FlyBase Gene Report: Dmel\RapGAP1

General Information

Symbol

Dmel\RapGAP1

Species

D. melanogaster

Name

Rap GTPase activating protein 1

Annotation Symbol

CG44086

Feature Type

protein_coding_gene

FlyBase ID

FBgn0264895

Gene Model Status

Current

Stock Availability

26 publicly available

Gene Snapshot

Rap GTPase activating protein 1 (RapGAP1) encodes a small G protein that mediates the activation of ERK signaling via the kinase encoded by tor. [Date last reviewed: 2019-08-01]

Other Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

RapGAP

Key Links

Genomic Location

Cytogenetic map

28B1-28A6

Sequence location

2L:7,497,805..7,578,980 [-]

Recombination map

2-27

RefSeq locus

NT_033779 REGION:7497805..7578980

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables GTPase activator activity

inferred from direct assay

(Chen et al., 1997)

inferred from mutant phenotype

(Mishra et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables GTPase activator activity

inferred from biological aspect of ancestor with PANTHER:PTN000403785

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in negative regulation of small GTPase mediated signal transduction

inferred from direct assay

(Chen et al., 1997)

inferred from mutant phenotype

(Mishra et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in activation of GTPase activity

inferred from biological aspect of ancestor with PANTHER:PTN000403785

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in germ plasm

inferred from direct assay

(Chen et al., 1997)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000403785

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

RAP-LIKE GTPASE ACTIVATING PROTEINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

RAP-LIKE GTPASE ACTIVATING PROTEINS -: Rap-like GTPase activating proteins (RapGAP) are an evolutionary-related group of RasGAPs that promote GTP hydrolysis by either Ral, Rap or Rheb subfamilies of the Ras GTPases. (Adapted from FBrf0229300).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RapGAP1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.47

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RapGAP1-RD

FBtr0334846

NM_144299

4545

933

RapGAP1-RE

FBtr0334847

NM_001014478

4951

850

RapGAP1-RF

FBtr0334848

NM_001014479

4222

876

RapGAP1-RH

FBtr0334849

NM_001103644

4521

959

RapGAP1-RI

FBtr0334850

NM_001273279

4462

933

Additional Transcript Data and Comments

Reported size (kB)

4.5 (northern blot)

(Chen et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

RapGAP1-PD

FBpp0306870

101.3

933

7.19

NP_652556

AAF52528

RapGAP1-PE

FBpp0306871

92.9

850

7.12

NP_001014478

AAF52526

RapGAP1-PF

FBpp0306872

96.1

876

7.39

NP_001014479

AAX52662

RapGAP1-PH

FBpp0306873

104.9

959

7.41

NP_001097114

ABV53642

RapGAP1-PI

FBpp0306874

101.3

933

7.19

NP_001260208

AGB92744

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

933 aa isoforms: RapGAP1-PD, RapGAP1-PI

Additional Polypeptide Data and Comments

Reported size (kDa)

850 (aa); 94 (kD predicted)

(Chen et al., 1997)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RapGAP1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

pole plasm

Comment: maternally deposited

(Fisher et al., 2012)

organism

• pole plasm

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

anterior endoderm

(Fisher et al., 2012)

germline cell

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

garland cell primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval garland cell

(Fisher et al., 2012)

embryonic/larval lymph gland

(Fisher et al., 2012)

embryonic stage 4 -- 5

cephalic furrow

(Wang et al., 2013)

ventral furrow

(Wang et al., 2013)

embryonic/larval posterior midgut

(Wang et al., 2013)

dorsal fold | posterior

(Wang et al., 2013)

Additional Descriptive Data

During cellularization, the zygotic transcription of RapGAP1 displays a complex pattern that consists of four distinct, spatially restricted expression domains, overlapping the presumptive region of the cephalic furrow, the ventral furrow, the posterior midgut, and the posterior dorsal fold, respectively. This last expression domain centers on the initiating cells of the posterior fold and encompasses both the anterior and posterior flanking regions. Thus, across the dorsal epithelium, the levels of RapGAP1 zygotic transcription are high in the region surrounding the posterior fold but low in the cells near the anterior fold.

