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General Information
Symbol
Dmel\CkIIα
Species
D. melanogaster
Name
casein kinase IIα
Annotation Symbol
CG17520
Feature Type
FlyBase ID
FBgn0264492
Gene Model Status
Stock Availability
Gene Summary
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. May participate in Wnt signaling. (UniProt, P08181)
Contribute a Gene Snapshot for this gene.
Also Known As

CK2, CKII, CK2α, Tik, Timekeeper

Key Links
Genomic Location
Cytogenetic map
Sequence location
3L:23,097,072..23,103,460 [+]
Recombination map
3-47
RefSeq locus
NT_037436 REGION:23097072..23103460
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (23 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN001171790
(assigned by GO_Central )
Biological Process (16 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
inferred from mutant phenotype
involved_in chaeta development
inferred from mutant phenotype
involved_in circadian rhythm
inferred from mutant phenotype
inferred from mutant phenotype
involved_in lateral inhibition
inferred from mutant phenotype
involved_in locomotor rhythm
inferred from mutant phenotype
involved_in mitotic cell cycle
inferred from high throughput mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:orb; FB:FBgn0004882
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001171790
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001171790
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001171790
(assigned by GO_Central )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
located_in cytosol
inferred from direct assay
located_in nucleus
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in cytosol
inferred from biological aspect of ancestor with PANTHER:PTN000621946
(assigned by GO_Central )
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000621946
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000621946
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. (P08181)
Summaries
Gene Group (FlyBase)
CMGC KINASES (UNCLASSIFIED) -
CMGC family members are primarily proline-directed serine/threonine kinases. (Adapted from FBrf0132098).
Pathway (FlyBase)
Positive Regulators of Hedgehog Signaling Pathway -
Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
Protein Function (UniProtKB)
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. May participate in Wnt signaling.
(UniProt, P08181)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
CkII α: Casein kinase II, α subunit
The structural gene for the catalytic subunit of casein kinase II (CKII). CKII is a cyclic-nucleotide-independent, Ca2+ and calmodulin-insensitive protein kinase. It phosphorylates serine and threonine residues of a broad range of nuclear and non-nuclear proteins with functions in development, cell division, and oncogenesis. It exists as a 130,000 molecular weight α2β2 tetramer. Purification of the Drosophila enzyme described by Glover, Shelton, and Brutlag (1983, J. Biol. Chem. 258: 3258-65).
Gene Model and Products
Number of Transcripts
7
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\CkIIα for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0070042
1536
336
FBtr0070043
1561
336
FBtr0070044
1423
336
FBtr0111141
1581
336
FBtr0344974
2053
336
FBtr0345138
1481
336
FBtr0345139
1429
336
Additional Transcript Data and Comments
Reported size (kB)

1.467 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0070041
40.0
336
7.26
FBpp0070042
40.0
336
7.26
FBpp0070043
40.0
336
7.26
FBpp0110433
40.0
336
7.26
FBpp0311229
40.0
336
7.26
FBpp0311359
40.0
336
7.26
FBpp0311360
40.0
336
7.26
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)

336 (aa); 39.833 (kD predicted)

36.7 (kD observed)

Comments

Purified CkII protein kinases were able to phosphorylate acid-soluble proteins from Drosophila Kc cells.

External Data
Subunit Structure (UniProtKB)

Tetramer of two alpha and two beta chains.

(UniProt, P08181)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CkIIα using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
s-LNv neuron

Comment: 3 hours after ZT

mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

CkIIα is expressed in Pdf- and Clk-positive s-LNv neurons in adults.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
located_in cytosol
inferred from direct assay
located_in nucleus
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CkIIα in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
EMBL-EBI Single Cell Expression Atlas
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 11 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 23 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of CkIIα
Transgenic constructs containing regulatory region of CkIIα
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
    Alleles Representing Disease-Implicated Variants
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Sterility
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (4)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    15 of 15
    Yes
    Yes
    1  
    7 of 15
    No
    Yes
    1  
    5 of 15
    No
    Yes
    1 of 15
    No
    No
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (2)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    13 of 15
    Yes
    Yes
    8 of 15
    No
    Yes
    Rattus norvegicus (Norway rat) (2)
    13 of 13
    Yes
    Yes
    6 of 13
    No
    Yes
    Xenopus tropicalis (Western clawed frog) (2)
    8 of 12
    Yes
    Yes
    4 of 12
    No
    Yes
    Danio rerio (Zebrafish) (5)
    13 of 15
    Yes
    Yes
    13 of 15
    Yes
    Yes
    10 of 15
    No
    Yes
    8 of 15
    No
    Yes
    7 of 15
    No
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (2)
    15 of 15
    Yes
    Yes
    1 of 15
    No
    No
    Arabidopsis thaliana (thale-cress) (4)
    9 of 9
    Yes
    Yes
    8 of 9
    No
    Yes
    8 of 9
    No
    Yes
    8 of 9
    No
    Yes
    Saccharomyces cerevisiae (Brewer's yeast) (2)
    10 of 15
    Yes
    Yes
    10 of 15
    Yes
    Yes
    Schizosaccharomyces pombe (Fission yeast) (1)
    11 of 12
    Yes
    Yes
    Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190B0Y )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506U2 )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Musca domestica
    House fly
    Glossina morsitans
    Tsetse fly
    Lucilia cuprina
    Australian sheep blowfly
    Mayetiola destructor
    Hessian fly
    Aedes aegypti
    Yellow fever mosquito
    Anopheles darlingi
    American malaria mosquito
    Anopheles gambiae
    Malaria mosquito
    Culex quinquefasciatus
    Southern house mosquito
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03XN )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Bombyx mori
    Silkmoth
    Danaus plexippus
    Monarch butterfly
    Apis florea
    Little honeybee
    Apis mellifera
    Western honey bee
    Bombus impatiens
    Common eastern bumble bee
    Bombus terrestris
    Buff-tailed bumblebee
    Linepithema humile
    Argentine ant
    Megachile rotundata
    Alfalfa leafcutting bee
    Nasonia vitripennis
    Parasitic wasp
    Dendroctonus ponderosae
    Mountain pine beetle
    Dendroctonus ponderosae
    Mountain pine beetle
    Tribolium castaneum
    Red flour beetle
    Pediculus humanus
    Human body louse
    Rhodnius prolixus
    Kissing bug
    Rhodnius prolixus
    Kissing bug
    Rhodnius prolixus
    Kissing bug
    Cimex lectularius
    Bed bug
    Cimex lectularius
    Bed bug
    Acyrthosiphon pisum
    Pea aphid
    Zootermopsis nevadensis
    Nevada dampwood termite
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03UN )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Ixodes scapularis
    Black-legged tick
    Stegodyphus mimosarum
    African social velvet spider
    Tetranychus urticae
    Two-spotted spider mite
    Daphnia pulex
    Water flea
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0AZY )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Strongylocentrotus purpuratus
    Purple sea urchin
    Ciona intestinalis
    Vase tunicate
    Gallus gallus
    Domestic chicken
    Gallus gallus
    Domestic chicken
    Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 2 )
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 2 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser

