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Gene Dmel\Eip93F

General Information
Symbol	Dmel\Eip93F	Species	D. melanogaster
Name	Ecdysone-induced protein 93F	Annotation symbol	CG18389
Feature type	protein_coding_gene	FlyBase ID	FBgn0264490
Gene Model Status	Current	Stock availability	16 publicly available
Also Known As	E93
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	93F5-93F14	Sequence location	3R:17,774,836..17,840,261 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ecdysone-induced protein 93F is referred to in FlyBase by the symbol Dmel\Eip93F (CG18389, FBgn0264490). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: DNA binding; sequence-specific DNA binding transcription factor activity. There is experimental evidence that it is involved in the biological process: activation of cysteine-type endopeptidase activity involved in apoptotic process; hormone-mediated apoptotic signaling pathway; positive regulation of transcription from RNA polymerase II promoter; cellular response to hypoxia; cellular response to ecdysone; autophagic cell death; autophagy; salivary gland cell autophagic cell death. 28 alleles are reported. The phenotypes of these alleles are annotated with: organ system; adult segment; organ system subdivision; multicellular structure; thoracic segment; sense organ; adult; eo support cell; external compound sense organ; imaginal precursor. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: DNA binding HTH domain, Psq-type; Homeodomain-like. Gene sequence location is 3R:17774836..17840261.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 93F5-93F14   Left limit from in situ hybridisation (FBrf0054167) Right limit from in situ hybridisation (FBrf0054167); Limits computationally determined from genome sequence between P{PZ}tsl00617 and P{lacW}howj5B5&P{EP}EP738
Experimentally Determined Cytological Location
Cytogenetic map Notes References 93F7-93F14   Located by the range spanned by the 135kb chromosome walk known to include the Eip93F transcription unit.   (Baehrecke and Thummel, 1995) 93F-93F   (determined by in situ hybridisation)   (Woodard et al., 1994) 93F5-93F6   (determined by in situ hybridisation)   (Berg and Spradling, 1991)
Experimentally Determined Recombination Data
Location	3-
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Eip93F for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Stop-codon suppression (UGA) postulated; FBrf0216884. Gene model reviewed during 5.44 gene_with_stop_codon_read_through ; SO:0000697 Gene model reviewed during 5.47 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Eip93F-RA FBtr0084169  NM_143745   12020   1165 Eip93F-RB FBtr0112874  NM_001104395   4438   1188 Eip93F-RC FBtr0330384  NM_001275888   12020   1188
Additional Transcript Data & Comments
Reported size (kB)	9.5 (northern blot) (Baehrecke and Thummel, 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Eip93F-PA   FBpp0083567   124.0   1165   7.15     NP_652002   AAF55940 Eip93F-PB   FBpp0111787   126.8   1188   7.41     NP_001097865   ABW08726 Eip93F-PC   FBpp0303410   126.5   1188   7.12     NP_001262817   AGB96197
Additional Polypeptide Data & Comments
Reported size (kDa)	1221 (aa); 146 (kD) (Baehrecke and Thummel, 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • DNA binding HTH domain, Psq-type (IPR007889) Homeodomain-like (IPR009057)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA698275   AA698277   AE014297 AAF55940   AE014297 ABW08726   AE014297 AGB96197   AI133835   AI295148   AX664484   BT010034 AAQ22503   BT031093   CA804514   CA804723   CA805939   CO178703   CO265707   CO271673   CO281390   CO284202   CO284344   CO284645   CO285738   CO290674   CO292995   CO293299   CO296517   CO300322   CO309122   CO310004   CO310295   CO318890   CO323615   CO327310   CO327742   CO331304   CO331868   CO332578   CO333212   CO333981   CO345417   CZ471293   CZ484070   CZ484244   CZ484245   CZ490809   CZ490810   EC052727   EC053700   EC053705   EC056235   EC061391   EC061958   EC064642   EC066232   EC066345   EC066953   EC067748   EC067848   EC069936   EC070392   EC195854   EC201233   EC205451   EC210063   EC212168   EC213353   EC216696   EC220467   EC223030   EC236221   EC240712   EC246060   EC247092   EC253701   EV579010   EV584861   EV595707   U25686 AAA83228
