• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\nac

General Information
Symbol	Dmel\nac	Species	D. melanogaster
Name	neuronally altered carbohydrate	Annotation symbol	CG9620
Feature type	protein_coding_gene	FlyBase ID	FBgn0265351
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	Gfr
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	84F10-84F10	Sequence location	3R:4,128,408..4,129,745 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene neuronally altered carbohydrate is referred to in FlyBase by the symbol Dmel\nac (CG9620, FBgn0265351). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: GDP-fucose transmembrane transporter activity. There is experimental evidence that it is involved in the biological process: Golgi vesicle transport; N-glycan fucosylation; protein O-linked fucosylation; grooming behavior; GDP-fucose transport; GDP-fucose import into Golgi lumen. 13 alleles are reported. The phenotypes of these alleles are annotated with: wing vein L3; wing vein L4. It has one annotated transcript and one annotated polypeptide. Protein features are: Domain of unknown function DUF250. Gene sequence location is 3R:4128408..4129745.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	nac: neuronally-altered-carbohydrate (F. Katz) Mutants show alteration or loss of a normally neuron-specific glycoconjugate; staining by anti-HRP antibodies in imaginal and adult neural tissue is eliminated. At 25 the mutant flies are viable and fertile; nac/nac females, however are sterile at 18. Under heat stress (37 for five minutes) they show abnormal jittery behavior. Developmental abnormalities, including defects in the assembly of the ommatidia and in the formation of the wing, appear at 18 in the homozygous offspring of heterozygous parents. Maternal effect embryos show loss of the anti-HRP glycan determinant and are lethal.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 84F10-84F10   Limits computationally determined from genome sequence between P{PZ}Mcm2rL074 and P{EP}EP833
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\nac for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nac-RA FBtr0081863  NM_141525   1225   337
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein nac-PA   FBpp0081352   38.1   337   9.24     NP_649782   AAF54215
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF250 (IPR004853)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF54215   BI243034   BI366724   BT003501 AAO39505   CO340169   EC247107   EC254595   EV585252   GH750109   GH753313   GH754381   GH754816   GH759894   GH771714   GH772902   GH780086   GH791127   GH793065   GH798579   GH804222   GH806627   GH815507   GH816947   GH817070   GH820127   GH821363   GH827490   GH834145   GH849022   GH854232   GH858359   GH858875   GH859091   GH861810   GH870749   GH877764   GH893119   GH906476   GH907581   GH910564   GH919544   GH920939   GH930978   GH931085
UniProtKB/Swiss-Prot	Q9VHT4
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Golgi apparatus inferred from direct assay (Geisler et al., 2012, Ishikawa et al., 2010, Ishikawa et al., 2005) Golgi-associated vesicle inferred from direct assay (Luehn et al., 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
[an error occurred while processing this directive] FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
[an error occurred while processing this directive]
modENCODE Development RNA-Seq
[an error occurred while processing this directive] modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
[an error occurred while processing this directive]
modENCODE Treatments RNA-Seq
[an error occurred while processing this directive]
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_03 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage, with Scer\GAL4elav.PU nacScer\UAS.cGa viable, with Scer\GAL4pnr-MD237 nacGD2857 Sterility Allele female sterile nac1 female sterile | recessive | cold sensitive nac1 Other Phenotypes Allele bang sensitive | heat sensitive nac1 behavior defective | heat sensitive nac1 behavior defective | recessive nac1 visible | dominant | maternal effect nac1 visible | dominant | paternal effect nac1 visible | recessive | cold sensitive nac1 Phenotype manifest in Allele ommatidium | maternal effect nac1 ommatidium | paternal effect nac1 sensory neuron & wing nac1 wing & sensory organ cell | ectopic nac2 nac3 nac4 wing | cold sensitive nac1 wing | heat sensitive nacGfr-1 nacGfr-2 wing | heat sensitive (with Df(3R)Exel6147) nacGfr-1 nacGfr-2 wing | heat sensitive (with nacGfr-1) nacGfr-2 wing | heat sensitive (with nacGfr-2) nacGfr-1 wing | maternal effect nac1 wing | paternal effect nac1 wing vein L3 nacGfr-2 wing vein L3 | heat sensitive nacGfr-1 wing vein L4 nacGfr-2 wing vein L4 | heat sensitive nacGfr-1
