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Gene Dmel\bru-3

General Information
Symbol	Dmel\bru-3	Species	D. melanogaster
Name	bruno-3	Annotation symbol	CG43744
Feature type	protein_coding_gene	FlyBase ID	FBgn0264001
Gene Model Status	Current	Stock availability	22 publicly available
Also Known As	CG12478, CG10046, CG34243, Dm Bru-3
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	70C1-70C2	Sequence location	3L:13,515,293..13,796,500 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene bruno-3 is referred to in FlyBase by the symbol Dmel\bru-3 (CG43744, FBgn0264001). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA binding; protein homodimerization activity. Based on sequence similarity, it is predicted to be involved in the biological process: negative regulation of translation. 22 alleles are reported. The phenotype of these alleles is annotated with: eye. It has 15 annotated transcripts and 15 annotated polypeptides. Protein features are: Nucleotide-binding, alpha-beta plait; RNA recognition motif domain. Gene sequence location is 3L:13515293..13796500.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Alleles bru-3[MI03550]
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 70C1-70C2   Left limit from in situ hybridisation (FBrf0067338) Right limit from non-inclusion within Df(3L)fz-GF3b (FBrf0067338)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 70C1-70C2   (determined by in situ hybridisation)   (Spradling et al., 1999) 70C1-70C2     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\bru-3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Gene model reviewed during 5.46 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated The merge of bru-3 and CG34243 resulted in the loss of the CDS previously associated with CG34243. Gene merge based on EST/cDNA and RNA-seq junction and expression data.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) bru-3-RA FBtr0330343  NM_168557   2587   422 bru-3-RB FBtr0330347  NM_079335   2410   363 bru-3-RC FBtr0330349  NM_001202161   2509   396 bru-3-RD FBtr0330344  NM_001202162   2467   382 bru-3-RE FBtr0330352  NM_001202163   5007   408 bru-3-RF FBtr0330346  NM_001202164   2332   337 bru-3-RG FBtr0330353  NM_001202165   4953   390 bru-3-RH FBtr0330351  NM_001202166   10570   416 bru-3-RI FBtr0330345  NM_001202167   3865   384 bru-3-RJ FBtr0330354  NM_001202168   2786   49 bru-3-RK FBtr0330348  NM_001202169   2314   331 bru-3-RL FBtr0330350  NM_001202170   2356   345 bru-3-RM FBtr0330355  NM_001202171   6771   370 bru-3-RN FBtr0330356  NM_001274878   2929   403 bru-3-RO FBtr0330357  NM_001274879   2665   403
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein bru-3-PA   FBpp0303375   45.1   422   7.91     NP_729923   AAF49798 bru-3-PB   FBpp0303379   38.5   363   8.33     NP_524059   AAF49799 bru-3-PC   FBpp0303381   42.4   396   7.91     NP_001189090   ADV37526 bru-3-PD   FBpp0303376   40.9   382   7.62     NP_001189091   ADV37527 bru-3-PE   FBpp0303384   43.6   408   7.62     NP_001189092   ADV37528 bru-3-PF   FBpp0303378   35.7   337   8.33     NP_001189093   ADV37529 bru-3-PG   FBpp0303385   41.7   390   7.31     NP_001189094   ADV37530 bru-3-PH   FBpp0303383   44.4   416   7.31     NP_001189095   ADV37531 bru-3-PI   FBpp0303377   41.0   384   7.05     NP_001189096   ADV37532 bru-3-PJ   FBpp0303386   5.5   49   9.45     NP_001189097   ADV37533 bru-3-PK   FBpp0303380   35.0   331   8.62     NP_001189098   ADV37534 bru-3-PL   FBpp0303382   36.6   345   8.01     NP_001189099   ADV37535 bru-3-PM   FBpp0303387   39.2   370   7.58     NP_001189100   ADV37536 bru-3-PN   FBpp0303388   43.1   403   8.48     NP_001261807   AGB94500 bru-3-PO   FBpp0303389   43.1   403   8.48     NP_001261808   AGB94501
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Nucleotide-binding, alpha-beta plait (IPR012677) RNA recognition motif domain (IPR000504)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141456   AA141593   AA141714   AA141885   AA390283   AA695925   AA696434   AA697719   AA951398   AE014296 AAF49798   AE014296 AAF49799   AE014296 ADV37526   AE014296 ADV37527   AE014296 ADV37528   AE014296 ADV37529   AE014296 ADV37530   AE014296 ADV37531   AE014296 ADV37532   AE014296 ADV37533   AE014296 ADV37534   AE014296 ADV37535   AE014296 ADV37536   AE014296 AGB94500   AE014296 AGB94501   AI533463   AQ026210   AW943103   AY060398 AAL25437   BE977148   BF489526   BG632190   BG633059   BG637093   BG639587   BG639593   BI142092   BI234781   BI241908   BI355868   BI358319   BI367893   BI368978   BI563517   BI565420   BI566503   BI570033   BI571671   BI571679   