General Information
Symbol
Dmel\pAbp
Species
D. melanogaster
Name
poly(A) binding protein
Annotation Symbol
CG5119
Feature Type
FlyBase ID
FBgn0265297
Gene Model Status
Stock Availability
Gene Snapshot
In progress.Contributions welcome.
Also Known As
PABPC1, dPABP, l(2)SH1908, l(2)k10109, PABP55B
Genomic Location
Cytogenetic map
Sequence location
2R:18,140,078..18,147,191 [+]
Recombination map
2-84
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the polyadenylate-binding protein type-1 family. (P21187)
Molecular Function (see GO section for details)
Summaries
UniProt Contributed Function Data
Binds the poly(A) tail of mRNA.
(UniProt, P21187)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\pAbp or the JBrowse view of Dmel\pAbp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.44
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.50
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0086738
2433
634
FBtr0086740
3870
634
FBtr0086736
3127
634
Additional Transcript Data and Comments
Reported size (kB)
3.8 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0085917
69.9
634
9.80
FBpp0085919
69.9
634
9.80
FBpp0085915
69.9
634
9.80
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

634 aa isoforms: pAbp-PA, pAbp-PB, pAbp-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Interacts with gw; this interaction interferes with the binding of pAbp to eIF4G and is required for miRNA-mediated silencing. Interacts with larp.
(UniProt, P21187)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pAbp using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (25 terms)
Molecular Function (6 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:Paip2; FB:FBgn0038100
inferred from physical interaction with UniProtKB:Q8SY33
(assigned by UniProt )
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000610044
(assigned by GO_Central )
inferred from sequence or structural similarity
traceable author statement
inferred from sequence or structural similarity with SGD:S000000967
inferred from biological aspect of ancestor with PANTHER:PTN000610044
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000610044
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002768817
(assigned by GO_Central )
Biological Process (12 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
Cellular Component (7 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000610044
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000610046
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000610044
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000610044
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN002768817
(assigned by GO_Central )
Expression Data
Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
pAbp expression at embryonic stages 1-4 is localized to the posterior pole of the embryo.
pAbp transcript is expressed in cells immediately dorsal to and ventral to the dorsal/ventral compartment boundary of the wing disc; expression is limited to the anterior compartment. No expression is observed in the haltere disc.
pAbp transcripts are detected at all developmental stages on northern blots.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\pAbp in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 13 )
Transgenic Constructs ( 13 )
Deletions and Duplications ( 8 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
centriole & spermatocyte (with Df(2R)Pcl7B)
centrosome & spermatocyte (with Df(2R)Pcl7B)
spindle & spermatocyte
spindle & spermatocyte (with Df(2R)Pcl7B)
spindle & spermatocyte (with pAbpEP310)
spindle & spermatocyte (with pAbpk10109)
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
 
12 of 15
Yes
Yes
11 of 15
No
Yes
11 of 15
No
Yes
5 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
Yes
3 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
12 of 15
Yes
Yes
11 of 15
No
Yes
10 of 15
No
Yes
10 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
Yes
5 of 15
No
Yes
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (9)
11 of 13
Yes
Yes
10 of 13
No
Yes
10 of 13
No
Yes
9 of 13
No
Yes
5 of 13
No
Yes
5 of 13
No
Yes
5 of 13
No
Yes
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
8 of 12
Yes
Yes
8 of 12
Yes
Yes
6 of 12
No
Yes
Danio rerio (Zebrafish) (7)
12 of 15
Yes
Yes
11 of 15
No
Yes
11 of 15
No
Yes
9 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
12 of 15
Yes
Yes
11 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (8)
9 of 9
Yes
Yes
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
Yes
8 of 9
No
Yes
2 of 9
No
Yes
2 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (4)
12 of 15
Yes
Yes
5 of 15
No
Yes
3 of 15
No
Yes
1 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
11 of 12
Yes
Yes
1 of 12
No
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919067J )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503BR )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W02OG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02LP )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G03ZE )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 2 )
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please look at the Interaction Group reports for full details of the physical interactions
    protein-protein
    Interacting group
    Assay
    References
    RNA-protein
    Interacting group
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Interacts with gw; this interaction interferes with the binding of pAbp to eIF4G and is required for miRNA-mediated silencing. Interacts with larp.
    (UniProt, P21187 )
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map
    2-84
    Cytogenetic map
    Sequence location
    2R:18,140,078..18,147,191 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    55B5-55B9
    Limits inferred from genome sequence; Left limit from in situ hybridisation (FBrf0157097) Right limit from in situ hybridisation (FBrf0157097)
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    55B8-55B9
    55B-55B
    55B-55B
    (determined by in situ hybridisation)
    55B5-55B6
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (19)
    Genomic Clones (21)
    cDNA Clones (945)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: pAbp CG5119
    Source for database merge of
    Source for merge of: pAbp anon- EST:Posey247 anon- EST:Posey249
    Source for merge of: pAbp l(2)k10109
    Source for merge of: pAbp l(2)SH1908
    Source for merge of: pAbp BcDNA:LD24412
    Source for merge of: CG16803 anon- EST:fe2E5
    Source for merge of: pAbp CG16803
    Additional comments
    Annotation CG5119 and previously withdrawn annotation CG16803 merged in release 5.49 of the genome annotation; CG16803 corresponds to the 3' UTR of CG5119. Merge based on RNA-Seq expression data.
    Source for merge of pAbp BcDNA:LD24412 was a shared cDNA ( date:020730 ).
    Other Comments
    RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
    3 alleles of l(2)SH1908 recovered in a P-insertion screen.
    pAbp is required to connect the centrosome to the minus-ends of free microtubules.
    Annotation CG16803 eliminated in the July 2000 Celera release of annotated sequence.
    A sequence comparison and numerical analysis of the RRM-containing (RNA recognition motif) proteins suggests that functionally related RRM-containing proteins have significant sequence similarities in their RRMs.
    Isolated from a 3-12 hour embryo cDNA library using a human PABP cDNA probe.
    A pAbp cDNA has been cloned and sequenced.
    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 54 )
    Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    Linkouts
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
    DroID - A comprehensive database of gene and protein interactions.
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    KEGG Genes - Molecular building blocks of life in the genomic space.
    Synonyms and Secondary IDs (36)
    Reported As
    Symbol Synonym
    BcDNA:LD24412
    anon-EST:Posey247
    anon-EST:Posey249
    anon-EST:fe2E5
    l(2)SH2 1908
    Name Synonyms
    Poly(A) Binding Protein
    Poly(A) binding protein
    Poly(A)binding protein
    PolyA-binding protein
    Polyadenylate-binding protein
    anon-fast-evolving-2E5
    doppio fuso
    lethal (2) SH1908
    poly (A)-binding protein
    poly-A binding protein
    polyadenylate-binding protein
    Secondary FlyBase IDs
    • FBgn0261619
    • FBgn0003031
    • FBgn0021963
    • FBgn0027713
    • FBgn0027714
    • FBgn0063151
    • FBgn0065324
    • FBgn0025217
    • FBgn0040739
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (223)