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General Information
Symbol
Dmel\BubR1
Species
D. melanogaster
Name
Bub1-related kinase
Annotation Symbol
CG7838
Feature Type
FlyBase ID
FBgn0263855
Gene Model Status
Stock Availability
Gene Snapshot
Bub1-related kinase (BubR1) encodes a motif-rich protein with a C-terminal serine-threonine kinase domain. During prometaphase and metaphase, it plays important roles in spindle assembly checkpoint signalling, stabilization of kinetochore-microtuble attachments and chromosome alignment. It is also essential to maintain sister-chromatid cohesion during meiotic progression and for normal maintenance of Synaptonemal Complex in females. [Date last reviewed: 2019-03-07]
Also Known As

bub1, l(2)k03113

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:5,969,099..5,974,385 [-]
Recombination map

2-55

RefSeq locus
NT_033778 REGION:5969099..5974385
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
Summaries
Gene Group (FlyBase)
OTHER CONVENTIONAL PROTEIN KINASE DOMAIN KINASES -
The group of 'other conventional protein kinase domain kinases' consists of kinases with a conventional eukaryotic protein kinase domain that do not fit into any of the other major groups. This grouping is described in FBrf0201870.
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\BubR1 or the JBrowse view of Dmel\BubR1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0086032
4626
1460
Additional Transcript Data and Comments
Reported size (kB)

4.601 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0085368
165.1
1460
6.27
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

1461 (aa); 165 (kD observed); 165 (kD predicted)

Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\BubR1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (15 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000361607
(assigned by GO_Central )
Biological Process (9 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000361607
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000361607
(assigned by GO_Central )
traceable author statement
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271
(assigned by InterPro )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000361607
(assigned by GO_Central )
traceable author statement
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
Additional Descriptive Data

Bub1 protein is detected at the kinetochore in chromosomes from colchicine treated S2 cells and larval neuroblasts. Bub1 protein shows cell-cycle dependent localization. Diffuse nucleoplasmic staining is detected at interphase. Strong kinetochore localization is found in prophase. This staining is weaker at prometaphase. At metaphase, no Bub1 protein is detected at chromosomes at the metaphase plate. Weak kintetochore staining may be seen in early anaphase, but not in late anaphase or telophase. Localization of Bub1 protein shows a similar dynamic distribution pattern in the first and second meiotic divisions during spermatogenesis.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\BubR1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 9 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 18 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of BubR1
Transgenic constructs containing regulatory region of BubR1
Deletions and Duplications ( 30 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
mitosis & nuclear chromosome
mitotic anaphase & nuclear chromosome
onion stage spermatid & nucleus
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
No
6 of 15
No
Yes
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
6 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
7 of 13
Yes
No
4 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
6 of 12
Yes
Yes
3 of 12
No
Yes
Danio rerio (Zebrafish) (3)
8 of 15
Yes
Yes
4 of 15
No
Yes
3 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
5 of 15
Yes
No
2 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (3)
4 of 9
Yes
No
1 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
8 of 15
Yes
Yes
6 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
5 of 12
Yes
Yes
3 of 12
No
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091901AJ )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501KH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01D2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Rhodnius prolixus
Kissing bug
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (1)
6 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 3 )
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
DroID - A comprehensive database of gene and protein interactions.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map

2-55

Cytogenetic map
Sequence location
2R:5,969,099..5,974,385 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
42A5-42A6
Limits computationally determined from genome sequence between P{PZ}l(2)0985109851&P{lacW}Src42Ak10108 and P{lacW}l(2)k09848k09848&P{EP}EP407
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
42A1-42A2
(determined by in situ hybridisation)
42A1-42A3
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (17)
Genomic Clones (13)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (43)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: BubR1 CG7838

