FlyBase Gene Report: Dmel\koi

General Information

Symbol

Dmel\koi

Species

D. melanogaster

Name

klaroid

Annotation Symbol

CG44154

Feature Type

protein_coding_gene

FlyBase ID

FBgn0265003

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Snapshot

klaroid (koi) encodes a protein involved in double-strand break repair and nuclear migration. [Date last reviewed: 2019-09-19]

Other Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

BEST:LD11166

Key Links

Genomic Location

Cytogenetic map

42D4-42D4

Sequence location

2R:6,811,019..6,818,199 [+]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:6811019..6818199

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables protein-membrane adaptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000308195

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in double-strand break repair via homologous recombination

inferred from mutant phenotype

(Ryu et al., 2015)

involved_in nuclear migration

inferred from mutant phenotype

(Kracklauer et al., 2007)

involved_in nucleus localization

inferred from mutant phenotype

(Tan et al., 2018, Elhanany-Tamir et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in nuclear envelope organization

inferred from biological aspect of ancestor with PANTHER:PTN000308195

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in nuclear envelope

inferred from direct assay

(Tan et al., 2018)

located_in nuclear periphery

inferred from direct assay

(Dialynas et al., 2010)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Kracklauer et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of meiotic nuclear membrane microtubule tethering complex

inferred from biological aspect of ancestor with PANTHER:PTN000308197

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nuclear envelope

inferred from biological aspect of ancestor with PANTHER:PTN000308195

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\koi for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

koi-RA

FBtr0335512

NM_001273809

4276

965

koi-RB

FBtr0335513

NM_136396

4100

881

koi-RC

FBtr0335514

NM_001273810

4233

965

koi-RD

FBtr0335515

NM_165491

2030

449

koi-RE

FBtr0335516

NM_165492

1856

365

koi-RF

FBtr0335517

NM_001273811

1989

449

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

koi-PA

FBpp0307480

109.7

965

7.15

NP_001260738

AGB93271

koi-PB

FBpp0307481

100.1

881

7.01

NP_610240

AAF57400

koi-PC

FBpp0307482

109.7

965

7.15

NP_001260739

AGB93272

koi-PD

FBpp0307483

51.8

449

9.44

NP_724502

AAG22207

koi-PE

FBpp0307484

42.2

365

9.65

NP_724503

AAM70833

koi-PF

FBpp0307485

51.8

449

9.44

NP_001260740

AGB93273

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

965 aa isoforms: koi-PA, koi-PC

449 aa isoforms: koi-PD, koi-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\koi using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

anterior endoderm anlage

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

Malpighian tubule primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic foregut

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic Malpighian tubule

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nuclear envelope

inferred from direct assay

(Tan et al., 2018)

located_in nuclear periphery

inferred from direct assay

(Dialynas et al., 2010)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Kracklauer et al., 2007)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\koi in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\koi

View exonic expression by tissue for Dmel\koi

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0265003

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3287

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of koi

Transgenic constructs containing regulatory region of koi

Deletions and Duplications ( 2 )

Disrupted in

Df(2R)CG3287-koi

(Technau and Roth, 2008)

Df(2R)Exel6050

(Kracklauer et al., 2007, Ryder, 2004.4.26)

Partially disrupted in

Df(2R)CG3287-koi

(Technau and Roth, 2008)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal, with Scer\GAL4^C57

koi^{UAS.PA.Tag:V5}

partially lethal - majority die, with Scer\GAL4^pnr-MD237

koi^GD1835

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

koi^GD1835

viable, with Scer\GAL4^Mef2.PR

koi^GD1835

Sterility

Allele

fertile

Other Phenotypes

Allele

size defective | third instar larval stage, with Scer\GAL4^C57

koi^{UAS.PA.Tag:V5}

size defective | third instar larval stage, with Scer\GAL4^Cg.PA

koi^GD1835

some die during pupal stage, with Scer\GAL4^pnr-MD237

koi^GD1835

visible, with Scer\GAL4^pnr-MD237

koi^GD1835

visible (with Df(2R)Exel6050)

visible | recessive

Phenotype manifest in

Allele

A1-7 dorsal acute muscle 1 | embryonic stage 16

koi⁸⁴

A1-7 dorsal acute muscle 2 | embryonic stage 16

koi⁸⁴

A1-7 dorsal oblique muscle 1 | embryonic stage 16

koi⁸⁴

A1-7 dorsal oblique muscle 2 | embryonic stage 16

koi⁸⁴

abdominal 1 lateral transverse muscle, with Scer\GAL4^Mef2.PU

koi^HMS02172

embryonic/larval hypodermal muscle | third instar larval stage

koi^CB04483

embryonic/larval hypodermal muscle | third instar larval stage, with Scer\GAL4^C57

koi^{UAS.PA.Tag:V5}

embryonic/larval hypodermal muscle | third instar larval stage (with Df(2R)Exel6050)

koi⁸⁴

eye

eye (with Df(2R)Exel6050)

