FB2020_06, released Dec 22, 2020

Gene: Dmel\Inx3

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General Information
Symbol
Dmel\Inx3
Species
D. melanogaster
Name
Innexin 3
Annotation Symbol
CG1448
Feature Type
protein_coding_gene
FlyBase ID
FBgn0265274
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Snapshot
Innexin 3 (Inx3) encodes a gap junction protein that can form heteromeric gap junction channels via interaction with the product of Inx2. Loss of Inx3 leads to the destabilization of the products of ogre, Inx2 and shg at the plasma membrane, suggesting that these four proteins form a complex. [Date last reviewed: 2019-03-07]
Other Summaries
Also Known As

inx-3, Dm-inx3

Key Links
Genomic Location
Cytogenetic map
98E6-98F1
Sequence location
3R:28,853,606..28,859,497 [-]
Recombination map

3-97

RefSeq locus
NT_033777 REGION:28853606..28859497
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables gap junction channel activity
inferred from direct assay
(Stebbings et al., 2000)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables gap junction channel activity
inferred from biological aspect of ancestor with PANTHER:PTN000223951
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred by curator from GO:0005921
(Bohrmann and Zimmermann, 2008)
Biological Process (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in dorsal closure
inferred from mutant phenotype
(Giuliani et al., 2013)
involved_in intercellular transport
inferred from direct assay
(Stebbings et al., 2000)
involved_in phototransduction, visible light
inferred from genetic interaction with FLYBASE:ogre; FB:FBgn0004646
(Han et al., 2017)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in phototransduction
inferred from biological aspect of ancestor with PANTHER:PTN000934659
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in gap junction
inferred from direct assay
(Giuliani et al., 2013, Bohrmann and Zimmermann, 2008)
inferred from direct assay
(assigned by UniProt )
(Stebbings et al., 2000)
located_in lateral plasma membrane
inferred from direct assay
(Bohrmann and Zimmermann, 2008)
located_in plasma membrane
inferred from direct assay
(Lehmann et al., 2006)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
located_in gap junction
inferred from biological aspect of ancestor with PANTHER:PTN000223951
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in integral component of membrane
non-traceable author statement
(assigned by UniProt )
(Stebbings et al., 2000)
located_in plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000223951
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the pannexin family. (Q9VAS7)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
INNEXINS -
Gap junctions are intercellular channels that link the cytoplasm of neighboring cells facilitating cell-cell communication. In non-chordates they are composed of innexins. Innexins are four transmembrane proteins that assemble into hexamers around a central channel. The association of hemichannels on adjacent membranes forms the gap junction which allows the direct passage of ions and small molecules. Gap junctions may be formed from homo- or heteromultimers. (Adapted from FBrf0219962).
Protein Function (UniProtKB)
Structural components of the gap junctions. Essential for proper epithelial development of the epidermis.
(UniProt, Q9VAS7)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Inx3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.48

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Inx3-RA
FBtr0085350
NM_079991
1605
395
Inx3-RB
FBtr0330306
NM_001276121
2136
395
Inx3-RC
FBtr0345207
NM_001300646
1652
395
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Inx3-PA
FBpp0084719
45.4
395
8.58
NP_524730
AAF56822
Inx3-PB
FBpp0303338
45.4
395
8.58
NP_001263050
AGB96430
Inx3-PC
FBpp0311402
45.4
395
8.58
NP_001287575
AHN57574
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

395 aa isoforms: Inx3-PA, Inx3-PB, Inx3-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Heterooligomer of Inx2 (via cytoplasmic C-terminal region) and Inx3 (via cytoplasmic C-terminal region).

