ceb, central brain deranged, l(1)G0099, fs(1)M72, CT4318
Low-frequency RNA-Seq exon junction(s) not annotated.
Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.
Gene model reviewed during 5.46
Gene model reviewed during 5.55
Forms a complex with Nrx and Cont.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nrg using the Feature Mapper tool.
Comment: reported as dorsal/lateral sensory complexes
Nrg-XP is excluded from the calyx of the larval mushroom body, but not from the lobes.
The long Nrg protein isoform (designated NRG-180) is expressed throughout the third instar larval and adult brain. It is prominent in axons but is also present in cell bodies. In larval, pupal, and adult mushroom bodies, staining is observed in all lobes. High expression levels are observed in the adult gamma and alpha beta lobes. Expression is lower in axons of the alpha'beta' neurons.
Nrg protein is detected in the glial cell membranes in the peripheral nerves, ensheathing the axons and in the axons themselves. In the chordotonal organs, it is detected in the scolopale and cap cells, and in the scolopidial neuron.
Nrg protein expression is enriched in border follicle cells relative to other cells in the egg chamber in early stage 9.
In the early prepupal stages before head eversion, tThe neural-specific Nrg180 isoform is expressed by both ocellar pioneer and bristle mechanosensory neurons, but not by the external bristle cells.
Nrg is expressed at the lateral cell contacts of follicle cells in the ovary.
The shorter form of the Nrg protein is expressed in the epidermal cells of the imaginal discs. Since the antibody which detects the short form of Nrg also detects the long form, it is not clear how much of the signal detected is attributable to the short form. Developmental western blots show that the shorter Nrg polypeptide is first unambiguously detected in 8 hour embryos, and is most strongly expressed in 8-12 hour embryos.
The long form of Nrg protein is expressed on the surface of central and peripheral nervous system neurons, and on some PNS support cells. The long form is detected starting at 6 hours of embryogenesis, in one neuron per CNS hemisegment. At 7 hours, CNS expression extends to a 4-5 cell wide longitudinal stripe which prefigures the location of the longitudinal axon tracts. PNS expression is detected by 7 hours. Expression observed in larval imaginal discs is also restricted to neurons. Developmental western blots indicate that the peak of expression for the longer Nrg polypeptide is reached shortly after the peak for the shorter form.
A monoclonal antibody which recognizes both forms of the Nrg protein detects Nrg protein in neurons of the central nervous system and peripheral nervous system of the embryo, as well as in epidermal cells and neurons of third instar larval imaginal discs.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Nrg in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Nrg CG1634
One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders.
The probability of mating is reduced in ibx mutant females and the frequency of rejection behaviour towards courting males is increased.
Mutations causing structural defects in the adult central complex also alter larval behavioural phenotypes.
Three polypeptides of 155, 167 and 180kD are detected on Western blots; deglycosylation experiments reduce these to a single 155kD band; removal of N-linked high-mannose oligosaccharides causes a shift to about 162kD.
Encodes a protein that is likely to play a role in neural and glial cell adhesion in the developing Drosophila embryo.
Recessive alleles are embryonic lethals and show little or no evidence of Neuroglian expression; the overall structure of the CNS and PNS and in particular the peripheral nerve roots and CNS axon pathways develop in a relatively normal way in lethal embryos.
The gene is named "icebox" after the primary phenotype of mutants (the sexual receptivity of mutant females is lowered).