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General Information
Symbol
Dmel\Hph
Species
D. melanogaster
Name
HIF prolyl hydroxylase
Annotation Symbol
CG44015
Feature Type
FlyBase ID
FBgn0264785
Gene Model Status
Stock Availability
Gene Summary
HIF prolyl hydroxylase (Hph) encodes the prolyl-4 hydroxylase that hydroxylases proline 850a of the product of sima, which is the alpha-subunit of the hypoxia inducible factor (HIF). The product of Hph regulates growth through both independent and dependent mechanisms of the product of sima. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
Also Known As

fatiga, fga, l(3)02255, PHD

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
3-47.5
RefSeq locus
NT_033777 REGION:5257037..5269507
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN004393218
(assigned by GO_Central )
inferred from sequence model
inferred from electronic annotation with InterPro:IPR006620
(assigned by InterPro )
inferred from sequence or structural similarity with HGNC:1232,HGNC:14660,HGNC:14661
inferred from biological aspect of ancestor with PANTHER:PTN004393218
(assigned by GO_Central )
Biological Process (8 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from direct assay
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from high throughput mutant phenotype
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN004393218
(assigned by GO_Central )
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in nucleoplasm
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Summaries
Gene Snapshot
HIF prolyl hydroxylase (Hph) encodes the prolyl-4 hydroxylase that hydroxylases proline 850a of the product of sima, which is the alpha-subunit of the hypoxia inducible factor (HIF). The product of Hph regulates growth through both independent and dependent mechanisms of the product of sima. [Date last reviewed: 2019-03-07]
Gene Group (FlyBase)
PEPTIDYL-PROLINE DIOXYGENASES -
Peptidyl-proline dioxygenases catalyze the hydroxylation of peptidyl L-proline to peptidyl hydroxy-L-proline, where 2-oxoglutarate and one other act as hydrogen or electron donors with incorporation of one atom of oxygen into each donor.
Summary (Interactive Fly)

oxygen sensor - a specific prolyl-4-hydroxylases required for hydroxylation of key prolyl residues in the HIFα sequence - Fe (II) and 2-oxoglutarate dependent dioxygenase superfamily protein that uses O as a co-substrate for catalysis - involved in tracheal development, oogenesis and drives cell growth downstream of Cyclin D

Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\Hph for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q8SX21)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0334275
2337
325
FBtr0334274
1607
278
FBtr0334276
2496
478
FBtr0344333
3171
478
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0306390
36.3
325
6.87
FBpp0306389
30.6
278
6.67
FBpp0306391
52.6
478
8.01
FBpp0310716
52.6
478
8.01
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

478 aa isoforms: Hph-PC, Hph-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hph using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

The Hph-RA and Hph-RB transcripts are detected at all stages of development by RT-PCR but only Hph-RB is significantly upregulated in adults. Hph-RB but not Hph-RA is induced by hypoxia.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in nucleoplasm
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Hph in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 34 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Hph
Transgenic constructs containing regulatory region of Hph
Aberrations (Deficiencies and Duplications) ( 7 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
1  
11 of 15
No
Yes
9 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
11 of 15
No
Yes
8 of 15
No
Yes
Rattus norvegicus (Norway rat) (4)
9 of 13
Yes
Yes
8 of 13
No
Yes
7 of 13
No
Yes
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
10 of 12
Yes
Yes
10 of 12
Yes
Yes
5 of 12
No
Yes
Danio rerio (Zebrafish) (4)
12 of 15
Yes
Yes
11 of 15
No
Yes
9 of 15
No
Yes
7 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
10 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    RNA-RNA
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-47.5
    Cytogenetic map
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    82F7-82F8
    Limits computationally determined from genome sequence between P{PZ}l(3)0273302733 and P{EP}EP974
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Location based on deficiency mapping with Df(3R)3-4.
    82F4-82F7
    (determined by in situ hybridisation)
    82F3-82F6
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (18)
    Genomic Clones (31)
    cDNA Clones (29)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: Hph CG1114

    Source for database merge of

    Source for merge of: CG1114 CG14665

    Source for merge of: Hph l(3)02255

    Source for merge of: Hph anon- EST:Posey69

    Source for merge of: Hph l(3)S030304

    Source for merge of: Hph l(3)82Fe

    Source for merge of: Hph CR31541

    Additional comments

    l(3)L3051 may correspond to Hph: the P{lacW}l(3)L3051L3051 insertion maps within the transcription unit.

    Annotations CG31543 and CR31541 merged as CG44015 in release 5.47 of the genome annotation; CR31541 corresponds to alternative 3' UTR of CG31543. Merge supported by RNA-seq junction data.

    P{PZ}Hph02255 is inserted upstream of Hph.

    Annotations CG1114, CG14665 merged as CG31543 in release 3 of the genome annotation.

    Other Comments

    sima protein nuclear localization is affected in Hph mutant embryos.

    Renamed annotation (CR31541) in release 3 of the genome annotation.

    A mutation in Hph causes major defects in embryonic tracheal development.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 71 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    MARRVEL_MODEL - MARRVEL (model organism gene)
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (32)
    Reported As
    Symbol Synonym
    CG2676
    CR31541
    Dm.egl-9
    anon-EST:Posey69
    l(3)82Fe
    l(3)S030304
    Secondary FlyBase IDs
    • FBgn0264652
    • FBgn0086689
    • FBgn0028167
    • FBgn0051543
    • FBgn0037306
    • FBgn0027674
    • FBgn0010773
    • FBgn0037308
    • FBgn0013577
    • FBgn0037309
    Datasets (8)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    Study result (8)
    Result
    Result Type
    Title
    Clustering analysis of hemocytes from non-infested third instar larvae
    Clustering analysis of hemocytes from wasp-infested third instar larvae
    Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
    Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
    Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
    Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
    Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
    Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
    References (111)