(UniProt, Q9VAS7)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Inx3 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
yolk nucleus
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
clypeo-labral primordium
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
hypopharynx proper primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic epipharynx
(Fisher et al., 2012)
embryonic foregut
(Fisher et al., 2012)
embryonic head epidermis
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic hypopharynx
(Fisher et al., 2012)
embryonic Malpighian tubule
(Fisher et al., 2012)
embryonic proventriculus
(Fisher et al., 2012)
embryonic ventral epidermis
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- 13
amnioserosa
(Giuliani et al., 2013)
ectoderm
(Giuliani et al., 2013)
oogenesis & adult stage | female
nurse cell
(Lautemann and Bohrmann, 2016)
follicle cell | apical | lateral
(Lautemann and Bohrmann, 2016)
centripetal follicle cell
(Lautemann and Bohrmann, 2016)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in gap junction
inferred from direct assay
(Giuliani et al., 2013, Bohrmann and Zimmermann, 2008)
inferred from direct assay
(assigned by UniProt )
(Stebbings et al., 2000)
located_in lateral plasma membrane
inferred from direct assay
(Bohrmann and Zimmermann, 2008)
located_in plasma membrane
inferred from direct assay
(Lehmann et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Inx3 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Inx3
Transgenic constructs containing regulatory region of Inx3
Deletions and Duplications ( 1 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (0)
No records found.
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (0)
No records found.
Rattus norvegicus (Norway rat) (0)
No records found.
Xenopus tropicalis (Western clawed frog) (0)
No records found.
Danio rerio (Zebrafish) (2)
Drer\panx1b
1 of 15
Yes
Yes
Drer\panx3
1 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (25)
Cele\inx-1
8 of 15
Yes
No
Cele\inx-2
8 of 15
Yes
Yes
Cele\inx-6
7 of 15
No
No
Cele\inx-17
7 of 15
No
Yes
Cele\unc-9
7 of 15
No
No
Cele\che-7
6 of 15
No
Yes
Cele\inx-3
6 of 15
No
Yes
Cele\inx-5
6 of 15
No
No
Cele\inx-7
6 of 15
No
Yes
Cele\inx-10
6 of 15
No
Yes
Cele\inx-11
6 of 15
No
Yes
Cele\inx-12
6 of 15
No
Yes
Cele\inx-13
6 of 15
No
No
Cele\inx-15
6 of 15
No
Yes
Cele\inx-16
6 of 15
No
Yes
Cele\inx-18
6 of 15
No
Yes
Cele\inx-19
6 of 15
No
Yes
Cele\inx-20
6 of 15
No
Yes
Cele\unc-7
6 of 15
No
No
Cele\eat-5
5 of 15
No
Yes
Cele\inx-8
5 of 15
No
No
Cele\inx-9
5 of 15
No
No
Cele\inx-14
4 of 15
No
No
Cele\inx-22
3 of 15
No
No
Cele\inx-21
2 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909E4 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00009853
Drosophila simulans
Dsim\GD18012
Drosophila sechellia
Dsec\GM12279
Drosophila erecta
Dere\GG12050
Drosophila yakuba
Dyak\GE10490
Drosophila ananassae
Dana\GF17986
Drosophila pseudoobscura pseudoobscura
Dpse\GA13015
Drosophila persimilis
Dper\GL23353
Drosophila willistoni
Dwil\GK13974
Drosophila virilis
Dvir\GJ23057
Drosophila mojavensis
Dmoj\GI24278
Drosophila grimshawi
Dgri\GH19243
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915056A )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17019502
Glossina morsitans
Tsetse fly
Gmor\GMOY001637
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11900
Aedes aegypti
Yellow fever mosquito
Aaeg\inx3
Anopheles darlingi
American malaria mosquito
Adar\ADAC010224
Anopheles gambiae
Malaria mosquito
Agam\inx
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W063Z )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA000452
Danaus plexippus
Monarch butterfly
Dple\DPOGS203272-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL007652
Apis florea
Little honeybee
Aflo\2211669172
Apis mellifera
Western honey bee
Amel\inx3
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17706
Bombus terrestris
Buff-tailed bumblebee
Bter\15213307
Linepithema humile
Argentine ant
Lhum\LH12198
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00062f
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG016016
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08519
Tribolium castaneum
Red flour beetle
Tcas\TC011709
Pediculus humanus
Human body louse
Phum\PHUM336890
Rhodnius prolixus
Kissing bug
Rpro\RPRC011950
Cimex lectularius
Bed bug
Clec\CLEC011695
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI004733
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_11442
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05ZJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_129101
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_56719
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G096J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000019846
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (7)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Inx2
5 of 10
Inx5
5 of 10
Inx6
5 of 10
ogre
5 of 10
shakB
5 of 10
zpg
5 of 10
Inx7
4 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Inx3
    surface plasmon resonance, predetermined participant
    (Lehmann et al., 2006)
    Inx3 - Inx2
    anti bait coimmunoprecipitation, western blot, surface plasmon resonance, predetermined participant, two hybrid, anti tag western blot
    (Lehmann et al., 2006)
    Inx3 - shg
    anti tag coimmunoprecipitation, western blot
    (Giuliani et al., 2013)
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Inx2
    enhanceable
    Inx3
    (Stebbings et al., 2000)
    External Data
    Subunit Structure (UniProtKB)
    Heterooligomer of Inx2 (via cytoplasmic C-terminal region) and Inx3 (via cytoplasmic C-terminal region).
    (UniProt, Q9VAS7 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map

    3-97

    Cytogenetic map
    98E6-98F1
    Sequence location
    3R:28,853,606..28,859,497 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    98E6-98F1
    Limits computationally determined from genome sequence between P{PZ}l(3)0648706487 and P{EP}EP3390EP3390
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    98E4-98E6
    (Davies, 1999.9.16, Stebbings, 1999.7.25)
    98E4-98E6
    (determined by in situ hybridisation)
    (Stebbings et al., 2000)
    Experimentally Determined Recombination Data
    Location

    3-97

    (FlyBase, 2016)

    3-94.3

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (10)
    Genomic Clones (17)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (101)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    Other clones
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     

    polyclonal

    (Bohrmann and Zimmermann, 2008, Lehmann et al., 2006)
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: Inx3 inx3

    (FlyBase, 2009-)
    Source for database merge of

    Source for merge of: inx3 CG1448

    (FlyBase, 1996-)

    Source for merge of: Inx3 anon- EST:fe2B11

    (FlyBase, 1992-)
    Additional comments

    Source for merge of inx3 CG1448 was sequence comparison ( date:000815 ).

    (FlyBase, 1996-)
    Other Comments

    dsRNA has been made from templates generated with primers directed against this gene.

    (Pilot et al., 2006)
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 30 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterPro - A database of protein families, domains and functional sites
    KEGG Genes - Molecular building blocks of life in the genomic space.
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (16)
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (77)