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Gene Dmel\tgo

General Information
Symbol	Dmel\tgo	Species	D. melanogaster
Name	tango	Annotation symbol	CG11987
Feature type	protein_coding_gene	FlyBase ID	FBgn0264075
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	dARNT, Arnt, HIF-1
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	85C2-85C2	Sequence location	3R:4,842,495..4,845,744 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene tango is referred to in FlyBase by the symbol Dmel\tgo (CG11987, FBgn0264075). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific DNA binding transcription factor activity; protein binding; sequence-specific DNA binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity; protein heterodimerization activity. There is experimental evidence for 12 unique biological process terms, many of which group under: biological regulation; single-organism developmental process; system development; regulation of RNA metabolic process; multicellular organismal development; neuron differentiation; cellular component organization or biogenesis; localization; response to hormone stimulus; cellular response to decreased oxygen levels. 31 alleles are reported. The phenotypes of these alleles are annotated with: organ system; organ system subdivision; late extended germ band embryo; non-connected developing system; peripheral nervous system; tracheal branch primordium; multicellular structure; portion of tissue; tracheal system; dorsal trunk primordium; dorsal branch primordium; eo support cell; lamina forming neuroblast; cell part; ventral nerve cord primordium; embryonic/larval neuron; adult segment. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Myc-type, basic helix-loop-helix (bHLH) domain; Nuclear translocator; PAC motif; PAS domain; PAS fold; PAS fold-3. Gene sequence location is 3R:4842495..4845744.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 85C2-85C2   Limits computationally determined from genome sequence between P{lacW}l(3)L4740L4740 and P{EP}D1EP473
Experimentally Determined Cytological Location
Cytogenetic map Notes References   Located on 3R.   (Salzberg et al., 1994) 85C-85C     (Sonnenfeld, 1997.8.21) 86D1-86D8     (Ma, 1999.5.26) 85C1-85D2   (determined by in situ hybridisation)   (Frigerio et al., 1986)
Experimentally Determined Recombination Data
Location	3- (Salzberg et al., 1994) 3-44 +/- 2 (Hummel et al., 1999)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\tgo for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) tgo-RA FBtr0082005  NM_169254   3108   642 tgo-RB FBtr0336755  NM_001275468   2634   642
Additional Transcript Data & Comments
Reported size (kB)	3.079 (longest cDNA) (Zelzer et al., 1997) 2.620 (longest cDNA) (Sonnenfeld et al., 1997) 2.355 (longest cDNA) (Ohshiro and Saigo, 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein tgo-PA   FBpp0081483   71.5   642   7.02     NP_731308   AAF54329 tgo-PB   FBpp0307731   71.5   642   7.02     NP_001262397   AGB95779
Additional Polypeptide Data & Comments
Reported size (kDa)	644 (aa) (Sonnenfeld et al., 1997) 642 (aa) (Ohshiro and Saigo, 1997) 631 (aa) (Zelzer et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • PAS domain (IPR000014) Nuclear translocator (IPR001067) Myc-type, basic helix-loop-helix (bHLH) domain (IPR011598) PAS fold-3 (IPR013655) PAS fold (IPR013767) PAC motif (IPR001610)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246656   AA246797   AA263488   AA264595   AA390402   AA391026   AA696044   AA699074   AA816633   AA951522   AA978728   AA990674   AE014297 AAF54329   AB002556 BAA22868   AF016053 AAB69695   AF020426 AAB88882   AF154417 AAD38396   AE014297 AGB95779   AI292666   AI515662   AW943114   AY069581 AAL39726   BF490246   BF504899   BI172675   BI233097   BI235852   BI366347   BI371720   BI374274   BI588255   BI605307   BQ103580   CO177507   CO192859   CO265904   CO268564   CO271777   CO274358   CO281542   CO291552   CO305286   CO307838   CO308555   CO308569   CO309597   CO313902   CO322343   CO325662   CO330923   CO331536   CO342232   EC059582   EC061968   EC065288   EC074961   EC084435   EC089041   EC199301   EC205190   EC212329   EC213747   EC214612   EC227726   EC237280   EC238218   EC239502   EC241496   EC243939   EC246478   EC247829   EC254807   EC255456   EV579736   EV584783   GH739447   GH740041   GH740625   GH742063   GH763001   GH764104   GH765562   GH765851   GH766092   GH767743   GH767777   GH768350   GH768725   M14550 AAA28838
UniProtKB/Swiss-Prot	O15945
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism | ubiquitous   (Zelzer et al., 1997) embryonic gnathal segment   (Emmons et al., 1999) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 1 -- 5   organism | ubiquitous & uniform   (Ohshiro and Saigo, 1997) embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9   organism | ubiquitous   (Ohshiro and Saigo, 1997) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11   tracheal pit   (Ohshiro and Saigo, 1997) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) tracheal primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   Bolwig organ   (Fisher et al., 2012) embryonic brain   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) embryonic stage 14   embryonic mandibular segment   (Emmons et al., 1999) embryonic maxillary segment   (Emmons et al., 1999) embryonic antennal segment   (Emmons et al., 1999) embryonic labial segment   (Emmons et al., 1999) presumptive embryonic/larval central nervous system   (Ohshiro and Saigo, 1997) embryonic stage 15   presumptive embryonic/larval peripheral nervous system   (Emmons et al., 1999) ventral thoracic disc primordium   (Emmons et al., 1999) microinjection Stage Tissue/Position (including subcellular localization) Reference oogenesis     (Frigerio et al., 1986) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 14     (Frigerio et al., 1986) Comment:reference states transcript expressed up to 8-12 hr AEL