BI582636   BI585783   BI586507   BI588633   BI589043   BI589697   BI604852   BI605922   BI613124   BI614927   BI615716   BI617811   BI626477   BI634403   BI638345   BI639929   BT056287   BT126368   CA807100   CO177549   CO178669   CO179410   CO179610   CO190268   CO262294   CO265804   CO266528   CO267012   CO268279   CO268775   CO268945   CO269723   CO269956   CO270891   CO271243   CO272166   CO272320   CO273005   CO273402   CO274896   CO275886   CO278056   CO279733   CO280947   CO281268   CO281528   CO281956   CO283731   CO283747   CO284589   CO284981   CO285494   CO286248   CO286446   CO288445   CO291601   CO291625   CO292457   CO294915   CO295160   CO295477   CO296503   CO297858   CO299908   CO300363   CO300848   CO302803   CO303448   CO304688   CO304728   CO308750   CO309979   CO310946   CO311288   CO312884   CO314047   CO315420   CO315692   CO316193   CO316223   CO316786   CO318996   CO320331   CO320679   CO321061   CO321607   CO325225   CO325983   CO326143   CO327053   CO328882   CO329578   CO329771   CO331743   CO331749   CO332242   CO333970   CO336331   CO339030   CO339033   CO339724   CO340147   CO340901   CO341425   CO341811   CO342049   CO343565   CO344320   CO344644   CZ466236   CZ476973   CZ487833   CZ487834   CZ487857   CZ488044   CZ488352   CZ490074   EC064738   EC066356   EC067381   EC069448   EC082864   EC085399   EC086393   EC087515   EC089259   EC199904   EC202323   EC202905   EC203231   EC206437   EC209027   EC226885   EC236545   EC238974   EC244621   EC250596   GO271028
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	A8JNS5 Q9VU91 Q9VU90
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) midline primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) visceral muscle primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   dorsal sensory complex proper primordium   (Fisher et al., 2012) Comment:reported as dorsal/lateral sensory complexes embryonic antennal sense organ   (Fisher et al., 2012) embryonic brain   (Fisher et al., 2012) embryonic foregut sensory structure   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic labial sensory complex   (Fisher et al., 2012) embryonic maxillary sensory complex   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
protein complex inferred from direct assay (Delaunay et al., 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    46 central nervous system, pupae P8    99 head, virgin 1-day female    26 head, virgin 4-day female    26 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    21 head, mated 20-day female    11 head, mated 1-day male    33 head, mated 4-day male    40 head, mated 20-day male    25 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3  (46) central nervous system, pupae P8  (99) head, virgin 1-day female  26 head, virgin 4-day female  26 head, virgin 20-day female    10 head, mated 1-day female  26 head, mated 4-day female    21 head, mated 20-day female    11 head, mated 1-day male  (33) head, mated 4-day male  (40) head, mated 20-day male    25 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    46 central nervous system, pupae P8    99 head, virgin 1-day female    26 head, virgin 4-day female    26 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    21 head, mated 20-day female    11 head, mated 1-day male    33 head, mated 4-day male    40 head, mated 20-day male    25 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    46 central nervous system, pupae P8    99 head, virgin 1-day female    26 head, virgin 4-day female    26 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    21 head, mated 20-day female    11 head, mated 1-day male    33 head, mated 4-day male    40 head, mated 20-day male    25 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    46 central nervous system, pupae P8    99 head, virgin 1-day female    26 head, virgin 4-day female    26 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    21 head, mated 20-day female    11 head, mated 1-day male    33 head, mated 4-day male    40 head, mated 20-day male    25 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3  (46) central nervous system, pupae P8  (99) head, virgin 1-day female  26 head, virgin 4-day female  26 head, virgin 20-day female    10 head, mated 1-day female  26 head, mated 4-day female    21 head, mated 20-day female    11 head, mated 1-day male  (33) head, mated 4-day male  (40) head, mated 20-day male    25 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    46 central nervous system, pupae P8    99 head, virgin 1-day female    26 head, virgin 4-day female    26 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    