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7YU18 Q9VD60 Q24079
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   amnioserosa   (Fisher et al., 2012) embryonic stage 13 -- 16   amnioserosa   (Fisher et al., 2012) prepupal stage P4(ii)   embryonic/larval salivary gland   (Baehrecke and Thummel, 1995) Comment:reference states 12 hr APF adult stage   antennal sense organ | subset   (Jafari et al., 2012) adult olfactory receptor neuron Or92a | subset   (Jafari et al., 2012) adult olfactory receptor neuron Or47b | subset   (Jafari et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference prepupal stage | early   pupal/adult digestive system   (Baehrecke and Thummel, 1995) embryonic/larval fat body   (Baehrecke and Thummel, 1995) prepupal stage | late   imaginal disc   (Baehrecke and Thummel, 1995) embryonic/larval central nervous system   (Baehrecke and Thummel, 1995) embryonic/larval salivary gland   (Baehrecke and Thummel, 1995) pupal/adult digestive system   (Baehrecke and Thummel, 1995) embryonic/larval fat body   (Baehrecke and Thummel, 1995) prepupal stage P1 -- pupal stage P7   organism   (Baehrecke and Thummel, 1995) Comment:0-40 hr APF pharate adult stage P15(i)     (Baehrecke and Thummel, 1995) Comment:reference states 96 hr APF
Additional Descriptive Data
	Eip93F transcripts are first detected during metamorphosis on northern blots. Transcript levels increase following the late larval and prepupal ecdysteroid pulses, as well as immediately before adult eclosion. In dissected salivary glands, they are expressed only in prepupal glands and were shown to be induced by 20E. In situ hybridization and northern blot analysis of RNA isolated from dissected tissues shows that Eip93F transcripts are present in gut and at a lower level in fat body of early prepupae and in the CNS, gut, fat body, salivary glands and imaginal discs in late prepupae. (Baehrecke and Thummel, 1995) Eip93F transcript is expressed in a subset of olfactory sense organs in the antenna. These include some sensilla labelled by Mmus\Cd8a[Or47b.330.T:Avic\GFP] or Mmus\Cd8a[Or92a.Avic\GFP.T:Hsap\MYC]. (Jafari et al., 2012) RNA blots were carried out on RNA extracted from staged larval and prepupal salivary glands. Eip93F transcripts are induced in apparent response to the prepupal ecdysone pulse paralleling the puffing response at 93F. Ectopic ftz-f1 expression leads to Eip93F induction in late-larval salivary glands. (Woodard et al., 1994)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Lee et al., 2000) colocalizes_with transcriptionally active chromatin inferred from direct assay (Lee et al., 2000)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    29 central nervous system, pupae P8    25 head, virgin 1-day female    26 head, virgin 4-day female    23 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    20 head, mated 20-day female    13 head, mated 1-day male    31 head, mated 4-day male    33 head, mated 20-day male    24 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    5 digestive system, 4-day adult    8 digestive system, 20-day adult    6 fat body, larvae L3 wandering    0 fat body, white prepupae    14 fat body, pupae P8    14 carcass, larvae L3 wandering    3 carcass, 1-day adult    17 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3  (29) central nervous system, pupae P8    25 head, virgin 1-day female  26 head, virgin 4-day female    23 head, virgin 20-day female    10 head, mated 1-day female  26 head, mated 4-day female    20 head, mated 20-day female    13 head, mated 1-day male  (31) head, mated 4-day male  (33) head, mated 20-day male    24 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    5 digestive system, 4-day adult    8 digestive system, 20-day adult    6 fat body, larvae L3 wandering    0 fat body, white prepupae    14 fat body, pupae P8    14 carcass, larvae L3 wandering    3 carcass, 1-day adult    17 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    29 central nervous system, pupae P8    25 head, virgin 1-day female    26 head, virgin 4-day female    23 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    20 head, mated 20-day female    13 head, mated 1-day male    31 head, mated 4-day male    33 head, mated 20-day male    24 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    5 digestive system, 4-day adult    