FlyBase Gene Report: Dmel\bru3

General Information

Symbol

Dmel\bru3

Species

D. melanogaster

Name

bruno 3

Annotation Symbol

CG43744

Feature Type

protein_coding_gene

FlyBase ID

FBgn0264001

Gene Model Status

Current

Stock Availability

66 publicly available

Gene Summary

bruno 3 (bru3) encodes an RNA binding protein that binds to the EDEN element and mediates maternal mRNA translational repression. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

bru-3, Dm Bru-3

Key Links

Genomic Location

Cytogenetic map

70B4-70C4

Sequence location

3L:13,521,907..13,803,400 [-]

Recombination map

3-41

RefSeq locus

NT_037436 REGION:13521907..13803400

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein homodimerization activity

inferred from direct assay

(Delaunay et al., 2004)

enables RNA binding

inferred from direct assay

(Delaunay et al., 2004)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables mRNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000609546

(GO Reference Genome Project, 2011-)

enables RNA binding

inferred from electronic annotation with InterPro:IPR000504

(InterPro Project Members, 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in mRNA splice site recognition

inferred from biological aspect of ancestor with PANTHER:PTN002769006

(GO Reference Genome Project, 2011-)

involved_in negative regulation of translation

inferred from sequence alignment with FLYBASE:bru1; FB:FBgn0000114

(Delaunay et al., 2004)

involved_in regulation of alternative mRNA splicing, via spliceosome

inferred from biological aspect of ancestor with PANTHER:PTN002769006

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of protein-containing complex

inferred from direct assay

(Delaunay et al., 2004)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000609546

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000609546

(GO Reference Genome Project, 2011-)

part_of ribonucleoprotein complex

inferred from biological aspect of ancestor with PANTHER:PTN002768817

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

bruno 3 (bru3) encodes an RNA binding protein that binds to the EDEN element and mediates maternal mRNA translational repression. [Date last reviewed: 2019-09-19]

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\bru3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A6M3Q6L8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.46

Stop-codon suppression (UAA) postulated; FBrf0243886.

Gene model reviewed during 6.32

The merge of bru-3 and CG34243 resulted in the loss of the CDS previously associated with CG34243.

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

bru3-RA

FBtr0330343

NM_168557

2587

422

bru3-RB

FBtr0330347

NM_079335

2410

363

bru3-RC

FBtr0330349

NM_001202161

2509

396

bru3-RD

FBtr0330344

NM_001202162

2467

382

bru3-RE

FBtr0330352

NM_001202163

5007

408

bru3-RF

FBtr0330346

NM_001202164

2332

337

bru3-RG

FBtr0330353

NM_001202165

4953

390

bru3-RH

FBtr0330351

NM_001202166

10856

416

bru3-RI

FBtr0330345

NM_001202167

3865

384

FBtr0330354

2786

FBtr0330348

2314

331

bru3-RL

FBtr0330350

NM_001202170

2356

345

bru3-RM

FBtr0330355

NM_001202171

6771

370

bru3-RN

FBtr0330356

NM_001274878

2929

403

bru3-RO

FBtr0330357

NM_001274879

2665

403

bru3-RP

FBtr0344819

NM_001382115

2545

408

bru3-RQ

FBtr0479782

NM_001382116

2545

552

bru3-RR

FBtr0479783

NM_001382117

2530

435

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

bru3-PA

FBpp0303375

45.1

422

7.91

FBpp0303379

38.5

363

8.33

FBpp0303381

42.4

396

7.91

FBpp0303376

40.9

382

7.62

FBpp0303384

43.6

408

7.62

FBpp0303378

35.7

337

8.33

FBpp0303385

41.7

390

7.31

FBpp0303383

44.4

416

7.31

FBpp0303377

41.0

384

7.05

FBpp0303386

5.5

9.45

FBpp0303380

35.0

331

8.62

FBpp0303382

36.6

345

8.01

FBpp0303387

39.2

370

7.58

FBpp0303388

43.1

403

8.48

FBpp0303389

43.1

403

8.48

FBpp0311136

43.6

408

7.62

M9MRU5

QJC18618

bru3-PQ

FBpp0428115

59.4

552

8.29

QJC18619

bru3-PR

FBpp0428116

46.2

435

7.36

QJC18620

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

408 aa isoforms: bru3-PE, bru3-PP

403 aa isoforms: bru3-PN, bru3-PO

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bru3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.66