21 head, mated 20-day female    11 head, mated 1-day male    33 head, mated 4-day male    40 head, mated 20-day male    25 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    46 central nervous system, pupae P8    99 head, virgin 1-day female    26 head, virgin 4-day female    26 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    21 head, mated 20-day female    11 head, mated 1-day male    33 head, mated 4-day male    40 head, mated 20-day male    25 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    1 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    5 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    6 embryo 22-24hr    9 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    5 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    6 embryo 22-24hr    9 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    5 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    6 embryo 22-24hr    9 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    5 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    6 embryo 22-24hr    9 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    5 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    6 embryo 22-24hr    9 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    5 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    6 embryo 22-24hr    9 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    5 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    6 embryo 22-24hr    9 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    5 embryo 12-14hr    8 embryo 14-16hr    6 embryo 16-18hr    9 embryo 18-20hr    8 embryo 20-22hr    6 embryo 22-24hr    9 larva L1    5 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    2 white prepupae new    2 white prepupae 12hr    3 white prepupae 24hr    4 pupae 2d postWPP    5 pupae 3d postWPP    3 pupae 4d postWPP    1 adult male 01day    1 adult male 05day    2 adult male 30day    2 adult female 01day    1 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    13 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    13 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    13 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    13 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    13 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    13 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    13 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    13 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    4 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_29 mE1_20_mRNA_expression_cluster_08
External Data & Images
Linkouts	BDGP expression data • LD31834
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4Act.PU bru-3EY08487 lethal (with Df(3L)Exel6119) bru-305871 lethal | recessive bru-305871 partially lethal - majority die (with Df(3L)Exel6119) bru-3EY08487 viable, with Scer\GAL4pnr-MD237 bru-3GD12651 viable | reduced (with bru-305871) bru-3EY08487 viable | reduced (with bru-3EY08487) bru-305871 Other Phenotypes Allele visible bru-3EY08487 Phenotype manifest in Allele eye bru-3EY08487
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of bru-3 Class Mutagen Stocks Known lesion bru-305871 P-element activity 1 Yes bru-3BG01691 1 -- bru-3c00149 piggyBac transposase 1 -- bru-3d09837 P-element activity 1 -- bru-3EY08487 1 -- bru-3f01923 1 -- bru-3f05208 piggyBac transposase 1 -- bru-3f05228 piggyBac transposase 1 -- bru-3f05463 piggyBac transposase 1 -- bru-3f05729 piggyBac transposase 1 -- bru-3f07261 piggyBac transposase 1 -- bru-3MB04010 1 -- bru-3MB07091 1 -- bru-3MB11495 1 -- bru-3MI02379 1 -- bru-3MI03550 1 -- bru-3MI03997 1 -- bru-3MI05016 1 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of bru-3 Class Mutagen Stocks Known lesion bru-3GD12651 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes bru-3HMS02702 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes bru-3KK111663 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes bru-3KK114804 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes bru-3MtnA.T:Zzzz\His6 in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(3L)fz-CAL5 (BDGP Project Members, 1994-1999, Spradling et al., 1999) Df(3L)fz-GF3b (BDGP Project Members, 1994-1999, Spradling et al., 1999) Df(3L)fz-GS1a (BDGP Project Members, 1994-1999, Spradling et al., 1999)