8 digestive system, 20-day adult    6 fat body, larvae L3 wandering    0 fat body, white prepupae    14 fat body, pupae P8    14 carcass, larvae L3 wandering    3 carcass, 1-day adult    17 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    29 central nervous system, pupae P8    25 head, virgin 1-day female    26 head, virgin 4-day female    23 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    20 head, mated 20-day female    13 head, mated 1-day male    31 head, mated 4-day male    33 head, mated 20-day male    24 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    5 digestive system, 4-day adult    8 digestive system, 20-day adult    6 fat body, larvae L3 wandering    0 fat body, white prepupae    14 fat body, pupae P8    14 carcass, larvae L3 wandering    3 carcass, 1-day adult    17 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    29 central nervous system, pupae P8    25 head, virgin 1-day female    26 head, virgin 4-day female    23 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    20 head, mated 20-day female    13 head, mated 1-day male    31 head, mated 4-day male    33 head, mated 20-day male    24 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    5 digestive system, 4-day adult    8 digestive system, 20-day adult    6 fat body, larvae L3 wandering    0 fat body, white prepupae    14 fat body, pupae P8    14 carcass, larvae L3 wandering    3 carcass, 1-day adult    17 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3  29 central nervous system, pupae P8    25 head, virgin 1-day female  26 head, virgin 4-day female    23 head, virgin 20-day female    10 head, mated 1-day female  26 head, mated 4-day female    20 head, mated 20-day female    13 head, mated 1-day male  31 head, mated 4-day male  (33) head, mated 20-day male    24 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    5 digestive system, 4-day adult    8 digestive system, 20-day adult    6 fat body, larvae L3 wandering    0 fat body, white prepupae    14 fat body, pupae P8    14 carcass, larvae L3 wandering    3 carcass, 1-day adult    17 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    29 central nervous system, pupae P8    25 head, virgin 1-day female    26 head, virgin 4-day female    23 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    20 head, mated 20-day female    13 head, mated 1-day male    31 head, mated 4-day male    33 head, mated 20-day male    24 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    5 digestive system, 4-day adult    8 digestive system, 20-day adult    6 fat body, larvae L3 wandering    0 fat body, white prepupae    14 fat body, pupae P8    14 carcass, larvae L3 wandering    3 carcass, 1-day adult    17 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    29 central nervous system, pupae P8    25 head, virgin 1-day female    26 head, virgin 4-day female    23 head, virgin 20-day female    10 head, mated 1-day female    26 head, mated 4-day female    20 head, mated 20-day female    13 head, mated 1-day male    31 head, mated 4-day male    33 head, mated 20-day male    24 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    8 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    5 digestive system, 4-day adult    8 digestive system, 20-day adult    6 fat body, larvae L3 wandering    0 fat body, white prepupae    14 fat body, pupae P8    14 carcass, larvae L3 wandering    3 carcass, 1-day adult    17 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    1 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    4 white prepupae new    20 white prepupae 12hr    44 white prepupae 24hr    26 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    6 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    4 white prepupae new    20 white prepupae 12hr  (44) white prepupae 24hr  26 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    6 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    4 white prepupae new    20 white prepupae 12hr    44 white prepupae 24hr    26 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    6 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    4 white prepupae new    20 white prepupae 12hr    44 white prepupae 24hr    26 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    6 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    