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General Information
Symbol
Dmel\ebi
Species
D. melanogaster
Name
ebi
Annotation Symbol
CG4063
Feature Type
FlyBase ID
FBgn0263933
Gene Model Status
Stock Availability
Gene Snapshot
ebi (ebi) encodes a nuclear-localized protein that regulates Egfr, Notch and Wg signaling pathways. ebi encoded product contributes to multiple processes including wing growth and eye development as well as regulation of transcription and innate immune response. [Date last reviewed: 2019-03-07]
Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:415,067..418,536 [+]
Recombination map
2-0.7
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the WD repeat EBI family. (Q95RJ9)
Summaries
Pathway (FlyBase)
Positive Regulators of Notch Signaling Pathway -
The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
Protein Function (UniProtKB)
F-box-like component of E3 ubiquitin ligase complexes; involved in R7 photoreceptor cell differentiation, cone cell development and neuronal cell cycle control. E3 ubiquitin ligase complexes mediate ubiquitination and subsequent proteasomal degradation of target proteins. Required for specification of R7 photoreceptor cell fate in the eye by participating in the ubiquitination and subsequent proteasomal degradation of Tramtrack (ttk), a general inhibitor of photoreceptor differentiation. Required to block the S phase entry in the peripheral nervous system and central nervous system in a process that does not involve the degradation of ttk. Involved in cone cell development by preventing the transcriptional repression mediated by Su(H) on Dl, probably by participating in a E3 complex that contains sno and mediates the ubiquitination and subsequent proteasomal degradation of some component of the Su(H) repressor complex.
(UniProt, Q95RJ9)
Summary (Interactive Fly)
Trp-Asp (WD) repeats protein - regulates Egfr pathway & protein degradation - cellular defense and sensory cell survival require distinct functions of - modulates wing growth by ubiquitin-dependent downregulation of Crumbs
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\ebi or the JBrowse view of Dmel\ebi for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.44
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0078055
3327
700
Additional Transcript Data and Comments
Reported size (kB)
3.349 (longest cDNA)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0077715
72.4
700
5.72
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Component of some E3 complex at least composed of sina, ebi and phyl, required for the degradation of ttk. Probably forms a E3 complex with sno, required for the degradation of some component of the Su(H) repressor complex. Interacts with sno and Su(H) and Smr.
(UniProt, Q95RJ9)
Domain
The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
(UniProt, Q95RJ9)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ebi using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (25 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from physical interaction with UniProtKB:Q27934
(assigned by UniProt )
inferred from physical interaction with FLYBASE:inferred from high throughput direct assayC3; FB:FBgn0025825
inferred from physical interaction with FLYBASE:Smyd4-2; FB:FBgn0036282
inferred from physical interaction with FLYBASE:sna; FB:FBgn0003448
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
Biological Process (17 terms)
Terms Based on Experimental Evidence (15 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Jra; FB:FBgn0001291
inferred from genetic interaction with FLYBASE:hep; FB:FBgn0010303
inferred from genetic interaction with FLYBASE:egr; FB:FBgn0033483
inferred from genetic interaction with FLYBASE:puc; FB:FBgn0243512
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:aos; FB:FBgn0004569
inferred from genetic interaction with FLYBASE:Pi3K92E; FB:FBgn0015279
inferred from direct assay
inferred from genetic interaction with FLYBASE:N; FB:FBgn0004647
inferred from genetic interaction with FLYBASE:Dl; FB:FBgn0000463
inferred from genetic interaction with FLYBASE:Ser; FB:FBgn0004197
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:sd; FB:FBgn0003345
inferred from genetic interaction with FLYBASE:vg; FB:FBgn0003975
inferred from genetic interaction with FLYBASE:N; FB:FBgn0004647
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from high throughput direct assay
inferred from direct assay
inferred from genetic interaction with FLYBASE:Su(H); FB:FBgn0004837
inferred from physical interaction with FLYBASE:Smr; FB:FBgn0265523
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
ebi protein is localized to the nucleus of all embryonic and larval cells examined. Structural analysis using myc-tagged ebi protein reveals that is distributed in a reticular network pattern in the nucleoplasm, and is not associated with the chromosomes or nucleolus.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from high throughput direct assay
inferred from direct assay
inferred from genetic interaction with FLYBASE:Su(H); FB:FBgn0004837
inferred from physical interaction with FLYBASE:Smr; FB:FBgn0265523
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ebi in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 18 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ebi
Transgenic constructs containing regulatory region of ebi
Deletions and Duplications ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
13 of 15
No
Yes
12 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
13 of 15
Yes
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
11 of 13
Yes
Yes
11 of 13
Yes
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
8 of 12
Yes
Yes
7 of 12
No
Yes
Danio rerio (Zebrafish) (3)
13 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
1 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (1)
6 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
10 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
7 of 12
Yes
Yes
1 of 12
No
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091905PX )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503CJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0349 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0313 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04MW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Component of some E3 complex at least composed of sina, ebi and phyl, required for the degradation of ttk. Probably forms a E3 complex with sno, required for the degradation of some component of the Su(H) repressor complex. Interacts with sno and Su(H) and Smr.
    (UniProt, Q95RJ9 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    Positive Regulators of Notch Signaling Pathway -
    The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-0.7
    Cytogenetic map
    Sequence location
    2L:415,067..418,536 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    21C2-21C2
    Limits computationally determined from genome sequence between P{lacW}RpI135k16513&P{lacW}ebik16213 and P{lacW}l(2)k09610k09610&P{lacW}Sk09538a
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    21C2-21C3
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (26)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (120)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: ebi CG4063
    Source for database merge of
    Source for merge of: ebi l(2)k16213
    Source for merge of: ebi E-2g
    Additional comments
    Other Comments
    Phenotypes in trans with ebiP7 and genetic interactions order ebi mutations into the following allelic series (strongest to weakest): ebi2g2, ebiE90, ebiCC3, ebi2g1, ebiCCS-8, ebiE4, ebiΔ1, ebiW418X, ebik16213, ebiWKS-24, ebiP7.
    Identification: as a complementation group whose members dominantly modify the small wing phenotype caused by expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096. 2 alleles of E-2g have been recovered.
    sno and ebi play a key role in linking the activation of the Egfr pathway to the expression of Dl in R cells by inactivation of Su(H)-mediated repression.
    ebi has two distinct functions; it promotes the degradation of a repressor of neuronal differentiation ttk88, and has a second independant function that limits S phase entry.
    ebi is required for Egfr but not tor-receptor-dependent gene expression.
    May function in MAPK signaling pathways.
    Mutations do not show genetic interactions with tor or sev mutations.
    Origin and Etymology
    Discoverer
    Etymology
    'ebi' means 'shrimp' in Japanese.
    Identification
    External Crossreferences and Linkouts ( 45 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    ApoDroso - Functional genomic database for photoreceptor development, survival and function
    DroID - A comprehensive database of gene and protein interactions.
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (11)
    Reported As
    Name Synonyms
    Enhancer on chromosome 2-complementation group 2g
    beta transducin-like 1 protein
    Secondary FlyBase IDs
    • FBgn0023444
    • FBgn0021820
    • FBgn0085068
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (106)