SLO-binding protein
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Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Gene model reviewed during 5.44
Interacts specifically with Slo; which activates Slo activity. Interacts with 14-3-3-zeta when phosphorylated. Forms a heterotetrameric complex containing phosphorylated Slob, Slo and 14-3-3-zeta, which represses Slo activity due to the indirect interaction between Slo and 14-3-3-zeta.
Phosphorylated. Phosphorylation of Ser-54 and Ser-79 is required for the interaction with 14-3-3-zeta but not with that of Slo.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Slob using the Feature Mapper tool.
Slob is detected in at last two of the three subsets of PI neurons, PI-1 and PI-3 by RT-PCR.
A probe common to all Slob transcripts detects expression in the adult photoreceptors, optic lobe, and brain cortex. One isoform is found preferentially in the optic lobes, particularly in the medulla and in the brian cortex but not in photoreceptors. Another isoform is seen in the eye and to a lesser extent in the optic lobe and brian cortex.
Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.
Comment: peak at ZT 10-14
Slob staining is seen in nerve and nerve terminals and to a more limited extent in muscle at neuromuscular junctions.
Staining with an antibody to a particular Slob isoform (Slob71/65) is observed in the optic lobe and large ventral lateral neurons (I-LNvs) and less intense staining in the surrounding brain cortex. Punctate staining is seen in the medulla.
Slob protein expression exhibtions a circadian pattern, with an expression peak at ZT 10-14, and a trough at ZT 2. Slob immunoreactivty is intense in the nuclei of photoreceptor cells and basal cells of the eye. In the adult brain, bright and discrete cytoplasmic immunoreactivity is observed in 6-8 neurons of the pars intercerebralis, while more diffuse staining is observed in optic lobes and brain cortex. Slob protein exhibits circadian oscillation; in the nuclei of photoreceptor cells, protein levels peak at ZT 14-21, and trough at ZT 2, while in the pars intercerbralis neurons, the peak is at ZT 3 and trough at ZT 15-21.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Slob in GBrowse 22-27
2-24.7
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: Slob CG13792
Annotations CG6772 and CG13792 merged as CG43756 in release 5.44 of the genome annotation. Merge supported by RNA-Seq junction and coverage data.
Slob protein shows weak and barely detectable protein kinase activity in vitro. Slob protein kinase activity is markedly enhanced when Slob protein is pretreated with the catalytic subunit of cyclic AMP-dependent protein kinase (PKAc). The major site of phosphorylation by PKAc within Slob is serine 54.
Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per01, tim01 and ClkJrk background.
Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.
Identification: Protein that binds to the carboxy-terminal domain of slo in a yeast two-hybrid screen. Slob and slo co-immunoprecipitate from heads and heterologous host cells, suggesting they interact in vivo. Slob also co-immunoprecipitates with eag but not with Sh. Slob is a potassium channel-binding protein that influences multiple channel properties.
