Open Close
General Information
Symbol
Dmel\CkIIα
Species
D. melanogaster
Name
casein kinase IIα
Annotation Symbol
CG17520
Feature Type
FlyBase ID
FBgn0264492
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As

CK2, CKII, CK2α, Tik, CKIIa

Key Links
Genomic Location
Cytogenetic map
Sequence location
3L:23,097,072..23,103,460 [+]
Recombination map

3-47

RefSeq locus
NT_037436 REGION:23097072..23103460
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. (P08181)
Summaries
Gene Group (FlyBase)
CMGC KINASES (UNCLASSIFIED) -
CMGC family members are primarily proline-directed serine/threonine kinases. (Adapted from FBrf0132098).
Pathway (FlyBase)
Positive Regulators of Hedgehog Signaling Pathway -
Positive regulators of hedgehog signaling down-regulation the pathway, enhancing the activation of transcription of hh-responsive genes.
Protein Function (UniProtKB)
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha chain contains the catalytic site. May participate in Wnt signaling.
(UniProt, P08181)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
CkII α: Casein kinase II, α subunit
The structural gene for the catalytic subunit of casein kinase II (CKII). CKII is a cyclic-nucleotide-independent, Ca2+ and calmodulin-insensitive protein kinase. It phosphorylates serine and threonine residues of a broad range of nuclear and non-nuclear proteins with functions in development, cell division, and oncogenesis. It exists as a 130,000 molecular weight α2β2 tetramer. Purification of the Drosophila enzyme described by Glover, Shelton, and Brutlag (1983, J. Biol. Chem. 258: 3258-65).
Gene Model and Products
Number of Transcripts
7
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\CkIIα or the JBrowse view of Dmel\CkIIα for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0070042
1536
336
FBtr0070043
1561
336
FBtr0070044
1423
336
FBtr0111141
1581
336
FBtr0344974
2053
336
FBtr0345138
1481
336
FBtr0345139
1429
336
Additional Transcript Data and Comments
Reported size (kB)

1.467 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0070041
40.0
336
7.26
FBpp0070042
40.0
336
7.26
FBpp0070043
40.0
336
7.26
FBpp0110433
40.0
336
7.26
FBpp0311229
40.0
336
7.26
FBpp0311359
40.0
336
7.26
FBpp0311360
40.0
336
7.26
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

Additional Polypeptide Data and Comments
Reported size (kDa)

336 (aa); 39.833 (kD predicted)

36.7 (kD observed)

Comments

Purified CkII protein kinases were able to phosphorylate acid-soluble proteins from Drosophila Kc cells.

External Data
Subunit Structure (UniProtKB)

Tetramer of two alpha and two beta chains.

(UniProt, P08181)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CkIIα using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (24 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN001171790
(assigned by GO_Central )
Biological Process (17 terms)
Terms Based on Experimental Evidence (14 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001171790
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001171790
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001171790
(assigned by GO_Central )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000621946
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000621946
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000621946
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
s-LNv neuron

Comment: 3 hours after ZT

mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

CkIIα is expressed in Pdf- and Clk-positive s-LNv neurons in adults.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CkIIα in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 11 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 23 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of CkIIα
Transgenic constructs containing regulatory region of CkIIα
Deletions and Duplications ( 0 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
 
7 of 15
No
Yes
 
5 of 15
No
Yes
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
8 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
13 of 13
Yes
Yes
6 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
8 of 12
Yes
Yes
4 of 12
No
Yes
Danio rerio (Zebrafish) (5)
13 of 15
Yes
Yes
13 of 15
Yes
Yes
10 of 15
No
Yes
8 of 15
No
Yes
7 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
15 of 15
Yes
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (4)
9 of 9
Yes
Yes
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
10 of 15
Yes
Yes
10 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
11 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190B0Y )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506U2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W03XN )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03UN )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0AZY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 2 )
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 2 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Tetramer of two alpha and two beta chains.
    (UniProt, P08181 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Positive Regulators of Hedgehog Signaling Pathway -
    Positive regulators of hedgehog signaling down-regulation the pathway, enhancing the activation of transcription of hh-responsive genes.
    Metabolic Pathways
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3L
    Recombination map

    3-47

    Cytogenetic map
    Sequence location
    3L:23,097,072..23,103,460 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    80D1-80D1
    Limits computationally determined from genome sequence between P{lacW}l(3)j1B10j1B10 and P{lacW}l(3)j2C4j2C4
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    80A-80A
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Notes
    Stocks and Reagents
    Stocks (16)
    Genomic Clones (11)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (123)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of

    Source for merge of: CkIIα Tik

    Source for merge of: CkIIα anon-WO02059370.53

    Source for merge of: CkIIα l(3)S095808

    Additional comments

    Complementation tests and recombinational mapping suggest the CkIIα mutant maps to a novel circadian rhythm gene.

    Source for merge of CkIIα anon-WO02059370.53 was sequence comparison ( date:051113 ).

    Drosophila Casein kinase II α and β subunits can rescue the lethality of S.cerevisiae deleted for both genes.

    Other Comments

    When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G2/M phase cells, a whole range of mitotic abnormalities, centrosome abnormalities and lagging chromatids are seen.

    CkIIα may regulate eye morphogenesis via phosphorylation of E(spl).

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    A dominant mutant, which has a long period phenotype as a heterozygote. Homozygotes die during the third larval instar stage. The recessive lethal is not separable from the circadian phenotype, suggesting a vital role for the CkIIα gene.

    Identification: screen for circadian locomotor behavior mutants.

    In vivo activity of Antp is modified by CkIIα-mediated phosphorylation. Phosphorylation of Antp by CkIIα is important for preventing inappropriate activities of this homeotic protein during embryogenesis.

    Casein kinase II specifically phosphorylates a set of serine residues within the cact PEST domain.

    Casein kinase II phosphorylates Ser468 (a residue in the PEST domain) of cact in vitro.

    Casein kinase II (encoded by CkIIα and CkIIβ) phosphorylates dsh in vitro. This phosphorylation is regulated by fz2, implicating a role for Casein kinase II in wg signalling.

    Recombinant CkII in vitro binds to spermine and this interaction is concomitant with a striking effect on the structural polymeric organisation on the kinase which in the presence of the polyamine exhibits a ring-like structure.

    Casein kinase II reconstituted from heterologous subunits displays properties similar to those of the homologous protein.

    CkIIβ is primarily an inhibitor of the catalytic activity of CkIIα in the absence of salt.

    Casein kinase II-mediated phosphorylation of Top2 stimulates its activity by enhancing the ability of the enzyme to hydrolyse its high energy ATP cofactor.

    S.cerevisiae Scer\cka1 Scer\cka2 double mutants are functionally complemented by CkIIα.

    Isolation and sequencing of cDNA clones encoding both subunits of casein kinase II.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 62 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Synonyms and Secondary IDs (52)
    Reported As
    Symbol Synonym
    CK2 alpha
    Cask-II-a
    anon-WO02059370.53
    dCKII alpha
    l(3)S095808
    Name Synonyms
    Casein kinase II alpha subunit
    Casein kinase II α subunit
    Casein kinase II-α subunit
    NAP1-kinase
    casein kinase II
    casein kinase II α
    casein kinase IIα
    lethal (3) S095808
    protein kinase CK2
    α subunit of Drosophila casein kinase II
    Secondary FlyBase IDs
    • FBgn0000258
    • FBgn0062248
    • FBgn0066006
    • FBgn0060573
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (211)