fat cell | third instar larval stage, with Scer\GAL4^Cg.PA

koi^GD1835

microtubule | third instar larval stage, with Scer\GAL4^C57

koi^{UAS.PA.Tag:V5}

microtubule | third instar larval stage, with Scer\GAL4^Cg.PA

koi^GD1835

microtubule organizing center | third instar larval stage, with Scer\GAL4^Cg.PA

koi^GD1835

myotube | third instar larval stage

koi⁸⁰

nucleus, with Scer\GAL4^Mef2.PU

koi^HMS02172

nucleus, with Scer\GAL4^twi.PU

koi^HMS02172

nucleus & cone cell & eye disc (with Df(2R)Exel6050)

koi⁸⁰

nucleus & eye photoreceptor cell & eye disc

koi⁸⁰

nucleus & eye photoreceptor cell & eye disc (with Df(2R)Exel6050)

koi⁸⁰

nucleus | third instar larval stage

nucleus | third instar larval stage, with Scer\GAL4^C57

koi^{UAS.PA.Tag:V5}

nucleus | third instar larval stage, with Scer\GAL4^Cg.PA

koi^GD1835

nucleus | third instar larval stage (with Df(2R)Exel6050)

koi⁸⁴

rhabdomere (with Df(2R)Exel6050)

koi⁸⁰

trichogen cell, with Scer\GAL4^pnr-MD237

koi^GD1835

ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^Mef2.PU

koi^HMS02172

ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^twi.PU

koi^HMS02172

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\SUN1

7 of 15

Yes

Hsap\SUN2

6 of 15

Yes

Hsap\SUN3

4 of 15

Yes

Hsap\SPAG4

3 of 15

Yes

Hsap\SUN5

3 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Sun1

7 of 15

Yes

Mmus\Sun2

6 of 15

Yes

Mmus\Sun3

5 of 15

Yes

Mmus\Sun5

3 of 15

Mmus\Spag4

2 of 15

Rattus norvegicus (Norway rat) (6)

Rnor\Sun1

6 of 13

Yes

Rnor\Sun2

5 of 13

Yes

Rnor\Sun3

4 of 13

Yes

Rnor\LOC100911109

3 of 13

Yes

Rnor\Spag4

3 of 13

Yes

Rnor\Sun5

3 of 13

Xenopus tropicalis (Western clawed frog) (3)

Xtro\LOC100127596

4 of 12

Yes

Xtro\sun1

4 of 12

Yes

Xtro\sun2

1 of 12

Yes

Danio rerio (Zebrafish) (3)

Drer\sun1

8 of 15

Yes

Drer\si:dkey-92f12.2

7 of 15

Yes

Drer\zgc:152977

5 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\unc-84

7 of 15

Yes

Cele\sun-1

2 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\SUN1

5 of 9

Yes

Atha\SUN2

5 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\MPS3

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\sad1

6 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091901ZB )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA17191

Drosophila persimilis

Dper\GL11123

Drosophila willistoni

Dwil\GK22144

Drosophila virilis

Dvir\GJ22183

Drosophila mojavensis

Dmoj\GI19305

Drosophila grimshawi

Dgri\GH21760

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915054D )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17007549

Glossina morsitans

Tsetse fly

Gmor\GMOY003658

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11497

Mayetiola destructor

Hessian fly

Mdes\Mdes002921

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007475

Anopheles darlingi

American malaria mosquito

Adar\ADAC006652

Anopheles gambiae

Malaria mosquito

Agam\AGAP007311

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ016365

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W079N )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA000904

Bombyx mori

Silkmoth

Bmor\BGIBMGA005625

Danaus plexippus

Monarch butterfly

Dple\DPOGS211953-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS200302-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002917

Heliconius melpomene

Postman butterfly

Hmel\HMEL017775

Apis florea

Little honeybee

Aflo\2948645908

Apis mellifera

Western honey bee

Amel\GB47112

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10085

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP24205

Bombus terrestris

Buff-tailed bumblebee

Bter\15206945

Bombus terrestris

Buff-tailed bumblebee

Bter\15213924

Linepithema humile

Argentine ant

Lhum\LH12461

Linepithema humile

Argentine ant

Lhum\LH24690

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001cde

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0030fb

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0030fa

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG037206

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008867

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_08226

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07515

Tribolium castaneum

Red flour beetle

Tcas\TC030748

Tribolium castaneum

Red flour beetle

Tcas\TC005696

Pediculus humanus

Human body louse

Phum\PHUM418710

Pediculus humanus

Human body louse

Phum\PHUM327380

Pediculus humanus

Human body louse

Phum\PHUM235450

Rhodnius prolixus

Kissing bug

Rpro\RPRC003586

Rhodnius prolixus

Kissing bug

Rpro\RPRC003659

Cimex lectularius

Bed bug

Clec\CLEC009956

Cimex lectularius

Bed bug

Clec\CLEC005742

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006847

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11140

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_05035

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_06419

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X017K )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR003640

Ixodes scapularis

Black-legged tick

Isca\ISCW018754

Stegodyphus mimosarum

African social velvet spider

Smim\KFM60333.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur18g02650

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_62314

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_113732

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0AZ8 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000025727

