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Gene Dmel\CG44254

General Information
Symbol	Dmel\CG44254	Species	D. melanogaster
Name		Annotation symbol	CG44254
Feature type	protein_coding_gene	FlyBase ID	FBgn0265189
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	96B6-96B6	Sequence location	3R:20,741,562..20,743,586 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG44254 (FBgn0265189). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: L-ascorbic acid binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors. Based on sequence similarity, it is predicted to be involved in the biological process: oxidation-reduction process. 3 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: Oxoglutarate/iron-dependent dioxygenase; Oxoglutarate/iron-dependent oxygenase, C-terminal degradation domain; Prolyl 4-hydroxylase, alpha subunit. Gene sequence location is 3R:20741562..20743586.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96B6-96B6
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG44254 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49 Genes with CDS overlap: isoforms of CG44254 and CG44255 share promoter, first exon and N-terminal coding sequences; overlapping structure not conserved outside of insects. CG44254 and CG44255 share 18 N-terminal amino acid residues.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG44254-RA FBtr0339113     1826   536
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG44254-PA   FBpp0308258   61.2   536   4.57
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Oxoglutarate/iron-dependent oxygenase, C-terminal degradation domain (IPR019601) Prolyl 4-hydroxylase, alpha subunit (IPR006620) Oxoglutarate/iron-dependent dioxygenase (IPR005123)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA696967   AI512448   AY058522   BF503304   BG632918   BG636012   BG640740   BG641381   BI367991   BI579792   BI580032   BI605533   BI617696   BI620848   CK132359   CK604388   CK604389   CO268011   CO282803   CO283649   CO306687   CZ478167   EC064021   EC077576   EC222521   EC231544   EV577546
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9I7H9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval midgut   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
[an error occurred while processing this directive] FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
[an error occurred while processing this directive]
modENCODE Development RNA-Seq
[an error occurred while processing this directive] modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
[an error occurred while processing this directive]
modENCODE Treatments RNA-Seq
[an error occurred while processing this directive]
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (Graveley et al., 2011) mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 CG44254GD742 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 CG44254GD742 visible, with Scer\GAL4pnr-MD237 CG44254GD742 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 CG44254GD742 trichogen cell, with Scer\GAL4pnr-MD237 CG44254GD742
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CG44254 Class Mutagen Stocks Known lesion CG44254e00272 piggyBac transposase 2 Yes
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG44254 Class Mutagen Stocks Known lesion CG44254GD742 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG44254KK115806 in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}CG44254e00272 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
	There is no information supporting a cellular component term for this gene. (Based on a review of available data on: 2012-10-26)
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
iron ion binding inferred from electronic annotation with InterPro:IPR006620, InterPro:IPR019601 (FlyBase Curators et al., 2004-) inferred from sequence model with InterPro:IPR006620, InterPro:IPR019601 (Curators, 2008--) L-ascorbic acid binding inferred from electronic annotation with InterPro:IPR006620, InterPro:IPR019601 (FlyBase Curators et al., 2004-) inferred from sequence model with InterPro:IPR006620, InterPro:IPR019601 (Curators, 2008--) oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors inferred from electronic annotation with InterPro:IPR005123, InterPro:IPR019601 (FlyBase Curators et al., 2004-) inferred from sequence model with InterPro:IPR005123, InterPro:IPR019601 (Curators, 2008--)
Biological Process
CV term Evidence References
oxidation-reduction process inferred from electronic annotation with InterPro:IPR005123, InterPro:IPR006620, InterPro:IPR019601 (FlyBase Curators et al., 2004-) inferred from sequence model with InterPro:IPR005123, InterPro:IPR006620, InterPro:IPR019601 (Curators, 2008--)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene (FlyBase Genome Annotators, 2012) protein_coding_gene (FlyBase Genome Annotators, 2012)
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
CG44254 - Arp2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG44254 - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG44254 - CG10641 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG44254 - CG5412 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) CG44254 - Cp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG44254 allele Gene References