(Wang et al., 2013)

Rapgap1 transcripts are first detected in the posterior pole of the embryo. They are observed in the pole cells as they form. They are detected in pole cells until they migrate through the midgut wall. During germ band extension, they are found in groups of cells in the neurectoderm. After germ band retraction, they are detected in cells of the PNS, in the garland cells and in the gut. In larvae, Rapgap1 transcripts are observed in imaginal discs. In the eye disc, they are observed in most cells of the morphogenetic furrow. Immediate y posterior to the furrow, expression is rapidly restricted to the developing ommatidial clusters.

(Chen et al., 1997)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval optic lobe

(Chen et al., 1997)

optic nerve

• axon

(Chen et al., 1997)

eye disc

(Chen et al., 1997)

morphogenetic furrow

(Chen et al., 1997)

ommatidial precursor cluster

(Chen et al., 1997)

eye disc posterior to the morphogenetic furrow

(Chen et al., 1997)

embryonic stage 13 -- 17

garland cell

(Chen et al., 1997)

presumptive embryonic/larval peripheral nervous system

(Chen et al., 1997)

embryonic/larval midgut

(Chen et al., 1997)

embryonic stage 8 -- 11

ventral neurectoderm

(Chen et al., 1997)

embryonic stage 5

primordial germ cell

(Chen et al., 1997)

third instar larval stage

embryonic/larval central nervous system

(Sanyal, 2009)

embryonic/larval optic lobe

(Sanyal, 2009)

eye disc

(Sanyal, 2009)

synaptic neuropil

(Sanyal, 2009)

motor neuron

(Sanyal, 2009)

Additional Descriptive Data

Strong expression is observed in the larval central nervous system but not in motor neuron axons or body wall sensor neurons. Strong expression is also seen in the eye disc and optic lobes. Strong expression is also observed in the larval brain neuropil, both in the ventral nerve cord and in the mushroom body neuropil in the brain. Expression is additionally seen in the soma of dorsal medial motor neurons.

(Sanyal, 2009)

Rapgap1 protein is first detected in the posterior pole of the embryo in the pole cells as they form. It is detected in pole cells until they migrate through the midgut wall. During germ band extension, Rapgap1 protein is found in groups of cells in the neurectoderm. After germ band retraction, it is detected in cells of the PNS, in the garland cells and in the gut. In larvae, Rapgap1 protein is observed in imaginal discs. In the eye disc, it is observed in most cells of the morphogenetic furrow. Immediately posterior to the furrow expression is rapidly restricted to the developing ommatidial clusters. It is also detected in the axons of the optic nerve and in the optic lobe.

(Chen et al., 1997)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in germ plasm

inferred from direct assay

(Chen et al., 1997)

Expression Deduced from Reporters

Reporter: P{GawB}cb20

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 17

embryonic/larval midgut longitudinal visceral muscle

(Ward et al., 2002)

third instar larval stage

embryonic/larval central nervous system

(Ward et al., 2002)

embryonic/larval brain

(Ward et al., 2002)

embryonic/larval mushroom body

(Ward et al., 2002)

larval enteroendocrine cell | subset

(Lajeunesse et al., 2010)

embryonic/larval proventriculus | restricted

(Lajeunesse et al., 2010)

embryonic/larval midgut | restricted

(Lajeunesse et al., 2010)

embryonic/larval proventriculus

(Lajeunesse et al., 2010)

oogenesis & adult stage | female

terminal filament

(Ward et al., 2002)

female germline stem cell

(Ward et al., 2002)

oogenesis stage S14 & adult stage | female

dorsal appendage forming follicle cell | faint

(Ward et al., 2002)

oogenesis stage S1 -- S10 & adult stage | female

nurse cell | subset

(Ward et al., 2002)

adult stage | male

testis | intense

(Ward et al., 2002)

Reporter: P{GawB}OK6

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- pupal stage

trachea | restricted

(Aberle et al., 2002)

salivary gland

(Aberle et al., 2002)

wing disc

(Aberle et al., 2002)

motor neuron

(Aberle et al., 2002)

motor neuron

Comment: expression assayed in cultured cells

(Kuppers-Munther et al., 2004)

larval stage

apterous-expressing neuron of the lateral cluster of the thorax Ap4 | subset

(Marques et al., 2003)

motor neuron

(McCabe et al., 2004, Marques et al., 2003, Marques et al., 2002)

larval CCAP A1-A4 EN abdominal neuron

(Veverytsa and Allan, 2011)

embryonic stage

motor neuron

(Landgraf et al., 2003, McCabe et al., 2003)

third instar larval stage

ventral nerve cord

(Sanyal, 2009)

embryonic/larval brain

(Sanyal, 2009)

motor neuron

(Sanyal, 2009)

embryonic/larval optic lobe

(Sanyal, 2009)