Additional Descriptive Data
	In addition to the midline and tracheal expression, tgo is expressed in the antennal segment, the gnathal segments, the leg anlage, and the PNS in embryos. (Emmons et al., 1999) tgo is ubiquitously expressed during embryogenesis. Elevated levels were observed in tracheal placodes and tracheal pits. (Zelzer et al., 1997) tgo expression was detected in oocytes and embyros up to 8-12 hr of development. (Frigerio et al., 1986) tgo is ubiquitously distributed in early embryos. Strong signals are transiently detected in tracheal pits at stage 11. Later, strong expression is observed in the CNS. (Ohshiro and Saigo, 1997)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   embryonic/larval posterior spiracle   (Sonnenfeld et al., 1997) ventral nerve cord   (Sonnenfeld et al., 1997) supraesophageal ganglion   (Sonnenfeld et al., 1997) organism | ubiquitous   (Sonnenfeld et al., 1997) embryonic stage 11   tracheal pit | surrounding   (Sonnenfeld et al., 1997) embryonic stage 11 -- 17   embryonic/larval tracheal system   (Sonnenfeld et al., 1997) embryonic stage 14   embryonic mandibular segment   (Emmons et al., 1999) embryonic labial segment   (Emmons et al., 1999) embryonic antennal segment   (Emmons et al., 1999) embryonic maxillary segment   (Emmons et al., 1999) embryonic stage 15   embryonic/larval tracheal system   (Emmons et al., 1999) dorsal vessel primordium   (Emmons et al., 1999) presumptive embryonic/larval peripheral nervous system   (Emmons et al., 1999)
Additional Descriptive Data
	In embryos, in addition to the midline and tracheal expression, tgo is expressed in the antennal segment, the gnathal segments, the leg anlage, the PNS, and the dorsal vessel. (Emmons et al., 1999) tgo is uniformly distributed in preblastoderm embryos. During the extended germ band stage, tgo protein is found in all three germ layers. Enhanced levels of tgo protein are found around the forming tracheal pits. Enhanced levels of tgo protein continue to be observed in tracheal cells including the posterior spiracles from stage 11 to 17. Enhanced levels are also observed in the supraoesophageal ganglion and the ventral nerve cord. (Sonnenfeld et al., 1997)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Jiang and Crews, 2003, Jiang and Crews, 2007) protein complex inferred from physical interaction with dys (Ooe et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    61 central nervous system, pupae P8    42 head, virgin 1-day female    14 head, virgin 4-day female    16 head, virgin 20-day female    14 head, mated 1-day female    15 head, mated 4-day female    10 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    19 head, mated 20-day male    21 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    18 carcass, larvae L3 wandering    31 carcass, 1-day adult    11 carcass, 4-day adult    11 carcass, 20-day adult    12 ovary, virgin 4-day female    54 ovary, mated 4-day female    61 testis, mated 4-day male    9 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (48) central nervous system, larvae L3  (61) central nervous system, pupae P8  (42) head, virgin 1-day female    14 head, virgin 4-day female    16 head, virgin 20-day female    14 head, mated 1-day female    15 head, mated 4-day female    10 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    19 head, mated 20-day male    21 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    18 carcass, larvae L3 wandering  (31) carcass, 1-day adult    11 carcass, 4-day adult    11 carcass, 20-day adult    12 ovary, virgin 4-day female  (54) ovary, mated 4-day female  (61) testis, mated 4-day male    9 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    61 central nervous system, pupae P8    42 head, virgin 1-day female    14 head, virgin 4-day female    16 head, virgin 20-day female    14 head, mated 1-day female    15 head, mated 4-day female    10 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    19 head, mated 20-day male    21 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    18 carcass, larvae L3 wandering    31 carcass, 1-day adult    11 carcass, 4-day adult    11 carcass, 20-day adult    12 ovary, virgin 4-day female    54 ovary, mated 4-day female    61 testis, mated 4-day male    9 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    61 central nervous system, pupae P8    42 head, virgin 1-day female    14 head, virgin 4-day female    16 head, virgin 20-day female    14 head, mated 1-day female    15 head, mated 4-day female    10 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    19 head, mated 20-day male    21 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    18 carcass, larvae L3 wandering    31 carcass, 1-day adult    11 carcass, 4-day adult    11 carcass, 20-day adult    12 ovary, virgin 4-day female    54 ovary, mated 4-day female    61 testis, mated 4-day male    9 accessory gland, mated 4-day male    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    61 central nervous system, pupae P8    42 head, virgin 1-day female    14 head, virgin 4-day female    16 head, virgin 20-day female    14 head, mated 1-day female    15 head, mated 4-day female    10 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    19 head, mated 20-day male    21 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    18 carcass, larvae L3 wandering    31 carcass, 1-day adult    11 carcass, 4-day adult    