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

midline primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of protein-containing complex

inferred from direct assay

(Delaunay et al., 2004)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\bru3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\bru3

View exonic expression by tissue for Dmel\bru3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0264001

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39527/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264001

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264001

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12478

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 28 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of bru3

Transgenic constructs containing regulatory region of bru3

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene not disrupted in

Df(3L)fz-CAL5

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(3L)fz-GF3b

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(3L)fz-GS1a

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Gene disrupted in

Df(3L)Exel6119

(Ryder, 2004.4.26)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act.PU

bru3^EY08487

lethal, with Scer\GAL4^nub-AC-62

bru3^HMS02702

lethal (with Df(3L)Exel6119)

bru3⁰⁵⁸⁷¹

lethal | recessive

bru3⁰⁵⁸⁷¹

partially lethal (with bru3⁰⁵⁸⁷¹)

bru3^EY08487

partially lethal (with bru3^EY08487)

bru3⁰⁵⁸⁷¹

partially lethal - majority die (with Df(3L)Exel6119)

bru3^EY08487

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

bru3^GD12651

viable, with Scer\GAL4^Mef2.PR

bru3^GD12651

viable, with Scer\GAL4^pnr-MD237

bru3^GD12651

viable, with Scer\GAL4^tin.CΔ4

bru3^GD12651

Sterility

Allele

female semi-fertile, with Scer\GAL4^VP16.otu

bru3^HMC03283

Other Phenotypes

Allele

short lived | female limited, with Scer\GAL4^dome-PG14

visible

bru3^EY08487

visible, with Scer\GAL4^nub-AC-62

bru3^HMC03283

Phenotype manifest in

Allele

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^Pxn.PS

bru3^EY08487

eye

bru3^EY08487

ovariole

bru3^MB07091

ovariole, with Scer\GAL4^tj.PU

bru3^KK111663

wing, with Scer\GAL4^nub-AC-62

bru3^HMC03283

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (35)

Hsap\CELF5

13 of 14

Yes

Hsap\CELF3

12 of 14

Yes

Hsap\CELF6

12 of 14

Yes

Hsap\CELF4

11 of 14

Yes

6 of 14

6 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (33)

Rnor\Celf5

13 of 14

Yes

Rnor\Celf3

12 of 14

Yes

Rnor\Celf6

12 of 14

Yes

Rnor\Celf4

11 of 14

Yes

6 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (31)

Mmus\Celf5

14 of 14

Yes

Mmus\Celf6

13 of 14

Yes

Mmus\Celf3

12 of 14

Yes

Mmus\Celf4

12 of 14

Yes

6 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (19)

Xtro\celf5

11 of 13

Yes

Xtro\celf4

9 of 13

Yes

Xtro\celf3

6 of 13

Yes

5 of 13

5 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (36)

Drer\celf5a

14 of 14

Yes

Drer\celf3a

12 of 14

Yes

Drer\celf3b

12 of 14

Yes

Drer\celf4

11 of 14

Yes

Drer\celf5b

11 of 14

Yes

Drer\celf6

11 of 14

Yes

5 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (17)

Cele\unc-75

11 of 14

Yes

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (23)

Agam\AgaP_AGAP004950

6 of 12

Yes

Agam\AgaP_AGAP009477

6 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (45)

Atha\AT1G03457

12 of 13

Yes

Atha\RBP-DR1

12 of 13

Yes

Atha\FCA

7 of 13

Yes

Atha\AT2G47310

5 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (12)