RP2 motor neuron

(Sanyal, 2009)

aCC neuron

(Sanyal, 2009)

synaptic neuropil

(Sanyal, 2009)

A1-7 dorsal motor neuron

(Sanyal, 2009)

Reporter: P{ST.lexA::HG}RapGAP1^SE422

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval central nervous system

(Bloomington Drosophila Stock Center, 2019)

ventral nerve cord

(Bloomington Drosophila Stock Center, 2019)

Reporter: P{ST.lexA::HG}RapGAP1^VF-4

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

wing disc

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval central nervous system

(Bloomington Drosophila Stock Center, 2019)

ventral nerve cord

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval salivary gland

(Bloomington Drosophila Stock Center, 2019)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RapGAP1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RapGAP1

View exonic expression by tissue for Dmel\RapGAP1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0264895

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 41 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RapGAP1

Transgenic constructs containing regulatory region of RapGAP1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation

Gene disrupted in

Df(2L)Exel6018

(Ryder, 2004.4.26)

Inferred from location

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^da.Switch.PT

RapGAP1^GX5503-1

viable

RapGAP1^e04634

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable (with Df(2L)ED479)

RapGAP1^k10609

Sterility

Allele

fertile

RapGAP1^e04634

Other Phenotypes

Allele

abnormal behavior | recessive

RapGAP1^BG01433

visible

RapGAP1^GMR.PC

visible, with Scer\GAL4^pnr-MD237

RapGAP1^GD12000

Phenotype manifest in

Allele

mesothoracic tergum, with Scer\GAL4^pnr-MD237

RapGAP1^GD12000

pigment cell

RapGAP1^GMR.PC

trichogen cell, with Scer\GAL4^pnr-MD237

RapGAP1^GD12000

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RAP1GAP

12 of 15

Yes

Hsap\RAP1GAP2

11 of 15

Yes

Hsap\GARNL3

3 of 15

Yes

Hsap\SIPA1L3

3 of 15

Yes

Hsap\SIPA1

2 of 15

Yes

Hsap\SIPA1L1

2 of 15

Yes

Hsap\SIPA1L2

2 of 15

Yes

Hsap\RALGAPA1

1 of 15

Hsap\RALGAPA2

1 of 15

Hsap\TSC2

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rap1gap

12 of 15

Yes

Mmus\Rap1gap2

11 of 15

Yes

Mmus\Garnl3

2 of 15

Yes

Mmus\Sipa1l1

2 of 15

Yes

Mmus\Sipa1l2

2 of 15

Yes

Mmus\Sipa1l3

2 of 15

Yes

Mmus\Sipa1

2 of 15

Yes

Mmus\Ralgapa1

1 of 15

Mmus\Ralgapa2

1 of 15

Mmus\Tsc2

1 of 15

Rattus norvegicus (Norway rat) (8)

Rnor\Rap1gap

10 of 13

Yes

Rnor\Rap1gap2

7 of 13

Yes

Rnor\Garnl3

2 of 13

Yes

Rnor\Sipa1l2

2 of 13

Yes

Rnor\Sipa1l3

2 of 13

Yes

Rnor\Sipa1

2 of 13

Yes

Rnor\Ralgapa2

1 of 13

Rnor\Sipa1l1

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (7)

Xtro\rap1gap2

5 of 12

Yes

Xtro\garnl3

3 of 12

Yes

Xtro\rap1gap

2 of 12

Yes

Xtro\sipa1l1

2 of 12

Yes

Xtro\cacna1d

1 of 12

Xtro\ralgapa1

1 of 12

Xtro\tsc2

1 of 12

Danio rerio (Zebrafish) (14)