11 carcass, 20-day adult    12 ovary, virgin 4-day female    54 ovary, mated 4-day female    61 testis, mated 4-day male    9 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (48) central nervous system, larvae L3  (61) central nervous system, pupae P8  (42) head, virgin 1-day female    14 head, virgin 4-day female    16 head, virgin 20-day female    14 head, mated 1-day female    15 head, mated 4-day female    10 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    19 head, mated 20-day male    21 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    18 carcass, larvae L3 wandering  31 carcass, 1-day adult    11 carcass, 4-day adult    11 carcass, 20-day adult    12 ovary, virgin 4-day female  (54) ovary, mated 4-day female  (61) testis, mated 4-day male    9 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    61 central nervous system, pupae P8    42 head, virgin 1-day female    14 head, virgin 4-day female    16 head, virgin 20-day female    14 head, mated 1-day female    15 head, mated 4-day female    10 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    19 head, mated 20-day male    21 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    18 carcass, larvae L3 wandering    31 carcass, 1-day adult    11 carcass, 4-day adult    11 carcass, 20-day adult    12 ovary, virgin 4-day female    54 ovary, mated 4-day female    61 testis, mated 4-day male    9 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    61 central nervous system, pupae P8    42 head, virgin 1-day female    14 head, virgin 4-day female    16 head, virgin 20-day female    14 head, mated 1-day female    15 head, mated 4-day female    10 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    19 head, mated 20-day male    21 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    7 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    5 fat body, white prepupae    14 fat body, pupae P8    18 carcass, larvae L3 wandering    31 carcass, 1-day adult    11 carcass, 4-day adult    11 carcass, 20-day adult    12 ovary, virgin 4-day female    54 ovary, mated 4-day female    61 testis, mated 4-day male    9 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    122 embryo 04-06hr    114 embryo 06-08hr    99 embryo 08-10hr    84 embryo 10-12hr    64 embryo 12-14hr    71 embryo 14-16hr    64 embryo 16-18hr    47 embryo 18-20hr    34 embryo 20-22hr    35 embryo 22-24hr    33 larva L1    14 larva L2    11 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    26 white prepupae 12hr    25 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    18 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    24 adult female 05day    34 adult female 30day    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (59) embryo 02-04hr  (122) embryo 04-06hr  (114) embryo 06-08hr  (99) embryo 08-10hr  (84) embryo 10-12hr  (64) embryo 12-14hr  (71) embryo 14-16hr  (64) embryo 16-18hr  (47) embryo 18-20hr  (34) embryo 20-22hr  (35) embryo 22-24hr  (33) larva L1    14 larva L2    11 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new  26 white prepupae 12hr    25 white prepupae 24hr  (32) pupae 2d postWPP    25 pupae 3d postWPP    18 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    24 adult female 05day  (34) adult female 30day  (35) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr  (122) embryo 04-06hr  (114) embryo 06-08hr    99 embryo 08-10hr    84 embryo 10-12hr    64 embryo 12-14hr    71 embryo 14-16hr    64 embryo 16-18hr    47 embryo 18-20hr    34 embryo 20-22hr    35 embryo 22-24hr    33 larva L1    14 larva L2    11 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    26 white prepupae 12hr    25 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    18 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    24 adult female 05day    34 adult female 30day    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    122 embryo 04-06hr    114 embryo 06-08hr    99 embryo 08-10hr    84 embryo 10-12hr    64 embryo 12-14hr    71 embryo 14-16hr    64 embryo 16-18hr    47 embryo 18-20hr    34 embryo 20-22hr    35 embryo 22-24hr    33 larva L1    14 larva L2    11 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    26 white prepupae 12hr    25 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    18 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    24 adult female 05day    34 adult female 30day    35 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    122 embryo 04-06hr    114 embryo 06-08hr    99 embryo 08-10hr    84 embryo 10-12hr    64 embryo 12-14hr    71 embryo 14-16hr    64 embryo 16-18hr    47 embryo 18-20hr    34 embryo 20-22hr    35 embryo 22-24hr    33 larva L1    14 larva L2    11 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    26 white prepupae 12hr    25 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    18 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    24 adult female 05day    34 adult female 30day    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (59) embryo 02-04hr  (122) embryo 04-06hr  (114) embryo 06-08hr  (99) embryo 08-10hr  (84) embryo 10-12hr  (64) embryo 12-14hr  (71) embryo 14-16hr  (64) embryo 16-18hr  (47) embryo 18-20hr  (34) embryo 20-22hr  (35) embryo 22-24hr  (33) larva L1    14 larva L2    11 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new  26 white prepupae 12hr    25 white prepupae 24hr  32 pupae 2d postWPP    25 pupae 3d postWPP    18 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    24 adult female 05day  (34) adult female 30day  (35) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr  122 embryo 04-06hr  114 embryo 06-08hr    99 embryo 08-10hr    84 embryo 10-12hr    64 embryo 