4 white prepupae new    20 white prepupae 12hr    44 white prepupae 24hr    26 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    6 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    4 white prepupae new    20 white prepupae 12hr  (44) white prepupae 24hr  26 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    6 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    4 white prepupae new    20 white prepupae 12hr    44 white prepupae 24hr    26 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    6 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    0 embryo 16-18hr    0 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    4 white prepupae new    20 white prepupae 12hr    44 white prepupae 24hr    26 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    6 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    1 embryonic 1182-4H    1 embryonic GM2    13 embryonic Kc167    7 embryonic S1    1 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    13 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    1 ovary fGS/OSS    27 ovary OSC    9 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    1 embryonic 1182-4H    1 embryonic GM2    13 embryonic Kc167    7 embryonic S1    1 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    13 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2  (39) tumorous blood cells mbn2    1 ovary fGS/OSS  (27) ovary OSC    9 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    1 embryonic 1182-4H    1 embryonic GM2    13 embryonic Kc167    7 embryonic S1    1 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    13 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    1 ovary fGS/OSS    27 ovary OSC    9 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    1 embryonic 1182-4H    1 embryonic GM2    13 embryonic Kc167    7 embryonic S1    1 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    13 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    1 ovary fGS/OSS    27 ovary OSC    9 ovary OSS    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    1 embryonic 1182-4H    1 embryonic GM2    13 embryonic Kc167    7 embryonic S1    1 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    13 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    1 ovary fGS/OSS    27 ovary OSC    9 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    1 embryonic 1182-4H    1 embryonic GM2    13 embryonic Kc167    7 embryonic S1    1 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    13 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2  (39) tumorous blood cells mbn2    1 ovary fGS/OSS  27 ovary OSC    9 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    1 embryonic 1182-4H    1 embryonic GM2    13 embryonic Kc167    7 embryonic S1    1 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    13 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    1 ovary fGS/OSS    27 ovary OSC    9 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    1 embryonic 1182-4H    1 embryonic GM2    13 embryonic Kc167    7 embryonic S1    1 embryonic S3    0 leg disc CME L1    1 wing disc CME-W2    0 wing disc ML-DmD8    13 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    1 ovary fGS/OSS    27 ovary OSC    9 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    21 Ethanol 2.5% 3 hrs, larvae L3    5 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_04 mE1_20_mRNA_expression_cluster_19
External Data & Images
Linkouts	BDGP expression data • GH10557
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4hs.PB Eip93FScer\UAS.cLa lethal | pair rule expression pattern, with Scer\GAL4h-1J3 Eip93FScer\UAS.cLa lethal | P-stage | recessive Eip93F4 Eip93F5 Eip93F6 lethal | pupal stage, with Scer\GAL4pnr-MD237 Eip93FGD4449 lethal | pupal stage, with Scer\GAL4vg.PM Eip93FScer\UAS.cLa lethal | recessive Eip93Fry93 partially lethal - majority die | pupal stage Eip93F1 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Eip93FGD4449 partially lethal - majority die | pupal stage (with Df(3R)93Fx2) Eip93F1 partially lethal - majority die | pupal stage (with Eip93F1) Eip93F2 Eip93F3 partially lethal - majority die | pupal stage (with Eip93F2) Eip93F1 partially lethal - majority die | pupal stage (with Eip93F3) Eip93F1 viable Eip93Ff08111 Sterility Allele fertile Eip93Ff08111 