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General Information
Symbol
Dmel\PolE1
Species
D. melanogaster
Name
DNA polymerase epsilon subunit 1
Annotation Symbol
CG6768
Feature Type
FlyBase ID
FBgn0264326
Gene Model Status
Stock Availability
Enzyme Name (EC)
DNA-directed DNA polymerase (2.7.7.7)
Gene Summary
DNA polymerase epsilon subunit 1 (PolE1) encodes the large subunit of DNA pol epsilon, an essential DNA polymerase participating with DNA pol alpha and DNA pol delta in cellular DNA replication. The C-terminal and N-terminal regions of the products of PolE1 have differential requirements in mitotic and endo-replicating cells. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)
Also Known As

DNApol-epsilon, DNApol-ε, DNA polymerase ε, DNApol-ε255, l(3)pl10

Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:23,348,128..23,355,163 [+]
Recombination map
3-78
RefSeq locus
NT_033777 REGION:23348128..23355163
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (18 terms)
Molecular Function (7 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from physical interaction with UniProtKB:Q9VIT0
(assigned by UniProt )
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
enables DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR006134, InterPro:IPR006172
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR013697
(assigned by InterPro )
Biological Process (9 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
involved_in DNA repair
inferred from mutant phenotype
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from mutant phenotype
involved_in mitotic cell cycle
inferred from mutant phenotype
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(assigned by GO_Central )
involved_in mitotic cell cycle
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(assigned by GO_Central )
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:PolE2; FB:FBgn0035644
located_in nucleus
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the DNA polymerase type-B family. (Q9VCN1)
Catalytic Activity (EC)
Experimental Evidence
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (2.7.7.7)
Predictions / Assertions
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (2.7.7.7)
Summaries
Gene Snapshot
DNA polymerase epsilon subunit 1 (PolE1) encodes the large subunit of DNA pol epsilon, an essential DNA polymerase participating with DNA pol alpha and DNA pol delta in cellular DNA replication. The C-terminal and N-terminal regions of the products of PolE1 have differential requirements in mitotic and endo-replicating cells. [Date last reviewed: 2019-09-26]
Gene Group (FlyBase)
DNA POLYMERASE EPSILON -
DNA polymerase epsilon functions as a leading strand polymerase during nuclear DNA synthesis. It is a heterotetramer in which DNApol-ε255 encodes the catalytic subunit with polymerase and 3'-5' exonuclease proofreading activities. (Adapted from PMID:30005324.)
DNA POLYMERASE CATALYTIC SUBUNITS -
Multiple DNA polymerases are required for DNA replication and/or repair in eukaryotic cells. The alpha-primase, delta, epsilon, zeta and gamma polymerases exist as multi-subunit complexes in which one of the subunits has catalytic activity, whereas other DNA polymerases act as single molecules. (Adapted from PMID:30005324.)
3'-5'-EXODEOXYRIBONUCLEASES -
3'-5'-exodeoxyribonucleases catalyze the sequential degradation of double-stranded DNA acting progressively in a 3'-to-5' direction, releasing nucleoside 5'-phosphates.
Protein Function (UniProtKB)
Catalytic component of the DNA polymerase epsilon complex (PubMed:15135399). Participates in chromosomal DNA replication (PubMed:15135399). Required during synthesis of the leading DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork (By similarity). Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication (PubMed:15135399). Has a role in the G1-S transition and/or S-phase progression of the mitotic cycle and endocycle progression (PubMed:11054539, PubMed:15135399, PubMed:21898761). Involved in DNA synthesis during DNA repair (By similarity). Plays roles in larval tissue development (PubMed:22245183, PubMed:21898761).
(UniProt, Q9VCN1)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\PolE1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Differences between protein sequence and SWP:Q9GV40 are in the center of a long ORF and may correspond to strain differences.

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0084408
6849
2236
Additional Transcript Data and Comments
Reported size (kB)

8.0 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0083800
256.7
2236
6.42
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

2220 (aa); 255 (kD predicted)

250 (kD observed)

Comments

DNApol-ε protein was purified and characterized.

External Data
Subunit Structure (UniProtKB)

Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit DNApol-epsilon255/DNApolE1 and the accessory subunits DNApol-epsilon58/DNApolE2, Chrac-14/DNApolE3 and Mes4/DNApolE4.

(UniProt, Q9VCN1)
Domain

The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for maintenance of the complex.

The C-terminal domain (1001-2236 aa) is required for mitotic DNA replication in the eye but not for DNA endoreplication in salivary glands.

The CysA-type zinc finger is required for PCNA-binding.

The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.

(UniProt, Q9VCN1)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\PolE1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:PolE2; FB:FBgn0035644
located_in nucleus
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\PolE1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
EMBL-EBI Single Cell Expression Atlas
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of PolE1
Transgenic constructs containing regulatory region of PolE1
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (4)
10 of 13
Yes
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (5)
9 of 12
Yes
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (4)
14 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
15 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (2)
8 of 9
Yes
Yes
8 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (4)
14 of 15
Yes
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
11 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190077 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915003K )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W006M )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X002Z )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Stegodyphus mimosarum
African social velvet spider
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G006J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (3)
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit DNApol-epsilon255/DNApolE1 and the accessory subunits DNApol-epsilon58/DNApolE2, Chrac-14/DNApolE3 and Mes4/DNApolE4.
    (UniProt, Q9VCN1 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-78
    Cytogenetic map
    Sequence location
    3R:23,348,128..23,355,163 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    94E13-94E13
    Limits computationally determined from genome sequence between P{PZ}cnc03921 and P{PZ}l(3)0690606906
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    94E13-94F1-94E13-94F1
    94F6-95A1
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (6)
    Genomic Clones (15)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (4)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: DNApol-ε255 DNApol-ε

      Source for identity of: PolE1 DNApol-ε255

      Source for database merge of

      Source for merge of: DNApol-ε CG6768

      Additional comments

      Renamed from 'DNApol-ε' to 'DNApol-ε255' to better distinguish from the other DNApol-ε subunit (DNApol-ε58) and to be consistent with the nomenclature of other DNA polymerase genes.

      Source for merge of DNApol-ε CG6768 was sequence comparison ( date:001216 ).

      Other Comments

      Expression pattern and subcellular distribution of the protein during development is studied using antibodies.

      Origin and Etymology
      Discoverer
      Etymology

      Source for merge of: DNApol-ε l(3)pl10

      Identification
      External Crossreferences and Linkouts ( 47 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      EMBL-EBI Single Cell Expression Atlas
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL
      Synonyms and Secondary IDs (26)
      Reported As
      Name Synonyms
      DNA polymerase epsilon
      DNA polymerase epsilon 255kD subunit
      DNA polymerase epsilon subunit 1
      DNA polymerase ε 255kD subunit
      DNApol-epsilon
      DNApolymarase E
      lethal (3) pl10
      pol.ε catalytic subunit
      Secondary FlyBase IDs
      • FBgn0086326
      • FBgn0020756
      • FBgn0039072
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (61)