Ca2+/calmodulin-dependent protein kinase II, dCaMKII, CaM kinase II, l(4)16, CaM kinase
Please see the GBrowse view of Dmel\CaMKII or the JBrowse view of Dmel\CaMKII for information on other features
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Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.46
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Evidence supports alternative transcription start site within intronic TE (RAMPAGE TSS data, FBrf0220331; short-capped RNA data, FBrf0209722; RNA-Seq junction data). May be specific to sequenced strain; not included in gene model.
530, 516, 509, 490 (aa); 60, 58, 55 (kD)
490 (aa); 55 (kD)
There are four forms of CaMKII kinase which differ by insertions or deletions near the carboxy terminus of the putative link segment which is thought to join the amino- and carboxy-terminal globules. They were purified from transfected mammalian cells and Drosophila head extracts and their catalytic properties described. Catalytic activity is dependent on Ca2+ and calmodulin before autophosphorylation. Ca2+-independent activity is observed after autophosphorylation.
There are four forms of CaMKII kinase which differ by insertions or deletions near the carboxy terminus of the putative link segment which is thought to join the amino and carboxy-terminal globules. They were purified from transfected mammalian cells and Drosophila head extracts and their catalytic properties described. Catalytic activity is dependent on Ca2+ and calmodulin before autophosphorylation. Ca2+-independent activity is observed after autophosphorylation.
There are four forms of CaMKII kinase which differ by insertions or deletions near the carboxy terminus of the putative link segment which is thought to join the amino and carboxy-terminal globules. They were purified from transfected mammalian cells and Drosophila head extracts and their catalytic properties described. Catalytic activity is dependent on Ca2+ and calmodulin before autophosphorylation. Ca2+-independent activity is observed after autophosphorylation. The 516aa isoform is a minor form in adult heads.
One of several products generated by alternative splicing.
CaMKII protein purified 200-fold from adult Drosophila heads was shown to have kinase activity and to aquire Ca2+-independent activity after autophosphorylaton. CaMKII protein expressed from a Drosophila cDNA in COS cells was shown to have the same properties. Four autophosphorylation sites are conserved between the rat and the Drosophila enzymes.
Interacts with CASK.
Autophosphorylation at Thr-287 is independent of autophosphorylation at Thr-306 and Thr-307.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CaMKII using the Feature Mapper tool.
Comment: rapidly degraded
CaMKII transcripts from very early embryos have only the short 3' UTR form while adult heads have both the long and short 3' UTR forms.
CaMkII protein is expressed uniformly in body wall muscles at 14 hrs AEL. By 22 hrs AEL, CaMKII can be faintly detected in the axons and terminal buttons of motor neurons innervating the A1-7 ventral longitudinal muscles 1-4 (muscles 6, 7, 12 and 13). By 22 hrs AEL, CamKII immunoreactivity intensely labels the motor neuron terminal buttons, and the corresponding postsynaptic densities.
CaMKII protein is expressed at much greater levels in adult head than body.
Comment: strong expression
Comment: strong expression
Comment: faint expression
GBrowse - Visual display of RNA-Seq signals
View Dmel\CaMKII in GBrowse 24-0
4-0
4-1
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
monoclonal
Source for identity of: CaMKII CG18069
Source for merge of: CaMKII l(4)102EFb
Host gene for maternally inherited stable intronic sequence RNA (sisRNA).
Candidate stable intronic sequence RNA (sisRNA) identified within CDS of this gene.
Nonsense-mediated mRNA decay (NMD) down-regulates a distinct splice isoform(s) of this gene.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
The CaMKII protein is the kinase that phosphorylates phosrestin 1, the product of the Arr2 gene. The earliest light induced phosphorylation in the photoreceptor is the phosphorylation of a single site of Arr2 by a kinase dependent on both Ca2+ and calmodulin. The kinase that is responsible for the phosphorylation of Arr2 is CaMKII. Results suggest CaMKII may play a role in regulating light adaptation in photoreceptors.
The basic catalytic functions of six CaMKII isoforms are characterised. Results reveal the protein family is functionally homologous to the rat CaM kinase II.
Isoforms of CaMKII have similar biochemical properties, they exhibit differential activity by mutant Cam proteins. Data supports a role for the C-terminal variable region of CaMKII in the mechanism of activation and demonstrate that stimulation of CaMKII catalytic activity by Cam is a multistep process, with separate binding and activation steps.
A key regulator of plasticity in synaptic physiology and behaviour, alterations in its activity produce pleiotropic effects that involve synaptic transmission and development as well as various aspects of behaviour.
A fifth form of CaMKII is isolated, it is maternally derived and is identical to the 530 amino acid polypeptide except for the missing exon 11.
Study of transformed lines expressing a specific inhibitor of CaMKII (Rat\CamKII-I) suggests an important role of CaMKII in the stability of synaptic transmission.
CaMKII is alternatively spliced, producing a variety of protein isoforms.
A large gene with at least 16 exons encoding 4 forms of Cam kinase by alternate splicing.