External Data
Linkouts
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups - Dmel gene model changed subsequent to version 5.45
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6SQXGH Dipteran inclusive ortholog search EOG615GNW Insect inclusive ortholog search EOG6W6MR5 Arthropod inclusive ortholog search EOG6HHMHM Metazoa inclusive ortholog search EOG69PPRP
Orthologs in Drosophila Species (EOG6SQXGH)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG44255     Drosophila melanogaster fruit fly  Dmel\CG44254     Drosophila simulans   Dsim\GD18252   Y Drosophila sechellia   Dsec\GM23445   Y Drosophila erecta   Dere\GG12302   Y Drosophila yakuba   Dyak\GE10757   Y Drosophila ananassae   Dana\GF18678   Y Drosophila persimilis   Dper\GL23076   Y Drosophila willistoni   Dwil\GK14273   Y Drosophila virilis   Dvir\GJ10453   Y Drosophila mojavensis   Dmoj\GI23151   Y Drosophila grimshawi   Dgri\GH14258   Y
Orthologs in non-Drosophila Dipterans (EOG615GNW)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014305 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007498 Y Anopheles gambiae Malaria mosquito  Agam\AGAP002933 Y
Orthologs in non-Dipteran Insects (EOG6W6MR5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC413664 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007270 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12849 Y Atta cephalotes Leafcutter ant  Acep\ACEP22008 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO11844 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17685 Y Linepithema humile Argentine ant  Lhum\LH19326 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB20730 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB20737 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB20729 Y Solenopsis invicta Red fire ant  Sinv\SINV13026 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI006196 Y Bombyx mori Silkmoth  Bmor\BGIBMGA013061 Y Pediculus humanus Human body louse  Phum\PHUM466640 Y Tribolium castaneum Red flour beetle  Tcas\TC014607 Y
Orthologs in non-Insect Arthropods (EOG6HHMHM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_61607 Y Ixodes scapularis Deer tick  Isca\ISCW011109 Y
Orthologs in non-Arthropod Metazoa (EOG69PPRP)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\hpo-5 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025298gn Y Danio rerio Zebrafish  Drer\zgc:152927 Y Xenopus tropicalis Western clawed frog  Xtro\pigs Y Gallus gallus Domestic chicken  Ggal\PIGS Y Mus musculus House mouse  Mmus\Pigs Y Rattus norvegicus Norway rat  Rnor\Pigs Y Homo sapiens Human  Hsap\ENSG00000087111 Y
Human Orthologs (1)
Gene OMIM HGNC Hsap\ENSG00000087111
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	17833 w1118; PBac{RB}CG44254e00272/TM6B, Tb1
Harvard	- PBac{RB}CG44254e00272
VDRC	v3402 w1118; P{GD742}v3402
Genomic Clones ( 2 )
	BACR03I15 BACR33F18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 26 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD22945
Other clones	DPiM_LD22945
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT19263 GH16064 GM08815 LD44328 LP13823 RE53884 RE73874 RE74153 RH48108 RH52092 RH71172 SD11882 SD12709 SD13573
Other clones	45420 77107 149661 CK01.118B.A12 Dmel_dig_007176_0650_3120 EK089936 EK161243 EK164007 EK251908 UT01147
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	Annotation CG31120 split into CG44254 and CG44255 in release 5.49 of the genome annotation. The two annotations share a common 5' exon which encodes 18 N-terminal amino acid residues, but otherwise encode two distinct proteins. (FlyBase Genome Annotators, 2012) Annotations CG13644, CG6031, CG18761 merged as CG31120 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	DDBJ / EMBL / GenBank • AAL13751 CZ478167 UniProtKB/TrEMBL • Q9I7H9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Oxoglutarate/iron-dependent oxygenase, C-terminal degradation domain (IPR019601) Prolyl 4-hydroxylase, alpha subunit (IPR006620) Oxoglutarate/iron-dependent dioxygenase (IPR005123)
Linkouts
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG18761   CG31120 (Keleman et al., 2009.8.5) CG44254
Name Synonym
Secondary FlyBase IDs
	FBgn0042107 FBgn0051120
References ( 15 )
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] Guruharsha et al., 2011, Cell 147(3): Table S3. [FBrf0218395] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
FlyBase analysis	FlyBase Genome Annotators, 2012, Changes affecting gene model number or type in release 5.49 of the annotated D.melanogaster genome. Changes affecting gene model number or type in release 5.49 of the annotated D.melanogaster genome. [FBrf0219499] FlyBase Curators, 2010-, Gene Ontology (GO) annotation review. Gene Ontology (GO) annotation review. [FBrf0216433] Curators, 2008--, Assigning Gene Ontology (GO) terms by sequence similarity in FlyBase. Assigning Gene Ontology (GO) terms by sequence similarity in FlyBase. [FBrf0202953] FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886] FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]
Website	Fisher et al., 2012, BDGP insitu homepage BDGP insitu homepage [FBrf0219073]