FB2021_02, released April 13, 2021

Gene: Dmel\ebi

Open Close
General Information
Symbol
Dmel\ebi
Species
D. melanogaster
Name
ebi
Annotation Symbol
CG4063
Feature Type
protein_coding_gene
FlyBase ID
FBgn0263933
Gene Model Status
Current
Stock Availability
24 publicly available
Gene Summary
ebi (ebi) encodes a nuclear-localized protein that regulates Egfr, Notch and Wg signaling pathways. ebi encoded product contributes to multiple processes including wing growth and eye development as well as regulation of transcription and innate immune response. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
21C2-21C2
Sequence location
2L:415,067..418,536 [+]
Recombination map
2-0.7
RefSeq locus
NT_033779 REGION:415067..418536
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (27 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
enables chromatin binding
inferred from direct assay
(Tsuda et al., 2006)
enables protein binding
inferred from physical interaction with FLYBASE:inferred from high throughput direct assayC3; FB:FBgn0025825
(Qi et al., 2008)
inferred from physical interaction with UniProtKB:Q27934
(assigned by UniProt )
(Boulton et al., 2000)
inferred from physical interaction with FLYBASE:Smyd4-2; FB:FBgn0036282
(Thompson and Travers, 2008)
enables repressing transcription factor binding
inferred from physical interaction with FLYBASE:sna; FB:FBgn0003448
(Qi et al., 2008)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables transcription corepressor activity
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (19 terms)
Terms Based on Experimental Evidence (17 terms)
CV Term
Evidence
References
involved_in cellular response to light stimulus
inferred from mutant phenotype
(Lim et al., 2012)
involved_in chaeta development
inferred from mutant phenotype
(Marygold et al., 2011)
involved_in epidermal growth factor receptor signaling pathway
inferred from genetic interaction with FLYBASE:Egfr; FB:FBgn0003731
(Dong et al., 1999)
involved_in imaginal disc-derived wing margin morphogenesis
inferred from mutant phenotype
(Nguyen et al., 2016)
involved_in negative regulation of JNK cascade
inferred from genetic interaction with FLYBASE:Jra; FB:FBgn0001291
(Lim et al., 2012)
inferred from genetic interaction with FLYBASE:hep; FB:FBgn0010303
(Lim et al., 2012)
inferred from genetic interaction with FLYBASE:egr; FB:FBgn0033483
(Lim et al., 2012)
inferred from genetic interaction with FLYBASE:puc; FB:FBgn0243512
(Lim et al., 2012)
involved_in negative regulation of neuron apoptotic process
inferred from mutant phenotype
(Lim et al., 2012)
involved_in negative regulation of transcription, DNA-templated
inferred from mutant phenotype
(Lim et al., 2012)
involved_in neuron cellular homeostasis
inferred from mutant phenotype
(Lim et al., 2012)
involved_in photoreceptor cell development
inferred from mutant phenotype
(Tsuda et al., 2006, Tsuda et al., 2002)
involved_in positive regulation of histone deacetylation
inferred from direct assay
(Qi et al., 2008)
involved_in positive regulation of imaginal disc growth
inferred from mutant phenotype
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:aos; FB:FBgn0004569
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:Pi3K92E; FB:FBgn0015279
(Marygold et al., 2011)
involved_in positive regulation of Notch signaling pathway
inferred from genetic interaction with FLYBASE:Dl; FB:FBgn0000463
(Tsuda et al., 2002)
inferred from genetic interaction with FLYBASE:Ser; FB:FBgn0004197
(Tsuda et al., 2002)
inferred from genetic interaction with FLYBASE:N; FB:FBgn0004647
(Marygold et al., 2011, Tsuda et al., 2002)
inferred from direct assay
(Marygold et al., 2011)
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process
inferred from direct assay
(Boulton et al., 2000)
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process
inferred from mutant phenotype
(Nguyen et al., 2016)
involved_in regulation of epidermal growth factor receptor signaling pathway
inferred from mutant phenotype
(assigned by UniProt )
(Dong et al., 1999)
involved_in response to oxidative stress
inferred from mutant phenotype
(Lim et al., 2012)
involved_in wing disc development
inferred from mutant phenotype
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:sd; FB:FBgn0003345
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:vg; FB:FBgn0003975
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:N; FB:FBgn0004647
(Marygold et al., 2011)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in histone deacetylation
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(assigned by UniProt )
(Dong et al., 1999)
inferred from high throughput direct assay
(Sarov et al., 2016)
located_in polytene chromosome
inferred from direct assay
(Marygold et al., 2011)
part_of transcription repressor complex
inferred from genetic interaction with FLYBASE:Su(H); FB:FBgn0004837
(Tsuda et al., 2006)
inferred from physical interaction with FLYBASE:Smr; FB:FBgn0265523
(Tsuda et al., 2006)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of histone deacetylase complex
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
Belongs to the WD repeat EBI family. (Q95RJ9)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
ebi (ebi) encodes a nuclear-localized protein that regulates Egfr, Notch and Wg signaling pathways. ebi encoded product contributes to multiple processes including wing growth and eye development as well as regulation of transcription and innate immune response. [Date last reviewed: 2019-03-07]
Pathway (FlyBase)
Positive Regulators of Notch Signaling Pathway -
The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
EGFR Signaling Pathway Core Components -
The Epidermal Growth Factor Receptor (EGFR) signaling pathway is used multiple times during development (FBrf0190321). It is activated by the binding of a secreted ligand to the receptor tyrosine kinase Egfr and acts via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0190321 and FBrf0221727).
Protein Function (UniProtKB)
F-box-like component of E3 ubiquitin ligase complexes; involved in R7 photoreceptor cell differentiation, cone cell development and neuronal cell cycle control. E3 ubiquitin ligase complexes mediate ubiquitination and subsequent proteasomal degradation of target proteins. Required for specification of R7 photoreceptor cell fate in the eye by participating in the ubiquitination and subsequent proteasomal degradation of Tramtrack (ttk), a general inhibitor of photoreceptor differentiation. Required to block the S phase entry in the peripheral nervous system and central nervous system in a process that does not involve the degradation of ttk. Involved in cone cell development by preventing the transcriptional repression mediated by Su(H) on Dl, probably by participating in a E3 complex that contains sno and mediates the ubiquitination and subsequent proteasomal degradation of some component of the Su(H) repressor complex.
(UniProt, Q95RJ9)
Summary (Interactive Fly)

