Immuno-EM staining of stage 16 embryos identified sli
protein on the lumenal surface of the hindgut boundary cells, which correlated in size and shape to microvilli structures.
is detected in class 1 and class IV dendritic arborizing neurons in larvae. It is also detected in muscles and in some other dendritic arborizing neurons.
protein is observed in a sheet of cells in the midline of the developing subesophageal ganglion.
protein is enriched in the extracellular matrix of S2 cells.
protein was detected in the body wall epidermis in a broad pattern in the dorsal and lateral aspect in embryonic stages 13-15. sli
protein could no longer be detected at stage 16 in the epidermis. Expression of sli
in the central nervous system midline was detectable from stages 13-16.
transcript and protein expression patterns coincide. sli
protein is first detected during gastrulation. At germ band extension, midline mesectodermal cells express sli
protein. During germ band retraction, sli
protein is detected in the 6 midline glial cells derived from the midline neurepithelium. During dorsal closure, sli
protein expression is detected in cardioblasts, walls of the gut, and near the muscle attachment sites in the ectoderm. Using immunoelectron microscopy, sli
protein is detected along the axons of commissural and longitudinal axon tracts, but not in the cell bodies supplying these axons, suggesting that sli
protein is secreted from the midline glial cells and becomes associated with the axons which traverse the midline.
protein is first detected in the midline neurepithelium after germ band extension. During germ band shortening, expression becomes restricted to median ectodermal cells (MECs). After germ band contraction is complete, sli
protein is detected in MECs, the axon commissures they surround, and longitudinal connectives. In supraesophageal segments, strong axon labelling is detected, and this labelling seems to be on the cell surface.