FB2020_01, released Feb 12, 2020

Gene: Dmel\ebi

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General Information
Symbol
Dmel\ebi
Species
D. melanogaster
Name
ebi
Annotation Symbol
CG4063
Feature Type
protein_coding_gene
FlyBase ID
FBgn0263933
Gene Model Status
Current
Stock Availability
25 publicly available
Gene Snapshot
ebi (ebi) encodes a nuclear-localized protein that regulates Egfr, Notch and Wg signaling pathways. ebi encoded product contributes to multiple processes including wing growth and eye development as well as regulation of transcription and innate immune response. [Date last reviewed: 2019-03-07]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
21C2-21C2
Sequence location
2L:415,067..418,536 [+]
Recombination map

2-0.7

RefSeq locus
NT_033779 REGION:415067..418536
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Pathway (FlyBase)
Protein Family (UniProt)
Belongs to the WD repeat EBI family. (Q95RJ9)
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
chromatin binding; protein binding; repressing transcription factor binding; contributes_to transcription corepressor activity
Predictions / Assertions
-
Summaries
Pathway (FlyBase)
Positive Regulators of Notch Signaling Pathway -
The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
Protein Function (UniProtKB)
F-box-like component of E3 ubiquitin ligase complexes; involved in R7 photoreceptor cell differentiation, cone cell development and neuronal cell cycle control. E3 ubiquitin ligase complexes mediate ubiquitination and subsequent proteasomal degradation of target proteins. Required for specification of R7 photoreceptor cell fate in the eye by participating in the ubiquitination and subsequent proteasomal degradation of Tramtrack (ttk), a general inhibitor of photoreceptor differentiation. Required to block the S phase entry in the peripheral nervous system and central nervous system in a process that does not involve the degradation of ttk. Involved in cone cell development by preventing the transcriptional repression mediated by Su(H) on Dl, probably by participating in a E3 complex that contains sno and mediates the ubiquitination and subsequent proteasomal degradation of some component of the Su(H) repressor complex.
(UniProt, Q95RJ9)
Summary (Interactive Fly)

Trp-Asp (WD) repeats protein - regulates Egfr pathway & protein degradation - cellular defense and sensory cell survival require distinct functions of - modulates wing growth by ubiquitin-dependent downregulation of Crumbs

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\ebi or the JBrowse view of Dmel\ebi for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene
gene
(Coelho et al., 2005)
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
ebi-RA
FBtr0078055
NM_057981
3327
700
Additional Transcript Data and Comments
Reported size (kB)

3.349 (longest cDNA)

(Dong et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
ebi-PA
FBpp0077715
72.4
700
5.72
NP_477329
AAF51501
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

700 (aa)

(Dong et al., 1999)
Comments
External Data
Subunit Structure (UniProtKB)

Component of some E3 complex at least composed of sina, ebi and phyl, required for the degradation of ttk. Probably forms a E3 complex with sno, required for the degradation of some component of the Su(H) repressor complex. Interacts with sno and Su(H) and Smr.

(UniProt, Q95RJ9)
Domain

The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.