12-14hr    71 embryo 14-16hr    64 embryo 16-18hr    47 embryo 18-20hr    34 embryo 20-22hr    35 embryo 22-24hr    33 larva L1    14 larva L2    11 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    26 white prepupae 12hr    25 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    18 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    24 adult female 05day    34 adult female 30day    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    59 embryo 02-04hr    122 embryo 04-06hr    114 embryo 06-08hr    99 embryo 08-10hr    84 embryo 10-12hr    64 embryo 12-14hr    71 embryo 14-16hr    64 embryo 16-18hr    47 embryo 18-20hr    34 embryo 20-22hr    35 embryo 22-24hr    33 larva L1    14 larva L2    11 larva L3 12hr old    7 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    20 white prepupae new    26 white prepupae 12hr    25 white prepupae 24hr    32 pupae 2d postWPP    25 pupae 3d postWPP    18 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    12 adult female 01day    24 adult female 05day    34 adult female 30day    35 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    14 embryonic 1182-4H    30 embryonic GM2    30 embryonic Kc167    29 embryonic S1    38 embryonic S3    25 leg disc CME L1    23 wing disc CME-W2    20 wing disc ML-DmD8    25 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    14 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    25 ovary fGS/OSS    43 ovary OSC    33 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (30) Schneider line 2 Sg4    14 embryonic 1182-4H  (30) embryonic GM2  (30) embryonic Kc167  (29) embryonic S1  (38) embryonic S3    25 leg disc CME L1    23 wing disc CME-W2    20 wing disc ML-DmD8    25 wing disc ML-DmD9  (37) wing disc ML-DmD16-c3  (31) wing disc ML-DmD21    14 wing disc ML-DmD32  (36) haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11  (36) antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1  (49) CNS ML-DmBG2-c2  (32) tumorous blood cells mbn2    25 ovary fGS/OSS  (43) ovary OSC  (33) ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    14 embryonic 1182-4H    30 embryonic GM2    30 embryonic Kc167    29 embryonic S1    38 embryonic S3    25 leg disc CME L1    23 wing disc CME-W2    20 wing disc ML-DmD8    25 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    14 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    25 ovary fGS/OSS    43 ovary OSC    33 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    14 embryonic 1182-4H    30 embryonic GM2    30 embryonic Kc167    29 embryonic S1    38 embryonic S3    25 leg disc CME L1    23 wing disc CME-W2    20 wing disc ML-DmD8    25 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    14 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    25 ovary fGS/OSS    43 ovary OSC    33 ovary OSS    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    14 embryonic 1182-4H    30 embryonic GM2    30 embryonic Kc167    29 embryonic S1    38 embryonic S3    25 leg disc CME L1    23 wing disc CME-W2    20 wing disc ML-DmD8    25 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    14 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    25 ovary fGS/OSS    43 ovary OSC    33 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  30 Schneider line 2 Sg4    14 embryonic 1182-4H  30 embryonic GM2  30 embryonic Kc167  29 embryonic S1  (38) embryonic S3    25 leg disc CME L1    23 wing disc CME-W2    20 wing disc ML-DmD8    25 wing disc ML-DmD9  (37) wing disc ML-DmD16-c3  31 wing disc ML-DmD21    14 wing disc ML-DmD32  (36) haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11  (36) antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1  (49) CNS ML-DmBG2-c2  32 tumorous blood cells mbn2    25 ovary fGS/OSS  (43) ovary OSC  (33) ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    14 embryonic 1182-4H    30 embryonic GM2    30 embryonic Kc167    29 embryonic S1    38 embryonic S3    25 leg disc CME L1    23 wing disc CME-W2    20 wing disc ML-DmD8    25 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    14 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    25 ovary fGS/OSS    43 ovary OSC    33 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    14 embryonic 1182-4H    30 embryonic GM2    30 embryonic Kc167    29 embryonic S1    38 embryonic S3    25 leg disc CME L1    23 wing disc CME-W2    20 wing disc ML-DmD8    25 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    14 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    24 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    21 CNS ML-DmBG1-c1    49 CNS ML-DmBG2-c2    32 tumorous blood cells mbn2    25 ovary fGS/OSS    43 ovary OSC    33 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    36 cold shock, 4-day adult    23 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    45 Caffeine 25 mg/ml 48 hrs, 4-day adult    28 Paraquat 5 mM 48 hrs, 4-day adult    37 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (36) cold shock, 4-day adult    23 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult  (42) Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult  (27) Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3  (46) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (45) Caffeine 25 mg/ml 48 hrs, 4-day adult  (28) Paraquat 5 mM 48 hrs, 4-day adult  (37) Paraquat 10 mM 48 hrs, 4-day adult  (35) Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    36 cold shock, 4-day adult    23 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    45 Caffeine 25 mg/ml 48 hrs, 4-day adult    28 Paraquat 5 mM 48 hrs, 4-day adult    37 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    36 cold shock, 