Trp-Asp (WD) repeats protein - regulates Egfr pathway & protein degradation - cellular defense and sensory cell survival require distinct functions of - modulates wing growth by ubiquitin-dependent downregulation of Crumbs

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\ebi for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene
gene
(Coelho et al., 2005)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
ebi-RA
FBtr0078055
NM_057981
3327
700
Additional Transcript Data and Comments
Reported size (kB)

3.349 (longest cDNA)

(Dong et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
ebi-PA
FBpp0077715
72.4
700
5.72
NP_477329
AAF51501
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

700 (aa)

(Dong et al., 1999)
Comments
External Data
Subunit Structure (UniProtKB)

Component of some E3 complex at least composed of sina, ebi and phyl, required for the degradation of ttk. Probably forms a E3 complex with sno, required for the degradation of some component of the Su(H) repressor complex. Interacts with sno and Su(H) and Smr.

(UniProt, Q95RJ9)
Domain

The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.

(UniProt, Q95RJ9)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ebi using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
salivary gland
(Dong et al., 1999)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- larval stage
organism
• nucleus
(Dong et al., 1999)
third instar larval stage
eye disc
• nucleus
(Dong et al., 1999)
Additional Descriptive Data

ebi protein is localized to the nucleus of all embryonic and larval cells examined. Structural analysis using myc-tagged ebi protein reveals that is distributed in a reticular network pattern in the nucleoplasm, and is not associated with the chromosomes or nucleolus.