(UniProt, Q95RJ9)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ebi using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (25 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
chromatin binding
inferred from direct assay
(Tsuda et al., 2006)
protein binding
inferred from physical interaction with UniProtKB:Q27934
(assigned by UniProt )
(Boulton et al., 2000)
inferred from physical interaction with FLYBASE:inferred from high throughput direct assayC3; FB:FBgn0025825
(Qi et al., 2008)
inferred from physical interaction with FLYBASE:Smyd4-2; FB:FBgn0036282
(Thompson and Travers, 2008)
repressing transcription factor binding
inferred from physical interaction with FLYBASE:sna; FB:FBgn0003448
(Qi et al., 2008)
contributes_to transcription corepressor activity
inferred from physical interaction with FLYBASE:sna; FB:FBgn0003448
(Qi et al., 2008)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (17 terms)
Terms Based on Experimental Evidence (15 terms)
CV Term
Evidence
References
cellular response to light stimulus
inferred from mutant phenotype
(Lim et al., 2012)
chaeta development
inferred from mutant phenotype
(Marygold et al., 2011)
imaginal disc-derived wing margin morphogenesis
inferred from mutant phenotype
(Nguyen et al., 2016)
negative regulation of JNK cascade
inferred from genetic interaction with FLYBASE:Jra; FB:FBgn0001291
(Lim et al., 2012)
inferred from genetic interaction with FLYBASE:hep; FB:FBgn0010303
(Lim et al., 2012)
inferred from genetic interaction with FLYBASE:egr; FB:FBgn0033483
(Lim et al., 2012)
inferred from genetic interaction with FLYBASE:puc; FB:FBgn0243512
(Lim et al., 2012)
negative regulation of neuron apoptotic process
inferred from mutant phenotype
(Lim et al., 2012)
negative regulation of transcription, DNA-templated
inferred from mutant phenotype
(Lim et al., 2012)
neuron cellular homeostasis
inferred from mutant phenotype
(Lim et al., 2012)
photoreceptor cell development
inferred from mutant phenotype
(Tsuda et al., 2006, Tsuda et al., 2002)
positive regulation of histone deacetylation
inferred from direct assay
(Qi et al., 2008)
positive regulation of imaginal disc growth
inferred from mutant phenotype
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:aos; FB:FBgn0004569
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:Pi3K92E; FB:FBgn0015279
(Marygold et al., 2011)
positive regulation of Notch signaling pathway
inferred from direct assay
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:N; FB:FBgn0004647
(Marygold et al., 2011, Tsuda et al., 2002)
inferred from genetic interaction with FLYBASE:Dl; FB:FBgn0000463
(Tsuda et al., 2002)
inferred from genetic interaction with FLYBASE:Ser; FB:FBgn0004197
(Tsuda et al., 2002)
proteasome-mediated ubiquitin-dependent protein catabolic process
inferred from mutant phenotype
(Nguyen et al., 2016)
regulation of epidermal growth factor receptor signaling pathway
inferred from mutant phenotype
(assigned by UniProt )
(Dong et al., 1999)
response to oxidative stress
inferred from mutant phenotype
(Lim et al., 2012)
wing disc development
inferred from mutant phenotype
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:sd; FB:FBgn0003345
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:vg; FB:FBgn0003975
(Marygold et al., 2011)
inferred from genetic interaction with FLYBASE:N; FB:FBgn0004647
(Marygold et al., 2011)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
histone deacetylation
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
(Gaudet et al., 2011)
regulation of transcription by RNA polymerase II
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
nucleus
inferred from direct assay
(assigned by UniProt )
(Dong et al., 1999)
inferred from high throughput direct assay
(Sarov et al., 2016)
polytene chromosome
inferred from direct assay
(Marygold et al., 2011)
transcriptional repressor complex
inferred from genetic interaction with FLYBASE:Su(H); FB:FBgn0004837
(Tsuda et al., 2006)
inferred from physical interaction with FLYBASE:Smr; FB:FBgn0265523
(Tsuda et al., 2006)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
histone deacetylase complex
inferred from biological aspect of ancestor with PANTHER:PTN000521099
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
salivary gland
(Dong et al., 1999)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- larval stage
organism
• nucleus
(Dong et al., 1999)
third instar larval stage
eye disc
• nucleus
(Dong et al., 1999)
Additional Descriptive Data

ebi protein is localized to the nucleus of all embryonic and larval cells examined. Structural analysis using myc-tagged ebi protein reveals that is distributed in a reticular network pattern in the nucleoplasm, and is not associated with the chromosomes or nucleolus.

(Dong et al., 1999)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
nucleus
inferred from direct assay
(assigned by UniProt )
(Dong et al., 1999)
inferred from high throughput direct assay
(Sarov et al., 2016)
polytene chromosome
inferred from direct assay
(Marygold et al., 2011)
transcriptional repressor complex
inferred from genetic interaction with FLYBASE:Su(H); FB:FBgn0004837
(Tsuda et al., 2006)
inferred from physical interaction with FLYBASE:Smr; FB:FBgn0265523
(Tsuda et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\ebi in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 20 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 18 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ebi
Transgenic constructs containing regulatory region of ebi
Deletions and Duplications ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
cell lethal | larval stage | somatic clone
lethal
lethal, with Scer\GAL4Act5C.PI
lethal, with Scer\GAL4Act5C.PU
lethal, with Scer\GAL4en.PU
lethal, with Scer\GAL4ey.PH
lethal (with Df(2L)al)
lethal (with ebi2g1)
lethal (with ebi2g2)
lethal (with ebiCCS-8)
lethal (with ebiE90)
lethal (with ebik16213)
lethal (with ebiP7)
lethal (with ebiWKS-24)
lethal | male, with Scer\GAL4GMR.PF, Scer\GAL4ey.PH
lethal | pupal stage, with Scer\GAL4pnr-MD237
lethal | recessive
lethal - all die before end of embryonic stage | recessive
lethal - all die before end of larval stage (with ebi2g1)
lethal - all die before end of larval stage (with ebi2g2)
lethal - all die before end of pupal stage, with Scer\GAL4da.G32
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
partially lethal - majority die
partially lethal - majority die | female, with Scer\GAL4GMR.PF, Scer\GAL4ey.PH
partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Bx-MS1096
viable, with Scer\GAL4en.PU
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female semi-sterile | recessive
Other Phenotypes
Allele
decreased cell number | embryonic stage
increased occurrence of cell division | embryonic stage
mitotic cell cycle defective | embryonic stage 13
neuroanatomy defective | adult stage | progressive
neuroanatomy defective | adult stage | progressive | somatic clone
oxidative stress response defective (with ebiE90)
oxidative stress response defective (with ebiP7)
size defective
size defective, with Scer\GAL4Bx-MS1096
size defective, with Scer\GAL4en.PU
size defective | adult stage, with Scer\GAL4bbg-C96
size defective | adult stage, with Scer\GAL4nub.PK
size defective | adult stage, with Scer\GAL4nub.PU
small body | larval stage (with ebi2g1)
small body | larval stage (with ebi2g2)
some die during embryonic stage | recessive
some die during larval stage (with ebi2g1)
some die during larval stage (with ebi2g2)
some die during pupal stage, with Scer\GAL4da.G32
visible, with Scer\GAL4pnr-MD237
visible | adult stage, with Scer\GAL4bbg-C96
visible | adult stage, with Scer\GAL4nub.PK
visible | adult stage, with Scer\GAL4nub.PU
visible | recessive
Phenotype manifest in
Allele
antenna
cone cell
cone cell | somatic clone
crossvein, with Scer\GAL4nub.PU
crystal cell
denticle belt
embryo
embryonic epidermis
eye
eye, with Scer\GAL4GMR.PF, Scer\GAL4ey.PH
eye | somatic clone
eye photoreceptor cell | somatic clone
filzkorper
leg
macrochaeta
macrochaeta | ectopic
mesothoracic tergum, with Scer\GAL4pnr-MD237
mitochondrion | progressive
mitotic cell cycle
neuron | embryonic stage
ommatidium
ommatidium | somatic clone
photoreceptor cell
presumptive embryonic/larval peripheral nervous system
retina | progressive
retina | progressive | somatic clone | conditional
thorax, with Scer\GAL4pnr-MD237
trichogen cell, with Scer\GAL4pnr-MD237
wing
wing, with Scer\GAL4bbg-C96
wing, with Scer\GAL4Bx-MS1096
wing, with Scer\GAL4en.PU
wing, with Scer\GAL4nub.PK
wing, with Scer\GAL4nub.PU
wing margin
wing pouch
wing pouch, with Scer\GAL4Bx-MS1096
wing pouch, with Scer\GAL4en.PU
wing vein
wing vein, with Scer\GAL4nub.PK
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\TBL1X
15 of 15
Yes
Yes
 
Hsap\TBL1XR1
13 of 15
No
Yes
Hsap\TBL1Y
12 of 15
No
Yes
Hsap\WDR38
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Tbl1x
13 of 15
Yes
Yes
Mmus\Tbl1xr1
13 of 15
Yes
Yes
Mmus\Wdr17
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
Rnor\Tbl1xr1
11 of 13
Yes
Yes
Rnor\Tbl1x
11 of 13
Yes
Yes
Rnor\Wdr17
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\tbl1x
8 of 12
Yes
Yes
Xtro\tbl1xr1
7 of 12
No
Yes
Danio rerio (Zebrafish) (3)
Drer\tbl1x
13 of 15
Yes
Yes
Drer\tbl1xr1b
12 of 15
No
Yes
Drer\tbl1xr1a
11 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\unc-37
1 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (1)
Atha\HOS15
6 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\SIF2
10 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\hif2
7 of 12
Yes
Yes
Spom\tup12
1 of 12
No
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091905PX )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\ebi
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000040
Drosophila simulans
Dsim\GD23022
Drosophila sechellia
Dsec\GM16739
Drosophila erecta
Dere\GG24716
Drosophila yakuba
Dyak\GE16791
Drosophila ananassae
Dana\GF20651
Drosophila pseudoobscura pseudoobscura
Dpse\GA17928
Drosophila persimilis
Dper\GL18875
Drosophila willistoni
Dwil\GK24403
Drosophila virilis
Dvir\GJ17774
Drosophila mojavensis
Dmoj\GI21646
Drosophila grimshawi
Dgri\GH11222
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503CJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17009920
Glossina morsitans
Tsetse fly
Gmor\GMOY001506
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_09401
Mayetiola destructor
Hessian fly
Mdes\Mdes015676
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL003539
Anopheles darlingi
American malaria mosquito
Adar\ADAC003732
Anopheles gambiae
Malaria mosquito
Agam\AGAP007739
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ010919
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0349 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA007583
Danaus plexippus
Monarch butterfly
Dple\DPOGS204225-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL015146
Apis florea
Little honeybee
Aflo\3740288644
Apis mellifera
Western honey bee
Amel\ebi
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22598
Bombus terrestris
Buff-tailed bumblebee
Bter\15201839
Linepithema humile
Argentine ant
Lhum\LH12143
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001930
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG002461
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_06179
Tribolium castaneum
Red flour beetle
Tcas\TC003706
Pediculus humanus
Human body louse
Phum\PHUM076530
Rhodnius prolixus
Kissing bug
Rpro\RPRC005791
Cimex lectularius
Bed bug
Clec\CLEC009857
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI009292
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI004972
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03403
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0313 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR015176
Strigamia maritima
European centipede
Smar\SMAR015788
Ixodes scapularis
Black-legged tick
Isca\ISCW008840
Stegodyphus mimosarum
African social velvet spider
Smim\KFM79246.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur05g03980
Tetranychus urticae
Two-spotted spider mite
Turt\tetur05g03990
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_224492
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_253694
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_53261
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_271400
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04MW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-TbL1xr1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_005499gn
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000008885
Gallus gallus
Domestic chicken
Ggal\TBL1XR1
Gallus gallus
Domestic chicken
Ggal\TBL1X
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Evidence
    References
    ebi1-334.GMR
    model of  sensory system disease
    CEA
    (Lim et al., 2012)
    ebik16213
    model of  sensory system disease
    CEA
    (Lim et al., 2012)
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    ebik16213
    ameliorates  multiple endocrine neoplasia type 2B
    modeled by RetMEN2B.GMR
    (Read et al., 2005)
    ameliorates  multiple endocrine neoplasia type 2A
    modeled by RetMEN2A.GMR
    (Read et al., 2005)
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    TBL1X; transducin beta like 1 X-linked
    15 of 15
    300196
       
