FlyBase Gene Report: Dmel\Fatp2

long-chain fatty acid transmembrane transporter activity
RHEA 39283: very long-chain fatty acid-CoA ligase activity
RHEA 54536: long-chain fatty acid-CoA ligase activity

a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate (6.2.1.3)

RHEA 15421:

Summaries

Gene Group (FlyBase)

SLC27 FAMILY OF FATTY ACID TRANSPORTERS -: Solute carrier family 27 (SLC27) members are fatty acid transmembrane transporters. These proteins enhance the uptake of long-chain and very long chain fatty acids into the cells. (Adapted from FBrf0230430, PMID:26416577 and PMID:12856180).
FATTY ACYL-COA SYNTHETASES -: Fatty acyl-coenzyme A (CoA) synthetases catalyze the "activation" of fatty acids by their thioesterification to CoA. This is the initial reaction in fatty acid metabolism, allowing their participation in many fundamental anabolic and catabolic pathways. (Adapted from FBrf0237395.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Fatp2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KVJ6)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Genes with CDS overlap: isoforms of CG44252 and CG44253 share promoter, first exon and N-terminal coding sequences (28 residues); overlapping structure not conserved outside of insects.

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Fatp2-RB

FBtr0339110

NM_137905

2538

703

Fatp2-RD

FBtr0339109

NM_206204

2651

714

Fatp2-RE

FBtr0339108

NM_206203

2745

714

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Fatp2-PB

FBpp0308255

78.3

703

8.88

FBpp0308254

78.6

714

8.54

FBpp0308253

78.6

714

8.54

Q7KVJ6

NP_995925

AAF46943

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

714 aa isoforms: Fatp2-PD, Fatp2-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Fatp2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

4.67

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Fatp2 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Fatp2

View exonic expression by tissue for Dmel\Fatp2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37657/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0265187

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0265187

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30194

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Fatp2

Transgenic constructs containing regulatory region of Fatp2

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Fatp2^c01052

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

Fatp2^GD2170

viable, with Scer\GAL4^pnr-MD237

Fatp2^GD2170

viable, with Scer\GAL4^tin.CΔ4

Fatp2^GD16599

Sterility

Allele

fertile

Fatp2^c01052

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (23)

Hsap\SLC27A4

13 of 14

Yes

Hsap\SLC27A1

12 of 14

Yes

Hsap\SLC27A2

7 of 14

Yes

Hsap\SLC27A3

6 of 14

Yes

Hsap\SLC27A6

6 of 14

Hsap\SLC27A5

5 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (17)

Rnor\Slc27a4

13 of 14

Yes

Rnor\Slc27a1

12 of 14

Yes

Rnor\Slc27a2

7 of 14

Yes

Rnor\Slc27a3

6 of 14

Yes

Rnor\Slc27a6

6 of 14

Yes

Rnor\Slc27a5

5 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (18)

Mmus\Slc27a4

13 of 14

Yes

Mmus\Slc27a1

12 of 14

Yes

Mmus\Slc27a2

6 of 14

Mmus\Slc27a3

6 of 14

Yes

Mmus\Slc27a6

6 of 14

Mmus\Slc27a5

5 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\slc27a3

6 of 13

Yes

Xtro\slc27a4

6 of 13

Yes

Xtro\epgn

5 of 13

Yes

Xtro\slc27a2

4 of 13

Yes

Xtro\slc27a6

4 of 13

Yes

Xtro\supt20hl1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (19)

Drer\slc27a4

13 of 14

Yes

Drer\slc27a1a

12 of 14

Yes

Drer\slc27a1b

12 of 14

Yes

Drer\slc27a2a

7 of 14

Yes

Drer\slc27a2b

7 of 14

Yes

Drer\zgc:158482

7 of 14

Yes

Drer\zgc:101540

6 of 14

Yes

Drer\slc27a6

5 of 14

Yes

Drer\LOC568883

4 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (18)

Cele\acs-20

12 of 14

Yes

Cele\acs-22

12 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (17)

Agam\AgaP_AGAP001763

11 of 12

Yes

5 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (29)

Atha\AAE11

2 of 13

Yes

Atha\AT1G20490

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\FAT1

8 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPCC1827.03c

1 of 12

Yes

Escherichia coli (enterobacterium) (3)

Ecol\caiC

2 of 11

Yes

Ecol\fadD

1 of 11

Ecol\fadK

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Fatp2. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (23)

10 of 13

10 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

bgm

1 of 13

CG3961

1 of 13

CG6178

1 of 13

CG6432

1 of 13

hll

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

metabolic dysfunction-associated steatotic liver disease

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Fatp2^HMC03958

model of metabolic dysfunction-associated steatotic liver disease

CEA

(Pozo-Morales et al., 2024)