Drer\rap1gap2a

11 of 15

Yes

Drer\si:dkey-166d12.2

9 of 15

Yes

Drer\rap1gap

7 of 15

Yes

Drer\rap1gap2b

6 of 15

Yes

Drer\si:ch1073-90m23.1

5 of 15

Yes

Drer\si:ch211-182d3.1

3 of 15

Yes

Drer\LOC101884255

2 of 15

Yes

Drer\sipa1l1

2 of 15

Yes

Drer\sipa1l2

2 of 15

Yes

Drer\sipa1l3

2 of 15

Yes

Drer\zmp:0000001168

2 of 15

Yes

Drer\ralgapa1

1 of 15

Drer\ralgapa2

1 of 15

Drer\sipa1l2

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\F53A10.2

13 of 15

Yes

Cele\sipa-1

2 of 15

Cele\hgap-1

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\tsc2

1 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902PD )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25836

Drosophila persimilis

Dper\GL18868

Drosophila willistoni

Dwil\GK24374

Drosophila virilis

Dvir\GJ23610

Drosophila mojavensis

Dmoj\GI23099

Drosophila grimshawi

Dgri\GH10582

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150227 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17022281

Glossina morsitans

Tsetse fly

Gmor\GMOY008157

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_14377

Mayetiola destructor

Hessian fly

Mdes\Mdes011020

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL005998

Anopheles darlingi

American malaria mosquito

Adar\ADAC010068

Anopheles gambiae

Malaria mosquito

Agam\AGAP009533

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018142

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01HZ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA008858

Danaus plexippus

Monarch butterfly

Dple\DPOGS208456-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL017902

Apis florea

Little honeybee

Aflo\2147553379

Apis mellifera

Western honey bee

Amel\Rapgap1

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP24230

Bombus terrestris

Buff-tailed bumblebee

Bter\15202536

Linepithema humile

Argentine ant

Lhum\LH17239

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0009ad

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008207

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_12109

Tribolium castaneum

Red flour beetle

Tcas\TC034036

Pediculus humanus

Human body louse

Phum\PHUM268890

Rhodnius prolixus

Kissing bug

Rpro\RPRC007445

Cimex lectularius

Bed bug

Clec\CLEC001956

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008129

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11849

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01G5 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR013476

Ixodes scapularis

Black-legged tick

Isca\ISCW017798

Stegodyphus mimosarum

African social velvet spider

Smim\KFM74753.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_300644

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_257870

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G028T )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Rap1gap

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000005868

Gallus gallus

Domestic chicken

Ggal\RAP1GAP

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (3)

Gene Symbol

Score

Source

Alignment

rad

4 of 10

CG5521

1 of 10

gig

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SIPA1L3; signal induced proliferation associated 1 like 3

model of cataract 45

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RAP1GAP; RAP1 GTPase activating protein

12 of 15

600278

RAP1GAP2; RAP1 GTPase activating protein 2

11 of 15

618714

GARNL3; GTPase activating Rap/RanGAP domain like 3

3 of 15

SIPA1L3; signal induced proliferation associated 1 like 3

3 of 15

616655

CATARACT 45; CTRCT45

cataract 45

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RapGAP1 - Gαo

two hybrid

(Kopein and Katanaev, 2009)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

RapGAP1

enhanceable

Rap1

(Chen et al., 1997)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

pbl

enhanceable

RapGAP1

(Gregory et al., 2007)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264895

MIST (protein-protein) - An integrated Molecular Interaction Database

RapGAP1

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Rap1 signalling

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-27

Cytogenetic map

28B1-28A6

Sequence location

2L:7,497,805..7,578,980 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

28B1-28A6

Limits computationally determined from genome sequence between P{EP}EP2419^EP2419&P{EP}EP575 and P{PZ}l(2)rL220^rL220; Limits computationally determined from genome sequence between P{PZ}cuc¹⁰⁶⁰⁷ and P{EP}EP2419^EP2419&P{EP}EP575

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

28B-28B

(Chen, 1997.9.9)

Experimentally Determined Recombination Data

Location

2-27

(FlyBase, 2016)

2-24.4

(Comeron et al., 2012)

(Chen, 1997.9.9)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (26)

Bloomington

12749

w¹¹¹⁸; P{GT1}RapGAP1^BG02277

31250

y¹ v¹; P{TRiP.JF01766}attP2

42782

y¹ v¹; P{TRiP.GL01152}attP2

18279

w¹¹¹⁸; PBac{RB}RapGAP1^e04634

66663

w^*; P{ST.lexA::HG}RapGAP1^VF-4

FlyORF

F002433

M{UAS-RapGAP1.ORF.3xHA.GW}ZH-86Fb

v101846

v102659

v106358

v26721

w¹¹¹⁸; P{GD12000}v26721

v46781

w¹¹¹⁸; P{GD16772}v46781

v46782

w¹¹¹⁸; P{GD16772}v46782/CyO

More stocks available...