4-day adult    23 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    45 Caffeine 25 mg/ml 48 hrs, 4-day adult    28 Paraquat 5 mM 48 hrs, 4-day adult    37 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    36 cold shock, 4-day adult    23 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    45 Caffeine 25 mg/ml 48 hrs, 4-day adult    28 Paraquat 5 mM 48 hrs, 4-day adult    37 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (36) cold shock, 4-day adult    23 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult  (42) Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult  27 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3  (46) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (45) Caffeine 25 mg/ml 48 hrs, 4-day adult  28 Paraquat 5 mM 48 hrs, 4-day adult  (37) Paraquat 10 mM 48 hrs, 4-day adult  (35) Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    36 cold shock, 4-day adult    23 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    45 Caffeine 25 mg/ml 48 hrs, 4-day adult    28 Paraquat 5 mM 48 hrs, 4-day adult    37 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    36 cold shock, 4-day adult    23 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    10 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    12 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    19 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    45 Caffeine 25 mg/ml 48 hrs, 4-day adult    28 Paraquat 5 mM 48 hrs, 4-day adult    37 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD32037
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive tgo1 tgo2 tgo3 tgoF261 tgoJ68 viable, with Scer\GAL4pnr-MD237 tgoGD4497 Other Phenotypes Allele cell migration defective tgoA1-42 tgoC8 cell migration defective, with Scer\GAL4loco.1.3 tgoDN.Scer\UAS neuroanatomy defective tgo3 tgoA1-42 tgoC8 neuroanatomy defective, with Scer\GAL4btl.PS tgoΔc.Scer\UAS neuroanatomy defective, with Scer\GAL4loco.1.3 tgoDN.Scer\UAS neuroanatomy defective, with Scer\GAL4NP6099 tgoDN.Scer\UAS neuroanatomy defective, with Scer\GAL4sim.P3.7 tgoΔc.Scer\UAS visible, with Scer\GAL4pnr-MD237 tgoGD4497 visible | recessive | somatic clone tgo1 tgo5 tgo6 Phenotype manifest in Allele antenna, with Scer\GAL4Dll-em212 tgodsRNA.Scer\UAS antenna | somatic clone tgo6 tgo5 tgo1 arista | somatic clone tgo5 tgo1 axon tgo1 basal cylinder | somatic clone tgo5 tgo1 commissure tgounspecified commissure, with Scer\GAL4sim.P3.7 tgoΔc.Scer\UAS dorsal branch primordium tgo3 dorsal trunk primordium tgo3 embryonic/larval brain, with Scer\GAL4NP6099 tgoDN.Scer\UAS embryonic/larval tracheal system tgo1 embryonic/larval tracheal system, with Scer\GAL4btl.PS tgoDN.Scer\UAS exit glial cell tgoA1-42 tgoC8 exit glial cell, with Scer\GAL4loco.1.3 tgoDN.Scer\UAS ganglionic branch primordium tgo3 glial cell, with Scer\GAL4loco.1.3 tgoDN.Scer\UAS intersegmental nerve tgoA1-42 tgoC8 lamina forming neuroblast, with Scer\GAL4NP6099 tgoDN.Scer\UAS leg | distal | somatic clone tgo5 tgo1 leg | ectopic, with Scer\GAL4Dll-em212 tgodsRNA.Scer\UAS longitudinal connective tgounspecified tgoA1-42 tgoC8 longitudinal connective, with Scer\GAL4sim.P3.7 tgoΔc.Scer\UAS macrochaeta | somatic clone tgo6 tgo5 tgo1 maxillary palpus | somatic clone tgo5 tgo1 midline glial cell tgo1 tgounspecified midline glial cell, with Scer\GAL4btl.PS tgoΔc.Scer\UAS neuroblast MNB tgo1 peripheral nervous system tgoF261 tgoJ68 presumptive embryonic/larval central nervous system tgounspecified presumptive embryonic/larval tracheal system tgo3 tgo5 pretarsus | somatic clone tgo1 segmental nerve tgoA1-42 tgoC8 subperineurial glial cell tgoA1-42 tgoC8 tarsal segment 2 | somatic clone tgo5 tarsal segment 3 | somatic clone tgo5 tarsal segment 4 | somatic clone tgo5 tgo1 tarsal segment | somatic clone tgo1 third segment of antenna | distal | somatic clone tgo5 tgo1 tracheal system, with Scer\GAL4btl.PS tgoΔc.Scer\UAS transverse connective primordium tgo3 trichogen cell, with Scer\GAL4pnr-MD237 tgoGD4497 unguis | ectopic | somatic clone tgo5 tgo1 VUM neuron tgo1 wing | somatic clone tgo6 tgo5 tgo1
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of tgo Class Mutagen Stocks Known lesion tgo1 hypomorphic allele - genetic evidence ethyl methanesulfonate 1 Yes tgo5 1 -- tgoEY03802 P-element activity 1 -- tgoF261 ethyl methanesulfonate 1 -- tgo2 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes tgo3 ethyl methanesulfonate 0 Yes tgo6 0 -- tgo7 0 -- tgoA1-42 ethyl methanesulfonate 0 -- tgoC8 ethyl methanesulfonate 0 -- tgoDG08708 0 -- tgoJ68 ethyl methanesulfonate 0 -- tgoR65 Delta2-3 0 Yes tgoR91 Delta2-3 0 Yes tgoR94 Delta2-3 0 Yes tgounspecified 0 --
Alleles Carried on Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Allele of tgo Class Mutagen Stocks Known lesion tgoGD4497 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tgoGL01042 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tgoJF02302 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes tgoScer\UAS.cWa in vitro construct - regulatory fusion 1 Yes tgoAct5C.PS in vitro construct - regulatory fusion 0 Yes tgoDN.Scer\UAS in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis in vitro construct - amino acid replacement 0 Yes tgodsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes tgoNIG.11987R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes tgoScer\UAS.cSa in vitro construct - regulatory fusion 0 Yes tgoΔc.Scer\UAS in vitro construct - deletion in vitro construct - regulatory fusion 0 Yes tgoΔPRD+.Act5C in vitro construct - deletion in vitro construct - regulatory fusion 0 Yes tgoΔPRD.Act5C in vitro construct - deletion in vitro construct - regulatory fusion 0 Yes tgoΔQ123.Act5C in vitro construct - deletion in vitro construct - regulatory fusion 0 Yes tgoΔQ23.Act5C in vitro construct - deletion in vitro construct - regulatory fusion 0 Yes tgoΔQ3.Act5C in vitro construct - deletion in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6258 (Ryder, 2004.4.26) Df(3R)p-XT9 (Sonnenfeld et al., 1997)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4497} NA P{NIG.11987R} NA P{TRiP.GL01042} NA P{TRiP.JF02302} NA P{UAS-tgo.DN} NA P{UAS-tgo.IR} NA P{UAS-tgo.S} NA P{UAS-tgo.W} NA P{UAS-tgo.Δc} NA characterization construct P{HIF1A/ARNT-lacZ} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}tgoEY03802 NA insertion of enhancer trap P{lacW}tgoR65 No P{lacW}tgoR91 No P{lacW}tgoR94 No miscellaneous insertions P{wHy}tgoDG08708 NA