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 Eip93FGD4449 decreased cell death Eip93F1 Eip93F2 Eip93FdsRNA.cKa increased cell death, with Scer\GAL4hs.PB Eip93FScer\UAS.cLa increased cell death, with Scer\GAL4vg.PM Eip93FScer\UAS.cLa increased cell death | pair rule expression pattern, with Scer\GAL4h-1J3 Eip93FScer\UAS.cLa visible, with Scer\GAL4pnr-MD237 Eip93FGD4449 visible | somatic clone Eip93F4 Phenotype manifest in Allele abdomen Eip93F5 Eip93F6 antenna Eip93F5 Eip93F6 antennal sense organ Eip93F4 bract Eip93F4 bract | somatic clone Eip93F4 embryo, with Scer\GAL4h-1J3 Eip93FScer\UAS.cLa embryo, with Scer\GAL4hs.PB Eip93FScer\UAS.cLa embryonic/larval midgut | prepupal stage (with Df(3R)93Fx2) Eip93F1 embryonic/larval salivary gland Eip93F2 Eip93F3 embryonic/larval salivary gland (with Df(3R)93Fx2) Eip93F1 eye Eip93F4 Eip93F5 Eip93F6 leg Eip93F5 Eip93F6 mesothoracic tergum, with Scer\GAL4pnr-MD237 Eip93FGD4449 pupa Eip93F1 pupa (with Df(3R)93Fx2) Eip93F1 pupa (with Eip93F1) Eip93F2 Eip93F3 pupa (with Eip93F2) Eip93F1 pupa (with Eip93F3) Eip93F1 salivary gland Eip93F1 Eip93F2 tergite Eip93F4 trichogen cell, with Scer\GAL4pnr-MD237 Eip93FGD4449 wing Eip93F5 Eip93F6 wing disc, with Scer\GAL4vg.PM Eip93FScer\UAS.cLa wing vein Eip93F4
Classical Alleles ( 20 )
For All Classical Alleles Show
Allele of Eip93F Class Mutagen Stocks Known lesion Eip93Ff08111 piggyBac transposase 2 -- Eip93Fc02710a 1 -- Eip93Fc03082a 1 -- Eip93Fc07152 piggyBac transposase 1 -- Eip93Fd07598 1 -- Eip93Ff01667 1 -- Eip93Ff01771 piggyBac transposase 1 -- Eip93FG7133 P-element activity 1 -- Eip93FMI05200 1 -- Eip93Fry93 P-element activity 1 Yes Eip93F1 loss of function allele ethyl methanesulfonate 0 Yes Eip93F2 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- Eip93F3 hypomorphic allele - genetic evidence 0 -- Eip93F4 0 Yes Eip93F5 0 Yes Eip93F6 0 Yes Eip93Ff01603 piggyBac transposase 0 -- Eip93Ff08110 piggyBac transposase 0 -- Eip93Funspecified 0 -- Eip93FVno 0 Yes
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Eip93F Class Mutagen Stocks Known lesion Eip93FGD4449 in vitro construct - RNAi in vitro construct - regulatory fusion 4 Yes Eip93FKK108140 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Eip93FdsRNA.cKa in vitro construct - RNAi 0 Yes Eip93FNIG.18389R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Eip93FScer\UAS.A in vitro construct - regulatory fusion 0 Yes Eip93FScer\UAS.B in vitro construct - regulatory fusion 0 Yes Eip93FScer\UAS.cLa in vitro construct - regulatory fusion 0 Yes Eip93FVDRC.cUa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)93Fx2 (Lee and Baehrecke, 2001) Df(3R)e-BS2 (Chen et al., 1996) Df(3R)Exel6188 (Ryder, 2004.4.26) Tp(3;3)Vno (Mou et al., 2012)
Not disrupted in	Df(3R)e-F1 (Chen et al., 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4449} NA P{KK108140} NA P{NIG.18389R} NA P{UAS-Eip93F.A} NA P{UAS-Eip93F.B} NA P{UAS-Eip93F.L} NA characterization construct P{VDRC.Eip93F.U} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}Eip93FMI05200 NA P{ry11}Eip93Fry93 NA PBac{PB}Eip93Fc02710a NA PBac{PB}Eip93Fc03082a NA PBac{PB}Eip93Fc07152 NA PBac{WH}Eip93Ff01603 NA PBac{WH}Eip93Ff01667 NA PBac{WH}Eip93Ff01771 NA PBac{WH}Eip93Ff08110 NA PBac{WH}Eip93Ff08111 NA insertion of mobile activating element P{EP}Eip93FG7133 NA P{XP}Eip93Fd07598 NA
Gene Ontology: Function, Process & Cellular Component ( 18 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
activation of cysteine-type endopeptidase activity involved in apoptotic process inferred from mutant phenotype (Takemoto et al., 2007) autophagic cell death inferred from expression pattern (Gorski et al., 2003) autophagy inferred from mutant phenotype (Lee and Baehrecke, 2001) cellular response to ecdysone inferred from mutant phenotype (Daish et al., 2003) cellular response to hypoxia inferred from mutant phenotype (Lee et al., 2008) hormone-mediated apoptotic signaling pathway inferred from mutant phenotype (Lee et al., 2000) positive regulation of transcription from RNA polymerase II promoter inferred from mutant phenotype (Daish et al., 2003) salivary gland cell autophagic cell death inferred from expression pattern (Gorski et al., 2003)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Lee et al., 2000) colocalizes_with transcriptionally active chromatin inferred from direct assay (Lee et al., 2000)