      TBL1XR1; TBL1X receptor 1
      13 of 15
      608628
      TBL1Y; transducin beta like 1 Y-linked
      12 of 15
      400033
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        ebi - Bap60
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - CG17002
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - CG2017
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - CG3008
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - CG34159
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - Cdk8
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - Cpsf6
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - CtBP
        pull down, autoradiography
        (Qi et al., 2008)
        ebi - HDAC3
        anti bait coimmunoprecipitation, western blot
        (Qi et al., 2008)
        ebi - Jra
        anti tag coimmunoprecipitation, anti tag western blot
        (Lim et al., 2012)
        ebi - Larp4B
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED10
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED11
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED14
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED15
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED16
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED17
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED18
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED20
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED23
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED25
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED27
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED30
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED4
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED6
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED7
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - MED8
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - Pa1
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - Pc
        pull down, peptide massfingerprinting
        (Strübbe et al., 2011)
        ebi - Psi
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - Ptip
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - Rbf
        anti tag coimmunoprecipitation, anti tag western blot
        (Lim et al., 2013)
        ebi - Sec23
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - Sec24AB
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - Smyd4-2
        anti tag coimmunoprecipitation, anti tag western blot
        (Thompson and Travers, 2008)
        ebi - Snr1
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - Ssdp
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - Usp10
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - boca
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - brm
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - crb
        pull down, autoradiography, two hybrid, anti tag coimmunoprecipitation, anti tag western blot
        (Nguyen et al., 2016)
        ebi - dsh
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - enc
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - ix
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - mor
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - osa
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - sina
        pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot
        (Cooper, 2007)
        ebi - sinah
        pull down, autoradiography, anti tag coimmunoprecipitation, anti tag western blot
        (Cooper, 2007)
        ebi - skd
        experimental knowledge based
        (Guruharsha et al., 2011)
        ebi - sna
        pull down, autoradiography
        (Qi et al., 2008)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        ebi
        suppressible
        crb
        (Nguyen et al., 2016)
        ebi
        enhanceable
        Dcr-2
        (Dietzl et al., 2007)
        ebi
        enhanceable
        Diap1
        (Lim et al., 2012)
        ebi
        suppressible
        Dl
        (Tsuda et al., 2002)
        ebi
        enhanceable
        Dl
        (Tsuda et al., 2002)
        ebi
        suppressible
        hep
        (Lim et al., 2012)
        ebi
        suppressible
        Jra
        (Lim et al., 2012)
        ebi
        enhanceable
        puc
        (Lim et al., 2012)
        ebi
        suppressible
        rno
        (Voas and Rebay, 2003)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        aos
        enhanceable
        ebi
        (Marygold et al., 2011, Coelho et al., 2005)
        chn
        enhanceable
        ebi
        (Tsuda et al., 2006)
        crb
        suppressible
        ebi
        (Nguyen et al., 2016)
        Dl
        enhanceable
        ebi
        (Tsuda et al., 2002)
        Dp|E2f1
        enhanceable
        ebi
        (Boulton et al., 2000)
        Egfr::tor
        suppressible
        ebi
        (Dong et al., 1999)
        Egfr
        enhanceable
        ebi
        (Dong et al., 1999)
        egr
        enhanceable
        ebi
        (Lim et al., 2012)
        N
        enhanceable
        ebi
        (Marygold et al., 2011, Tsuda et al., 2002)
        Pc
        suppressible
        ebi
        (Strübbe et al., 2011)
        Pi3K92E
        enhanceable
        ebi
        (Marygold et al., 2011, Coelho et al., 2005)
        Ret
        suppressible
        ebi
        (Read et al., 2005)
        rux
        enhanceable
        ebi
        (Dong et al., 1999)
        sd
        enhanceable
        ebi
        (Marygold et al., 2011)
        Ser
        enhanceable
        ebi
        (Tsuda et al., 2002)
        sev|Sos
        enhanceable
        ebi
        (Dong et al., 1999)
        sno
        enhanceable
        ebi
        (Tsuda et al., 2002)
        External Data
        Subunit Structure (UniProtKB)
        Component of some E3 complex at least composed of sina, ebi and phyl, required for the degradation of ttk. Probably forms a E3 complex with sno, required for the degradation of some component of the Su(H) repressor complex. Interacts with sno and Su(H) and Smr.
        (UniProt, Q95RJ9 )
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Positive Regulators of Notch Signaling Pathway -
        The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
        Metabolic Pathways
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map

        2-0.7

        Cytogenetic map
        21C2-21C2
        Sequence location
        2L:415,067..418,536 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        21C2-21C2
        Limits computationally determined from genome sequence between P{lacW}RpI135k16513&P{lacW}ebik16213 and P{lacW}l(2)k09610k09610&P{lacW}Sk09538a
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        21B-21D
        (Dong, 1999.4.27)
        21C2-21C3
        (determined by in situ hybridisation)
        (Spradling et al., 1999)
        21C2-21C3
        (BDGP Project Members, 1994-1999)
        Experimentally Determined Recombination Data
        Location