(Dong et al., 1999)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(assigned by UniProt )
(Dong et al., 1999)
inferred from high throughput direct assay
(Sarov et al., 2016)
located_in polytene chromosome
inferred from direct assay
(Marygold et al., 2011)
part_of transcription repressor complex
inferred from genetic interaction with FLYBASE:Su(H); FB:FBgn0004837
(Tsuda et al., 2006)
inferred from physical interaction with FLYBASE:Smr; FB:FBgn0265523
(Tsuda et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ebi in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 18 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ebi
Transgenic constructs containing regulatory region of ebi
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(2L)al
(Marygold et al., 2011, Dong et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)
Inferred from location ( 0 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
cell lethal | larval stage | somatic clone
lethal
lethal, with Scer\GAL4Act5C.PI
lethal, with Scer\GAL4Act5C.PU
lethal, with Scer\GAL4en.PU
lethal, with Scer\GAL4ey.PH
lethal (with Df(2L)al)
lethal (with ebi2g1)
lethal (with ebi2g2)
lethal (with ebiCCS-8)
lethal (with ebiE90)
lethal (with ebik16213)
lethal (with ebiP7)
lethal (with ebiWKS-24)
lethal | male, with Scer\GAL4GMR.PF, Scer\GAL4ey.PH
lethal | pupal stage, with Scer\GAL4pnr-MD237
lethal | recessive
lethal - all die before end of larval stage (with ebi2g1)
lethal - all die before end of larval stage (with ebi2g2)
lethal - all die before end of pupal stage, with Scer\GAL4da.G32
lethal - all die during embryonic stage | recessive
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
partially lethal - majority die
partially lethal - majority die | female, with Scer\GAL4GMR.PF, Scer\GAL4ey.PH
partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Bx-MS1096
viable, with Scer\GAL4en.PU
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female semi-sterile | recessive
Other Phenotypes
Allele
abnormal mitotic cell cycle | embryonic stage 13
abnormal neuroanatomy | adult stage | progressive
abnormal neuroanatomy | adult stage | progressive | somatic clone
abnormal oxidative stress response (with ebiE90)
abnormal oxidative stress response (with ebiP7)
abnormal size
abnormal size, with Scer\GAL4Bx-MS1096
abnormal size, with Scer\GAL4en.PU
abnormal size | adult stage, with Scer\GAL4bbg-C96
abnormal size | adult stage, with Scer\GAL4nub.PK
abnormal size | adult stage, with Scer\GAL4nub.PU
decreased body size | larval stage (with ebi2g1)
decreased body size | larval stage (with ebi2g2)
decreased cell number | embryonic stage
increased occurrence of cell division | embryonic stage
some die during embryonic stage | recessive
some die during larval stage (with ebi2g1)
some die during larval stage (with ebi2g2)
some die during pupal stage, with Scer\GAL4da.G32
visible, with Scer\GAL4pnr-MD237
visible | adult stage, with Scer\GAL4bbg-C96
visible | adult stage, with Scer\GAL4nub.PK
visible | adult stage, with Scer\GAL4nub.PU
visible | recessive
Phenotype manifest in
Allele
antenna
cone cell
cone cell | somatic clone
crossvein, with Scer\GAL4nub.PU
crystal cell
denticle belt
embryo
embryonic epidermis
eye
eye, with Scer\GAL4GMR.PF, Scer\GAL4ey.PH
eye | somatic clone
eye photoreceptor cell | somatic clone
filzkorper
leg
macrochaeta
macrochaeta | ectopic
mesothoracic tergum, with Scer\GAL4pnr-MD237
mitochondrion | progressive
mitotic cell cycle
neuron | embryonic stage
ommatidium
ommatidium | somatic clone
photoreceptor cell
presumptive embryonic/larval peripheral nervous system
retina | progressive
retina | progressive | somatic clone | conditional
thorax, with Scer\GAL4pnr-MD237
trichogen cell, with Scer\GAL4pnr-MD237
wing
wing, with Scer\GAL4bbg-C96
wing, with Scer\GAL4Bx-MS1096
wing, with Scer\GAL4en.PU
wing, with Scer\GAL4nub.PK
wing, with Scer\GAL4nub.PU
wing margin
wing pouch
wing pouch, with Scer\GAL4Bx-MS1096
wing pouch, with Scer\GAL4en.PU
wing vein
wing vein, with Scer\GAL4nub.PK
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\TBL1X
15 of 15
Yes
Yes
1  
Hsap\TBL1XR1
14 of 15
No
Yes
Hsap\TBL1Y
11 of 15
No
Yes
1  
Hsap\WDR17
1 of 15
No
Yes
Hsap\WDR38
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Tbl1x
14 of 15
Yes
Yes
Mmus\Tbl1xr1
13 of 15
No
Yes
Mmus\Wdr17
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
