FlyBase Gene Report: Dmel\Hph

General Information

Symbol

Dmel\Hph

Species

D. melanogaster

Name

HIF prolyl hydroxylase

Annotation Symbol

CG44015

Feature Type

protein_coding_gene

FlyBase ID

FBgn0264785

Gene Model Status

Stock Availability

18 publicly available

Gene Snapshot

HIF prolyl hydroxylase (Hph) encodes the prolyl-4 hydroxylase that hydroxylases proline 850a of the product of sima, which is the alpha-subunit of the hypoxia inducible factor (HIF). The product of Hph regulates growth through both independent and dependent mechanisms of the product of sima. [Date last reviewed: 2019-03-07]

Other Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

fatiga, fga, l(3)02255, PHD

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:5,257,037..5,269,507 [+]

Recombination map

3-47.5

RefSeq locus

NT_033777 REGION:5257037..5269507

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables peptidyl-proline dioxygenase activity

inferred from direct assay

(Arquier et al., 2006)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables ferrous iron binding

inferred from biological aspect of ancestor with PANTHER:PTN004393218

(assigned by GO_Central )

(Gaudet et al., 2011)

enables iron ion binding

inferred from sequence model

(Bruick and McKnight, 2001)

enables L-ascorbic acid binding

inferred from electronic annotation with InterPro:IPR006620

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables peptidyl-proline 4-dioxygenase activity

inferred from sequence or structural similarity with HGNC:1232,HGNC:14660,HGNC:14661

(Bruick and McKnight, 2001)

enables peptidyl-proline dioxygenase activity

inferred from biological aspect of ancestor with PANTHER:PTN004393218

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (9 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in cellular response to hypoxia

inferred from mutant phenotype

(Bruick and McKnight, 2001)

involved_in peptidyl-proline hydroxylation

inferred from direct assay

(Arquier et al., 2006)

involved_in positive regulation of cell growth

inferred from mutant phenotype

(Centanin et al., 2005)

inferred from genetic interaction with FLYBASE:CycD; FB:FBgn0010315, FLYBASE:Cdk4; FB:FBgn0016131

(Frei and Edgar, 2004)

involved_in protein export from nucleus

inferred from mutant phenotype

(Irisarri et al., 2009)

involved_in protein localization

inferred from mutant phenotype

(Lavista-Llanos et al., 2002)

involved_in response to hypoxia

inferred from mutant phenotype

(Lavista-Llanos et al., 2002)

involved_in terminal branching, open tracheal system

inferred from high throughput mutant phenotype

(Ghabrial et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in cellular response to hypoxia

inferred from biological aspect of ancestor with PANTHER:PTN004393218

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in oxidation-reduction process

inferred from electronic annotation with InterPro:IPR005123, InterPro:IPR006620

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

involved_in peptidyl-proline hydroxylation to 4-hydroxy-L-proline

inferred from sequence or structural similarity with HGNC:1232,HGNC:14660,HGNC:14661

(Bruick and McKnight, 2001)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleoplasm

inferred from direct assay

(Frei and Edgar, 2004)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

PEPTIDYL-PROLINE DIOXYGENASES

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

PEPTIDYL-PROLINE DIOXYGENASES -: Peptidyl-proline dioxygenases catalyze the hydroxylation of peptidyl L-proline to peptidyl hydroxy-L-proline, where 2-oxoglutarate and one other act as hydrogen or electron donors with incorporation of one atom of oxygen into each donor.

Summary (Interactive Fly)

oxygen sensor - a specific prolyl-4-hydroxylases required for hydroxylation of key prolyl residues in the HIFα sequence - Fe (II) and 2-oxoglutarate dependent dioxygenase superfamily protein that uses O as a co-substrate for catalysis - involved in tracheal development, oogenesis and drives cell growth downstream of Cyclin D

Interactive Fly

Gene Model and Products

Number of Transcripts

4

Number of Unique Polypeptides

3

Please see the JBrowse view of Dmel\Hph for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

(FlyBase Genome Annotators, 2012)

protein_coding_gene

(FlyBase Genome Annotators, 2012)

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0334275

2337

325

FBtr0334274

1607

278

FBtr0334276

2496

478

FBtr0344333

3171

478

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0306390

36.3

325

6.87

FBpp0306389

30.6

278

6.67

FBpp0306391

52.6

478

8.01

FBpp0310716

52.6

478

8.01

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

478 aa isoforms: Hph-PC, Hph-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hph using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Hph_1
Hph_2

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage -- embryonic stage

(Acevedo et al., 2010)

Additional Descriptive Data

The Hph-RA and Hph-RB transcripts are detected at all stages of development by RT-PCR but only Hph-RB is significantly upregulated in adults. Hph-RB but not Hph-RA is induced by hypoxia.