Scer\GBP2

1 of 13

Yes

Scer\HRP1

1 of 13

Yes

Scer\HSH49

1 of 13

Yes

Scer\MUD1

1 of 13

Yes

Scer\NGR1

1 of 13

Yes

Scer\NOP15

1 of 13

Yes

Scer\PAB1

1 of 13

Yes

Scer\RIE1

1 of 13

Yes

Scer\TIF35

1 of 13

Yes

Scer\WHI3

1 of 13

Yes

Scer\WHI4

1 of 13

Yes

Scer\YRA1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (14)

Spom\SPBC660.15

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:bru3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (28)

10 of 13

9 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Spx

2 of 13

ssx

2 of 13

Sxl

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

fne

1 of 13

Hrb98DE

1 of 13

LS2

1 of 13

maca

1 of 13

Pof

1 of 13

Rbp9

1 of 13

Rnp4F

1 of 13

snf

1 of 13

sqd

1 of 13

Syp

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

bru3^d09837

model of myotonic dystrophy type 1

CEA

(Auxerre-Plantié et al., 2019)

bru3^UAS.cUa

model of dilated cardiomyopathy

CEA

(Souidi et al., 2023)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

bru3^d09837

exacerbates myotonic dystrophy type 1

modeled by mbl^GD13374

(Auxerre-Plantié et al., 2019)

model of myotonic dystrophy type 1

is ameliorated by stj^JF01825

(Auxerre-Plantié et al., 2019)

bru3^KK111663

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Al-Ramahi et al., 2018)

bru3^UAS.cUa

model of dilated cardiomyopathy

is ameliorated by Mp^HMJ21668

(Souidi et al., 2023)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CELF5; CUGBP Elav-like family member 5

13 of 14

612680

CELF3; CUGBP Elav-like family member 3

12 of 14

612678

CELF6; CUGBP Elav-like family member 6

12 of 14

612681

CELF4; CUGBP Elav-like family member 4

11 of 14

612679

CELF1; CUGBP Elav-like family member 1

6 of 14

601074

CELF2; CUGBP Elav-like family member 2

6 of 14

602538

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 97; DEE97

developmental and epileptic encephalopathy 97

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

bru3

electrophoretic mobility shift assay, autoradiography, inferred by author

(Delaunay et al., 2004)

bru3 - CG7766

experimental knowledge based

(Guruharsha et al., 2011)

bru3 - CG8475

experimental knowledge based

(Guruharsha et al., 2011)

bru3 - Faa

experimental knowledge based

(Guruharsha et al., 2011)

bru3 - PhKγ

experimental knowledge based

(Guruharsha et al., 2011)

bru3 - coro

experimental knowledge based

(Guruharsha et al., 2011)

bru3 - mRpL1

experimental knowledge based

(Guruharsha et al., 2011)

bru3 - γTub37C

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

puc|gang-of-four

suppressible

bru3

(Beam and Moberg, 2010)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264001

MIST (protein-protein) - An integrated Molecular Interaction Database

39527

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

gene_with_stop_codon_read_through

(FlyBase Genome Annotators, 2020, FlyBase Genome Annotators, 2019-)

nuclear_gene

protein_coding_gene

(FlyBase Genome Annotators, 2007)

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-41

Cytogenetic map

70B4-70C4

Sequence location

3L:13,521,907..13,803,400 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

70B4-70C4

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

70C1-70C2

(determined by in situ hybridisation)

(Spradling et al., 1999)

70C1-70C2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-41

(FlyBase, 2016)

3-34.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (66)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Korea

12145

P{VT030303-GAL4}attP2

12682

P{VT030307-GAL4}attP2

12683

P{VT030345-GAL4}attP2

12937

P{VT030306-GAL4}attP2

13477

P{VT030294-GAL4}attP2

13478

P{VT030305-GAL4}attP2

13479

P{VT030322-GAL4}attP2

14868

P{VT030297-GAL4}attP2

14933

P{VT030323-GAL4}attP2

15157

P{VT030279-GAL4}attP2

VDRC

v102442

P{KK111663}VIE-260B

v109946

P{KK114804}VIE-260B

More stocks available...