Disrupted in	Df(3L)Exel6119 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12651} NA P{KK111663} NA P{TRiP.HMS02702} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}bru-305871 No insertion of mobile activating element P{EPgy2}bru-3EY08487 NA P{XP}bru-3d09837 NA miscellaneous insertions Mi{MIC}bru-3MI02379 NA Mi{MIC}bru-3MI03550 NA Mi{MIC}bru-3MI03997 NA Mi{MIC}bru-3MI05016 NA P{GT1}bru-3BG01691 NA PBac{PB}bru-3c00149 NA PBac{WH}bru-3f01923 NA PBac{WH}bru-3f05208 NA PBac{WH}bru-3f05228 NA PBac{WH}bru-3f05463 NA PBac{WH}bru-3f05729 NA PBac{WH}bru-3f07261 NA insertion of enhancer trap binary system Mi{ET1}bru-3MB04010 No Mi{ET1}bru-3MB07091 No Mi{ET1}bru-3MB11495 No
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
protein homodimerization activity inferred from direct assay (Delaunay et al., 2004) RNA binding inferred from direct assay (Delaunay et al., 2004)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
protein complex inferred from direct assay (Delaunay et al., 2004)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
mRNA binding inferred from sequence or structural similarity (Lasko, 2000) inferred from sequence or structural similarity with EMBL:U16800 (FlyBase, 1992-) nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
negative regulation of translation inferred from sequence alignment with aret (Delaunay et al., 2004)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene (FlyBase Genome Annotators, 2007)
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
bru-3 - ATPsyn-γ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - CG10306 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - CG12264 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - CG5009 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - CG7033 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - CG7766 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) bru-3 - CG8475 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) bru-3 - CG9911 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - Chc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - Faa anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) bru-3 - Hsp70Aa anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - LanB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - Nup358 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - PhKγ experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) bru-3 - Rop anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - RpS19a anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - Rpt2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - Rpt5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - Sec61α anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - TER94 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - Tcp-1ζ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - Tom20 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - abs anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - coro experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) bru-3 - eIF6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - enc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - l(1)G0230 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpL1 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) bru-3 - mRpL15 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpL23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpL3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpL39 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpL41 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpL44 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpL51 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpL52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpL53 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpL9 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - mRpS30 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - pit anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - polo anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - sqh anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) bru-3 - γTub37C experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions bru-3 allele Gene References
External Data
Linkouts
Orthologs
OrthoDB Orthologs (60) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG644M5S Dipteran inclusive ortholog search EOG6CG0J0 Insect inclusive ortholog search EOG63TXR8 Arthropod inclusive ortholog search EOG6FXPRB Metazoa inclusive ortholog search EOG6TBH66, EOG6TJ7QM
Orthologs in Drosophila Species (EOG644M5S)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\bru-3     Drosophila simulans   Dsim\bru-3 Y   Drosophila sechellia   Dsec\bru-3 Y   Drosophila erecta   Dere\bru-3 Y   Drosophila yakuba   Dyak\bru-3 Y   Drosophila ananassae   Dana\bru-3 Y   Drosophila pseudoobscura pseudoobscura   Dpse\bru-3 Y   Drosophila persimilis   Dper\bru-3 Y   Drosophila willistoni   Dwil\bru-3 Y   Drosophila virilis   Dvir\bru-3 Y   Drosophila mojavensis   Dmoj\bru-3 Y   Drosophila grimshawi   Dgri\bru-3 Y