Genomic Clones (71)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (83)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0264895

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34032

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Chen et al., 1997)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Rapgap1 CG6682

(Deng, 1999.11)

Source for identity of: RapGAP1 Rapgap1

(FlyBase, 2009-)

Source for database merge of

Source for merge of: Rapgap1 CG13790

(FlyBase Genome Annotators, 2007)

Source for merge of: Rapgap1 CG13789

(FlyBase Genome Annotators, 2005)

Source for merge of: Rapgap1 CG13791

(FlyBase Genome Annotators, 2012)

Additional comments

Annotations CG34374 and CG13791 merged as CG44086 in release 5.47 of the genome annotation. Merge supported by RNA-seq junction and expression data.

(FlyBase Genome Annotators, 2012)

FlyBase curator comment: 'Rapgap1' changed to 'RapGAP1' to capitalize the 'GAP' to be consistent with all other GAP gene symbols.

(FlyBase, 2009-)

Annotations CG33529 and CG13790 merged as CG34374 in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

Annotations CG13789 and CG6682 merged as CG33529 (which corresponds to Rapgap1) in release 4.1 of the genome annotation.

(FlyBase Genome Annotators, 2005)

"CG13791" may correspond to "Scim14". Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places "Scim14" near/in "CG13791".

(Dobie and Karpen, 2001.4.18)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Regulation of Rapgap1 mRNA and protein localisation by posterior group genes during the formation of pole plasm is studied.

(Chen et al., 1997)

Rapgap1 gene product is a GTPase activating protein.

(Chen et al., 1995)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 54 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34032

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0264895

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0264895

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34032

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034036

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG44086

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264895

MIST (protein-protein) - An integrated Molecular Interaction Database

RapGAP1

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Rap1 signalling

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

CG13789

CG13790

(Cole et al., 2012.7.6, Nicholson et al., 2008, Gregory et al., 2007, Zhang et al., 2007, Manak et al., 2006)

CG13791

(Cole et al., 2012.7.6, Reis et al., 2010, Dubruille et al., 2009, Edwards et al., 2009, Keleman et al., 2009.8.5, Nicholson et al., 2008, Zhang et al., 2007)

CG33529

(Aerts et al., 2010, Macdonald and Long, 2006)

CG34374

(Hosono et al., 2015, Keleman et al., 2009.8.5, Ni et al., 2008, FlyBase Genome Annotators, 2007)

CG44086

(FlyBase Genome Annotators, 2012, Ni et al., 2010.12.1)

CG6682

FBpp0111515

(van Dam et al., 2011)

Q9VM02_DROME

(Fukuyama et al., 2012)

RapGAP

(Hall et al., 2019, Gilmour et al., 2017)

RapGAP1

(Kockel et al., 2019, Fochler et al., 2017, Blick et al., 2016, Clandinin and Owens, 2016-, Gene Disruption Project members, 2016-, Kockel et al., 2016)

RapGap1

(Bakal et al., 2007)

Rapgap1

(Sawant et al., 2018, Bonneaud et al., 2017, Duff et al., 2015, Liu and Lasko, 2015, Wang et al., 2013, Webber et al., 2013, Lloret-Llinares et al., 2012, Paik et al., 2012, Cherbas et al., 2011, Diez et al., 2011, Aerts et al., 2010, Foley et al., 2010, Saja et al., 2010, Dubruille et al., 2009, Sanyal, 2009, Kwon et al., 2008, Bakal et al., 2007, Manak et al., 2006, Burnette et al., 2005)

rapgap

(Blick et al., 2016)

rapgap1

(Rangan et al., 2009, Rangan et al., 2008)

Name Synonyms

CG13790

Rap GTPase activating protein 1

Rapgap1

(Kopein and Katanaev, 2009)

Secondary FlyBase IDs

FBgn0085403
FBgn0031922
FBgn0014015
FBgn0053529
FBgn0040955
FBtr0079452
FBpp0079080
FBtr0079453
FBpp0079081
FBtr0079454
FBpp0079082
FBgn0031923

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (100)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections