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Gene Dmel\Inr-a

General Information
Symbol	Dmel\Inr-a	Species	D. melanogaster
Name	Inverse regulator a	Annotation symbol	CG10228
Feature type	protein_coding_gene	FlyBase ID	FBgn0264962
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	Pcf11, l(2)k08015
Genomic Location
Chromosome (arm)	2R	Recombination map	2-[59]
Cytogenetic map	44C1-50B9	Sequence location	2R:10,748,661..10,758,576 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Inverse regulator a is referred to in FlyBase by the symbol Dmel\Inr-a (CG10228, FBgn0264962). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA binding. There is experimental evidence that it is involved in the biological process: neurogenesis. 16 alleles are reported. The phenotypes of these alleles are annotated with: mesothoracic tergum; crystal cell; pigment cell. It has 6 annotated transcripts and 6 annotated polypeptides. Protein features are: CID domain; ENTH/VHS; RNA polymerase II-binding domain. Gene sequence location is 2R:10748661..10758576.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 44C1-50B9   Left limit from inclusion within Tp(2;Y)G44 (FBrf0054158) Right limit from inclusion within Tp(2;Y)G44 (FBrf0054158)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 51D3-51D5   (determined by in situ hybridisation)   (Spradling et al., 1999) 51D3-51D5     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-[59]
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Inr-a for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Inr-a-RB FBtr0301513  NM_001169682   2647   573 Inr-a-RC FBtr0302109  NM_001169683   819   86 Inr-a-RD FBtr0302294  NM_137155   6321   1953 Inr-a-RE FBtr0302560  NM_001202002   5979   1839 Inr-a-RF FBtr0339985     6012   1850 Inr-a-RG FBtr0339986     6297   1945
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Inr-a-PB   FBpp0290728   63.3   573   10.70     NP_001163153   ACZ94425 Inr-a-PC   FBpp0291319   10.2   86   5.68     NP_001163154   ACZ94426 Inr-a-PD   FBpp0291500   212.8   1953   6.14     NP_610999   AAF58192 Inr-a-PE   FBpp0291716   200.9   1839   6.07     NP_001188931   ADV37177 Inr-a-PF   FBpp0309006   201.9   1850   6.03       Inr-a-PG   FBpp0309007   211.9   1945   6.22
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • CID domain (IPR006569) ENTH/VHS (IPR008942) RNA polymerase II-binding domain (IPR006903)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263680   AA540187   AA567123   AA567896   AA698671   AA817665   AA942456   AA950180   AA978927   AE013599 AAF58192   AE013599 ACZ94425   AE013599 ACZ94426   AE013599 ADV37177   AI455434   AI455910   AI456805   AQ025854   AX094022   AY075387   AY231688   BG638769   BI356805   BI637966   BI640721   BP552362   BP552363   BP560122   BT001647 AAN71402   BT064645   BT088974   BT099611   BT133501   CK605000   CO188515   CO188730   CO190024   CO265445   CO265637   CO267802   CO268449   CO270645   CO272495   CO272733   CO283101   CO287679   CO293696   CO294095   CO301148   CO308272   CO319251   CO321059   CO325812   CO331221   CO333822   CO342228   CO343232   EC066902   EC068346   EC071850   EC084074   EC086749   EC197142   EC197330   EC199646   EC200238   EC204287   EC204387   EC206718   EC206763   EC210614   EC218906   EC221698   EC238187   EC241316   EC244682   EC255554   EC256151   EL869910   EW712640   FE426491   FE426575   FE862718   FE862988   FE863177   FE863208   FE863273
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	A1Z9W4 E1JH68 C0P8M6 Q8IGR2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    24 central nervous system, pupae P8    18 head, virgin 1-day female    13 head, virgin 4-day female    15 head, virgin 20-day female    15 head, mated 1-day female    14 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    22 head, mated 20-day male    25 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    7 fat body, white prepupae    11 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    10 ovary, virgin 4-day female    20 ovary, mated 4-day female    17 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    24 central nervous system, pupae P8    18 head, virgin 1-day female    13 head, virgin 4-day female    15 head, virgin 20-day female    15 head, mated 1-day female    14 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    22 head, mated 20-day male    25 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    7 fat body, white prepupae    11 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    10 ovary, virgin 4-day female    20 ovary, mated 4-day female    17 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    24 central nervous system, pupae P8    18 head, virgin 1-day female    13 head, virgin 4-day female    15 head, virgin 20-day female    15 head, mated 1-day female    14 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    22 head, mated 20-day male    25 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    7 fat body, white prepupae    11 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    10 ovary, virgin 4-day female    20 ovary, mated 4-day female    17 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    24 central nervous system, pupae P8    18 head, virgin 1-day female    13 head, virgin 4-day female    15 head, virgin 20-day female    15 head, mated 1-day female    14 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    22 head, mated 20-day male    25 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    7 fat body, white prepupae    11 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    10 ovary, virgin 4-day female    20 ovary, mated 4-day female    17 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    24 central nervous system, pupae P8    18 head, virgin 1-day female    13 head, virgin 4-day female    15 head, virgin 20-day female    15 head, mated 1-day female    14 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    22 head, mated 20-day male    25 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    7 fat body, white prepupae    11 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    10 ovary, virgin 4-day female    20 ovary, mated 4-day female    17 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    24 central nervous system, pupae P8    18 head, virgin 1-day female    13 head, virgin 4-day female    15 head, virgin 20-day female    15 head, mated 1-day female    14 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    22 head, mated 20-day male    25 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    7 fat body, white prepupae    11 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    10 ovary, virgin 4-day female    20 ovary, mated 4-day female    17 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    24 central nervous system, pupae P8    18 head, virgin 1-day female    13 head, virgin 4-day female    15 head, virgin 20-day female    15 head, mated 1-day female    14 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    22 head, mated 20-day male    25 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    7 fat body, white prepupae    11 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    10 ovary, virgin 4-day female    20 ovary, mated 4-day female    17 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    24 central nervous system, pupae P8    18 head, virgin 1-day female    13 head, virgin 4-day female    15 head, virgin 20-day female    15 head, mated 1-day female    14 head, mated 4-day female    15 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    22 head, mated 20-day male    25 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    22 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    8 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    7 fat body, white prepupae    11 fat body, pupae P8    12 carcass, larvae L3 wandering    18 carcass, 1-day adult    10 carcass, 4-day adult    11 carcass, 20-day adult    10 ovary, virgin 4-day female    20 ovary, mated 4-day female    17 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    36 embryo 04-06hr    30 embryo 06-08hr    37 embryo 08-10hr    32 embryo 10-12hr    28 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    25 embryo 18-20hr    22 embryo 20-22hr    27 embryo 22-24hr    23 larva L1    13 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    18 white prepupae new    21 white prepupae 12hr    21 white prepupae 24hr    25 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    15 adult male 01day    13 adult male 05day    16 adult male 30day    17 adult female 01day    16 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  26 embryo 02-04hr  (36) embryo 04-06hr  (30) embryo 06-08hr  (37) embryo 08-10hr  (32) embryo 10-12hr  (28) embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    25 embryo 18-20hr    22 embryo 20-22hr  (27) embryo 22-24hr    23 larva L1    13 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    18 white prepupae new    21 white prepupae 12hr    21 white prepupae 24hr    25 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    15 adult male 01day    13 adult male 05day    16 adult male 30day    17 adult female 01day    16 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    36 embryo 04-06hr    30 embryo 06-08hr    37 embryo 08-10hr    32 embryo 10-12hr    28 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    25 embryo 18-20hr    22 embryo 20-22hr    27 embryo 22-24hr    23 larva L1    13 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    18 white prepupae new    21 white prepupae 12hr    21 white prepupae 24hr    25 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    15 adult male 01day    13 adult male 05day    16 adult male 30day    17 adult female 01day    16 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    36 embryo 04-06hr    30 embryo 06-08hr    37 embryo 08-10hr    32 embryo 10-12hr    28 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    25 embryo 18-20hr    22 embryo 20-22hr    27 embryo 22-24hr    23 larva L1    13 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    18 white prepupae new    21 white prepupae 12hr    21 white prepupae 24hr    25 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    15 adult male 01day    13 adult male 05day    16 adult male 30day    17 adult female 01day    16 adult female 05day    19 adult female 30day    19 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    36 embryo 04-06hr    30 embryo 06-08hr    37 embryo 08-10hr    32 embryo 10-12hr    28 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    25 embryo 18-20hr    22 embryo 20-22hr    27 embryo 22-24hr    23 larva L1    13 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    18 white prepupae new    21 white prepupae 12hr    21 white prepupae 24hr    25 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    15 adult male 01day    13 adult male 05day    16 adult male 30day    17 adult female 01day    16 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  26 embryo 02-04hr  (36) embryo 04-06hr  30 embryo 06-08hr  (37) embryo 08-10hr  32 embryo 10-12hr  28 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    25 embryo 18-20hr    22 embryo 20-22hr  27 embryo 22-24hr    23 larva L1    13 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    18 white prepupae new    21 white prepupae 12hr    21 white prepupae 24hr    25 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    15 adult male 01day    13 adult male 05day    16 adult male 30day    17 adult female 01day    16 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    36 embryo 04-06hr    30 embryo 06-08hr    37 embryo 08-10hr    32 embryo 10-12hr    28 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    25 embryo 18-20hr    22 embryo 20-22hr    27 embryo 22-24hr    23 larva L1    13 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    18 white prepupae new    21 white prepupae 12hr    21 white prepupae 24hr    25 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    15 adult male 01day    13 adult male 05day    16 adult male 30day    17 adult female 01day    16 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    26 embryo 02-04hr    36 embryo 04-06hr    30 embryo 06-08hr    37 embryo 08-10hr    32 embryo 10-12hr    28 embryo 12-14hr    25 embryo 14-16hr    25 embryo 16-18hr    25 embryo 18-20hr    22 embryo 20-22hr    27 embryo 22-24hr    23 larva L1    13 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    18 white prepupae new    21 white prepupae 12hr    21 white prepupae 24hr    25 pupae 2d postWPP    21 pupae 3d postWPP    13 pupae 4d postWPP    15 adult male 01day    13 adult male 05day    16 adult male 30day    17 adult female 01day    16 adult female 05day    19 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    15 embryonic 1182-4H    22 embryonic GM2    19 embryonic Kc167    12 embryonic S1    12 embryonic S3    24 leg disc CME L1    20 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    14 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    15 ovary fGS/OSS    30 ovary OSC    16 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    15 embryonic 1182-4H    22 embryonic GM2    19 embryonic Kc167    12 embryonic S1    12 embryonic S3    24 leg disc CME L1    20 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9  (33) wing disc ML-DmD16-c3    20 wing disc ML-DmD21    14 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2  (47) tumorous blood cells mbn2    15 ovary fGS/OSS  (30) ovary OSC    16 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    15 embryonic 1182-4H    22 embryonic GM2    19 embryonic Kc167    12 embryonic S1    12 embryonic S3    24 leg disc CME L1    20 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    14 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    15 ovary fGS/OSS    30 ovary OSC    16 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    15 embryonic 1182-4H    22 embryonic GM2    19 embryonic Kc167    12 embryonic S1    12 embryonic S3    24 leg disc CME L1    20 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    14 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    15 ovary fGS/OSS    30 ovary OSC    16 ovary OSS    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    15 embryonic 1182-4H    22 embryonic GM2    19 embryonic Kc167    12 embryonic S1    12 embryonic S3    24 leg disc CME L1    20 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    14 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    15 ovary fGS/OSS    30 ovary OSC    16 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    15 embryonic 1182-4H    22 embryonic GM2    19 embryonic Kc167    12 embryonic S1    12 embryonic S3    24 leg disc CME L1    20 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9  (33) wing disc ML-DmD16-c3    20 wing disc ML-DmD21    14 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2  (47) tumorous blood cells mbn2    15 ovary fGS/OSS  30 ovary OSC    16 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    15 embryonic 1182-4H    22 embryonic GM2    19 embryonic Kc167    12 embryonic S1    12 embryonic S3    24 leg disc CME L1    20 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    14 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    15 ovary fGS/OSS    30 ovary OSC    16 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    15 embryonic 1182-4H    22 embryonic GM2    19 embryonic Kc167    12 embryonic S1    12 embryonic S3    24 leg disc CME L1    20 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    20 wing disc ML-DmD21    14 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    20 antennal disc ML-DmD20-c5    8 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    47 tumorous blood cells mbn2    15 ovary fGS/OSS    30 ovary OSC    16 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    11 cold shock, 4-day adult    9 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    6 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Inr-aGD6907 lethal | larval stage | recessive Inr-aEMS1 Inr-aEMS2 Inr-ahd1 lethal | recessive Inr-aEMS2 Inr-ak08015 Inr-ak08023 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Inr-aGD6907 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 Inr-aGD6907 neuroanatomy defective | larval stage Inr-aGD6907 non-suppressor of variegation, with In(1)wm4h Inr-aEMS2 visible, with Scer\GAL4pnr-MD237 Inr-aGD6907 Phenotype manifest in Allele crystal cell Inr-ak08015 embryonic/larval neuroblast | larval stage Inr-aGD6907 ganglion mother cell | larval stage Inr-aGD6907 mesothoracic tergum, with Scer\GAL4pnr-MD237 Inr-aGD6907 pigment cell Inr-aEMS2
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of Inr-a Class Mutagen Stocks Known lesion Inr-ak08015 P-element activity 2 Yes Inr-ak08023 P-element activity 1 -- Inr-aEMS1 ethyl methanesulfonate 0 -- Inr-aEMS2 ethyl methanesulfonate 0 Yes Inr-aEMS3 ethyl methanesulfonate 0 -- Inr-aEMS4 ethyl methanesulfonate 0 -- Inr-aEMS5 ethyl methanesulfonate 0 -- Inr-ahd1 loss of function allele PM hybrid dysgenesis 0 Yes Inr-aγb gamma ray 0 -- Inr-aγc gamma ray 0 Yes Inr-aγd gamma ray 0 -- Inr-aγsc gamma ray 0 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Inr-a Class Mutagen Stocks Known lesion Inr-aGD6907 in vitro construct - RNAi in vitro construct - regulatory fusion 3 Yes Inr-aHMS00406 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Inr-aKK100722 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Inr-aNIG.10228R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)EP2590-Δ1 (Dworak et al., 2001) Df(2R)EP2590-Δ11 (Dworak et al., 2001) Df(2R)EP2590-Δ14 (Dworak et al., 2001) Df(2R)G44 (Rabinow et al., 1991) Df(2R)knSA3 (Sekelsky, 1999.9.21, Sekelsky et al., 2000) Df(2R)Pdel-1 (Xie and Birchler, 2012) Df(2R)Pdel-2 (Xie and Birchler, 2012)
Duplicated in	Dp(2;2)Pdup-3 (Xie and Birchler, 2012, Xie and Birchler, 2012) Dp(2;2)Pdup-4 (Xie and Birchler, 2012) Dp(2;Y)G44 (Rabinow et al., 1991)
Not disrupted in	Df(2R)Jp1 (Spradling et al., 1999, Sekelsky, 1999.9.21, Sekelsky et al., 2000, BDGP Project Members, 1994-1999) Df(2R)Jp4 (Sekelsky et al., 2000) Df(2R)XTE-18 (Sekelsky et al., 2000)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Inr-ak08015 No P{lacW}Inr-ak08023 No P{lacW}l(2)SH1522SH1522 No
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function
CV term Evidence References
RNA binding inferred from direct assay (Zhang and Gilmour, 2006)
Biological Process
CV term Evidence References
neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mRNA cleavage inferred from sequence or structural similarity with NCBI:NP_010514 (Mount and Salz, 2000)
Cellular Component
CV term Evidence References
mRNA cleavage factor complex inferred from sequence or structural similarity with NCBI:NP_010514 (Mount and Salz, 2000)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Inr-a allele Gene References Inr-aEMS2 w (Bhadra et al., 1998, Pal Bhadra et al., 1998) Inr-aβb w (Rabinow et al., 1991) Inr-aβc w (Rabinow et al., 1991) Inr-aβd w (Rabinow et al., 1991) Inr-aβsc w (Rabinow et al., 1991) unspecified w (Rabinow et al., 1991)
External Data
Linkouts
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG670V2R Dipteran inclusive ortholog search EOG6QNN49 Insect inclusive ortholog search EOG6S7HJZ Arthropod inclusive ortholog search EOG6D254S Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG670V2R)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Inr-a     Drosophila simulans   Dsim\GD25648 Y   Drosophila simulans   Dsim\GD15230     Drosophila sechellia   Dsec\GM20171 Y   Drosophila erecta   Dere\GG22388 Y   Drosophila yakuba   Dyak\GE12277 Y   Drosophila ananassae   Dana\GF13346     Drosophila ananassae   Dana\GF12082 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA24192 Y   Drosophila persimilis   Dper\GL17525 Y   Drosophila persimilis   Dper\GL22763     Drosophila willistoni   Dwil\GK22121 Y   Drosophila virilis   Dvir\GJ21559 Y   Drosophila mojavensis   Dmoj\GI18953 Y   Drosophila grimshawi   Dgri\GH22985 Y
Orthologs in non-Drosophila Dipterans (EOG6QNN49)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011663   Anopheles gambiae Malaria mosquito  Agam\AGAP001271   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ001186   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ001187
Orthologs in non-Dipteran Insects (EOG6S7HJZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB55641   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG014985   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG014982   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12616   Atta cephalotes Leafcutter ant  Acep\ACEP18617   Camponotus floridanus Florida carpenter ant  Cflo\CFLO13107   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14218   Linepithema humile Argentine ant  Lhum\LH15740   Pogonomyrmex barbatus Red harvester ant  Pbar\PB11524   Pogonomyrmex barbatus Red harvester ant  Pbar\PB14005   Solenopsis invicta Red fire ant  Sinv\SINV18794   Solenopsis invicta Red fire ant  Sinv\SINV18890   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004003   Bombyx mori Silkmoth  Bmor\BGIBMGA009372   Pediculus humanus Human body louse  Phum\PHUM005980   Tribolium castaneum Red flour beetle  Tcas\GLEAN_08263
Orthologs in non-Insect Arthropods (EOG6D254S)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_254414   Daphnia pulex Water flea  Dpux\COI   Ixodes scapularis Deer tick  Isca\ISCW009701   Ixodes scapularis Deer tick  Isca\ISCW009703   Ixodes scapularis Deer tick  Isca\ISCW009702
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD25648 Drosophila sechellia Dsec\GM20171 Drosophila erecta Dere\GG22388 Drosophila yakuba Dyak\GE12277 Drosophila ananassae Dana\GF12082 Drosophila pseudoobscura pseudoobscura Dpse\GA24192 Drosophila persimilis Dper\GL17525 Drosophila willistoni Dwil\GK22121 Drosophila virilis Dvir\GJ21559 Drosophila mojavensis Dmoj\GI18953 Drosophila grimshawi Dgri\GH22985