Genomic Clones (126)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (17)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD31834

Other clones

DPiM_LD31834

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0264001

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Picchio et al., 2018)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

New annotation (CG34243) in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

Relationship to Other Genes

Source for database merge of

Source for merge of: bru-3 CG10046 Annotations CG12478, CG10046 merged as CG12478 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of: bru-3 l(3)05871

(Beam and Moberg, 2010)

Source for merge of: bru-3 CG34243

(FlyBase Genome Annotators, 2012)

Additional comments

Annotations CG12478 and CG34243 merged as CG43744 in release 5.44 of the genome annotation.

(FlyBase Genome Annotators, 2012)

"gfr" mutant alleles do not behave as simple loss-of-function mutations: they are lethal in trans to each other, yet completely viable and without obvious phenotypes in trans to all available deficiencies and lethal lesions mapping to the 70A-70B chromosomal region (to which the "gfr" complementation group maps). Evidence suggests that "gfr" alleles behave as gain-of-function lesions and that overexpression of "bru-3" is central to the "gfr" mutant phenotype.

(Beam and Moberg, 2010)

Nomenclature History

Source for database identify of

Source for identity of: bru-3 CG12478

(Good et al., 2000)

Source for identity of: bru3 bru-3

(FlyBase, 2009-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

BRU-3

(Ray et al., 2013)

Bru-3

(Picchio et al., 2018, Vlasova-St Louis et al., 2013, Kandul and Noor, 2009, Delaunay et al., 2004)

CG10046

(Alias and Clark, 2007, Robertson, 2001.5.6, Good, 2000.3.24)

CG12478

(Ellis and Carney, 2011, Keleman et al., 2009.8.5, Bandyopadhyay et al., 2006, Robertson, 2001.5.6)

CG34243

(Remolina et al., 2012, Keleman et al., 2009.8.5, FlyBase Genome Annotators, 2007)

CG43744

(FlyBase Genome Annotators, 2012, Ni et al., 2010.12.1)

Dm Bru-3

(Horb and Horb, 2010, Good et al., 2000)

bru-3

(Lee et al., 2022, Huang et al., 2020, Kim et al., 2019, Al-Ramahi et al., 2018, McMahon et al., 2016, Duff et al., 2015, Gene Disruption Project members, 2015-, Kern et al., 2015, Ashwal-Fluss et al., 2014, Vernes, 2014, Smibert et al., 2012, Bellen et al., 2011, Ellis and Carney, 2011, Hartmann et al., 2011, Beam and Moberg, 2010, Stofanko et al., 2008)

bru3

(Stewart et al., 2024, Fanara et al., 2023, Li et al., 2023, Perlegos et al., 2023, Praschberger et al., 2023, Clarembaux-Badell et al., 2022, Saha et al., 2022, Michki et al., 2021, FlyBase Genome Annotators, 2020, Knudsen et al., 2020, Auxerre-Plantié et al., 2019, Fingerhut et al., 2019, FlyBase Genome Annotators, 2019-, Shih et al., 2019, Trcek and Lehmann, 2019, Lobell et al., 2017)

bruno-3

(Dalton et al., 2010)

cg10046

(Lasko, 2000)

cg12478

(Lasko, 2000)

l(3)05871

Name Synonyms

Bruno-3

(Rumpf et al., 2014)

bruno 3

bruno-3

(Beam and Moberg, 2010, Kandul and Noor, 2009, Stofanko et al., 2008, Burnette et al., 2005, Burnette et al., 2005, Good et al., 2000)

bruno3

(Vicente-Crespo et al., 2008)

lethal (3) 05871

Secondary FlyBase IDs

FBgn0262350
FBgn0036379
FBgn0036378
FBgn0010879
FBgn0085272

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 103 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39527

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A6M3Q6L8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A6M3Q6L8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0264001

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39527/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264001

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264001

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG43744

MARRVEL_MODEL - MARRVEL (model organism gene)

39527

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0264001

DroID - A comprehensive database of gene and protein interactions.

FBgn0264001

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0264001

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12478

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031941

MIST (protein-protein) - An integrated Molecular Interaction Database

39527

References (111)

The following external sites may use different assemblies or annotations than FlyBase.

-0.66

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