        2-0.7

        (FlyBase, 2016)

        2-0.0

        (Comeron et al., 2012)

        2-

        (Coelho et al., 2005)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (25)
        Genomic Clones (15)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (120)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequences
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: ebi CG4063

        (Guan, 1999.11)
        Source for database merge of

        Source for merge of: ebi l(2)k16213

        (Dong et al., 1999)

        Source for merge of: ebi E-2g

        (Marygold et al., 2011)
        Additional comments
        Other Comments

        Phenotypes in trans with ebiP7 and genetic interactions order ebi mutations into the following allelic series (strongest to weakest): ebi2g2, ebiE90, ebiCC3, ebi2g1, ebiCCS-8, ebiE4, ebiΔ1, ebiW418X, ebik16213, ebiWKS-24, ebiP7.

        (Marygold et al., 2011)

        Identification: as a complementation group whose members dominantly modify the small wing phenotype caused by expression of Pi3K92ED954A.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096. 2 alleles of E-2g have been recovered.

        (Coelho et al., 2005)

        sno and ebi play a key role in linking the activation of the Egfr pathway to the expression of Dl in R cells by inactivation of Su(H)-mediated repression.

        (Tsuda et al., 2002)

        ebi has two distinct functions; it promotes the degradation of a repressor of neuronal differentiation ttk88, and has a second independant function that limits S phase entry.

        (Boulton et al., 2000)

        ebi is required for Egfr but not tor-receptor-dependent gene expression.

        (Dong et al., 1999)

        May function in MAPK signaling pathways.

        (Tsuda et al., 1997)

        Mutations do not show genetic interactions with tor or sev mutations.

        (Tsuda et al., 1997)
        Origin and Etymology
        Discoverer
        Etymology

        'ebi' means 'shrimp' in Japanese.

        (Dong et al., 1999)
        Identification
        External Crossreferences and Linkouts ( 42 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
        Linkouts
        ApoDroso - Functional genomic database for photoreceptor development, survival and function
        DroID - A comprehensive database of gene and protein interactions.
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (11)
        Reported As
        Symbol Synonym
        CG4063
        (Kondo et al., 2017, Hosono et al., 2015, Roberts et al., 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Grieder et al., 2007, Brandt, 2006)
        E-2f
        (Coelho et al., 2005)
        E-2g
        Ebi
        (Nguyen et al., 2016, Fiedler et al., 2015, Mannervik, 2014, Roberts et al., 2012, Strübbe et al., 2011, Thompson and Travers, 2008, Cooper, 2007, Tsuda et al., 2006, Goode et al., 2005, Hall et al., 2005, Sundaram, 2005, Tsuda et al., 2005, Voas and Rebay, 2004, Dong, 1999.4.27)
        FBXW4
        (Roberts et al., 2012)
        Tb11
        (Spradling et al., 1999)
        Tbl1
        (Spradling et al., 1999)
        ebi
        (Tsui et al., 2018, Houtz et al., 2017, Kondo et al., 2017, Lim and Tsuda, 2016, Nguyen et al., 2016, Sarov et al., 2016, Dietz et al., 2015, Lim et al., 2015, Toret et al., 2014, Das et al., 2013, Debruhl et al., 2013, Handler et al., 2013, Kockmann et al., 2013, Lim et al., 2013, Wang et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Lim et al., 2012, Murray et al., 2012, Roberts et al., 2012, Friedman et al., 2011, Marygold et al., 2011, Strübbe et al., 2011, Toku et al., 2011, Inomata et al., 2008, Qi et al., 2008, Zhao et al., 2008, Dietzl et al., 2007, Kaplow et al., 2007, Nagaraj and Banerjee, 2007, Brandt, 2006, Boulton et al., 2000)
        l(2)k16213
        Name Synonyms
        Ebi
        (Lim and Thiery, 2012, Cooper, 2007)
        Enhancer on chromosome 2-complementation group 2g
        (Coelho et al., 2005)
        beta transducin-like 1 protein
        (Spradling et al., 1999)
        ebi
        (Dong et al., 1999)
        Secondary FlyBase IDs
        • FBgn0023444
        • FBgn0021820
        • FBgn0085068
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (106)