Gene Ontology: Function, Process & Cellular Component ( 28 unique terms )
Terms Based on Experimental Evidence ( 20 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Myo10A (Liu et al., 2008) protein heterodimerization activity inferred from physical interaction with dys (Ooe et al., 2007, Jiang and Crews, 2007) inferred from physical interaction with sim (Sonnenfeld et al., 1997) inferred from physical interaction with sima (Sonnenfeld et al., 1997) inferred from physical interaction with ss (Kozu et al., 2006) contributes_to RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription inferred from physical interaction with sim (Sonnenfeld et al., 1997) inferred from physical interaction with trh (Sonnenfeld et al., 1997) contributes_to RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity inferred from direct assay (Kozu et al., 2006) sequence-specific DNA binding inferred from direct assay (Kozu et al., 2006, Jiang and Crews, 2007) contributes_to sequence-specific DNA binding inferred from direct assay (Ooe et al., 2007) contributes_to sequence-specific DNA binding transcription factor activity inferred from direct assay (Ooe et al., 2007) inferred from physical interaction with dys (Jiang and Crews, 2007) inferred from physical interaction with sim (Jiang and Crews, 2007) inferred from physical interaction with trh (Jiang and Crews, 2007)
Biological Process
CV term Evidence References
brain development inferred from mutant phenotype (Umetsu et al., 2006) cellular response to hypoxia inferred from direct assay (Dekanty et al., 2005) cellular response to insulin stimulus inferred from direct assay (Dekanty et al., 2005) central nervous system development inferred from mutant phenotype (Hummel et al., 1999) dendrite morphogenesis inferred from mutant phenotype (Parrish et al., 2006) glial cell migration inferred from mutant phenotype (Schmidt et al., 2011) muscle organ development inferred from mutant phenotype (Parrish et al., 2006) negative regulation of transcription from RNA polymerase II promoter inferred from mutant phenotype (Kozu et al., 2006) neuron development inferred from mutant phenotype (Parrish et al., 2006) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Ooe et al., 2007) regulation of transcription, DNA-dependent inferred from direct assay (Sonnenfeld et al., 1997) regulation of transcription from RNA polymerase II promoter inferred from direct assay (Jiang and Crews, 2007)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Jiang and Crews, 2003, Jiang and Crews, 2007) protein complex inferred from physical interaction with dys (Ooe et al., 2007)
Terms Based on Predictions or Assertions ( 11 terms )
Molecular Function
CV term Evidence References
protein heterodimerization activity traceable author statement (Jiang and Crews, 2003, Ghabrial et al., 2003) sequence-specific DNA binding RNA polymerase II transcription factor activity inferred from sequence or structural similarity with human ARNT (Sonnenfeld et al., 1997) sequence-specific DNA binding transcription factor activity traceable author statement (Ghabrial et al., 2003) signal transducer activity inferred from electronic annotation with InterPro:IPR000014 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
epithelial cell fate determination, open tracheal system non-traceable author statement (Affolter and Shilo, 2000) traceable author statement (Ghabrial et al., 2003) open tracheal system development traceable author statement (Zelzer and Shilo, 2000) peripheral nervous system development traceable author statement (Salzberg et al., 1994) signal transduction inferred from electronic annotation with InterPro:IPR000014, InterPro:IPR001610 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
cytoplasm inferred from electronic annotation with InterPro:IPR001067 (FlyBase Curators et al., 2004-) nucleus inferred by curator from GO:0043565|GO:0003700 (Ooe et al., 2007) inferred from sequence or structural similarity with human ARNT (Sonnenfeld et al., 1997) transcription factor complex inferred from electronic annotation with InterPro:IPR001067 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
tgo - sim experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions tgo allele Gene References tgo1 jing (Sedaghat et al., 2002) trh (Sonnenfeld et al., 1997, Sedaghat et al., 2002) tgo5 piwi (Smulders-Srinivasan and Lin, 2003) tgoScer\UAS.cWa trh (Sonnenfeld et al., 2005) unspecified btl (Sedaghat et al., 2002) sim (Umetsu et al., 2006, Sedaghat et al., 2002) ss (Emmons et al., 1999)
External Data
Linkouts	SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • O15945
Orthologs
OrthoDB Orthologs (60) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6QRHPN Dipteran inclusive ortholog search EOG6CRM7D Insect inclusive ortholog search EOG6V6XC3 Arthropod inclusive ortholog search EOG66DJJ5 Metazoa inclusive ortholog search EOG6VB6HJ