      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Subunit Structure (UniProtKB)
      Tetramer of two alpha and two beta chains.
      (UniProt, P08181 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Positive Regulators of Hedgehog Signaling Pathway -
      Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
      Metabolic Pathways
      External Data
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3L
      Recombination map
      3-47
      Cytogenetic map
      Sequence location
      3L:23,097,072..23,103,460 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      80D1-80D1
      Limits computationally determined from genome sequence between P{lacW}l(3)j1B10j1B10 and P{lacW}l(3)j2C4j2C4
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      80A-80A
      (determined by in situ hybridisation)
      Experimentally Determined Recombination Data
      Location
      Notes
      Stocks and Reagents
      Stocks (16)
      Genomic Clones (11)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (123)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of

      Source for merge of: CkIIα Tik

      Source for merge of: CkIIα anon-WO02059370.53

      Source for merge of: CkIIα l(3)S095808

      Additional comments

      Complementation tests and recombinational mapping suggest the CkIIα mutant maps to a novel circadian rhythm gene.

      Source for merge of CkIIα anon-WO02059370.53 was sequence comparison ( date:051113 ).

      Drosophila Casein kinase II α and β subunits can rescue the lethality of S.cerevisiae deleted for both genes.

      Other Comments

      When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G2/M phase cells, a whole range of mitotic abnormalities, centrosome abnormalities and lagging chromatids are seen.

      CkIIα may regulate eye morphogenesis via phosphorylation of E(spl).

      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

      A dominant mutant, which has a long period phenotype as a heterozygote. Homozygotes die during the third larval instar stage. The recessive lethal is not separable from the circadian phenotype, suggesting a vital role for the CkIIα gene.

      Identification: screen for circadian locomotor behavior mutants.

      In vivo activity of Antp is modified by CkIIα-mediated phosphorylation. Phosphorylation of Antp by CkIIα is important for preventing inappropriate activities of this homeotic protein during embryogenesis.

      Casein kinase II specifically phosphorylates a set of serine residues within the cact PEST domain.

      Casein kinase II phosphorylates Ser468 (a residue in the PEST domain) of cact in vitro.

      Casein kinase II (encoded by CkIIα and CkIIβ) phosphorylates dsh in vitro. This phosphorylation is regulated by fz2, implicating a role for Casein kinase II in wg signalling.

      Recombinant CkII in vitro binds to spermine and this interaction is concomitant with a striking effect on the structural polymeric organisation on the kinase which in the presence of the polyamine exhibits a ring-like structure.

      Casein kinase II reconstituted from heterologous subunits displays properties similar to those of the homologous protein.

      CkIIβ is primarily an inhibitor of the catalytic activity of CkIIα in the absence of salt.

      Casein kinase II-mediated phosphorylation of Top2 stimulates its activity by enhancing the ability of the enzyme to hydrolyse its high energy ATP cofactor.

      S.cerevisiae Scer\cka1 Scer\cka2 double mutants are functionally complemented by CkIIα.

      Isolation and sequencing of cDNA clones encoding both subunits of casein kinase II.

      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 65 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      EMBL-EBI Single Cell Expression Atlas
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Synonyms and Secondary IDs (52)
      Reported As
      Symbol Synonym
      CK2 alpha
      Cask-II-a
      anon-WO02059370.53
      dCKII alpha
      l(3)S095808
      Name Synonyms
      Casein kinase II alpha subunit
      Casein kinase II α subunit
      Casein kinase II-α subunit
      NAP1-kinase
      casein kinase II
      casein kinase II α
      casein kinase IIα
      lethal (3) S095808
      protein kinase CK2
      α subunit of Drosophila casein kinase II
      Secondary FlyBase IDs
      • FBgn0000258
      • FBgn0062248
      • FBgn0066006
      • FBgn0060573
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (216)