α-adaptin, alpha-Adaptin, α-Ada, ada, AP2
Please see the GBrowse view of Dmel\AP-2α or the JBrowse view of Dmel\AP-2α for information on other features
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Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
5.1, 4.3 (northern blot)
6.3, 4.8 (northern blot)
None of the polypeptides share 100% sequence identity.
939 (aa); 105 (kD predicted)
940 (aa)
Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type and beta-type subunits), a medium adaptin (mu-type subunit AP50) and a small adaptin (sigma-type subunit AP17).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\AP-2α using the Feature Mapper tool.
Comment: maternally deposited
Comment: rapidly degraded
Comment: reported as ventral nerve cord anlage
Protein is localized to the cell cortex and in the cytoplasm of interphase sensory organ precursor cells. However, beginning in metaphase and throughout mitosis the protein becomes assymetrically localized to the cell cortex near one centrosome and after cytokinesis is partitioned into the pIIb cell that also contains the numb protein.
α-Adaptin proteinis detected in embryonic extracts by Western blots. A detailed descriptionof it\'s distribution in embryos as determined by immunolocalization isprovided. In summary, during cellularization, it is seen between and aheadof the elongating nuclei and redistributes to the cell surface duringgastrulation. In later stages, it is expressed in a complex and dynamicpattern. Strong sites of expression include neuroblasts, presumptivestomatogastric nervous sytem, lateral chordotonal organs, garland cells,adult midgut precursors, antenno-maxillary complex, endoderm, fat bodies,and the visceral mesoderm. In larvae, it is localized to the plasmamembrane in the synaptic boutons of the neuromuscular junctions. Itappears to be a good marker for endocytic activity.
GBrowse - Visual display of RNA-Seq signals
View Dmel\AP-2α in GBrowse 22-0.7
Maps to 2L.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: α-Adaptin CG4260
Source for identity of: AP-2α α-Adaptin
Source for merge of: α-Adaptin MENE(2L)-A
Source for merge of: α-Adaptin l(2)SH0460
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
2 alleles of l(2)SH0460 recovered in a P-insertion screen.
Homozygous clones induced during early larval development do not survive. Clones induced late in larval development do survive but at a growth disadvantage compared to wild type twin spots.
An α-Adaptin cDNA has been cloned and sequenced, and its expression pattern has been analysed.
In presynaptic terminals α-Adaptin defines a network-like membrane structure to which the GTPase dynamin is recruited. α-Adaptin is necessary for the formation of clathrin coated pits and participates in the dynamin-dependent release of coated vesicles from the membrane surface. Results suggest an α-Adaptin-dependent control of the vesicle cycle that maintains the balance between the amount of vesicle- and surface-associated membranes.