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	32411 y1 sc* v1; P{TRiP.HMS00406}attP2 10756 y1 w67c23; P{lacW}Inr-ak08015/CyO
Kyoto	102613 y1 w67c23 ; P{lacW}Inr-ak08023 / CyO 111233 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{neoFRT}42D P{lacW}Inr-ak08015 /CyO y+
VDRC	v103710 P{KK100722}VIE-260B v38365 w1118; P{GD6907}v38365 v38366 w1118; P{GD6907}v38366 v44115 w1118; P{GD6907}v44115
Genomic Clones ( 1 )
	BACR03K23 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 73 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD11480 LD24195 LD33071 RE43027
Other clones	FI20283 MIP05908
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM01150 HL01960 HL05452 LD06854 LD19585 LD26686 LD30123 LD33132 LD36938 SD20178 SD23706 UT01606
Other clones	8810 17514 31991 32689 38820 45868 57980 74413 153785 166111 172937 173622 214311.69 214569.60 CK01.50D.F1 CK01.51A.E8 CK01.57D.D5 CK01.83B.H6 CK01.117C.D8 CK01.119D.F4 CK01.128C.G1 EK040652 EK041721 EK046015 EK066463 EK091354 EK099625 EK107401 EK108157 EK142663 EK143250 EK151858 EK162244 EK180239 EK204143 EK206005 EK257260 EK277443 EK290231 EK298840 EK308107 EP02555 EP14209 EP16346 FGM218C12 FGM324G08 IP02052 LD35642 RM05183 RM05683 RM05983 RM06283 RM06383 RM06483 RM06583
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Pcf11 l(2)k08015 (Zhang and Gilmour, 2006)
Source for database merge of	Source for merge of: Inr-a Pcf11 (Xie and Birchler, 2012) Source for merge of: l(2)k08015 anon-WO0118547.146 (FlyBase, 1996-) Source for merge of: l(2)k08015 CG10228 (Mutsuddi et al., 2004)
Additional comments	Source for merge of l(2)k08015 anon-WO0118547.146 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	dsRNA made from templates generated with primers directed against this gene. (Zhang and Gilmour, 2006) Pcf11 inhibits transcription elongation by PolIIA under conditions that promote pausing. (Zhang and Gilmour, 2006) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) Area matching Drosophila EST AA978927. This EST has sequence similarity to human PCF11p homolgue. (Davies, 2001.3.15) The Inr-a regulatory gene interacts with the white locus via regulatory sequences. (Rabinow et al., 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_137155 NM_001169682 NM_001169683 NM_001202002 RefSeq (Proteins) • NP_610999 NP_001163153 NP_001163154 NP_001188931 DDBJ / EMBL / GenBank • AA817665 AA978927 AAF58192 AAN71402 ACZ94425 ACZ94426 ADV37177 AI455434 AQ025854 AX094022 AY075387 BI356805 BT001647 Entrez Gene - A searchable database of RefSeq genes. • 36658 UniProtKB/TrEMBL • A1Z9W4 C0P8M6 E1JH68 Q8IGR2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • CID domain (IPR006569) ENTH/VHS (IPR008942) RNA polymerase II-binding domain (IPR006903)
Linkouts
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	anon-WO0118547.146   CG10228 (Milchanowski et al., 2004, Mount and Salz, 2000, Keleman et al., 2009.8.5, Ni et al., 2010.12.1) dPcf11 (Zhang and Gilmour, 2006) Inr-a (Xie and Birchler, 2012, ) l(2)k08015 (Sekelsky et al., 2000, Dworak et al., 2001, FlyBase, 1996-, Mutsuddi et al., 2004, Spradling et al., 1999, Davies et al., 2001) Pcf11 (Xie and Birchler, 2012, Japanese National Institute of Genetics, 2012.5.21, Guo et al., 2011, Yao et al., 2007, Wasbrough et al., 2010, Neumüller et al., 2011) pcf11 (Zhou et al., 2008)
Name Synonym	Inverse regulator a   lethal (2) k08015   Pcf11
Secondary FlyBase IDs
	FBgn0004398 FBgn0022063 FBgn0033975 FBgn0062215
References ( 36 )
Generate a list of	All references relating to this gene ( 36 )
List References by type	Research paper  ( 16 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) Patent  ( 1 ) DNA/RNA sequence record  ( 1 )
Recent research papers ( 4 )
	Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Xie and Birchler, 2012, G3 (Bethesda) 2(6): 701--706 Identification of Inverse Regulator-a (Inr-a) as Synonymous with Pre-mRNA Cleavage Complex II Protein (Pcf11) in Drosophila. [FBrf0218562] Guo et al., 2011, Mol. Cell. Biol. 31(4): 639--651 Poly(A) Signals Located near the 5' End of Genes Are Silenced by a General Mechanism That Prevents Premature 3'-End Processing. [FBrf0212905] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...