(Acevedo et al., 2010)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleoplasm

inferred from direct assay

(Frei and Edgar, 2004)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Hph in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Hph

View exonic expression by tissue for Dmel\Hph

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0264785

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31543

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 34 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Hph

Transgenic constructs containing regulatory region of Hph

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation

Gene disrupted in

(Ghabrial et al., 2011, Lavista-Llanos et al., 2002, Carpenter, 1999, Carpenter, 1994)

(Carpenter, 1999, Szeged Stock Center, 1998-, Carpenter, 1994)

(Roote, 2004.7.20, Ryder and Roote, 2004.11.10)

(Roote, 2004.7.20, Ryder and Roote, 2004.11.10)

(Carpenter, 1999, Carpenter, 1994)

Gene not disrupted in

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

(Carpenter, 1999, Carpenter, 1994)

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(3R)3-4)

Hph⁰²²⁵⁵

lethal | larval stage

Hph⁰²²⁵⁵

lethal | recessive

lethal - all die before end of first instar larval stage

lethal - all die before end of first instar larval stage | recessive

Hph¹

lethal - all die before end of pupal stage

Hph⁹

partially lethal - majority die (with Hph⁰²²⁵⁵)

Hph^S030304

partially lethal - majority die (with Hph^S030304)

Hph⁰²²⁵⁵

partially lethal - majority die | embryonic stage

Hph⁰²²⁵⁵

partially lethal - majority die | embryonic stage (with Df(3R)3-4)

Hph⁰²²⁵⁵

partially lethal - majority die | larval stage (with Df(3R)3-4)

Hph⁰²²⁵⁵

Sterility

Allele

Other Phenotypes

Allele

abnormal cell growth | somatic clone, with Scer\GAL4^Act5C.PP

Hph^UAS.cFa

abnormal cell migration, with Scer\GAL4^slbo.2.6

Hph^UAS.cFa

abnormal developmental rate | larval stage | recessive

Hph⁹

abnormal size | somatic clone, with Scer\GAL4^Act5C.PP

decreased body size | adult stage (with Hph⁰²²⁵⁵)

Hph^S030304

decreased body size | adult stage (with Hph^S030304)

Hph⁰²²⁵⁵

decreased body size | pupal stage | recessive

Hph⁹

decreased cell size | somatic clone | wandering third instar larval stage

Hph⁰²²⁵⁵

increased cell number, with Scer\GAL4^en-e16E

Hph^EP3200

some die during first instar larval stage

some die during first instar larval stage | recessive

Hph¹

some die during pupal stage

Hph⁹

some die during pupal stage (with Hph⁰²²⁵⁵)

Hph^S030304

some die during pupal stage (with Hph^S030304)

Hph⁰²²⁵⁵

Phenotype manifest in

Allele

border follicle cell, with Scer\GAL4^slbo.2.6

Hph^UAS.cFa

embryonic/larval fat body | somatic clone

Hph⁰²²⁵⁵

embryonic/larval tracheal system

eye | somatic clone

Hph⁷⁸⁴

terminal tracheal cell

Hph⁷⁸⁴

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

12 of 15

Yes

Yes

1

11 of 15

No

Yes

9 of 15

No

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

12 of 15

Yes

Yes

11 of 15

No

Yes

8 of 15

No

Yes

Rattus norvegicus (Norway rat) (4)

9 of 13

Yes

Yes

8 of 13

No

Yes

7 of 13

No

Yes

2 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (3)

10 of 12

Yes

Yes

10 of 12

Yes

Yes

5 of 12

No

Yes

Danio rerio (Zebrafish) (4)

12 of 15

Yes

Yes

11 of 15

No

Yes

9 of 15

No

Yes

7 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

10 of 15

Yes

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190920 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00010952

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503CB )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY011572