Rnor\Tbl1xr1
12 of 13
Yes
Yes
Rnor\Tbl1x
12 of 13
Yes
Yes
Rnor\Wdr17
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
Xtro\tbl1x
9 of 12
Yes
Yes
Xtro\tbl1xr1
8 of 12
No
Yes
Xtro\wdr17
1 of 12
No
Yes
Danio rerio (Zebrafish) (4)
Drer\tbl1x
13 of 15
Yes
Yes
Drer\tbl1xr1a
12 of 15
No
Yes
Drer\tbl1xr1b
12 of 15
No
Yes
Drer\wdr17
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\unc-37
1 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (1)
Atha\HOS15
7 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\SIF2
10 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\hif2
6 of 12
Yes
Yes
Spom\tup12
1 of 12
No
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091905PX )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000040
Drosophila simulans
Dsim\GD23022
Drosophila sechellia
Dsec\GM16739
Drosophila erecta
Dere\GG24716
Drosophila yakuba
Dyak\GE16791
Drosophila ananassae
Dana\GF20651
Drosophila pseudoobscura pseudoobscura
Dpse\GA17928
Drosophila persimilis
Dper\GL18875
Drosophila willistoni
Dwil\GK24403
Drosophila virilis
Dvir\GJ17774
Drosophila mojavensis
Dmoj\GI21646
Drosophila grimshawi
Dgri\GH11222
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503CJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17009920
Glossina morsitans
Tsetse fly
Gmor\GMOY001506
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_09401
Mayetiola destructor
Hessian fly
Mdes\Mdes015676
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL003539
Anopheles darlingi
American malaria mosquito
Adar\ADAC003732
Anopheles gambiae
Malaria mosquito
Agam\AGAP007739
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ010919
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0349 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA007583
Danaus plexippus
Monarch butterfly
Dple\DPOGS204225-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL015146
Apis florea
Little honeybee
Aflo\3740288644
Apis mellifera
Western honey bee
Amel\ebi
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22598
Bombus terrestris
Buff-tailed bumblebee
Bter\15201839
Linepithema humile
Argentine ant
Lhum\LH12143
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001930
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG002461
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_06179
Tribolium castaneum
Red flour beetle
Tcas\TC003706
Pediculus humanus
Human body louse
Phum\PHUM076530
Rhodnius prolixus
Kissing bug
Rpro\RPRC005791
Cimex lectularius
Bed bug
Clec\CLEC009857
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI009292
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI004972
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03403
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0313 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR015176
Strigamia maritima
European centipede
Smar\SMAR015788
Ixodes scapularis
Black-legged tick
Isca\ISCW008840
Stegodyphus mimosarum
African social velvet spider
Smim\KFM79246.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur05g03980
Tetranychus urticae
Two-spotted spider mite
Turt\tetur05g03990
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_224492
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_253694
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_53261
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_271400
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04MW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-TbL1xr1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_005499gn
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000008885
Gallus gallus
Domestic chicken
Ggal\TBL1XR1
Gallus gallus
Domestic chicken
Ggal\TBL1X
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Evidence
    References
    ebi1-334.GMR
    model of  sensory system disease
    CEA
    (Lim et al., 2012)
    ebik16213
    model of  sensory system disease
    CEA
    (Lim et al., 2012)
    Potential Models Based on Orthology ( 3 )
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    ebik16213
    ameliorates  multiple endocrine neoplasia type 2B
    modeled by RetMEN2B.GMR
    (Read et al., 2005)
    ameliorates  multiple endocrine neoplasia type 2A
    modeled by RetMEN2A.GMR
    (Read et al., 2005)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    TBL1X; transducin beta like 1 X-linked
    15 of 15
    300196
     
    TBL1XR1; TBL1X receptor 1
    14 of 15
    608628
    TBL1Y; transducin beta like 1 Y-linked
    11 of 15
    400033
     
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    ebi - Bap60
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - CG17002
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - CG2017
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - CG3008
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - CG34159
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - Cdk8
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - Cpsf6
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - CtBP
    pull down, autoradiography
    (Qi et al., 2008)
    ebi - HDAC3
    anti bait coimmunoprecipitation, western blot
    (Qi et al., 2008)
    ebi - Jra
    anti tag coimmunoprecipitation, anti tag western blot
    (Lim et al., 2012)
    ebi - Larp4B
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED10
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED11
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED14
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED15
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED16
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED17
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED18
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED20
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED23
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED25
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED27
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED30
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED4
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED6
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED7
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - MED8
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - Pa1
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - Pc
    pull down, peptide massfingerprinting
    (Strübbe et al., 2011)
    ebi - Psi
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - Ptip
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - Rbf
    anti tag coimmunoprecipitation, anti tag western blot
    (Lim et al., 2013)
    ebi - Sec23
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - Sec24AB
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - Smyd4-2
    anti tag coimmunoprecipitation, anti tag western blot
    (Thompson and Travers, 2008)
    ebi - Snr1
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - Ssdp
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - Usp10
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - boca
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - brm
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - crb
    anti tag coimmunoprecipitation, anti tag western blot, pull down, autoradiography, two hybrid
    (Nguyen et al., 2016)
    ebi - dsh
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - enc
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - ix
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - mor
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - osa
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - sina
    anti tag coimmunoprecipitation, anti tag western blot, pull down, autoradiography
    (Cooper, 2007)
    ebi - sinah
    pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot
    (Cooper, 2007)
    ebi - skd
    experimental knowledge based
    (Guruharsha et al., 2011)
    ebi - sna
    pull down, autoradiography
    (Qi et al., 2008)
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    ebi
    suppressible
    crb
    (Nguyen et al., 2016)
    ebi
    enhanceable
    Dcr-2
    (Dietzl et al., 2007)
    ebi
    enhanceable
    Diap1
    (Lim et al., 2012)
    ebi
    enhanceable
    Dl
    (Tsuda et al., 2002)
    ebi
    suppressible
    Dl
    (Tsuda et al., 2002)
    ebi
    suppressible
    hep
    (Lim et al., 2012)
    ebi
    suppressible
    Jra
    (Lim et al., 2012)
    ebi
    enhanceable
    puc
    (Lim et al., 2012)
    ebi
    suppressible
    rno
    (Voas and Rebay, 2003)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    aos
    enhanceable
    ebi
    (Marygold et al., 2011, Coelho et al., 2005)
    chn
    enhanceable
    ebi
    (Tsuda et al., 2006)
    crb
    suppressible
    ebi
    (Nguyen et al., 2016)
    Dl
    enhanceable
    ebi
    (Tsuda et al., 2002)
    Dp|E2f1
    enhanceable
    ebi
    (Boulton et al., 2000)
    Egfr::tor
    suppressible
    ebi
    (Dong et al., 1999)
    Egfr
    enhanceable
    ebi
    (Dong et al., 1999)
    egr
    enhanceable
    ebi
    (Lim et al., 2012)
    N
    enhanceable
    ebi
    (Marygold et al., 2011, Tsuda et al., 2002)
    Pc
    suppressible
    ebi
    (Strübbe et al., 2011)
    Pi3K92E
    enhanceable
    ebi
    (Marygold et al., 2011, Coelho et al., 2005)
    Ret
    suppressible
    ebi
    (Read et al., 2005)
    rux
    enhanceable
    ebi
    (Dong et al., 1999)
    sd
    enhanceable
    ebi
    (Marygold et al., 2011)
    Ser
    enhanceable
    ebi
    (Tsuda et al., 2002)
    sev|Sos
    enhanceable
    ebi
    (Dong et al., 1999)
    sno
    enhanceable
    ebi
    (Tsuda et al., 2002)
    External Data
    Subunit Structure (UniProtKB)
    Component of some E3 complex at least composed of sina, ebi and phyl, required for the degradation of ttk. Probably forms a E3 complex with sno, required for the degradation of some component of the Su(H) repressor complex. Interacts with sno and Su(H) and Smr.
    (UniProt, Q95RJ9 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Positive Regulators of Notch Signaling Pathway -
    The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
    EGFR Signaling Pathway Core Components -
    The Epidermal Growth Factor Receptor (EGFR) signaling pathway is used multiple times during development (FBrf0190321). It is activated by the binding of a secreted ligand to the receptor tyrosine kinase Egfr and acts via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0190321 and FBrf0221727).
    Metabolic Pathways
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-0.7
    Cytogenetic map
    21C2-21C2
    Sequence location
    2L:415,067..418,536 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    21C2-21C2
    Limits computationally determined from genome sequence between P{lacW}RpI135k16513&P{lacW}ebik16213 and P{lacW}l(2)k09610k09610&P{lacW}Sk09538a
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    21B-21D
    (Dong, 1999.4.27)
    21C2-21C3
    (determined by in situ hybridisation)
    (Spradling et al., 1999)
    21C2-21C3
    (BDGP Project Members, 1994-1999)
    Experimentally Determined Recombination Data
    Location