Orthologs in non-Drosophila Dipterans (EOG6CG0J0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL003979   Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014033   Anopheles gambiae Malaria mosquito  Agam\AGAP004950   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ010770   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ010768
Orthologs in non-Dipteran Insects (EOG63TXR8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49723 Y Apis mellifera Western honey bee  Amel\GB49722 Y Apis mellifera Western honey bee  Amel\GB55730 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007495 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG011447 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007494 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH23912 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH19063 Y Atta cephalotes Leafcutter ant  Acep\ACEP19362 Y Atta cephalotes Leafcutter ant  Acep\ACEP19366 Y Atta cephalotes Leafcutter ant  Acep\ACEP19365 Y Atta cephalotes Leafcutter ant  Acep\ACEP20982 Y Atta cephalotes Leafcutter ant  Acep\ACEP20981 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO21581 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO15812 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14351 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15266 Y Linepithema humile Argentine ant  Lhum\LH21841 Y Linepithema humile Argentine ant  Lhum\LH17161 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB21336 Y Solenopsis invicta Red fire ant  Sinv\SINV12096 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI55194 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI083518 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001379 Y Bombyx mori Silkmoth  Bmor\BGIBMGA010431 Y Bombyx mori Silkmoth  Bmor\BGIBMGA005893 Y Bombyx mori Silkmoth  Bmor\BGIBMGA010430 Y Pediculus humanus Human body louse  Phum\PHUM315130 Y Pediculus humanus Human body louse  Phum\PHUM533900 Y Pediculus humanus Human body louse  Phum\PHUM533590 Y Tribolium castaneum Red flour beetle  Tcas\TC001521 Y Tribolium castaneum Red flour beetle  Tcas\TC001522 Y Tribolium castaneum Red flour beetle  Tcas\TC012080 Y
Orthologs in non-Insect Arthropods (EOG6FXPRB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\COII Y Daphnia pulex Water flea  Dpux\PB2 Y Daphnia pulex Water flea  Dpux\NEMF Y Daphnia pulex Water flea  Dpux\JGI_V11_227924 Y Ixodes scapularis Deer tick  Isca\ISCW008885 Y Ixodes scapularis Deer tick  Isca\ISCW010799 Y Ixodes scapularis Deer tick  Isca\ISCW019058 Y Ixodes scapularis Deer tick  Isca\ISCW005543 Y
Orthologs in non-Arthropod Metazoa (EOG6TBH66, EOG6TJ7QM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Xenopus tropicalis Western clawed frog  Xtro\celf3   Mus musculus House mouse  Mmus\Celf6
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\bru-3 Drosophila sechellia Dsec\bru-3 Drosophila erecta Dere\bru-3 Drosophila yakuba Dyak\bru-3 Drosophila ananassae Dana\bru-3 Drosophila pseudoobscura pseudoobscura Dpse\bru-3 Drosophila persimilis Dper\bru-3 Drosophila willistoni Dwil\bru-3 Drosophila virilis Dvir\bru-3 Drosophila mojavensis Dmoj\bru-3 Drosophila grimshawi Dgri\bru-3
Stocks & Reagents
Stocks Listed in FlyBase ( 22 )
Bloomington	27747 y1; Mi{ET1}bru-3MB07091
Harvard	- P{XP}bru-3d09837 - PBac{PB}bru-3c00149 - PBac{WH}bru-3f01923 - PBac{WH}bru-3f05208 - PBac{WH}bru-3f05228 - PBac{WH}bru-3f05463 - PBac{WH}bru-3f05729 - PBac{WH}bru-3f07261
VDRC	v102442 P{KK111663}VIE-260B v35526 w1118; P{GD12651}v35526 v35525 w1118; P{GD12651}v35525/CyO
	More stocks available...
Genomic Clones ( 4 )
	BACR03I13 BACR17E13 BACR17J20 BACR29O22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 161 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD31834
Other clones	DPiM_LD31834 FI14817 MIP02464
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT25571 GH04903 GH19363 GM04777 GM07672 GM32653 LD09131 RE30537 RE39109 RE45055 RE53763 RE55048 RH02270 RH03168 RH06941 RH06949 RH20912 RH25441 RH26465 RH30020 RH30716 RH34163 RH36065 RH38452 RH42210 RH44427 RH45444 RH48255 RH63191 RH67433 RH70341 RH71937 SD03009 SD03038 SD05284 SD14952 SD16092 SD20610 SD22651