Source for database merge of

Source for merge of: BubR1 mcl(2)Z1525

Additional comments

Previous molecular and genetic analysis (FBrf0109303) identified the CG7838 annotation as having significant homology to "Bub1" proteins from other organisms (FlyBase curator comment: this resulted in the "CG7838" gene being renamed to "Bub1" in FlyBase). However, the completion of the Drosophila genome revealed the existence of a second annotation, CG14030, that is also closely related to "Bub1" proteins. Higher eukaryotes also have two genes encoding proteins closely related to "Bub1", one of which retains the symbol "Bub1", while the other is called "BubR1". From phylogenetic analysis including both CG7838 and CG14030 it is difficult to determine unequivocally which gene codes for "Bub1" and which gene codes for "BubR1". However, functional analysis of these proteins in FBrf0174806 suggests that CG14030 encodes the homolog of Bub1 (FlyBase curator comment: as a consequence of this, the gene corresponding to the CG7838 annotation has been renamed "BubR1" and the gene corresponding to the CG14030 annotation has been renamed "Bub1" in FlyBase).

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

dsRNA against this gene has been used to treat SL2 cells to study the effect of depletion of BubR1 on the spindle checkpoint.

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, aneuploidy, an increase in mitotic index, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells, a whole range of mitotic abnormalities, central spindle defects, chromosome abnormalities, and lagging chromatids are seen.

BubR1 has a role in blocking exit from mitosis.

Loss of function mutations in BubR1 cause severe mitotic abnormalities consistent with accelerated transit through metaphase.

Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 40 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
KEGG Genes - Molecular building blocks of life in the genomic space.
SignaLink - A signaling pathway resource with multi-layered regulatory networks.
Linkouts
DroID - A comprehensive database of gene and protein interactions.
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (20)
Reported As
Symbol Synonym
BcDNA:LD23835
BubR1
(Huang et al., 2019, Levis, 2018.8.30, Resende et al., 2018, Tsui et al., 2018, Derive et al., 2015, Liang et al., 2014, Rowshanravan et al., 2014, Rubin et al., 2014, Sopko et al., 2014, Wong et al., 2014, Cane et al., 2013, Cipressa and Cenci, 2013, Conde et al., 2013, Feijão et al., 2013, Morais da Silva et al., 2013, Park et al., 2013, Schertel et al., 2013, Althoff et al., 2012, Delgehyr et al., 2012, Di Stefano et al., 2011, Neumüller et al., 2011, Przewloka et al., 2011, Shieh and Bonini, 2011, Toku et al., 2011, Venkei et al., 2011, Li et al., 2010, Nishiyama et al., 2010, Oliveira et al., 2010, Podhraski et al., 2010, Royou et al., 2010, Schittenhelm et al., 2010, Torras-Llort et al., 2010, Bucciarelli et al., 2009, Graham et al., 2009, Lince-Faria et al., 2009, Martins et al., 2009, Matos et al., 2009, Rahmani et al., 2009, Schittenhelm et al., 2009, Kwon et al., 2008, Pauli et al., 2008, Wen et al., 2008, Buffin et al., 2007, Garcia et al., 2007, Gilliland et al., 2007, Lehner et al., 2007, Li et al., 2007, Maia et al., 2007, Orr et al., 2007, Pandey et al., 2007, Blower et al., 2006, Cesario et al., 2006, Delcros et al., 2006, Laycock et al., 2006, Muller et al., 2006, Buffin, 2005, Buffin et al., 2005, Buffin et al., 2005, Dzhindzhev et al., 2005, Goshima and Vale, 2005, Heeger et al., 2005, Karess, 2005, Maia and Sunkel, 2005, Malmanche and Sunkel, 2005, Page et al., 2005, Perez-Mongiovi et al., 2005, Royou et al., 2005, Royou et al., 2005, Abad et al., 2004, Bettencourt-Dias et al., 2004, Fischer et al., 2004, Fisk and Winey, 2004, Logarinho et al., 2004, Parks, 2004.5.12, Parry et al., 2003, Maiato et al., 2002)
CG 7838
l(2)k06109
mcl(2)Z1525
Name Synonyms
Bub1-related kinase
bub related one
Secondary FlyBase IDs
  • FBgn0025458
  • FBgn0022226
  • FBgn0069180
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (183)