Glossina morsitans

Tsetse fly

Gmor\GMOY002109

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10656

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11860

Mayetiola destructor

Hessian fly

Mdes\Mdes006766

Mayetiola destructor

Hessian fly

Mdes\Mdes000900

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002798

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002802

Anopheles darlingi

American malaria mosquito

Adar\ADAC009832

Anopheles darlingi

American malaria mosquito

Adar\ADAC009844

Anopheles gambiae

Malaria mosquito

Agam\AGAP003523

Anopheles gambiae

Malaria mosquito

Agam\AGAP003524

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008669

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008670

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008668

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0340 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA006496

Bombyx mori

Silkmoth

Bmor\BGIBMGA005121

Bombyx mori

Silkmoth

Bmor\BGIBMGA005120

Danaus plexippus

Monarch butterfly

Dple\DPOGS214589-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS211014-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL006042

Heliconius melpomene

Postman butterfly

Hmel\HMEL012180

Apis florea

Little honeybee

Aflo\2933981252

Apis florea

Little honeybee

Aflo\2271393892

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Bombus terrestris

Buff-tailed bumblebee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002f3f

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0015db

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002d85

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008788

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG011018

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM515220

Pediculus humanus

Human body louse

Phum\PHUM362800

Rhodnius prolixus

Kissing bug

Rpro\RPRC000503

Cimex lectularius

Bed bug

Clec\CLEC005933

Cimex lectularius

Bed bug

Clec\CLEC005932

Cimex lectularius

Bed bug

Clec\CLEC008104

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009943

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11972

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_14224

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X030V )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR003018

Strigamia maritima

European centipede

Smar\SMAR002384

Strigamia maritima

European centipede

Smar\SMAR003057

Ixodes scapularis

Black-legged tick

Isca\ISCW000901

Stegodyphus mimosarum

African social velvet spider

Smim\KFM80564.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur23g01880

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_347567

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_64722

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_347569

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G03SP )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Ciona intestinalis

Vase tunicate

Cint\LOC100180463

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005376

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000019760

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000004104

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

EGLN1; egl-9 family hypoxia inducible factor 1

model of familial erythrocytosis 3

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EGLN3; egl-9 family hypoxia inducible factor 3

12 of 15

EGLN1; egl-9 family hypoxia inducible factor 1

11 of 15

familial erythrocytosis 3

EGLN2; egl-9 family hypoxia inducible factor 2

9 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

luminiscence technology, necessary binding region

(De Lella Ezcurra et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Frei et al., 2005)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Frei and Edgar, 2004)

suppressible

(Doronkin et al., 2010)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264785

MIST (protein-protein) - An integrated Molecular Interaction Database

Hph

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3R

Recombination map

3-47.5

Cytogenetic map

Sequence location

3R:5,257,037..5,269,507 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

82F7-82F8

Limits computationally determined from genome sequence between P{PZ}l(3)02733⁰²⁷³³ and P{EP}EP974

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

82F-82F

(Ghabrial et al., 2011)

Location based on deficiency mapping with Df(3R)3-4.

(Ghabrial et al., 2011)

82F4-82F7

(determined by in situ hybridisation)

(Spradling et al., 1999)

82F3-82F6

(determined by in situ hybridisation)

(Szeged Stock Center, 1998-, Deak et al., 1997)

82F4-82F7

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-47.5

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (18)

20142

y¹ w^67c23; P{EPgy2}Hph^EY02317

86580

w¹¹¹⁸; PBac{RB}Hph^e04483/TM6B, Tb¹

18714

w¹¹¹⁸; PBac{WH}Hph^f03923 mRpL13^f04195

113179

w^*; P{GawB}Hph^NP3244

123022

w¹¹¹⁸; P{RS3}Hph^CB-0049-3

123266

w¹¹¹⁸; P{RS3}Hph^CB-0635-3

125054

w¹¹¹⁸; P{RS5}Hph^5-HA-1078

125340

w¹¹¹⁸; P{RS5}Hph^5-HA-1681

125519

w¹¹¹⁸; P{RS5}Hph^5-HA-2003

104278

w^*; P{GawB}Hph^NP2662 / TM3, Sb¹ Ser¹

v103382

P{KK100344}VIE-260B

v318655

PBac{fTRG00761.sfGFP-TVPTBF}VK00002

More stocks available...