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of nac Class Mutagen Stocks Known lesion nac1 cold sensitive hypomorphic allele - genetic evidence ethyl methanesulfonate 1 Yes nac4 loss of function allele 1 -- nac2 loss of function allele 0 -- nac3 loss of function allele 0 -- nacEY01553 P-element activity 0 -- nacGfr-1 Delta2-3 0 Yes nacGfr-2 Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nac Class Mutagen Stocks Known lesion nacGD2857 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nacKK101122 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nacR125C.Scer\UAS in vitro construct - amino acid replacement in vitro construct - regulatory fusion 0 Yes nacScer\UAS.cGa in vitro construct - regulatory fusion 0 Yes nacScer\UAS.cIa in vitro construct - regulatory fusion 0 Yes nacScer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6147 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{UAS-nac.G} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}EY01553 NA P{EPgy2}nacEY01553 NA
Gene Ontology: Function, Process & Cellular Component ( 23 unique terms )
Terms Based on Experimental Evidence ( 23 terms )
Molecular Function
CV term Evidence References
NOT CMP-N-acetylneuraminate transmembrane transporter activity inferred from direct assay (Ishikawa et al., 2005) GDP-fucose transmembrane transporter activity inferred from direct assay (Luehn et al., 2004, Ishikawa et al., 2005) inferred from mutant phenotype (Geisler et al., 2012) NOT GDP-mannose transmembrane transporter activity inferred from direct assay (Ishikawa et al., 2005) NOT UDP-galactose transmembrane transporter activity inferred from direct assay (Ishikawa et al., 2005) NOT UDP-glucose transmembrane transporter activity inferred from direct assay (Ishikawa et al., 2005) NOT UDP-glucuronic acid transmembrane transporter activity inferred from direct assay (Ishikawa et al., 2005) NOT UDP-N-acetylgalactosamine transmembrane transporter activity inferred from direct assay (Ishikawa et al., 2005) NOT UDP-N-acetylglucosamine transmembrane transporter activity inferred from direct assay (Ishikawa et al., 2005)
Biological Process
CV term Evidence References
NOT CMP-N-acetylneuraminate transport inferred from direct assay (Ishikawa et al., 2005) GDP-fucose import into Golgi lumen inferred from direct assay (Geisler et al., 2012) inferred from mutant phenotype (Geisler et al., 2012) GDP-fucose transport inferred from direct assay (Luehn et al., 2004, Ishikawa et al., 2005) NOT GDP-mannose transport inferred from direct assay (Ishikawa et al., 2005) Golgi vesicle transport inferred from direct assay (Ishikawa et al., 2005) grooming behavior inferred from mutant phenotype (Phillis et al., 1993) N-glycan fucosylation inferred from mutant phenotype (Ishikawa et al., 2010, Ishikawa et al., 2005) protein O-linked fucosylation inferred from mutant phenotype (Ishikawa et al., 2005) NOT UDP-galactose transport inferred from direct assay (Ishikawa et al., 2005) NOT UDP-glucose transport inferred from direct assay (Ishikawa et al., 2005) NOT UDP-glucuronic acid transport inferred from direct assay (Ishikawa et al., 2005) NOT UDP-N-acetylgalactosamine transport inferred from direct assay (Ishikawa et al., 2005) NOT UDP-N-acetylglucosamine transport inferred from direct assay (Ishikawa et al., 2005)
Cellular Component
CV term Evidence References
Golgi apparatus inferred from direct assay (Geisler et al., 2012, Ishikawa et al., 2010, Ishikawa et al., 2005) Golgi-associated vesicle inferred from direct assay (Luehn et al., 2004)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
GDP-fucose import into Golgi lumen inferred by curator from GO:0005457|GO:0005794 (Ishikawa et al., 2005)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene   nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nac allele Gene References
External Data
Linkouts
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG61VKP1 Dipteran inclusive ortholog search EOG60ZR6B Insect inclusive ortholog search EOG6ZPCR2 Arthropod inclusive ortholog search EOG69P8F8 Metazoa inclusive ortholog search EOG6HTNM6
Orthologs in Drosophila Species (EOG61VKP1)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\nac     Drosophila simulans   Dsim\GD20878 Y   Drosophila sechellia   Dsec\GM26356 Y   Drosophila erecta   Dere\GG17462 Y   Drosophila yakuba   Dyak\GE24861 Y   Drosophila ananassae   Dana\GF16675 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21918 Y   Drosophila persimilis   Dper\GL24125 Y   Drosophila willistoni   Dwil\GK11707 Y   Drosophila virilis   Dvir\GJ10619 Y   Drosophila mojavensis   Dmoj\GI23412 Y   Drosophila grimshawi   Dgri\GH18913 Y
Orthologs in non-Drosophila Dipterans (EOG60ZR6B)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007481   Anopheles gambiae Malaria mosquito  Agam\AGAP002393   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ009824