Gallus gallus

Domestic chicken

Ggal\LOC769743

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000026668

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000003781

Gallus gallus

Domestic chicken

Ggal\LOC776146

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000000443

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000027003

Gallus gallus

Domestic chicken

Ggal\LOC100858507

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000025763

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000027815

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000028921

Gallus gallus

Domestic chicken

Ggal\LOC770405

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000026637

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000027290

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000028230

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

3 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SUN5; Sad1 and UNC84 domain containing 5

model of spermatogenic failure 16

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SUN1; Sad1 and UNC84 domain containing 1

7 of 15

607723

SUN2; Sad1 and UNC84 domain containing 2

6 of 15

613569

SUN3; Sad1 and UNC84 domain containing 3

4 of 15

618984

SPAG4; sperm associated antigen 4

3 of 15

603038

SUN5; Sad1 and UNC84 domain containing 5

3 of 15

613942

SPERMATOGENIC FAILURE 16; SPGF16

spermatogenic failure 16

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

koi - ari-1

enzymatic study, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Tan et al., 2018)

koi - park

anti tag coimmunoprecipitation, anti tag western blot

(Tan et al., 2018)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

koi

enhanceable

kud

(Ding et al., 2017)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

kud

suppressible

koi

(Ding et al., 2017)

kud

enhanceable

koi

(Ding et al., 2017)

shot

suppressible

koi

(Sun et al., 2019)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0265003

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42D4-42D4

Sequence location

2R:6,811,019..6,818,199 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42D4-42D4

Limits computationally determined from genome sequence between P{lacW}ubl^k03203&P{PZ}CG3287⁰⁹⁹⁶⁷ and P{EP}EP2303&P{EP}EP2105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Bloomington

26265

P{UAS-koi.gfp}1, w^*

26266

w^*; P{UAS-koi.gfp}3

25105

w^*; TI{TI}koi⁸⁰

42188

y¹ w^*; Mi{MIC}koi^MI06834

80132

y¹ w^*; PBac{UAS-koi-PA.V5}VK00037

51525

y¹ w^*; P{PTT-GB}koi^CB04483

20000

y¹ w^67c23; P{EPgy2}koi^EY03560

11776

P{PZ}koi⁰⁹⁹⁶⁷, ms(2)42D⁰⁹⁹⁶⁷ cn¹/CyO

40924

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02172}attP40

VDRC

v108236

P{KK106748}VIE-260B

v3990

w¹¹¹⁸; P{GD1835}v3990

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (309)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE12054

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0265003

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35594

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: koi CG18584

(Kracklauer et al., 2007, Kracklauer et al., 2007)

Source for database merge of

Source for merge of: BEST:LD11166 CG3287

(Levis, 2001.9.10)

Source for merge of: CG3287 koi

(FlyBase Genome Annotators, 2012)

Additional comments

Annotations CG3287 and CG18584 merged as CG44154 in release 5.47 of the genome annotation. Merge supported by RNA-seq junction data and functional analysis described in FBrf0210478.

(FlyBase Genome Annotators, 2012)

Other Comments

koi is essential for the recruitment of both Msp-300 and klar to the nuclear envelope.

(Technau and Roth, 2008)

The koi protein is found in the inner nuclear membrane in all cells in the eye disc, and is retained there through interactions with the nuclear lamina protein Lam.

(Kracklauer et al., 2007)

Origin and Etymology

Discoverer

Etymology

Named "klaroid" because of the striking similarities between the "klar" and "koi" mutant phenotypes.

(Kracklauer et al., 2007)

Identification

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35594

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0265003

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0265003

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3287

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35594

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC030748

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG44154

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0265003

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

BEST:LD11166

(Giot et al., 2003, Levis, 2001.9.10, Spradling et al., 1999)

CG18584

(Kracklauer et al., 2007, Kracklauer et al., 2007, Starr and Fischer, 2005, Starr and Han, 2003)

CG3287

(Kwon et al., 2013, Cammarato et al., 2011, Friedman et al., 2011, Hampoelz et al., 2011, Kallappagoudar et al., 2010, Keleman et al., 2009.8.5, Technau and Roth, 2008, Buszczak et al., 2007, Grieder et al., 2007)

CG44154

(FlyBase Genome Annotators, 2012, Ni et al., 2010.12.1)

Koi

(Azevedo and Baylies, 2020, Amaral et al., 2017, Caridi et al., 2017, Myat et al., 2015, Ryu et al., 2015)

n(2)09967

(Spradling, 1999.12.9)

Name Synonyms

CG18584

Klaroid

(Azevedo and Baylies, 2020, Rubin et al., 2020, Kim et al., 2015, Myat et al., 2015, Dialynas et al., 2012, Dialynas et al., 2010, Commisso and Boulianne, 2008, Elhanany and Volk, 2008)

klaroid

(Hughes et al., 2018, Petrovsky and Großhans, 2018, Huelsmann et al., 2013, Wesolowska et al., 2013, Elhanany-Tamir et al., 2012, Hampoelz et al., 2011, Technau and Roth, 2008, Kracklauer et al., 2007, Kracklauer et al., 2007)

Secondary FlyBase IDs

FBgn0033107
FBgn0028954
FBgn0033106

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (77)

The following external sites may use different assemblies or annotations than FlyBase.

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