Fatp2^HMC02440

model of metabolic dysfunction-associated steatotic liver disease

CEA

(Pozo-Morales et al., 2024)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC27A4; solute carrier family 27 member 4

13 of 14

604194

ICHTHYOSIS PREMATURITY SYNDROME; IPS

SLC27A1; solute carrier family 27 member 1

12 of 14

600691

SLC27A2; solute carrier family 27 member 2

7 of 14

603247

SLC27A3; solute carrier family 27 member 3

6 of 14

604193

SLC27A6; solute carrier family 27 member 6

6 of 14

604196

SLC27A5; solute carrier family 27 member 5

5 of 14

603314

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Fatp2 - ksr

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

MIST (protein-protein) - An integrated Molecular Interaction Database

37657

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

(FlyBase Genome Annotators, 2012)

protein_coding_gene

(FlyBase Genome Annotators, 2012)

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-102

Cytogenetic map

59C2-59C2

Sequence location

2R:23,055,474..23,061,549 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

59C2-59C2

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-102

(FlyBase, 2016)

2-106.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

10356

w¹¹¹⁸; PBac{PB}Fatp2^c01052

77568

w¹¹¹⁸; PBac{IT.GAL4}Fatp2^0599-G4

56371

y¹ w^*; Mi{MIC}Fatp2^MI11764

76732

y¹ w^*; Mi{Trojan-GAL4.0}Fatp2^{MI11764-TG4.0}

77063

y¹ sc^* v¹ sev²¹; P{TKO.GS00851}attP40

78210

y¹ sc^* v¹ sev²¹; P{TOE.GS01388}attP40

44658

y¹ sc^* v¹ sev²¹; P{TRiP.HMC02440}attP2

55271

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03958}attP40

NIG-Fly

HMJ22037

y¹ v¹; P{TRiP.HMJ22037}attP40/CyO

HMC02440

y¹ sc^* v¹ sev²¹; P{TRiP.HMC02440}attP2

VDRC

v106140

P{KK102884}VIE-260B

v50235

w¹¹¹⁸; P{GD16599}v50235

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (7)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_RE60616

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0265187

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: CG13536 CG3037

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotation CG30194 split into CG44252 and CG44253 in release 5.49 of the genome annotation.

(FlyBase Genome Annotators, 2012)

The two annotations share a common 5' exon which encodes 28 N-terminal amino acid residues, but otherwise encode two distinct proteins.

(FlyBase Genome Annotators, 2012)

Comments: Annotations CG13536 and CG3037 merged as CG30194 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: Fatp2 CG44252

(FlyBase, 2017-)

Nomenclature comments

Featherstone, 2011 (FBrf0214274) identifies two SLC27/FATP genes: 'Fatp' (CG46149) and CG30194 (now called CG44252). Dourlen et al., 2015 (FBrf0230430) identify three D. melanogaster FATP genes: 'Fatp' (CG46149), CG30194 (i.e. CG44252) and CG3394. Orthology searches using human FATP genes confirm these three D. melanogaster hits. Therefore, re-named 'Fatp' as 'Fatp1', and CG44252 and CG3394 as 'Fatp2' and Fatp3', respectively.

(FlyBase, 2017-)

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG30194

(Baron et al., 2016, Dourlen et al., 2015, Sen et al., 2013, Featherstone, 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Liu and Lehmann, 2008, Watkins et al., 2007)

CG44252

(Anderson-Baron and Simmonds, 2018, Transgenic RNAi Project members, 2017-, Clandinin and Owens, 2016-, Gene Disruption Project members, 2016-, FlyBase Genome Annotators, 2012, Ni et al., 2010.12.1)

FATP2

(Pozo-Morales et al., 2024)

Fatp2

(Ceder and Fredriksson, 2022, Li et al., 2022, Juarez-Carreño et al., 2021)

SLC27A1/4

(Baron et al., 2016)

SLC27A2

(Anderson-Baron and Simmonds, 2018)

Name Synonyms

Fatty acid transport protein 2

Secondary FlyBase IDs

FBgn0050194

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 55 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37657

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KVJ6

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KVJ6

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37657/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0265187

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0265187

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG44252

MARRVEL_MODEL - MARRVEL (model organism gene)

37657

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0265187

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0265187

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30194

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0265187

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013521

MIST (protein-protein) - An integrated Molecular Interaction Database

37657

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

References (46)

The following external sites may use different assemblies or annotations than FlyBase.

4.67

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