E93, l(3)93, l(3)ry93, lincRNA.833, psg11
Stop-codon suppression (UGA) postulated; FBrf0216884.
Gene model reviewed during 5.44
gene_with_stop_codon_read_through ; SO:0000697
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
9.5 (northern blot)
None of the polypeptides share 100% sequence identity.
1221 (aa); 146 (kD)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Eip93F using the Feature Mapper tool.
Expression increases after the prepupal (+10 hr APF) ecdysone pulse.
Eip93F transcripts are first detected during metamorphosis on northern blots. Transcript levels increase following the late larval and prepupal ecdysteroid pulses, as well as immediately before adult eclosion. In dissected salivary glands, they are expressed only in prepupal glands and were shown to be induced by 20E. In situ hybridization and northern blot analysis of RNA isolated from dissected tissues shows that Eip93F transcripts are present in gut and at a lower level in fat body of early prepupae and in the CNS, gut, fat body, salivary glands and imaginal discs in late prepupae.
RNA blots were carried out on RNA extracted from staged larval and prepupal salivary glands. Eip93F transcripts are induced in apparent response to the prepupal ecdysone pulse paralleling the puffing response at 93F. Ectopic ftz-f1 expression leads to Eip93F induction in late-larval salivary glands.
Eip93F is expressed broadly in developing adult tissues during pupation, but is not expressed in larvae. In pupal wings, Eip93F expression is reduced in the wing veins in comparision to the rest of the developing wing blade. At 39h APF, Eip93F is expressed in stripes on the labellum that likely correspond to developing pseudotracheae.
On the pupal leg, Eip93F is present at 27h APF in bract, bristle precursor cells (external sensory organ precursor cells), and tormogen (socket) cells. By 30h APF, expression is limited to only bract and tormogen cells, and is further limited to only bract cells by 42h APF. In adults, bracts are found on the legs and the wings. However, in the abdomen at 42h APF, Eip93F is highly expressed in tormogen cells and "bract-equivalent cells" associated with bristle precursor (external sensory organ precursor) cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Eip93F in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Three mutant alleles ('E93', 'E93' and 'E93') that form a single complementation group and that were previously assigned to the Eip93F locus have been shown to instead be allelic to the neighbouring Idh3b gene. The alleles have been renamed Idh3b1, Idh3b2 and Idh3b3 respectively.
Source for merge of Eip93F anon-WO02059370.58 was sequence comparison ( date:051113 ).