    2-0.7

    (FlyBase, 2016)

    2-0.0

    (Comeron et al., 2012)

    2-

    (Coelho et al., 2005)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (24)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (120)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: ebi CG4063

    (Guan, 1999.11)
    Source for database merge of

    Source for merge of: ebi l(2)k16213

    (Dong et al., 1999)

    Source for merge of: ebi E-2g

    (Marygold et al., 2011)
    Additional comments
    Other Comments

    Phenotypes in trans with ebiP7 and genetic interactions order ebi mutations into the following allelic series (strongest to weakest): ebi2g2, ebiE90, ebiCC3, ebi2g1, ebiCCS-8, ebiE4, ebiΔ1, ebiW418X, ebik16213, ebiWKS-24, ebiP7.

    (Marygold et al., 2011)

    Identification: as a complementation group whose members dominantly modify the small wing phenotype caused by expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096. 2 alleles of E-2g have been recovered.

    (Coelho et al., 2005)

    sno and ebi play a key role in linking the activation of the Egfr pathway to the expression of Dl in R cells by inactivation of Su(H)-mediated repression.

    (Tsuda et al., 2002)

    ebi has two distinct functions; it promotes the degradation of a repressor of neuronal differentiation ttk88, and has a second independant function that limits S phase entry.

    (Boulton et al., 2000)

    ebi is required for Egfr but not tor-receptor-dependent gene expression.

    (Dong et al., 1999)

    May function in MAPK signaling pathways.

    (Tsuda et al., 1997)

    Mutations do not show genetic interactions with tor or sev mutations.

    (Tsuda et al., 1997)
    Origin and Etymology
    Discoverer
    Etymology

    'ebi' means 'shrimp' in Japanese.

    (Dong et al., 1999)
    Identification
    External Crossreferences and Linkouts ( 42 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    ApoDroso - Functional genomic database for photoreceptor development, survival and function
    DroID - A comprehensive database of gene and protein interactions.
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (11)
    Reported As
    Symbol Synonym
    CG4063
    (Kondo et al., 2017, Hosono et al., 2015, Roberts et al., 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Grieder et al., 2007, Brandt, 2006)
    E-2f
    (Coelho et al., 2005)
    E-2g
    Ebi
    (Nguyen et al., 2016, Fiedler et al., 2015, Mannervik, 2014, Roberts et al., 2012, Strübbe et al., 2011, Thompson and Travers, 2008, Cooper, 2007, Tsuda et al., 2006, Goode et al., 2005, Hall et al., 2005, Sundaram, 2005, Tsuda et al., 2005, Voas and Rebay, 2004, Dong, 1999.4.27)
    FBXW4
    (Roberts et al., 2012)
    Tb11
    (Spradling et al., 1999)
    Tbl1
    (Spradling et al., 1999)
    ebi
    (Tsui et al., 2018, Houtz et al., 2017, Kondo et al., 2017, Lim and Tsuda, 2016, Nguyen et al., 2016, Sarov et al., 2016, Dietz et al., 2015, Lim et al., 2015, Toret et al., 2014, Das et al., 2013, Debruhl et al., 2013, Handler et al., 2013, Kockmann et al., 2013, Lim et al., 2013, Wang et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Lim et al., 2012, Murray et al., 2012, Roberts et al., 2012, Friedman et al., 2011, Marygold et al., 2011, Strübbe et al., 2011, Toku et al., 2011, Inomata et al., 2008, Qi et al., 2008, Zhao et al., 2008, Dietzl et al., 2007, Kaplow et al., 2007, Nagaraj and Banerjee, 2007, Brandt, 2006, Boulton et al., 2000)
    l(2)k16213
    Name Synonyms
    Ebi
    (Lim and Thiery, 2012, Cooper, 2007)
    Enhancer on chromosome 2-complementation group 2g
    (Coelho et al., 2005)
    beta transducin-like 1 protein
    (Spradling et al., 1999)
    ebi
    (Dong et al., 1999)
    Secondary FlyBase IDs
    • FBgn0023444
    • FBgn0021820
    • FBgn0085068
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (108)