Other clones	31652 53420 54006 67902 98103 98315 100347 102718 117672 123567 158015 158530 161694 174449 182650 191212 bs60c09 CK01.29D.G11 CK01.58D.B10 CK01.142C.E8 CK01.170D.C3 CK01771 CK02256 CK02684 CK02693 EK047639 EK053803 EK057316 EK059327 EK060011 EK062229 EK062839 EK068527 EK084963 EK090809 EK092643 EK093145 EK095703 EK097007 EK100937 EK102527 EK106046 EK106728 EK109205 EK110833 EK115852 EK119043 EK128624 EK135752 EK143704 EK146664 EK148339 EK155537 EK156329 EK157360 EK159163 EK164205 EK164230 EK166349 EK167802 EK169108 EK170755 EK176904 EK178032 EK179526 EK184927 EK186020 EK195856 EK197806 EK197836 EK200229 EK207953 EK208757 EK209943 EK213836 EK220109 EK223028 EK224049 EK230464 EK234764 EK241457 EK245728 EK245947 EK259242 EK261348 EK262215 EK263921 EK264004 EK265849 EK275730 EK284052 EK286964 EK290234 EK291606 EK293141 EK293829 EK301608 EK301618 EK302908 EK308507 EN12324 EP00644 EP00647 EP03024 EP03318 EP05631 EP10112 EP11538 EP12425 EP13216 EP13712 EP17250 EP19044 EP19647 EP22363 ESG016b.E21_B12 FB59-CG34243-RA HL03040
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: bru-3 CG12478 (Good et al., 2000)
Source for database merge of	Source for merge of: bru-3 CG10046 Annotations CG12478, CG10046 merged as CG12478 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Source for merge of: bru-3 l(3)05871 (Beam and Moberg, 2010)
Additional comments	"gfr" mutant alleles do not behave as simple loss-of-function mutations: they are lethal in trans to each other, yet completely viable and without obvious phenotypes in trans to all available deficiencies and lethal lesions mapping to the 70A-70B chromosomal region (to which the "gfr" complementation group maps). Evidence suggests that "gfr" alleles behave as gain-of-function lesions and that overexpression of "bru-3" is central to the "gfr" mutant phenotype. (Beam and Moberg, 2010)
Other Comments
	New annotation (CG34243) in release 5.2 of the genome annotation. (FlyBase Genome Annotators, 2007)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079335 NM_168557 NM_001202161 NM_001202162 NM_001202163 NM_001202164 NM_001202165 NM_001202166 NM_001202167 NM_001202168 NM_001202169 NM_001202170 NM_001202171 NM_001274878 NM_001274879 RefSeq (Proteins) • NP_524059 NP_729923 NP_001189090 NP_001189091 NP_001189092 NP_001189093 NP_001189094 NP_001189095 NP_001189096 NP_001189097 NP_001189098 NP_001189099 NP_001189100 NP_001261807 NP_001261808 DDBJ / EMBL / GenBank • AA951398 AAF49798 AAF49799 AAL25437 ABW08537 ADV37526 ADV37527 ADV37528 ADV37529 ADV37530 ADV37531 ADV37532 ADV37533 ADV37534 ADV37535 ADV37536 AGB94500 AGB94501 AQ026210 AW943103 AY060398 CZ466236 CZ476973 CZ487833 CZ487834 CZ487857 CZ488044 CZ488352 CZ490074 Entrez Gene - A searchable database of RefSeq genes. • 39527 5740341 UniProtKB/TrEMBL • A8JNS5 Q9VU90 Q9VU91
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Nucleotide-binding, alpha-beta plait (IPR012677) RNA recognition motif domain (IPR000504) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD31834
Linkouts
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	bru-3 (Bellen et al., 2011, Hartmann et al., 2011, Ellis and Carney, 2011, Beam and Moberg, 2010, Smibert et al., 2012) Bru-3 (Delaunay et al., 2004, Kandul and Noor, 2009) bruno-3 (Dalton et al., 2010) CG10046 (Alias and Clark, 2007, Robertson, 2001.5.6, Good, 2000.3.24, ) cg10046 (Lasko, 2000) CG12478 (Ellis and Carney, 2011, Bandyopadhyay et al., 2006, Robertson, 2001.5.6, Keleman et al., 2009.8.5) cg12478 (Lasko, 2000) CG34243 (Keleman et al., 2009.8.5, FlyBase Genome Annotators, 2007, Remolina et al., 2012) CG43744 (Ni et al., 2010.12.1) Dm Bru-3 (Horb and Horb, 2010, Good et al., 2000) l(3)05871
Name Synonym	bruno-3 (Beam and Moberg, 2010, Good et al., 2000, Burnette et al., 2005, Stofanko et al., 2008, Kandul and Noor, 2009, ) bruno3 (Vicente-Crespo et al., 2008) lethal (3) 05871
Secondary FlyBase IDs
	FBgn0010879 FBgn0036378 FBgn0036379 FBgn0085272 FBgn0262350
References ( 53 )
Generate a list of	All references relating to this gene ( 53 )
List References by type	Research paper  ( 19 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 20 ) FlyBase analysis  ( 4 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 4 )
	Smibert et al., 2012, Cell Rep. 1(3): 277--289 Global Patterns of Tissue-Specific Alternative Polyadenylation in Drosophila. [FBrf0218523] Bellen et al., 2011, Genetics 188(3): 731--743 The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229] Ellis and Carney, 2011, Genetics 187(1): 157--169 Socially-Responsive Gene Expression in Male Drosophila melanogaster Is Influenced by the Sex of the Interacting Partner. [FBrf0212780] Hartmann et al., 2011, RNA 17(3): 453--468 Distinct regulatory programs establish widespread sex-specific alternative splicing in Drosophila melanogaster. [FBrf0212988] Show all research papers ...