M transition DNA damage checkpoint inferred from genetic interaction with nbs (Kondo and Perrimon, 2011)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Wagner et al., 2007)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
nucleolus inferred from sequence or structural similarity with EMBL:AF015309 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rcd5 allele Gene References
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs	Dana\GF10092 Dere\GG15185 Dgri\GH14562 Dmoj\GI13885 Dper\GL17030 Dpse\GA10939 Dsec\GM14616 Dsim\GD13805 Dvir\GJ11739 Dwil\GK17772 Dyak\GE21404
Curated drosophilid orthologs	Dpse\GA10939
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	12065 P{PZ}Rcd5rG166 ry506/TM3, Sb1 35026 y1 sc* v1; P{TRiP.HMS01439}attP2
VDRC	v108017 P{KK101521}VIE-260B v15613 w1118; P{GD5123}v15613
Genomic Clones ( 1 )
	BACR09C02 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 59 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH01794
Other clones	DPiM_GH01794
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH17302 GH18772 GM13639 LD04565 LD10264 LD12359 LD26139 LD27806 LD28849 LD30713 LD32786 LD33184 LD37742 LD37761 LD38983 LD39217 LD42966 LD47483 LP06945 LP07119 RE10065 RE17153 RE17726 RE27578 RE27751 RE29950 RE32158 RE58693 SD02927 SD19719
Other clones	8168 17767 20697 100540 119436 119524 163667 167390.1 176976 524424 CK01.64C.F5 CK01.93C.B8 Dmel_neb_008693_0701_1015 EK052418 EK072940 EK106540 EK122806 EK130063 EK196704 EK220601 EK223225 EK239120 EK268114 EN05353 FGM207C09 RP001024898 RP001038238
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
	G. Rubin.
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Rcd5 CG1135 (Dobbelaere et al., 2008)
Source for database merge of	Source for merge of: Rcd5 l(3)rG166 (Andersen et al., 2010)
Additional comments
Other Comments
	Rcd5 is involved in centriole duplication and is required for efficient recruitment of pericentriolar material. (Dobbelaere et al., 2008) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in decreased γ-tubulin staining of the centrosome and spindle and the formation of an aberrantly long spindle when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype is not observed without Cdc27 dsRNA. (Goshima et al., 2007) Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells. (Nybakken et al., 2005)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_139595 RefSeq (Proteins) • NP_647852 DDBJ / EMBL / GenBank • AAF47828 AAL28147 AI062563 AI513124 AQ026355 AY060599 CZ466205 CZ471190 Entrez Gene - A searchable database of RefSeq genes. • 38478 UniProtKB/TrEMBL • Q9VZJ3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Forkhead-associated (FHA) domain (IPR000253) SMAD/FHA domain (IPR008984)
Linkouts
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	CG1135 (Ni et al., 2011.7.19, Slone et al., 2007, Mendjan et al., 2006, Wagner et al., 2007, Nybakken et al., 2005, Goshima et al., 2007, Dobbelaere et al., 2008, Wagner et al., 2007, Keleman et al., 2009.8.5, Li et al., 2010, Raja et al., 2010) dMCRS1 (Wagner et al., 2007) dMCRS2 (Mendjan et al., 2006, Prestel et al., 2010) l(3)r0166 (Spradling et al., 1995) l(3)rG166   MCRS1 (White et al., 2011, Kondo and Perrimon, 2011, Wagner et al., 2007) MCRS2 (Andersen et al., 2010, Prestel et al., 2010, Raja et al., 2010) p78 (Chen and Thomas, 2002) Rcd5 (Dobbelaere et al., 2008, )
Name Synonym	CG1135   lethal (3) rG166   Reduction in Cnn Dots 5 (Dobbelaere et al., 2008) Reduction in Cnn dots 5
Secondary FlyBase IDs
	FBgn0011495 FBgn0035489
References ( 31 )
Generate a list of	All references relating to this gene ( 31 )
List References by type	Research paper  ( 14 ) Supplementary material  ( 4 ) Review  ( 1 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) Computer file  ( 4 ) Automatic genome annotation  ( 1 )
Recent research papers ( 7 )
	Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] White et al., 2011, J. Cell Biol. 193(4): 677--694 Drosophila histone locus bodies form by hierarchical recruitment of components. [FBrf0213662] Andersen et al., 2010, Mol. Cell. Biol. 30(19): 4744--4755 Drosophila MCRS2 Associates with RNA Polymerase II Complexes To Regulate Transcription. [FBrf0211794] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Li et al., 2010, BMC Cell Biol. 11: 7 Nutritional control of gene expression in Drosophila larvae via TOR, Myc and a novel cis-regulatory element. [FBrf0210137] Prestel et al., 2010, Mol. Cell 38(6): 815--826 The Activation Potential of MOF Is Constrained for Dosage Compensation. [FBrf0211211] Raja et al., 2010, Mol. Cell 38(6): 827--841 The Nonspecific Lethal Complex Is a Transcriptional Regulator in Drosophila. [FBrf0211222] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...