Genomic Clones (31)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (29)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0264785

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40633

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Frei and Edgar, 2004)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Hph CG1114

(Bruick and McKnight, 2001)

Source for database merge of

Source for merge of: CG1114 CG14665

(Taylor, 2001)

Source for merge of: Hph l(3)02255

(Lavista-Llanos et al., 2002)

Source for merge of: Hph anon- EST:Posey69

(Ashburner, 2004.6.30)

Source for merge of: Hph l(3)S030304

(Frei and Edgar, 2004)

Source for merge of: Hph l(3)82Fe

(Ghabrial et al., 2011)

Source for merge of: Hph CR31541

(FlyBase Genome Annotators, 2012)

Additional comments

l(3)L3051 may correspond to Hph: the P{lacW}l(3)L3051^L3051 insertion maps within the transcription unit.

(Gene Disruption Project members, 2015.10.8)

Annotations CG31543 and CR31541 merged as CG44015 in release 5.47 of the genome annotation; CR31541 corresponds to alternative 3' UTR of CG31543. Merge supported by RNA-seq junction data.

(FlyBase Genome Annotators, 2012)

P{PZ}Hph⁰²²⁵⁵ is inserted upstream of Hph.

(Frei and Edgar, 2003)

Annotations CG1114, CG14665 merged as CG31543 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Other Comments

sima protein nuclear localization is affected in Hph mutant embryos.

(Irisarri et al., 2009)

Renamed annotation (CR31541) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

A mutation in Hph causes major defects in embryonic tracheal development.

(Wappner et al., 2002)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 63 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40633

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0264785

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0264785

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Hph_1
Hph_2

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31543

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40633

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC033631

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG44015

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264785

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

Hph

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha

Synonyms and Secondary IDs (32)

Reported As

Symbol Synonym

0303/04

(Deak et al., 1997)

31543

(Ravi et al., 2009)

CG1114

(Zimmermann et al., 2006, Lavista-Llanos et al., 2002, Bruick and McKnight, 2001)

CG14665

(Veraksa et al., 2002)

CG2676

CG31543

(Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Cuttell et al., 2008, Ashburner, 2004.6.30)

CG44015

(Meadows, 2019.11.20, FlyBase Genome Annotators, 2012)

CR31541

Dm.egl-9

(Taylor, 2001)

Fatiga

(Barretto et al., 2020)

Fga

(Chen et al., 2019)

FgaB

(Perez-Perri et al., 2016)

PHD

(Dong et al., 2015, Rytkönen et al., 2011)

anon-EST:Posey69

dHPH

(Cuttell et al., 2008)

dPHD

(Arquier et al., 2006)

dPHD2

(van Uden et al., 2011)

dmHPH

(Bruick and McKnight, 2001)

fatiga

(De Lella Ezcurra et al., 2016)

fga

(Bertolin et al., 2016, De Lella Ezcurra et al., 2016, Zhou and Haddad, 2013, Acevedo et al., 2010, Irisarri et al., 2009, Centanin et al., 2008, Acevedo et al., 2007, Centanin et al., 2005, Centanin et al., 2004, Centanin et al., 2003)

hph

(Mazina et al., 2020, Dong et al., 2015, Doronkin et al., 2010, Parrish et al., 2009, Datar et al., 2006, Duronio, 2004)

l(3)02255

(French et al., 2003, Spradling et al., 1999, BDGP Project Members, 1994-1999)

l(3)82Fe

l(3)S030304

Name Synonyms

Fatiga

(Chen et al., 2019, Bandarra et al., 2015, Mandilaras et al., 2013, van Uden et al., 2011, Frei et al., 2008, Chen et al., 2007)

HIF prolyl 4-hydroxylase

(Myllharju and Kivirikko, 2004)

HIF prolyl hydroxylase

(Doronkin et al., 2010, Bruick and McKnight, 2001)

HIF-1a prolyl hyroxylase

(Wappner et al., 2002)

lethal (3) 82Fe

lethal (3) S030304

lopped

(Ghabrial et al., 2011)

prolyl-4-hydroxylase

(Rytkönen et al., 2011)

Secondary FlyBase IDs

FBgn0264652
FBgn0086689
FBgn0028167
FBgn0051543
FBgn0037306
FBgn0027674
FBgn0010773
FBgn0037308
FBgn0013577
FBgn0037309

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (107)