Orthologs in Drosophila Species (EOG6QRHPN)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\tgo     Drosophila simulans   Dsim\GD20825 Y   Drosophila sechellia   Dsec\GM26289 Y   Drosophila erecta   Dere\GG17399 Y   Drosophila yakuba   Dyak\GE24803 Y   Drosophila ananassae   Dana\GF18433 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA11313 Y   Drosophila persimilis   Dper\GL12493 Y   Drosophila willistoni   Dwil\GK11420 Y   Drosophila virilis   Dvir\GJ10876 Y   Drosophila mojavensis   Dmoj\GI10175 Y   Drosophila grimshawi   Dgri\GH18221 Y
Orthologs in non-Drosophila Dipterans (EOG6CRM7D)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010343   Anopheles gambiae Malaria mosquito  Agam\AGAP009748   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ014773
Orthologs in non-Dipteran Insects (EOG6V6XC3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB43953 Y Apis mellifera Western honey bee  Amel\GB44259 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007066 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004242 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH27111 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11686 Y Atta cephalotes Leafcutter ant  Acep\ACEP27210 Y Atta cephalotes Leafcutter ant  Acep\ACEP18972 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO22839 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO23030 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14283 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL22140 Y Linepithema humile Argentine ant  Lhum\LH15139 Y Linepithema humile Argentine ant  Lhum\LH16078 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB23402 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB17601 Y Solenopsis invicta Red fire ant  Sinv\SINV21271 Y Solenopsis invicta Red fire ant  Sinv\SINV17782 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008301 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI004686 Y Bombyx mori Silkmoth  Bmor\BGIBMGA003472 Y Bombyx mori Silkmoth  Bmor\BGIBMGA003870 Y Pediculus humanus Human body louse  Phum\PHUM505970 Y Pediculus humanus Human body louse  Phum\PHUM580780 Y Tribolium castaneum Red flour beetle  Tcas\cycle Y Tribolium castaneum Red flour beetle  Tcas\TC004710 Y
Orthologs in non-Insect Arthropods (EOG66DJJ5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\tub2 Y Daphnia pulex Water flea  Dpux\matK Y Ixodes scapularis Deer tick  Isca\ISCW023999 Y
Orthologs in non-Arthropod Metazoa (EOG6VB6HJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\aha-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000129gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_022971gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019518gn   Danio rerio Zebrafish  Drer\arnt2   Danio rerio Zebrafish  Drer\arnt   Xenopus tropicalis Western clawed frog  Xtro\arnt   Xenopus tropicalis Western clawed frog  Xtro\arnt2   Gallus gallus Domestic chicken  Ggal\ARNT2   Gallus gallus Domestic chicken  Ggal\ENSGALG00000000751   Mus musculus House mouse  Mmus\Arnt2   Mus musculus House mouse  Mmus\Arnt   Rattus norvegicus Norway rat  Rnor\Arnt2   Rattus norvegicus Norway rat  Rnor\Arnt   Homo sapiens Human  Hsap\ARNT2   Homo sapiens Human  Hsap\ARNT
Human Orthologs (2)
Gene OMIM HGNC Hsap\ARNT2     Hsap\ARNT
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20825 Drosophila sechellia Dsec\GM26289 Drosophila erecta Dere\GG17399 Drosophila yakuba Dyak\GE24803 Drosophila ananassae Dana\GF18433 Drosophila pseudoobscura pseudoobscura Dpse\GA11313 Drosophila persimilis Dper\GL12493 Drosophila willistoni Dwil\GK11420 Drosophila virilis Dvir\GJ10876 Drosophila mojavensis Dmoj\GI10175 Drosophila grimshawi Dgri\GH18221
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	9583 w*; P{UAS-tgo.W}2 9589 P{neoFRT}82B tgo5/TM1 26740 y1 v1; P{TRiP.JF02302}attP2 38928 y1 sc* v1; P{TRiP.GL01042}attP2 9588 w*; ru1 P{neoFRT}82B tgo1 e1/TM3, Sb1 15914 y1 w67c23; P{EPgy2}tgoEY03802/TM3, Sb1 Ser1 4388 y1 w1; tgoF261, P{lacW}64A th1 st1 cu1 sr1 es ca1/TM6B, P{iab-2(1.7)lacZ}6B, Tb+
VDRC	v10735 w1118; P{GD4497}v10735
Genomic Clones ( 2 )
	BACR02G22 BACR09F04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 59 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD32037
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT07020 AT26564 GH15619 GM04955 HL07418 LD02932 LD05646 LD05787 LD06769 LD08274 LD09315 LD10023 LD32749 LD34487 LD47567 RE15319 RE29376 RE31796 RE51863 RE58667 RE61826 RH29404 RH70888
Other clones	Dmel_dig_008467_0583_2172 Dmel_dig_013199_0719_0840 EC34653 EK053712 EK091941 EK104603 EK113861 EK144415 EK157414 EK187929 EK197043 EK197625 EK218518 EK234254 EK247961 EK255823 EK258709 EK258737 EK296439 EK300404 EP14224 RP001006475 RP001006641 RP001019244 RP001020915 RP001025081 RP001029989 RP001030093 RP001037572 RP001046671 RP001048711 RP001053091 RP001054515 RP001056033 UUGC0988
RNAi & Array Information
Linkouts
Antibody Information
	monoclonal (Sonnenfeld et al., 1997)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: tgo jay (Schmidt et al., 2011)
Additional comments
Other Comments