Terms Based on Predictions or Assertions ( 11 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR007889, InterPro:IPR011526 (FlyBase Curators et al., 2004-) non-traceable author statement (Lehmann, 2002.7.1) sequence-specific DNA binding transcription factor activity non-traceable author statement (Lafont, 2000)
Biological Process
CV term Evidence References
autophagic cell death traceable author statement (Thummel, 2001) ecdysone-mediated induction of salivary gland cell autophagic cell death traceable author statement (Buszczak and Segraves, 2000) larval midgut histolysis traceable author statement (Buszczak and Segraves, 2000) phagocytosis, engulfment traceable author statement (Thummel, 2001) positive regulation of transcription, DNA-dependent traceable author statement (Thummel, 2001) regulation of transcription, DNA-dependent non-traceable author statement (Lafont, 2000) salivary gland cell autophagic cell death non-traceable author statement (Lee and Baehrecke, 2000)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Thummel, 2001) traceable author statement (Buszczak and Segraves, 2000) polytene chromosome non-traceable author statement (Thummel, 2001) traceable author statement (Buszczak and Segraves, 2000)
Sequence Ontology: Class of Gene
	gene   gene_with_edited_transcript (Graveley et al., 2011) gene_with_stop_codon_read_through (Jungreis et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Eip93F allele Gene References Eip93F1 ftz-f1 (Lee et al., 2002)
External Data
Linkouts
Orthologs
OrthoDB Orthologs (7) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6Z3717 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6Z3717)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Eip93F     Drosophila simulans   Dsim\GD20912 Y   Drosophila sechellia   Dsec\GM26391 Y   Drosophila erecta   Dere\GG11097 Y   Drosophila yakuba   Dyak\GE10258 Y   Drosophila ananassae   Dana\GF18059 Y   Drosophila mojavensis   Dmoj\GI10724 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20912 Drosophila sechellia Dsec\GM26391 Drosophila erecta Dere\GG11097 Drosophila yakuba Dyak\GE10258 Drosophila ananassae Dana\GF18059 Drosophila pseudoobscura pseudoobscura Dpse\GA27060 Drosophila persimilis Dper\GL23807 Drosophila willistoni Dwil\GK14236 Drosophila virilis Dvir\GJ23138 Drosophila mojavensis Dmoj\GI10724 Drosophila grimshawi Dgri\GH20931
Stocks & Reagents
Stocks Listed in FlyBase ( 16 )
Bloomington	30179 w1118; P{EP}Eip93FG7133 19132 w1118/FM7c; PBac{WH}Eip93Ff08111
Harvard	- P{XP}Eip93Fd07598 - PBac{PB}Eip93Fc07152 - PBac{WH}Eip93Ff01667 - PBac{WH}Eip93Ff01771 - PBac{WH}Eip93Ff08111
VDRC	v104390 P{KK108140}VIE-260B v45857 w1118; P{GD4449}v45857 v45858 w1118; P{GD4449}v45858 v45855 w1118; P{GD4449}v45855/CyO v45856 w1118; P{GD4449}v45856/TM3
	More stocks available...
Genomic Clones ( 2 )
	BACR04F21 BACR08K01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 64 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH10557 LP08695
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	HL04092 HL04094
Other clones	18304 33048 42390 55944 57475 66323 72826 73306 80734 97549 98322 153809 157361 174352 274399 277284 281120 283252 CK01.22A.C5 CK01.72B.G8 CK01.77C.F2 CK01.96A.H9 CK01.170A.G5 Dmel_dig_003262_0588_1980 Dmel_dig_009071_0620_3523 Dmel_neb_001684_0605_0350 EC052727 EC053700 EC053705 EC056235 EC253701 EK057360 EK060553 EK074729 EK104227 EK143434 EK157032 EK165502 EK165836 EK166457 EK177937 EK190947 EK201364 EK202514 EK213853 EK220232 EK221033 EK260350 EK274915 EK299036 EK301860 EK303940 EK306214 EK308527 EP06747 EP07947 EP22016 ESG0114a.B21_A08 ESG0115a.B21_A01 ESG0120b.B21_F07
RNAi & Array Information
Linkouts
Antibody Information
	polyclonal (Mou et al., 2012)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Eip93F anon-WO02059370.58 (FlyBase, 1996-) Source for merge of: Eip93F CG18389 (Misra, 2000.4.6) Source for merge of: Eip93F l(3)ry93 (Mou et al., 2012)