Orthologs in non-Dipteran Insects (EOG6ZPCR2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Gfr   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG013708   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH18084   Atta cephalotes Leafcutter ant  Acep\ACEP19438   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18028   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16159   Linepithema humile Argentine ant  Lhum\LH20094   Pogonomyrmex barbatus Red harvester ant  Pbar\PB20988   Solenopsis invicta Red fire ant  Sinv\SINV25894   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002072   Bombyx mori Silkmoth  Bmor\BGIBMGA000854   Pediculus humanus Human body louse  Phum\PHUM283340   Tribolium castaneum Red flour beetle  Tcas\TC012326
Orthologs in non-Insect Arthropods (EOG69P8F8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_321827   Ixodes scapularis Deer tick  Isca\ISCW014762
Orthologs in non-Arthropod Metazoa (EOG6HTNM6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\C50F4.14   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006903gn   Danio rerio Zebrafish  Drer\slc35c1   Xenopus tropicalis Western clawed frog  Xtro\slc35c1   Gallus gallus Domestic chicken  Ggal\SLC35C1   Mus musculus House mouse  Mmus\Slc35c1   Rattus norvegicus Norway rat  Rnor\Slc35c1   Homo sapiens Human  Hsap\SLC35C1
Human Orthologs (1)
Gene OMIM HGNC Hsap\SLC35C1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20878 Drosophila sechellia Dsec\GM26356 Drosophila erecta Dere\GG17462 Drosophila yakuba Dyak\GE24861 Drosophila ananassae Dana\GF16675 Drosophila pseudoobscura pseudoobscura Dpse\GA21918 Drosophila persimilis Dper\GL24125 Drosophila willistoni Dwil\GK11707 Drosophila virilis Dvir\GJ10619 Drosophila mojavensis Dmoj\GI23412 Drosophila grimshawi Dgri\GH18913
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	6369 st1 nac1 6387 st1 nac4 es/TM6
VDRC	v105410 P{KK101122}VIE-260B v42623 w1118; P{GD2857}v42623
Genomic Clones ( 1 )
	BACR33N15 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 40 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE40567
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE52321
Other clones	Dmel_dig_006672_0709_1154 EP10136 RP003023416 RP003050173 RP003063155 RP003072452 RP003083325 RP003110410 RP003110481 RP003118765 RP003122065 RP003126708 RP003144582 RP003162972 RP003163723 RP003172743 RP003180200 RP003185545 RP003198222 RP003199218 RP003225004 RP003235463 RP003272656 RP003273073 RP003287418 RP003306607 RP003312749 RP003336257 RP003370293 RP003434703 RP003461094 RP003462249 RP003488654 RP003527422 RP003598477 RP003615732 RP003650372 RP003651633
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Gfr CG9620 (Ishikawa, 2005.3.7)
Source for database merge of	Source for merge of: nac Gfr (Geisler et al., 2012)
Additional comments
Other Comments
	Gfr may transport GDP-fucose from the cytosol into the golgi. (Ishikawa et al., 2005) N signaling deteriorates in Gfr mutants. (Ishikawa et al., 2005) The phenotypes of Fas1, Fas2, Fas3 and nac mutants were analysed in the developing wing: the axon tracts in the CNS for the most part remain unaltered, and none of the phenotypes are 100% penetrant, indicating a fine-tuning role for these molecules in both the PNS and CNS. (Whitlock, 1993) A mutation in nac affects anti-horse radish peroxidase (anti-HRP) epitope expression in imaginal nervous tissue causing behavioural defects. (Katz et al., 1988)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_141525 RefSeq (Proteins) • NP_649782 DDBJ / EMBL / GenBank • AAF54215 AAO39505 BI243034 BT003501 Entrez Gene - A searchable database of RefSeq genes. • 40981 UniProtKB/Swiss-Prot • Q9VHT4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF250 (IPR004853)
Linkouts
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG9620 (Roos et al., 2002, Luehn et al., 2004, Inlow and Restifo, 2004, Keleman et al., 2009.8.5) Gfr (Ayukawa et al., 2012, Geisler et al., 2012, Ishikawa et al., 2005, Vinci et al., 2008, Ishikawa et al., 2010) gfr (Ishikawa, 2005.3.7, Ishikawa et al., 2005) nac (Lindsley and Zimm, 1992, Geisler et al., 2012) unnamed (Snow et al., 1987)
Name Synonym	Gfr   Golgi GDP-fucose transporter (Ishikawa et al., 2005, Ishikawa et al., 2010) neuronally altered carbohydrate   neuronally-altered-carbohydrate (Lindsley and Zimm, 1992)
Secondary FlyBase IDs
	FBgn0002918 FBgn0037567
References ( 29 )
Generate a list of	All references relating to this gene ( 29 )
List References by type	Research paper  ( 14 ) Supplementary material  ( 3 ) Review  ( 2 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 2 ) FlyBase analysis  ( 1 ) Book  ( 1 )
Recent research papers ( 2 )
	Ayukawa et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(38): 15318--15323 Rescue of Notch signaling in cells incapable of GDP-L-fucose synthesis by gap junction transfer of GDP-L-fucose in Drosophila. [FBrf0219480] Geisler et al., 2012, J. Biol. Chem. 287(35): 29599--29609 The Drosophila Neurally Altered Carbohydrate Mutant Has a Defective Golgi GDP-fucose Transporter. [FBrf0219279] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...