	tgo has a cell-autonomous function in the peripheral glia. (Schmidt et al., 2011) dsRNA has been made from templates generated with primers directed against this gene. RNAi of tgo results in reduced arborization of ddaD and ddaE neurons, defects in muscle, alterations in the number of MD neurons, defects in dendrite morphogenesis. However, RNAi causes no obvious defects in da dendrite development. (Parrish et al., 2006) ss and tgo proteins interact to control antennal and tarsal development. (Emmons et al., 1999) Two EMS induced alleles were identified in a screen for mutations affecting commissure formation in the CNS of the embryo. (Hummel et al., 1999) Nuclear localisation of the tgo protein is dependent on the presence of either sim or trh protein, and nuclear entry of sim or trh protein requires interaction with tgo protein. (Ward et al., 1998) tgo has been cloned and sequenced. Isolated form an embryonic cDNA library using a human hARNT cDNA as a probe. (Ohshiro and Saigo, 1997) btl expression in the developing trachea is regulated by direct interactions between trh/tgo heterodimers and three central midline elements located in the btl minimum enhancer. (Ohshiro and Saigo, 1997) sim and trh activate transcription by forming dimers with the tgo protein. Gene dosage studies reveal in vivo interactions between sim and tgo, and trh and tgo. The interacting proteins in vivo control CNS midline and tracheal transcription and development. tgo protein is found broadly distributed throughout embryogenesis, although certain cell types, including trachea and the CNS have enhanced levels. (Sonnenfeld et al., 1997) Mutants cause CNS and tracheal defects. (Sonnenfeld et al., 1997) jay is required for PNS development in the embryo. (Salzberg et al., 1994) Mutation in jay affects neuronal connections, affecting pathway finding. (Salzberg et al., 1994) Identified on the basis of similarity to a 600bp prd cDNA fragment which codes for the last 205 amino acids of prd. (Frigerio et al., 1986)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_169254 NM_001275468 RefSeq (Proteins) • NP_731308 NP_001262397 DDBJ / EMBL / GenBank • AA951522 AAA28838 AAB69695 AAB88882 AAD38396 AAF54329 AAL39726 AB002556 AF016053 AF020426 AF154417 AGB95779 AW943114 AY069581 BAA22868 M14550 Entrez Gene - A searchable database of RefSeq genes. • 41084 UniProtKB/Swiss-Prot • O15945
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • PAS domain (IPR000014) Nuclear translocator (IPR001067) Myc-type, basic helix-loop-helix (bHLH) domain (IPR011598) PAS fold-3 (IPR013655) PAS fold (IPR013767) PAC motif (IPR001610) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD32037
Linkouts
	SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • O15945
Synonyms & Secondary IDs ( 24 )
Reported As
Symbol Synonym	Arnt (Hirayama and Sassone-Corsi, 2005, Feyereisen, 1998, Ashok et al., 1998, ) ARNT (Hendricks, 2003, Driscoll and Gerstbrein, 2003, Ma, 1999.5.26, van Gelder and Krasnow, 1996, Crews et al., 1995) arnt (Zelzer, 1997.7.25) bHLHe4 (Stevens et al., 2008) CG11987 (Liu et al., 2008, Stevens et al., 2008, Stevens et al., 2008, Perkins et al., 2009.3.31, Ni et al., 2010.12.1) dARNT (Weiss et al., 2010, Wolf et al., 2001, Ohshiro, 1997.4.1, Hahn, 1998, Darlington et al., 1998, Ohshiro and Saigo, 1997, Umetsu et al., 2006) DARNT (Zelzer and Shilo, 2000, Bacon et al., 1998, Shilo et al., 1997, Zelzer et al., 1997) darnt (Sonnenfeld et al., 1997) dHIF-1β (van Uden et al., 2011) dTgo (Ooe et al., 2007) Hif1 (Shaw et al., 2002) HIF-1 (Baird et al., 2006, Lee et al., 2008) HIF1b   HIF-1β (Driscoll and Gerstbrein, 2003) jay   prd7   Tango (Crews and Fan, 1999) tgo (Schmidt et al., 2011, Doronkin et al., 2010, Mukherjee et al., 2011, Weake et al., 2011, Crews and Brenman, 2006, Kim et al., 2006, Jiang and Crews, 2007, Weiler, 2007, Harvey et al., 2008, Tajiri et al., 2007, Dekanty et al., 2005, Céspedes et al., 2010, Choi et al., 2007, Japanese National Institute of Genetics, 2012.5.21, Gurudatta et al., 2012) Tgo (Morozova et al., 2010, Sonnenfeld et al., 2010, Dekanty et al., 2007, Umetsu et al., 2006, Jiang and Crews, 2006, Parrish et al., 2006, Reig et al., 2007, Ooe et al., 2007, Romero et al., 2008, Jiang and Crews, 2003, Chung et al., 2009, Michaud and Tanguay, 2003, Mortimer and Moberg, 2007) TGO (Haberman et al., 2001)
Name Synonym	jaywalker (Hummel et al., 1999, Salzberg et al., 1994) paired-like 7   PRD gene 7 (Frigerio et al., 1986) Tango (Dekanty et al., 2010, Morozova et al., 2010, Sonnenfeld et al., 2010, Kudo et al., 2009, Dekanty et al., 2007, Reig et al., 2007, Romero et al., 2008, Michaud and Tanguay, 2003, Choi et al., 2007, Fulkerson and Estes, 2011, Mortimer and Moberg, 2007) tango (van Uden et al., 2011, Liu et al., 2008, Kozu et al., 2006, Weiler, 2007, Harvey et al., 2008, Tajiri et al., 2007, Dekanty et al., 2005, Schmidt et al., 2011)
Secondary FlyBase IDs
	FBgn0003147 FBgn0011605 FBgn0015014
References ( 129 )
Generate a list of	All references relating to this gene ( 129 )
List References by type	Research paper  ( 65 ) Supplementary material  ( 6 ) Review  ( 20 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 17 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 4 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 )
Recent research papers ( 6 )
	Gurudatta et al., 2012, Dev. Biol. 369(1): 124--132 The BEAF insulator regulates genes involved in cell polarity and neoplastic growth. [FBrf0219796] Fulkerson and Estes, 2011, J. Exp. Zool. B Mol. Dev. Evol. 316B(1): 61--75 Common motifs shared by conserved enhancers of Drosophila midline glial genes. [FBrf0212527] Mukherjee et al., 2011, Science 332(6034): 1210--1213 Interaction between Notch and Hif-alpha in development and survival of Drosophila blood cells. [FBrf0214249] Schmidt et al., 2011, Glia 59(9): 1264--1272 Transcriptional regulation of peripheral glial cell differentiation in the embryonic nervous system of drosophila. [FBrf0214270] van Uden et al., 2011, PLoS Genet. 7(1): e1001285 Evolutionary Conserved Regulation of HIF-1β by NF-κB. [FBrf0212948] Weake et al., 2011, Genes Dev. 25(14): 1499--1509 Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation. [FBrf0214372] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...