Additional comments	Source for merge of Eip93F anon-WO02059370.58 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	Eip93F is required for salivary gland, autophagic, cell death. Eip93F is sufficient to trigger cellular changes that result in engulfment by phagocytes, and that coordination of the complete apoptotic response requires the rpr/grim/W cluster. (Lee and Baehrecke, 2001) Eip93F may be a critical regulator of spatial and temporal patterns of steroid-triggered programmed cell death during metamorphosis. (Lee et al., 2000) The Eip93F gene displays stage- and tissue-specific regulation by 20-hydroxyecdysone. (Baehrecke and Thummel, 1995) Ecdysone regulated puff gene. (Woodard et al., 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143745 NM_001104395 NM_001275888 RefSeq (Proteins) • NP_652002 NP_001097865 NP_001262817 DDBJ / EMBL / GenBank • AAA83228 AAF55940 AAQ22503 ABW08726 AGB96197 AI133835 AI295148 AX664484 BT010034 CZ471293 CZ484070 CZ484244 CZ484245 CZ490809 CZ490810 U25686 Entrez Gene - A searchable database of RefSeq genes. • 44936 UniProtKB/TrEMBL • Q7YU18 Q9VD60 Q24079
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • DNA binding HTH domain, Psq-type (IPR007889) Homeodomain-like (IPR009057) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH10557
Linkouts
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	anon-WO02059370.58   CG18389 (Kanao and Miyachi, 2006, Lee et al., 2003, Baehrecke, 2000.4.17, Junion et al., 2007, Lee et al., 2008, Keleman et al., 2009.8.5) E93 (Jafari et al., 2012, Mou et al., 2012, Kanao and Miyachi, 2006, Dialynas et al., 2010, Kilpatrick et al., 2005, Li and White, 2003, Kumar and Cakouros, 2004, Kumar et al., 2003, Thummel, 2001, Lafont, 2000, Thummel and Chory, 2002, Lee et al., 2002, Mathur et al., 2003, Lee et al., 2002, Siegmund and Lehmann, 2002, Lee et al., 2002, Simon et al., 2001, Lee et al., 2001, Lee et al., 2001, Thummel, 2001, Lee and Baehrecke, 2001, Buszczak and Segraves, 2000, Lee et al., 2000, Jiang et al., 2000, Pecasse et al., 2000, Lee and Baehrecke, 2000, Baehrecke, 1995.4.24, Lam et al., 1999, Richards et al., 1999, Broadus et al., 1999, Woodard and Gniadek, 1999, Baehrecke and Lee, 1999, D'Avino and Thummel, 1998, Baehrecke and Lee, 1998, Thummel, 1997, Baehrecke, 1996, Thummel, 1995, Henrich and Brown, 1995, Woodard et al., 1994, Gorski et al., 2003, Ruaud et al., 2010, Daish et al., 2003, Kpebe and Rabinow, 2008, Thummel, 2007, Varghese and Cohen, 2007, Wang et al., 2010, Bashirullah et al., 2007, Daish et al., 2004, Cao et al., 2007, Takemoto et al., 2007, Chittaranjan et al., 2009, Martin et al., 2007, Fan et al., 2010, Ihry et al., 2012) Eip93f (Jafari et al., 2012) Eip93F (Burgler and Macdonald, 2005, Jungreis et al., 2011, Wasbrough et al., 2010, Blanco et al., 2010, Stark et al., 2005, Ruaud et al., 2010, Zeitouni et al., 2007, Lee et al., 2008, Martin et al., 2007, Liu and Lehmann, 2008, Abruzzi et al., 2011, Japanese National Institute of Genetics, 2012.5.21) eip93f (Truscott et al., 2011) l(3)93 (Bloomington Drosophila Stock Center, 2000.1.31, ) l(3)ry93 (Mou et al., 2012, ) unnamed (Chen et al., 1996)
Name Synonym	Ecdysone-induced protein 93F   Eip93F (Zeitouni et al., 2007) lethal (3) ry93
Secondary FlyBase IDs
	FBgn0013585 FBgn0013948 FBgn0038920 FBgn0066001
References ( 104 )
Generate a list of	All references relating to this gene ( 104 )
List References by type	Research paper  ( 53 ) Supplementary material  ( 7 ) Review  ( 12 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 10 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Patent  ( 1 ) Website  ( 1 )
Recent research papers ( 6 )
	Fabian et al., 2012, Mol. Ecol. 21(19): 4748--4769 Genome-wide patterns of latitudinal differentiation among populations of Drosophila melanogaster from North America. [FBrf0219505] Ihry et al., 2012, PLoS Genet. 8(11): e1003085 Translational Control by the DEAD Box RNA Helicase belle Regulates Ecdysone-Triggered Transcriptional Cascades. [FBrf0220149] Jafari et al., 2012, PLoS Biol. 10(3): e1001280 Combinatorial activation and repression by seven transcription factors specify Drosophila odorant receptor expression. [FBrf0217822] Mou et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(8): 2949--2954 Control of target gene specificity during metamorphosis by the steroid response gene E93. [FBrf0217473] Abruzzi et al., 2011, Genes Dev. 25(22): 2374--2386 Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. [FBrf0216798] Truscott et al., 2011, Genes Dev. 25(17): 1820--1834 mir-11 limits the proapoptotic function of its host gene, dE2f1. [FBrf0215211] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...